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Background: Helicobacter pylori (H. pylori), a specific bacterium capable of surviving in the acidic environment of the stomach, has been recognized as a group of causative agents of gastric cancer. Therefore, the development of mucosal vaccines against H. pylori is expected to provide an important direction for the treatment of chronic gastritis and the prevention of gastric cancer. Methods and results: In this study, we used bacteria-like particles (BLPs) obtained by treating Lactic acid bacteria (L. lactis) with hot acid, and successfully displayed the M cell-targeted H. pylori multi-epitope purified antigen SAM-FAdE, with 90% display efficiency. In addition, BLPs-SAM-FAdE can effectively target M ​​cell models and M cells of mouse Peyer's patches (PPs) through oral immunization, promote the transport of particulate vaccines to dendritic cells (BMDCs) and stimulate their maturation, significantly increased proportion of plasma cells and germinal centers B cells. This indicates that the vaccination can induce notable antigen-specific mucosal immune responses (production of sIgA), CD4+ T cell responses (Th1/Th2/Th17) and humoral immune responses (production of serum IgG). Furthermore, oral BLPs-SAM-FAdE dramatically reduced the H. pylori adhesion and specific 16S rRNA expression of H. pylori in gastric mucosal tissue, protecting gastric tissue from damage. Conclusion: BLPs-SAM-FAdE can significantly reduce the adhesion of H. pylori in gastric mucosal tissue and inhibit gastritis and gastric damage caused by H. pylori infection. [ABSTRACT FROM AUTHOR]

Incorporation of nanosized metal organic frameworks (MOFs) fillers into polyamide layer can essentially enhance the performance of thin film nanocomposite (TFN). However, in situ growth of MOFs during interfacial polymerization (IP) is impeded by the insolubility of metal precursors in alkaline aqueous solutions. Herein, 1,4,7,10-tetraazacyclododecane (Cyclen), a macrocyclic polyamine capable of complexing metal ions, was integrated into the IP process to fabricate TFN membranes with in situ synthesized zeolitic imidazolate framework-8 (ZIF-8). Compared to the conventional piperazine-trimesoyl chloride (PIP-TMC) membrane, the modified PIP + Cyclen-TMC membrane features a thinner and denser polyamide layer. After the incorporation of ZIF-8, the polyamide layer becomes looser, more hydrophilic and electronegative. The resultant TFN membranes demonstrated exceptional water permeance (31.4 L m−2 h−1 bar−1) and improved selectivity (96.3 %), signifying a permeance enhancement of 182.6 % compared with that of the pristine PIP-TMC membrane. Additionally, the optimized membrane manifested enhanced fouling resistance and long-term stability. This macrocyclic polyamines mediated in situ growth technique can be extended to other combinations of macrocyclic polyamines and metal precursors, offering a novel paradigm for the fabrication of TFN-MOFs membranes for desalination. [Display omitted] • Macrocyclic polyamines were used to mediate the growth of MOFs during interfacial polymerization. • The introduction of macrocyclic polyamines (Cyclen) can effectively regulate the formation of polyamide layer. • In situ formed nanosized ZIF-8 were well dispersed into polyamide layer through the complexation between zinc and Cyclen. • The optimized membrane exhibited superior water permeance (31.4 L m−2 h−1 bar−1) and improved Na 2 SO 4 rejection (96.3 %). [ABSTRACT FROM AUTHOR]

Defect-engineered UiO-66-NH2 was introduced into a polyamide layer to form a thin film nanocomposite (TFN) membrane. Simultaneous enhancements in permeance and selectivity to Na2SO4 solution were achieved. [ABSTRACT FROM AUTHOR]