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The SCK CEN Academy for Nuclear Science and Technology functions as the umbrella structure coordinating the education and training activities of the Belgian Nuclear Research Centre (SCK CEN). One of the main activities of the SCK CEN Academy is to provide customised training to professionals working in the nuclear industry, healthcare, research or governmental institutions. The courses and practical sessions are usually given in a face-to-face (FTF) format. This has drastically changed in the last 2 y because of the COVID-19 pandemic when it was necessary to switch course delivery from FTF to an online format. Feedback was collected from trainees and trainers in different training courses in radiation protection provided in FTF and online format. The analysis of this feedback allows training providers to make better-informed decisions on the best-suited training format depending on the content, target audience and the duration of the learning activity.

At the Belgian Nuclear Research Centre (SCK CEN) more than 850 employees advance the peaceful applications of ionising radiation through research, services and education and training. Several initiatives are taken to guarantee a safe working environment, one of them being a compulsory introductory safety training for all new employees, followed by refresher courses. With the objective to increase the flexibility for the participants and to optimise the teaching time of lecturers, the training format is adapted from face-to-face training to blended learning in which a significant amount of online learning is introduced. To appraise the perception and effectiveness of the original face-to-face training and the newly developed e-learning modules, and to compare both, information is gathered on two levels. Firstly, to monitor the perception of the participants regarding the face-to-face and e-learning formats an online questionnaire was launched. Secondly to evaluate the knowledge gain (difference between pre- and post-test results), the participants were requested to answer the same set of technical/scientific questions before and after the training. Results of the perception study show that the e-learning format was well received by the learners. For both learning formats an overall knowledge gain was noticed. The highest knowledge gain was noticed for the e-learning format. The results on perception and effectivity of the e-learning format confirm the further use of this format.

Objectives: This review aimed to present studies that prospectively investigated biological effects in patients following diagnostic dentomaxillofacial radiology (DMFR). Methods: Literature was systematically searched to retrieve all studies assessing radiobiological effects of using X-ray imaging in the dentomaxillofacial area, with reference to radiobiological outcomes for other imaging modalities and fields. Results: There is a lot of variability in the reported radiobiological assessment methods and radiation dose measures, making comparisons of radiobiological studies challenging. Most radiological DMFR studies are focusing on genotoxicity and cytotoxicity, data for 2D dentomaxillofacial radiographs, albeit with some methodological weakness biasing the results. For CBCT, available evidence is limited and few studies include comparative data on both adults and children. Conclusions In the future, one will have to strive towards patient-specific measures by considering age, gender and other individual radiation sensitivity-related factors. Ultimately, future radioprotection strategies should build further on the concept of personalized medicine, with patient-specific optimization of the imaging protocol, based on radiobiological variables.

The European Joint Programme on Radioactive Waste Management (EURAD – H2020, grant agreement No. 847593) aims to achieve a step-wise change in European collaboration towards safe radioactive waste management (RWM) through the development of a robust and sustained science, technology and knowledge management (KM) programme. EURAD has three KM work packages. The main goals are to (i) preserve generated knowledge, (ii) transfer knowledge to Member States with early-stage RWM programmes, (iii) transfer knowledge between generations, and (iv) disseminate knowledge. The EURAD work package (WP) on “Training & mobility” aids in achieving these goals through its “School of Radioactive Waste Management”. The School of RWM supports competence building in RWM matters. Currently four distinguished initiatives can be highlighted: the organization of training courses, the hosting of webinars, the coordination of a mobility programme, and actions to support the EURAD PhD community. The School of RWM has a dedicated webpage (http://www.euradschool.eu, last access: 30 September 2021) where all information on its activities can be found. A portfolio of basic and specialized training courses was set up containing an up-to-date list of existing training initiatives and new training courses. The latter are based on a gap analysis performed within the framework of the priorities set in the EURAD Roadmap and on the end-user needs. The School of RWM gives attention to the best-fitted training format and scientific state-of-the-art is guaranteed through the lecturing by EURAD and/or external subject experts. Besides training courses, webinars on specific topics that are of relevance to the entire EURAD community are hosted on a regular basis. These so-called Lunch & Learn sessions are short, informal online get-togethers, triggering discussion and debate. Presentations are given by experts from the EURAD community and beyond, such as from the International Atomic Energy Agency (IAEA) or the Organisation for Economic Co-operation and Development/Nuclear Energy Agency (OECD/NEA) and can deal with all topics that link to RWM, from very specialized scientific matters to more general and overarching themes. The School of RWM also coordinates a mobility programme, allowing its beneficiaries to perform technical visits to infrastructures from EURAD partners or end-users, to undertake internships and to set up exchange programmes between organizations within EURAD. These activities serve as enhanced training as well as collaboration between all members of the EURAD community. These mobility actions can be complementary to an educational or training programme and/or part of a continuous personal development programme. Last but not least, the initiative was taken to support the EURAD PhD community. As future key players in the field of RWM, they will benefit from the possibilities for early networking with their peers and with established RWM experts. With these four initiatives, the School of RWM, and by extension the “Training & Mobility” WP, is an integral part of EURAD's KM programme. This presentation describes how the School of RWM is linked to the EURAD Roadmap, what its goal and long-term objectives are and how it aids in achieving EURAD's KM goals.

