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Abstract Background Abiotic stresses in plants include all the environmental conditions that significantly reduce yields, like drought and heat. One of the most significant effects they exert at the cellular level is the accumulation of reactive oxygen species, which cause extensive damage. Plants possess two mechanisms to counter these molecules, i.e. detoxifying enzymes and non-enzymatic antioxidants, which include many classes of specialized metabolites. Sunflower, the fourth global oilseed, is considered moderately drought resistant. Abiotic stress tolerance in this crop has been studied using many approaches, but the control of specialized metabolites in this context remains poorly understood. Here, we performed the first genome-wide association study using abiotic stress-related specialized metabolites as molecular phenotypes in sunflower. After analyzing leaf specialized metabolites of 450 hybrids using liquid chromatography-mass spectrometry, we selected a subset of these compounds based on their association with previously known abiotic stress-related quantitative trait loci. Eventually, we characterized these molecules and their associated genes. Results We putatively annotated 30 compounds which co-localized with abiotic stress-related quantitative trait loci and which were associated to seven most likely candidate genes. A large proportion of these compounds were potential antioxidants, which was in agreement with the role of specialized metabolites in abiotic stresses. The seven associated most likely candidate genes, instead, mainly belonged to cytochromes P450 and glycosyltransferases, two large superfamilies which catalyze greatly diverse reactions and create a wide variety of chemical modifications. This was consistent with the high plasticity of specialized metabolism in plants. Conclusions This is the first characterization of the genetic control of abiotic stress-related specialized metabolites in sunflower. By providing hints concerning the importance of antioxidant molecules in this biological context, and by highlighting some of the potential molecular mechanisms underlying their biosynthesis, it could pave the way for novel applications in breeding. Although further analyses will be required to better understand this topic, studying how antioxidants contribute to the tolerance to abiotic stresses in sunflower appears as a promising area of research.

The Study Demands and Resources Scale (SDRS) has shown promise as a valid and reliable measure for measuring students’ specific study demands and -resources. However, there is no evidence as to its psychometric properties outside of the original context in which it was developed. This study aimed to assess the psychometric properties of the SDRS in a cross-national student population through examining its longitudinal factorial validity, internal consistency, and temporal invariance as well as criterion validity through its association with study engagement and task performance over time. Results showed that a Bifactor Exploratory Structural Equation Model (ESEM) with one general factor (overall study characteristics) and five specific factors (workload, growth opportunities, lecturer support, peer support, information availability) fitted the data, showed strong measurement invariance over time, and was reliable at different time points. The study further established criterion validity for the overall study characteristics factor through its concurrent and predictive associations with study engagement and task performance. However, the specific factors’ concurrent and predictive capacity could only partially be established when controlling for the general study characteristics factor. These findings suggest that study characteristics should be measured as a dynamic interaction between study demands and resources, rather than a hierarchical model.

Academic task performance (TP) refers to the proficiency with which students perform in academic tasks through making the right choices and completing core tasks central to their academic studies, on time and to specification. We adapted Koopmans et al.’s task performance scale (TPS) for use within tertiary education and investigated its psychometric properties, internal consistency and measurement invariance across age, gender and national groups in university students (n = 3,265). The results showed that a hierarchical ESEM model with one higher-order task performance factor consisting of time management and task efficiency fitted the data best. The TPS exhibited full measurement invariance across gender and age groups, implying that the latent mean scores can be used to determine differences. However, invariance could only partially be established for national cohorts, implying that cross-national comparisons may not be possible. These findings offer preliminary support for the TPS as a valid instrument for gauging students’ academic task performance.

Abstract BRAF mutations occur early in serrated colorectal cancers, but their long-term influence on tissue homeostasis is poorly characterized. We investigated the impact of short-term (3 days) and long-term (6 months) expression of Braf V600E in the intestinal tissue of an inducible mouse model. We show that Braf V600E perturbs the homeostasis of intestinal epithelial cells, with impaired differentiation of enterocytes emerging after prolonged expression of the oncogene. Moreover, Braf V600E leads to a persistent transcriptional reprogramming with enrichment of numerous gene signatures indicative of proliferation and tumorigenesis, and signatures suggestive of metabolic rewiring. We focused on the top-ranking cholesterol biosynthesis signature and confirmed its increased expression in human serrated lesions. Functionally, the cholesterol lowering drug atorvastatin prevents the establishment of intestinal crypt hyperplasia in Braf V600E -mutant mice. Overall, our work unveils the long-term impact of Braf V600E expression in intestinal tissue and suggests that colorectal cancers with mutations in BRAF might be prevented by statins.

Abstract Background The prevalence of obesity and comorbidities is high in the population with spinal cord injury (SCI). We sought to determine the effect of SCI on the functional form of the relationship between body mass index (BMI) and risk of developing nonalcoholic fatty liver disease (NAFLD), and assess whether SCI‐specific mapping of BMI to risk of developing NAFLD is needed. Methods Longitudinal cohort study comparing Veterans Health Administration patients with a diagnosis of SCI to a 1:2 matched control group without SCI. The relationship between BMI and development of NAFLD at any time was assessed with propensity score matched Cox regression models; NAFLD development at 10‐year with a propensity score matched logistic model. The positive predictive value of developing NAFLD at 10 years was calculated for BMI 19–45 kg/m2. Results 14,890 individuals with SCI met study inclusion criteria, and 29,780 Non‐SCI individuals in matched control group. Overall, 9.2% in SCI group and 7.3% in Non‐SCI group developed NAFLD during the study period. A logistic model assessing the relationship between BMI and the probability of developing a diagnosis of NAFLD demonstrated that the probability of developing disease increased as BMI increased in both cohorts. The probability was significantly higher in the SCI cohort at each BMI threshold (p

