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ObjectiveTo investigate the impact of different infusion media on the flow rate of infusion pumps. MethodsTen infusion pumps were randomly selected and tested for infusion rates using deionized water, saline solution, glucose solution, and parenteral nutrition solution. The infusion flow rate was set at 30 mL/h, and the testing methodology conformed to the standards for the calibration and quality control of syringe pumps and infusion pumps. ResultsFor infusion set A, the infusion rate was unaffected by the infusion media, remaining within the acceptable quality control standards. For infusion set B, when infused with deionized water, saline solution, and glucose solution, the infusion errors were within the quality control standards. However, when using parenteral nutrition solution as the infusion medium, the infusion error exceeded the acceptable quality control standards. ConclusionBoth the infusion set and the infusion medium can affect the flow rate of infusion pumps. It is crucial to calibrate the flow rate according to the specific infusion medium to reduce medical risks associated with infusion pumps during clinical use.

The high coverage of mumps-containing vaccine (MuCV) has effectively controlled the mumps incidence, while the prevention and control of breakthrough cases has become a prominent problem. To analyze the epidemiology of mumps breakthrough cases in Chongqing from 2019 to 2023, and provide scientific evidence for the prevention and control of mumps. The data of clinical and laboratory-confirmed mumps cases in Chongqing from 2019 to 2023 were exported from the China Information System for Disease Control and Prevention. The immunization history was screened and matched from the Chongqing Immunization Program Information System. Least-Significant Difference (LSD) and T-test were used for data analysis. There were 12,566 breakthrough cases in Chongqing, accounting for 61.09% of the total mumps cases reported. The most breakthrough infection occurred in the 3–9 years age group. In 2019 and 2020, the mean age of breakthrough cases with two doses was older than that with one dose (p

The present work investigates anisotropic fatigue properties of an additively manufactured (AM) medium-entropy alloy (MEA). The fatigue properties of the materials in 0°, 45° and 90° orientations with respect to the build layers were measured. In order to discover the dominant factors for stress level-dependent fatigue anisotropy, crystal plasticity finite element simulations and fatigue crack growth (FCG) experiments were used to evaluate crack initiation and crack propagation behaviours, respectively. The main conclusions are summarised as follows: First, in comparison to grain anisotropy, the difference in fatigue initiation stage is controlled by defect anisotropy. Second, the direct cause of the difference in the FCG rate is considered to be the differences in deflection angle, affected by the incompatibility of the slip planes between adjacent grains. Third, the AM-MEA displays a stress level-dependent fatigue anisotropy. At high-stress level, the fatigue life of the MEA-45° specimen is significantly higher than that of MEA-0° and MEA-90°, while at low-stress levels, the fatigue resistance of MEA-0° is similar to that of MEA-45°. Stress level-dependent fatigue anisotropy is controlled by different ratios of crack initiation and crack propagation. Our work proposes a novel research strategy that qualitatively evaluates fatigue anisotropy of AM materials.

Abstract Background To assess the immunogenicity of the current primary polio vaccination schedule in China and compare it with alternative schedules using Sabin or Salk-strain IPV (sIPV, wIPV). Methods A cross-sectional investigation was conducted at four sites in Chongqing, China, healthy infants aged 60–89 days were conveniently recruited and divided into four groups according to their received primary polio vaccination schedules (2sIPV + bOPV, 2wIPV + bOPV, 3sIPV, and 3wIPV). The sero-protection and neutralizing antibody titers against poliovirus serotypes (type 1, 2, and 3) were compared after the last dose. Results There were 408 infants completed the protocol. The observed seropositivity was more than 96% against poliovirus types 1, 2, and 3 in all groups. IPV-only groups induced higher antibody titers(GMT) against poliovirus type 2 (Median:192, QR: 96–384, P

Tea tree is a fluorine (F)-enriched plant, leading to much concern about the safety of drinking tea from tea tree (Camellia sinensis (L.) Kuntze). Tea tree is a perennial leaf-harvested crop, and tea production in China is generally categorized as spring tea, summer tea and autumn tea in its annual growth rounds. However, the seasonally dynamic changes of F content and accumulation in the leaves and its drinking safety are poorly understood. In this study, 85 tea varieties cultivated under the same conditions were investigated to analyze the seasonal variation of F content and it’s relationships with F accumulation, aluminum (Al), calcium (Ca) and manganese (Mn) and hazard quotient (HQ) in young leaves (one bud and two leaves, YL) and mature leaves (canopy leaves, ML). The average F contents and accumulations were 350 mg kg−1 and 203 g ha−1 in YL, and they were 2451 mg kg−1 and 2578 g ha−1 in ML, respectively, with F mainly accumulated in ML. As the growing season progresses, the F content showed a gradual increase in YL, while a decrease in ML, inferring that F may be redistributed from mature leaves to young leaves. Additionally, the F content was quite different among tea varieties which are suitable for processing oolong tea, green tea, and black tea, with higher F accumulation in oolong tea varieties than in green and black tea varieties. Moreover, F content and accumulation could be obviously affected by the geographical origin of the tea tree varieties, with significantly higher F content in the varieties from F rich fluorite belts than other regions. Furthermore, F content and accumulation showed a significant positive correlation with the content of Al and Mn (p < 0.05). Based on a daily tea consumption of 8.7 g, the HQ was investigated to show that the proportion of tea leaves with HQ

Equitable access to sanitation for all is a goal of sustainable development, notably in rural areas. However, studies including economic, socio-cultural, and other factors that have been conducted to comprehensively assess rural sanitation at the community level in developing countries are limited. This study aimed to investigate the current state of rural environmental sanitation on the community level and to evaluate the characteristics of the diverse sub-clusters. A multidimensional environmental sanitation survey was conducted on 400 communities in Chongqing, China in 2020, and the priority options for improving sub-clusters’ sanitation were explored using cluster analysis. Among all communities, more than 60 % had positive village appearance, 50.50 % had domestic sewage treatment, and the coverage rate of household sanitary toilets was 72.28 %. The average content for lead, cadmium (Cd), and chromium in soil was 26.01, 0.53, and 54.47 mg/kg, respectively. The communities were clustered into 3 groups (I, II, III) based on similar characteristics including basic information, village appearance, water, sanitation, hygiene management, bio-vector control, and soil pollution. The proportion of cluster I, II, and III was 39.25 % (157/400), 31.75 % (127/400), and 29.00 % (116/400), respectively. Each cluster had its sanitation characteristics, and significant differences among the sub-clusters were observed in Cd of soil (p = 0.001), domestic sewage disposal ratio (p