Assessing the possible biological effects of exposure to low doses of ionizing radiation (IR) is one of the prime challenges in radiation protection, especially in medical imaging. Today, radiobiological data on cone beam CT (CBCT) related biological effects are scarce. In children and adults, the induction of DNA double strand breaks (DSBs) in buccal mucosa cells and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) and antioxidant capacity in saliva samples after CBCT examination were examined. No DNA DSBs induction was observed in children nor adults. In children only, an increase in 8-oxo-dG levels was observed 30 minutes after CBCT. At the same time an increase in antioxidant capacity was observed in children, whereas a decrease was observed in adults. Our data indicate that children and adults react differently to IR doses associated with CBCT. Fully understanding these differences could lead to an optimal use of CBCT in different age categories as well as improved radiation protection guidelines. ispartof: SCIENTIFIC REPORTS vol:10 issue:1 ispartof: location:England status: published

The use of carbon ion therapy for cancer treatment is becoming more widespread due to the advantages of carbon ions compared with X‑rays. Breast cancer patients may benefit from these advantages, as the surrounding healthy tissues receive a lower dose, and the increased biological effectiveness of carbon ions can better control radioresistant cancer cells. Accumulating evidence indicates that the Hedgehog (Hh) pathway is linked to the development and progression of breast cancer, as well as to resistance to X‑irradiation and the migratory capacity of cancer cells. Hence, there is an increasing interest in targeting the Hh pathway in combination with radiotherapy. Several studies have already investigated this treatment strategy with conventional radiotherapy. However, to the best of our knowledge, the combination of Hh inhibitors with particle therapy has not yet been explored. The aim of the present study was to investigate the potential of the Hh inhibitor GANT61 as an effective modulator of radiosensitivity and migration potential in MCF‑7 breast cancer cells, and compare potential differences between carbon ion irradiation and X‑ray exposure. Although Hh targeting was not able to radiosensitise cells to any radiation type used, the combination of GANT61 with X‑rays or carbon ions (energy: 95 MeV/n; linear energy transfer: 73 keV/µm) was more effective in decreasing MCF‑7 cell migration compared with either radiation type alone. Gene expression of the Hh pathway was affected to different degrees in response to X‑ray and carbon ion irradiation, as well as in response to the combination of GANT61 with irradiation. In conclusion, combining Hh inhibition with radiation (X‑rays or carbon ions) more effectively decreased breast cancer cell migration compared with radiation treatment alone. ispartof: INTERNATIONAL JOURNAL OF ONCOLOGY vol:55 issue:6 pages:1339-1348 ispartof: location:Greece status: published

Objectives: Cone-beam CT (CBCT) is a medical imaging technique used in dental medicine. However, there are no conclusive data available indicating that exposure to X-ray doses used by CBCT are harmless. We aim, for the first time, to characterize the potential age-dependent cellular and subcellular effects related to exposure to CBCT imaging. Current objective is to describe and validate the protocol for characterization of cellular and subcellular changes after diagnostic CBCT. Methods: Development and validation of a dedicated two-part protocol: 1) assessing DNA double strand breaks (DSBs) in buccal mucosal (BM) cells and 2) oxidative stress measurements in saliva samples. BM cells and saliva samples are collected prior to and 0.5 h after CBCT examination. BM cells are also collected 24 h after CBCT examination. DNA DSBs are monitored in BM cells via immunocytochemical staining for γH2AX and 53BP1. 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxo-dG) and total antioxidant capacity are measured in saliva to assess oxidative damage. Results: Validation experiments show that sufficient BM cells are collected (97.1 ± 1.4 %) and that γH2AX/53BP1 foci can be detected before and after CBCT examination. Collection and analysis of saliva samples, either sham exposed or exposed to IR, show that changes in 8-oxo-dG and total antioxidant capacity can be detected in saliva samples after CBCT examination. Conclusion: The DIMITRA Research Group presents a two-part protocol to analyze potential age-related biological differences following CBCT examinations. This protocol was validated for collecting BM cells and saliva and for analyzing these samples for DNA DSBs and oxidative stress markers, respectively.

