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Kluyvera spp. is a Gram-negative bacillus and was discovered by Kluyver and van Niel, included in the family Enterobacteriaceae. We report two cases of urinary tract infection due to Kluyvera ascorbata. The first case was a 61-year-old male, who was admitted to the nephrology department of the hospital with chief complaints of fever with chills from 1 week, abdominal pain, and difficulty in passing urine. The second case was a 29-year-old female, who presented to the medicine department of the hospital with a 4-day history of fever with associated chills and rigor, abdominal pain, chills, and weakness. These patients were treated with nitrofurantoin given orally for 7 days they recovered completely. This case series highlights the role of K. ascorbata, and in general of Kluyvera species, as an unusual but potentially dangerous pathogen, the need for prompt identification to determine potential pathogenicity in the immunocompetent as well as in the immunocompromised host, and to assess the efficacy of antibiotics such as carbapenems and nitrofurantoin.

The spread of the Coronavirus disease 2019 (COVID-19) has impacted human life severely since November 2019. The urban centers in the world, especially, were highly affected by the diseases. Several socioeconomic and environmental factors probably enhanced the spread of the pandemic and consequent mortality. Many studies examining environmental factors, such as air quality, in urban centers indicate the roles of those factors in the spread of diseases and consequent mortality. However, other socioeconomic factors that directly or indirectly elevate the mass death of people are seldom studied. The present study explores the socioeconomic factors and air quality influencing COVID-19 deaths in urban India. We randomly selected 19 Indian cities and collected each city’s socioeconomic and air quality data from reliable and open sources. The data were analyzed using multivariate data analysis techniques using R statistics. The results showed significant positive relationships, population, and total area of the urban centers.

The highly conserved Notch pathway, a pillar of juxtacrine signaling, orchestrates intricate intercellular communication, governing diverse developmental and homeostatic processes through a tightly regulated cascade of proteolytic cleavages. This pathway, culminating in the migration of the Notch intracellular domain (NICD) to the nucleus and the subsequent activation of downstream target genes, exerts a profound influence on a plethora of molecular processes, including cell cycle progression, lineage specification, cell–cell adhesion, and fate determination. Accumulating evidence underscores the pivotal role of Notch dysregulation, encompassing both gain and loss-of-function mutations, in the pathogenesis of numerous human diseases. This review delves deep into the multifaceted roles of Notch signaling in cellular dynamics, encompassing proliferation, differentiation, polarity maintenance, epithelial–mesenchymal transition (EMT), tissue regeneration/remodeling, and its intricate interplay with other signaling pathways. We then focus on the emerging landscape of Notch aberrations in gynecological pathologies predisposing individuals to infertility. By highlighting the exquisite conservation of Notch signaling in Drosophila and its power as a model organism, we pave the way for further dissection of disease mechanisms and potential therapeutic interventions through targeted modulation of this master regulatory pathway.

Background: Novel coronavirus disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shows various respiratory symptoms of varying severity. However, the virus can affect any organ in the body. Understanding about the oral manifestations would be helpful in early diagnosis and treatment of this disease. Aim: The aim of the study was to understand the oral manifestations associated with SARS-CoV-2 infection using a self-designed questionnaire. Objectives: To understand the impact of SARS-CoV-2 infection in oral cavity so that early treatment can be provided. Materials and Methods: The study was done on 100 COVID-19-positive dental students above 18 years with positive RT-PCR reports. Participants were asked to fill the questionnaire after doing a self-examination of the oral cavity throughout the course of infection. Result: Fifty-five per cent of patients experienced oral manifestations during the course of infection. Various oral manifestations including xerostomia, dysphagia, altered taste sensation, burning sensation, redness of oral mucosa, halitosis, blisters, and white patches were reported by the participants. Conclusion: Various oral manifestations are seen in COVID-19-positive patients hence SARS-CoV-2 infection has a significant effect on oral epithelium.

