93 results on '"Ning Zhai"'
Search Results
2. Corrigendum: Cardiovascular magnetic resonance findings in Danon disease: a case series of a family
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Xiaolong Liu, Ning Zhai, Xiaoqiang Wang, Jiehuan Wang, Mengchun Jiang, Zhanguo Sun, Yueqin Chen, Jingjing Xu, Yinghua Cui, and Lu Li
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Danon disease ,glycogen storage disease ,LAMP2 ,cardiovascular magnetic resonance ,late gadolinium enhancement ,feature tracking ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Published
- 2024
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3. Direct-current electric field stimulation promotes proliferation and maintains stemness of mesenchymal stem cells
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Mengchang Liu, Defu Xie, Huizhen Zeng, Ning Zhai, Lan Liu, and Hong Yan
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adipose-derived mesenchymal stem cells (Ad-MSCs) ,bioreactor ,cell proliferation ,electric field stimulation ,regenerative medicine ,Biology (General) ,QH301-705.5 - Abstract
Mesenchymal stem cells are frequently utilized in the study of regenerative medicine. Electric fields (EFs) influence many biological processes, such as cell proliferation, migration and differentiation. In the present study, a novel device capable of delivering a direct current of EF stimulation to cells cultured in vitro is described. This bioreactor was customized to simultaneously apply a direct-current EF to six individual cell culture wells, which reduces the amount of experimental time and minimizes cost. In testing the device, adipose-derived mesenchymal stem cells stimulated with an EF in the bioreactor exhibited a greater cell proliferation rate while retaining stemness. The results provide a unique perspective on adipose-derived mesenchymal stem cell proliferation, which is needed for tissue engineering and regenerative medicine.
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- 2023
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4. WOX11: the founder of plant organ regeneration
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Qihui Wan, Ning Zhai, Dixiang Xie, Wu Liu, and Lin Xu
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Plant regeneration ,De novo root regeneration ,Callus ,Adventitious lateral root ,Adventitious root ,WOX11 ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Abstract De novo organ regeneration is the process in which adventitious roots or shoots regenerate from detached or wounded organs. De novo organ regeneration can occur either in natural conditions, e.g. adventitious root regeneration from the wounded sites of detached leaves or stems, or in in-vitro tissue culture, e.g. organ regeneration from callus. In this review, we summarize recent advances in research on the molecular mechanism of de novo organ regeneration, focusing on the role of the WUSCHEL-RELATED HOMEOBOX11 (WOX11) gene in the model plant Arabidopsis thaliana. WOX11 is a direct target of the auxin signaling pathway, and it is expressed in, and regulates the establishment of, the founder cell during de novo root regeneration and callus formation. WOX11 activates the expression of its target genes to initiate root and callus primordia. Therefore, WOX11 links upstream auxin signaling to downstream cell fate transition during regeneration. We also discuss the role of WOX11 in diverse species and its evolution in plants.
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- 2023
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5. Natural product preferentially targets redox and metabolic adaptations and aberrantly active STAT3 to inhibit breast tumor growth in vivo
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Yinsong Zhu, Peibin Yue, Cody F. Dickinson, Justin K. Yang, Kyrstin Datanagan, Ning Zhai, Yi Zhang, Gabriella Miklossy, Francisco Lopez-Tapia, Marcus A. Tius, and James Turkson
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Cytology ,QH573-671 - Abstract
Abstract Dysregulated gene expression programs and redox and metabolic adaptations allow cancer cells to survive under high oxidative burden. These mechanisms also represent therapeutic vulnerabilities. Using triple-negative breast cancer (TNBC) as a model, we show that compared to normal human breast epithelial cells, the TNBC cells, MDA-MB-231 and MDA-MB-468 that harbor constitutively active STAT3 also express higher glucose-6-phosphate dehydrogenase (G6PD), thioredoxin reductase (TrxR)1, NADPH, and GSH levels for survival. Present studies discover that the natural product, R001, targets these adaptation mechanisms. Treatment of TNBC cells with R001 inhibited constitutively active STAT3, STAT3-regulated gene expression, and the functions of G6PD and TrxR1. Consequently, in the TNBC, but not normal cells, R001 suppressed GSH levels, but raised NADPH levels, reflective of a loss of mitochondrial respiration and which led to reactive oxygen species (ROS) induction, all of which led to loss of viable cells and inhibition of anchorage-dependent and independent growth. R001 treatment further led to early pyroptosis and late DNA damage, cell cycle arrest, and apoptosis only in the TNBC cells. Oral administration of 5 mg/kg R001 inhibited MDA-MB-468 xenografts growth in mice, with reduced pY705-STAT3, G6PD, TrxR1, and GSH levels. R001 serves as a therapeutic entity that targets the vulnerabilities of TNBC cells to inhibit tumor growth in vivo.
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- 2022
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6. Cardiovascular magnetic resonance findings in Danon disease: a case series of a family
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Xiaolong Liu, Ning Zhai, Xiaoqiang Wang, Jiehuan Wang, Mengchun Jiang, Zhanguo Sun, Yueqin Chen, Jingjing Xu, Yinghua Cui, and Lu Li
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Danon disease ,glycogen storage disease ,LAMP2 ,cardiovascular magnetic resonance ,late gadolinium enhancement ,feature tracking ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
BackgroundCardiac involvement constitutes the primary cause of mortality in patients with Danon disease (DD). This study aimed to explore the cardiac magnetic resonance (CMR) features and progressions of DD cardiomyopathies in a family with long-term follow-up.MethodsSeven patients (five females and two males), belonging to the same family and afflicted with DD, were enrolled in this study between 2017 and 2022. The cardiac structure, function, strain, tissue characteristics on CMR and their evolutions during follow-up were analyzed.ResultsThree young female patients (3/7, 42.86%) exhibited normal cardiac morphology. Four patients (4/7, 57.14%) displayed left ventricle hypertrophy (LVH), and mostly with septal thickening (3/4, 75%). A single male case (1/7, 14.3%) showed decreased LV ejection fraction (LVEF). Nonetheless, the global LV strain of the four adult patients decreased in different degree. The global strain of adolescent male patients was decreased compared to the age-appropriate female patients. Five patients (5/7, 71.43%) exhibited late gadolinium enhancement (LGE), with proportion ranging from 31.6% to 59.7% (median value 42.7%). The most common LGE location was the LV free wall (5/5, 100%), followed by right ventricle insertion points (4/5, 80%) and intraventricular septum (2/5, 40%). Segmental radial strain (rs = −0.586), circumferential strain (r = 0.589), and longitudinal strain (r = 0.514) were all moderately correlated with the LGE proportions of corresponding segments (P
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- 2023
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7. Progression of Danon disease with medical imaging: two case reports
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Shuai Wang, Qinglei Wang, Ning Zhai, Xin Wang, Zhihua Li, Lijun Gan, and Yinghua Cui
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Medicine (General) ,R5-920 - Abstract
Danon disease is a rare X-linked dominant genetic disorder caused by loss-of-function mutations in the lysosome-associated membrane protein 2 gene. Progression of Danon disease is unknown because of its rare incidence in a diverse ethnic population. We report longitudinal data from two patients who were diagnosed with Danon disease by a genetic test. The evaluation protocol included electrocardiographic monitoring, echocardiography, and magnetic resonance imaging. Progression of hypertrophic cardiomyopathy to dilated cardiomyopathy was observed in the first patient. He died from sudden cardiac arrest. The second patient is currently suffering from hypertrophic cardiomyopathy. Development of the hypertrophic phase progressing into the dilated phase in Danon disease may provide useful information for early identification and clinical decisions in patients with this disease.
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- 2021
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8. Repetitive Transcranial Magnetic Stimulation Improves Amygdale Functional Connectivity in Major Depressive Disorder
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Fu-jian Chen, Chuan-zheng Gu, Ning Zhai, Hui-feng Duan, Ai-ling Zhai, and Xiao Zhang
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major depressive disorder ,affective network ,repetitive transcranial magnetic stimulation ,fMRI ,amygdala ,Psychiatry ,RC435-571 - Abstract
Emotional abnormality in major depressive disorder (MDD) is generally regarded to be associated with functional dysregulation in the affective network (AN). The present study examined the changes in characteristics of AN connectivity of MDD patients before and after repetitive transcranial magnetic stimulation (rTMS) treatment over the left dorsolateral prefrontal cortex, and to further assess how these connectivity changes are linked to clinical characteristics of patients. Functional connectivity (FC) in the AN defined by placing seeds in the bilateral amygdale was calculated in 20 patients with MDD before and after rTMS, and in 20 healthy controls (CN). Furthermore, a linear regression model was used to obtain correlations between FC changes and Hamilton depression scale (HAMD) changes in MDD before and after rTMS. Before rTMS, compared with CN, MDD exhibited significantly lower FC between left insula (INS.L), right superior and inferior frontal gyrus (SFG.R and IFG.R), right inferior parietal lobule (IPL.R), and amygdala, and showed an increment of FC between the bilateral precuneus and amygdala in AN. After rTMS, MDD exhibited a significant increase in FC in the INS.L, IFG.R, SFG.R, IPL.R, and a significant reduction in FC in the precuneus. Interestingly, change in FC between INS.L and left amygdala was positively correlated with change in HAMD scores before and after rTMS treatment. rTMS can enhance affective network connectivity in MDD patients, which is linked to emotional improvement. This study further suggests that the insula may be a potential target region of clinical efficacy for MDD to design rationale strategies for therapeutic trials.
