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470 results on '"Nombela, César"'

18. Poacic acid, a β‐1,3‐glucan–binding antifungal agent, inhibits cell‐wall remodeling and activates transcriptional responses regulated by the cell‐wall integrity and high‐osmolarity glycerol pathways in yeast

Author

García, Raúl, primary, Itto‐Nakama, Kaori, additional, Rodríguez‐Peña, José Manuel, additional, Chen, Xiaolin, additional, Sanz, Ana Belén, additional, Lorenzo, Alba, additional, Pavón‐Vergés, Mónica, additional, Kubo, Karen, additional, Ohnuki, Shinsuke, additional, Nombela, César, additional, Popolo, Laura, additional, Ohya, Yoshikazu, additional, and Arroyo, Javier, additional

Published
2021
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20. L Aniversario

Author

Ribé, Daniel, Nombela, César, Andrade, Carmen, Aguirre, Fernando, Fisac, Miguel, Mas, Vicente, and García, Álvaro

Subjects
Engineering, Manufacturing, Engineering, Mechanical, Engineering, Civil, Engineering, Industrial, Engineering, Multidisciplinary, Engineering, Ocean, Computer Science, Software Engineering, Engineering, Aerospace, Engineering, Biomedical, Engineering, Marine
Abstract

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Published
2020

37. Signalling through the yeast MAPK Cell Wall Integrity pathway controls P-body assembly upon cell wall stress

Author

Ministerio de Economía y Competitividad (España), Comunidad de Madrid, European Commission, García, Raúl, Pulido, Veronica, Orellana-Muñoz, Sara, Nombela, César, Vázquez de Aldana, Carlos R., Rodríguez-Peña, José Manuel, Arroyo, Javier, Ministerio de Economía y Competitividad (España), Comunidad de Madrid, European Commission, García, Raúl, Pulido, Veronica, Orellana-Muñoz, Sara, Nombela, César, Vázquez de Aldana, Carlos R., Rodríguez-Peña, José Manuel, and Arroyo, Javier

Abstract

Post-transcriptional control of mRNA is a key event in the regulation of gene expression. From yeast to human cells, P-bodies are cytoplasmic RNA-protein aggregates that play an essential role in this process, particularly under stress conditions. In this work, we show that in the model yeast Saccharomyces cerevisiae cell wall stress induces the formation of these structures. This effect is dependent on multiple elements in the Cell Wall Integrity (CWI) MAPK signalling pathway, a signal transduction cascade responsible for the maintenance of cell integrity under adverse environmental conditions. Remarkably, P-body assembly requires the catalytic activity of the MAPK of the pathway, Slt2/Mpk1. In accordance with the control exerted by this signalling pathway, the timing of P-body formation is similar to that of the activation of the CWI pathway. Noticeably, mRNAs whose expression is regulated by this pathway localize in P-bodies after the cell is exposed to stress following a temporal pattern coincident with CWI pathway activation. Moreover, when these mRNAs are overexpressed in a mutant background unable to form visible P-bodies, the cells show hypersensitivity to agents that interfere with cell wall integrity, supporting that they play a role in the mRNA lifecycle under stress conditions.

Published
2019

42. Genome-wide survey of yeast mutations leading to activation of the yeast cell integrity MAPK pathway: Novel insights into diverse MAPK outcomes

Author

Arias Patricia, Díez-Muñiz Sonia, García Raúl, Nombela César, Rodríguez-Peña José M, and Arroyo Javier