Abstract Background Previously, many methods have been used to predict the incidence trends of infectious diseases. There are numerous methods for predicting the incidence trends of infectious diseases, and they have exhibited varying degrees of success. However, there are a lack of prediction benchmarks that integrate linear and nonlinear methods and effectively use internet data. The aim of this paper is to develop a prediction model of the incidence rate of infectious diseases that integrates multiple methods and multisource data, realizing ground-breaking research. Results The infectious disease dataset is from an official release and includes four national and three regional datasets. The Baidu index platform provides internet data. We choose a single model (seasonal autoregressive integrated moving average (SARIMA), nonlinear autoregressive neural network (NAR), and long short-term memory (LSTM)) and a deep evolutionary fusion neural network (DEFNN). The DEFNN is built using the idea of neural evolution and fusion, and the DEFNN + is built using multisource data. We compare the model accuracy on reference group data and validate the model generalizability on external data. (1) The loss of SA-LSTM in the reference group dataset is 0.4919, which is significantly better than that of other single models. (2) The loss values of SA-LSTM on the national and regional external datasets are 0.9666, 1.2437, 0.2472, 0.7239, 1.4026, and 0.6868. (3) When multisource indices are added to the national dataset, the loss of the DEFNN + increases to 0.4212, 0.8218, 1.0331, and 0.8575. Conclusions We propose an SA-LSTM optimization model with good accuracy and generalizability based on the concept of multiple methods and multiple data fusion. DEFNN enriches and supplements infectious disease prediction methodologies, can serve as a new benchmark for future infectious disease predictions and provides a reference for the prediction of the incidence rates of various infectious diseases.

Abstract Background The provincial-level sero-survey was launched to learn the updated seroprevalence of hepatitis B virus (HBV) infection in the general population aged 1–69 years in Chongqing and to assess the risk factors for HBV infection to effectively screen persons with chronic hepatitis B (CHB). Methods A total of 1828 individuals aged 1–69 years were investigated, and hepatitis B surface antigen (HBsAg), antibody to HBsAg (HBsAb), and antibody to B core antigen (HBcAb) were detected. Logistic regression and three machine learning (ML) algorithms, including random forest (RF), support vector machine (SVM), and stochastic gradient boosting (SGB), were developed for analysis. Results The HBsAg prevalence of the total population was 3.83%, and among persons aged 1–14 years and 15–69 years, it was 0.24% and 4.89%, respectively. A large figure of 95.18% (770/809) of adults was unaware of their occult HBV infection. Age, region, and immunization history were found to be statistically associated with HBcAb prevalence with a logistic regression model. The prediction accuracies were 0.717, 0.727, and 0.725 for the proposed RF, SVM, and SGB models, respectively. Conclusions The logistic regression integrated with ML models could helpfully screen the risk factors for HBV infection and identify high-risk populations with CHB.

Abstract Toxoplasma gondii is an obligate parasitic protozoon that transmits to animals and humans via ingested food. Cats that act as T. gondii’s final hosts play a critical role in T. gondii transmission by shedding millions of oocysts. Timely diagnosis of infected cats is essential for preventing toxoplasmosis because oocysts are a putative T. gondii source in epidemiology. We developed a new visual LAMP assay targeting the B1 gene to analyze single oocysts in cat feces in this study. The amplification result could be visually estimated based on the color change. LAMP assay analytical sensitivity was 101 copies/µL for the B1 gene plasmid, which was tenfold better than the PCR reaction. There were no cross-reactions with other parasites. The LAMP assay can detect a single T. gondii oocyst in 200 mg of cat feces. The LAMP assay detected a single oocyst in 200 mg cat feces at a higher rate than the PCR assay (83.3% vs. 50.0%).

Abstract Objective Population ageing, as a hot issue in global development, increases the burden of medical resources in society. This study aims to assess the current spatiotemporal evolution and interaction between population ageing and medical resources in mainland China; evaluate the matching level of medical resources to population ageing; and forecast future trends of ageing, medical resources, and the indicator of ageing-resources (IAR). Methods Data on ageing (EPR) and medical resources (NHI, NBHI, and NHTP) were obtained from China Health Statistics Yearbook and China Statistical Yearbook (2011–2020). We employed spatial autocorrelation to examine the spatial–temporal distribution trends and analyzed the spatio-temporal interaction using a Bayesian spatio-temporal effect model. The IAR, an improved evaluation indicator, was used to measure the matching level of medical resources to population ageing with kernel density analysis for visualization. Finally, an ETS-DNN model was used to forecast the trends in population ageing, medical resources, and their matching level over the next decade. Results The study found that China's ageing population and medical resources are growing annually, yet distribution is uneven across districts. There is a spatio-temporal interaction effect between ageing and medical resources, with higher levels of both in Eastern China and lower levels in Western China. The IAR is relatively high in Northwest, North China, and the Yangtze River Delta, but showed a declining trend in North China and the Yangtze River Delta. The hybrid model (ETS-DNN) gained an R2 of 0.9719, and the predicted median IAR for 2030 (0.99) across 31 regions was higher than the median IAR for 2020 (0.93). Conclusion This study analyzes the relationship between population ageing and medical resources, revealing a spatio-temporal interaction between them. The IAR evaluation indicator highlights the need to address ageing population challenges and cultivate a competent health workforce. The ETS-DNN forecasts indicate higher concentrations of both medical resources and ageing populations in eastern China, emphasizing the need for region-specific ageing security systems and health service industries. The findings provide valuable policy insights for addressing a hyper-aged society in the future.

Abstract In Parkinson’s disease (PD), axons of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNc) degenerate before their cell bodies. Calcium influx during pacemaker firing might contribute to neuronal loss, but it is not known if dysfunctions of voltage-gated calcium channels (VGCCs) occur in DA neurons somata and axon terminals. We investigated T-type and L-type VGCCs in SNc-DA neurons of two mouse models of PD: mice with a deletion of the Nurr1 gene in DA neurons from an adult age (cNurr1 mice), and mice bearing the G2019S mutation in the gene coding for LRRK2 (G2019S mice). Adult cNurr1 mice displayed motor and DA deficits, while middle-aged G2019S mice did not. The number and morphology of SNc-DA neurons, most of their intrinsic membrane properties and pacemaker firing were unaltered in cNurr1 and G2019S mice compared to their control and wild-type littermates. L-type VGCCs contributed to the pacemaker firing of SNc-DA neurons in G2019S mice, but not in control, wild-type, and cNurr1 mice. In cNurr1 mice, but not G2019S mice, the contribution of T-type VGCCs to the pacemaker firing of SNc-DA neurons was reduced, and somatic dopamine-D2 autoreceptors desensitized more. Altered contribution of L-type and T-type VGCCs to the pacemaker firing was not observed in the presence of a LRRK2 kinase inhibitor in G2019S mice, and in the presence of a flavonoid with antioxidant activity in G2019S and cNurr1 mice. The role of L-type and T-type VGCCs in controlling dopamine release from axon terminals in the striatum was unaltered in cNurr1 and G2019S mice. Our findings uncover opposite changes, linked to oxidative stress, in the function of two VGCCs in DA neurons somata, but not axon terminals, in two different experimental PD models.

