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21 results on '"Nishimiya, Osamu"'

7. Hepatic estrogen-responsive genes relating to oogenesis in cutthroat trout (Oncorhynchus clarki): The transcriptional induction in primary cultured hepatocytes and the in vitro promoter transactivation in responses to estradiol-17β

Author

Nagata, Jun, primary, Mushirobira, Yuji, additional, Nishimiya, Osamu, additional, Yamaguchi, You, additional, Fujita, Toshiaki, additional, Hiramatsu, Naoshi, additional, Hara, Akihiko, additional, and Todo, Takashi, additional

Published
2021
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8. Changes in the Hepatic Expression of Three Vitellogenin Subtype Genes During Ovarian Development in Female White-Edged Rockfish (Sebastes taczanowskii)

Author

Nagata, Jun, primary, Wada, Satoru, additional, Nishimiya, Osamu, additional, Wu, Meiqin, additional, Mushirobira, Yuji, additional, Yamaguchi, Yo, additional, Yokono, Takanori, additional, Kawasaki, Takuma, additional, Matsubara, Takahiro, additional, Todo, Takashi, additional, Hara, Akihiko, additional, and Hiramatsu, Naoshi, additional

Published
2021
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9. Quantitative Changes of Major Yolk Protein in the Coelomic Fluid and Gonads of the Sea Urchin, Mesocentrotus nudus, during the Reproductive Cycle

Author

Ura, Kazuhiro, Suzuki, Natsuki, Takei, Narumi, Higuchi, Ichiro, Yuhi, Tomoharu, Nishimiya, Osamu, and Takagi, Yasuaki

Subjects
Sea urchin, Reproductive cycle, Mesocentrotus nudus, Coelomic fluid, Gonad, Major yolk protein
Abstract

Both female and male sea urchins accumulate the major yolk protein (MYP) in the nutritive phagocytes of immature gonads before gametogenesis. Additionally, the most abundant protein in the coelomic fluids of both sexes is MYP. In females, MYP in the coelomic fluid is taken up by the nutritive phagocytes and then transported into growing oocytes. In this study, quantitative changes of MYP in the coelomic fluid of both sexes were examined during the reproductive cycle of the wild sea urchin, Mesocentrotus nudus. In females but not males, positive correlation between MYP level and the gonadosomatic index was observed. These results indicate that MYP in the coelomic fluid is suitable as a biomarker of the onset of puberty and progression of maturity in female sea urchins.

Published
2019

12. 北海道南部におけるキタムラサキウニ（Mesocentrotus nudus）の生殖周期と主要卵黄タンパク質 mRNA 量

Author

Ura, Kazuhiro, Wang, Heng, Hori, Tetsuo, Aizawa, Shunsuke, Tsue, Shiori, Satoh, Mizuha, Takei, Narumi, Hoshino, Kosuke, Higuchi, Ichiro, Sanuki, Shun, Yuhi, Tomoharu, Nishimiya, Osamu, and Takagi, Yasuaki

Subjects
Reproductive cycle, Sea urchin, Mesocentrotus nudus, Gonads, Yolk protein
Abstract

We examined the reproductive cycle and transcription-level changes of the major yolk protein (MYP) in gonads of both sexes during the different reproductive stages of wild northern sea urchin, Mesocentrotus nudus, collected in two areas of southern Hokkaido, Japan. Histological evaluation revealed mature gonads in both April and August, suggesting two annual spawning seasons at Otoshibe, whereas one annual spawning season was observed in autumn (September) at Usujiri. The transcription levels of MYP, which is synthesized and stored in the gonads, increased from stage 1 (recovering) to stage 2 (growth) and thereafter decreased, in both female and male sea urchins. The current results indicate that two annual spawning seasons may occur in wild populations of the northern sea urchin in southern Hokkaido.