We observed the ultrahot Jupiter WASP-33b with the SpectroPolarimètre InfraRouge on the Canada–France–Hawaii Telescope. Previous observations of the dayside of WASP-33b show evidence of CO and Fe emission indicative of a thermal inversion. We observed its nightside over five Earth nights to search for spectral signatures of CO in the planet’s thermal emission. Our three pretransit observations and two posttransit observations are sensitive to regions near the morning or evening terminators, respectively. From spectral retrievals, we detect CO molecular absorption in the planet’s emission spectrum after transit at ∼6.6 σ . This is the strongest ground-based detection of nightside thermal emission from an exoplanet and only the third ever. CO appearing in absorption suggests that the nightside near the evening terminator does not have a temperature inversion; this makes sense if the dayside inversion is driven by absorption of stellar radiation. On the contrary, we do not detect CO from the morning terminator. This may be consistent with heat advection by an eastward jet. Phase-resolved high-resolution spectroscopy offers an economical alternative to space-based full-orbit spectroscopic phase curves for studying the vertical and horizontal atmospheric temperature profiles of short-period exoplanets.

LHS 1140 b is the second-closest temperate transiting planet to Earth with an equilibrium temperature low enough to support surface liquid water. At 1.730 ± 0.025 R _⊕ , LHS 1140 b falls within the radius valley separating H _2 -rich mini-Neptunes from rocky super-Earths. Recent mass and radius revisions indicate a bulk density significantly lower than expected for an Earth-like rocky interior, suggesting that LHS 1140 b could be either a mini-Neptune with a small envelope of hydrogen (∼0.1% by mass) or a water world (9%–19% water by mass). Atmospheric characterization through transmission spectroscopy can readily discern between these two scenarios. Here we present two JWST/NIRISS transit observations of LHS 1140 b, one of which captures a serendipitous transit of LHS 1140 c. The combined transmission spectrum of LHS 1140 b shows a telltale spectral signature of unocculted faculae (5.8 σ ), covering ∼20% of the visible stellar surface. Besides faculae, our spectral retrieval analysis reveals tentative evidence of residual spectral features, best fit by Rayleigh scattering from a N _2 -dominated atmosphere (2.3 σ ), irrespective of the consideration of atmospheric hazes. We also show through Global Climate Models (GCMs) that H _2 -rich atmospheres of various compositions (100×, 300×, 1000× solar metallicity) are ruled out to >10 σ . The GCM calculations predict that water clouds form below the transit photosphere, limiting their impact on transmission data. Our observations suggest that LHS 1140 b is either airless or, more likely, surrounded by an atmosphere with a high mean molecular weight. Our tentative evidence of a N _2 -rich atmosphere provides strong motivation for future transmission spectroscopy observations of LHS 1140 b.

The hot Neptune desert is one of the most sparsely populated regions of the exoplanet parameter space, and atmosphere observations of its few residents can provide insights into how such planets have managed to survive in such an inhospitable environment. Here, we present transmission observations of LTT 9779 b, the only known hot Neptune to have retained a significant H/He-dominated atmosphere, taken with JWST NIRISS/SOSS. The 0.6–2.85 μ m transmission spectrum shows evidence for muted spectral features, rejecting a perfectly flat line at >5 σ . We explore water- and methane-dominated atmosphere scenarios for LTT 9779 b’s terminator, and retrieval analyses reveal a continuum of potential combinations of metallicity and cloudiness. Through comparisons to previous population synthesis works and our own interior structure modeling, we are able to constrain LTT 9779 b’s atmosphere metallicity to 20–850× solar. Within this range of metallicity, our retrieval analyses prefer solutions with clouds at millibar pressures, regardless of whether the atmosphere is water or methane dominated—though cloud-free atmospheres with metallicities >500× solar cannot be entirely ruled out. By comparing self-consistent atmosphere temperature profiles with cloud condensation curves, we find that silicate clouds can readily condense in the terminator region of LTT 9779 b. Advection of these clouds onto the dayside could explain the high dayside albedo previously inferred for this planet and be part of a feedback loop aiding the survival of LTT 9779 b’s atmosphere in the hot Neptune desert.

The two-planet transiting system LHS 1140 has been extensively observed since its discovery in 2017, notably with Spitzer, HST, TESS, and ESPRESSO, placing strong constraints on the parameters of the M4.5 host star and its small temperate exoplanets, LHS 1140 b and c. Here, we reanalyze the ESPRESSO observations of LHS 1140 with the novel line-by-line framework designed to fully exploit the radial velocity content of a stellar spectrum while being resilient to outlier measurements. The improved radial velocities, combined with updated stellar parameters, consolidate our knowledge of the mass of LHS 1140 b (5.60 ± 0.19 M _⊕ ) and LHS 1140 c (1.91 ± 0.06 M _⊕ ) with an unprecedented precision of 3%. Transits from Spitzer, HST, and TESS are jointly analyzed for the first time, allowing us to refine the planetary radii of b (1.730 ± 0.025 R _⊕ ) and c (1.272 ± 0.026 R _⊕ ). Stellar abundance measurements of refractory elements (Fe, Mg, and Si) obtained with NIRPS are used to constrain the internal structure of LHS 1140 b. This planet is unlikely to be a rocky super-Earth, as previously reported, but rather a mini-Neptune with a ∼0.1% H/He envelope by mass or a water world with a water-mass fraction between 9% and 19%, depending on the atmospheric composition and relative abundance of Fe and Mg. While the mini-Neptune case would not be habitable, a water-abundant LHS 1140 b potentially has habitable surface conditions according to 3D global climate models, suggesting liquid water at the substellar point for atmospheres with relatively low CO _2 concentration, from Earth-like to a few bars.

Color TSFG microscopy is a novel approach for label-free imaging of red blood cells and oxygenation in situ.

