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C20H18F2N2, monoclinic, P21/c (no. 14), a = 12.4233(11) Å, b = 7.3805(7) Å, c = 18.9531(16) Å, β = 104.109(3)°, V = 104.109(3) Å3, Z = 4, Rgt(F) = 0.0464, wRref(F2) = 0.1201, T = 296(2) K.

Given the wide application of hybrid engines, how to improve hybrid engine fuel economy is being more and more studied, and using the Atkinson cycle to improve fuel economy is considered effective. In this study, the in-cylinder direct injection engine model was established with the data obtained from a benchmarking test using the GT-POWER simulation software. The working process of this engine was simulated after using Atkinson cycle. This simulation primarily focused on the research of the impact on engine fuel economy with different late intake valve closing strategies. The simulation results were calculated under the partial load conditions which are typically used in hybrid engines. The results indicated that engine fuel economy improved and fuel consumption decreased by using the Atkinson cycle. However, the Atkinson cycle would cause a decrease in power.

Anaplasma (formerly Ehrlichia) phagocytophilum, Ehrlichia chaffeensis, and Neorickettsia (formerly Ehrlichia) sennetsu are intracellular vector-borne pathogens that cause human ehrlichiosis, an emerging infectious disease. We present the complete genome sequences of these organisms along with comparisons to other organisms in the Rickettsiales order. Ehrlichia spp. and Anaplasma spp. display a unique large expansion of immunodominant outer membrane proteins facilitating antigenic variation. All Rickettsiales have a diminished ability to synthesize amino acids compared to their closest free-living relatives. Unlike members of the Rickettsiaceae family, these pathogenic Anaplasmataceae are capable of making all major vitamins, cofactors, and nucleotides, which could confer a beneficial role in the invertebrate vector or the vertebrate host. Further analysis identified proteins potentially involved in vacuole confinement of the Anaplasmataceae, a life cycle involving a hematophagous vector, vertebrate pathogenesis, human pathogenesis, and lack of transovarial transmission. These discoveries provide significant insights into the biology of these obligate intracellular pathogens.

Abstract Background Epidemiological evidence supports the association between exposure to ambient particulate matter (PM) and cardiovascular diseases. Chronic exposure to ultrafine particles (UFP; Dp Results UFP2 contained a higher level of redox active organic compounds and metals on a per PM mass basis than UFP1. While both UFP1 and UFP2 induced superoxide production and up-regulated stress response genes such as heme oxygenease-1 (HO-1), OKL38, and tissue factor (TF), only UFP2 induced the expression of pro-inflammatory genes such as IL-8 (2.8 ± 0.3-fold), MCP-1 (3.9 ± 0.4-fold), and VCAM (6.5 ± 1.1-fold) (n = 3, P < 0.05). UFP2-exposed HAEC also bound to a higher number of monocytes than UFP1-exposed HAEC (Control = 70 ± 7.5, UFP1 = 106.7 ± 12.5, UFP2 = 137.0 ± 8.0, n = 3, P < 0.05). Adenovirus NF-κB Luciferase reporter assays revealed that UFP2, but not UFP1, significantly induced NF-κB activities. NF-κB inhibitor, CAY10512, significantly abrogated UFP2-induced pro-inflammatory gene expression and monocyte binding. Conclusion While UFP1 induced higher level of oxidative stress and stress response gene expression, only UFP2, with higher levels of redox active organic compounds and metals, induced pro-inflammatory responses via NF-κB signaling. Thus, UFP with distinct chemical compositions caused differential response patterns in endothelial cells.

Abstract Background A newly designed electrostatic precipitator (ESP) in tandem with Versatile Aerosol Concentration Enrichment System (VACES) was developed by the University of Southern California to collect ambient aerosols on substrates appropriate for chemical and toxicological analysis. The laboratory evaluation of this sampler is described in a previous paper. The main objective of this study was to evaluate the performance of the new VACES-ESP system in the field by comparing the chemical characteristics of the PM collected in the ESP to those of reference samplers operating in parallel. Results The field campaign was carried out in the period from August, 2007 to March, 2008 in a typical urban environment near downtown Los Angeles. Each sampling set was restricted to 2–3 hours to minimize possible sampling artifacts in the ESP. The results showed that particle penetration increases and ozone concentration decreases with increasing sampling flow rate, with highest particle penetration observed between 100 nm and 300 nm. A reference filter sampler was deployed in parallel to the ESP to collect concentration-enriched aerosols, and a MOUDI sampler was used to collect ambient aerosols. Chemical analysis results showed very good agreement between the ESP and MOUDI samplers in the concentrations of trace elements and inorganic ions. The overall organic compound content of PM collected by the ESP, including polycyclic aromatic hydrocarbons (PAHs), hopanes, steranes, and alkanes, was in good agreement with that of the reference sampler, with an average ESP -to -reference concentration ratio of 1.07 (± 0.38). While majority of organic compound ratios were close to 1, some of the semi-volatile organic species had slightly deviated ratios from 1, indicating the possibility of some sampling artifacts in the ESP due to reactions of PM with ozone and radicals generated from corona discharge, although positive and negative sampling artifacts in the reference filter sampler cannot be ruled out. Conclusion The very good overall agreement between ESP and reference samplers makes it an attractive alternative to filters and biosamplers for chemical and toxicological evaluation of PM properties, including the possibility of conducting direct in vitro cell exposures. Moreover, the concentration enrichment of ambient aerosols by the VACES allows for short-term exposure studies, which preserve cell viability and enable studies to PM generated from specific sources and-or formation mechanisms in the atmosphere.

