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The ratio of protein to creatinine (PCR) in urine is a measure of kidney health of a human body. We report the development of a point-of-care (POC) kidney function test (KFT) kit, which is composed of a paper-based PCR sensor, an opto-electrochemical measurement unit, a display, and a facility to transfer data to a smartphone. For the total protein sensor, the pieces of filter papers were coated with a mixture of alcoholic bromophenol blue and citric buffer leading to a chrome-yellow coloration. While for the creatinine sensor the papers were coated with alkaline picrate solution leading to a lemon-yellow coloration of the surface. Dispensing protein (creatinine) solution of known concentration on the respective sensors led to the conversion of chrome-yellow to bluish-green coloration (lemon-yellow to orange coloration) for the total protein (creatinine) sensor. The intensity of such colorations varied with the concentration of the analytes in the solutions. The variations in the intensities of colors with analyte concentration were quantified by integrating a light emitting diode (LED) at one side of each sensor and a light intensity sensor (LIS) on the other side. The intensity of the transmitted rays, passing through the sensors, was found to vary the electrical output of the LIS monotonically with the loading of the total protein or creatinine in the analyte. This helped in obtaining the calibration plots for the PCR sensors. Further, the sensors were employed for the on-spot detection of the unknown PCR in the real human urine samples.

A paper based chemiresistor has been fabricated to selectively sense ethanol in human breath. The chemiresistor was composed of a sensing mixture of multiwall-carbon-nano-tubes (MWCNTs), poly (diallyl-dimethyl-ammonium chloride) (PDDA), alcohol dehydrogenase (ADH), and coenzyme (NADH). The aluminum electrode was deposited on the paper surface, followed by drop-casting of the aforementioned sensing mixture. The resistance of the sensors was measured by exposing the same in gas-vapor mixture as well as the sample solution. The surface-modified MWCNTs specifically broke down ethanol present in the gas-vapor mixture or in a solution to generate a quantitative electronic response proportional to the ethanol concentration. Subsequently, the interference of other volatile organic materials was also tested to prove the selectivity and sensitivity of the sensor towards ethanol in the presence of different volatile organic compounds (VOCs). The variation of the resistance during the interaction between sensor and ethanol was also characterized by measuring the surface potential of the channel material under ethanol exposure using Kelvin probe force microscopy (KPFM). The sensor was integrated with a voltage divider circuit, a display, and a microcontroller unit to make a proof-of-concept prototype for the point-of-care (POC) detection of ethanol in human breath.

Contamination of water bodies due to the disposal of natural and anthropogenic pollutants is one of the major global concerns in the recent times. Regular on-spot monitoring of water quality has become mandatory wherein diverse classes of materials such as organic, inorganic, heavy metals, or biological wastes are detected employing standard protocols. However, most of the existing laboratory techniques are either costly and/or time consuming owing to the process involving the expert driven sophisticated analysis techniques. Herein, we report the development of three paper based colorimetric sensors for the on-spot quantitative detection of the levels of fluoride (F−), lead (Pb2+), and pH in drinking water. The variations in the color-intensities of the paper sensors with the variation in the concentration of fluoride (F−), lead (Pb2+), and pH were converted into electronic signals to enable the development of a point-of-care-testing (POCT) device. For this purpose, initially, the sensors were characterized and calibrated with known concentrations of contaminates before successful validations with real samples. Thereafter, the sensors were illuminated with a light emitting diode from one side while the transmitted rays passing through the sensor was captured by a light dependent resistor (LDR) from the other side. The variation in the color intensity of the paper-sensor with the contaminant loading was found linearly varying with the resistance of the LDR. The sensor was then translated into a specific, stable and user friendly POCT device, which enabled inexpensive spot detection of pollutants in drinking water.

Pancreatic disorders, originating from alcoholism or other sources, have grown into an epidemic in modern society. It is now well known that regular monitoring of -amylase and lipase activities in the blood serum is crucial to judge the condition of the pancreas and assess progress in treatment. However, there is hardly any gadget available at the user's site which can provide guidance on pancreatic health. The present spectroscopic techniques used in pathological laboratories are not only costly but also involve significant time and expertise for testing and analysis. In view of the above, we report the development of a paper-based chemiresistive sensor for the quantitative detection of lipase concentration in human serum. The sensor can be integrated with a point-of-care-testing (POCT) device to provide affordable primary healthcare related to the pancreatic disorders. The hydrolysis of olive oil with lipase present in human blood serum results in the generation of H ions on a reduced graphene oxide-coated paper substrate. A linear variation in electrical resistance with lipase loading in serum configured the working principle of the sensor. The sensors are characterized and calibrated with known concentrations of lipase followed by successful validation with real-life human serum samples. The lower cost, simpler operation, smaller response time, and easy disposability of the sensors ensure their suitability to evolve into a POCT kit for the regular monitoring of pancreatic health at the user's end.

In the following work we have identified and isolated transcripts induced upon treatment of jute (JRC 412) seeds with as elicitors through the differential display analysis. We have tried an approach of pre-treatment of jute seeds with CaCl2 (calcium chloride), CaNP (hydroxyapatite nano-particle) and BABA (β-amino butyric acid) as elicitors to study the changes in both biochemical parameters and transcriptomic changes. Rice was chosen as the biological replicate model for the enzymatic changes involved. Several genes including the Late Embryogenesis Abundant protein, Dehydration Responsive Element along with the biochemical markers like proline and peroxidase associated with the priming and Systemic Acquired Resistance of the jute seedlings were found to be up-regulated under the effect of the elicitors. The significance of the isolated differentially expressed genes has been discussed in the light of these findings and there was distinct similarity in the enzyme profile that suggested that biosynthesis and control of proline levels in stressed plants was central to the survival strategy.

