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2. Genetic tool development in marine protists: emerging model organisms for experimental cell biology

Author

Faktorová, Drahomíra, Nisbet, R Ellen R, Fernández Robledo, José A, Casacuberta, Elena, Sudek, Lisa, Allen, Andrew E, Ares, Manuel, Aresté, Cristina, Balestreri, Cecilia, Barbrook, Adrian C, Beardslee, Patrick, Bender, Sara, Booth, David S, Bouget, François-Yves, Bowler, Chris, Breglia, Susana A, Brownlee, Colin, Burger, Gertraud, Cerutti, Heriberto, Cesaroni, Rachele, Chiurillo, Miguel A, Clemente, Thomas, Coles, Duncan B, Collier, Jackie L, Cooney, Elizabeth C, Coyne, Kathryn, Docampo, Roberto, Dupont, Christopher L, Edgcomb, Virginia, Einarsson, Elin, Elustondo, Pía A, Federici, Fernan, Freire-Beneitez, Veronica, Freyria, Nastasia J, Fukuda, Kodai, García, Paulo A, Girguis, Peter R, Gomaa, Fatma, Gornik, Sebastian G, Guo, Jian, Hampl, Vladimír, Hanawa, Yutaka, Haro-Contreras, Esteban R, Hehenberger, Elisabeth, Highfield, Andrea, Hirakawa, Yoshihisa, Hopes, Amanda, Howe, Christopher J, Hu, Ian, Ibañez, Jorge, Irwin, Nicholas AT, Ishii, Yuu, Janowicz, Natalia Ewa, Jones, Adam C, Kachale, Ambar, Fujimura-Kamada, Konomi, Kaur, Binnypreet, Kaye, Jonathan Z, Kazana, Eleanna, Keeling, Patrick J, King, Nicole, Klobutcher, Lawrence A, Lander, Noelia, Lassadi, Imen, Li, Zhuhong, Lin, Senjie, Lozano, Jean-Claude, Luan, Fulei, Maruyama, Shinichiro, Matute, Tamara, Miceli, Cristina, Minagawa, Jun, Moosburner, Mark, Najle, Sebastián R, Nanjappa, Deepak, Nimmo, Isabel C, Noble, Luke, Novák Vanclová, Anna MG, Nowacki, Mariusz, Nuñez, Isaac, Pain, Arnab, Piersanti, Angela, Pucciarelli, Sandra, Pyrih, Jan, Rest, Joshua S, Rius, Mariana, Robertson, Deborah, Ruaud, Albane, Ruiz-Trillo, Iñaki, Sigg, Monika A, Silver, Pamela A, Slamovits, Claudio H, Jason Smith, G, Sprecher, Brittany N, Stern, Rowena, Swart, Estienne C, Tsaousis, Anastasios D, Tsypin, Lev, Turkewitz, Aaron, and Turnšek, Jernej

Subjects
Genetics, Biotechnology, Life Below Water, Biodiversity, DNA, Ecosystem, Environment, Eukaryota, Green Fluorescent Proteins, Marine Biology, Models, Biological, Species Specificity, Transformation, Genetic, Biological Sciences, Technology, Medical and Health Sciences, Developmental Biology
Abstract

Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.

Published
2020

3. Publisher Correction: Genetic tool development in marine protists: emerging model organisms for experimental cell biology