The northern areas of China are traditional endemic regions for brucellosis in both animals and humans, while occasional outbreaks of brucellosis have been observed in neglected southern provinces. On 16 December 2020, Chongqing Center for Disease Control and Prevention (CQCDC) received a report of 15 Brucella seropositive employees in a biological products company. The CQCDC and the local health administrative department launched an investigation that included identification of cases, laboratory testing of samples, and employees’ interview to identify the cause of this incident. A case-control study was implemented to compare high-risk factors between cases and serology-negative personals. Human and animal serum samples and environmental swabs were collected for testing. A total of 61 recessive infectors were found with an infection rate of 43.57% (61/140). Fisher’s exact test showed that there were significant differences in Brucella infection rates among different post classifications (p = 0.02), working places (p = 0.007), and buildings (p

Early embryonic arrest is one of the causes of assist reproduction technology (ART) failure. We have previously reported that the first sperm-derived genetic factor, ACTL7a mutations, could lead to early embryonic arrest. However, whether there are other male genetic factors associated with early embryonic arrest remains elusive. Here, we reported bi-allelic mutations in PLCZ1, a well-known causal gene of total fertilization failure, in four infertile males. Among these mutations, p.403_404del, p.I489S, and p.W536X were newly reported in this study. Histological and Western blotting analysis of the patients’ sperm indicated these variants as loss-of-function mutations. These patients manifested normal conventional semen parameters and ultra-structures in sperm heads. However, among four in vitro fertilization (IVF) cycles, 81.8% (18/22) of the oocytes were polyspermic fertilized, which was rarely reported in PLCZ1-related male patients. In the following six ICSI cycles, artificial oocyte activation (AOA) was applied and successfully rescued the fertilization failure and polyspermy phenotypes, with 31.3% (15/48) of the MII oocytes normally fertilized. However, 60.0% (9/15) of these normally fertilized zygotes were arrested at 2–5-cell stage, with one failing to cleave, indicating that PLCZ1 was not only necessary for fertilization, but also crucial for early embryonic development. However, these rescued zygotes showed a lower potential in developing into blastocysts when cultured in vitro. Thus, fresh cleavage transfer was tried and two live births were successfully achieved thereafter. In conclusion, this study provided novel mutations in PLCZ1 gene to expand the pathogenic mutational spectrum in male infertility and demonstrated that PLCZ1 was a crucial sperm-related genetic factor for early embryonic arrest. We also proposed that cleavage transfer after ICSI and AOA treatment could be a potential treatment method for male patients carrying bi-allelic mutations in PLCZ1.

Summary: The molecular and pathogenic mechanisms of esophageal squamous cell carcinoma (ESCC) development are still unclear, which hinders the development of effective treatments. In this study, we report that DUSP4 is highly expressed in human ESCC and is negatively correlated with patient prognosis. Knockdown of DUSP4 suppresses cell proliferation and patient-derived xenograft (PDX)-derived organoid (PDXO) growth and inhibits cell-derived xenograft (CDX) development. Mechanistically, DUSP4 directly binds to heat shock protein isoform β (HSP90β) and promotes the ATPase activity of HSP90β by dephosphorylating HSP90β on T214 and Y216. These dephosphorylation sites are critical for the stability of JAK1/2-STAT3 signaling and p-STAT3 (Y705) nucleus translocation. In vivo, Dusp4 knockout in mice significantly inhibits 4-nitrochinoline-oxide-induced esophageal tumorigenesis. Moreover, DUSP4 lentivirus or treatment with HSP90β inhibitor (NVP-BEP800) significantly impedes PDX tumor growth and inactivates the JAK1/2-STAT3 signaling pathway. These data provide insight into the role of the DUSP4-HSP90β-JAK1/2-STAT3 axis in ESCC progression and describe a strategy for ESCC treatment.

In this paper, the adaptive synchronization of fractional-order complex-valued neural networks with time-varying delays (FOCVNNTDs) is investigated. First, two novel fractional-order differential inequalities with time delays are established, which can be seen as an extension of Halanay inequality. Besides, complete synchronization and quasi-projective synchronization of FOCVNNTDs are investigated based on the two novel inequalities using a novel adaptive controller. In addition, instead of separating the complex-valued neural networks into two real-valued networks, a non-decomposition method is adopted to study the adaptive synchronization of FOCVNNTDs, which avoids the difficulty and complexity of theoretical analysis. Finally, some numerical simulation examples are provided to demonstrate the validity of our theory.

Abstract Background/objectives Insomnia is highly prevalent in patients with type 2 diabetes mellitus (T2DM). This study therefore evaluated the associations between various micronutrients and insomnia in patients with T2DM. Subjects/methods Between January 2018 and December 2020, a total of 418 T2DM patients with or without insomnia were recruited. Clinical and biochemical parameters, as well as micronutrient levels, were measured in each participant. Insomnia and sleep quality were assessed using the Athens Insomnia Scale and Pittsburgh Sleep Quality Index, respectively. Results Insomnia was found in 24.16% of patients with T2DM. Compared with T2DM patients without insomnia, patients with insomnia had significantly higher levels of vitamin B12 (VitB12). Increased VitB12 was an independent risk factor for insomnia (OR 1.61 [1.06–2.45], P = 0.03). A cut-off value of 517.50 pg/ml VitB12 (P = 0.01, AUC 0.61, standard error 0.04) predicted insomnia risk. Moreover, increased VitB12 levels in patients with insomnia were closely correlated with the use of mecobalamin. Conclusions This study suggests that elevated serum VitB12 level is independently associated with the incidence of insomnia and predicts increased insomnia risk in Chinese patients with T2DM.

Background and purposeSarcopenia is highly prevalent (28.5–40.3%) in patients undergoing hemodialysis and leads to poor clinical outcomes. However, the association between muscle quality and sarcopenia in patients receiving hemodialysis remains unclear. Therefore, we aimed to explore the association between muscle cross-sectional area (CSA) and proton-density fat-fraction (PDFF) in patients with sarcopenia undergoing hemodialysis.MethodsSeventy-six patients undergoing hemodialysis for > 3 months were enrolled. Their handgrip strength (HGS), short physical performance battery (SPPB) performance, and appendicular skeletal muscle mass index (ASMI) were measured. Sarcopenia was defined using the Asian Working Group for Sarcopenia 2019 consensus update. All patients underwent quantitative magnetic resonance imaging. CSA and PDFF were measured for the thigh, trunk, and gluteus muscles.ResultsThe prevalence of probable, confirmed, and severe sarcopenia in this study was 73.7%, 51.3%, and 22.4%, respectively. Older age (OR: 1.061, P < 0.003); lower body mass index (BMI) (OR: 0.837, P = 0.008), albumin (OR: 0.765, P = 0.004), prealbumin (OR: 0.987, P = 0.001), predialysis blood urea nitrogen (BUN) (OR: 0.842, P < 0.001), predialysis creatinine (OR: 0.993, P < 0.001), phosphorus (OR: 0.396, P = 0.047); lower CSA of the thigh (OR: 0.58, P = 0.035), third lumbar (L3) trunk (OR: 0.37, P = 0.004), gluteus minimus and medius (OR: 0.28, P = 0.001), and gluteus maximus (OR: 0.28, P= 0.001); and higher PDFF of the thigh (OR: 1.89, P = 0.036) and L3 trunk (OR: 1.71, P = 0.040) were identified as sarcopenia risk factors. The gluteus minimus and medius CSA was lower in patients with sarcopenia than in those without after adjusting for age and BMI (OR: 0.37, P = 0.017). Higher thigh (P = 0.031) and L3 trunk (P = 0.006) muscle PDFF were significantly associated with lower HGS. Furthermore, higher thigh (P = 0.011) and L3 trunk (P = 0.010) muscle PDFF were also inversely correlated with lower ASMI.ConclusionOur findings demonstrate the high prevalence of sarcopenia and myosteatosis in patients undergoing hemodialysis and might trigger a paradigm shift in intervention strategies for patients receiving hemodialysis.

