Search

Your search keyword '"Nollet, Kenneth E."' showing total 262 results
Start Over Author "Nollet, Kenneth E."
262 results on '"Nollet, Kenneth E."'

16. To the Editor

Author

Takahashi, Hideto, Ohira, Tetsuya, Yasumura, Seiji, Nollet, Kenneth E., Ohtsuru, Akira, Tanigawa, Koichi, Abe, Masafumi, and Ohto, Hitoshi

Published
2016

22. Can serum periostin predict bronchopulmonary dysplasia in premature infants?

Author

Go, Hayato, primary, Ono, Junya, additional, Ohto, Hitoshi, additional, Nollet, Kenneth E., additional, Sato, Kenichi, additional, Kume, Yohei, additional, Maeda, Hajime, additional, Chishiki, Mina, additional, Haneda, Kentaro, additional, Ichikawa, Hirotaka, additional, Kashiwabara, Nozomi, additional, Kanai, Yuji, additional, Ogasawara, Kei, additional, Sato, Maki, additional, Hashimoto, Koichi, additional, Nunomura, Satoshi, additional, Izuhara, Kenji, additional, and Hosoya, Mitsuaki, additional

Published
2021
Full Text
View/download PDF

24. Drinking Behavior and Mental Illness Among Evacuees in Fukushima Following the Great East Japan Earthquake: The Fukushima Health Management Survey

Author

Ueda, Yuka, Yabe, Hirooki, Maeda, Masaharu, Ohira, Tetsuya, Fujii, Senta, Niwa, Shin-ichi, Ohtsuru, Akira, Mashiko, Hirobumi, Harigane, Mayumi, Yasumura, Seiji, Abe, Masafumi, Yamashita, Shunichi, Kamiya, Kenji, Akashi, Makoto, Kodama, Kazunori, Ozasa, Kotaro, Nollet, Kenneth E., Niwa, Ohtsura, Matsui, Shiro, Kunii, Yasuto, Itagaki, Shuntaro, Shiga, Tetsuya, Iwasa, Hajime, Suzuki, Yuriko, Nakayama, Yoko, Ohta, Misao, Goto, Aya, Hisata, Mitsuru, Kawakami, Norito, Hosoya, Mitsuaki, Yagi, Akiko, Oiwaka, Yu-ichi, Horikoshi, Naoko, Kashiwazaki, Yu-ya, Takeda, Go, Hata, Tetsunobu, Suguimoto, Hiroyuki, Ito, Yukiko, Hino, Yuko, Hiyamizu, Keisuke, Kanke, Kuni, Yasuhara, Suzuko, Igarashi, Shizu, Kawamura, Ai, Matsuda, Aiko, Hara, Mariko, Kimura, Yuriko, Kumasaka, Yuki, Sasaki, Natusumi, Onji, Miho, Kurosawa, Ryuchi, and Mori, Fumiko

Published
2016
Full Text
View/download PDF

25. Sterilization efficacy of a new water‐free breast milk pasteurizer.

Author

Chishiki, Mina, Nishiyama, Kyoko, Suzutani, Tatsuo, Hiruta, Shun, Ichikawa, Hirotaka, Haneda, Kentaro, Maeda, Hajime, Shimizu, Hiromi, Kanai, Yuji, Ogasawara, Kei, Go, Hayato, Sato, Maki, Momoi, Nobuo, Nollet, Kenneth E., Ohto, Hitoshi, and Hosoya, Mitsuaki

Subjects
PREVENTION of infectious disease transmission, CYTOMEGALOVIRUSES, NEONATAL intensive care, BREAST milk, NEONATAL intensive care units, FOOD pasteurization, BACTERIAL growth, MICROBIOLOGICAL techniques, RESEARCH funding, STERILIZATION (Disinfection)
Abstract

