Search

Your search keyword '"Nilsson, Jesper R."' showing total 20 results
Start Over Author "Nilsson, Jesper R."
20 results on '"Nilsson, Jesper R."'

5. Multiphoton characterization and live cell imaging using fluorescent adenine analogue 2CNqA.

Author

Nilsson, Jesper R., Benitez-Martin, Carlos, Sansom, Henry G., Pfeiffer, Pauline, Baladi, Tom, Le, Hoang-Ngoan, Dahlén, Anders, Magennis, Steven W., and Wilhelmsson, L. Marcus

Abstract

Fluorescent nucleobase analogues (FBAs) are established tools for studying oligonucleotide structure, dynamics and interactions, and have recently also emerged as an attractive option for labeling RNA-based therapeutics. A recognized drawback of FBAs, however, is that they typically require excitation in the UV region, which for imaging in biological samples may have disadvantages related to phototoxicity, tissue penetration, and out-of-focus photobleaching. Multiphoton excitation has the potential to alleviate these issues and therefore, in this work, we characterize the multiphoton absorption properties and detectability of the highly fluorescent quadracyclic adenine analogue 2CNqA as a ribonucleotide monomer as well as incorporated, at one or two positions, into a 16mer antisense oligonucleotide (ASO). We found that 2CNqA has a two-photon absorption cross section that, among FBAs, is exceptionally high, with values of σ2PA(700 nm) = 5.8 GM, 6.8 GM, and 13 GM for the monomer, single-, and double-labelled oligonucleotide, respectively. Using fluorescence correlation spectroscopy, we show that the 2CNqA has a high 2P brightness as the monomer and when incorporated into the ASO, comparing favorably to other FBAs. We furthermore demonstrate the usefulness of the 2P imaging mode for improving detectability of 2CNqA-labelled ASOs in live cells. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

6. Stealth Fluorescence Labeling for Live Microscopy Imaging of mRNA Delivery

Author

Baladi, Tom, primary, Nilsson, Jesper R., additional, Gallud, Audrey, additional, Celauro, Emanuele, additional, Gasse, Cécile, additional, Levi-Acobas, Fabienne, additional, Sarac, Ivo, additional, Hollenstein, Marcel R., additional, Dahlén, Anders, additional, Esbjörner, Elin K., additional, and Wilhelmsson, L. Marcus, additional

Published
2021
Full Text
View/download PDF

7. Stealth fluorescence labeling for live microscopy imaging of mRNA delivery

Author

Baladi, Tom, primary, Nilsson, Jesper R., additional, Gallud, Audrey, additional, Celauro, Emanuele, additional, Gasse, Cécile, additional, Levi-Acobas, Fabienne, additional, Sarac, Ivo, additional, Hollenstein, Marcel, additional, Dahlén, Anders, additional, Esbjörner, Elin K., additional, and Wilhelmsson, L. Marcus, additional

Published
2020
Full Text
View/download PDF

8. Getting DNA and RNA out of the dark with 2CNqA: a bright adenine analogue and interbase FRET donor

Author

Wypijewska del Nogal, Anna, primary, Füchtbauer, Anders F, additional, Bood, Mattias, additional, Nilsson, Jesper R, additional, Wranne, Moa S, additional, Sarangamath, Sangamesh, additional, Pfeiffer, Pauline, additional, Rajan, Vinoth Sundar, additional, El-Sagheer, Afaf H, additional, Dahlén, Anders, additional, Brown, Tom, additional, Grøtli, Morten, additional, and Wilhelmsson, L Marcus, additional

Published
2020
Full Text
View/download PDF

9. Interbase FRET in RNA: from A to Z

Author

Füchtbauer, Anders F, primary, Wranne, Moa S, additional, Bood, Mattias, additional, Weis, Erik, additional, Pfeiffer, Pauline, additional, Nilsson, Jesper R, additional, Dahlén, Anders, additional, Grøtli, Morten, additional, and Wilhelmsson, L Marcus, additional

Published
2019
Full Text
View/download PDF

15. Light-induced cytotoxicity of a photochromic spiropyran

Author

Nilsson, Jesper R., Li, Shiming, Önfelt, Björn, Andreasson, Joakim, Nilsson, Jesper R., Li, Shiming, Önfelt, Björn, and Andreasson, Joakim

Abstract

In this work we present a novel water soluble spiropyran photoswitch that can be photonically activated inside live cells from a form that has no significant effect on the cellular survival to a form that induces a dramatic toxic response., QC 20111028

Published
2011
Full Text
View/download PDF

20. Characterizationof the Thermal and Photoinduced Reactionsof Photochromic Spiropyrans in Aqueous Solution.

Author

Hammarson, Martin, Nilsson, Jesper R., Li, Shiming, Beke-Somfai, Tamás, and Andréasson, Joakim

Subjects
*PHOTOINDUCED electron transfer, *PHOTOCHROMIC materials, *SPIROPYRANS, *AQUEOUS solutions, *HYDROGEN-ion concentration, *MEROCYANINES, *ISOMERS
Abstract

Six water-soluble spiropyran derivativeshave been characterizedwith respect to the thermal and photoinduced reactions over a broadpH-interval. A comprehensive kinetic model was formulated includingthe spiro- and the merocyanine isomers, the respective protonatedforms, and the hydrolysis products. The experimental studies on thehydrolysis reaction mechanism were supplemented by calculations usingquantum mechanical (QM) models employing density functional theory.The results show that (1) the substitution pattern dramatically influencesthe pKa-values of the protonated formsas well as the rates of the thermal isomerization reactions, (2) wateris the nucleophile in the hydrolysis reaction around neutral pH, (3)the phenolate oxygen of the merocyanine form plays a key role in thehydrolysis reaction. Hence, the nonprotonated merocyanine isomer issusceptible to hydrolysis, whereas the corresponding protonated formis stable toward hydrolytic degradation. [ABSTRACT FROM AUTHOR]

Published
2013
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results