BACKGROUND Hypertension is a risk factor for atrial fibrillation (AF), and brain and muscle arnt-like protein 1 (Bmal1) regulate circadian blood pressure and is implicated in several fibrotic disorders. Our hypothesis that Bmal1 inhibits atrial fibrosis and susceptibility to AF in salt-sensitive hypertension (SSHT) and our study provides a new target for the pathogenesis of AF induced by hypertension. METHODS The study involved 7-week-old male Dahl salt-sensitive that were fed either a high-salt diet (8% NaCl; DSH group) or a normal diet (0.3% NaCl; DSN group). An experimental model was used to measure systolic blood pressure (SBP), left atrial ejection fraction (LAEF), left atrial end-volume index (LAEVI), left atrial index (LAFI), AF inducibility, AF duration, and atrial fibrosis pathological examination and the expression of Baml1 and fibrosis-related proteins (TNF-α and α-SMA) in left atrial tissue. RESULTS DSH increased TNF-α and α-SMA expression in atrial tissue, level of SBP and LAESVI, atrial fibrosis, AF induction rate, and AF duration, and decreased Bmal1 expression in atrial tissue, the circadian rhythm of hypertension, and level of LAEF and LAFI. Our results also showed that the degree of atrial fibrosis was negatively correlated with Bmal1 expression, but positively correlated with the expression of TNF-α and α-SMA. CONCLUSIONS We demonstrated that a high-salt diet leads to circadian changes in hypertension due to a reduction of Bmal1 expression, which plays a crucial role in atrial fibrosis and increased susceptibility to AF in SSHT rats. [ABSTRACT FROM AUTHOR]

The rapid expansion of low-cost sensor networks for air quality monitoring necessitates rigorous calibration to ensure data accuracy. Despite numerous published field calibration studies, a universal and comprehensive assessment of factors affecting sensor calibration remains elusive, leading to potential discrepancies in data quality across different networks. To address these challenges, this study deployed eight sensor-based monitors equipped with electrochemical sensors for NO2, NO, CO, and O3 measurement in strategically chosen locations within Hong Kong, Macau, and Shanghai, covering a wide range of climatic conditions: Hong Kong's subtropical climate, Macau's similar yet distinct urban environment, and Shanghai's more variable climate. This strategic deployment ensured that the sensors' performance and calibration processes were tested across diverse atmospheric conditions. Each monitor employed a patented dynamic baseline tracking method for the gas sensors, which isolates the concentration signals from temperature and humidity effects, enhancing the sensors' accuracy and reliability. The tests, which involved evaluating the validation performance by analyzing randomly selected calibration sample subsets ranging from 1 to 15 days, indicated that the length of the calibration period, pollutant concentration range, and time averaging period are pivotal for sensor calibration quality. We determined that a 5–7 days calibration period minimizes calibration coefficient errors, and a wider concentration range improves the validation R2 values for all sensors, suggesting the necessity of setting specific concentration range thresholds. Moreover, a time averaging period of at least 5 minutes for data with 1-minute resolution was recommended to enable optimal calibration in field operation. This study emphasizes the need for a comprehensive calibration assessment and the importance of considering environmental variability in sensor calibration condition. These findings offer methodological guidance for the calibration of other sensor types, providing a reference for future research in the field of sensor calibration. [ABSTRACT FROM AUTHOR]

Pollution data were collected at five schools in Hong Kong using low-cost, sensor-based monitors both indoors and outdoors during two consecutive high pollution episodes. The pollutants monitored included NO2, O3, PM2.5, and PM10, which were also used as input to a health risk communication protocol known as Air Quality Health Index (AQHI). CO2 was also measured simultaneously. The study aimed to assess the relationship between indoor pollutant concentrations and AQHI levels with those outdoors and to evaluate the efficacy of building operating practices in protecting students from pollution exposure. The results indicate that the regular air quality monitoring stations and outdoor pollutant levels at schools exhibit similar patterns. School AQHI levels indoors were generally lower than those outdoors, with PM10 levels showing a larger proportional contribution to the calculated values indoors. NO2 levels in one school were in excess of outdoor values. CO2 monitored in classrooms commonly exceeded indoor guidelines, suggesting poor ventilation. One school that employed air filtration had lower indoor PM concentrations compared to other schools; however, they were still similar to those outdoors. O3 levels indoors were consistently lower than those outdoors. This study underscores the utility of on-site, sensor-based monitoring for assessing the health impacts of indoor and community exposure to urban air pollutants. The findings suggest a need for improved ventilation and more strategic air intake placement to enhance indoor air quality. [ABSTRACT FROM AUTHOR]