Collective cell migration is an important process during biological development and tissue repair but may turn malignant during tumor invasion. Mathematical and computational models are essential to unravel the mechanisms of self-organization that underlie the emergence of collective migration from the interactions among individual cells. Recently, guidance-by-followers was identified as one such underlying mechanism of collective cell migration in the embryo of the zebrafish. This poses the question of how the guidance stimuli are integrated when multiple cells interact simultaneously. In this study, we extend a recent individual-based model by an integration step of the vectorial guidance stimuli and compare model predictions obtained for different variants of the mechanism (arithmetic mean of stimuli, dominance of stimulus with largest transmission interface, and dominance of most head-on stimulus). Simulations are carried out and quantified within the modeling and simulation framework Morpheus. Collective cell migration is found to be robust and qualitatively identical for all considered variants of stimulus integration. Moreover, this study highlights the role of individual-based modeling approaches for understanding collective phenomena at the population scale that emerge from cell-cell interactions.

Super-Earths orbiting M dwarf stars may be the most common habitable planets in the Universe. However, their habitability is threatened by intense irradiation from their host stars, which drives the escape of water to space and can lead to surface desiccation. We present simulation results of a box model incorporating deep-water cycling between interior and atmosphere and water loss to space for terrestrial planets of mass 1–8 M _⊕ orbiting in the habitable zone of a late M dwarf. Energy-limited loss decreases with planetary mass, while diffusion-limited loss increases with mass. Depending on where it orbits in the habitable zone, a 1 M _⊕ planet that starts with 3–8 Earth Oceans can end up with an Earthlike surface of oceans and exposed continents; for an 8 M _⊕ super-Earth, that range is 3–12 Earth Oceans. Planets initialized with more water end up as waterworlds with no exposed continents, while planets that start with less water have desiccated surfaces by 5 Gyr. Since the mantles of terrestrial planets can hold much more water than is currently present in Earth’s atmosphere, none of our simulations result in Dune planets—such planets may be less common than previously thought. Further, more water becomes sequestered within the mantle for larger planets. A super-Earth at the inner edge of the habitable zone tends to end up as either a waterworld or with a desiccated surface; only a narrow range of initial water inventory yields an Earthlike surface.

Searches for helium in the exospheres of exoplanets via the metastable near-infrared triplet have yielded 17 detections and 40 nondetections. We performed a comprehensive reanalysis of published studies to investigate the influence of stellar X-ray and extreme-ultraviolet (XUV) flux and orbital parameters on the detectability of helium in exoplanetary atmospheres. We identified a distinct “orbital sweet spot” for helium detection, 0.03 to 0.08 au from the host star, where the majority of detections occurred. This sweet spot is influenced by the stellar luminosity and planet size. Notably, a lower ratio of XUV flux to mid-UV flux is preferred for planets compared to nondetections. We also found that helium detections occur for planets around stars with effective temperatures of 4400–6500 K (i.e., spectral type K and G stars), with a sharp gap between 5400 and 6000 K, where no detections occur. We also report an upper-limit efficiency of 6% for energy-limited atmospheric escape from our analysis. Additionally, our analysis of the cumulative XUV flux versus escape velocity shows planets with helium detections above the “cosmic shoreline,” where atmospheres are not thought to be present, suggesting the shoreline needs revision. The unexpected trends revealed in our meta-analysis can contribute to a better understanding of star–planet interaction and exosphere evolution.

JWST has ushered in an era of unprecedented ability to characterize exoplanetary atmospheres. While there are over 5000 confirmed planets, more than 4000 Transiting Exoplanet Survey Satellite (TESS) planet candidates are still unconfirmed and many of the best planets for atmospheric characterization may remain to be identified. We present a sample of TESS planets and planet candidates that we identify as “best-in-class” for transmission and emission spectroscopy with JWST. These targets are sorted into bins across equilibrium temperature T _eq and planetary radius R _p and are ranked by a transmission and an emission spectroscopy metric (TSM and ESM, respectively) within each bin. We perform cuts for expected signal size and stellar brightness to remove suboptimal targets for JWST. Of the 194 targets in the resulting sample, 103 are unconfirmed TESS planet candidates, also known as TESS Objects of Interest (TOIs). We perform vetting and statistical validation analyses on these 103 targets to determine which are likely planets and which are likely false positives, incorporating ground-based follow-up from the TESS Follow-up Observation Program to aid the vetting and validation process. We statistically validate 18 TOIs, marginally validate 31 TOIs to varying levels of confidence, deem 29 TOIs likely false positives, and leave the dispositions for four TOIs as inconclusive. Twenty-one of the 103 TOIs were confirmed independently over the course of our analysis. We intend for this work to serve as a community resource and motivate formal confirmation and mass measurements of each validated planet. We encourage more detailed analysis of individual targets by the community.

Short-period exoplanets on circular orbits are thought to be tidally locked into synchronous rotation. If tidally locked, these planets must possess permanent day- and night-sides, with extreme irradiation on the dayside and none on the nightside. However, so far the tidal locking hypothesis for exoplanets is supported by little to no empirical evidence. Previous work showed that the super-Earth LHS 3844b likely has no atmosphere, which makes it ideal for constraining the planet’s rotation. Here we revisit the Spitzer phase curve of LHS 3844b with a thermal model of an atmosphere-less planet and analyze the impact of nonsynchronous rotation, eccentricity, tidal dissipation, and surface composition. Based on the lack of observed strong tidal heating we rule out rapid nonsynchronous rotation (including a Mercury-like 3:2 spin–orbit resonance) and constrain the planet's eccentricity to less than ∼0.001 (more circular than Io's orbit). In addition, LHS 3844b’s phase curve implies that the planet either still experiences weak tidal heating via a small-but-nonzero eccentricity (requiring an undetected orbital companion), or that its surface has been darkened by space weathering; of these two scenarios we consider space weathering more likely. Our results thus support the hypothesis that short-period rocky exoplanets are tidally locked, and further show that space weathering can significantly modify the surfaces of atmosphere-less exoplanets.