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- 2020
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9. Nonspecific Effect of Stress on Brain Gray Matter Volume in Drug-naive Female Patients with First Depressive Episode
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Chuan-Jun Zhuo, Hai-Man Bian, Yan-Jie Gao, Xiao-Lei Ma, Sheng-Zhang Ji, Meng-Yuan Yao, Ning Zhai, Xin-Hai Sun, Xiao-Yan Ma, Hong-Jun Tian, and Gong-Ying Li
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First Episode ,Gray Matter Volume ,Magnetic Resonance Imaging ,Major Depression Disorder ,Stressful Life Events ,Medicine - Abstract
Background: This study aimed to observe the differences in brain gray matter volume in drug-naive female patients after the first episode of major depression with and without stressful life events (SLEs) before the onset of depression. Methods: Forty-three drug-naive female patients voluntarily participated in the present study after the first major depressive episode. The life event scale was used to evaluate the severity of the impact of SLEs during 6 months before the onset of the major depressive episode. High-field magnetic resonance imaging (MRI) scans were obtained, and the VBM and SPM8 software process were used to process and analyze the MRI. Results: Compared to that in patients without SLEs, the volume of brain gray matter was lower in the bilateral temporal lobe, right occipital lobe, and right limbic lobe in the SLE group. However, the gray matter volume did not differ significantly between the two groups after the application of false discovery rate (FDR) correction. Conclusions: Although the results of the present study suggest the absence of significant differences in brain gray matter volume between female drug-naive patients after the first episode of major depression with and without SLEs after FDR correction, the study provides useful information for exploring the definitive role of stress in the onset of depression.
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- 2016
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10. The Anaerobic Product Ethanol Promotes Autophagy-Dependent Submergence Tolerance in Arabidopsis
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Li-Bing Yuan, Liang Chen, Ning Zhai, Ying Zhou, Shan-Shan Zhao, Li-Li Shi, Shi Xiao, Lu-Jun Yu, and Li-Juan Xie
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ADH1 ,autophagy ,ethanol ,hypoxia ,submergence ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
In response to hypoxia under submergence, plants switch from aerobic respiration to anaerobic fermentation, which leads to the accumulation of the end product, ethanol. We previously reported that Arabidopsis thaliana autophagy-deficient mutants show increased sensitivity to ethanol treatment, indicating that ethanol is likely involved in regulating the autophagy-mediated hypoxia response. Here, using a transcriptomic analysis, we identified 3909 genes in Arabidopsis seedlings that were differentially expressed in response to ethanol treatment, including 2487 upregulated and 1422 downregulated genes. Ethanol treatment significantly upregulated genes involved in autophagy and the detoxification of reactive oxygen species. Using transgenic lines expressing AUTOPHAGY-RELATED PROTEIN 8e fused to green fluorescent protein (GFP-ATG8e), we confirmed that exogenous ethanol treatment promotes autophagosome formation in vivo. Phenotypic analysis showed that deletions in the alcohol dehydrogenase gene in adh1 mutants result in attenuated submergence tolerance, decreased accumulation of ATG proteins, and diminished submergence-induced autophagosome formation. Compared to the submergence-tolerant Arabidopsis accession Columbia (Col-0), the submergence-intolerant accession Landsberg erecta (Ler) displayed hypersensitivity to ethanol treatment; we linked these phenotypes to differences in the functions of ADH1 and the autophagy machinery between these accessions. Thus, ethanol promotes autophagy-mediated submergence tolerance in Arabidopsis.
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- 2020
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11. Simple Culture Methods and Treatment to Study Hormonal Regulation of Ovule Development
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Bu-Fan Li, Shi-Xia Yu, Li-Qin Hu, Yan-Jie Zhang, Ning Zhai, Lin Xu, and Wen-Hui Lin
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ovule development ,phytohormones ,treatment in living plant ,culture medium for excised flower/pistil ,brassinosteroids ,Plant culture ,SB1-1110 - Abstract
Ovule development is one of the most important processes in the reproductive development of higher plants and is a determinant of seed quality and quantity. Phytohormones play key roles in this process since loss-of-function mutants in hormone signaling show defective ovule phenotypes and reduced fertility. However, it is difficult to distinguish the direct effects of hormones on ovule development because it is parts of reproductive development and the defective phenotypes would be the indirect effects following the defective vegetative development. The treatment of hormones is a direct method to investigate the hormonal regulation of ovule development, but ovule is embedded inside several layers of floral organs, and traditional methods for hormone (or inhibitor) treatments have various limitations. We have developed simple methods to apply treatments to the flowers in a living plant, where an inflorescence apex is immersed into a solution in an inverted tube. We have also developed a specific system to culture and treat excised flowers/pistils. These procedures will be useful for research on the hormonal regulation of ovule development. We provide examples of how treatments with brassinosteroids (BR) and BR biosynthesis inhibitor. We cultured and treated plant materials using our newly developed methods, and observed the morphology of wild type ovules and fluorescence signals in a marker line to monitor the progress of ovule development. The results demonstrate BR promotes ovule development and our new methods are efficient and repeatable.
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- 2018
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12. Non-Foster Impedance Wideband Matching Technique for Electrically Small Active Antenna
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Wenxing Li, Ning Zhai, Ruilong Chen, and Wenhua Yu
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Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Cellular telephone services industry. Wireless telephone industry ,HE9713-9715 - Abstract
This paper investigates a non-Foster wideband circuit matching technique for an electrically small antenna (ESA). By introducing a negative impedance convertor into an active network, the active network can obtain an equivalent input gain at input port to improve gain, sensitivity and output ratio of signal to noise. In addition, it also increases the effective height of active antenna. The experimental results have verified the proposed method by using a 100 KHz–30 MHz wideband active receiving monopole antenna.
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- 2013
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13. Distribution Information Sharing of Agricultural Products Supply-Chain in Big Data Environment.
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Xue Bai and Ning Zhai
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- 2020
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14. Cardiovascular magnetic resonance findings in Danon disease: a case series of a family.
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Xiaolong Liu, Ning Zhai, Xiaoqiang Wang, Jiehuan Wang, Mengchun Jiang, Zhanguo Sun, Yueqin Chen, Jingjing Xu, Yinghua Cui, and Lu Li
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- 2024
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15. Effects and underlying mechanisms of L-arginine on spontaneous muscle contraction of rat colon
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Xiaojing Quan, Wei Chen, Shuna Gao, Ning Zhai, Jinhai Wang, Hesheng Luo, and Fei Dai
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Organic Chemistry ,Clinical Biochemistry ,Biochemistry - Published
- 2023
16. Developmental trajectory of pluripotent stem cell establishment in Arabidopsis callus guided by a quiescent center-related gene network
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Ning Zhai, Xuan Pan, Minhuan Zeng, and Lin Xu
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Molecular Biology ,Developmental Biology - Abstract
In plant tissue culture, callus formation is induced by a high auxin concentration. Among the three cell layers (the outer, middle and inner cell layers) of the callus, pluripotency acquisition in the middle cell layer is required for the potential ability of the callus to regenerate organs. Here, we reveal the developmental trajectory of middle cell layer initiation and maintenance in callus tissue originating from Arabidopsis thaliana hypocotyls. The S phase of the cell cycle is essential for the expression of quiescent center-related SCARECROW (SCR), PLETHORA1 (PLT1) and WUSCHEL-RELATED HOMEOBOX5 (WOX5) genes during the division of callus founder cells to initiate the callus primordium. After callus initiation, SHOOT-ROOT (SHR) proteins move from the inner to the middle cell layer and act together with SCR to promote the expression of PLT1 and WOX5. WOX5 represses the expression of VASCULAR-RELATED NAC-DOMAIN (VND) genes, thereby preventing callus tissue from differentiating into xylem cells. PLT1 and PLT2 directly activate JACKDAW (JKD), which is necessary for pluripotency acquisition in the middle cell layer. We hypothesize that the middle cell layer could have pluripotent stem cell activity and its establishment requires the quiescent center-related SCR-SHR-WOX5-PLT1/2-JKD gene network.
