7 results on '"Nigl, K."'
Search Results
2. Ernährung und chronisch entzündliche Darmerkrankungen – ein Konsensus der Arbeitsgruppe chronisch entzündliche Darmerkrankungen der Österreichischen Gesellschaft für Gastroenterologie und Hepatologie (Austrian Guidelines for nutrition in IBD)
- Author
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Fuchssteiner, H., additional, Nigl, K., additional, Mayer, A., additional, Kristensen, B., additional, Platzer, R., additional, Brunner, B., additional, Weiß, I., additional, Haas, T., additional, Benedikt, M., additional, Gröchenig, H., additional, Eisenberger, A., additional, Hillebrand, P., additional, Reinisch, W., additional, and Vogelsang, H., additional
- Published
- 2014
- Full Text
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3. 16S rDNA-based identification of bacteria from conjunctival swabs by PCR and DGGE fingerprinting
- Author
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Schabereiter-Gurtner C, Maca S, Rölleke S, Nigl K, Lukas J, Hirschl A, Lubitz W, and Talin Barisani-Asenbauer
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DNA, Bacterial ,Electrophoresis, Agar Gel ,Bacteria ,Sequence Analysis, DNA ,DNA Fingerprinting ,DNA, Ribosomal ,Polymerase Chain Reaction ,Bacterial Typing Techniques ,Conjunctivitis, Bacterial ,RNA, Bacterial ,RNA, Ribosomal, 16S ,Humans ,Conjunctiva ,DNA Primers - Abstract
Establishment of a new molecular biology technique for the identification of multiple bacteria from the ocular environment, which can be applied supplementarily to cultivation in cases of severe bacterial infections.From 60 human conjunctivae (29 with purulent and 31 with nonpurulent conjunctivitis), swabs were taken and DNA was extracted. Fragments of 200 bp, spanning the V3 region of the eubacterial 16S rDNA, were amplified by polymerase chain reaction (PCR) and separated by denaturing gradient gel electrophoresis (DGGE). For phylogenetic identification, DGGE bands were excised and directly sequenced, or 16S rDNA clone libraries were constructed and clones were screened by DGGE. Sequences were compared with sequences of known bacteria listed in the EMBL database. Furthermore, the results were compared with results obtained from conventional cultivation.16S rDNA could be amplified from 25 of 29 investigated swabs taken from purulent conjunctivitis eyes and from 2 of 31 investigated swabs taken from nonpurulent conjunctivitis eyes. Sixteen samples showed monomicrobial and 11 samples showed polymicrobial infections. The following genera (n is number of samples) were detected: Staphylococcus (n = 8), Corynebacterium (n = 7), Propionibacterium (n = 7), Streptococcus (n = 6), Bacillus (n = 2), Acinetobacter (n = 3), Pseudomonas (n = 3), Proteus (n = 1), and Brevundimonas (n = 1). Four sequences could not be identified to the genus level. They had highest sequence similarities both to sequences of Pantoea and Enterobacter (n = 1), Kingella and Neisseria (n = 1), Serratia and Aranicola (n = 1), and Leuconostoc and Weissella (n = 2), respectively. Culture was only positive for coagulase-negative staphylococci (n = 9), Corynebacteria (n = 3), Staphylococcus aureus (n = 1), Streptococcus sp. (n = 1), Proteus sp. (n = 1), Klebsiella oxytoca (n = 1), and Pseudomonas aeruginosa (n = 1). In total, 45% of the 60 analyzed conjunctival swabs were PCR positive, whereas only 22% were culture positive. No sample positive by culture gave negative results by PCR.16S rDNA sequence analyses and DGGE fingerprinting are appropriate methods for the detection and identification of monomicrobial as well as polymicrobial ocular infections of bacteria that might not be detected by conventional cultivation.