Background: Chronic periodontitis is the most common dental disease reported globally as well as in India. Periodontal pathogens are usually seen in samples of gingival tissues, crevicular blood, GCF (gingival crevicular fluid), and dental plaque. Apart from the conventional mechanical treatment, laser disinfection is a recent advancement that change shows greater efficacy in reducing the disease progression and changing the bacterial flora. Aim: The present study aimed to assess the Immediate response of diode laser on the microbial load in subjects with chronic Periodontitis as assessed in saliva, crevicular blood, and GCF (gingival crevicular fluid) samples. Materials and Methods: The study recruited 90 subjects with chronic periodontitis. For split-mouth fashion, the mouth, of each participant was divided into two halves and was divided into two groups randomly. Group I (test group) subjects underwent laser disinfection (970 ± 15 nm). Group II subjects served as controls and underwent saline irrigation. For all participants, crevicular blood, saliva, and GCF samples were collected before and immediately following disinfection for microbial analysis. Results: Microbial load reduction was seen in both groups following treatment. However, a significantly higher reduction was seen in the test group with laser disinfection. Compared to the crevicular blood sample, a greater reduction was seen in saliva and GCF samples. Conclusion: The present study concludes that Diode Laser (970 ± 15 nm) application shows an immediate reduction of the bacterial load in subjects with chronic periodontitis.

Background: Achromobacter spp. is a rare nosocomial pathogen known to cause many serious infections like bloodstream infection, osteomyelitis, meningitis, urinary tract infections, corneal ulceration, peritonitis, and endocarditis. Materials and Methods: In this study, we retrospectively analyzed all the bacteriological sample records from the bacteriology database from January 2021 to December 2021 to determine the prevalence of Achromobacter spp. Result: Nine patients with Achromobacter xylosoxidans causing bacterial infection were identified, from whom five blood samples, two pus, one respiratory sample, one central venous pressure (CVP), and one cerebrospinal fluid were found positive for Achromobacter spp., among whom one was a 6-year-old patient having the same organism from two different body sites—CVP line and cerebrospinal fluid. Five patients had coinfection with another organism, whereas acute kidney injury was the most common comorbidity. Conclusion: In our single-center experience, approximately 50% of the cases with Achromobacter denitrificans bacteremia suffered from chronic kidney disease and had a history of antibiotic therapy, hospitalization, and the presence of devices. Active research on rising trends of Achromobacter spp. needs to be promoted.

First off, humans now enjoy a higher standard of living thanks to contemporary technology. But shopping has become a world unto itself in the modern day. With its cutting-edge technology, the “Smart Shopping Trolley Using RFID and ZigBee” seeks to completely transform the shopping experience. With Radio-Frequency Identification (RFID), ZigBee communication, and a Hc-05 Bluetooth module, this cutting-edge cart makes shopping easy and productive. Customers may easily add items with RFID tags to the cart thanks to RFID technology. There is no longer a need for human scanning or checkout lines because the system automatically recognizes and records the items [9]-[10]. The ZigBee communication system monitors the items in customers’ carts as they navigate the store and syncs with their mobile phone via a Bluetooth module into the app called Serial Bluetooth terminal which guarantee precise billing.

Underutilized/orphan legumes provide food and nutritional security to resource-poor rural populations during periods of drought and extreme hunger, thus, saving millions of lives. The Leguminaceae, which is the third largest flowering plant family, has approximately 650 genera and 20,000 species and are distributed globally. There are various protein-rich accessible and edible legumes, such as soybean, cowpea, and others; nevertheless, their consumption rate is far higher than production, owing to ever-increasing demand. The growing global urge to switch from an animal-based protein diet to a vegetarian-based protein diet has also accelerated their demand. In this context, underutilized legumes offer significant potential for food security, nutritional requirements, and agricultural development. Many of the known legumes like Mucuna spp., Canavalia spp., Sesbania spp., Phaseolus spp., and others are reported to contain comparable amounts of protein, essential amino acids, polyunsaturated fatty acids (PUFAs), dietary fiber, essential minerals and vitamins along with other bioactive compounds. Keeping this in mind, the current review focuses on the potential of discovering underutilized legumes as a source of food, feed and pharmaceutically valuable chemicals, in order to provide baseline data for addressing malnutrition-related problems and sustaining pulse needs across the globe. There is a scarcity of information about underutilized legumes and is restricted to specific geographical zones with local or traditional significance. Around 700 genera and 20,000 species remain for domestication, improvement, and mainstreaming. Significant efforts in research, breeding, and development are required to transform existing local landraces of carefully selected, promising crops into types with broad adaptability and economic viability. Different breeding efforts and the use of biotechnological methods such as micro-propagation, molecular markers research and genetic transformation for the development of underutilized crops are offered to popularize lesser-known legume crops and help farmers diversify their agricultural systems and boost their profitability.