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- 2023
17. [Review on the assessment model of nutrient recycling with agricultural residues treatment technologies]
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Zheng, Yang, Hong-Qing, Li, Jia-Ning, Zhai, Li-Hua, Zhang, and Qiong, Nan
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Technology ,Geography ,Reproducibility of Results ,Agriculture ,Recycling ,Nutrients - Abstract
The technology for nutrient resource utilization of agricultural residues is crucial to realizing a circular agricultural economy. The assessment model provides essential support to optimize nutrient recovery and treatment technologies. We specifically summarized research progress in the assessment framework of agricultural residues nutrient recycling technology, assessment models and evaluation indicators, data sources of models and their uncertainty analysis, and the application scale of models. We found that process mathematical models and industrial eco-logy models are the common nutrient flow assessment models. Process mathematical and industrial ecology models differed greatly in terms of the reliability of assessment results and the simulation scale. The former mainly focused at laboratory or pilot scale, with higher accuracy. The latter could achieve multi-scale simulation from microscopic to macroscopic and had higher uncertainty, due to the way its data were obtained. Finally, we provided an outlook on the research on the assessment model of agricultural residues nutrient resource utilisation technology. In order to achieve accurate assessment of waste resource utilisation technology in agricultural production systems at the regional scale, a reliable model framework and database should be established by combining process mathematical models with industrial ecology models. Meanwhile, we should carry out research on model expansion at the geographical scales of factory scale, farm scale, village scale, township scale, and regional scale.农业废弃物的养分循环利用技术模式是实现农业循环经济的重要手段,其评估模型为优化养分循环利用技术提供了重要支撑。本文总结了农业废弃物养分循环技术模式评估框架、评估模型及评价指标、模型的数据源及其不确定性分析,以及模型应用尺度的研究进展。当前,常用于评估养分流动的模型主要是过程数学模型和产业生态学模型。过程数学模型和产业生态学模型在评估结果的可靠性和模拟尺度上存在较大差异,前者主要集中在实验室或中试规模,精度较高;后者可以实现从微观到宏观的多尺度模拟,数据的获取方式导致其具有较高的不确定性。最后,本文对农业废弃物养分循环利用技术评估模型的研究进行展望,提出为了在区域尺度上实现对农业生产系统废弃物资源化利用技术的准确评估,可以将过程数学模型与工业生态学模型相结合,建立可靠的模型框架和数据库,同时,在工厂、农场、村落、乡镇、区域等地理尺度进行模型拓展研究。.
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- 2023
18. Pluripotency acquisition in the middle cell layer of callus is required for organ regeneration
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Lin Xu and Ning Zhai
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Cytokinins ,Callus formation ,Plant tissue culture ,Meristem ,Arabidopsis ,Plant Science ,Tissue Culture Techniques ,chemistry.chemical_compound ,Auxin ,Regeneration ,heterocyclic compounds ,Primordium ,Homeodomain Proteins ,chemistry.chemical_classification ,Indoleacetic Acids ,biology ,Arabidopsis Proteins ,fungi ,food and beverages ,biology.organism_classification ,Cell biology ,chemistry ,Callus ,Cytokinin ,Transcription Factors - Abstract
In plant tissue culture, callus forms from detached explants in response to a high-auxin-to-low-cytokinin ratio on callus-inducing medium. Callus is a group of pluripotent cells because it can regenerate either roots or shoots in response to a low level of auxin on root-inducing medium or a high-cytokinin-to-low-auxin ratio on shoot-inducing medium, respectively1. However, our knowledge of the mechanism of pluripotency acquisition during callus formation is limited. On the basis of analyses at the single-cell level, we show that the tissue structure of Arabidopsis thaliana callus on callus-inducing medium is similar to that of the root primordium or root apical meristem, and the middle cell layer with quiescent centre-like transcriptional identity exhibits the ability to regenerate organs. In the middle cell layer, WUSCHEL-RELATED HOMEOBOX5 (WOX5) directly interacts with PLETHORA1 and 2 to promote TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS1 expression for endogenous auxin production. WOX5 also interacts with the B-type ARABIDOPSIS RESPONSE REGULATOR12 (ARR12) and represses A-type ARRs to break the negative feedback loop in cytokinin signalling. Overall, the promotion of auxin production and the enhancement of cytokinin sensitivity are both required for pluripotency acquisition in the middle cell layer of callus for organ regeneration.
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- 2021
19. AOA Based CKF Adaptive Algorithm for AUV Navigation
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Ning Zhai, Xin Zhang, Xiaokai Mu, Di Zhang, Liqian Zhou, Feiyang Lu, and Bo He
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- 2022
20. Research on UFastSLAM Algorithm Based on Gravity Search Algorithm
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Di Zhang, Xin Zhang, Ning Zhai, Ziqing Chen, and Bo He
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- 2022
21. Abstract 514: Novel potent azetidine-based inhibitors bind irreversibly to Stat3 DNA-binding domain (DBD) and are efficacious against tumor growth in mice
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Yue Chen, Ning Zhai, Yinsong Zhu, Peibin Yue, Nagendra Verma, Christine Brotherton-Pleiss, Wenzhen Fu, Kayo Nakamura, Weiliang Chen, Marcus Tius, Francisco J. Lopez-Tapia, and James Turkson
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Cancer Research ,Oncology - Abstract
Unlike classical Signal transducer and activator of transcription (Stat) signaling, which is transient in non-transformed cells, the aberrant activation of the family member, Stat3, occurs in malignant transformation and is implicated in breast, ovarian and many other human cancers. Thus, Stat3 remains a validated and important target for the discovery of novel anticancer drugs. Despite this, the discovery and development of potent Stat3 small molecule inhibitors has proven to be a significant challenge, and no drug is available yet in the market. We have discovered novel Stat3 irreversible azetidine inhibitors with unprecedent submicromolar potency through systematic medicinal chemistry structure activity relationship (SAR). Mechanism of action of most potent azetidine inhibitors depend on whether they are salicylic acids or not, i.e., salicylic acids preferably bind irreversibly to Stat3 Cys426 site in the DNA-binding domain (DBD), and non-salicylic acids, e.g., current lead H182, bind to Stat3 Cys468 DBD site. As expected, the inhibition of Stat3 DNA-binding activity was time dependent, with IC50 in the range of 0.27-0.87 µM at one hour incubation with active Stat3. On the other hand, azetidine salicylic acids also bind to the SH2 domain, although reversibly and at much weaker affinity, as determined in fluorescent polarization (FP) assay, with IC50 of 10-16 µM at one hour incubation, while non-salicylic acids, e.g., lead H182, present no binding affinity up to 600 µM for the SH2 domain. Despite that H182 does not bind to SH2 domain, it still inhibits its phosphorylation. Though lead compound H182 presents significant mouse in vivo efficacy, mouse in vivo pharmacokinetics shows very low plasma AUC, which correlates with quite high mouse in vitro hepatocyte CLint of 138 µL/min/106 cells; however, human in vitro hepatocyte assay gives much better results and is in the middle stability range (CLint of 14.6 µL/min/106 cells). The in vivo PK in other species, e.g., rat, is being determined. H182 represents a plausible molecule for further development. Citation Format: Yue Chen, Ning Zhai, Yinsong Zhu, Peibin Yue, Nagendra Verma, Christine Brotherton-Pleiss, Wenzhen Fu, Kayo Nakamura, Weiliang Chen, Marcus Tius, Francisco J. Lopez-Tapia, James Turkson. Novel potent azetidine-based inhibitors bind irreversibly to Stat3 DNA-binding domain (DBD) and are efficacious against tumor growth in mice [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 514.
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- 2023
22. CRE/LOX-based analysis of cell lineage during root formation and regeneration in Arabidopsis
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Lin Xu and Ning Zhai
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biology ,Arabidopsis ,Regeneration (biology) ,Arabidopsis thaliana ,Organogenesis ,Primordium ,General Medicine ,Root system ,Meristem ,biology.organism_classification ,Root cap ,Cell biology - Abstract
The root system of Arabidopsis thaliana comprises primary, lateral, and adventitious roots. Different types of roots are formed by diverse inductive cues and developmental programs. Here, we adopted the CRE/LOX system to trace cell lineage during the three types of root formation under the control of the promoter of WUSCHEL-RELATED HOMEOBOX5. The results show that the cells forming adventitious roots during de novo root regeneration from detached leaves and lateral roots from the primary root are descendants of the WOX5-expressing root primordium. During the post-embryonic growth of the primary root, some vascular and root cap cells are descendants of the WOX5-expressing stem cell niche in the root apical meristem. Overall, our data suggest that the CRE/LOX system is a useful tool to trace cell lineage in different types of root organogenesis.
