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Annual shedding of antlers by males is a characteristic feature of most deer species. Regrowth is very fast, reaching 2 cm/day for some species and making them an interesting model for studying tissue regeneration processes. The aim of this study was to compare the contents of calcium, magnesium, and phosphorus in antlers and cranial bones of European red deer from Western Pomerania, Poland. We obtained 30 antlers from three forestry districts that differed in the extent of environmental pollution with fluorine compounds, SO2, NOx, CO, and CO2 (Trzebież, Rokita and Gryfino). Deer were assigned to two age groups: from 2 to 4 years, and from 6 to 8 years. Powdered samples of cranial bones and antler base obtained with a dental drill were dissolved in nitric acid. Calcium and magnesium contents were measured by atomic absorption spectroscopy, while phosphorus was determined colorimetrically. The content of calcium was higher in antlers - (mean 133.96 mg/g) hardly surprising given the higher calcified cartilage and immature bone content in antler as opposed to cranial bone (mean 123.79 mg/g). Phosphorus content was slightly higher in bones than in antlers (84.62 mg/g and 83.58 mg/g, respectively), which suggests that Ca:P ratios are different in the mineral phase or that there is more P in bone compared to the antler matrix. No difference in magnesium content was noted (5.23 to 5.46 mg/g). Statistical analysis revealed significant differences depending on age of the animal and level of industrial pollution in the animal's habitat. [ABSTRACT FROM AUTHOR]

Background: Hemolysis during cardiopulmonary bypass may lead to acute kidney injury caused by an excessive amount of iron. The clinical usefulness of the measurement of total iron concentration in the urine with the use of the atomic absorption spectrometry method for early identification of patients with postoperative acute kidney injury is not well-established., Objectives: An observational, prospective study was conducted on a group of 88 pre-selected adult patients undergoing a planned coronary artery bypass grafting (CABG) procedure., Material and Methods: The amount and concentrations of total iron, creatinine and neutrophil gelatinaseassociated lipocalin (NGAL) were evaluated in urine samples. A comparative analysis of the evaluated biochemical parameters was performed in regard to the occurrence of acute kidney injury 48 h postoperatively., Results: Patients in the acute kidney injury group presented more advanced age (p = 0.01), preoperative myocardial infarction (p = 0.02), diuresis reduction (p = 0.04), and lower total iron levels in the 48-hour urine sample (p = 0.01). There was no difference when considering iron concentration in single urine samples in the study group., Conclusions: The sole result of total iron concentration in single urine samples is unreliable for the diagnosis of acute kidney injury after cardiac surgery. Decreased excretion of iron in the urine seems to be an important additional element in the multifactorial pathogenesis of acute postoperative kidney failure.

Numerous observations suggest a possible connection between the levels of Mg, Zn, Fe, and Zn and the incidence of depressive symptoms. Depression is two to three times more common in women than in men. The menopausal period is extremely conducive to depressive disorders. The aim of this study was to assess the severity of depressive symptoms in postmenopausal women depending on the levels of Mg, Zn, Ca, Cu, and Fe. The study included 198 healthy postmenopausal women at the average age of 56.26 ± 5.55 years. In the first part of the study, standardized research tools were used, namely the Primary Care Evaluation of Mental Disorders (PRIME-MD) and the Beck Depression Inventory (BDI). The second part involved biochemical analysis of Mg, Zn, Ca, Cu, and Fe levels in blood serum. The lowest Cu levels were observed in women without depressive symptoms (1.07 ± 0.22 mg/l) and the highest in those with severe depressive symptoms (1.19 ± 0.17 mg/l), (p ≤ 0.05). The lowest Mg levels were observed in women with depressive symptoms (14.28 ± 2.13 mg/l), and the highest in women without depressive symptoms (16.30 ± 3.51 mg/l), (p ≤ 0.05). The average serum Mg levels (15.75 ± 3.23 mg/l) decreased compared to the reference values (18.77-24 mg/l). What is striking is a potential relation between the levels of Mg and Cu and depressiveness. Our results indicate to a higher vulnerability to depression in a group of women with lower levels of Mg and higher levels of Cu., Competing Interests: The authors declare that they have no conflict of interest.