Horticultural production is a vital catalyst for economic growth, yet insect infestations reduce horticultural crop yield and quality. Pesticides and other pest control methods are used during planting to eliminate pests that cause direct and indirect losses. In such situations, endophytic entomo-pathogenic fungi (EEPF) can act as a potential tools for biological control. They protect plants by boosting growth, nutrition, morpho-physiology and salt or iron tolerance. Antixenosis, antibiosis and plant tolerance change insect performance and preferences. EEPF- plant colonisation slows herbivore development, food consumption, oviposition and larval survival. EEPF changes plant physio-chemical properties like volatile emission profile and secondary metabolite production to regulate insect pest defences. EEPF produces chitinases, laccases, amylases, and cellulases for plant defence. Recent studies focused on EEPF species’ significance, isolation, identification and field application. Realizing their full potential is difficult due to insufficient mass production, storage stability and formulation. Genetic-molecular and bioinformatics can help to build EEPF-based biological control systems. Metagenomics helps study microbial EEPF taxonomy and function. Multi-omics and system biology can decode EEPF interactions with host plants and microorganisms. NGS (Next Generation Sequencing), comparative genomics, proteomics, transcriptomics, metabolomics, metatranscriptomics and microarrays are used to evaluate plant-EEPF relationships. IPM requires understanding the abiotic and biotic elements that influence plant-EEPF interaction and the physiological mechanisms of EEPF colonisation. Due to restricted research, there are hundreds of unexplored EEPFs, providing an urgent need to uncover and analyse them.

In this paper, a high gain structure of operational transconductance amplifier is presented. For low voltage operation with improved frequency response bulk driven quasi-floating gate MOSFET is used at the input. Further for achieving high gain the modified self cascode structure is used at the output. Compared to conventional self cascode the modified self cascode structure used provides higher transconductance which helps in significant boosting of gain of the amplifier. The modification is achieved by employing quasi-floating gate transistor which helps in scaling of the threshold which as a result increases the drain-to-source voltage of linear mode transistor thus changing it to saturation. This change of mode boosts the effective transconductance of self cascode MOSFET. The proposed operational transconductance amplifier when compared to its conventional showed improvement in DC gain by 30dB and also the unity gain bandwidth increases by 6 fold. The MOS models used for amplifier design are of 0.18µm CMOS technology at supply of 0.5V.

A novel energy harvester inspired by a winding mechanism which utilises the loading deflection because of vehicle mobility, when it crosses speed breaker is designed and fabricated. The method of energy harvesting using deflection produced by vehicle weight moving on the road can provide a relatively larger and continuous power to the connected load. The developed system in accordance with mechanical motion rectifier has used a method for converting deflection produced by the weight of the vehicle passing over the speed breaker to electrical energy, which resolves the issue of regeneration of lost energy from impulsive vibration. The design approach, power calculation and working mechanism of energy harvester are presented. The dead load application in the lab gives a peak output of 4.6 W and a steady state output of 3.25 W. A sedan vehicle is considered for the realisation of the developed system, which ultimately gives peak output of 18.4 W and a steady-state output of 13 W, which can be utilised to power the typical traffic lights of 12 W along with the street lights. This method of decentralised energy harvesting can lead to a grid-independent traffic signal and control.

