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1. Proteomic profiling of recombinant Escherichia coli in high-cell-density fermentations for improved production of an antibody fragment biopharmaceutical

Author

Aldor, Ilana S., Forrest, William, Krawitz, Denise C., Christina Chen, Champion, Kathleen M., Nishihara, Julie C., and Joly, John C.

Subjects
Gel electrophoresis -- Analysis, Polyacrylamide -- Analysis, Proteomics -- Analysis, Escherichia coli -- Analysis, Biological sciences
Abstract

An analysis of a proteomic is conducted over time with high-cell-density, industrial, phosphate-limited Escherichia coli fermentations at the 10-litre scale using two-dimensional polyacrylamide gel electrophoresis. E.coli protein changes associated with culture conditions were distinguished from protein changes associated with heterologous protein expression.

Published
2005

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