Author

Faktorová, Drahomíra, Nisbet, R Ellen R, Fernández Robledo, José A, Casacuberta, Elena, Sudek, Lisa, Allen, Andrew E, Ares, Manuel, Aresté, Cristina, Balestreri, Cecilia, Barbrook, Adrian C, Beardslee, Patrick, Bender, Sara, Booth, David S, Bouget, François-Yves, Bowler, Chris, Breglia, Susana A, Brownlee, Colin, Burger, Gertraud, Cerutti, Heriberto, Cesaroni, Rachele, Chiurillo, Miguel A, Clemente, Thomas, Coles, Duncan B, Collier, Jackie L, Cooney, Elizabeth C, Coyne, Kathryn, Docampo, Roberto, Dupont, Christopher L, Edgcomb, Virginia, Einarsson, Elin, Elustondo, Pía A, Federici, Fernan, Freire-Beneitez, Veronica, Freyria, Nastasia J, Fukuda, Kodai, García, Paulo A, Girguis, Peter R, Gomaa, Fatma, Gornik, Sebastian G, Guo, Jian, Hampl, Vladimír, Hanawa, Yutaka, Haro-Contreras, Esteban R, Hehenberger, Elisabeth, Highfield, Andrea, Hirakawa, Yoshihisa, Hopes, Amanda, Howe, Christopher J, Hu, Ian, Ibañez, Jorge, Irwin, Nicholas AT, Ishii, Yuu, Janowicz, Natalia Ewa, Jones, Adam C, Kachale, Ambar, Fujimura-Kamada, Konomi, Kaur, Binnypreet, Kaye, Jonathan Z, Kazana, Eleanna, Keeling, Patrick J, King, Nicole, Klobutcher, Lawrence A, Lander, Noelia, Lassadi, Imen, Li, Zhuhong, Lin, Senjie, Lozano, Jean-Claude, Luan, Fulei, Maruyama, Shinichiro, Matute, Tamara, Miceli, Cristina, Minagawa, Jun, Moosburner, Mark, Najle, Sebastián R, Nanjappa, Deepak, Nimmo, Isabel C, Noble, Luke, Novák Vanclová, Anna MG, Nowacki, Mariusz, Nuñez, Isaac, Pain, Arnab, Piersanti, Angela, Pucciarelli, Sandra, Pyrih, Jan, Rest, Joshua S, Rius, Mariana, Robertson, Deborah, Ruaud, Albane, Ruiz-Trillo, Iñaki, Sigg, Monika A, Silver, Pamela A, Slamovits, Claudio H, Jason Smith, G, Sprecher, Brittany N, Stern, Rowena, Swart, Estienne C, Tsaousis, Anastasios D, Tsypin, Lev, Turkewitz, Aaron, and Turnšek, Jernej

Subjects
Biological Sciences, Technology, Medical and Health Sciences, Developmental Biology
Abstract

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Published
2020

8. Persistence, spread and the drift paradox

Author

Pachepsky, E, Lutscher, F, Nisbet, R M, and Lewis, M A

Subjects
drift paradox, stream, aquatic insects, propagation speed, critical domain size, persistence
Abstract

We derive conditions for persistence and spread of a population where individuals are either immobile or dispersing by advection and diffusion through a one-dimensional medium with a unidirectional flow. Reproduction occurs only in the stationary phase. Examples of such systems are found in rivers and streams, marine currents, and areas with prevalent wind direction. In streams, a long-standing question, dubbed 'the drift paradox', asks why aquatic insects faced with downstream drift are able to persist in upper stream reaches. For our two-phase model, persistence of the population is guaranteed if, at low population densities, the local growth rate of the stationary component of the population exceeds the rate of entry of individuals into the drift. Otherwise the persistence condition involves all the model parameters, and persistence requires a critical (minimum) domain size. We calculate the rate at which invasion fronts propagate up- and downstream, and show that persistence and ability to spread are closely connected: if the population cannot advance upstream against the flow, it also cannot persist on any finite spatial domain. By studying two limiting cases of our model, we show that residence in the immobile state always enhances population persistence. We use our findings to evaluate a number of mechanisms previously proposed in the ecological literature as resolutions of the drift paradox. (C) 2004 Elsevier Inc. All rights reserved.

Published
2005

18. Evolution: The plasticity of plastids.

Author

Howe, Christopher J., Nisbet, R. Ellen R., and Barbrook, Adrian C.