Potato, the world's most popular crop is reported to provide a food source for nearly a billion people. It is prone to a number of biotic stressors that affect yield and quality, out of which Potato Virus Y (PVY) occupies the top position. PVY can be transmitted mechanically and by sap-feeding aphid vectors. The application of insecticide causes an increase in the resistant vector population along with detrimental effects on the environment; genetic resistance and vector-virus control are the two core components for controlling the deadly PVY. Using transcriptomic tools together with differential gene expression and gene discovery, several loci and genes associated with PVY resistance have been widely identified. To combat this virus we must increase our understanding on the molecular response of the PVY-potato plant-aphid interaction and knowledge of genome organization, as well as the function of PVY encoded proteins, genetic diversity, the molecular aspects of PVY transmission by aphids, and transcriptome profiling of PVY infected potato cultivars. Techniques such as molecular and bioinformatics tools can identify and monitor virus transmission. Several studies have been conducted to understand the molecular basis of PVY resistance/susceptibility interactions and their impact on PVY epidemiology by studying the interrelationship between the virus, its vector, and the host plant. This review presents current knowledge of PVY transmission, epidemiology, genome organization, molecular to bioinformatics responses, and its effective management.

Modernization of the agricultural production system led to a significant increase in annual food production intended to meet the ever-growing consumer demand. In many countries, most of the food grains produced is stored for contingency and regular supply. These stored grains, in general, are directly or indirectly infested by insects, resulting in severe grain damages and storage losses, thus, causing a threat to food safety and security. Although a variety of insect management options, such as physical, mechanical, biological, and chemical methods, are available, fumigation has been practiced for decades in storage. However, opportunities for fumigation are narrowing after the phase-out of methyl bromide. Besides, safe food and health concerns paved the path for green chemistry and non-chemical management practices. This review includes the list of stored-grain insects and their detection methods. The different management strategies such as the modern storage structures (hermetic and low-pressure storages), modified or controlled storage atmosphere, application of ozone as fumigant, irradiation, and physical options are presented. Further, the details on sustainable biological options, such as semiochemicals, natural enemies, biopesticides, and entomopathogenic nematodes, are supplemented. The use of inert dusts as grain protectant and in combination with the biological entity is included. Studies on alternative fumigants', novel management options, such as molecular biology tools (RNAi and CRISPR) and nanotechnology in stored grain protection, are also highlighted. This review helps the reader to understand the overall factors affecting grain storage and the different options to manage the insects causing storage losses.

In the field of renewable energy, the extraction of parameters for solar photovoltaic (PV) cells is a widely studied area of research. Parameter extraction of solar PV cell is a highly non-linear complex optimization problem. In this research work, the authors have explored grey wolf optimization (GWO) algorithm to estimate the optimized value of the unknown parameters of a PV cell. The simulation results have been compared with five different pre-existing optimization algorithms: gravitational search algorithm (GSA), a hybrid of particle swarm optimization and gravitational search algorithm (PSOGSA), sine cosine (SCA), chicken swarm optimization (CSO) and cultural algorithm (CA). Furthermore, a comparison with the algorithms existing in the literature is also carried out. The comparative results comprehensively demonstrate that GWO outperforms the existing optimization algorithms in terms of root mean square error (RMSE) and the rate of convergence. Furthermore, the statistical results validate and indicate that GWO algorithm is better than other algorithms in terms of average accuracy and robustness. An extensive comparison of electrical performance parameters: maximum current, voltage, power, and fill factor (FF) has been carried out for both PV model.

India's mainstream political discourse is yet to grant genuine space to environmental issues or sustainable development; their priority being economic development. This is despite noteworthy environmental conservation movements and civil society organizations committed to the cause of the environment. Although the country has an array of competent environmental regulations, the environmental sustainability of the development programmes remains the least addressed. The case is not different for the Kerala state, despite its high literacy and human development matching many developed countries. The "Kerala Development Model" has been highly praised for its high-level social development. The apparently well-informed population of the state also outscores most states regarding environmental identity. In this context, this paper explores the Athirappilly hydroelectric Project pursued by the Kerala State Electricity Board Limited and other interest groups. It appears that the contemporary socio-economic-political scenario is urging the state's highly literate and politicized public to a post-nature or post-truth society increasingly engrossed in a consumerist lifestyle at the cost of the unique environmental setup. The present study also highlights how the Kerala population, once dominated by socialistic/liberal ideologies with egalitarian values, has been riding the neoliberal development wave giving little importance to nature and long-term ecological security. [ABSTRACT FROM AUTHOR]