Vitis adenoclada is a wild grape unique to China. It exhibits well resistance to heat, humidity, fungal disease, drought, and soil infertility. Here, we report the high-quality, chromosome-level genome assembly of GH6 (V. adenoclada). The 498.27 Mb genome contained 221.78 Mb of transposable elements, 28,660 protein-coding genes, and 481.44 Mb of sequences associated with 19 chromosomes. GH6 shares a common ancestor with PN40024 (Vitis vinifera) from approximately 4.26–9.01 million years ago, whose divergence occurred later than Vitis rotundifolia and Vitis riparia. Widely-targeted metabolome and transcriptome analysis revealed that the profiles and metabolism of phenolic compounds in V. adenoclada varieties significantly were differed from other grape varieties. Specifically, V. adenoclada varieties were rich in phenolic acids and flavonols, whereas the flavan-3-ol and anthocyanin content was lower compared with other varieties that have V. vinifera consanguinity in this study. In addition, ferulic acid and stilbenes content were associated with higher expressions of COMT and STSs in V. adenoclada varieties. Furthermore, MYB2, MYB73-1, and MYB73-2 were presumably responsible for the high expression level of COMT in V. adenoclada berries. MYB12 (MYBF1) was positively correlated with PAL, CHS, FLS and UFGT.Meanwhile, MYB4 and MYBC2-L1 may inhibit the synthesis of flavan-3-ols and anthocyanins in two V. adenoclada varieties (YN2 and GH6). The publication of the V. adenoclada grape genome provides a molecular foundation for further revealing its flavor and quality characteristics, is also important for identifying favorable genes of the East Asian species for future breeding.

Abstract Objective Quantitatively staging TAO using MRI remains limited. Our study aims to identify the cut-off signal intensity value for staging TAO using STIR sequence scan. Methods Between June 2018 and July 2020, a number of 51 patients with TAO (102 eyes) and 19 volunteer controls (38 eyes) were recruited. The clinical and biochemical parameters were measured in each patient. Disease activity was diagnosed based on the Clinical Activity Score (CAS). The signal intensities of extraocular muscles were scanned using short-tau inversion recovery (STIR) sequences from MRI. Results Compared to the inactive TAO patients and the controls, the signal intensity ratios (SIRs) of the superior rectus, inferior rectus, medial rectus, lateral rectus on STIR images were significantly increased in the active TAO patients. After adjustment for age and smokers, the SIRs of four extraocular muscles showed strong associations with CAS. By receiver operator characteristic (ROC) curve analysis, all four muscle SIRs demonstrated good efficiency for predicting disease activity [area under curve (AUC) 0.75–0.83, all P 2.9 in the inferior rectus showed optimal diagnostic value for staging the active TAO. Conclusions the signal intensity of extraocular muscles on STIR sequence was a good predictor for TAO activity. A cut-off SIR value of > 2.9 in the inferior rectus could be applied to evaluate the active stage of TAO.

Abstract Ketamine elicits rapid and durable antidepressant actions in treatment-resistant patients with mood disorders such as major depressive disorder and bipolar depression. The mechanisms might involve the induction of metaplasticity in brain regions associated with reward-related behaviors, mood, and hedonic drive, particularly the ventral tegmental area (VTA) and the nucleus accumbens (NAc). We have examined if ketamine alters the insertion of the GluA2 subunit of AMPA receptors (AMPAR), which determines calcium permeability of the channel, at glutamatergic synapses onto dopamine (DA) neurons in the VTA and spiny projection neurons (SPNs) in the Core region of the NAc. Mice received one injection of either saline or a low dose of ketamine 24 h before electrophysiological recordings were performed. We found that GluA2-lacking calcium-permeable (CP) AMPARs were present in DA neurons in the VTA of mice treated with saline, and that ketamine-induced the removal of a fraction of these receptors. In NAc SPNs, ketamine induced the opposite change, i.e., GluA2-lacking CP-AMPARs were inserted at glutamatergic synapses. Ketamine-induced metaplasticity was independent of group I metabotropic glutamate receptors (mGluRs) because an agonist of these receptors had similar effects on glutamatergic transmission in mice treated with saline and in mice treated with ketamine in both VTA DA neurons and in the NAc. Thus, ketamine reduces the insertion of CP-AMPARs in VTA DA neurons and induces their insertion in the NAc. The mechanism by which ketamine elicits antidepressant actions might thus involve an alteration in the contribution of GluA2 to AMPARs thereby modulating synaptic plasticity in the mesolimbic circuit.

With the advancement of the “internet plus” action plan in China, the electronic information system and the mobile phone applications (APPs) are widely used in the management of expanding national program of immunization (EPI). To estimate the use of childhood vaccination APP to improve migrants’ vaccination knowledge, attitude and practice (KAP), a community trial toward migrant children was carried out in Chongqing, China. Migrant children were divided into two groups, one group was provided with health education and vaccination reminders in manual way and the other group was provided with online health education and vaccination reminders by mobile APP. After seven-month interventions, a total of 196 guardians of migrant children aged 2 months to 2 y participated in the questionnaire survey. There were significant differences between two groups in the awareness of vaccine policy, disposal of adverse reaction and attitude toward vaccination. Few significant differences in vaccination coverage of children ≤12 months between two groups, except 2ndbOPV and 2ndMenA. The timely vaccination rate of children >6 months in the APP group ranged from 37.5% to 68.2%, that was from 0% to 30.5% in the non-APP group (P

Aim: The detection and segmentation of cerebral microbleeds (CMBs) images are the focus of clinical diagnosis and treatment. However, segmentation is difficult in clinical practice, and missed diagnosis may occur. Few related studies on the automated segmentation of CMB images have been performed, and we provide the most effective CMB segmentation to date using an automated segmentation system.Materials and Methods: From a research perspective, we focused on the automated segmentation of CMB targets in susceptibility weighted imaging (SWI) for the first time and then constructed a deep learning network focused on the segmentation of micro-objects. We collected and marked clinical datasets and proposed a new medical micro-object cascade network (MMOC-Net). In the first stage, U-Net was utilized to select the region of interest (ROI). In the second stage, we utilized a full-resolution network (FRN) to complete fine segmentation. We also incorporated residual atrous spatial pyramid pooling (R-ASPP) and a new joint loss function.Results: The most suitable segmentation result was achieved with a ROI size of 32 × 32. To verify the validity of each part of the method, ablation studies were performed, which showed that the best segmentation results were obtained when FRN, R-ASPP and the combined loss function were used simultaneously. Under these conditions, the obtained Dice similarity coefficient (DSC) value was 87.93% and the F2-score (F2) value was 90.69%. We also innovatively developed a visual clinical diagnosis system that can provide effective support for clinical diagnosis and treatment decisions.Conclusions: We created the MMOC-Net method to perform the automated segmentation task of CMBs in an SWI and obtained better segmentation performance; hence, this pioneering method has research significance.