TRAPPIST-1 is a nearby system of seven Earth-sized, temperate, rocky exoplanets transiting a Jupiter-sized M8.5V star, ideally suited for in-depth atmospheric studies. Each TRAPPIST-1 planet has been observed in transmission both from space and from the ground, confidently rejecting cloud-free, hydrogen-rich atmospheres. Secondary eclipse observations of TRAPPIST-1 b with JWST/MIRI are consistent with little to no atmosphere given the lack of heat redistribution. Here we present the first transmission spectra of TRAPPIST-1 b obtained with JWST/NIRISS over two visits. The two transmission spectra show moderate to strong evidence of contamination from unocculted stellar heterogeneities, which dominates the signal in both visits. The transmission spectrum of the first visit is consistent with unocculted starspots and the second visit exhibits signatures of unocculted faculae. Fitting the stellar contamination and planetary atmosphere either sequentially or simultaneously, we confirm the absence of cloud-free, hydrogen-rich atmospheres, but cannot assess the presence of secondary atmospheres. We find that the uncertainties associated with the lack of stellar model fidelity are one order of magnitude above the observation precision of 89 ppm (combining the two visits). Without affecting the conclusion regarding the atmosphere of TRAPPIST-1 b, this highlights an important caveat for future explorations, which calls for additional observations to characterize stellar heterogeneities empirically and/or theoretical works to improve model fidelity for such cool stars. This need is all the more justified as stellar contamination can affect the search for atmospheres around the outer, cooler TRAPPIST-1 planets for which transmission spectroscopy is currently the most efficient technique.

Abstract Background Leaf senescence delay impacts positively in grain yield by maintaining the photosynthetic area during the reproductive stage and during grain filling. Therefore a comprehensive understanding of the gene families associated with leaf senescence is essential. NAC transcription factors (TF) form a large plant-specific gene family involved in regulating development, senescence, and responses to biotic and abiotic stresses. The main goal of this work was to identify sunflower NAC TF (HaNAC) and their association with senescence, studying their orthologous to understand possible functional relationships between genes of different species. Results To clarify the orthologous relationships, we used an in-depth comparative study of four divergent taxa, in dicots and monocots, with completely sequenced genomes (Arabidopsis thaliana, Vitis vinifera, Musa acuminata and Oryza sativa). These orthologous groups provide a curated resource for large scale protein sequence annotation of NAC TF. From the 151 HaNAC genes detected in the latest version of the sunflower genome, 50 genes were associated with senescence traits. These genes showed significant differential expression in two contrasting lines according to an RNAseq assay. An assessment of overexpressing the Arabidopsis line for HaNAC001 (a gene of the same orthologous group of Arabidopsis thaliana ORE1) revealed that this line displayed a significantly higher number of senescent leaves and a pronounced change in development rate. Conclusions This finding suggests HaNAC001 as an interesting candidate to explore the molecular regulation of senescence in sunflower.

This investigation explored the feasibility of producing strong, good quality, durable concrete in low-clinker systems (less than 50% clinker in some cases). The low-clinker content was achieved by combining interground portland limestone cement (PLC) with high limestone contents and different supplementary cementitious materials (SCMs). Seven cements, with approximate limestone contents between 3% and 31%, from two cement plants were used, in combination with SCMs, in forty-two different mixtures with water-cementitious materials ratios (w/cm) of 0.40 and 0.45. The SCMs included Class F and C fly ashes, Grade 100 slag, and silica fume. Mechanical properties (compressive strength, tensile strength, elastic modulus) and electrical resistivity were measured at 1, 7, 28, and 91 days. Similar compressive strengths were observed for mixtures with equivalent effective w/cm ratios. Although, the combination of PLCs with SCMs for very low-clinker systems resulted in decreased compressive strength, an increase in electrical resistivity was observed. More importantly, strong, good-quality concrete can be produced without sacrificing environmental benefits.

Abstract Background The strength and size of core muscles, including the abdominal muscles, are crucial to proper function in most activities. Therefore, it is important to reliably assess these characteristics. Our primary objective was to determine if the length, thickness and cross-sectional area of the transversus abdominis (TrA) can be visualized independently from the internal and external abdominal oblique muscles using extended field of view ultrasound imaging at rest and with contraction and to establish its intra- and inter-tester reliability. Methods Twenty-six individuals were recruited to participate in the study (20 F, 6 M), average age 24.0 years (SD 9.4), height 170.7 cm (SD 8.6) and weight 63.9 kg (SD 9.0). From this total number of participants, two groups of 16 randomly selected participants were assessed to determine intra- and inter-tester reliability respectively. Extended field of view ultrasound images were obtained at three vertebral levels during rest and contraction in the side lying position for both the right and left sides of the trunk. Results Excellent intra-tester and inter-tester reliability was seen (ICC range of 0.972 to 0.984). The overall average percent standard error of the measurement for all measurements and locations was approximately 4%. The overall average minimal difference for the thickness measurement for the resting and contraction conditions combined were as follows: intratester 0.056 (0.014) cm and intertester 0.054 (0.017) cm, for area intratester 0.287 (0.086) cm2 and intertester 0.289 (0.101) cm2 and for length intratester 0.519 (0.097) cm and intertester 0.507 (0.085) cm. Conclusions Extended field of view ultrasound imaging is an effective method of reliably capturing clear images of the TrA during rest and contraction. It provides an efficient mechanism for the analysis of muscle morphology by being able to measure the cross-sectional area, thickness, and length on one image. This methodology is recommended for studies investigating TrA function and training.