We present a rare case of pathological shaft of femur fracture in an 18-year-old male, with an underlying arteriovenous malformation (AVM). Magnetic resonance imaging showed extensive intraosseous femoral involvement of the vascular malformation, and the canal was deemed to be wide enough for a nail based on the radiograph templates and computed tomography (CT) scan. The patient was given four sessions of stereotactic external beam radiotherapy preoperatively, and closed reduction and internal fixation was performed using a proximal femoral nail without any complications. Radiographic evidence of fracture union was observed at 4 months. Fracture fixation by closed reduction can be either intramedullary fixation or external fixation based on the adequacy of the canal as determined by radiograph and CT scan. Preoperative measures to decrease vascularity such as stereotactic radiotherapy and angiographic embolization can be undertaken to reduce bleeding. A multidisciplinary approach is essential to improve the management of fractures in a patient with AVM.

Background and Aim: Flexible denture base has been gaining attention as a denture base material because of its toxicological safety, comfort, and enhanced aesthetics. Debonding of acrylic teeth from the flexible denture base material has posed a great problem to the clinician and patient. This study aimed at comparing the bond strength between flexible denture base with acrylic teeth having different mechanical modifications. Materials and Methods: Forty mandibular molar teeth (Combination Acry Rock, Badia Polesine (Ro), Italy) were used in four groups for mechanical modifications on the ridge lap area. The groups were group 1 (no mechanical modifications), group 2 (round groove 2 mm in diameter and 2 mm deep on the ridge lap surface), group 3 (horizontal slot 2 mm deep, 2 mm wide, and 4 mm long prepared on the ridge lap surface of the teeth), and group 4 (T-shaped groove 2 mm deep, 2 mm wide, and length of 4 mm mesiodistally and 2 mm buccolingually prepared with a straight fissure bur). The acrylic teeth were attached to a wax block of dimension 1.2cm × 1.2cm × 2.5cm. These were flasked and dewaxed, followed by injection molding with Valplast flexible denture base material. The bond strength was assessed by attaching the specimens to universal testing machine tested under a tensile load at a cross-head speed of 1 mm/min till it fractured. Data obtained were statistically evaluated by one-way analysis of variance and post hoc test. Results: There was significant increase in mean bond strength after various mechanical modifications. Group with T-shaped diatoric hole showed greatest bond strength value compared to other groups. Conclusion: Incorporating mechanical retentive features with increased surface area and undercut can improve the bonding of acrylic teeth to flexible denture base.

We report on the use of silver nanodisks (AgNDs), having a diameter of 50 ± 8 nm and a thickness of 8 ± 2 nm, as electrochemical labels for the detection of a model metalloimmunoassay for the heart failure biomarker NT-proBNP. The detection method is based on an electrochemically activated galvanic exchange (GE) followed by the detection of Ag using anodic stripping voltammetry (ASV). The AgNDs labels are superior to Ag nanocubes and Ag nanospheres in terms of the dynamic range for both the model and NT-proBNP metalloimmunoassays. The linear dynamic range for the model composite is 1.5 to 30.0 pM AgNDs. When AgND labels are used for the NT-proBNP assay, the dynamic range is 0.03–4.0 nM NT-proBNP. The latter range fully overlaps the risk stratification range for heart failure from 53 pM to 590 pM. The performance improvement of the AgNDs is a result of the specific GE mechanism for nanodisks. Specifically, GE is complete across the face of the AgNDs, leaving behind an incompletely exchanged ring structure composed of both Ag and Au.