Subjects
*PLASTIDS, *CHLOROPLASTS, *ALGAE, *MACHINERY
Abstract

Many chloroplast-bearing plants and algae lost their photosynthetic activity during evolution but retained their chloroplasts for other functions. A group of dinoflagellate algae apparently lost one half of their photosynthetic machinery but retained the other, providing a novel mechanism for light perception. [ABSTRACT FROM AUTHOR]

Published
2023
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25. An essential pentatricopeptide repeat protein in the apicomplexan remnant chloroplast

Author

Hicks, Joanna L, Lassadi, Imen, Carpenter, Emma F, Eno, Madeleine, Vardakis, Alexandros, Waller, Ross F, Howe, Christopher J, Nisbet, R Ellen R, Nisbet, Ellen, Carpenter, Emma F [0000-0002-1911-6842], Waller, Ross F [0000-0001-6961-9344], Howe, Christopher J [0000-0002-6975-8640], Nisbet, R Ellen R [0000-0003-4487-196X], and Apollo - University of Cambridge Repository

Subjects
Plasmodium, apicoplast, Chloroplasts, Plasmodium falciparum, Immunology, food and beverages, Apicoplasts, Microbiology, RNA processing, apicomplexa, Virology, parasitic diseases, plastid, Toxoplasma, Phylogeny
Abstract

The malaria parasite Plasmodium and other apicomplexans such as Toxoplasma evolved from photosynthetic organisms and contain an essential, remnant plastid termed the apicoplast. Transcription of the apicoplast genome is polycistronic with extensive RNA processing. Yet little is known about the mechanism of apicoplast RNA processing. In plants, chloroplast RNA processing is controlled by multiple pentatricopeptide repeat (PPR) proteins. Here, we identify the single apicoplast PPR protein, PPR1. We show that the protein is essential and that it binds to RNA motifs corresponding with previously characterized processing sites. Additionally, PPR1 shields RNA transcripts from ribonuclease degradation. This is the first characterization of a PPR protein from a nonphotosynthetic plastid.

Published
2019

46. The botanical record of archaeobotany Italian network - Brain: A cooperative network, database and website*

Author

Lippi, M, Florenzano, A, Rinaldi, R, Allevato, E, Arobba, D, Bacchetta, G, Bal, M, Mazzanti, M, Benatti, A, Benes, J, Bosi, G, Buonincontri, M, Caramiello, R, Castelletti, L, Castiglioni, E, Celant, A, Clo, E, Costantini, L, Pasquale, G, Di Rita, F, Fiorentino, G, Furlanetto, G, Giardini, M, Grillo, O, Guido, M, Herchenbach, M, Magri, D, Marchesini, M, Maritan, M, Marvelli, S, Masi, A, Miola, A, Montanari, C, Montecchi, M, Motella, S, Nisbet, R, Orru, M, Pena-Chocarro, L, Pepe, C, Perego, R, Rattighieri, E, Ravazzi, C, Rottoli, M, Rowan, E, Sabato, D, Sadori, L, Sarigu, M, Torri, P, Ucchesu, M, Mercuri, A, Lippi M. M., Florenzano A., Rinaldi R., Allevato E., Arobba D., Bacchetta G., Bal M. -C., Mazzanti M. B., Benatti A., Benes J., Bosi G., Buonincontri M., Caramiello R., Castelletti L., Castiglioni E., Celant A., Clo E., Costantini L., Pasquale G. D., Di Rita F., Fiorentino G., Furlanetto G., Giardini M., Grillo O., Guido M., Herchenbach M., Magri D., Marchesini M., Maritan M., Marvelli S., Masi A., Miola A., Montanari C., Montecchi M. C., Motella S., Nisbet R., Orru M., Pena-Chocarro L., Pepe C., Perego R., Rattighieri E., Ravazzi C., Rottoli M., Rowan E., Sabato D., Sadori L., Sarigu M., Torri P., Ucchesu M., Mercuri A. M., Lippi, M, Florenzano, A, Rinaldi, R, Allevato, E, Arobba, D, Bacchetta, G, Bal, M, Mazzanti, M, Benatti, A, Benes, J, Bosi, G, Buonincontri, M, Caramiello, R, Castelletti, L, Castiglioni, E, Celant, A, Clo, E, Costantini, L, Pasquale, G, Di Rita, F, Fiorentino, G, Furlanetto, G, Giardini, M, Grillo, O, Guido, M, Herchenbach, M, Magri, D, Marchesini, M, Maritan, M, Marvelli, S, Masi, A, Miola, A, Montanari, C, Montecchi, M, Motella, S, Nisbet, R, Orru, M, Pena-Chocarro, L, Pepe, C, Perego, R, Rattighieri, E, Ravazzi, C, Rottoli, M, Rowan, E, Sabato, D, Sadori, L, Sarigu, M, Torri, P, Ucchesu, M, Mercuri, A, Lippi M. M., Florenzano A., Rinaldi R., Allevato E., Arobba D., Bacchetta G., Bal M. -C., Mazzanti M. B., Benatti A., Benes J., Bosi G., Buonincontri M., Caramiello R., Castelletti L., Castiglioni E., Celant A., Clo E., Costantini L., Pasquale G. D., Di Rita F., Fiorentino G., Furlanetto G., Giardini M., Grillo O., Guido M., Herchenbach M., Magri D., Marchesini M., Maritan M., Marvelli S., Masi A., Miola A., Montanari C., Montecchi M. C., Motella S., Nisbet R., Orru M., Pena-Chocarro L., Pepe C., Perego R., Rattighieri E., Ravazzi C., Rottoli M., Rowan E., Sabato D., Sadori L., Sarigu M., Torri P., Ucchesu M., and Mercuri A. M.

