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The tomato variety 'Provence' was used as the material, and the artificial light supplement test was carried out in the double-arch steel frame solar greenhouse, and T1 (2R3B, the ratio of red/blue light beads R:B=2:3), T2 (4R1B), T3 (6R1B), T4 (7R1B) four groups of red and blue light treatments were set up, and no artificial light was used as the control (CK) to explore the optimal red and blue light ratio for greenhouse tomato growth and photosynthetic response. The results showed that after 35 days of T1 treatment, the plant height, SPAD, transpiration rate (Tr) and photochemical quenching coefficient (qP) of tomato were significantly increased by 26.7%, 19.6%, 107.6% and 34.3%, respectively, compared with the control group. After 35 days of T3 treatment, the stem diameter, flower number, net photosynthetic rate (Pn), intercellular CO2 concentration (Ci), stomatal conductance (Gs), actual photochemical efficiency (ΦPSII) and electron transport rate (ETR) of tomato were significantly increased by 26.9%, 38.6%, 133.7%, 5.9%, 183.3%, 31.3% and 30.9%, respectively, compared with the control group. After 35 days of T4 treatment, the maximum photochemical efficiency (Fv/Fm) was significantly increased by 4.0% compared with the control group. Compared with the control group, the growth and photosynthesis of tomato could be significantly promoted under different red and blue light supplement treatments. Among the four red and blue combination light treatments of 2R3B, 4R1B, 6R1B and 7R1B in this experiment, the comprehensive effect of each index under 6R1B treatment was the best, which could provide some theoretical guidance for LED light supplement in greenhouse tomato cultivation. [ABSTRACT FROM AUTHOR]

The purpose of the study was to establish the characteristic atlas of Jingen injection and provide the basis for its quality evaluation. Chlorogenic acid was used as reference, and gradient elution was performed on Waters X Bridge C18 column (diameter 3.5 μm, 4.6 mm x 250 mm) with acetonitrile-0.1% phosphoric acid solution. The detection wavelength was 240 nm, the flow rate was 1.0 mL/min, and the column temperature was 30 °C. The results showed that there were 7 characteristic peaks in the chromatogram, and the retention time was consistent with that of the corresponding reference. The study indicates that the method has high precision, strong specificity and good repeatability, and can control the intrinsic quality of Jinjen injection more effectively. [ABSTRACT FROM AUTHOR]

Anaplasma (formerly Ehrlichia) phagocytophilum, Ehrlichia chaffeensis, and Neorickettsia (formerly Ehrlichia) sennetsu are intracellular vector-borne pathogens that cause human ehrlichiosis, an emerging infectious disease. We present the complete genome sequences of these organisms along with comparisons to other organisms in the Rickettsiales order. Ehrlichia spp. and Anaplasma spp. display a unique large expansion of immunodominant outer membrane proteins facilitating antigenic variation. All Rickettsiales have a diminished ability to synthesize amino acids compared to their closest free-living relatives. Unlike members of the Rickettsiaceae family, these pathogenic Anaplasmataceae are capable of making all major vitamins, cofactors, and nucleotides, which could confer a beneficial role in the invertebrate vector or the vertebrate host. Further analysis identified proteins potentially involved in vacuole confinement of the Anaplasmataceae, a life cycle involving a hematophagous vector, vertebrate pathogenesis, human pathogenesis, and lack of transovarial transmission. These discoveries provide significant insights into the biology of these obligate intracellular pathogens. © 2006 Dunning Hotopp.