- Published
- 2020
23. [Nitrogen flow and polluted nitrogen footprint accounting of rural systems at the municipal scale: The case of Shenyang]
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Jia-Ning, Zhai, Hong-Qing, Li, Wan-Xia, Ren, Cheng-Rui, Tang, and Bing, Xue
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Rural Population ,China ,Nitrogen ,Animals ,Humans ,Agriculture ,Fertilizers - Abstract
Understanding the process of nitrogen flow, emission, and utilization in rural systems is of great importance to reduce pollutant emissions caused by agricultural activities and to promote the sustainable utilization of nutrient resources. Based on the NUFER (nutrient flow in food chain, environment and resources use) model and nitrogen footprint approach, we proposed a nitrogen accounting framework for municipal-scale rural systems, and conducted a quantitative and time series-based comparative study on nitrogen flow, recycling, and footprint from the perspective of three subsystems, namely planting, animal husbandry, and rural human settlement in Shenyang from 1998 to 2018. The results showed that: 1) nitrogen utilization rates of the planting subsystem, animal husbandry subsystem, and rural human settlement subsystem were 36.1%, 59.7%, and 70.1%, respectively in 2018, with a growth rate of 15.9%, 9.1%, and 0.7% respectively compared to 1998; 2) The total polluted nitrogen footprint in Shenyang grew rapidly from 1998 to 2014, but declined from 2014 to 2018. In 2018, the total footprint of nitrogen pollution was 123.5 Gg, increasing by 21.6% compared with 1998; 3) In 2018, the unused nitrogen in the planting subsystem, animal husbandry subsystem, and rural human settlement subsystem were 129.5, 62.2, and 8.7 Gg, respectively, which were equivalent to 420.4, 202.1, and 8.7 Gg of nutrient resources from nitrogen fertilizer, respectively. In general, the nitrogen use efficiency of rural system increased gradually from the production end to the consumption end, but the temporal and spatial variation of nitrogen in rural systems need further studies.理解农村系统的氮素流动、排放和利用过程对减少农业活动引起的潜在环境污染和促进养分资源的可持续利用具有重要意义。本研究基于集成食物链养分流动模型和氮足迹模型,提出市域尺度农村系统氮核算框架,并从种植、动物养殖和农村人居3个子系统对1998—2018年沈阳市的氮素流、循环利用和污染氮足迹开展量化比较研究。结果表明: 2018年沈阳市种植子系统、动物养殖子系统和农村人居子系统的氮素利用率分别为36.1%、59.7%和70.1%,分别比1998年增长了15.9%、9.1%和0.7%;2018年沈阳市农村系统污染氮足迹总量为123.5 Gg,比1998年增长了21.6%;1998—2014年沈阳市农村系统污染氮足迹增长较快,随后有所下降;2018年种植子系统、动物养殖子系统和人居子系统中未利用氮素分别为129.5、62.2和8.7 Gg,相当于420.4、202.1和8.7 Gg氮肥的养分资源量。总体上,农村系统氮素利用率从生产端到消费端逐渐递增,但对农村系统氮素的时空分异情况还有待进一步的研究。.
- Published
- 2021
24. Long non‑coding RNA XIST inhibits osteoblast differentiation and promotes osteoporosis via Nrf2 hyperactivation by targeting CUL3
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Xiao Chen, Fengyu Ma, Guijun Cao, Ning Zhai, and Feng Gao
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NF-E2-Related Factor 2 ,Skeletal disorder ,Downregulation and upregulation ,Genetics ,medicine ,Animals ,Gene silencing ,Viability assay ,Rats, Wistar ,Osteoblasts ,Chemistry ,long non-coding RNA X inactive-specific transcript ,RNA ,Cell Differentiation ,Osteoblast ,Articles ,differentiation ,General Medicine ,Cullin Proteins ,osteoporosis ,Long non-coding RNA ,Rats ,Cell biology ,Disease Models, Animal ,medicine.anatomical_structure ,nuclear factor erythroid 2-related factor 2 ,Female ,RNA, Long Noncoding ,XIST - Abstract
Osteoporosis (OP) is a common skeletal disorder characterized by a low bone mass and the deterioration of bone structure. Long non‑coding (lnc)RNA X inactive‑specific transcript (XIST) is highly expressed in the serum and monocytes of patients with OP. Thus, the purpose of the present study was to explore the mechanisms underlying the role of XIST in the progression of OP. To establish animal models of OP, female rats underwent a bilateral ovariectomy. The bone mineral density of individual rats was measured using dual‑energy X‑ray absorptiometry. The combination of XIST and cullin‑3 (CUL3) was analyzed using a dual‑luciferase reporter assay. Bone histopathological changes were assessed by hematoxylin and eosin staining. Alkaline phosphatase activity was examined by ALP staining. Finally, a series of functional experiments were performed to examine the effects of XIST on cellular behaviors. In the present study, XIST promoted OP and inhibited bone formation by regulating the expression levels of CUL3 and nuclear factor erythroid 2‑related factor 2 (Nrf2) in the rats with OP. Moreover, XIST directly targeted CUL3 and negatively regulated its expression. Of note, CUL3 downregulation reversed the effects of XIST silencing on cell viability, differentiation and mineralization, as well as the expression of Nrf2 and CUL3 in MC3T3‑E1 cells. Collectively, XIST was demonstrated to inhibit the differentiation of osteoblasts and promote OP by inhibiting the degradation of Nrf2 via targeting CUL3.
- Published
- 2021
25. Data-Driven Catalyst Optimization for Stereodivergent Asymmetric Synthesis of α-Allyl Carboxylic Acids by Iridium/boron Hybrid Catalysis
- Author
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Shigeru Yamaguchi, Motomu Kanai, Harunobu Mitsunuma, Yohei Shimizu, Hiroyasu Nakao, Xiang-Ning Zhai, Hongyu Chen, and Yuya Morita
- Subjects
chemistry ,Enantioselective synthesis ,chemistry.chemical_element ,Iridium ,Selectivity ,Boron ,Combinatorial chemistry ,Stereocenter ,Catalysis - Abstract
Asymmetric catalysis enabling divergent control of multiple stereocenters remains challenging in synthetic organic chemistry. While machine learning-based optimization of molecular catalysis is an emerging approach, data-driven catalyst design to achieve stereodivergent asymmetric synthesis producing multiple reaction outcomes, such as constitutional selectivity, diastereoselectivity, and enantioselectivity, is unprecedented. Here, we report the straightforward identification of asymmetric two-component iridium/boron hybrid catalyst systems for α-C-allylation of carboxylic acids. Structural optimization of the chiral ligands for iridium catalysts was driven by molecular field-based regression analysis with a dataset containing overall 32 molecular structures. The catalyst systems enabled selective access to all the possible isomers of chiral carboxylic acids bearing contiguous stereocenters. This stereodivergent asymmetric catalysis is applicable to late-stage structural modifications of drugs and their derivatives.
- Published
- 2021
26. The mitochondrial genome of a rare Chinese stag beetle Kirchnerius guangxii (Coleoptera: Lucanidae)
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Xia Wan, Yong Ning Zhai, Yong Jing Chen, and Shi Ju Zhou
- Subjects
0106 biological sciences ,0301 basic medicine ,03 medical and health sciences ,Mitochondrial DNA ,030104 developmental biology ,biology ,Evolutionary biology ,Stag beetle ,Genetics ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,Molecular Biology - Abstract
We sequenced the mitochondrial genome of a rare Chinese stag beetle Kirchnerius guangxii using the next-generation sequencing. The mitochondrial genome is 14,562 bp in length, containing 13 protein...
- Published
- 2020
27. Transcriptional landscapes of de novo root regeneration from detached Arabidopsis leaves revealed by time-lapse and single-cell RNA sequencing analyses
- Author
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Wu Liu, Yuyun Zhang, Xing Fang, Sorrel Tran, Ning Zhai, Zhengfei Yang, Fu Guo, Lyuqin Chen, Jie Yu, Madalene S. Ison, Teng Zhang, Lijun Sun, Hongwu Bian, Yijing Zhang, Li Yang, and Lin Xu
- Subjects
Plant Leaves ,Sequence Analysis, RNA ,Arabidopsis ,Cell Biology ,Plant Science ,Ethylenes ,Reactive Oxygen Species ,Plant Roots ,Time-Lapse Imaging ,Molecular Biology ,Biochemistry ,Biotechnology - Abstract
Detached Arabidopsis thaliana leaves can regenerate adventitious roots, providing a platform for studying de novo root regeneration (DNRR). However, the comprehensive transcriptional framework of DNRR remains elusive. Here, we provide a high-resolution landscape of transcriptome reprogramming from wound response to root organogenesis in DNRR and show key factors involved in DNRR. Time-lapse RNA sequencing (RNA-seq) of the entire leaf within 12 h of leaf detachment revealed rapid activation of jasmonate, ethylene, and reactive oxygen species (ROS) pathways in response to wounding. Genetic analyses confirmed that ethylene and ROS may serve as wound signals to promote DNRR. Next, time-lapse RNA-seq within 5 d of leaf detachment revealed the activation of genes involved in organogenesis, wound-induced regeneration, and resource allocation in the wounded region of detached leaves during adventitious rooting. Genetic studies showed that BLADE-ON-PETIOLE1/2, which control aboveground organs, PLETHORA3/5/7, which control root organogenesis, and ETHYLENE RESPONSE FACTOR115, which controls wound-induced regeneration, are involved in DNRR. Furthermore, single-cell RNA-seq data revealed gene expression patterns in the wounded region of detached leaves during adventitious rooting. Overall, our study not only provides transcriptome tools but also reveals key factors involved in DNRR from detached Arabidopsis leaves.
