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Background: Prostate cancer is a malignant neoplasm of the male genitourinary system with the highest incidence worldwide. Susceptibility genes related to aggressiveness and prognosis, such as BRCA1/2, ATM, PTEN, have been identified. Currently, reports related to germline mutations in patients with prostate cancer in Latin American populations are very limited or absent. In the Mexican population, reports are also limited, especially in the context of metastatic prostate cancer. Determining the prevalence of these mutations is relevant to predict the potential aggressiveness of tumors and allow the use of targeted therapies, such as PARPi inhibitors. Objective: Determine the prevalence of germline mutations in patients with metastatic prostate cancer and establish their clinical characteristics at diagnosis. Material and Methods: Sixty-nine patients with metastatic PCa underwent testing and genetic analysis using the Comprehensive Multi-Cancer Hereditary Cancer Panel. The prevalence of germline mutations was assessed, and the cohort was divided into two groups for the evaluation and analysis of clinical characteristics between the mutated and non-mutated populations. Results: We identified mutations in 15 out of 69 patients (21.73%), while 54 patients (78.26%) had no mutations. Pathogenic mutations were observed in 15.9% of patients, Variants of Uncertain Significance (VUS) in 34.78%, and 5.79% had both. The most frequent mutations included ATM (11.54%), BRCA1 (11.54%), BRCA2 (7.69%), FANCA (7.69%), and FANCM (7.69%). No statistically significant differences were found in PSA levels, age at diagnosis, and resistance to castration between the two groups. Conclusions: Our study unveiled a mutation rate of 21.73%, marked by a significant prevalence of ATM, FANCA, FANCM, and Variants of Uncertain Significance (VUS). This pattern deviates from findings in other series, underscoring the necessity for improved access to clinical genetic testing in our population.

Refactoring is one of the techniques mostly employed by software developers to improve the quality attributes of their systems. However, little has been done to investigate how refactoring operations specifically aimed at improving the internal structure of software can impact its security. Refactoring usually entails different code change operations including the decomposition of classes, methods, and the reallocation of code elements. While this refinement aims to improve the internal design of a system, it might inadvertently disperse security-critical code elements throughout the codebase. Consequently, such dispersion could affect the software attack surface. To this end, this paper presents an empirical study conducted on 30 open-source software systems that were developed in Python, C, and Javascript. The study scrutinized two subsequent versions of each subject application to uncover the refactoring operations applied and the trend of the software attack surface. Specifically, the study focused on the injection or removal of bugs, code smells and other vulnerabilities aiming to discern the impact of refactorings on the software attack surface. Data was collected using well-known tools, namely SonarQube, RefDiff, and PyReff. The findings suggest that refactorings can have multiple impacts (i.e., positive, negative, or neutral) on bugs, code smells, and vulnerabilities. The findings further confirm that developers must be aware of the combination or sequence of refactoring operations that can improve software quality without compromising its security.

IntroductionAt the cellular level, acute temperature changes alter ionic conductances, ion channel kinetics, and the activity of entire neuronal circuits. This can result in severe consequences for neural function, animal behavior and survival. In poikilothermic animals, and particularly in aquatic species whose core temperature equals the surrounding water temperature, neurons experience rather rapid and wide-ranging temperature fluctuations. Recent work on pattern generating neural circuits in the crustacean stomatogastric nervous system have demonstrated that neuronal circuits can exhibit an intrinsic robustness to temperature fluctuations. However, considering the increased warming of the oceans and recurring heatwaves due to climate change, the question arises whether this intrinsic robustness can acclimate to changing environmental conditions, and whether it differs between species and ocean habitats.MethodsWe address these questions using the pyloric pattern generating circuits in the stomatogastric nervous system of two crab species, Hemigrapsus sanguineus and Carcinus maenas that have seen a worldwide expansion in recent decades.Results and discussionConsistent with their history as invasive species, we find that pyloric activity showed a broad temperature robustness (>30°C). Moreover, the temperature-robust range was dependent on habitat temperature in both species. Warm-acclimating animals shifted the critical temperature at which circuit activity breaks down to higher temperatures. This came at the cost of robustness against cold stimuli in H. sanguineus, but not in C. maenas. Comparing the temperature responses of C. maenas from a cold latitude (the North Sea) to those from a warm latitude (Spain) demonstrated that similar shifts in robustness occurred in natural environments. Our results thus demonstrate that neuronal temperature robustness correlates with, and responds to, environmental temperature conditions, potentially preparing animals for changing ecological conditions and shifting habitats.