Cultivated sunflower (Helianthus annuus L.) exhibits numerous phenotypic and transcriptomic responses to drought. However, the ways in which these responses vary with differences in drought timing and severity are insufficiently understood. We used phenotypic and transcriptomic data to evaluate the response of sunflower to drought scenarios of different timing and severity in a common garden experiment. Using a semi-automated outdoor high-throughput phenotyping platform, we grew six oilseed sunflower lines under control and drought conditions. Our results reveal that similar transcriptomic responses can have disparate phenotypic effects when triggered at different developmental time points. Leaf transcriptomic responses, however, share similarities despite timing and severity differences (e.g., 523 differentially expressed genes (DEGs) were shared across all treatments), though increased severity elicited greater differences in expression, particularly during vegetative growth. Across treatments, DEGs were highly enriched for genes related to photosynthesis and plastid maintenance. A co-expression analysis identified a single module (M8) enriched in all drought stress treatments. Genes related to drought, temperature, proline biosynthesis, and other stress responses were overrepresented in this module. In contrast to transcriptomic responses, phenotypic responses were largely divergent between early and late drought. Early-stressed sunflowers responded to drought with reduced overall growth, but became highly water-acquisitive during recovery irrigation, resulting in overcompensation (higher aboveground biomass and leaf area) and a greater overall shift in phenotypic correlations, whereas late-stressed sunflowers were smaller and more water use-efficient. Taken together, these results suggest that drought stress at an earlier growth stage elicits a change in development that enables greater uptake and transpiration of water during recovery, resulting in higher growth rates despite similar initial transcriptomic responses.

The production of bio-based composites with enhanced characteristics constitutes a strategic action to minimize the use of fossil fuel resources. The mechanical performances of these materials are related to the specific properties of their components, as well as to the quality of the interface between the matrix and the fibers. In a previous research study, it was shown that the polarity of the matrix played a key role in the mechanisms of fiber breakage during processing, as well as on the final properties of the composite. However, some key questions remained unanswered, and new investigations were necessary to improve the knowledge of the interactions between a lignocellulosic material and a polar matrix. In this work, for the first time, atomic force microscopy based on force spectroscopy measurements was carried out using functionalized tips to characterize the intermolecular interactions at the single molecule level, taking place between poly(butylene succinate) and four different plant fibers. The efficiency of the tip functionalization was checked out by scanning electron microscopy and energy-dispersive X-ray spectroscopy, whereas the fibers chemistry was characterized by Fourier-transform infrared spectroscopy. Larger interactions at the nanoscale level were found between the matrix and hypolignified fibers compared to lignified ones, as in control experiments on single lignocellulosic polymer films. These results could significantly aid in the design of the most appropriate composite composition depending on its final use.

Introduction: Individuals with spinal cord injury (SCI) are vulnerable to obesity. Annual obesity screening using body mass index (BMI) is standard of care mandated by U.S. Veterans Health Administration (VHA) guidelines. Our objective is to determine the rates, variability, and predictors of guideline-concordant annual screening for obesity, given potential challenges of height and weight measurements in individuals with SCI. Methods: This is a cross-sectional retrospective study using U.S. national VA databases. We identified all VHA patients with chronic SCI in fiscal year (FY) 2019, their treating facility, and frequency of recorded height and weight. We applied mixed-effects logistic regression models to assess associations between annual obesity screening and patient- and facility-level characteristics. Results: Of 20,978 individuals with chronic SCI in VHA in FY19, guideline-concordant annual BMI-screening was lacking in 37.9%. Accounting for facility level factors (geographic region, SCI facility type, volume of patients with SCI treated at the facility) a mixed-effects logistic regression model demonstrated that lack of annual obesity screening was significantly associated with older patient age (p

Abstract Background Stroke is a leading cause of disability and the fifth leading cause of death in the USA. Intravenous alteplase is a highly effective clot-dissolving stroke treatment that must be given in a hospital setting within a time-sensitive window. To increase the use of intravenous alteplase in stroke patients, many US counties enacted policies mandating emergency medical service (EMS) paramedics to bypass local emergency departments and instead directly transport patients to specially equipped stroke centers. The objective of this mixed-methods study is to evaluate the effectiveness of policy enactment as an implementation strategy, how differences in policy structures and processes impact effectiveness, and to explore how the county, hospital, and policy factors explain variation in implementation and clinical outcomes. This paper provides a detailed description of an Agency for Healthcare Quality and Research (AHRQ)-funded protocol, including the use of the Consolidated Framework for Implementation Research (CFIR) in the qualitative design. Methods/design We will construct the largest-ever national stroke database of Medicare enrollees (~ 1.5 million stroke patients) representing 896 policy counties paired with 1792 non-policy counties, then integrate patient-, hospital-, county-, and state-level covariates from eight different data sources. We will use a difference-in-differences analysis to estimate the overall effect of the policy enactment on intravenous alteplase use (implementation outcome) as well as key patient outcomes. We will also quantitatively examine if variation in the context (urban/rural status) and variation in policy features affect outcomes. Finally, a CFIR-informed multiple case study design will be used to interview informants in 72 stakeholders in 24 counties to identify and validate factors that enable policy effects. Discussion Policies can be potent implementation strategies. However, the effects of EMS bypass policies to increase intravenous alteplase use have not been rigorously evaluated. By learning how context and policy structures impact alteplase implementation, as well as the barriers and facilitators experienced by stakeholders responsible for policy enactment, the results of this study will inform decisions regarding if and how EMS bypass policies should spread to non-policy counties, and if indicated, creation of a “best practices” toolkit.

