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2 results on '"Ngenazahayo A"'

1. Comparative characterization of stem cell marker expression, metabolic activity and resistance to doxorubicin in adherent and spheroid cells derived from the canine prostate adenocarcinoma cell line CT1258

Author

Wen, Liu, Mohammed, Moulay, Saskia, Willenbrock, Catrin, Roolf, Christian, Junghanss, Anaclet, Ngenazahayo, Ingo, Nolte, and Hugo, Murua Escobar

Subjects
Gene Expression Regulation, Neoplastic, Disease Models, Animal, Dogs, Doxorubicin, Drug Resistance, Neoplasm, Cell Line, Tumor, Spheroids, Cellular, Neoplastic Stem Cells, Animals, Humans, Integrin alpha6, Neoplasm Recurrence, Local
Abstract

Canine prostate cancer represents a spontaneous animal model for the human counterpart. Cells with stem cell-like character are considered to play a major role in therapeutic resistance and tumor relapse. Thus, the identification of markers allowing for recognition and characterization of these cells is essential.Expression of 12 stem cell marker genes in the canine prostate cancer cell line CT1258 and spheroid cells generated from these was analyzed by quantitative real-time PCR. In CT1258 and the generated spheroid cells, CD44 and CD133 expression was analyzed by flow cytometry, as well as proliferation and doxorubicin resistance.Integrin alpha-6 (ITGA6) expression and metabolic activity were significantly up-regulated in CT1258-derived spheroid cells, while doxorubicin resistance remained comparable.ITGA6 de-regulation and metabolic activity appear to be characteristic of the generated spheres, indicating potential intervention targets.

Published
2015

2. Comparative characterization of stem cell marker expression, metabolic activity and resistance to doxorubicin in adherent and spheroid cells derived from the canine prostate adenocarcinoma cell line CT1258.

Author

Liu W, Moulay M, Willenbrock S, Roolf C, Junghanss C, Ngenazahayo A, Nolte I, and Murua Escobar H

Subjects
Animals, Cell Line, Tumor, Disease Models, Animal, Dogs, Gene Expression Regulation, Neoplastic drug effects, Humans, Integrin alpha6 genetics, Neoplasm Recurrence, Local genetics, Neoplastic Stem Cells pathology, Spheroids, Cellular drug effects, Doxorubicin administration & dosage, Drug Resistance, Neoplasm genetics, Integrin alpha6 biosynthesis, Neoplasm Recurrence, Local drug therapy
Abstract

Background: Canine prostate cancer represents a spontaneous animal model for the human counterpart. Cells with stem cell-like character are considered to play a major role in therapeutic resistance and tumor relapse. Thus, the identification of markers allowing for recognition and characterization of these cells is essential., Materials and Methods: Expression of 12 stem cell marker genes in the canine prostate cancer cell line CT1258 and spheroid cells generated from these was analyzed by quantitative real-time PCR. In CT1258 and the generated spheroid cells, CD44 and CD133 expression was analyzed by flow cytometry, as well as proliferation and doxorubicin resistance., Results: Integrin alpha-6 (ITGA6) expression and metabolic activity were significantly up-regulated in CT1258-derived spheroid cells, while doxorubicin resistance remained comparable., Conclusion: ITGA6 de-regulation and metabolic activity appear to be characteristic of the generated spheres, indicating potential intervention targets., (Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.)

Published
2015

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