- Published
- 2022
28. Abstract 5482: Natural product inhibitor of Stat3, G6PD, and TrxR1 functions induces an early oxidative stress and pyroptosis, and a late-stage DNA damage and cell cycle arrest to block tumor growth in human breast cancer models
- Author
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Yinsong Zhu, Cody F. Dickinson, Justin Yang, Kyrstin Datanagan, Ning Zhai, Peibin Yue, Francisco Lopez-Tapia, Marcus A. Tius, and James Turkson
- Subjects
Cancer Research ,Oncology - Abstract
Bioactive natural products have led to new drugs or been the inspiration for developing new treatments. Signal transducer and activator of transcription (STAT)3 is a an oncogene that has been implicated in many human cancers. We report that the hirsutinolide natural product, R001, blocks STAT3 DNA-binding activty in vitro. Treatment with R001 of the human triple-negative breast cancer (TNBC) cells, MDA-MB-231 and MDA-MB-468 led to the concurrent inhibition of constitutively-active STAT3 and the expression and activities of glucose-6-phosphate 1-dehydrogenase (G6PD) and thioredoxin reductase (TrxR)1, early suppression of glutathione (GSH), and the induction of reactive oxygen species (ROS), which peaked at 6-24 h post R001-treatment. In the short term, R001-treated TNBC cells showed evidence of pyroptotic cell death. Prolonged treatment of TNBC cells with R001 led to DNA damage and G2/M-phase cell cycle arrest, which are late-stage responses that were preceded by the suppression of ATM-Chk2-Cdc25c pathway, and the induction of p21 and γH2AX phosphorylation by the natural product. Accordingly, R001 suppressed the viable cell numbers, colony formation, growth in 3D-matrigel, and the migration of MDA-MB-231 and MDA-MB-468 cells in vitro. The oral administration of R001 at 5 mg/kg signficantly inhibited growth of human MDA-MB-468 xenografts in mice. Results together show that R001 inhibits STAT3, G6PD and TrxR1 functions that lead to early oxidative stress and pyroptosis and late-stage DNA damage and cell cycle arrest, which collectively cause tumor cell death and tumor growth inhibition in human TNBC models. Citation Format: Yinsong Zhu, Cody F. Dickinson, Justin Yang, Kyrstin Datanagan, Ning Zhai, Peibin Yue, Francisco Lopez-Tapia, Marcus A. Tius, James Turkson. Natural product inhibitor of Stat3, G6PD, and TrxR1 functions induces an early oxidative stress and pyroptosis, and a late-stage DNA damage and cell cycle arrest to block tumor growth in human breast cancer models [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 5482.
- Published
- 2022
29. Multiple model AUV navigation methodology with adaptivity and robustness
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Xin Zhang, Bo He, Shuang Gao, Pengcheng Mu, Junchao Xu, and Ning Zhai
- Subjects
Environmental Engineering ,Ocean Engineering - Published
- 2022
30. Progression of Danon disease with medical imaging: two case reports
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Qinglei Wang, Shuai Wang, Lijun Gan, Ning Zhai, Xin Wang, Zhihua Li, and Yinghua Cui
- Subjects
Cardiomyopathy, Dilated ,Male ,medicine.medical_specialty ,Medicine (General) ,Population ,medical imaging ,lysosome-associated membrane protein 2 gene ,Case Report ,Wolff–Parkinson–White syndrome ,Disease ,030204 cardiovascular system & hematology ,Biochemistry ,Electrocardiography ,03 medical and health sciences ,0302 clinical medicine ,R5-920 ,Lysosomal-Associated Membrane Protein 2 ,Internal medicine ,Humans ,Medicine ,Danon disease ,education ,education.field_of_study ,medicine.diagnostic_test ,business.industry ,Biochemistry (medical) ,Genetic disorder ,Hypertrophic cardiomyopathy ,Magnetic resonance imaging ,Dilated cardiomyopathy ,Sudden cardiac arrest ,Cell Biology ,General Medicine ,Cardiomyopathy, Hypertrophic ,hypertrophic cardiomyopathy ,medicine.disease ,Glycogen Storage Disease Type IIb ,dilated cardiomyopathy ,Cardiology ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
Danon disease is a rare X-linked dominant genetic disorder caused by loss-of-function mutations in the lysosome-associated membrane protein 2 gene. Progression of Danon disease is unknown because of its rare incidence in a diverse ethnic population. We report longitudinal data from two patients who were diagnosed with Danon disease by a genetic test. The evaluation protocol included electrocardiographic monitoring, echocardiography, and magnetic resonance imaging. Progression of hypertrophic cardiomyopathy to dilated cardiomyopathy was observed in the first patient. He died from sudden cardiac arrest. The second patient is currently suffering from hypertrophic cardiomyopathy. Development of the hypertrophic phase progressing into the dilated phase in Danon disease may provide useful information for early identification and clinical decisions in patients with this disease.
- Published
- 2021
31. A Rare Chinese Family with Genetic and Clinical Expression of Danon Disease: Case Reports
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Shuchao Pang, Lijun Gan, Ning Zhai, Qinglei Wang, Shuai Wang, Yinghua Cui, and Bo Yan
- Subjects
Proband ,Pathology ,medicine.medical_specialty ,LAMP2 ,medicine.diagnostic_test ,business.industry ,Hypertrophic cardiomyopathy ,General Medicine ,Gene mutation ,medicine.disease ,Gene deletion mutation ,Cardiac magnetic resonance imaging ,medicine ,Danon disease ,cardiovascular diseases ,medicine.symptom ,business ,Myopathy - Abstract
Danon disease is a rare X-linked dominant disorder caused by the lysosome- associated membrane protein 2 (LAMP2) gene. It is characterized by the triad of hypertrophic cardiomyopathy (HCM), myopathy, and intellectual disability. Genetic analysis was performed to confirm LAMP2 gene mutation in a family with Danon disease. We collected the clinical data included electrocardiography (ECG), echocardiography, and cardiac magnetic resonance imaging (cMRI) in a family with Danon disease to reveal the clinical phenotype. Genetic analysis showed that LAMP2 gene deletion mutation (c.257_258delCC) in this family. The proband and his 13-year-old cousin developed HCM with a typical Wolff-Parkinson -White (WPW) pre-excitation pattern. cMRI with late gadolinium enhancement (LGE) further confirms the myocardial fibrosis. The clinical expression of Danon disease is different in different genders. Conclusions: Genetic and pharmaceutical approaches to upregulate LAMP2 gene expression may provide a novel therapy for patients with Danon disease.
- Published
- 2021
32. The Anaerobic Product Ethanol Promotes Autophagy-Dependent Submergence Tolerance in Arabidopsis
- Author
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Ning Zhai, Li-Bing Yuan, Li-Juan Xie, Shan Shan Zhao, Ying Zhou, Lu-Jun Yu, Liang Chen, Shi Xiao, and Li-Li Shi
- Subjects
0106 biological sciences ,0301 basic medicine ,autophagy ,Cellular respiration ,Cell Respiration ,Mutant ,Arabidopsis ,01 natural sciences ,Article ,Catalysis ,Green fluorescent protein ,lcsh:Chemistry ,Inorganic Chemistry ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Immersion ,Humans ,Anaerobiosis ,Physical and Theoretical Chemistry ,lcsh:QH301-705.5 ,Molecular Biology ,Spectroscopy ,Alcohol dehydrogenase ,chemistry.chemical_classification ,Reactive oxygen species ,submergence ,biology ,Arabidopsis Proteins ,Chemistry ,hypoxia ,ADH1 ,Organic Chemistry ,Autophagy ,General Medicine ,biology.organism_classification ,Computer Science Applications ,Cell biology ,Oxygen ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,Seedlings ,biology.protein ,Fermentation ,ethanol ,Reactive Oxygen Species ,010606 plant biology & botany - Abstract
In response to hypoxia under submergence, plants switch from aerobic respiration to anaerobic fermentation, which leads to the accumulation of the end product, ethanol. We previously reported that Arabidopsis thaliana autophagy-deficient mutants show increased sensitivity to ethanol treatment, indicating that ethanol is likely involved in regulating the autophagy-mediated hypoxia response. Here, using a transcriptomic analysis, we identified 3909 genes in Arabidopsis seedlings that were differentially expressed in response to ethanol treatment, including 2487 upregulated and 1422 downregulated genes. Ethanol treatment significantly upregulated genes involved in autophagy and the detoxification of reactive oxygen species. Using transgenic lines expressing AUTOPHAGY-RELATED PROTEIN 8e fused to green fluorescent protein (GFP-ATG8e), we confirmed that exogenous ethanol treatment promotes autophagosome formation in vivo. Phenotypic analysis showed that deletions in the alcohol dehydrogenase gene in adh1 mutants result in attenuated submergence tolerance, decreased accumulation of ATG proteins, and diminished submergence-induced autophagosome formation. Compared to the submergence-tolerant Arabidopsis accession Columbia (Col-0), the submergence-intolerant accession Landsberg erecta (Ler) displayed hypersensitivity to ethanol treatment, we linked these phenotypes to differences in the functions of ADH1 and the autophagy machinery between these accessions. Thus, ethanol promotes autophagy-mediated submergence tolerance in Arabidopsis.