Published
2017

17. Ovarian yolk formation in fishes: Molecular mechanisms underlying formation of lipid droplets and vitellogenin-derived yolk proteins

Author

Hiramatsu, Naoshi, primary, Todo, Takashi, additional, Sullivan, Craig V., additional, Schilling, Justin, additional, Reading, Benjamin J., additional, Matsubara, Takahiro, additional, Ryu, Yong-Woon, additional, Mizuta, Hiroko, additional, Luo, Wenshu, additional, Nishimiya, Osamu, additional, Wu, Meiqin, additional, Mushirobira, Yuji, additional, Yilmaz, Ozlem, additional, and Hara, Akihiko, additional

Published
2015
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21. Hepatic estrogen-responsive genes relating to oogenesis in cutthroat trout (Oncorhynchus clarki): The transcriptional induction in primary cultured hepatocytes and the in vitro promoter transactivation in responses to estradiol-17β.

Author

Nagata, Jun, Mushirobira, Yuji, Nishimiya, Osamu, Yamaguchi, You, Fujita, Toshiaki, Hiramatsu, Naoshi, Hara, Akihiko, and Todo, Takashi

Subjects
*OVUM physiology, *PROTEIN metabolism, *IN vitro studies, *CELL culture, *ESTRADIOL, *CELL receptors, *GENE expression, *MESSENGER RNA, *DOSE-effect relationship in pharmacology, *DESCRIPTIVE statistics, *LIVER cells
Abstract

• Choriogenin, vitellogenin and estrogen receptor-1a genes were estrogen-responsive. • These estrogen responsive genes responded differentially to estradiol-17β (E2). • E2 transactivated these gene promoters in the presence of four estrogen receptors. Estradiol-17β (E2) regulates transcription of estrogen-responsive genes via estrogen receptors (Esr). In many teleost species, choriogenin (chg), vitellogenin (vtg) and esr genes are transactivated by E2 in the liver. This study aimed i) to compare expression properties of all subtypes of these genes (chg : chgHα , chgHβ , chgL ; vtg : vtgAs , vtgC ; esr : esr1a , esr1b , esr2a , esr2b) in response to estrogen stimulation, and ii) to confirm how each of four Esr subtypes is involved in the transcriptional regulation of these estrogen-responsive genes in cutthroat trout hepatocytes. In hepatocytes in primary culture, all chg and vtg subtype mRNA levels, and those of esr1a , were increased by E2 treatment (10−6 M) at 24 and 72 h post initiation (hpi), but esr1b , esr2a and esr2b mRNA levels were not. Treatment of hepatocytes with various concentrations of E2 (10−11–10−6 M) induced dose-dependent increases in the levels of all chg and vtg subtype mRNAs at 24 and 72 hpi. At both time points, the lowest dose that induced a significant increase in the expression levels of mRNAs (LOEC) for E2 differed among the genes; LOECs were estimated as 10−11 M for chgHα at 24 hpi, as 10−9 M for vtgC at 72 hpi, and as 10−10 M for other mRNAs at both 24 and 72 hpi. Meanwhile, the levels of esr1a mRNA exhibited a dose-dependent increase at 24 and 72 hpi, but the LOEC shifted from 10−9 M at 24 hpi to 10−7 M at 72 hpi because of a decrease in mRNA levels at treatment groups exposed to high concentrations of E2. All Esr subtypes transactivated chg , vtg and esr1a promoters in the presence of E2 in vitro. The activation levels indicated that promoter activity of chgHα ≥ vtgAs > chgHβ > chgL ≥ vtgC ≥ esr1a when mediated by Esr1a, chgHβ > chgHα > chgHL > vtgAs ≥ vtgC ≥ esr1a by Esr1b, chgHβ ≥ chgL > chgHα ≥ vtgAs > vtgC > esr1a by Esr2a, and chgHβ ≥ chgHα ≥ vtgAs > chgL ≥ vtgC > esr1a by Esr2b. Collectively, different Esr subtypes were distinctly different in their ability to transactivate estrogen-responsive target genes, resulting in differential expression of chg , vtg and esr1a genes in the estrogen-exposed hepatocytes. [ABSTRACT FROM AUTHOR]

Published
2021
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