Approximately 30% of a woman's life is spent in the postmenopausal period. This is when steroid hormone deficiency is often accompanied by mineral homeostasis perturbations and deficiencies that could be related to the intensity of any clinical symptoms. The aim of this study was to assess how serum Mg and Zn levels in postmenopausal women correlate with climacteric symptoms, body mass index (BMI), and the time interval since the final menstruation. The study involved 171 healthy, postmenopausal women, who had had their final menstruation at least one year prior to the study and who did not use menopausal hormone therapy. Both hypomagnesaemia and hypozincaemia were detected in the postmenopausal women involved in this study. The analysis revealed statistically significant differences between serum Mg levels, depending on the time interval since the final menstruation (p<0.05). No statistically significant differences were found in serum Mg and Zn levels between women as regards the severity of the climacteric symptoms or BMI (p>0.05). In conclusion, serum Mg and Zn concentrations in postmenopausal women, not using MHT, were low. The average serum Mg levels decreased considerably with the time since the final menstruation. No correlation between BMI and worsening of climacteric symptoms and serum Mg and Zn concentrations in postmenopausal women, not using MHT was found.

The aim of this study was to assess the intensity of oxidative stress by measuring levels of lipid peroxidation products in the duodenum, jejunum and colon of rats infected with Hymenolepis diminuta and evaluate the effectiveness of protection against oxidative stress by measuring the glutathione levels and activity of anti-oxidant enzymes: superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase. In exposed rats we observed a significant increase of lipid peroxidation products in the duodenum and jejunum. A significant decrease in superoxide dismutase activity in all the examined parts of the digestive tract was observed. Additionally, rats from 16 to 40 days post H. diminuta infection (dpi) had a decreased catalase activity in the colon, while at 60dpi it increased. The glutathione peroxidase activity increased significantly in the colon at 60dpi. The increase in glutathione reductase activity was observed in the colon in rats 60dpi. There was a lack of changes in the levels of glutathione in the duodenum and a significant increase in its concentration in the jejunum and colon from 40 to 60dpi and from 16 to 40dpi, respectively. In this study we observed altered activity of anti-oxidant enzymes and glutathione level in experimental hymenolepidosis, as a consequence of oxidative stress. It may indicate a decrease in the efficiency of intestinal protection against oxidative stress induced by the presence of the parasite. The imbalance between oxidant and anti-oxidant processes may play a major role in pathology associated with hymenolepidosis., (Copyright © 2011 Elsevier Inc. All rights reserved.)

Aim: To compare the mineral content of the dentin of worn versus unworn teeth., Material and Methods: Coronal dentin samples were collected from twenty one premolar teeth extracted for prosthetic or periodontal indications, including 11 intact teeth and 10 teeth with a significant occlusal wear. Samples were placed in concentrated nitric acid and diluted 500 times for the analysis of calcium and magnesium content and 11 times for the analysis of zinc. Contents of calcium, magnesium and zinc ions were established by means of atomic absorption spectrometry in an oxygen acetylene flame. The content of phosphorus was established by means of spectrophotometry using a test basing on a reaction of colour phosphoric-molybdenic complex formation. Data normality was assessed with Shapiro-Wilk test. Student's t-test was used for all comparisons., Results: A statistically significantly higher content of magnesium and a lower Ca/Mg ratio were found in worn teeth at the 5% level. The concentrations of the other minerals analysed were not significantly different between the worn and intact teeth at the 5% level., Conclusion: Higher magnesium content in the dentin of human worn teeth may constitute a defence reaction to dentin exposure.

The mineral content of tooth hard tissue may influence the rate of decay change. Considering this fact, we aimed at examining if type 1 diabetes might be a contributing factor to the appearance of tooth decay. The experiment was conducted on female Wistar rats. To induce diabetes, rats were intravenously injected with 1 mL streptozocine 0.01 M citrate buffer. The control group of rats was injected with 1 mL 0.01 M citrate buffer only. After 10 days, teeth and blood serum samples were obtained. Fluoride concentration was determined by potentiometer method, and calcium and magnesium, by AAS. Serum concentrations of glucose and estradiol in the diabetic rats were significantly higher compared to the control group. In the experimental group, a statistically significant decrease of fluorine concentration in both teeth and serum were observed. Calcium and magnesium concentrations in blood serum and dental magnesium concentration were significantly higher in rats with type 1 diabetes compared with the control. A downward trend in the content of dental calcium in streptozotocin-induced diabetic rats was observed. The results obtained indicate that caries initiation and progression could be promoted by metabolic changes associated with diabetes affecting the mineral composition of tooth hard tissue.