Activity of α-amylase enzyme in human serum indicates the onset of pancreatitis, mumps, cancer, stress, and depression. Herein we design and develop a biosensor for the point-of-care-testing (POCT) of α-amylase concentration in serum. The biosensor is composed of a glass substrate coated with an electrically conducting poly-aniline-emeraldine-salt (PANI-ES) film covered with starch-coated gold nanoparticles (SAuNPs). Addition of different dosage of α-amylase on the biosensor selectively depletes starch stabilized on the SAuNPs, which changes the electrical resistance of the sensor. The change in electrical resistance show a nearly linear correlation with the concentration of α-amylase in buffer, which helps the detection of unknown α-amylase activity in the blood serum. The biosensor responds in a specific manner owing to the use of selective enzymatic chemical reaction between α-amylase and starch. The pathways to SAuNP formation on PANI-ES, time-dependent starch digestion with α-amylase, and the subsequent variation in electrical response was characterized to uncover the sensing mechanism. The chloride ions and the AuNPs present catalyse the starch-amylase reaction on the PANI surface to enable a sensitive detection of α-amylase in serum (25 – 100 U/l) at a quick response time of ~60 s. Integration of the biosensor with the built-in sourcemeter and a real time display help an immediate presentation of α-amylase level in the serum, comparable to the clinically approved methodologies.

Nickel-based super alloy (such as Inconel) is widely used in aerospace, nuclear, and chemical industries because of its excellent mechanical and chemical properties at elevated temperatures. Inconel comes under the category of "diffcult-to-cut" materials. In the present case, an experimental investigation on assessing machining performance during EDM of Inconel 601 has been delineated herein. Attempt has been made on evaluating optimal machining parameters setting to achieve satisfactory machining yield. A Box–Behnken design of response surface methodology has been adopted to es timate the effect of machining para meters on the response. Experiments have been carried out by varying gap voltage, peak current, pulse-on time (each varied at three discrete levels) to examine the extent of machining performance in terms of material removal rate using Brass and Graphite electrode.

We report the fabrication of a paper-sensor for quantitative detection of α-amylase activity in human blood serum. Pieces of filter papers were coated with starch-iodine solution leading to an intense blue coloration on the surface. Dispensing α-amylase solution on the starch-iodine coated paper reduced the intensity of the color because of starch-hydrolysis catalyzed by amylase. The variation in the intensity of the color with the concentration of amylase was estimated in three stages: (i) initially, the paper-surface was illuminated with a light emitting diode, (ii) then, the transmitted (reflected) rays emitted through (from) the paper were collected on a photoresistor, and (iii) the variations in the electrical resistance of the photoresistor were correlated with the amylase concentration in analyte. The resistance of photoresistor decreased monotonically with an increase in amylase concentration because the intensity of the reflected (transmitted) rays collected from (through) the paper increased with reduction in the color intensity on the paper surface. Since a specific bio-reaction was employed to detect the activity of amylase, the sensor was found to be equally efficient in detecting unknown quantities of amylase in human blood serum. The reported sensor has shown the potential to graduate into a point-of-care detection tool for α-amylase.

It is well known that the human being is a complex system made up of cells, tissues, organs and organ systems that function together to maintain the health. The coordinated physiological processes maintain most of the steady states in an organism, not at a fixed value, but within a narrow, relatively constant range, known as homeostasis [1]. Recently, a non-invasive measurement of human biopotentials has been proposed in order to provide information about the condition of the human system as a whole [2]. In the present work, a signal conditioning circuit has been developed to enhance the signal level of the measured biopotentials so as to achieve better resolution. This is expected to enable a more reliable and detailed analysis of the acquired signals.

Background Viral infections, particularly the infections caused by herpes simplex virus (HSV), represent one of the most serious public health concerns globally because of their devastating impact. The aim of this study was to evaluate the antiviral potential of methanolic crude extract of an ethnomedicine Mallotus peltatus, its active fraction and pure compound, against HSV-1 F and HSV-2 G. Result The cytotoxicity (CC50, the concentration of 50% cellular toxicity), antiviral effective concentration (EC50, the concentration required to achieve 50% protection against virus-induced cytopathic effect), plaque reduction and the selectivity index (SI, the ratio of CC50 and EC50) was determined. Results showed that the crude methanolic extract of M. peltatus possessed weak anti-HSV activity. In contrast, the active fraction A and isolated ursolic acid from fraction A exhibited potent antiherpesvirus activity against both HSV-1 (EC50 = 7.8 and 5.5 μg/ml; SI = 22.3 and 20) and HSV-2 (EC50 = 8.2 and 5.8 μg/ml, and SI = 21.2 and 18.97). The fraction A and isolated ursolic acid (10 μg/ml) inhibited plaque formation of HSV-1 and HSV-2 at more than 80% levels, with a dose dependent antiviral activity, compared to acyclovir. The time response study revealed that the anti-HSV activity of fraction A and isolated ursolic acid is highest at 2–5 h post-infection. Moreover, the time kinetics study by indirect immunofluorescence assay showed a characteristic pattern of small foci of single fluorescent cells in fraction A- treated virus infected cells at 2 h and 4 h post-infection, suggesting drug inhibited viral dissemination. Further, the PCR study with infected cell cultures treated with fraction A and isolated ursolic acid at various time intervals, failed to show amplification at 48–72 h, like acyclovir treated HSV-infected cells. Moreover, fraction A or isolated ursolic acid showed no interaction in combination with acyclovir. Conclusion This study revealed that bioactive fraction A and isolated ursolic acid of M. peltatus has good anti-HSV activity, probably by inhibiting the early stage of multiplication (post-infection of 0–5 h), with SI value of 20, suggesting its potential use as anti-HSV agents.