Abstract

The BRAIN (Botanical Records of Archaeobotany Italian Network) database and network was developed by the cooperation of archaeobotanists working on Italian archaeological sites. Examples of recent research including pollen or other plant remains in analytical and synthetic papers are reported as an exemplar reference list. This paper retraces the main steps of the creation of BRAIN, from the scientific need for the first research cooperation to the website which has a free online access since 2015.

Published
2018

47. New AMS 14C dates track the arrival and spread of broomcorn millet cultivation and agricultural change in prehistoric Europe

Author

Filipovic, D., Meadows, J., Corso, M. D., Kirleis, W., Alsleben, A., Akeret, O., Bittmann, F., Bosi, G., Ciuta, B., Dreslerova, D., Effenberger, H., Gyulai, F., Heiss, A. G., Hellmund, M., Jahns, S., Jakobitsch, T., Kapcia, M., Klooss, S., Kohler-Schneider, M., Kroll, H., Makarowicz, P., Marinova, E., Markle, T., Medovic, A., Mercuri, A. M., Mueller-Bieniek, A., Nisbet, R., Pashkevich, G., Perego, R., Pokorny, P., Pospieszny, L., Przybyla, M., Reed, K., Rennwanz, J., Stika, H. -P., Stobbe, A., Tolar, T., Wasylikowa, K., Wiethold, J., Zerl, T., Institut fûr Ur- und Frühgeschichte, Christian-Albrechts-Universität zu Kiel (CAU), Centre for Baltic and Scandinavian Archaeology (ZBSA), Schleswig-Holstein State Museums Foundation (ZBSA), Centre for Baltic and Scandinavian Archaeology (ZBSA), Integrative Prähistorische und Naturwissenschaftliche Archäologie, Basel, Niedersächsisches Institut für Historische Küstenforschung (NIHK), Dipartimento di Scienze della Vita, Università degli Studi di Modena e Reggio EmiliaUniversità degli Studi di Modena e Reggio Emilia, Facultatea de Istorie şi Filologie, Universitatea '1 Decembrie 1918' Alba Iulia, Institute of Archaeology of the Czech Academy of Sciences, Prague, Austrian Archaeological Institute (ÖAI), Austrian Archaeological Institute (ÖAI), Austrian Academy of Sciences (ÖAI), Landesamt für Denkmalpflege und Archäologie Sachsen-Anhalt (LDA-LSA), Brandenburgisches Landesamt für Denkmalpflege und Archäologisches Landesmuseum, Archäologisches Landesamt Schleswig-Holstein, Faculty of Archaeology, Adam Mickiewicz University, Poznan, Landesamt für Denkmalpflege Baden-Württemberg, Museum of Vojvodina, Partenaires INRAE, Department of Palaeobotany, W. Szafer Institute of Botany, Polish Academy of Sciences, Archéologie, Terre, Histoire, Sociétés [Dijon] (ARTeHiS), Ministère de la Culture et de la Communication (MCC)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Institut national de recherches archéologiques préventives (Inrap), Institut fur UR-und Frühgeschichte, and Universität zu Köln