Background: Breast milk, nature's optimum source of nutrition for infants, can contain undesirable microorganisms that cause severe morbidity. After an outbreak of multidrug‐resistant Escherichia coli among neonates receiving breast milk donated by another mother in our neonatal intensive care unit (NICU), we were motivated to develop a high‐grade breast milk pasteurizer (BMP) designed to thaw and pasteurize breast milk at 63°C for 30 min in a sealed bag without having to open the bag or immerse it in water. Methods: Pre‐existing bacteria and spiked cytomegalovirus (CMV) were measured pre‐ and post‐pasteurization in frozen breast milk donated by mothers of children admitted to the NICU. Results: Among 48 breast milk samples (mean ± standard deviation [SD]), pre‐existing bacterial counts of 5.1±1.1 × 104 colony forming units (cfu)/mL decreased to less than 10 cfu/mL (below detection level) in 45 samples after pasteurization for 30 min. In three samples, 10–110 cfu/mL persisted. As no CMV was detected in any of the 48 samples, CMV at ≥5 × 104 pfu/mL was spiked into 11 breast milk samples. After just 10 min of pasteurization, infectious CMV was not detected (threshold <50 pfu/mL) in any sample. Conclusion: A new BMP was shown to pasteurize milk effectively with more than a 3‐log reduction of microorganisms. Compared to conventional pasteurizers, this device reduces the effort involved in pasteurizing breast milk, avoids various contamination risks, and may reduce the risk of infectious disease transmission via breast milk. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

27. A practical and effective strategy in East Asia to prevent anti‐D alloimmunization in patients by C/c phenotyping of serologic RhD‐negative blood donors

Author

Ito, Shoichi, primary, Ohto, Hitoshi, additional, Ogiyama, Yoshiko, additional, Irino, Michiyo, additional, Omokawa, Susumu, additional, Shibasaki, Itaru, additional, Ogasawara, Kenichi, additional, Uchikawa, Makoto, additional, Nollet, Kenneth E., additional, and Flegel, Willy A., additional

Published
2021
Full Text
View/download PDF

28. Additional file 6 of Myeloproliferative neoplasm-driving Calr frameshift promotes the development of pulmonary hypertension in mice

Author

Minakawa, Keiji, Yokokawa, Tetsuro, Ueda, Koki, Nakajima, Osamu, Tomofumi Misaka, Kimishima, Yusuke, Wada, Kento, Tomita, Yusuke, Miura, Saori, Sato, Yuka, Mimura, Kosaku, Sugimoto, Koichi, Nakazato, Kazuhiko, Nollet, Kenneth E., Kazuei Ogawa, Ikezoe, Takayuki, Hashimoto, Yuko, Yasuchika Takeishi, and Ikeda, Kazuhiko

Abstract

Additional file 6. Fig. S6: Gene expressions. a-e RNA sequencing (RNAseq) in LSK (lineage–Sca1+c-Kit+) cells of an aliquot from 4 male mice of 3 months age in each sample from Calrins2/WT mice, Calrdel10/WT mice, and CalrWT/WT mice. a Principle component analysis. b Venn diagrams of upregulated and downregulated genes (> twofold) in LSK cells of Calrdel10/WT mice or Calrins2/WT mice relative to those of CalrWT/WT mice. c Pathway analysis by the Ingenuity Pathway Analysis software (Qiagen). All the pathways in the comparison analysis of canonical pathways with both Z score ≥|2| and p |10|-fold) in Calrdel10/WT mice relative to CalrWT/WT mice, including EDN1 that codes Endothelin-1 (allow), are shown. e Gene set enrichment analysis (GSEA) for the JAK-STAT pathway. NES indicates normalized enrichment score; FDRq, false discovery rate q value. f-g Introduction of FLAG-Tag-inserted human WT and del52 CALR constructs into a macrophage cell line, RAW 264.7. f Western blots. g The levels of Endothelin-1 mRNA (Edn1) were analyzed in RAW 264.7 cells introduced with CALR WT or del52 after incubation under normoxia (21% O2) or hypoxia (10% O2) for 24 h. Samples were taken from 3 wells for each experiment. Actb was used for normalization. The average value for cells introduced with WT CALR and incubated under normoxia was set to 1.