Subjects
Biotechnology, TP248.13-248.65, Genetics, QH426-470
Abstract

Abstract Background The yeast cell wall integrity mitogen-activated protein kinase (CWI-MAPK) pathway is the main regulator of adaptation responses to cell wall stress in yeast. Here, we adopt a genomic approach to shed light on two aspects that are only partially understood, namely, the characterization of the gene functional catalog associated with CWI pathway activation and the extent to which MAPK activation correlates with transcriptional outcomes. Results A systematic yeast mutant deletion library was screened for constitutive transcriptional activation of the CWI-related reporter gene MLP1. Monitoring phospho-Slt2/Mpk1 levels in the identified mutants revealed sixty-four deletants with high levels of phosphorylation of this MAPK, including mainly genes related to cell wall construction and morphogenesis, signaling, and those with unknown function. Phenotypic analysis of the last group of mutants suggests their involvement in cell wall homeostasis. A good correlation between levels of Slt2 phosphorylation and the magnitude of the transcriptional response was found in most cases. However, the expression of CWI pathway-related genes was enhanced in some mutants in the absence of significant Slt2 phosphorylation, despite the fact that functional MAPK signaling through the pathway was required. CWI pathway activation was associated to increased deposition of chitin in the cell wall - a known survival compensatory mechanism - in about 30% of the mutants identified. Conclusion We provide new insights into yeast genes related to the CWI pathway and into how the state of activation of the Slt2 MAPK leads to different outcomes, discovering the versatility of this kind of signaling pathways. These findings potentially have broad implications for understanding the functioning of other eukaryotic MAPKs.

Published
2011
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48. The role of the cell wall in fungal pathogenesis

Author

Arana, David M., Prieto, Daniel, Román, Elvira, Nombela, César, Alonso‐Monge, Rebeca, and Pla, Jesús

Subjects
Mycoses, Cell Wall, Fungi, Animals, Carbohydrate Metabolism, Humans, Minireview
Abstract

Summary Fungal infections are a serious health problem. In recent years, basic research is focusing on the identification of fungal virulence factors as promising targets for the development of novel antifungals. The wall, as the most external cellular component, plays a crucial role in the interaction with host cells mediating processes such as adhesion or phagocytosis that are essential during infection. Specific components of the cell wall (called PAMPs) interact with specific receptors in the immune cell (called PRRs), triggering responses whose molecular mechanisms are being elucidated. We review here the main structural carbohydrate components of the fungal wall (glucan, mannan and chitin), how their biogenesis takes place in fungi and the specific receptors that they interact with. Different model fungal pathogens are chosen to illustrate the functional consequences of this interaction. Finally, the identification of the key components will have important consequences in the future and will allow better approaches to treat fungal infections.

Published
2009

49. Additional file 3: of Genomic profiling of fungal cell wall-interfering compounds: identification of a common gene signature

Author

García, Raúl, Botet, Javier, Rodríguez-Peña, José, Bermejo, Clara, Ribas, Juan, Revuelta, José, Nombela, César, and Arroyo, Javier

Abstract

Osmotic remediation by sorbitol of ZYM, CR and CAS hypersensitivity of mutant strains belonging to the “cell wall maintenance (CWM)” group. Minimal Inhibitory concentration (MIC) assays were carried out in 96-well microtiter plates as detailed in Methods either in the absence or in the presence of 0.8 M sorbitol. (PDF 1105 kb)

Published
2015
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50. Additional file 1: of Genomic profiling of fungal cell wall-interfering compounds: identification of a common gene signature

Author

García, Raúl, Botet, Javier, Rodríguez-Peña, José, Bermejo, Clara, Ribas, Juan, Revuelta, José, Nombela, César, and Arroyo, Javier

Abstract

Setting up of CAS, ZYM and CR hypersensitivity assays. Exponentially growing cells in YEPD medium of wild-type (BY4741) and the indicated mutant strains were inoculated in fresh YEPD medium containing different concentrations of CAS (a) or ZYM (b) in 96-well microtiter plates and incubated at 30 °C for 48 h. Cell growth was determined by measurement of absorbance at 595 nm (A595). (c) 1:10 serial dilutions of exponentially growing wild-type and mutant strains were spotted onto YEPD plates and YEPD plates supplemented with 50 μg/ml of CR and incubated at 30 °C for 3 days. (PDF 89 kb)

Published
2015
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