Objective: This study aimed to determine the influence of high-dose soy isoflavones (daidzein and genistein) administered from prenatal life to sexual maturity on testosterone and estradiol levels, testicular and epididymal morphology, the number of epididymal spermatozoa, and mineral metabolism in rats., Methods: Pregnant Wistar rats received orally soy isoflavones, daidzein, and genistein at a dose of 200 mg/kg of body weight per day. After separating sucklings from their mothers, male rats received the same dose of isoflavones until reaching the age of sexual maturity, i.e., for 3 mo., Results: In the isoflavone-treated group, statistically significant decreased concentrations of zinc (determined using atomic absorption spectrophotometry) in blood serum and increased concentrations in bone were observed. The isoflavones induced changes in the morphology of the seminiferous epithelium of rat testes. However, there were no significant changes in the number of spermatozoa in the epididymis. The levels of estradiol in serum and cauda epididymis homogenates of rats receiving phytoestrogens were significantly higher than in the control group. No differences were observed in testosterone concentrations in the serum of treated and control rats. The testosterone levels in the homogenates of the treated rat testes were significantly lower than in the control group., Conclusion: The relatively mild effects of phytoestrogen administration on the morphology of testes and epididymides and the number of epididymal spermatozoa were observed despite the high dose used. The exposure of rats to genistein and daidzein during intrauterine life until sexual maturity influenced the mineral metabolism of the organism by significant decreases of Zn concentration in serum and increased Zn concentration in bones., (Copyright © 2011 Elsevier Inc. All rights reserved.)

Background/aims: Comparison of the iron status in patients who responded and did not respond to combination treatment with interferon alpha and ribavirin in chronic hepatitis C., Methodology: The study group comprised of 61 patients with chronic hepatitis C (genotype 1) treated with alpha 2b interferon and ribavirin. The iron metabolism was evaluated based on serum iron level, total iron binding capacity, transferrin saturation, serum ferritin concentration and hepatic iron concentration. In the evaluation of antiviral treatment efficacy biochemical and virological responses were taken into account., Results: End of treatment response was observed in 38 patients (62%). Significant differences in iron parameters were not observed between responders and non-responders. Also, sustained viral response, 6 months after treatment completion, was reached in 32 patients (52.5%). Iron metabolism parameters did not differ significantly in the group of sustained responders versus non- responders. Finally, ALT normalization was observed in 42 patients (68.9%). Again, no significant differences in iron status were observed between patients with and without biochemical response excluding significantly higher serum ferritin concentration in non-responders., Conclusions: Results of this study show that iron status does not significantly influence the efficacy of treatment with interferon and ribavirin in patients with chronic hepatitis C.

Drug addiction is a complex problem which leads to many somatic, psychic and social diseases. It is accompanied by the disturbed metabolism of various macro and micronutrients. The aim of this study was to assess serum magnesium concentration in drug-addicted patients and analyze whether Human Immunodeficiency Virus (HIV) infection and methadone treatment affect the level of serum magnesium in these patients. The examination was conducted in a group of 83 people - patients of Szczecin-Zdroje Psychiatric Hospital (Poland). They were 21 to 49 years old, and the mean age was 32 +/- 7 years. The control group consisted of 81 healthy individuals. Flame atomic-absorption spectrometry method was used to determine the magnesium concentration. The total serum magnesium concentration was calculated for the whole patient group, subgroups of women and men, a subgroup of people infected with HIV, and a subgroup receiving methadone substitution treatment. How magnesium behaves depending on age and addiction period, was checked. The mean concentration of magnesium in blood serum of the patients examined was 0.57 mmol/L, which was significantly lower than in the control group. In the subgroup of men it was 0.57 mmol/L, and in the subgroup of women - 0.55 mmol/L; the differences were not statistically significant. In the patient group nobody had the appropriate magnesium concentration in blood serum. No significant correlation was found between the magnesium concentration, age of the patients and addiction period. In the subgroup of seropositive people the mean concentration of magnesium was 0.55 mmol/L, and in the subgroup of non-infected patients - 0.58 mmol/L; the difference was not statistically significant. The mean concentration of magnesium in the subgroup treated with methadone was 0.59 mmol/L, and in the subgroup not involved in this type of therapy - 0.55 mmol/L; it was not a statistically significant difference.