We present the first analysis of JWST near-infrared spectroscopy of stellar flares from TRAPPIST-1 during transits of rocky exoplanets. Four flares were observed from 0.6–2.8 μ m with the Near Infrared Imager and Slitless Spectrograph and 0.6–3.5 μ m with the Near Infrared Spectrograph during transits of TRAPPIST-1b, f, and g. We discover P α and Br β line emission and characterize flare continuum at wavelengths from 1–3.5 μ m for the first time. Observed lines include H α , P α –P ϵ , Br β , He i λ 0.7062 μ m, two Ca ii infrared triplet (IRT) lines, and the He i IRT. We observe a reversed Paschen decrement from P α –P γ alongside changes in the light-curve shapes of these lines. The continuum of all four flares is well described by blackbody emission with an effective temperature below 5300 K, lower than the temperatures typically observed at optical wavelengths. The 0.6–1 μ m spectra were convolved with the Transiting Exoplanet Survey Satellite (TESS) response, enabling us to measure the flare rate of TRAPPIST-1 in the TESS bandpass. We find flares of 10 ^30 erg, large enough to impact transit spectra occur at a rate of ${3.6}_{-1.3}^{+2.1}$ flare day ^−1 , ∼10× higher than previous predictions from K2. We measure the amount of flare contamination at 2 μ m for the TRAPPIST-1b and f transits to be 500 ± 450 and 2100 ± 400 ppm, respectively. We find up to 80% of flare contamination can be removed, with mitigation most effective from 1.0–2.4 μ m. These results suggest transits affected by flares may still be useful for atmospheric characterization efforts.

With the JWST we can now characterize the atmospheres of longer-orbit planets, but this moves us into a regime where we cannot assume that tidal forces from the star have eroded the planets’ obliquities and synchronized their rotation rates. These rotation vectors may be tracers of formation and evolution histories and also enable a range of atmospheric circulation states. Here we delineate the orbital space over which tidal synchronization and alignment assumptions may no longer apply and present three-dimensional atmospheric models of a hypothetical warm Jupiter over a range of rotation rates and obliquities. We simulate the secondary eclipses of this planet for different possible viewing orientations and times during its orbital, seasonal cycle. We find that the eclipse depth can be strongly influenced by the rotation rate and obliquity through the timing of the eclipse relative to the planet’s seasonal cycle, and advise caution in attempting to derive properties such as albedo or day–night transport from this measurement. We predict that if warm Jupiters beyond the tidal limit have intrinsic diversity in their rotation vectors, then this will manifest itself as dispersion in their secondary eclipse depths. We explore eclipse mapping as a way to uniquely constrain the rotation vector of warm Jupiters but find that the associated signals are likely at the edge of the JWST performance. Nevertheless, as the JWST begins to measure the secondary eclipses of longer-orbital-period planets, we should expect to observe the consequences of a wider range of rotation states and circulation patterns.

We present high-resolution dayside thermal emission observations of the exoplanet WASP-18 b using IGRINS on Gemini South. We remove stellar and telluric signatures using standard algorithms, and we extract the planet signal via cross-correlation with model spectra. We detect the atmosphere of WASP-18 b at a signal-to-noise ratio (S/N) of 5.9 using a full chemistry model, measure H _2 O (S/N = 3.3), CO (S/N = 4.0), and OH (S/N = 4.8) individually, and confirm previous claims of a thermal inversion layer. The three species are confidently detected (>4 σ ) with a Bayesian inference framework, which we also use to retrieve abundance, temperature, and velocity information. For this ultrahot Jupiter (UHJ), thermal dissociation processes likely play an important role. Retrieving abundances constant with altitude and allowing the temperature–pressure profile to adjust freely results in a moderately super-stellar carbon-to-oxygen ratio (C/O = ${0.75}_{-0.17}^{+0.14}$ ) and metallicity ([M/H] = ${1.03}_{-1.01}^{+0.65}$ ). Accounting for undetectable oxygen produced by thermal dissociation leads to C/O = ${0.45}_{-0.10}^{+0.08}$ and [M/H] = ${1.17}_{-1.01}^{+0.66}$ . A retrieval that assumes radiative–convective–thermochemical equilibrium and naturally accounts for thermal dissociation constrains C/O < 0.34 (2 σ ) and [M/H] = ${0.48}_{-0.29}^{+0.33}$ , in line with the chemistry of the parent star. Looking at the velocity information, we see a tantalizing signature of different Doppler shifts at the level of a few kilometers per second for different molecules, which might probe dynamics as a function of altitude and/or location on the planet disk. Our results demonstrate that ground-based, high-resolution spectroscopy at infrared wavelengths can provide meaningful constraints on the compositions and climate of highly irradiated planets. This work also elucidates potential pitfalls with commonly employed retrieval assumptions when applied to the spectra of UHJs.

The overarching goal of the present work is to contribute to the understanding of the relations between fetal heart rate (FHR) temporal dynamics and the well-being of the fetus, notably in terms of predicting the evolution of lactate, pH and cardiovascular decompensation (CVD). It makes uses of an established animal model of human labor, where 14 near-term ovine fetuses subjected to umbilical cord occlusions (UCO) were instrumented to permit regular intermittent measurements of metabolites lactate and base excess, pH, and continuous recording of electrocardiogram (ECG) and systemic arterial blood pressure (to identify CVD) during UCO. ECG-derived FHR was digitized at the sampling rate of 1,000 Hz and resampled to 4 Hz, as used in clinical routine. We focused on four FHR variability features which are tunable to temporal scales of FHR dynamics, robustly computable from FHR sampled at 4 Hz and within short-time sliding windows, hence permitting a time-dependent, or local, analysis of FHR which helps dealing with signal noise. Results show the sensitivity of the proposed features for early detection of CVD, correlation to metabolites and pH, useful for early acidosis detection and the importance of coarse time scales (2.5–8 s) which are not disturbed by the low FHR sampling rate. Further, we introduce the performance of an individualized self-referencing metric of the distance to healthy state, based on a combination of the four features. We demonstrate that this novel metric, applied to clinically available FHR temporal dynamics alone, accurately predicts the time occurrence of CVD which heralds a clinically significant degradation of the fetal health reserve to tolerate the trial of labor.