- Published
- 2020
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33. CRE/LOX-based analysis of cell lineage during root formation and regeneration in
- Author
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Ning, Zhai and Lin, Xu
- Subjects
Letter to the Editor - Abstract
The root system of Arabidopsis thaliana comprises primary, lateral, and adventitious roots. Different types of roots are formed by diverse inductive cues and developmental programs. Here, we adopted the CRE/LOX system to trace cell lineage during the three types of root formation under the control of the promoter of WUSCHEL-RELATED HOMEOBOX5. The results show that the cells forming adventitious roots during de novo root regeneration from detached leaves and lateral roots from the primary root are descendants of the WOX5-expressing root primordium. During the post-embryonic growth of the primary root, some vascular and root cap cells are descendants of the WOX5-expressing stem cell niche in the root apical meristem. Overall, our data suggest that the CRE/LOX system is a useful tool to trace cell lineage in different types of root organogenesis.
- Published
- 2020
34. Informatization Innovation for Agricultural Products Marketing Under the Background of 'Internet +'
- Author
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Ning Zhai
- Subjects
business.industry ,media_common.quotation_subject ,Context (language use) ,ComputingMilieux_GENERAL ,Product (business) ,Agricultural marketing ,Agriculture ,The Internet ,Quality (business) ,Business ,Multi-level marketing ,Marketing ,Informatization ,media_common - Abstract
With the introduction of the national “Internet +” strategy, agriculture in the context of “Internet +” has become the key and core for promoting and improving the quality and safety of agriculture. However, at present, the network marketing of agricultural products in China lacks standardized management, the logistics distribution system is not perfect, and the overall rural network coverage is low. The research on agricultural marketing under the background of Internet + is insufficient. The purpose of this paper is to innovate the marketing method of agricultural products for the convenience of the network and to innovate a set of well-managed marketing models. This paper mainly analyzes the four aspects of rural network resources, agricultural products online trading, agricultural product distribution methods, and network construction and proposes innovative methods for the shortcomings. By discussing the development prospects of the network and making recommendations for the construction of agricultural product brands, research shows that making full use of the network marketing methods and increasing the coverage of the network can promote the sales of agricultural products more effectively.
- Published
- 2020
35. Distribution Information Sharing of Agricultural Products Supply-Chain in Big Data Environment
- Author
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Ning Zhai and Xue Bai
- Subjects
ComputingMilieux_GENERAL ,Online and offline ,Product (business) ,Service system ,business.industry ,Information sharing ,Supply chain ,Big data ,Deep integration ,The Internet ,Environmental economics ,business - Abstract
China has vigorously promoted the integration of agricultural products Supply-Chain (SC), developed online and offline agricultural products models by using Internet technology, and effectively improved the management level and circulation efficiency of fresh agricultural products SC in combination with the development. In the report of the 19th National Congress of the Communist Party of China on the implementation of the strategy of rural revitalization, it is proposed to “improve the service system of agricultural socialization and realize the organic connection between small farmers and the development of modern agriculture”. China put forward the action guide for solving this contradiction, taking the deep integration of SC, Internet and Internet of things as the path, network sharing and intelligent cooperation, and promoting the structural reform of the supply side of agricultural and rural areas. The aim of this paper is to explore the research on the distribution information sharing of agricultural products SC, so as to cause different thinking in the integration of big data with agriculture and SC. In this paper, we will use the research method of specific analysis to compare the data and come to a conclusion. The results of this study show that SC is an organizational form which is oriented to meet the personalized needs of customers, aims to improve quality and efficiency, integrates external resources, and realizes efficient collaboration in the whole process of products. In the context of economic, the practical application ability of big data is constantly improved, and higher hopes and objectives for agricultural product SC are put forward.
- Published
- 2020
36. Method to Study Gene Expression Patterns During De Novo Root Regeneration from Arabidopsis Leaf Explants
- Author
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Jie, Yu, Ning, Zhai, Lin, Xu, and Wu, Liu
- Subjects
Organoids ,Plant Leaves ,Luminescent Proteins ,Microscopy, Confocal ,Staining and Labeling ,Gene Expression Regulation, Plant ,Seeds ,Arabidopsis ,Regeneration ,Congo Red ,Cells, Cultured ,Glucuronidase - Abstract
De novo root regeneration (DNRR) is the process in which adventitious roots are regenerated from damaged plant tissues or organs. We have developed a simple DNRR system in which adventitious roots are formed from detached leaf explants of Arabidopsis (Arabidopsis thaliana) on B5 medium without external hormones. In this chapter, we introduce the methods used to observe gene expression patterns during rooting from leaf explants. Usually, β-glucuronidase (GUS) staining is used to visualize gene expression patterns, since fluorescent proteins are difficult to observe because of the high autofluorescence in leaf explants. Here, we describe the use of the ClearSee technique with Congo red staining for deep imaging to observe fluorescent proteins. This method diminishes autofluorescence in leaf explants and preserves the stability of fluorescent proteins, thus allowing us to investigate the endogenous molecular actions guiding DNRR.
- Published
- 2019
37. Method to Study Gene Expression Patterns During De Novo Root Regeneration from Arabidopsis Leaf Explants
- Author
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Lin Xu, Jie Yu, Ning Zhai, and Wu Liu
- Subjects
0106 biological sciences ,0301 basic medicine ,biology ,Regeneration (biology) ,fungi ,food and beverages ,Endogeny ,biology.organism_classification ,01 natural sciences ,Staining ,Cell biology ,03 medical and health sciences ,Autofluorescence ,030104 developmental biology ,Arabidopsis ,Gene expression ,Arabidopsis thaliana ,010606 plant biology & botany ,Explant culture - Abstract
De novo root regeneration (DNRR) is the process in which adventitious roots are regenerated from damaged plant tissues or organs. We have developed a simple DNRR system in which adventitious roots are formed from detached leaf explants of Arabidopsis (Arabidopsis thaliana) on B5 medium without external hormones. In this chapter, we introduce the methods used to observe gene expression patterns during rooting from leaf explants. Usually, β-glucuronidase (GUS) staining is used to visualize gene expression patterns, since fluorescent proteins are difficult to observe because of the high autofluorescence in leaf explants. Here, we describe the use of the ClearSee technique with Congo red staining for deep imaging to observe fluorescent proteins. This method diminishes autofluorescence in leaf explants and preserves the stability of fluorescent proteins, thus allowing us to investigate the endogenous molecular actions guiding DNRR.
- Published
- 2019
38. RNA Sequence Analysis of Cassava Varieties with High-Starch Content Using De Novo Assembly
- Author
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Ya-Jing Guo, Maogui Wei, Xinglu Luo, Zhao-Liang Liu, Yanmei Zhu, Rui-Ning Zhai, and Wu-Jing Fan
- Subjects
0106 biological sciences ,0301 basic medicine ,Sequence database ,Starch ,business.industry ,food and beverages ,RNA ,Sequence assembly ,Plant Science ,Computational biology ,Biology ,01 natural sciences ,Genome ,Biotechnology ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Plant breeding ,KEGG ,business ,Agronomy and Crop Science ,Gene ,010606 plant biology & botany - Abstract
Cassava (Manihot esculenta Crantz) is an important raw material of cassava starch and biological energy worldwide. Among the key traits for crop improvement, elite cassava varieties with high starch content represent a major research target. However, the high genetic heterozygosity of cassava limits its conventional breeding. Identification of novel molecular markers associated with starch biosynthesis facilitates the creation of elite cassava varieties via marker-associated breeding. To increase the number of markers with higher efficiency and stabilization, the transcriptomic profiles of root starch synthesis that were derived from high- and low-starch cassava varieties [Radiation Selection 01 (RS01) and South of China 124 (SC124)] were monitored. More than 292 million reads were deduced by RNA sequencing, which were assembled into 45,007 unigenes. Additionally, 46,925 and 32,421 unigenes from RS01 and SC124, respectively, could be annotated into the Non-redundant protein database, Nucleotide database, Swiss-Prot protein sequence database, Kyoto Encyclopedia of Genes and Genomes, Clusters of Orthologous Groups of proteins database (COG), and Gene Ontology (GO) following their assembly. In the different classification systems of COG and GO, 23,172 and 21,835 unigenes had specific protein functions in RS01 and SC124, respectively. In the statistical classification of function in the differential genes in GO, the involved biological processes included metabolic pathways (ko01100), biosynthesis of secondary metabolites (ko01110), ribosome (ko03010), and RNA transportation (ko03013), among others. Several markers and genes associated with high starch traits were developed. These results provide a feasible and effective framework to improve approaches of cassava starch biosynthesis with the intent of creating high-quality cassava species with novel features.