Introduction: Introducing new methods of treatment in dentistry cause various alloys to appear in oral cavities. These alloys present in the oral cavity may generate an electrolytic cell, while saliva serves as a fine electrolytic conductor. Hence, the electropotentials that occur may initiate different illnesses., Material and Methods: The study was conducted among 25 patients. All of them have undergone treatment with the Viofor JPS system. The electropotentials in the oral cavity have been checked, as saliva's pH and selected levels of chemical elements have been estimated., Results: After having applied a series of treatments with the JPS system it was found that the electropotentials decreased, pH level of saliva increased, the content of copper in the non stimulated saliva fell, no alterations in the remaining elements before and after the treatments were observed.

Introduction: Enamel exhibits the highest degree of mineralization and is the hardest among tissues. Its non-organic material content is 96-98%, out of which 90% is in the form of hydroxyapatite crystals. Exchange of ions is an important property of hydroxyapatite with the hydroxyl ion (OH-) exchanging particularly easily for the fluoride ion (F-). F- ions are capable of stabilizing the structure of hydroxyapatite by reducing its solubility and in consequence increasing enamel resistance to caries. The aim of this work was to compare the content of fluoride in superficial layers of enamel obtained from permanent and deciduous teeth., Material and Methods: We collected 55 human permanent and 11 deciduous teeth without any evident damage to or discoloration of the enamel. Microsamples of enamel from the paragingival part on the buccal surface of the tooth were obtained using acid biopsy. Fluoride content was determined using an ion-selective electrode. Calcium content was measured with atomic absorption spectrometry., Conclusions: We found that enamel of deciduous teeth is more susceptible to acid digestion than in the case of permanent teeth, although the difference was not statistically significant. The content of fluoride decreased with increasing depth of digestion. Deciduous teeth contained significantly less fluoride in every layer studied in comparison with permanent teeth.

Purpose: The study was designed to evaluate the relationship between the content of fluoride, magnesium and phosphorus in enamel, depth of biopsy, and dentition status in postmenopausal women., Material and Methods: Dentition status in 81 postmenopausal women aged 48-70 years (mean 54.98 years) was determined using the Decayed, Missing and Filled Surfaces (DMFS) index. Double-layer superimposed acid biopsies were used for sampling labial enamel of upper permanent central incisor. Biochemical analysis was performed to determine the content of fluoride, magnesium, and phosphorus. Assuming that enamel contains 37% calcium, enamel mass, layer thickness, biopsy depth and concentrations of ions in the surface and subsurface layer were calculated. Correlation analysis was done to disclose relationships between the parameters studied., Conclusions: It was observed that the concentration of ions decreased with growing depth of biopsy. Decreasing depth of biopsy correlated with increasing concentration of fluorides and magnesium in surface and subsurface layer of enamel. The total depth of biopsy correlated more exactly with the concentration of fluorides and magnesium in subsurface layer than in surface layer. Increasing concentration of fluoride was associated with increasing concentration of magnesium. No correlation was observed between DMFS and the depth of enamel biopsy.

Purpose: The study was designed to evaluate the influence of tooth-brushing, milk consumption, hormone therapy, allergy, and cigarette smoking on the content of fluoride, calcium, magnesium and phosphorus in saliva., Material and Methods: Unstimulated saliva samples were collected from 100 women aged 48-70 years. Biochemical analysis was done to measure ion content. A questionnaire was used to determine hygiene and health habits. Correlation analysis was done to determine the relationships between parameters., Conclusions: A significant correlation was observed between ion content in saliva and frequency of tooth-brushing, smoking, and use of oral contraceptives. More frequent tooth-brushing was associated with reduced salivary content of calcium and phosphorus. Significantly higher salivary calcium levels were observed in smokers than non-smokers. Oral contraceptives produced significantly higher concentrations of fluoride ions in saliva. No correlation was noted between ion content in saliva and milk consumption, hormone replacement therapy (HRT), or allergy.