Strengths use is an essential personal resource to consider when designing higher-educational programs and interventions. Strengths use is associated with positive outcomes for both the student (e.g., study engagement) and the university (e.g., academic throughput/performance). The Strengths Use Scale (SUS) has become a popular psychometric instrument to measure strengths use in educational settings, yet its use has been subjected to limited psychometric scrutiny outside of the U.S. Further, its longitudinal stability has not yet been established. Given the wide use of this instrument, the goals of this study were to investigate (a) longitudinal factorial validity and the internal consistency of the scale, (b) its equivalence over time, and (c) criterion validity through its relationship with study engagement over time. Data were gathered at two-time points, 3 months apart, from a sample of students in the Netherlands (n = 360). Longitudinal confirmatory factor analyses showed support for a two-factor model for overall strengths use, comprised of Affinity for Strengths and Strengths Use Behaviors. The SUS demonstrated high levels of internal consistency at both the lower- and upper bound limits at both time points. Further, strict longitudinal measurement invariance was established, which confirmed the instrument's temporal stability. Finally, criterion validity was established through relating strengths use to study engagement at different time stamps. These findings support the use of the SUS in practice to measure strengths use and to track the effectiveness of strengths use interventions within the higher education sector.

Abstract Background Leaf senescence is a complex process, controlled by multiple genetic and environmental variables. In sunflower, leaf senescence is triggered abruptly following anthesis thereby limiting the capacity of plants to keep their green leaf area during grain filling, which subsequently has a strong impact on crop yield. Recently, we performed a selection of contrasting sunflower inbred lines for the progress of leaf senescence through a physiological, cytological and molecular approach. Here we present a large scale transcriptomic analysis using RNA-seq and its integration with metabolic profiles for two contrasting sunflower inbred lines, R453 and B481–6 (early and delayed senescence respectively), with the aim of identifying metabolic pathways associated to leaf senescence. Results Gene expression profiles revealed a higher number of differentially expressed genes, as well as, higher expression levels in R453, providing evidence for early activation of the senescence program in this line. Metabolic pathways associated with sugars and nutrient recycling were differentially regulated between the lines. Additionally, we identified transcription factors acting as hubs in the co-expression networks; some previously reported as senescence-associated genes in model species but many are novel candidate genes. Conclusions Understanding the onset and the progress of the senescence process in crops and the identification of these new candidate genes will likely prove highly useful for different management strategies to mitigate the impact of senescence on crop yield. Functional characterization of candidate genes will help to develop molecular tools for biotechnological applications in breeding crop yield.

This article presents experimental data describing the physiology and morphology of sunflower plants subjected to water deficit. Twenty-four sunflower genotypes were selected to represent genetic diversity within cultivated sunflower and included both inbred lines and their hybrids.Drought stress was applied to plants in pots at the vegetative stage using the high-throughput phenotyping platform Heliaphen at INRA Toulouse (France). Here, we provide data including specific leaf area, osmotic potential and adjustment, carbon isotope discrimination, leaf transpiration, plant architecture: plant height, leaf number, stem diameter. We also provide leaf areas of individual organs through time and growth rate during the stress period, environmental data such as temperatures, wind and radiation during the experiment. These data differentiate both treatment and the different genotypes and constitute a valuable resource to the community to study adaptation of crops to drought and the physiological basis of heterosis. It is available on the following repository: https://doi.org/10.25794/phenotype/er6lPW7V Keywords: Helianthus, Abiotic stress, Phenotyping platform, Drought stress

The present short commentary aimed at highlighting the potential impact of lockdown specifically on parents and children’s mental health. Embedded within a systemic view, it identified protective factors that may account for individual and household resilience. In the first section of this manuscript, we present the core concepts of resilience, before we highlight the adverse risks of the current COVID-19 situation on parents’ and children’s mental health. We provide evidence that parents and children alike might be concerned by increased risks of chronic diseases, abuse, and violence. Complementarily, we report the importance of psychological needs and competences and propose ways to promote these competencies among households. Overall, this paper emphasises that psychosocial competences such as creativity, emotion regulation, or interpersonal skills may constitute important characteristics to help parents and children alike coping and even thriving in the current worldwide adversity of the COVID-19 pandemic.

IntroductionPain management is the pillar of caring for patients with traumatic rib fractures. Intravenous lidocaine (IVL) is a well-established non-opioid analgesic for post-operative pain, yet its efficacy has yet to be investigated in trauma patients. We hypothesized that IVL is associated with decreased inpatient opioid requirements among patients with rib fractures.MethodsWe retrospectively evaluated adult patients presenting to our Level 1 trauma center with isolated chest wall injuries. After 1:1 propensity score matching patients who received vs did not receive IVL, we compared the two groups' average daily opioid use, opioid use in the last 24 hours of admission, and pain scores during admissions hours 24-48. We performed multivariable linear regression for these outcomes (with sensitivity analysis for the opioid use outcomes), adjusting for age as a moderating factor and controlling for hospital length of stay and injury severity.ResultsWe identified 534 patients, among whom 226 received IVL. Those who received IVL were older and had more serious injury. Compared to propensity-score matched patients who did not receive IVL, patients who received IVL had similar average daily opioid use and pain scores, but 40% lower opioid use during the last 24 hours of admission (p = 0.002). Multivariable regression-with and without sensitivity analysis-did not show an effect of IVL on any outcomes.ConclusionIVL was crudely associated with decreased opioid requirements in the last 24 hours of admission, the time period associated with opioid use at 90 days post-discharge. However, we did not observe beneficial effects of IVL on multivariable adjusted analyses; we are conducting a randomized control trial to further evaluate IVL's opioid-sparing effects for patients with rib fractures.