Electronic Government (e-Government) systems constantly provide greater services to people, businesses, organisations, and societies by offering more information, opportunities, and platforms with the support of advances in information and communications technologies. This usually results in increased system complexity and sensitivity, necessitating stricter security and privacy-protection measures. The majority of the existing e-Government systems are centralised, making them vulnerable to privacy and security threats, in addition to suffering from a single point of failure. This study proposes a decentralised e-Government framework with integrated threat detection features to address the aforementioned challenges. In particular, the privacy and security of the proposed e-Government system are realised by the encryption, validation, and immutable mechanisms provided by Blockchain. The insider and external threats associated with blockchain transactions are minimised by the employment of an artificial immune system, which effectively protects the integrity of the Blockchain. The proposed e-Government system was validated and evaluated by using the framework of Ethereum Visualisations of Interactive, Blockchain, Extended Simulations (i.e. eVIBES simulator) with two publicly available datasets. The experimental results show the efficacy of the proposed framework in that it can mitigate insider and external threats in e-Government systems whilst simultaneously preserving the privacy of information.

In recent years, AI technologies have become indispensable in social and industrial development, yielding revolutionary results in improving labor efficiency, lowering labor costs, optimizing human resource structure, and creating new job demands. To reap the full benefits of responsible AI solutions in Africa, it is critical to investigate existing challenges and propose strategies, policies, and frameworks for overcoming and eliminating them. As a result, this study investigated the challenges of adopting responsible AI solutions in the Academia-Private sectors for Anglophone Africa through literature reviews, expert interviews, and then proposes solutions and framework for the sustainable and successful adoption of responsible AI.

Abstract Understanding biological responses to environmental fluctuations (e.g. heatwaves) is a critical goal in ecology. Biological responses (e.g. survival) are usually measured with respect to different time reference frames, i.e. at specific chronological times (e.g. at specific dates) or biological times (e.g. at reproduction). Measuring responses on the biological frame is central to understand how environmental fluctuation modifies fitness and population persistence. We use a framework, based on partial differential equations (PDEs) to explore how responses to the time scale and magnitude of fluctuations in environmental variables (= drivers) depend on the choice of reference frame. The PDEs and simulations enabled us to identify different components, responsible for the phenological and eco-physiological effects of each driver on the response. The PDEs also highlight the conditions when the choice of reference frame affects the sensitivity of the response to a driver and the type of join effect of two drivers (additive or interactive) on the response. Experiments highlighted the importance of studying how environmental fluctuations affect biological time keeping mechanisms, to develop mechanistic models. Our main result, that the effect of the environmental fluctuations on the response depends on the scale used to measure time, applies to both field and laboratory conditions. In addition, our approach, applied to experimental conditions, can helps us quantify how biological time mediates the response of organisms to environmental fluctuations.

Objectives: The aim of this study was to investigate the feasibility of saliva sampling as a non-invasive and safer tool to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and to compare its reproducibility and sensitivity with nasopharyngeal swab samples (NPS). The use of sample pools was also investigated. Methods: A total of 2107 paired samples were collected from asymptomatic healthcare and office workers in Mexico City. Sixty of these samples were also analyzed in two other independent laboratories for concordance analysis. Sample processing and analysis of virus genetic material were performed according to standard protocols described elsewhere. A pooling analysis was performed by analyzing the saliva pool and the individual pool components. Results: The concordance between NPS and saliva results was 95.2% (kappa 0.727, p = 0.0001) and 97.9% without considering inconclusive results (kappa 0.852, p = 0.0001). Saliva had a lower number of inconclusive results than NPS (0.9% vs 1.9%). Furthermore, saliva showed a significantly higher concentration of both total RNA and viral copies than NPS. Comparison of our results with those of the other two laboratories showed 100% and 97% concordance. Saliva samples are stable without the use of any preservative, and a positive SARS-CoV-2 sample can be detected 5, 10, and 15 days after collection when the sample is stored at 4 °C. Conclusions: The study results indicate that saliva is as effective as NPS for the identification of SARS-CoV-2-infected asymptomatic patients. Sample pooling facilitates the analysis of a larger number of samples, with the benefit of cost reduction.

