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1. Antigen 5–spiked Vespula and Polistes venom extracts for Vespid allergy diagnostics: A French multicenter study

Author

Claude Lambert, M. Drouet, Françoise Bienvenu, P.A. Apoil, Charles Dzviga, Thierry Tabary, D. Mariotte, Séverine Brabant, Laurence Guilloux, François Lavaud, Anne Sarrat, Joana Vitte, Pascale Nicaise-Roland, Joëlle Birnbaum, Céline Beauvillain, Nicolas Hutt, Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), Service de dermatologie, vénéreologie et cancérologie cutanée [Hôpital de la Timone - APHM], Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Nouvel Hôpital Civil de Strasbourg, Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Service d'immunologie [Centre Hospitalier Lyon Sud - HCL], Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM), Centre de Recherche en Cancérologie et Immunologie Nantes-Angers (CRCINA), Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes), Institut d'Immunologie [CHRU Lille], Pôle de Biologie Pathologie Génétique [CHU Lille], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Eurofins Biomnis, Laboratoire d'Immunologie [CHU Caen], Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Centre Hospitalier Universitaire de Reims (CHU Reims), Unité Fonctionnelles d′Immunologie [CHU Bichat], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service d'immunologie et d'immunogénétique [Bordeaux], Université Bordeaux Segalen - Bordeaux 2-CHU Bordeaux [Bordeaux], Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), Unité différenciation épidermique et auto-immunité rhumatoïde (UDEAR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Toulouse [Toulouse], Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), and Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA)

Subjects
Male, 0301 basic medicine, Allergy, MESH: Immunoglobulin E / blood, Wasps, Wasp Venoms, Venom, Vespula, 0302 clinical medicine, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Child, MESH: Aged, 80 and over Allergens / immunology, Immunology and Allergy, MESH: Animals, MESH: Hypersensitivity / immunology, Child, Aged, 80 and over, MESH: Aged, MESH: Immunoglobulin E / immunology, MESH: Middle Aged, biology, Middle Aged, MESH: Infant, MESH: Young Adult, Child, Preschool, Female, France, Polistes, MESH: Wasp Venoms / immunology, MESH: Wasps / immunology, Adult, Pulmonary and Respiratory Medicine, Adolescent, Immunology, Young Adult, 03 medical and health sciences, Antigen, Hypersensitivity, medicine, Animals, Humans, MESH: Hypersensitivity / blood, Aged, MESH: Adolescent, MESH: Hypersensitivity / diagnosis, MESH: Humans, business.industry, MESH: Child, Preschool, Infant, MESH: Adult, Polistes spp, Allergens, Immunoglobulin E, biology.organism_classification, medicine.disease, MESH: Male, MESH: France, 030104 developmental biology, 030228 respiratory system, Multicenter study, business, MESH: Female
Abstract

International audience; Vespula and Polistes spp venom extracts (VEs) for ImmunoCAP (Thermo Fisher Scientific, Uppsala, Sweden) platforms have beenspiked, that is, enriched, with group 5 allergens since 2012. Limited information was available from the manufacturer, but small cohortshave reported altered performance for in vitro diagnostics.1,2 Herein we present the results of a French multicenter study onantigen 5–spiked Vespula and Polistes VEs compared with nonspiked VEs

Published
2018
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2. IgE-mediated anaphylaxis caused by bites of the pigeon tick Argas reflexus: cloning and expression of the major allergen Arg r 1

Author

Gabrielle Pauli, Christiane Hilger, J.C. Bessot, Fabienne Grigioni, François Hentges, Nicolas Hutt, and Frédéric de Blay

Subjects
Immunology, Immunoblotting, Molecular Sequence Data, Argas, Sequence Homology, Enzyme-Linked Immunosorbent Assay, Tick, medicine.disease_cause, Immunoglobulin E, law.invention, Microbiology, Allergen, law, Food allergy, Complementary DNA, parasitic diseases, medicine, Immunology and Allergy, Animals, Humans, Amino Acid Sequence, Bites and Stings, Cloning, Molecular, Anaphylaxis, Gene Library, biology, Argasidae, Allergens, biology.organism_classification, medicine.disease, Recombinant Proteins, Recombinant DNA, biology.protein, Electrophoresis, Polyacrylamide Gel
Abstract

Background Anaphylactic reactions caused by bites of the European pigeon tick Argas reflexus are repeatedly reported. This soft-backed tick is a parasite of wild pigeons colonizing urban buildings and houses. Occasionally the ticks can bite human beings, inducing anaphylactic reactions in sensitized patients. Objective Our aim was to characterize the major allergen implicated in a series of anaphylactic reactions caused by Argas bites and to produce the allergen as recombinant protein for diagnostic purposes. Methods Protein extracts were prepared from whole A reflexus bodies, and IgE immunoblots were performed with sera from 13 patients who had an anaphylactic reaction with pigeon tick bites. A cDNA expression library was constructed from whole ticks and screened with a polyclonal rabbit antiserum raised against the major allergen. Results The cDNA coding for the dominant allergen Arg r 1 could be isolated. It encodes a protein belonging to the lipocalin family. Allergenicity of the recombinant Arg r 1 was confirmed by immunoblot, ELISA, and intradermal skin tests. Conclusion The dominant allergen of A reflexus has been isolated and the corresponding cDNA cloned. The recombinant protein, a lipocalin, was expressed in Escherichia coli and was shown to be immunoreactive in vitro and in vivo. Recombinant Arg r 1 was used as a diagnostic tool in a series of anaphylactic reactions caused by pigeon tick bites.

Published
2005

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