In this article, we compare linear sweep anodic stripping voltammetry (LASV) and square-wave anodic stripping voltammetry (SWASV) for detection of a nano metalloimmunoassay. Two separate immunoassays were examined: a model assay, based on interactions between antibodies, and a sandwich assay for the heart failure marker NT-proBNP. In both cases, one antibody is linked to a magnetic microbead, and one is linked to a spherical Ag nanoparticle label. Electrochemical detection is carried out on a paper device. The three analytical figures of merit studied were the precision of the measurements, the calibration sensitivity, and the limit of detection (LOD). For the NT-proBNP assay, the results show that after optimization of the pulse amplitude and frequency of the potential input for SWASV, the detection efficiency is substantially higher compared to LASV. Specifically, the calibration sensitivity increased by up to ~40 fold, the average coefficient of variation decreased by ~40%, and the (LOD) decreased to 300.0 pM. Finally, for a model immunoassay, a ~10-fold decrease in the LOD was observed for SWASV compared to LASV.

Regulatory approvals for the marketing of medicinal products authorize medical practitioners to prescribe drugs to a group of patients that are defined within the license of the medicinal product. However, such prescriptions are carried out in a controlled manner. Prior to being approved, the medicinal product will have been evaluated in a population pool containing fewer than 5,000 patients and in a predesigned environment where several factors may be lacking, such as the absence of women of childbearing potential, geriatric patients and paediatric patients. Therefore, it is not surprising that several major adverse drug reactions are detected only when the product has been prescribed to the general population. National and international regulatory bodies have devised systems for monitoring medicinal products after marketing, commonly known as postmarketing surveillance systems. Postmarketing surveillance refers to the process of monitoring the safety of drugs once they reach the market, after the successful completion of clinical trials. The primary purpose for conducting postmarketing surveillance is to identify previously unrecognized adverse effects as well as positive effects. The Yellow Card scheme, practiced in the United Kingdom and the Canada Vigilance Program adopted in the Canadian jurisdiction, are two of the most successful postmarketing surveillance systems implemented across the world. Therefore, this article intends to discuss postmarketing surveillance and its role in the context of the United Kingdom and Canadian jurisdictions with a view on presenting key aspects and measures that are employed for operating an efficient postmarketing surveillance system in regulated markets.

Background: The present study was conducted to assess age of adults by using Olze′s method and also to estimate the efficacy of Olze′s method in south Indian population. Materials and Methods: The present study comprised of 25 subjects ranging from 12 to 26 years from Mangalore. Dental age was assessed by using the Olze′s method based on upper and lower third molar. Panoramic radiographs were taken for the same. The obtained data were analyzed by using paired t-test, intraclass correlation coefficient, and regression analysis using Statistical Package for Social Sciences (SPSS) 13 software for statistical analysis. Results: Average chronological age was 16.11. Average age estimated by Olze′s method was 16.05. The intraclass correlation coefficient between the two methods showed excellent agreement between the two. Statistical analysis indicated that there is no significant difference between chronological age and age obtained by Olze′s method. Conclusion: Olze′s method has been experimented by many authors. An attempt was made to apply original method in our population and check its reliability. The present study indicated that, Olze′s method was reliable for age estimation in our sample. This method of age estimation was accurate in both males and females.

Knowing the variations in the general rainfall pattern of a river basin is vital to understanding the hydrological cycle and water budget of the basin. The present study examined the general rainfall pattern in Bharathapuzha River basin of Malabar coast of Kerala, using monthly rainfall data for 34 years collected from 28 rain gauge stations. We used Mann-Kendal's rank correlation statistics and wavelet analysis to examine the trend of rainfall. The annual rainfall, southwest monsoon, and pre-monsoon rainfall of the basin shows a significant decrement towards the later years of the study. We assume that the global climate and the local environmental changes are the chief factors for the variation. Copyright © 2010 Royal Meteorological Society [ABSTRACT FROM AUTHOR]

The article presents a study which aims to provide a checklist of birds found on the Pallikaranai wetlands in Chennai, India. The study conducted two field surveys for 10 and seven days from February to August 2007 in the region's marsh lands, during which the birds were recorded by calls or direct sighting. Results show that the region had 101 species of migratory and resident birds with the black-winged stilts and little grebes being the most numerous ones.

The article offers information about the COP-15 climate change conference by the Conference of Parties (COP) to be held in Copenhagen, Denmark in December 2009.