Abstract Endothelial cells (EC) coordinate vascular homeostasis and inflammation. In organ transplantation, EC are a direct alloimmune target. We posited that tissue specific heterogeneity of vascular EC may partly underlie the disparate organ-specific alloimmune risk. We examined the vascular endothelial response to inflammation across six primary endothelial beds from four major transplanted organs: the heart, lung, kidney and liver. First, we reanalyzed a public dataset of cardiac allograft rejection and found that endothelial inflammatory response genes were elevated in human cardiac allograft biopsies undergoing rejection compared with stable grafts. Next, the inducible inflammatory phenotypes of EC from heart, lung, kidney, and liver were characterized in vitro, focused on expression of adhesion molecules and chemokines, and recruitment of allogeneic peripheral blood mononuclear immune cells. Large vessel cardiac EC most highly upregulated VCAM-1, particularly compared with hepatic EC, supported greater leukocyte adhesion and had distinct chemokine profiles after stimulation with cytokines and complement. Differentially expressed gene candidates that are known regulators of cytokine signaling and inflammatory programming were verified in publicly available datasets of organ-specific endothelial transcriptomes. In summary, differential baseline expression of immune regulating genes may contribute to differential vascular inflammatory responses depending on organ.

Background: Ambient air pollution has adverse effects on the reproductive system. However, inconsistent conclusions were reached from different studies with regard to air pollutants and pregnancy outcomes, especially the livebirth rate in assisted reproductive technology (ART) in different windows of exposure. Methods: A retrospective cohort study was conducted on 12,665 women who underwent first fresh or frozen embryo transfer cycle in the Yangtze River Delta of China. Daily average levels of six air pollutants in four different periods were obtained: Period 1 and 2: 90 days or one year prior to oocyte retrieval; Period 3 and 4: the day of oocyte retrieval or one year prior to oocyte retrieval to the day of serum hCG test or to the end of the pregnancy. A multiple logistic regression model was used to investigate the association between air pollutant exposure and pregnancy outcomes. Stratified analyses were conducted to explore potential modifier effects. Results: The one year exposure window (Period 2) before oocyte retrieval had a more evident negative association with pregnancy outcomes. Each IQR increase in ambient PM10 (OR: 0.89, 95% CI: 0.84–0.93), PM2.5 (OR: 0.82, 95% CI: 0.77–0.87), SO2 (OR: 0.87, 95% CI: 0.83–0.91) and CO (OR: 0.91, 95% CI: 0.87–0.96) was associated with a respective 11%, 18%, 13% and 9% decrease in the likelihood of live birth. In entire exposure window of Period 4, all air pollutants except for O3 were associated with a decreased likelihood of live birth. Stratified analyses showed that women undergoing frozen embryo transfer cycles, especially those with two embryos transferred, were more vulnerable to air pollutant exposure. Conclusion: This study indicates a negative association between air pollutant exposure before oocyte retrieval and livebirth rate in ART. The adverse impact was more evident in one year exposure compared to three-month refresh cycle of the gametes. Additional protection from air pollution should be undertaken at least one year before ART, particularly for those with frozen embryo transfer cycles.

Extreme precipitation events vary with different sub-regions, sites and years and show complex characteristics. In this study, the temporal variations, trends with significance and change points in the annual time series of 10 extreme precipitation indices (EPIs) at 552 sites and in seven sub-regions were analyzed using the modified Mann–Kendall test and sequential Mann–Kendall analysis. Three representative (extremely wet, normal and extremely dry) years from 1961 to 2017 were selected by the largest, 50%, and smallest empirical frequency values in China. The spatiotemporal changes in the EPIs during the three representative years were analyzed in detail. The results showed that during 1961–2017, both the consecutive wet or dry days decreased significantly, while the number of heavy precipitation days had no significant trend, and the other seven wet EPIs increased insignificantly. The abrupt change years of the 10 EPIs occurred 32 and 40 times from 1963 to 1978 and from 1990 to 2016, respectively, regardless of sub-region. The extremely dry (or wet) events mainly occurred in western (or southwestern) China, implying a higher extreme event risk. The extremely wet, normal and extremely dry events from 1961 to 2017 occurred in 2016, 1997 and 2011 with empirical frequencies of 1.7%, 50% and 98.3%, respectively. In addition, 1998 was the second-most extremely wet year (empirical frequency was 3.7%). The monthly precipitation values were larger from February to August in 1998, forming a much earlier flood peak than that of 2016. The 10 EPIs had close connections with Normalized Difference Vegetation Indexes during the 12 months of 1998 and 2016. This study provides useful references for disaster prevention in China.

Abstract Background This study aimed to explore the biological activities of miR-330-3p in dextan sulphate sodium (DSS)-induced ulcerative colitis and apoptosis and the direct target of miR-330-3p in this process. HT-29 cells and male C57BL/6 mice were used to examine the function of miR-330-3p in vitro and in vivo, respectively. Expression of miRNA and mRNA was measured using quantitative real time PCR (qRT-PCR). Western blotting was used to measure the change of protein expression. Flow cytometry was used to determine cell apoptosis and luciferase assay was used to confirm the direct target of miR-330-3p. Results miR-330-3p expression was increased by DSS in both HT-29 cells and mice. Upregulation miR-330-3p induced cell apoptosis, mice weight loss and ulcerative colitis in vivo, which could prevent by suppression of miR-330-3p. Cell apoptosis related protein expression, cleaved caspase-3 and cleaved PARP was also inhibited by miR-330-3p overexpression and elevated by miR-330-3p inhibition both in vitro and in vivo. Luciferase assay confirmed that 3′ untranslated region (3′-UTR) of XBP1 is the directed target of miR-330-3p and Western blotting results have showed that protein expression of XBP1 was decreased by miR-330-3p mimics and increased by miR-330-3p inhibitor. Conclusion miR-330-3p is upregulated by DSS in both HT-29 cells and mice and promoted ulcerative colitis and cell apoptosis by targeting of 3′-UTR of XBP1, which is a key component of ER stress. Inhibition of miR-330-3p prevent DSS-induced ulcerative colitis and cell apoptosis mediated by upregulation of XBP1 expression.