Resumen: Objetivo: Desarrollar una metodología de evaluación de campos clínicos mediante la percepción de los médicos residentes. Material y métodos: Se identificaron 20 variables, que se agruparon en cuatro categorías: 1) profesor tutor; 2) organización hospitalaria; 3) complementos educativos, y 4) opinión global. Resultados: Se diseñó y validó una herramienta y escala de gradientes de idoneidad, encontrando el nivel de idoneidad aceptable en el 55% de los campos clínicos, en contraste con un 45% ubicado en el nivel no aceptable. Conclusión: Esta herramienta permitió medir y comparar por gradientes la calidad de los campos clínicos para entrenamiento de los médicos residentes. Abstract: Objective: To develop a method for the evaluation of clinical sites through the perspective of medical residents. Material and method: A set of 20 variables were selected and grouped into the following categories: (i) teaching staff; (ii) hospital organisation; (iii) educational program, and (iv) overall opinion. Results: A gradient scale of the suitability of the clinical site was designed, and validated, with 55% of the clinical sites being at an acceptable level and 45% being unacceptable below. Conclusion: This tool permitted the quality of the clinical sites used by the medical residents to be measured using a gradient scale. Palabras clave: Campos clínicos, Gestión educativa, Médicos residentes, Programas de residencia médica, Keywords: Clinical sites, Education administration, Medical residents, Medical residency programs

Objective The present study aimed to identify Mollicutes infection in the reproductive system. We also examined the microbiological, biochemical, and antimicrobial profiles of Mollicutes infection, which are potentially associated with clinical reproductive abnormalities causing infertility in couples. Methods Thirty-seven couples who were attending an infertility clinic were enrolled. Detection of genital mycoplasmas was performed in cervicovaginal samples or male urethral swabs. Microbiological culture and biochemical and antimicrobial profiles were characterized using a Mycoplasma kit. The results were associated with reproductive abnormalities, as assessed by medical specialists from the infertility clinic. Results Up to 28.3% of all biological samples (n = 74) showed positive cultures. Bacterial isolates were Ureaplasma urealyticum (71.4%), Mycoplasma hominis (9.5%), or coinfections (19%). Most Mollicutes showed significant resistance to fluoroquinolones, macrolides, and tetracycline; and showed susceptibility to doxycycline, josamycin, and pristinamycin. The presence of resistant strains to any antibiotic was significantly associated with genital abnormalities (χ 2 test, relative risk = 11.38 [95% confidence interval: 5.8–22.9]), particularly in women. The highest statistical association was found for macrolide-resistant strains. Conclusion The microbiological antibiotic resistance profile is epidemiologically associated with abnormalities of the reproductive system in couples attending an infertility clinic.

eBaViR is a virtual rehabilitation system, which has been developed for balance rehabilitation for patients suffering from acquired brain injury. It is a game-based system that uses a low-cost interface, the Nintendo Wii Balance Board. The games have been specifically designed with the help of experts in the rehabilitation of balance disorders and can be adapted to patients according to their needs. We present an experimental study that has been carried out using the system. The aim of the study is to determine whether this setup could be applied as a Virtual Rehabilitation System for balance rehabilitation in Acquired Brain Injury. We randomly divided patients into two groups: a trial group and a control group. The trial group used eBaViR system during the rehabilitation sessions, and the control group followed traditional rehabilitation sessions. We obtained encouraging results. [ABSTRACT FROM AUTHOR]

El objetivo principal de este trabajo es replantear la posibilidad de realizar una síntesis entre la fenomenología de Husserl y la teoría crítica de la Escuela de Frankfurt. Para ello realizaremos una revisión crítica de los textos del primer Marcuse (1928-1933), cuyo proyecto filosófico consistió en formular una síntesis entre la ontología fenomenológica de Heidegger y el materialismo dialéctico de Marx. La tesis que defenderemos aquí es que este proyecto sigue siendo vigente, pero tomaremos como referente la fenomenología de Husserl, desde la que interpretaremos los textos de Marcuse, y no la ontología existencial de Heidegger.