Opioid drugs are the gold standard for the management of pain, but their use is severely limited by dangerous and unpleasant side effects. All clinically available opioid analgesics bind to and activate the mu-opioid receptor (MOR), a heterotrimeric G-protein-coupled receptor, to produce analgesia. The activity of these receptors is modulated by a family of intracellular RGS proteins or regulators of G-protein signaling proteins, characterized by the presence of a conserved RGS Homology (RH) domain. These proteins act as negative regulators of G-protein signaling by serving as GTPase accelerating proteins or GAPS to switch off signaling by both the Gα and βγ subunits of heterotrimeric G-proteins. Consequently, knockdown or knockout of RGS protein activity enhances signaling downstream of MOR. In this review we discuss current knowledge of how this activity, across the different families of RGS proteins, modulates MOR activity, as well as activity of other members of the opioid receptor family, and so pain and analgesia in animal models, with particular emphasis on RGS4 and RGS9 families. We discuss inhibition of RGS proteins with small molecule inhibitors that bind to sensitive cysteine moieties in the RH domain and the potential for targeting this family of intracellular proteins as adjuncts to provide an opioid sparing effect or as standalone analgesics by promoting the activity of endogenous opioid peptides. Overall, we conclude that RGS proteins may be a novel drug target to provide analgesia with reduced opioid-like side effects, but that much basic work is needed to define the roles for specific RGS proteins, particularly in chronic pain, as well as a need to develop newer inhibitors.

Abstract Background Sexual parasites offer unique insights into the reproduction of unisexual and sexual populations. Because unisexuality is almost exclusively linked to the female sex, most studies addressed host-parasite dynamics in populations where sperm-dependent females dominate. Pelophylax water frogs from Central Europe include hybrids of both sexes, collectively named P. esculentus. They live syntopically with their parental species P. lessonae and/or P. ridibundus. Some hybrid lineages consist of all males providing a chance to understand the origin and perpetuation of a host-parasite (egg-dependent) system compared to sperm-dependent parthenogenesis. Methods We focused on P. ridibundus-P. esculentus populations where P. ridibundus of both sexes lives together with only diploid P. esculentus males. Based on 17 microsatellite markers and six allozyme loci, we analyzed (i) the variability of individual genomes, (ii) the reproductive mode(s) of all-male hybrids, and (iii) the genealogical relationships between the hybrid and parental genomes. Results Our microsatellite data revealed that P. esculentus males bear Mendelian-inherited ridibundus genomes while the lessonae genome represents a single clone. Our data indicate that this clone did not recently originate from adjacent P. lessonae populations, suggesting an older in situ or ex situ origin. Conclusions Our results confirm that also males can perpetuate over many generations as the unisexual lineage and successfully compete with P. ridibundus males for eggs provided by P. ridibundus females. Natural persistence of such sex-specific hybrid populations allows to studying the similarities and differences between male and female reproductive parasitism in many biological settings.

We introduce an index based on information theory to quantify the stationarity of a stochastic process. The index compares on the one hand the information contained in the increment at the time scale τ of the process at time t with, on the other hand, the extra information in the variable at time t that is not present at time t−τ. By varying the scale τ, the index can explore a full range of scales. We thus obtain a multi-scale quantity that is not restricted to the first two moments of the density distribution, nor to the covariance, but that probes the complete dependences in the process. This index indeed provides a measure of the regularity of the process at a given scale. Not only is this index able to indicate whether a realization of the process is stationary, but its evolution across scales also indicates how rough and non-stationary it is. We show how the index behaves for various synthetic processes proposed to model fluid turbulence, as well as on experimental fluid turbulence measurements.

Abstract Background Phoma macdonaldii has been reported as the causal agent of black stem disease (BS) and premature ripening (PR) on sunflower. PR is considered as the most widespread and detrimental disease on sunflower in France. While genetic variability and QTL mapping for partial resistance of sunflower to stem, collar and roots attacks have been reported on plantlets in controlled conditions, this work aims to describe the genetic variability in a subset of a sunflower lines, and for the first time to map QTL involved in PR resistance evaluated in field conditions using controlled inoculation. Results An efficient and reliable method for inoculation used in field experiments induced stem base necrosis on up to 98% of all plants. A significant genetic variability for PR resistance in the field was detected among the 20 inbred lines of the core collection tested across the two years. For QTL mapping, the PR resistance evaluation was performed on two recombinant inbred lines (RIL) populations derived from the crosses XRQxPSC8 and FUxPAZ2 in two different years. QTL analyses were based on a newly developed consensus genetic map comprising 1007 non-redundant molecular markers. In each of the two RIL populations, different QTL involved in PR partial sunflower resistance were detected. The most significant QTL were detected 49 days post infection (DPI) on LG10 (LOD 7.7) and on LG7 (LOD 12.1) in the XRQxPSC8 and FUxPAZ2 RIL population, respectively. In addition, different QTL were detected on both populations for PR resistance measured between 14 and 35 DPI. In parallel, the incidence of natural attack of P. macdonaldii resulting in BS disease was recorded, showing that in these populations, the genetic of resistance to both diseases is not governed by the same factors. Conclusion This work provides the first insights on the genetic architecture of sunflower PR resistance in the field. Moreover, the separate studies of symptoms on different organs and in time series allowed the identification of a succession of genetic components involved in the sunflower resistance to PR and BS diseases caused by Phoma macdonaldii along the development of the {plant * pathogen} interaction.