Objective: The remarkable success of iodine deficiency disorders (IDD) eli mination in China has been achieved through a mandatory universal salt iodi zation (USI) program. The study aims to estimate the relationship between urinary iod ine concentration (UIC) and iodine content in edible salt to assess the current iodine nutritional status of school aged children. Methods: A total of 5565 students from 26 of 39 districts/counties in Chongqing participated in the study, UIC and iodine content in table salt were measured. Thyroid volumes of 3311 students were examined by ultrasound and goiter prevalence was calculated. Results: The overall median UIC of students was 222 μg/L (IQR: 150-313 μg/L). Median UIC was significantly different among groups with non-iodized salt (i odine content 39 mg/kg) salt (P < 0.01). The total goiter rate was 1.9% (60/3111) and 6.0% (186/3111) according to Chinese nationa l and WHO reference values, respectively. Thyroid volume and goiter prevalence were not different within the three iodine nutritional status groups (insufficient, adequate an d excessive, P > 0.05). Conclusions: The efficient implementation of current USI program is able to r educe the goiter prevalence in Chongqing as a low incidence of goiter in school aged children is observed in this study. The widened UIC range of 100–299 μg/L indicating sufficient iodine intake is considered safe with a slim chance of causing goiter or thyroid dysfunction. Further researches were needed to evaluate the applicability of WHO reference in goiter diagnose in Chongqing or identifying more accurate criteria of normal thyroid volume of local students in the future.

Water use efficiency (WUE) is an ecological indicator reflecting the link between carbon and water cycles in terrestrial ecosystems, which is often affected by drought disturbance. However, knowledge about the influences of soil moisture drought on WUE is still lacking. Therefore, this paper aims to quantify the lagged effect and impact of soil moisture drought on terrestrial ecosystem WUE from 1982 to 2018 using ERA5 and Global Land Surface Satellite (GLASS) datasets. Drought conditions are described by the soil moisture anomaly percentage index (SMAPI). The lagged effect of drought on WUE is measured by the month with the maximum significant correlation between SMAPI and WUE. The impact of drought on WUE is estimated through the relative change of WUE during drought and non-drought periods. The results showed that: (1) Drought had an approximately 4-month lagged effect on WUE, which was observed in 70.87% of the global vegetated areas. The lagged effect of drought on WUE was a short period (1–4 months) for shrubland and sparse vegetation, middle and long periods (5–12 months) for forest. (2) When drought occurred, WUE decreased by 36.95% in the Tibetan Plateau and 24.93% in West Africa, while WUE in North Europe, Alaska/N.W. Canada, and West Asia increased by 14.64%, 8.83%, and 8.53%, respectively. The negative and positive impacts of drought on WUE in each vegetation type were commensurate. Our results provide useful information for understanding the response of ecosystem carbon and water cycles to drought..

In recent years, the development of computer technology has promoted the informatization and intelligentization of hospital management systems and thus produced a large amount of medical data. These medical data are valuable resources for research. We can obtain inducers and unknown symptoms that can help discover diseases and make earlier diagnoses. Hypertensive disorder in pregnancy (HDP) is a common obstetric complication in pregnant women, which has severe adverse effects on the life safety of pregnant women and fetuses. However, the early and mid-term symptoms of HDP are not obvious, and there is no effective solution for it except for terminating the pregnancy. Therefore, detecting and preventing HDP is of great importance. This study aims at the preprocessing of pregnancy examination data, which serves as a part of HDP prediction. We found that the problem of missing data has a large impact on HDP prediction. Unlike general data, pregnancy examination data have high dimension and a high missing rate, are in a time series, and often have many non-linear relations. Current methods are not able to process the data effectively. To this end, we propose an improved bi-LSTM-based missing value imputation approach. It combines traditional machine learning and bidirectional LSTM to deal with missing data of pregnancy examination data. Our missing value imputation method obtains a good effect and improves the accuracy of the later prediction of HDP using examination data.

The G2019S mutation in the leucine-rich repeat kinase 2 (LRRK2) gene is associated with late-onset Parkinson’s disease (PD). Although PD affects men and women differently, longitudinal studies examining sex- and age-dependent alterations in mice carrying the G2019S mutation are limited. We examined if behavioral and neurochemical dysfunctions, as well as neurodegeneration, occur in male and female BAC LRRK2-hG2019S (G2019S) mice, compared to their age-matched wild type littermates, at four age ranges. In the open field test, hyperlocomotion was observed in 10–12 month old male and 2–4.5 months old female G2019S mice. In the pole test, motor coordination was impaired in male G2019S mice from 15 months of age and in 20–21 months old female G2019S mice. In the striatum of G2019S male and female mice, the amounts of tyrosine hydroxylase (TH), measured with Western blotting, were unaltered. However, we found a decreased expression of the dopamine transporter in 20–21 month old male G2019S mice. The number of TH-positive neurons in the substantia nigra compacta was unaltered in 20–21 month old male and female G2019S mice. These results identify sex- and age-dependent differences in the occurrence of motor and neurochemical deficits in BAC LRRK2-hG2019S mice, and no degeneration of DA neurons.

Reliable markers to predict or diagnose anastomotic leakage (AL) of stapled circular anastomoses following colorectal resections are an important clinical need. Here, we aim to quantitatively investigate the morphology of anastomotic rings as an early available prognostic marker for AL and compare them to established inflammatory markers. We perform a prospective single-center cohort study, including patients undergoing stapled circular anastomosis between August 2020 and August 2021. The predictive value of the anastomotic ring configuration and the neutrophil-to-lymphocyte ratio (NLR) regarding anastomotic leakage is examined by ROC analyses and compared to the C-reactive protein (CRP) as an established marker. We included 204 patients, of which 19 suffered from anastomotic leakage (LEAK group), while in 185 patients the anastomoses healed well (HEAL group). The minimal height of the anastomotic rings as a binary classifier had a good ROC-AUC of 0.81 but was inferior to the NLR at postoperative day (POD) 5, with an excellent ROC-AUC of 0.93. Still, it was superior to the NLR at POD 3 (0.74) and the CRP at POD 3 (ROC-AUC 0.54) and 5 (ROC-AUC 0.70). The minimal height of the anastomotic rings as indicator for technically insufficient anastomoses is a good predictor of AL, while postoperatively the NLR was superior to the CRP in prediction of AL.

Crop models are important for understanding and regulating agroecosystems. Although the CERES-Wheat model is an important tool for winter wheat research, it has some limitations under water stress conditions. To narrow the gap, this study aimed to improve the performance of the CERES-Wheat model under water stress in arid and semi-arid regions based on the winter wheat experimental data from 2012 to 2014. The Priestley–Taylor (PT) and FAO56 Penman–Monteith (PM) equations were used to calculate the reference crop evapotranspiration and further modified the crop coefficient of the CERES wheat model to improve the simulation accuracy of crop yield and evapotranspiration under water stress conditions. The results showed that: water stress before jointing seriously affected the accuracy of the CERES-Wheat model in simulating biomass and grain yield, so it was necessary to improve the original model. In the original and improved models, the accuracy of the PM equation was lower than that of PT. In addition, the simulation accuracy of the improved model was higher than that of the original model (the average RMAE and RRMSE are less than 30%). In general, among the four scenarios, the PT equation for calculating crop reference evapotranspiration and crop coefficient had the best performance. Water stress occurred at the heading and grain filling stages, and the simulated biomass was in good agreement with the observed results, which better simulated the soil water content under water stress at the later growth stages. Therefore, the change in water stress response function had positive effects on winter wheat growth under simulated water stress conditions. This study provided a reference for applying the CERES-Wheat model in arid and semi-arid areas.