The aim of the study was to verify the hypothesis if copper could influence the activity of sodium-transporting systems in erythrocyte membrane that could be related to essential hypertension. The examined group of patients consisted of 15 men with hypertension. The control group was 11 healthy male volunteers. The Na+/H+ exchanger (NHE) activity in erythrocytes was determined according to Orlov et al. The activity of transporting systems (ATP-Na+/K+; co-Na+/K+/Cl-; ex-Na+/Li+; free Na+ and K+ outflow [Na+, K+-outflow]) was determined according to Garay's method. The concentration of copper in plasma was assessed using atomic absorption spectrometry. The activity of ATP-Na+/K+ (micromol/L red blood cells [RBCs]/h) in hypertensive patients was 2231.5 +/- 657.6 vs 1750.5 +/- 291 in the control (p < 0.05), the activity of co-Na+/K+/Cl- (micromol/L RBCs/h) in hypertensives was 171.3 +/- 77.9 vs 150.7 +/- 53.9 in the control (NS). Na+-outflow (micromol/L RBCs/h) in hypertensives was 118.3 +/- 51.6 vs 113.3 +/- 24.4 in the control (NS). The K+-outflow (micromol/L RBCs/h) in hypertensives was 1361.7 +/- 545.4 vs 1035.6 +/- 188.3 in the control (NS). The activity of ex-Na+/Li+ (micromol/L RBCs/h) in hypertensive patients was 266.1 +/- 76.1 vs 204.1 +/- 71.6 in the control (p < 0.05). NHE activity (mmol/L RBCs/h) in hypertensives was 9.7 +/- 2.96 vs 7.7 +/- 1.33 in the control (p < 0.05). In hypertensive patients, negative correlation was found between the activity of Na+/K+/Cl- co-transport and plasma copper concentration (Rs = -0.579, p < 0.05) and between the activity of ex-Na+/Li+ and plasma copper concentration (Rs = -0.508, p < 0.05). Plasma copper concentration significantly influences the activity of sodium transporting systems in erythrocyte membrane. Copper supplementation could be expected to provide therapeutic benefits for hypertensive patients.

This study was carried out on 48 patients with ESRD undergoing hemodialysis treatment (29 males and 19 females, mean age 50.8 years) and renal transplantation (15 females and 33 males, mean age 42.2 years). The results were compared with a group of 44 healthy persons (23 females and 21 males, mean age 49.5 years). The aim of our investigation was to examine the content of fluoride in the mixed unstimulated saliva of the patients undergoing renal replacement therapy and in the control group of healthy persons. We studied the influence of hemodialysis on saliva fluoride levels. Saliva samples were collected by the spitting method according to Navazesh. Samples were collected before and after hemodialysis session and once in graft recipients and controls. Fluoride concentrations were determined with an Orion fluoroselective electrode model 96-09. The fluoride level in healthy persons was 4.92 +/- 2.30 micromol/L. Before hemodialysis, the mean fluoride level was 9.63 +/- 3.90 micromol/L and decreased significantly to 7.52 +/- 2.71 micromol/L after hemodialysis (p < 0.0001). Saliva content of fluorides in patients before and after hemodialysis was significantly higher than in healthy subjects and kidney graft recipients (p < 0.0001). There was no significant difference in fluoride concentration between patients after kidney transplantation and controls. The results suggest the need for individual fluoride supplementation in chronically hemodialysed patients.

This work was designed to determine the content of fluorides, calcium, and magnesium in the superficial layer of enamel of adult teeth. The study group consisted of 25 patients, aged 18 to 35 years, appearing for dental treatment. Two microsamples of the superficial layer of enamel were collected from each patient with the acid biopsy technique. Samples were obtained from pulpectomized teeth treated endodontically and from teeth with necrotic pulp qualifying for endodontic treatment. Control samples were obtained from homonymous, caries-free teeth with vital pulp. The results of biochemical tests in the study group were compared with control teeth.

