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Your search keyword '"Nielsen, Lone Gilmor"' showing total 7 results
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7 results on '"Nielsen, Lone Gilmor"'

3. Genome sequence of an unknown subtype of hepatitis C virus genotype 6:Another piece for the taxonomic puzzle

Author

Pedersen, Martin Schou, Mollerup, Sarah, Nielsen, Lone Gilmor, Jenssen, Håvard, Bukh, Jens, Schønning, Kristian, Pedersen, Martin Schou, Mollerup, Sarah, Nielsen, Lone Gilmor, Jenssen, Håvard, Bukh, Jens, and Schønning, Kristian

Abstract

The surveillance and correct subtyping of hepatitis C virus strains require available and up-to-date publicly available reference genomes. Here, we present the complete open reading frame sequence of a hepatitis C virus genotype 6 strain of an unknown subtype that was discovered during routine subtyping of patients in the clinic.

Published
2019

5. Emergence of a vancomycin-variable Enterococcus faecium ST1421 strain containing a deletion in vanX

Author

Hansen, Thomas Arn, Pedersen, Martin Schou, Nielsen, Lone Gilmor, Ma, Chih Man German, Søes, Lillian Marie, Worning, Peder, Østergaard, Christian, Westh, Henrik, Pinholt, Mette, Schønning, Kristian, Hansen, Thomas Arn, Pedersen, Martin Schou, Nielsen, Lone Gilmor, Ma, Chih Man German, Søes, Lillian Marie, Worning, Peder, Østergaard, Christian, Westh, Henrik, Pinholt, Mette, and Schønning, Kristian

Abstract

Background: Primary screening for VRE with PCR directed against vanA allowed identification of vanA! samples from which VRE could not be isolated when selective culture methods were used. From such a sample a vancomycin-susceptible, vanA! Enterococcus faecium, Efm-V1511, was isolated, when vancomycin selection was not used during culture. Similar isolates with variable susceptibility to vancomycin were obtained in the following months. Objectives: To characterize Efm-V1511 and investigate the causes of variable susceptibility to vancomycin. Methods: All strains were sequenced using Illumina technology. Plasmids containing vanA were reconstructed by scaffolding to known plasmids or plasmids were sequenced using Oxford Nanopore MinION. Derived structures were verified by PCR and sequencing. Furthermore, selected vanA! vancomycin-susceptible isolates were passaged in the presence of vancomycin and vancomycin-resistant variants obtained were sequenced. Results: Efm-V1511 belonged to ST1421 and contained a 49 696 bp plasmid pHVH-V1511 carrying a Tn1546-derived genetic element. Within this element vanX was truncated by a 252 bp 30 deletion explaining the susceptibility of Efm-V1511. Between March 2016 and April 2017, 48 isolates containing pHVH-V1511 were identified. All were ST1421. In isolates resistant to vancomycin, resistance could be attributed to changes in ddl disrupting gene function sometimes accompanied by changes in vanS, increased pHVH-V1511 copy number or the existence of an additional vanA-containing plasmid encoding a functional vanX. Conclusions: E. faecium carrying pHVH-V1511 is capable of nosocomial transmission and may develop clinical resistance to vancomycin. Strains may not be detected using standard culture methods for VRE.

Published
2018

7. Emergence of a vancomycin-variable Enterococcus faecium ST1421 strain containing a deletion in vanX.

Author

Hansen TA, Pedersen MS, Nielsen LG, Ma CMG, Søes LM, Worning P, Østergaard C, Westh H, Pinholt M, and Schønning K

Subjects
Anti-Bacterial Agents pharmacology, Cross Infection microbiology, Cross Infection transmission, Humans, Microbial Sensitivity Tests, Plasmids genetics, Polymerase Chain Reaction, Sequence Analysis, DNA, Bacterial Proteins genetics, Enterococcus faecium drug effects, Enterococcus faecium genetics, Gene Deletion, Serine-Type D-Ala-D-Ala Carboxypeptidase genetics, Vancomycin pharmacology, Vancomycin Resistance genetics
Abstract

Background: Primary screening for VRE with PCR directed against vanA allowed identification of vanA+ samples from which VRE could not be isolated when selective culture methods were used. From such a sample a vancomycin-susceptible, vanA+ Enterococcus faecium, Efm-V1511, was isolated, when vancomycin selection was not used during culture. Similar isolates with variable susceptibility to vancomycin were obtained in the following months., Objectives: To characterize Efm-V1511 and investigate the causes of variable susceptibility to vancomycin., Methods: All strains were sequenced using Illumina technology. Plasmids containing vanA were reconstructed by scaffolding to known plasmids or plasmids were sequenced using Oxford Nanopore MinION. Derived structures were verified by PCR and sequencing. Furthermore, selected vanA+ vancomycin-susceptible isolates were passaged in the presence of vancomycin and vancomycin-resistant variants obtained were sequenced., Results: Efm-V1511 belonged to ST1421 and contained a 49 696 bp plasmid pHVH-V1511 carrying a Tn1546-derived genetic element. Within this element vanX was truncated by a 252 bp 3' deletion explaining the susceptibility of Efm-V1511. Between March 2016 and April 2017, 48 isolates containing pHVH-V1511 were identified. All were ST1421. In isolates resistant to vancomycin, resistance could be attributed to changes in ddl disrupting gene function sometimes accompanied by changes in vanS, increased pHVH-V1511 copy number or the existence of an additional vanA-containing plasmid encoding a functional vanX., Conclusions: E. faecium carrying pHVH-V1511 is capable of nosocomial transmission and may develop clinical resistance to vancomycin. Strains may not be detected using standard culture methods for VRE.

Published
2018
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