- Published
- 2017
39. Microarray expression profiling of long non-coding RNAs in epithelial ovarian cancer
- Author
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Su‑Min Wang, Ye Ding, Feng Xu, Xiao‑Yan Gu, Da‑Zheng Yang, Yong‑Ning Zhai, and Kai Xue
- Subjects
0301 basic medicine ,Cancer Research ,Microarray ,Oncogene ,Microarray analysis techniques ,Cancer ,Articles ,Biology ,medicine.disease ,medicine.disease_cause ,Molecular biology ,Gene expression profiling ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Oncology ,030220 oncology & carcinogenesis ,medicine ,Cancer research ,DNA microarray ,Ovarian cancer ,Carcinogenesis - Abstract
Although numerous long non-coding RNAs (lncRNAs) have been identified to be important in human cancer, their potential regulatory roles in epithelial tumorigenesis and tumor progression in ovarian cancer remain unclear. The purpose of the present study was to investigate lncRNAs that were differentially expressed (DE) in epithelial ovarian cancer and to explore their potential functions. The lncRNA profiles in five pairs of human epithelial ovarian cancer tissues and their adjacent normal tissues were described using microarrays. The results of the microarray analysis revealed that 672 upregulated and 549 downregulated (fold-change ≥2.0) lncRNAs were DE between the cancerous and normal tissues. Reverse transcription-quantitative polymerase chain reaction was used to validate the microarray results using four upregulated (RP11-1C1.7, XLOC_003286, growth arrest-specific 5 and ZNF295-AS1) and four downregulated (protein tyrosine kinase 7, maternally expressed gene 3, AC079776.2 and ribosomal protein lateral stalk subunit P0 pseudogene 2) lncRNAs. Furthermore, gene ontology and pathway analyses were used to carry out functional analyses of the candidate genes of DE lncRNAs. The results identified lncRNAs with significantly altered expression profiles in human epithelial ovarian cancer cells compared with those in adjacent normal cells. These data offer new insights into the occurrence and development of epithelial ovarian cancer, and these lncRNAs may provide novel molecular biomarkers for further research on epithelial ovarian cancer.
- Published
- 2017
40. Research on the Status Quo and Countermeasures of Agricultural Products Network Marketing in Jilin Province
- Author
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Ning Zhai
- Subjects
Product (business) ,Status quo ,Agriculture ,business.industry ,media_common.quotation_subject ,Agricultural economy ,Production (economics) ,Research Object ,Multi-level marketing ,business ,Industrial organization ,media_common - Abstract
With the rapid development of e-commerce, the concept of network marketing has been introduced into the sales of agricultural products. It can play an important role in promoting the development of regional agricultural economy by virtue of its ability to break through the limitations of time and space, visually display product information, and facilitate the integration of production, supply and marketing of agricultural products effect. This paper takes Jilin Province agricultural product network marketing as the research object, introduces the status quo of network marketing of agricultural products in Jilin Province, promotes the necessity of agricultural product network marketing development, analyzes the problems existing in agricultural product network marketing in Jilin Province, and proposes a series of solutions Strategy.
- Published
- 2019
41. Nonspecific Effect of Stress on Brain Gray Matter Volume in Drug-naive Female Patients with First Depressive Episode
- Author
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Hong-Jun Tian, Xiaolei Ma, Ning Zhai, Gongying Li, Shengzhang Ji, Xiao-Yan Ma, Xinhai Sun, Yanjie Gao, Mengyuan Yao, Chuanjun Zhuo, and Haiman Bian
- Subjects
Adult ,medicine.medical_specialty ,Gray Matter Volume ,Adolescent ,lcsh:Medicine ,Temporal lobe ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Stress, Physiological ,Internal medicine ,Female patient ,medicine ,Major Depression Disorder ,Humans ,Young adult ,Gray Matter ,Psychiatry ,Major depressive episode ,First episode ,medicine.diagnostic_test ,business.industry ,Depression ,lcsh:R ,Magnetic resonance imaging ,General Medicine ,Middle Aged ,Magnetic Resonance Imaging ,030227 psychiatry ,Drug-naïve ,Stressful Life Events ,First Episode ,Cardiology ,Original Article ,Female ,Brain Gray Matter ,medicine.symptom ,business ,030217 neurology & neurosurgery ,Software ,medicine.drug - Abstract
Background: This study aimed to observe the differences in brain gray matter volume in drug-naive female patients after the first episode of major depression with and without stressful life events (SLEs) before the onset of depression. Methods: Forty-three drug-naive female patients voluntarily participated in the present study after the first major depressive episode. The life event scale was used to evaluate the severity of the impact of SLEs during 6 months before the onset of the major depressive episode. High-field magnetic resonance imaging (MRI) scans were obtained, and the VBM and SPM8 software process were used to process and analyze the MRI. Results: Compared to that in patients without SLEs, the volume of brain gray matter was lower in the bilateral temporal lobe, right occipital lobe, and right limbic lobe in the SLE group. However, the gray matter volume did not differ significantly between the two groups after the application of false discovery rate (FDR) correction. Conclusions: Although the results of the present study suggest the absence of significant differences in brain gray matter volume between female drug-naive patients after the first episode of major depression with and without SLEs after FDR correction, the study provides useful information for exploring the definitive role of stress in the onset of depression.
- Published
- 2016
42. Autophagy contributes to regulation of the hypoxia response during submergence in Arabidopsis thaliana
- Author
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Li Huang, Wen-Sheng Shu, Ying Zhou, Li-Bing Yuan, Ning Zhai, Qin-Fang Chen, Liang Chen, Hua Qi, Wei-Juan Tan, Li-Juan Xie, Bin Liao, Lu-Jun Yu, and Shi Xiao
- Subjects
0106 biological sciences ,0301 basic medicine ,autophagy ,Arabidopsis ,Cellular homeostasis ,Biology ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,Botany ,Molecular Biology ,Alcohol dehydrogenase ,reactive oxygen species ,chemistry.chemical_classification ,submergence ,Reactive oxygen species ,Arabidopsis Proteins ,hypoxia ,Autophagy ,Wild type ,Cell Biology ,Ethylenes ,biology.organism_classification ,Basic Research Paper ,Cell biology ,030104 developmental biology ,chemistry ,biology.protein ,ethanol ,Pyruvate decarboxylase ,Nicotinamide adenine dinucleotide phosphate ,Transcription Factors ,010606 plant biology & botany - Abstract
Autophagy involves massive degradation of intracellular components and functions as a conserved system that helps cells to adapt to adverse conditions. In mammals, hypoxia rapidly stimulates autophagy as a cell survival response. Here, we examine the function of autophagy in the regulation of the plant response to submergence, an abiotic stress that leads to hypoxia and anaerobic respiration in plant cells. In Arabidopsis thaliana, submergence induces the transcription of autophagy-related (ATG) genes and the formation of autophagosomes. Consistent with this, the autophagy-defective (atg) mutants are hypersensitive to submergence stress and treatment with ethanol, the end product of anaerobic respiration. Upon submergence, the atg mutants have increased levels of transcripts of anaerobic respiration genes (alcohol dehydrogenase 1, ADH1 and pyruvate decarboxylase 1, PDC1), but reduced levels of transcripts of other hypoxia- and ethylene-responsive genes. Both submergence and ethanol treatments induce the accumulation of reactive oxygen species (ROS) in the rosettes of atg mutants more than in the wild type. Moreover, the production of ROS by the nicotinamide adenine dinucleotide phosphate (NADPH) oxidases is necessary for plant tolerance to submergence and ethanol, submergence-induced expression of ADH1 and PDC1, and activation of autophagy. The submergence- and ethanol-sensitive phenotypes in the atg mutants depend on a complete salicylic acid (SA) signaling pathway. Together, our findings demonstrate that submergence-induced autophagy functions in the hypoxia response in Arabidopsis by modulating SA-mediated cellular homeostasis.