Published
2021
Full Text
View/download PDF

29. Additional file 5 of Myeloproliferative neoplasm-driving Calr frameshift promotes the development of pulmonary hypertension in mice

Author

Minakawa, Keiji, Yokokawa, Tetsuro, Ueda, Koki, Nakajima, Osamu, Tomofumi Misaka, Kimishima, Yusuke, Wada, Kento, Tomita, Yusuke, Miura, Saori, Sato, Yuka, Mimura, Kosaku, Sugimoto, Koichi, Nakazato, Kazuhiko, Nollet, Kenneth E., Kazuei Ogawa, Ikezoe, Takayuki, Hashimoto, Yuko, Yasuchika Takeishi, and Ikeda, Kazuhiko

Abstract

Additional file 5. Fig. S5: Relative Edn1 mRNA expression levels in the lung (n = 5, each). The average value for WT-R mice under normoxia was set to 1. (*P

Published
2021
Full Text
View/download PDF

30. Additional file 3 of Myeloproliferative neoplasm-driving Calr frameshift promotes the development of pulmonary hypertension in mice

Author

Minakawa, Keiji, Yokokawa, Tetsuro, Ueda, Koki, Nakajima, Osamu, Tomofumi Misaka, Kimishima, Yusuke, Wada, Kento, Tomita, Yusuke, Miura, Saori, Sato, Yuka, Mimura, Kosaku, Sugimoto, Koichi, Nakazato, Kazuhiko, Nollet, Kenneth E., Kazuei Ogawa, Ikezoe, Takayuki, Hashimoto, Yuko, Yasuchika Takeishi, and Ikeda, Kazuhiko

Abstract

Additional file 3. Fig. S3: Phosphorylation of STAT3 and expression of MPL, thrombopoietin receptor (TpoR). a Western blot of whole BM nuclear cells suspended in the absence of exogenous cytokines. b-c Flow cytometry gated with a lineage− fraction in BM cells. b Overall expression of cell-surface TpoR. Left: Histogram; right: mean fluorescence intensity (MFI). c Cell-surface expressions of TpoR and CALR. Left: heatmap plots; right: proportions of cell-surface TpoR+ cells in association with CALR expression (n = 3 in each experiment; *P

Published
2021
Full Text
View/download PDF

31. Additional file 1 of Myeloproliferative neoplasm-driving Calr frameshift promotes the development of pulmonary hypertension in mice

Author

Minakawa, Keiji, Yokokawa, Tetsuro, Ueda, Koki, Nakajima, Osamu, Tomofumi Misaka, Kimishima, Yusuke, Wada, Kento, Tomita, Yusuke, Miura, Saori, Sato, Yuka, Mimura, Kosaku, Sugimoto, Koichi, Nakazato, Kazuhiko, Nollet, Kenneth E., Kazuei Ogawa, Ikezoe, Takayuki, Hashimoto, Yuko, Yasuchika Takeishi, and Ikeda, Kazuhiko

Abstract

Additional file 1. Fig. S1: CALR proteins coded by ins2 and del10 frameshifts in murine Calr mimicked a feature of those coded by human type 2-like CALR mutations that generated novel C termini. a Western blot of BM cells using antibody specific for the CALR N terminus (CALR-N) or C terminus (CALR-C). b Isoelectric point (pI) in human and murine CALR proteins. c Alignment of C domains in mutant murine CALR from codon A352. Acidic and basic residues are in blue and red, respectively. #: the negatively charged amino acid stretches. †: the subjects of previously reported murine CALR mutants. d-f Identity and similarity between murine and human CALR frameshifts. The 2 MPN patients, with a mutated protein as CALR p.K375Rfs*52 (c.1124_1133del), matched the murine Calr del10 (p.K375Rfs*52 coded by Calr c.1124_1133del), although identity and similarity of the peptides were slightly different (f).