Subjects
Europe, Environmental social sciences, Science, Panicum, Archaeobotany, Europe, Neolithic, AMS dating, Medicine, Archaeobotany, Neolithic, Panicum, AMS dating, Plant sciences, [SHS]Humanities and Social Sciences
Abstract

International audience; Broomcorn millet (Panicum miliaceum L.) is not one of the founder crops domesticated in Southwest Asia in the early Holocene, but was domesticated in northeast China by 6000 BC. In Europe, millet was reported in Early Neolithic contexts formed by 6000 BC, but recent radiocarbon dating of a dozen 'early' grains cast doubt on these claims. Archaeobotanical evidence reveals that millet was common in Europe from the 2nd millennium BC, when major societal and economic transformations took place in the Bronze Age. We conducted an extensive programme of AMS-dating of charred broomcorn millet grains from 75 prehistoric sites in Europe. Our Bayesian model reveals that millet cultivation began in Europe at the earliest during the sixteenth century BC, and spread rapidly during the fifteenth/fourteenth centuries BC. Broomcorn millet succeeds in exceptionally wide range of growing conditions and completes its lifecycle in less than three summer months. Offering an additional harvest and thus surplus food/fodder, it likely was a transformative innovation in European prehistoric agriculture previously based mainly on (winter) cropping of wheat and barley. We provide a new, high-resolution chronological framework for this key agricultural development that likely contributed to far-reaching changes in lifestyle in late 2nd millennium BC Europe.

Published
2020

48. Genetic tool development in marine protists: emerging model organisms for experimental cell biology

Author

Nisbet, R. Ellen R., Casacuberta, Elena, Sudek, Lisa, Allen, Andrew E., Ares, Manuel, Balestreri, Cecilia, Barbrook, Adrian C., Beardslee, Patrick, Bender, Sara, Booth, David S., Bowler, Chris, Breglia, Susana A., Brownlee, Colin, Burger, Gertraud, Cerutti, Heriberto, Cesaroni, Rachele, Chiurillo, Miguel A., Clemente, Thomas, Coles, Duncan B., Collier, Jackie L., Cooney, Elizabeth C., Coyne, Kathryn, Docampo, Roberto, Dupont, Christopher L., Edgcomb, Virginia, Einarsson, Elin, Federici, Fernan, Freire-Beneitez, Veronica, Freyria, Nastasia J., Fukuda, Kodai, Girguis, Peter R., Gomaa, Fatma, Gornik, Sebastian G., Guo, Jian, Hanawa, Yutaka, Haro-Contreras, Esteban R., Hehenberger, Elisabeth, Highfield, Andrea, Hirakawa, Yoshihisa, Hopes, Amanda, Howe, Christopher J., Hu, Ian, Irwin, Nicholas A. T., Ishii, Yuu, Janowicz, Natalia Ewa, Jones, Adam C., Kachale, Ambar, Fujimura-Kamada, Konomi, Kaur, Binnypreet, Kaye, Jonathan Z., Kazana, Eleanna, Keeling, Patrick J., King, Nicole, Klobutcher, Lawrence A., Lander, Noelia, Lassadi, Imen, Li, Zhuhong, Lin, Senjie, Lozano, Jean-Claude, Luan, Fulei, Maruyama, Shinichiro, Matute, Tamara, Miceli, Cristina, Minagawa, Jun, Moosburner, Mark, Nanjappa, Deepak, Nimmo, Isabel C., Noble, Luke, Nowacki, Mariusz, Pain, Arnab, Piersanti, Angela, Pucciarelli, Sandra, Pyrih, Jan, Rest, Joshua S., Rius, Mariana, Robertson, Deborah, Ruaud, Albane, Sigg, Monika A., Silver, Pamela A., Slamovits, Claudio H., Jason Smith, G., Sprecher, Brittany N., Stern, Rowena, Swart, Estienne C., Tsaousis, Anastasios D., Tsypin, Lev, Turkewitz, Aaron, Valach, Matus, von Dassow, Peter, von der Haar, Tobias, Waller, Ross F., Wang, Lu, Wen, Xiaoxue, Wheeler, Glen, Woods, April, Zhang, Huan, Mock, Thomas, and Worden, Alexandra Z.