- Published
- 2015
43. The clinical value of dynamic contrast-enhanced MRI in differential diagnosis of malignant and benign ovarian lesions
- Author
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Hua-qiang Sheng, Bin Zhao, Xi-Bin Hu, Ning Zhai, Jun-Li Hu, Chu-Ran Sun, Xian Li, Xin-hai Sun, and Lai-Min Zhu
- Subjects
Adult ,medicine.medical_specialty ,Pathology ,Adolescent ,Radiography ,Contrast Media ,Diagnosis, Differential ,Image Interpretation, Computer-Assisted ,medicine ,Humans ,Child ,Microvessel ,Aged ,Ovarian Neoplasms ,medicine.diagnostic_test ,Receiver operating characteristic ,business.industry ,Teratoma ,Magnetic resonance imaging ,General Medicine ,Middle Aged ,Magnetic Resonance Imaging ,Intensity (physics) ,Dynamic contrast-enhanced MRI ,Clinical value ,Female ,Radiology ,Differential diagnosis ,business - Abstract
Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) is widely used in preoperative diagnosis of various tumors. We investigated the clinical value of DCE-MRI in differential diagnosis of malignant and benign ovarian lesions. The study involved 48 subjects with surgical pathology-confirmed ovarian tumors with solid components. Early dynamic phase enhancement performances of the ovarian lesions in patients were assessed, including the enhancement pattern, time-signal intensity curve (TIC), signal intensity rate at the initial 60 s (SI60), time to peak within 200 s (TTP200), and slope ratio. There were significant differences in enhancement patterns between benign and malignant ovarian tumors (P
- Published
- 2015
44. The effect of silica-coating on catalyst recyclability in ionic magnetic nanoparticle-supported Grubbs–Hoveyda catalysts for ring-closing metathesis
- Author
-
Sang‐gi Lee, Zhi-Cheng Zhang, Ning Ning Zhai, Chong Min Zhong, and Shu Wei Chen
- Subjects
inorganic chemicals ,Chemistry ,Organic Chemistry ,Inorganic chemistry ,Ionic bonding ,chemistry.chemical_element ,Nanoparticle ,Metathesis ,Biochemistry ,Catalysis ,Ruthenium ,Ring-closing metathesis ,Chemical engineering ,Covalent bond ,Drug Discovery ,Magnetic nanoparticles - Abstract
The Grubbs–Hoveyda type ruthenium–carbene complexes have been covalently immobilized on both silica coated and uncoated magnetic nanoparticles utilizing an imidazolium salt linker. These MNP-supported catalysts showed comparable catalytic activity to the homogeneous imidazolium salt-tagged ruthenium catalyst in the ring-closing metathesis (RCM) of dienes, and could effectively catalyze RCM reactions in the presence of only 0.85 mol % Ru. The MNP-supported ruthenium catalysts could be easily recovered using an external magnet. The recyclability of the silica-coated MNP-supported catalysts was superior to silica uncoated MNP-supported catalysts allowing reuse 14 times, due to the increased compatibility of the silica-coated MNPs with the reaction medium.
- Published
- 2015
45. Innovative Practice in Class Teaching of Higher Vocational and Technical Education in the Era of Internet +
- Author
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Ning Zhai
- Subjects
Medical education ,Engineering ,Class (computer programming) ,business.industry ,Vocational education ,The Internet ,business - Published
- 2017
46. The Research on the Complexity of the Equipment Support Network Based on Complex Networks
- Author
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Jia-Wu, He, primary, Zhi-Ming, Dong, additional, Yong, Zhang, additional, Zhen, Lei, additional, and Xiao-Ning, Zhai, additional
- Published
- 2018
- Full Text
- View/download PDF
47. Potential Mechanism For the Elimination of Suicide Attempts by Modified Electroconvulsive Therapy in Drug-naive Patients with Suicidal Depressive Episode: A Pilot Study Report
- Author
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Hongru Qu, Gongying Li, Xinhai Sun, Chuanjun Zhuo, Ning Zhai, Lina Wang, Jiajia Zhu, Hongjun Tian, and Jie Li
- Subjects
Oncology ,medicine.medical_specialty ,Suicide attempt ,medicine.diagnostic_test ,business.industry ,medicine.medical_treatment ,Thalamus ,Drug-naïve ,Electroconvulsive therapy ,Study report ,Superior frontal gyrus ,Internal medicine ,medicine ,Orbitofrontal cortex ,business ,Functional magnetic resonance imaging ,medicine.drug - Abstract
Background:Modified electroconvulsive therapy (MECT) can rapidly eliminate suicide attempts in patients with suicidal depression. However, no study has investigated the neural mechanisms by which MECT eliminates suicide attempts. Currently, we conducted a study to explore the potential mechanisms by which MECT eliminates suicide attempts in patients with suicidal depressive episodes. Methods:Four drug-naive patients with suicidal depressive episode underwent structural and resting-state functional magnetic resonance imaging scanning before and after MECT treatment. The voxel-based morphometry (VBM) and functional connectivity density mapping(FCDM) methods were used to analyze the imaging data. The gray matter volume (GMV) and resting-state global functional connectivity density (rs-gFCD) were used to evaluate the structural and functional alterations, respectively, that occurred subsequent to MECT. Results:The VBM analysis revealed that MECT induced a GMV increase that was primarily localized to the left dorsolateral prefrontal cortex and bilateral thalamus. The FCDM analysis revealed that MECT induced an rs-gFCD decrease that was mainly localized in the left medial superior frontal gyrus and bilateral orbitofrontal cortex. No overlap existed between brain regions that exhibited structural and functional alterations. Conclusion:The structural and functional alterations subsequent to MECT were mainly located in the brain regions that are related to the suicide attempts in suicidal depressive patients. The brain regions that exhibited structural alterations did not overlap with those that exhibited functional alterations, which suggests that the structural and functional alterations contributed independently to the efficacy of MECT in the elimination of suicide attempts in patients with suicidal depression
- Published
- 2016
48. Study on association between polymorphism of HLA-DRB1 alleles and Behçet's disease
- Author
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Ning Zhai, Shixin Han, Q. S. Ren, Y. B. Shang, J. P. Li, Hong-Duo Chen, and F. J. Song
- Subjects
musculoskeletal diseases ,medicine.medical_specialty ,Erythema ,business.industry ,Dermatology ,Disease ,Behcet's disease ,Human leukocyte antigen ,medicine.disease ,Infectious Diseases ,Polymorphism (computer science) ,Molecular genetics ,Immunology ,medicine ,medicine.symptom ,Allele ,skin and connective tissue diseases ,business ,HLA-DRB1 - Abstract
Background Behcet's disease (BD) is known to be associated with human leucocyte antigen (HLA)-B*51 in many ethnic groups. However, the association of HLA class II gene with BD has been described to be different according to different countries and regions. Objective This study aims to investigate the association between polymorphism of HLA-DRB1 alleles and BD. Methods Forty patients with BD and 100 healthy controls were typed for HLA-DRB1 alleles by the LABTypeTM SSO method. Results The frequency of HLA-DRB1*14 was significantly higher in BD patients than in controls (P
- Published
- 2009
49. Network Intrusion Detection Model based on Combination of Fisher Score and ELM Approach
- Author
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Mei, Hong, primary, Ju, Wang, additional, Wu, Qi Yao, additional, and Ning, Zhai, additional
- Published
- 2016
- Full Text
- View/download PDF
50. A pilot fMRI study of the effect of stressful factors on the onset of depression in female patients
- Author
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Chuanjun Zhuo, Shengzhang Ji, Hongjun Tian, Ning Zhai, Mengyuan Yao, Haiman Bian, Gongying Li, Xinhai Sun, Xiaoyan Ma, and Hongru Qu
- Subjects
Adult ,medicine.medical_specialty ,Stressful life events ,Adolescent ,Cognitive Neuroscience ,Emotions ,Functional magnetic resonance imaging ,Clinical Neurology ,Hippocampus ,Audiology ,Neuropsychological Tests ,Severity of Illness Index ,03 medical and health sciences ,Superior temporal gyrus ,Behavioral Neuroscience ,Cellular and Molecular Neuroscience ,Young Adult ,0302 clinical medicine ,medicine ,Middle frontal gyrus ,Humans ,Radiology, Nuclear Medicine and imaging ,Psychiatry ,Original Research ,Psychiatric Status Rating Scales ,Brain Mapping ,Depressive Disorder, Major ,medicine.diagnostic_test ,Postcentral gyrus ,First major depressive episode ,Neuropsychology ,Precentral gyrus ,Brain ,Middle Aged ,Magnetic Resonance Imaging ,030227 psychiatry ,Emotional lateralization ,Psychiatry and Mental health ,Neurology ,nervous system ,Radiology Nuclear Medicine and imaging ,Acute Disease ,Visual Perception ,Female ,Neurology (clinical) ,Psychology ,030217 neurology & neurosurgery ,Stress, Psychological - Abstract
The goal of this study was to observe the differences in brain activation under negative emotional picture stimuli in drug-naive female patients with a first major depressive episode, comparing patients with and without stressful life experiences prior to the onset of depression. Using a 3.0 T magnetic resonance imaging (MRI) system, 18 patients who experienced stressful life events (SLEs) and 15 patients who did not experience SLEs were scanned under a task-fMRI paradigm designed to distinguish between negative and neutral neural responses to visual stimuli. SPM 8.0 software was used to process the fMRI data; the significantly activated brain regions were recorded and organized in the Montreal Neurological Institute (MNI) standard space. Upon stimulation with negative emotional pictures, depressed patients who had experienced SLEs showed significantly increased activation of the bilateral superior temporal gyrus, left middle temporal gyrus, left middle occipital gyrus, left medial frontal gyrus, right inferior frontal gyrus, bilateral precentral gyrus, bilateral postcentral gyrus, bilateral middle frontal gyrus, right precuneus, left paracentral lobule, bilateral thalamus, bilateral hippocampus, and left cerebellum when compared with depressed patients who did not experience SLEs.The brain regions that showed increased activation in depressed patients who experienced SLEs were primarily located in the neural circuits of the emotion processing system; this result likely indicates that these patients may have an increased negative cognitive bias in the perception, experience, and memory of negative emotional events, as well as their response to those events.
- Published
- 2015
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