Published
2021
Full Text
View/download PDF

32. Additional file 8 of Myeloproliferative neoplasm-driving Calr frameshift promotes the development of pulmonary hypertension in mice

Author

Minakawa, Keiji, Yokokawa, Tetsuro, Ueda, Koki, Nakajima, Osamu, Tomofumi Misaka, Kimishima, Yusuke, Wada, Kento, Tomita, Yusuke, Miura, Saori, Sato, Yuka, Mimura, Kosaku, Sugimoto, Koichi, Nakazato, Kazuhiko, Nollet, Kenneth E., Kazuei Ogawa, Ikezoe, Takayuki, Hashimoto, Yuko, Yasuchika Takeishi, and Ikeda, Kazuhiko

Abstract

Additional file 8. Table S2: Oligonucleotides used in this study.

Published
2021
Full Text
View/download PDF

33. Additional file 2 of Myeloproliferative neoplasm-driving Calr frameshift promotes the development of pulmonary hypertension in mice

Author

Minakawa, Keiji, Yokokawa, Tetsuro, Ueda, Koki, Nakajima, Osamu, Tomofumi Misaka, Kimishima, Yusuke, Wada, Kento, Tomita, Yusuke, Miura, Saori, Sato, Yuka, Mimura, Kosaku, Sugimoto, Koichi, Nakazato, Kazuhiko, Nollet, Kenneth E., Kazuei Ogawa, Ikezoe, Takayuki, Hashimoto, Yuko, Yasuchika Takeishi, and Ikeda, Kazuhiko

Abstract

Additional file 2. Fig. S2: MPN-like phenotypes in knock-in mice with Calr frameshifts. a-b Body (a) and spleen (b) weights (n = 15—19). c BM nuclear cell counts (n = 4–6). d The proportions of BM CD71+Ter119+ erythroblasts, Gr1+ myeloid cells, B220+ B cells, and TCR+ T cells in flow cytometry (n = 3 in each). e–f Histology of BM (e) and spleens (f). g-h The numbers of megakaryocytes per high-power field (HPF) in BM (n = 3 in each) and spleens (n = 4—10). (*P

Published
2021
Full Text
View/download PDF

34. Additional file 7 of Myeloproliferative neoplasm-driving Calr frameshift promotes the development of pulmonary hypertension in mice

Author

Minakawa, Keiji, Yokokawa, Tetsuro, Ueda, Koki, Nakajima, Osamu, Tomofumi Misaka, Kimishima, Yusuke, Wada, Kento, Tomita, Yusuke, Miura, Saori, Sato, Yuka, Mimura, Kosaku, Sugimoto, Koichi, Nakazato, Kazuhiko, Nollet, Kenneth E., Kazuei Ogawa, Ikezoe, Takayuki, Hashimoto, Yuko, Yasuchika Takeishi, and Ikeda, Kazuhiko

Abstract

Additional file 7. Table S1: Frameshifts in CALR exon 9 on the COSMIC database in hematopoietic cancers.

Published
2021
Full Text
View/download PDF

35. Additional file 4 of Myeloproliferative neoplasm-driving Calr frameshift promotes the development of pulmonary hypertension in mice

Author

Minakawa, Keiji, Yokokawa, Tetsuro, Ueda, Koki, Nakajima, Osamu, Tomofumi Misaka, Kimishima, Yusuke, Wada, Kento, Tomita, Yusuke, Miura, Saori, Sato, Yuka, Mimura, Kosaku, Sugimoto, Koichi, Nakazato, Kazuhiko, Nollet, Kenneth E., Kazuei Ogawa, Ikezoe, Takayuki, Hashimoto, Yuko, Yasuchika Takeishi, and Ikeda, Kazuhiko