Subjects
Cell Biology, Biochemistry, Molecular Biology, Biotechnology
Abstract

Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.

Published
2020

49. Genetic tool development in marine protists: emerging model organisms for experimental cell biology

Author

Gordon and Betty Moore Foundation, Leverhulme Trust, Ministry of Education, Youth and Sports (Czech Republic), European Commission, Faktorová, Drahomíra, Nisbet, R. Ellen R, Robledo, José A. F., Casacuberta, Elena, Sudek, Lisa, Allen, Andrew E., Ares, Manuel, Aresté, Cristina, Balestreri, Cecilia, Barbrook, Adrian C., Beardslee, Patrick, Ruaud, Albane, Ruiz-Trillo, Iñaki, Sigg, Monika A., Silver, Pamela A., Slamovits, Claudio H., Smith, G. Jason, Sprecher, Brittany N., Stern, Rowena, Swart, Estienne C., Tsaousis, Anastasios D., Valach, Matus, Tsypin, Lev, Turkewitz, Aaron, Turnšek, Jernej, Vergé, Valérie, von Dassow, Peter, von der Haar, Tobias, Waller, Ross F., Wang, Lu, Wen, Xiaoxue, Wheeler, Glen, Woods, April, Zhang, Huan, Bender, Sara, Mock, Thomas, Worden, Alexandra Z., Lukeš, Julius, Booth, David S., Bouget, François-Yves, Bowler, Chris, Breglia, Susana A., Brownlee, Colin, Burger, Gertraud, Cerutti, Heriberto, Cesaroni, Rachele, Chiurillo, Miguel A., Clemente, Thomas, Coles, Duncan B., Collier, Jackie L., Cooney, Elizabeth C., Coyne, Kathryn, Docampo, Roberto, Dupont, Christopher L., Edgcomb, Virginia, Einarsson, Elin, Elustondo, Pía A., Federici, Fernan, Freire-Beneitez, Verónica, Freyria, Nastasia J., Fukuda, Kodai, García, Paulo A., Girguis, Peter R., Gomaa, Fatma, Gornik, Sebastian G., Guo, Jian, Hampl, Vladimir, Hanawa, Yutaka, Haro-Contreras, Esteban R., Hehenberger, Elisabeth, Highfield, Andrea, Hirakawa, Yoshihisa, Hopes, Amanda, Howe, Christopher J., Hu, Ian, Ibañez, Jorge, Irwin, Nicholas A. T., Ishii, Yuu, Janowicz, Natalia Ewa, Jones, Adam C., Kachale, Ambar, Fujimura-Kamada, Konomi, Kaur, Binnypreet, Kaye, Jonathan Z., Kazana, Eleanna, Keeling, Patrick J., King, Nicole, Klobutcher, Lawrence A., Lander, Noelia, Lassadi, Imen, Li, Zhuhong, Lin, Senjie, Lozano, Jean-Claude, Luan, Fulei, Maruyama, Shinichiro, Matute, Tamara, Miceli, Cristina, Minagawa, Jun, Moosburner, Mark, Najle, Sebastián R., Nanjappa, Deepak, Nimmo, Isabel C., Noble, Luke, Novák Vanclová, Anna M. G., Nowacki, Mariusz, Núñez, Isaac, Pain, Arnab, Piersanti, Angela, Pucciarelli, Sandra, Pyrih, Jan, Rest, Joshua