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High performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) is a potential method of choice for the analysis of carbohydrates., CARBOHYDRATES ARE ESSENTIAL to a wide range of industries, including food, pharmaceuticals, and consumer goods. Within the food industry, carbohydrates stand out as one of the key factors in determining [...]

Gnathia antennacrassa sp. nov. from seagrass beds off Rottnest Island, Western Australia is the first record of any gnathiid from the entirety of Western Australia; the male can be distinguished from congeners by the stout peduncular articles of the antenna. Gnathia taurus sp. nov. is described from two adult specimens reared from praniza larvae found infecting elasmobranch fishes at Heron Island, southern Great Barrier Reef; the males can be distinguished from all congeners by the dorsally strongly elongate mandibles and smoothly rounded mediofrontal process on the anterior part of cephalosome. Gnathia aff. maculosa Ota & Hirose, 2009 is recorded from Australia, together with further records of G. trimaculata Coetzee, Smit, Grutter & Davies, 2009 and G. grandilaris Coetzee, Smit, Grutter & Davies, 2008, all from elasmobranch fishes.

Abstract The vast majority of Parkinson’s disease cases are idiopathic. Unclear etiology and multifactorial nature complicate the comprehension of disease pathogenesis. Identification of early transcriptomic and metabolic alterations consistent across different idiopathic Parkinson’s disease (IPD) patients might reveal the potential basis of increased dopaminergic neuron vulnerability and primary disease mechanisms. In this study, we combine systems biology and data integration approaches to identify differences in transcriptomic and metabolic signatures between IPD patient and healthy individual-derived midbrain neural precursor cells. Characterization of gene expression and metabolic modeling reveal pyruvate, several amino acid and lipid metabolism as the most dysregulated metabolic pathways in IPD neural precursors. Furthermore, we show that IPD neural precursors endure mitochondrial metabolism impairment and a reduced total NAD pool. Accordingly, we show that treatment with NAD precursors increases ATP yield hence demonstrating a potential to rescue early IPD-associated metabolic changes.

Larval stages of the widely distributed digenean species Proctoeces maculatus (Looss, 1901) were reported 40 years ago from South Africa in the common octopus, Octopus vulgaris Cuvier (Octopodidae). However, the absence of adult specimens and molecular data from this region has hindered a comprehensive understanding of its distribution. In this study, we collected three species of intertidal and near-shore marine fishes [Clinus superciliosus (L.) (Clinidae), Diplodus capensis (Smith) (Sparidae) and Sparodon durbanensis (Castelnau) (Sparidae)] along the South African coast and discovered adult specimens of P. maculatus at five localities. By employing a combination of morphological and molecular techniques, including 28S rDNA, 18S rDNA and COI mtDNA analyses, the first report of adult P. maculatus from South Africa is presented. The findings encompass a comprehensive morphological description and molecular data, illuminating the true distribution of this species in the region.

During a parasitological survey carried out between May and August 2022 in the River Nyando, Lake Victoria Basin, a single species of Rhabdochona Railliet, 1916 (Nematoda: Rhabdochonidae) was recorded from the intestine of the Rippon barbel, Labeobarbus altianalis (Boulenger, 1900) (Cyprinidae). Based on light microscopy (LM), scanning electron microscopy (SEM) and DNA analyses the parasite was identified as Rhabdochona (Rhabdochona) gendrei Campana-Rouget, 1961. Light microscopy, SEM and DNA studies on this rhabdochonid resulted in a detailed redescription of the adult male and female. The following additional taxonomic features are described in the male: 14 anterior prostomal teeth; 12 pairs of preanal papillae: 11 subventral and one lateral; six pairs of postanal papillae: five subventral and one lateral, with the latter pair at the level of first subventral pairs when counted from the cloacal aperture. For the female: 14 anterior prostomal teeth and the size and absence of superficial structures on fully mature (larvated) eggs dissected out of the nematode body. Specimens of R. gendrei were genetically distinct in the 28S rRNA and cytochrome c oxidase subunit 1 (cox1) mitochondrial gene regions from known species of Rhabdochona. This is the first study that provides genetic data for a species of Rhabdochona from Africa, the first SEM of R. gendrei, and the first report of this parasite from Kenya. The molecular and SEM data reported herein provide a useful point of reference for future studies on Rhadochona in Africa.

Although parasites make up a substantial proportion of the biotic component of ecosystems, in terms of both biomass and number of species, they are rarely considered in conservation planning, except where they are thought to pose a threat to the conservation of their hosts. In this review, we address a number of unresolved questions concerning parasite conservation. Arguments for conserving parasite species refer to the intrinsic value conferred by their evolutionary heritage and potential, their functional role in the provision of ecosystem services, and their value as indicators of ecosystem quality. We propose that proper consideration of these arguments mean that it is not logically defensible to automatically exclude parasite species from conservation decisions; rather, endangered hosts and parasites should be considered together as a threatened ecological community. The extent to which parasites are threatened with extinction is difficult to estimate with any degree of confidence, because so many parasite species have yet to be identified and, even for those which have been formally described, we have limited information on the factors affecting their distribution and abundance. This lack of ecological information may partially explain the under-representation of parasites on threatened species lists. Effective conservation of parasites requires maintaining access to suitable hosts and the ecological conditions that permit successful transmission between hosts. When implementing recovery plans for threatened host species, this may be best achieved by attempting to restore the ecological conditions that maintain the host and its parasite fauna in dynamic equilibrium. Ecosystem-centred conservation may be a more effective strategy than species-centred (or host-parasite community-centred) approaches for preventing extinction of parasites, but the criteria which are typically used to identify protected areas do not provide information on the ecological conditions required for effective transmission. We propose a simple decision tree to aid the identification of appropriate conservation actions for threatened parasites.