- Published
- 2001
4. Power-Up for Mucoadhesiveness: Two Generations of Thiolated Surfactants for Enhanced Sticky Nanoemulsions.
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To D, Kali G, Haddadzadegan S, Jörgensen AM, Nigl K, Ricci F, and Bernkop-Schnürch A
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- Animals, Swine, Surface-Active Agents pharmacology, Stearates, Sulfhydryl Compounds chemistry, Cysteine chemistry, Drug Delivery Systems methods
- Abstract
Nanoemulsions can be tuned toward enhanced gastro-intestinal retention time by incorporating thiolated surfactants into their surface. Tailoring the chemical reactivity of the thiol headgroup has major influence on mucoadhesive features of the nanoemulsion. Two generations of thiolated surfactants were synthetically derived from PEG-40-stearate featuring either a free thiol group or an S-protected thiol group. The surfactants were characterized regarding critical micelle concentration (CMC), hemolytic activity, and cytotoxicity. Subsequently, they were incorporated into nanoemulsions and the resulting nanoemulsions were characterized regarding particle size, polydispersity index (PDI), zeta potential, and time-dependent stability. Afterward, mucosal interactions as well as mucoadhesion on porcine intestinal mucosa were investigated. Successful synthesis of Cysteine-PEG-40-stearate (CYS-PEG-40-stearate) and MNA-Cysteine-PEG-40-stearate (MNA-CYS-PEG-40-stearate) was confirmed by
1 H NMR spectroscopy. Both chemical modifications led to slightly elevated CMC values while preserving low cytotoxicity and hemotoxicity. Incorporation into nanoemulsions had minor influence on overall physical particle characteristics, while interactions with mucus and mucoadhesiveness of the nanoemulsions were drastically improved resulting in the rank order PEG-40-stearate < CYS-PEG-40-stearate < MNA-CYS-PEG-40-stearate. Accordingly, thiolated surfactants, especially S-protected derivatives, are versatile tools to generate highly mucoadhesive nanoemulsions.- Published
- 2023
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5. Intraindividual comparative analysis of capsule opacification after implantation of 2 single-piece hydrophobic acrylic intraocular lenses models: Three-year follow-up.
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Kahraman G, Amon M, Ferdinaro C, Nigl K, and Walch M
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- Acrylic Resins, Aged, Aged, 80 and over, Capsule Opacification diagnosis, Double-Blind Method, Female, Follow-Up Studies, Humans, Hydrophobic and Hydrophilic Interactions, Male, Middle Aged, Phacoemulsification, Prospective Studies, Prosthesis Design, Anterior Capsule of the Lens pathology, Capsule Opacification epidemiology, Lens Implantation, Intraocular, Lenses, Intraocular, Posterior Capsule of the Lens pathology, Postoperative Complications
- Abstract
Purpose: To compare the 3-year postoperative anterior (ACO) and posterior (PCO) capsule opacification and the level of anterior capsule retraction after implantation of 2 single-piece hydrophobic acrylic intraocular lens (IOL) models., Setting: Hospital of St. John of God, Vienna, Austria., Design: Comparative randomized controlled double-blind clinical trial., Methods: Eyes with bilateral cataract were evaluated. Each patient had an Acrysof SA60AT (interrupted optic edge) IOL implanted in 1 eye (Group A) and a Tecnis ZCB00 (continuous optic edge) IOL implanted in the fellow eye (Group B). One and 3 years postoperatively, PCO was evaluated using Evaluation of Posterior Capsule Opacification software and the ACO level and capsule-retraction level were evaluated and graded subjectively., Results: The study evaluated 100 eyes of 50 patients ranging from 61 to 80 years. Postoperatively, there were no statistically significant differences in PCO between Group A and Group B at 1 year (0.06 ± 0.12 [SD] and 0.07 ± 0.13, respectively; P = 4.35) or 3 years (0.23 ± 0.36 and 0.22 ± 0.32, respectively; P = .66). In Group A and Group B, ACO was present in 18.0% of eyes and 2.7% of eyes, respectively, at 1 year (P = .03) and in 92.0% and 24.0%, respectively, at 3 years (P < .01). Capsule phimosis (18.0% at 1 year; 30.0% at 3 years) and glistenings (66.0% at 1 year; 86.0% at 3 years) were observed in Group A only., Conclusion: Both IOLs had similarly low PCO rates 3 years postoperatively, although more ACO and capsule retraction were observed in eyes with the interrupted optic edge IOL., (Copyright © 2015 ASCRS and ESCRS. Published by Elsevier Inc. All rights reserved.)
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- 2015
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6. Anterior and posterior capsular opacification with the Tecnis ZCB00 and AcrySof SA60AT IOLs: a randomised intraindividual comparison.