S., Rius, Mariana, Robertson, Deborah, Gordon and Betty Moore Foundation, Leverhulme Trust, Ministry of Education, Youth and Sports (Czech Republic), European Commission, Faktorová, Drahomíra, Nisbet, R. Ellen R, Robledo, José A. F., Casacuberta, Elena, Sudek, Lisa, Allen, Andrew E., Ares, Manuel, Aresté, Cristina, Balestreri, Cecilia, Barbrook, Adrian C., Beardslee, Patrick, Ruaud, Albane, Ruiz-Trillo, Iñaki, Sigg, Monika A., Silver, Pamela A., Slamovits, Claudio H., Smith, G. Jason, Sprecher, Brittany N., Stern, Rowena, Swart, Estienne C., Tsaousis, Anastasios D., Valach, Matus, Tsypin, Lev, Turkewitz, Aaron, Turnšek, Jernej, Vergé, Valérie, von Dassow, Peter, von der Haar, Tobias, Waller, Ross F., Wang, Lu, Wen, Xiaoxue, Wheeler, Glen, Woods, April, Zhang, Huan, Bender, Sara, Mock, Thomas, Worden, Alexandra Z., Lukeš, Julius, Booth, David S., Bouget, François-Yves, Bowler, Chris, Breglia, Susana A., Brownlee, Colin, Burger, Gertraud, Cerutti, Heriberto, Cesaroni, Rachele, Chiurillo, Miguel A., Clemente, Thomas, Coles, Duncan B., Collier, Jackie L., Cooney, Elizabeth C., Coyne, Kathryn, Docampo, Roberto, Dupont, Christopher L., Edgcomb, Virginia, Einarsson, Elin, Elustondo, Pía A., Federici, Fernan, Freire-Beneitez, Verónica, Freyria, Nastasia J., Fukuda, Kodai, García, Paulo A., Girguis, Peter R., Gomaa, Fatma, Gornik, Sebastian G., Guo, Jian, Hampl, Vladimir, Hanawa, Yutaka, Haro-Contreras, Esteban R., Hehenberger, Elisabeth, Highfield, Andrea, Hirakawa, Yoshihisa, Hopes, Amanda, Howe, Christopher J., Hu, Ian, Ibañez, Jorge, Irwin, Nicholas A. T., Ishii, Yuu, Janowicz, Natalia Ewa, Jones, Adam C., Kachale, Ambar, Fujimura-Kamada, Konomi, Kaur, Binnypreet, Kaye, Jonathan Z., Kazana, Eleanna, Keeling, Patrick J., King, Nicole, Klobutcher, Lawrence A., Lander, Noelia, Lassadi, Imen, Li, Zhuhong, Lin, Senjie, Lozano, Jean-Claude, Luan, Fulei, Maruyama, Shinichiro, Matute, Tamara, Miceli, Cristina, Minagawa, Jun, Moosburner, Mark, Najle, Sebastián R., Nanjappa, Deepak, Nimmo, Isabel C., Noble, Luke, Novák Vanclová, Anna M. G., Nowacki, Mariusz, Núñez, Isaac, Pain, Arnab, Piersanti, Angela, Pucciarelli, Sandra, Pyrih, Jan, Rest, Joshua S., Rius, Mariana, and Robertson, Deborah

Abstract

Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.

Published
2020

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