Abstract Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be efficiently corrected by our method. Our study indicates that human cleaving embryos provide an efficient base editing window for robust gene disruption and correction.

Due to the problem of shortening the network lifetime which was caused by the big energy consumption for wireless sensor network (WSN) whose energy and computing power was limit,a lifetime optimization game algorithm combined power control and channel allocation (LOAPC) was proposed.The influence of node power and residual energy on the node interference was explored to construct an interference affection measurement model.Then,expected transmission times was introduced to establish a novel node lifetime model.Finally,LOAPC aimed at reducing interference and prolonging lifetime,and the node power was limited by an optional power set which ensured the network connectivity and economized energy consumption,so as to prolong the network lifetime effectively.At the same time,the simulation results show that the algorithm has the characteristics of low interference,low energy consumption and effectively prolonging the lifetime of the network.

Drought has great negative impacts on crop growth and production. In order to select appropriate drought indices to quantify drought influences on crops to minimize the risk of drought-related crops as much as possible, climate and spring wheat yield-related data from eight sites in the Qinghai Province of China were collected for selecting better drought index between standardized precipitation evapotranspiration index (SPEI, denoting meteorological drought) and soil moisture deficit index (SMDI, denoting agricultural drought) as well as the key parameters (timescale and month) in denoting drought impacts on spring wheat yields. The spring wheat yields during 1961–2018 were simulated by the DSSAT–CERES–Wheat model. Pearson correlations were used to investigate the relationship between SPEI and SMDI and between spring wheat yields and drought indices at different timescales. The results showed that: (1) SMDI reflected more consistent dry/wet conditions than SPEI when the timescales changed and (2) There were one- and two-month lags in SMDI compared to SPEI (with the higher correlation coefficients values of 0.35–0.68) during May to August and (3) May (the jointing period of spring wheat) and the two-month timescale of SMDI0–10 (with the higher correlation coefficients values of 0.21–0.37) were key parameters denoting drought influences on spring wheat yield and (4) The correlations between the linear slopes of spring wheat yield reduction rate and linear slopes of SMDI0–10 in May at the studied eight sites were considerable between 1961–2018 (r = 0.85). This study provides helpful references for mitigating the drought risk of spring wheat.

The dysfunction of the intestinal epithelial barrier contributes to local or systemic infection and inflammation. Some lactic acid bacteria (LAB) strains had been shown to improve the conditions of barrier function and, for this reason, are recognized as probiotics. Weissella cibaria, a species belonging to the LAB group, is known to promote several health benefits. However, the role of W. cibaria in regulating the integrity of the intestinal epithelial barrier has not yet been investigated. In this study, W. cibaria MW01 was isolated from Chinese sauerkraut and was selected based on its functional features, such as gastric juice and bile salt tolerance, besides antagonistic activity against pathogenic bacteria. In a cellular model of the intestinal barrier, it was observed that W. cibaria was able to adhere more efficiently than Lactobacillus rhamnosus GG in Caco-2 cells. Moreover, the LPS-induced inflammation in Caco-2 cells was attenuated by the treatment with W. cibaria MW01, which reduced the synthesis of TNF-α, IL-6, and IL-8. In addition, it was noted that the treatment with W. cibaria MW01 recovered the integrity of the Caco-2 cell monolayer exposed to LPS. Furthermore, W. cibaria MW01 significantly alleviated LPS-induced downregulation of tight junction proteins (TJP) (claudin, occludin, and tight junction protein-1). Mechanistically, W. cibaria MW01 inhibited the translocation of NF-κB to the nucleus and deactivated the MLCK-pMLC pathway during LPS exposure. Thus, W. cibaria MW01, as a potential probiotic, can protect intestinal epithelial barrier function by regulating inflammation and expression of TJP via the NF-κB-mediated MLCK-pMLC pathway.

China has a marked continental monsoon climate characterized by dry and wet hazards that have destructive impacts on grape yields and quality. The purpose of this study was to analyze the spatiotemporal characteristics of dryness/wetness in the wine regions of China and explore the links between these variations and large-scale climatic factors. The crop-specific standardized precipitation evapotranspiration index (SPEI) was used to characterize the dryness/wetness using meteorological data collected at 168 meteorological stations located in or near the wine regions from 1981–2015. Results showed that most wine regions of China experienced a wetting trend. The drought and wet event characteristics were region- and site-specific. The main wine regions of China (e.g., Xinjiang, Helan Mountain and Hexi Corridor) were characterized by relatively high drought severity; the extreme drought frequencies of the three regions were higher as well (11.5%, 3.3%, and 3.6%, respectively). Xinjiang was also characterized by a high wetness severity and an extremely high wetness frequency of 16%, but the wetness severity decreased over time. A 4–6-year periodical oscillation was commonly detected over the wine regions. The dryness/wetness characteristics were highly associated with the Southern Oscillation Index, Niño 3.4 and the Indian Ocean Dipole, with highest correlation coefficients of −0.40, 0.36 and 0.43 at lag times of 11, 8, and 11 months, respectively. The serious dry and wet events that occurred in 2001 and 1998, respectively, were speculated to be associated with anomalous atmospheric circulation patterns. These results can be used to inform grapevine stakeholders at various levels (e.g., farmer and industry) for developing strategies to mitigate and adapt dryness/wetness events in the wine regions of China. It is expected that the approach proposed in this study can also be applicable to wine regions of other countries.

Introduction : Preimplantation genetic diagnosis/screening (PGD/PGS) can effectively detect chromosomal abnormalities in an embryo but only if an embryo is available. However, not all couples can obtain an embryo that is available for testing. The purpose of this study was to identify factors which might affect the formation of PGD/PGS embryos to predict the possibility of obtaining embryos that could be detected. Material and methods : In a retrospective study, we included all couples who underwent PGD/PGS at our center from January 2015 to December 2016. We compared these patients according to the non-blastocyst group and the blastocyst group. Results : There were 302 couples who had blastocysts in their first PGD/PGS cycle. Fifty-seven couples had no blastocysts in their PGD/PGS cycles: 43 couples had no blastocysts in one cycle; 10 in two cycles; 4 in three cycles. The non-blastocyst group was older than the blastocyst group (32.37 vs. 30.69, p = 0.048). Anti-mullerian hormone (ng/ml) in the non-blastocyst group was significantly lower than in the blastocyst group (4.80 ±3.67 vs. 3.07 ±2.30, p = 0.00). Women whose chromosome were aneuploid (47, XXX or 45, X) had a similar AMH level compared with others, but the number of retrieved oocytes was much lower; the normal karyotype was 14.25 and aneuploidy was 5.40 (p = 0.01) in women < 30 years old. There was the same condition in women aged 30–38 years (14.60 vs. 3.44, p < 0.001). Male’s different chromosome karyotype had no influence on double pronuclear number or the rate of blastocyst formation. Presence of endometriosis, polycystic ovary syndrome and tubal factor showed no difference between the blastocyst and non-blastocyst group. Nor did oligospermia and asthenospermia. Conclusions : Elderly women, those with lower AMH and women with 47, XXX or 45, X have fewer ova, leading to the possibility of no blastocyst. These couples should be fully informed and weigh the advantages and disadvantages before undergoing PGD/PGS.