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Kahraman G, Schrittwieser H, Walch M, Storch F, Nigl K, Ferdinaro C, and Amon M
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- Acrylic Resins, Aged, Aged, 80 and over, Biocompatible Materials, Capsule Opacification diagnosis, Double-Blind Method, Female, Humans, Male, Middle Aged, Phacoemulsification, Prospective Studies, Prosthesis Design, Treatment Outcome, Anterior Capsule of the Lens pathology, Capsule Opacification etiology, Lens Implantation, Intraocular, Lenses, Intraocular, Posterior Capsule of the Lens pathology, Postoperative Complications
- Abstract
Purpose: To evaluate and compare the 1-year postoperative levels of posterior capsule opacification (PCO) as well as the level of anterior capsule retraction and opacification (ACO) after implantation of two single-piece hydrophobic acrylic intraocular lens (IOL) models., Methods: Randomised, controlled, prospective and double-blind study including 148 eyes of 74 patients (age range, 61-80 years) with bilateral senile cataract. Each patient underwent surgery with implantation of an AcrySof SA60AT (Group A) in one eye and Tecnis ZCB00 (Group B) in the fellow eye by the same experienced surgeon. Lens allocation to the first or second operated eye followed a randomisation process. At 12 months postoperatively, the PCO level was evaluated with the Evaluation of Posterior Capsule Opacification software, whereas the level of ACO and capsule retraction was evaluated and graded subjectively., Results: All surgeries were uneventful. The mean PCO scores were 0.10 ± 0.17 and 0.21 ± 0.89 in Groups A and B, respectively, with no statistically significant differences between the groups (p=0.47). No significant correlation was found between the PCO scores in Groups A and B (r=0.16, p=0.17). Regarding ACO, it was present in 15 eyes (20.3%) and two eyes (2.7%) in Groups A and B, respectively (p=0.37). Capsular phimosis (13 eyes, 17.6%) was only observed in Group A., Conclusions: Both IOLs had a similar low rate of PCO 1 year after surgery. However, there seems to be a difference in the anterior capsule behaviour between both IOL models.
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- 2014
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7. [Nutrition and IBD-Consensus of the Austrian Working Group of IBD (Inflammatory Bowel Diseases) of the ÖGGH].
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Fuchssteiner H, Nigl K, Mayer A, Kristensen B, Platzer R, Brunner B, Weiß I, Haas T, Benedikt M, Gröchenig HP, Eisenberger A, Hillebrand P, Reinisch W, and Vogelsang H
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- Austria, Humans, Inflammatory Bowel Diseases complications, Inflammatory Bowel Diseases diagnosis, Malnutrition diagnosis, Malnutrition etiology, Diet Therapy standards, Gastroenterology standards, Inflammatory Bowel Diseases diet therapy, Malnutrition diet therapy, Nutrition Policy, Practice Guidelines as Topic
- Abstract
This is a consensus of the Austrian working group of IBD (inflammatory bowel diseases) of the ÖGGH on nutrition in IBD. Malnutrition should be assessed in case of IBD (in 20 - 70 % of Crohn's patients) and weight loss(> 5 % within 3 months) or nutritional deficiencies or after extensive bowel resection and afterwards also treated. Malnutrition should be treated with medical therapy of IBD and also adequate - as far as possible - with oral nutritional therapy particularly because of reduced life quality, risk of opportunistic infections, osteopenia/osteoporosis, longer hospitalisations and higher mortality. Iron homeostasis, serum levels of Vitamin B12- and folic acid, 25-hydroxyvitamin D and zinc should be checked. Therapy with enteral liquid diets is only indicated as therapy of first choice in children and adolescents, but only in rare situations in adults with IBD. There is - up to now - no proven oral diet for maintenance of remission in IBD. Probiotics as E. coli Nissle could be used as alternative to mesalazine for maintenance of remission in patients with ulcerative colitis. A specific dietary counselling is mandatory in patients with ileostoma or short bowel syndrome. Malnutrition of short bowel patients is particularly dependent on the function and length of the remaining bowel, therefore the most effective medical therapy should be administered., (© Georg Thieme Verlag KG Stuttgart · New York.)
- Published
- 2014
- Full Text
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