Objectives: Cone-beam CT (CBCT), a radiographic tool for diagnosis, treatment, and follow-up in dental practice, was introduced also in pediatric radiology, especially orthodontics. Such patients subjected to repetitive X-rays examinations may receive substantial levels of radiation doses. Ionizing radiation (IR), a recognized carcinogenic factor causing DNA double-strand breaks (DSBs) could be harmful to undifferentiated cells such as dental pulp stem cells (DPSCs) since inaccurately repaired or unrepaired DSBs may lead to malignant transformation. The H2AX and MRE11 proteins generated following DSBs formation and pro-inflammatory cytokines (CKs) secreted after irradiation are relevant candidates to monitor the cellular responses induced by CBCT. Methods: DPSCs were extracted from human exfoliated deciduous teeth and their phenotype was assessed by immunocytochemistry and flow-cytometry. Cells were exposed to IR doses: 5.4-107.7 mGy, corresponding to 0.5-8 consecutive skull exposures, respectively. H2AX and MRE11 were detected in whole cells, while IL-1 alpha, IL-6, IL-8, TNF alpha in supernatants, using enzyme-linked immunosorbent assay (ELISA) at different time points after exposure. Results: The phosphorylation level of H2AX in DPSCs increased considerably at 0.5 h after exposure (p < 0.001 for 3, 5, 8 skull exposures and p < 0.05 for 1 skull exposure, respectively). MRE11 response could only be detected for the highest IR dose (p < 0.001) in the same interval. CKs secretion increased upon CBCT exposure according to doses and time. Conclusions: The DPSCs exposure to CBCT induces transient DNA damage and persistent inflammatory reaction in DPSCs drawing the attention on the potential risks of IR exposures and on the importance of dose monitoring in pediatric population. The research leading to these results has received funding from the European Atomic Energy Community's Seventh Framework Programme FP7/2007-2011 under grant agreement no 604984 (OPERRA: Open Project for the European Radiation Research Area).

Due to the advantages of charged particles compared to conventional radiotherapy, a vast increase is noted in the use of particle therapy in the clinic. These advantages include an improved dose deposition and increased biological effectiveness. Metastasis is still an important cause of mortality in cancer patients and evidence has shown that conventional radiotherapy can increase the formation of metastasizing cells. An important pathway involved in the process of metastasis is the Hedgehog (Hh) signaling pathway. Recent studies have demonstrated that activation of the Hh pathway, in response to X-rays, can lead to radioresistance and increased migratory, and invasive capabilities of cancer cells. Here, we investigated the effect of X-rays, protons, and carbon ions on cell survival, migration, and Hh pathway gene expression in prostate cancer (PC3) and medulloblastoma (DAOY) cell lines. In addition, the potential modulation of cell survival and migration by the Hh pathway inhibitor GANT61 was investigated. We found that in both cell lines, carbon ions were more effective in decreasing cell survival and migration as well as inducing more significant alterations in the Hh pathway genes compared to X-rays or protons. In addition, we show here for the first time that the Hh inhibitor GANT61 is able to sensitize DAOY medulloblastoma cells to particle radiation (proton and carbon ion) but not to conventional X-rays. This important finding demonstrates that the results of combination treatment strategies with X-ray radiotherapy cannot be automatically extrapolated to particle therapy and should be investigated separately. In conclusion, combining GANT61 with particle radiation could offer a benefit for specific cancer types with regard to cancer cell survival. KK is a recipient of a SCK.CEN-KUL PhD grant. KK, NB, MV, and BB received a Horizon2020 travel grant (No. 654002) for experiments at GANIL. KK received an EU CONCERT travel grant and Belgian Hadron therapy Consortium (BHTC) travel grant, whereas MM received an FWO travel grant to perform experiments at iThemba LABS in South-Africa. KH is a clinical research fellow of the Research Foundation Flanders, Belgium.

Knowledge management is a core activity for the European Member States (MSs) implementing the Council Directive EC 2011/70/EURATOM. Twenty-one MSs and two associated countries have mandated their respective radioactive waste management, safety and research organisations to contribute to the European Joint Programme on Radioactive Waste Management (EURAD). EURAD has established a Knowledge Management and Networking Programme which supports the capturing of knowledge and its transfer among organisations, Member States and generations. EURAD as a Joint Programming has an utmost advantage, compared to individual projects, as it provides:processes for knowledge sharing, for example interaction between the different radioactive waste management (RWM) actors to find out what is already known and what is most useful to investigate further.Resources and people to develop new knowledge and/or to support preservation of existing knowledge at risk, for example access to experts, networks and communities of practice.Tools and technology capable of handling different forms of knowledge, with a focus on socialising, signposting and aggregating existing knowledge sources.This paper intends to describe the role of knowledge management and networking in EURAD, how knowledge generated by EURAD Workpackages and RWM organisations is captured and how we provide added value to MSs. Furthermore, it explains how we cooperate and work together towards common knowledge preservation goals with the EC PREDIS project, IAEA and OECD/NEA, to avoid duplication of work and maximise impact.

Knowledge management (KM) is critical to ensure safe and sustainable radioactive waste management. The European Commission is encouraging KM through the Waste Directive. EURAD and PREDIS are projects funded by the European Commission, including work packages devoted to Knowledge Management. EURAD has set up a “School of RWM”, launched in 2020. To establish a portfolio of training courses, currently existing training initiatives in the field of RWM were mapped. A gap analysis was carried out to detect current unfulfilled needs for courses on specific topics. The School of RWM has already organized two training courses responding to the perceived needs. Besides training courses, both projects regularly organize topical webinars, which are related to RWM & Predisposal-related themes. Both projects launched a dedicated mobility program, allowing their beneficiaries to perform technical visits to infrastructures, undertake internships and set up exchange programs between institutions within EURAD/PREDIS. In addition, PREDIS also financially supports participation in training courses and events. Finally, both projects support their respective student communities, including masters, Ph.D. students, and postdocs. In order to improve their efficiency, EURAD and PREDIS KM will be merged in the proposed upcoming EURAD-2 program.