Abstract

Additional file 4. Fig. S4: Peripheral blood cell counts in the BMT recipients exposed to normoxia or chronic hypoxia for 3 weeks (n = 4–6). (*P

Published
2021
Full Text
View/download PDF

38. Biomarker Potential of the Soluble Receptor for Advanced Glycation End Products to Predict Bronchopulmonary Dysplasia in Premature Newborns

Author

Go, Hayato, primary, Ohto, Hitoshi, additional, Nollet, Kenneth E., additional, Sato, Kenichi, additional, Miyazaki, Kyohei, additional, Maeda, Hajime, additional, Ichikawa, Hirotaka, additional, Chishiki, Mina, additional, Kashiwabara, Nozomi, additional, Kume, Yohei, additional, Ogasawara, Kei, additional, Sato, Maki, additional, and Hosoya, Mitsuaki, additional

Published
2021
Full Text
View/download PDF

44. Serum Periostin Levels at Birth as a Predictor for Bronchopulmonary Dysplasia in Premature Infants.

Author

Go, Hayato, primary, Ono, Junya, additional, Ohto, Hitoshi, additional, Nollet, Kenneth E., additional, Sato, Kenichi, additional, Kume, Yohei, additional, Maeda, Hajime, additional, Chishiki, Mina, additional, Haneda, Kentaro, additional, Ichikawa, Hirotaka, additional, Kashiwabara, Nozomi, additional, Kanai, Yuji, additional, Ogasawara, Kei, additional, Sato, Maki, additional, Hashimoto, Koichi, additional, Nunomura, Satoshi, additional, Izuhara, Kenji, additional, and Hosoya, Mitsuaki, additional

Published
2020
Full Text
View/download PDF

48. Allo‐anti‐M: Detection peaks around 2 years of age, but may be attenuated by red blood cell transfusion.

Author

Tamai, Yoshiko, Ohto, Hitoshi, Yasuda, Hiroyasu, Takeshita, Akihiro, Fujii, Nobuharu, Ogo, Hiroaki, Yazawa, Yurika, Hato, Takaaki, Mitani, Kinuko, Suzuki, Keijiro, Yokohama, Akihiko, Kato, Yoko, Abe, Misao, Kumagawa, Midori, Ueda, Yasunori, Nollet, Kenneth E., Cooling, Laura, and Kitazawa, Junichi

Subjects
RED blood cell transfusion, ERYTHROCYTES, TEENAGERS, YOUNG adults
Abstract

Background: Anti‐M is frequently observed as a naturally occurring antibody of little clinical significance. Naturally occurring anti‐M is often found in children although the specific triggers of production, persistence, and evanescence of anti‐M have yet to be elucidated. Methods: In a retrospective, multicenter, nationwide cohort survey conducted from 2001 to 2015, alloantibody screening was performed before and after transfusion in 18,944 recipients younger than 20 years. Recipients were categorized into six cohorts based on their age at transfusion; within and among these cohorts, allo‐anti‐M was analyzed in regard to its production, persistence, and evanescence. Results: In 44 patients, anti‐M detected before and/or after transfusion was an age‐related phenomenon, with a median age of 2 years and an interquartile range of 1–3 years; anti‐M was most frequently detected in a cohort of children 1 to <5 years (0.77%, 31 of 4035). At least five patients were presumed to have concurrent infections. Among 1575 adolescents/young adults (15 to <20 years), no anti‐M was detected. Of 29 patients with anti‐M prior to transfusion, the antibody fell to undetectable levels in 17 recipients (89.5%, of whom at least 13 received only M‐negative red cells) after anywhere from 5 days to 5.8 years; anti‐M persisted in 2, and was not tested in 10. Only 15 recipients (0.08%) produced new anti‐M after transfusion. Conclusion: Naturally occurring anti‐M is a phenomenon of younger ages, predominantly between 1 and 3 years. After transfusion, it often falls to undetectable levels. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results