Abstract While natural floods play a crucial role in shaping the composition of aquatic communities, the most rivers worldwide are regulated or dammed for anthropogenic purposes, resulting in alterations to the biological and chemical composition of these ecosystems. Studies have demonstrated various negative effects of river regulation on aquatic invertebrate communities globally. However, there is a scarcity of research in Africa, despite its vulnerability to anthropogenic impacts. This study aimed to compare aquatic invertebrate communities in the Phongolo River, an impacted regulated river, and the Usuthu River, a less impacted unregulated river, in South Africa. It further aimed to ascertain whether Lake Nyamithi, a naturally saline lake receiving water from both of the aforementioned systems, exhibited a stronger similarity to one of the two rivers in terms of its aquatic invertebrate composition. Aquatic invertebrate and water samples were collected from 2012 to 2018 over several surveys. The Usuthu River demonstrated a diverse and sensitive aquatic invertebrate community, emphasising its high conservation value. The Phongolo River demonstrated effects of anthropogenic impact, with taxa more resilient to changes in water quality and flow compared to the Usuthu River. Mismanagement and excessive water use may lead to the loss of any remaining sensitive aquatic invertebrate communities in this river. The presence of invasive molluscan in the Phongolo River and Lake Nyamithi also poses a threat to the native aquatic invertebrate communities. These invasive species are currently absent from the Usuthu River although other invasive species, such as the Australian redclaw crayfish, are found in both river systems. Lake Nyamithi displayed a unique aquatic invertebrate community, distinct from both rivers and their floodplains. This study provides important baseline information on the Usuthu River's aquatic invertebrates and emphasises the need to maintain adequate water flow in rivers and floodplains to protect biodiversity and sensitive species.

Clarias gariepinus (Burchell) is one of several freshwater fish species that have been translocated beyond its natural geographic range in South Africa. The present study investigated the parasitic communities of two translocated populations (one in the Riviersonderend River, Western Cape and the other from the Great Fish River, Eastern Cape) as well as its native source population from Gariep Dam, Free State. A total of nine, seven, and eight parasitic taxa were found to parasitise various organs of C. gariepinus from the three populations, respectively. The diversity and abundances of parasitic species in the two translocated populations were similar, but distinct community assemblages were observed. Parasite community composition from the Great Fish River was similar to that of the source population from Gariep Dam, whereas the parasitic community from C. gariepinus in the Riviersonderend River was distinct from that of Gariep Dam. This, together with the introduction history into the Western Cape, suggests that translocated C. gariepinus is sourced from various systems across South Africa, or that suitable intermediate hosts are present in the recipient ecosystems to sustain host-specific co-introduced parasitic taxa of C. gariepinus. In total, the resilience of 11 specialist parasite species of C. gariepinus is demonstrated in their persistence upon co-introduction into the two novel environments with their host, and support for the enemy release hypothesis is confirmed in the loss of known parasite taxa in translocated populations. The presence of the co-invasive fish lice Argulus japonicus Thiele, 1900 is reported from C. gariepinus in Gariep Dam and the Asian tapeworm Schyzocotyle acheilognathi (Yamaguti, 1934) was found from translocated C. gariepinus in the Riviersonderend River, Western Cape and lastly, a suspected case of parasite spillback from an unknown native host is reported.

A new species of Eniochobothrium Shipley and Hornell, 1906 was recovered from the Oman cownose ray (Rhinoptera jayakari Boulenger) from the body of water off the south-eastern coastline of the KwaZulu-Natal Province, South Africa. Eniochobothrium acostae n. sp. is described on morphological and molecular grounds. The new species is placed within Eniochobothrium (viz., Eniochobothrium gracile Shipley and Hornell, 1906, Eniochobothrium qatarense Al Kawari, Saoud and Wanas, 1994, Eniochobothrium euaxos Jensen, 2005) by possessing key generic characteristics such as the absence of a vagina, expansion of the anterior region of the strobila forming a trough and presence of a thick-walled cirrus sac. Molecular phylogenetic analyses of the partial 28S rRNA and mtCOI genes confirm the generic characterisation as the newly proposed species groups together with other members of the genus. Eniochobothrium acostae n. sp. currently represents the largest described species of the genus; it possesses slightly fewer testes compared to most congeners, given that this feature has been provided in the original description (e.g., E. euaxos and E. qatarense). The new species of Eniochobothrium is the fourth valid species described to date and the first species record from South African waters.

A new species, Gnathia pipinde sp. nov., is described from specimens taken from pufferfish, Amblyrhynchotes honckenii, at Chintsa and De Hoop Nature Reserve on the southern Indian Ocean coast of South Africa. Gnathia pipinde sp. nov. is characterised by the straight frontal margin, presence of conical superior frontolateral process, a strong and bifid mediofrontal processes, pronounced and pointed supraocular lobes, mandible strongly curved with a dentate blade, and the claviform penes produced more than a third the length of the pereon. A summary and key to the males of all known species of the Gnathiidae from the Temperate Southern African marine realm is provided.