We used the APSIM-Maize model to simulate maize potential yield (Yp) and rain-fed yield (Yw) when adaptation options of sowing date and planting density were adopted under Representative Concentration Pathway (RCP) 4.5 and 8.5 in the Guanzhong Plain of China. The results showed that Yp would decrease by 10.6–14.9% and 15.0–31.4% under RCP4.5 and RCP8.5 for summer maize, and 13.9–19.7% and 18.5–36.3% for spring maize, respectively. The Yw would decrease by 17.1–19.0% and 23.6–41.1% under RCP4.5 and RCP8.5 for summer maize, and 20.9–24.5% and 27.8–45.5% for spring maize, respectively. The loss of Yp and Yw could be reduced by 2.6–9.7% and 0–9.9%, respectively, under future climate for summer maize through countermeasures. For spring maize, the loss of Yp was mitigated by 14.0–25.0% and 2.0–21.8% for Yw. The contribution of changing sowing date and plant density on spring maize yield was more than summer maize, and the optimal adaptation options were more effective for spring maize. Additionally, the influences of changing sowing date and planting density on yields become weak as climate changes become more severe. Therefore, it is important to investigate the potential of other adaptation measures to cope with climate change in the Guanzhong Plain of China.

The regional and national scales variation and propagation characteristics of different types of droughts are critical for improving drought resilience, while information is limited in China. The objective of this research was to investigate the evolution and propagation characteristics of three types of droughts using standardized indices at multi-timescales in different sub-regions of China. The indices included Standardized Precipitation/Soil Moisture/Runoff Index (SPI/SSI/SRI) using the optimal probability density function, representing meteorological, agricultural, and hydrological droughts based on precipitation, soil water storage, and baseflow-groundwater runoff, respectively. Wavelet analysis was used to reveal their periodical characteristics. Modified Mann-Kendall trend test was used to compare the trend among drought indices. Correlation coefficients between SPI and SSI/SRI were calculated to identify the time-lags of SPI with SSI and SRI. In general, droughts indicated by SPI agreed well with the historical drought events at different sub-regions. The main periods of SSI were closer to SPI than SRI, indicating stronger connections of agricultural drought with meteorological drought. A weaker connection between meteorological and agricultural/hydrological droughts at shorter timescales was observed in northwestern arid and semi-arid regions. The propagation from meteorological to agricultural or hydrological droughts were well denoted by the lagged time (months) from SPI to SSI or SRI at a timescale ranged from 0 (mostly located in south China) to 5 months (mostly located in northeastern China) for 1-, 3-, 6-, 12-, or 24-month timescale; this was a new finding for China. The methods of wavelet combining trend test and Pearson coefficient showed meaningful power for revealing the drought propagation characteristics and the obtained results can be a good reference for other regions of the world since this study compared different climate zones from arid to humid conditions. The study provides crucial information and guidance to develop drought management strategies at regional to national scale and their critical time of action.

Soil water repellency (SWR) is a widespread property in soils around the world. It influences soil water movement and causes unstable flow. The mechanics of finger flow occurrence in water-repellent soils are not clearly understood. Slab chamber infiltration experiments consisting of increasing SWR persistence in clay and sandy loam soils were conducted to compare wetting front advancement and mechanics of finger flow development. The temporal variation curves of cumulative infiltration (CI) decreased with an increase in SWR persistence. For wettable soils, the wetting front advanced regularly with time, but for water-repellent soils, it became unstable and finger flow occurred as the SWR persistence increased. Water movement in soils with higher SWR persistence tended to be more unstable. Water repellency contributed to finger flow development, especially for sandy loam. There were strong correlations among CI, finger length, and the cumulative wetting area. In the strongly, severely, and extremely water-repellent sandy loam soils, the power function relationship fit better than the linear function. The average soil water content decreased with a higher SWR persistence, which meant that less water was available in the profile. Finger flow development was related to the more severe water-repellent conditions and tended to be more easily formed in the water-repellent sandy loam soils than the clay loam soils due to the faster infiltration rate.

Background. Cortical hemispherectomy leads to degeneration of ipsilateral subcortical structures, which can be observed long term after the operation. Therefore, reorganization of the brainstem auditory pathway might occur. The aim of this study was to assess reorganization of brainstem auditory pathways by measuring the auditory brainstem response (ABR) in long-term hemispherectomized patients. Methods. We performed bilateral monaural stimulation and measured bilateral ABR in 8 patients ~20 years after hemispherectomy and 10 control subjects. Magnetic resonance imaging (MRI) was performed in patients to assess structural degeneration. Results. All patients showed degenerated ipsilateral brainstem structures by MRI but no significant differences in bilateral recording ABR wave latencies. However, nonsurgical-side stimulation elicited significantly longer wave V latencies compared to surgical-side stimulation. Differences in bilateral ABR were observed between hemispherectomized patients and control subjects. Waves III and V latencies elicited by nonsurgical-side stimulation were significantly longer than those in control subjects; surgical-side stimulation showed no significant differences. Conclusions. (1) Differences in ABR latency elicited by unilateral stimulation are predominantly due to bilateral brainstem auditory pathway activity rather than to changes in brainstem volume; (2) ABR Waves III and V originate predominantly in the contralateral brainstem; and (3) subcortical auditory pathways appear to reorganize after long term hemispherectomy.

A new baculovirus-silkworm multigene expression system named Bombyx mori MultiBac is developed and described here, by which multiple expression cassettes can be introduced into the Bombyx mori nuclear polyhedrosis virus (BmNPV) genome efficiently. The system consists of three donor vectors (pCTdual, pRADM and pUCDMIG) and an invasive diaminopimelate (DAP) auxotrophic recipient E. coli containing BmNPV-Bacmid (BmBacmid) with a homologous recombination region, an attTn7 site and a loxp site. Two genes carried by pCTdual are firstly inserted into BmBacmid by homologous recombination, while the other eight genes in pRADM and pUCDMIG are introduced into BmBacmid through Tn7 transposition and cre-loxp recombination. Then the invasive and DAP auxotrophic E. coli carrying recombinant BmBacmid is directly injected into silkworm for expressing heterologous genes in larvae or pupae. Three structural genes of rotavirus and three fluorescent genes have been simultaneously expressed in silkworm larvae using our new system, resulting in the formation of virus-like particles (VLPs) of rotavirus and the color change of larvae. The VLPs were purified from hemolymph by ultracentrifugation using CsCl gradients, with a yield of 12.7 µg per larva. For the great capacity of foreign genes and the low cost of feeding silkworm, this high efficient BmMultiBac expression system provides a suitable platform to produce VLPs or protein complexes.