Fluorine and aluminum are able to pass through the blood-brain barrier and accumulate in the central nervous system (CNS) of exposed animals. Chronic intoxication is accompanied by behavioral disorders, degenerative changes, and abnormalities of aerobic metabolism of the neurons. Awareness of the role of aluminum in Alzheimer's disease stems from epidemiological studies demonstrating increased prevalence of this condition in areas with relatively high content of aluminum in drinking water. The uptake of aluminum in the gastrointestinal tract is decreased in the presence of iron, calcium, magnesium, phosphate, or fluoride. Many magnesium-containing enzymes are affected by aluminum, which is able to replace magnesium and thus reduce their activity. The purpose of this study was to determine the concentrations of fluorine, aluminum, and magnesium in some structures of the CNS of rats exposed to fluorine and aluminum in water. Our material consisted of 64 Wistar rats divided into eight equal groups. Groups I, II and III were female rats exposed, respectively, to 100 ppm fluorine ions, 300 ppm aluminum ions or both at same doses alternating every second day. Groups IA, IIA and IIIA consisted of male rats exposed like the respective female groups. Control groups K1--females and K2--males received distilled water ad libitum. Exposure lasted 31 days whereupon the animals were anesthetized with ketamine and sacrificed. The brain was collected and the cerebellum, brain cortex, and hippocampus were isolated. Concentrations of fluorine, aluminum, and magnesium were measured with prior mineralization of wet tissues in a microwave oven. Fluorine concentrations were determined with a potentiometric method and ion-selective electrode. Aluminum was measured with ICP (inductively coupled plasma) and magnesium with ASA (atomic absorption spectrometry). The highest concentrations of fluorine were observed in rats exposed to fluorine only. The same pattern was true for aluminum. Groups exposed alternatively to both elements demonstrated lower accumulation of fluorine whereas accumulation of aluminum did not change significantly. Apparently, aluminum reduced the availability of fluorine but there was no reciprocal effect. No significant changes in the concentrations of magnesium were noted, regardless of the brain structure or group. It can thus be concluded that exposure to fluorine, aluminum or both has little effect on the concentration of magnesium in the CNS of rats.

The objective of the study was to evaluate the concentration of fluoride in human enamel before and after bleaching using 10-15% carbamide peroxide and after application of the fluoride varnish. Microsamples of enamel were collected using acid biopsy and the concentration of fluoride in the superficial and underlying layer was assayed. No significant differences were observed in the concentration of fluoride in both layers of enamel before and after bleaching as well as after varnish application. Bleaching with 10-15% carbamide peroxide has no influence on the concentration of fluoride in enamel.

Tamoxifen, protactedly used, can cause disadvantageous changes in the bones and in the hard teeth tissues. The aim of this paper was to define to what extent tamoxifen given to the animals influences the structure of the mandible bone and the hard teeth tissues.

The actual paper presents the method and results of studies covering the mineral composition of the skulls of humans in the archaeological material stemming from different regions of Poland. The concentration of fluoride, zinc, iron, manganese, lead, calcium, magnesium and phosphorus was determined in 248 skulls. Distribution of individual burial sites providing the study material is presented in Fig. 1. The material was divided into four groups in relation to the place of deposition and in relation to the soil composition. Macro-, microelements determined by the method of atom absorption, phosphate by colourometric method, fluoride by potentiometric method. It has been disclosed that the bony material at the burial site is subjected to processes of fossilisation--replacing the organic matter by mineral one under definite soil conditions. The soil composition is the factor that influences the mineral composition of the bones in case of the following elements: zinc, manganese, lead, magnesium (Tab. 1). No influence was exerted by components contained in the soil on the determined concentrations of fluoride and iron in bones being explored from human skulls originating from archaeological excavation, undergo dynamic transformations in their mineral composition during their deposition in the soil. With the lapse of the time the content of fluoride (Tab. 2), magnesium (Tab. 7), calcium (Tab. 8), phosphate (Tab. 9) increases, while that of zinc (Tab. 3), iron (Tab. 4), manganese (Tab. 5) and lead (Tab. 6) decreases. The end concentration of elements in the archaeological bony material results from the following processes, namely: Cumulation, the example of which is the change in the concentration of fluoride, washing out the components of bone into the soil, to which the compounds of iron and manganese are subjected, compensations of concentrations of the bone soil border. That process took place in the case involving the changes in concentration of zinc, lead, magnesium, calcium and phosphate.

The blood serum of 420 children and young people between 5 and 18 years of age was examined to appoint the content of calcium, magnesium, zinc and copper in it. The examination was realized by AAS method. The calcium level was from 54.16 mg/l to 594.28 mg/l; an average was 148.66 +/- 64.61 mg/l. The magnesium level was from 7.78 mg/l to 66.26 mg/l; an average was 21.84 +/- 6.36 and was higher than data in literary output for children from other regions of Poland. The zinc level was from 0.19 mg/l to 2.94 mg/l, on an average 0.92 +/- 0.30 mg/l. The copper level was from 0.23 mg/l to 2.56 mg/l; average was 1.03 +/- 0.34 mg/l. The almost shortages in these bioelements were in children between 10 and 14, the least shortages were in children between 5 and 9. There were most of all children with high zinc level in blood serum in 10-14 years old group.