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10 results on '"Ng, Nadia"'

1. CSK-homologous kinase (CHK/MATK) is a potential colorectal cancer tumour suppressor gene epigenetically silenced by promoter methylation

Author

Chüeh, Anderly C., Advani, Gahana, Foroutan, Momeneh, Smith, Jai, Ng, Nadia, Nandurkar, Harshal, Lio, Daisy S., Zhu, Hong-Jian, Chong, Yuh-Ping, Verkade, Heather, Fujita, Donald J., Bjorge, Jeffrey, Basheer, Faiza, Lim, Jet Phey, Luk, Ian, Dhillon, Amardeep, Sakthianandeswaren, Anuratha, Mouradov, Dmitri, Sieber, Oliver, Hollande, Frédéric, Mariadason, John M., and Cheng, Heung-Chin

Published
2021
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2. CSK-Homologous Kinase

Author

Cheng, Heung-Chin, Advani, Gahana, Hossain, Mohammed Iqbal, Ng, Nadia L. Y., Lim, Ya Chee, Chüeh, Anderly C., Kamaruddin, Mohd Aizuddin, Chong, Yuh-Ping, and Choi, Sangdun, editor

Published
2018
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4. CSK-Homologous Kinase

Author

Cheng, Heung-Chin, primary, Advani, Gahana, additional, Hossain, Mohammed Iqbal, additional, Ng, Nadia LY, additional, Lim, Ya Chee, additional, Chüeh, Anderly C., additional, Kamaruddin, Mohd Aizuddin, additional, and Chong, Yuh-Ping, additional

Published
2016
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6. CSK-homologous kinase(CHK/MATK) is a potential colorectal cancer tumour suppressor gene epigenetically silenced by promoter methylation

Author

Chüeh, Anderly C., Advani, Gahana, Foroutan, Momeneh, Smith, Jai, Ng, Nadia, Nandurkar, Harshal, Lio, Daisy S., Zhu, Hong-Jian, Chong, Yuh-Ping, Verkade, Heather, Fujita, Donald J., Bjorge, Jeffrey, Basheer, Faiza, Lim, Jet Phey, Luk, Ian, Dhillon, Amardeep, Sakthianandeswaren, Anuratha, Mouradov, Dmitri, Sieber, Oliver, Hollande, Frédéric, Mariadason, John M., and Cheng, Heung-Chin

Abstract

Hyperactivation of SRC-family protein kinases (SFKs) contributes to the initiation and progression of human colorectal cancer (CRC). Since oncogenic mutations of SFKgenes are rare in human CRC, we investigated if SFK hyperactivation is linked to dysregulation of their upstream inhibitors, C-terminal SRC kinase (CSK) and its homolog CSK-homologous kinase (CHK/MATK). We demonstrate that expression of CHK/MATKbut not CSKwas significantly downregulated in CRC cell lines and primary tumours compared to normal colonic tissue. Investigation of the mechanism by which CHK/MATKexpression is down-regulated in CRC cells uncovered hypermethylation of the CHK/MATKpromoter in CRC cell lines and primary tumours. Promoter methylation of CHK/MATKwas also observed in several other tumour types. Consistent with epigenetic silencing of CHK/MATK, genetic deletion or pharmacological inhibition of DNA methyltransferases increased CHK/MATKmRNA expression in CHK/MATK-methylated colon cancer cell lines. SFKs were hyperactivated in CHK/MATK-methylated CRC cells despite expressing enzymatically active CSK, suggesting loss of CHK/MATK contributes to SFK hyperactivation. Re-expression of CHK/MATK in CRC cell lines led to reduction in SFK activity via a non-catalytic mechanism, a reduction in anchorage-independent growth, cell proliferation and migration in vitro, and a reduction in tumour growth and metastasis in a zebrafish embryo xenotransplantation model in vivo, collectively identifying CHK/MATKas a novel putative tumour suppressor gene in CRC. Furthermore, our discovery that CHK/MATKhypermethylation occurs in the majority of tumours warrants its further investigation as a diagnostic marker of CRC.

Published
2021
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7. Csk-homologous kinase (Chk) is an efficient inhibitor of Src-family kinases but a poor catalyst of phosphorylation of their C-terminal regulatory tyrosine

Author

Advani, Gahana, primary, Lim, Ya Chee, additional, Catimel, Bruno, additional, Lio, Daisy Sio Seng, additional, Ng, Nadia L. Y., additional, Chüeh, Anderly C., additional, Tran, Mai, additional, Anasir, Mohd Ishtiaq, additional, Verkade, Heather, additional, Zhu, Hong-Jian, additional, Turk, Benjamin E., additional, Smithgall, Thomas E., additional, Ang, Ching-Seng, additional, Griffin, Michael, additional, and Cheng, Heung-Chin, additional

Published
2017
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8. Additional file 14: Table S2. of Csk-homologous kinase (Chk) is an efficient inhibitor of Src-family kinases but a poor catalyst of phosphorylation of their C-terminal regulatory tyrosine

Author

Gahana Advani, Lim, Ya, Catimel, Bruno, Lio, Daisy, Ng, Nadia, Anderly ChĂźeh, Tran, Mai, Mohd Anasir, Verkade, Heather, Zhu, Hong-Jian, Turk, Benjamin, Smithgall, Thomas, Ching-Seng Ang, Griffin, Michael, and Heung-Chin Cheng

Subjects
animal structures, fungi, 3. Good health
Abstract

Identification of tryptic fragments derived from Src co- immunoprecipitated with Chk-GFP from the DLD-1-Chk-GFP cell lysate. Proteins immunoprecipitated from lysate of the DLD-1-Chk-GFP cells and that of the DLD-1-GFP cells (Control) were processed and digested with trypsin as described previously by Ang and Nice [56]. The tryptic fragments were analysed by LC-MS/MS as described in the experimental procedures. The SRC peptides identified by LC MS/MS and using the SEQUEST search engine are listed. A total of 4 different peptides were identified with high confidence at 1% FDR. The identify of these peptides were further validated with the Percolator algorithm for discrimination between correct and incorrect spectrum identifications as described by Kall, et al [84]. These fragments were not detected in the anti-GFP immunoprecipitate of the DLD-1-GFP cell lysate (Control lysate), suggesting that Src was specifically bound to Chk-GFP in the DLD-1-Chk-GFP cells. (PDF 93Â kb)

9. Additional file 14: Table S2. of Csk-homologous kinase (Chk) is an efficient inhibitor of Src-family kinases but a poor catalyst of phosphorylation of their C-terminal regulatory tyrosine

Author

Gahana Advani, Lim, Ya, Catimel, Bruno, Lio, Daisy, Ng, Nadia, Anderly ChĂźeh, Tran, Mai, Mohd Anasir, Verkade, Heather, Zhu, Hong-Jian, Turk, Benjamin, Smithgall, Thomas, Ching-Seng Ang, Griffin, Michael, and Heung-Chin Cheng

Subjects
animal structures, fungi, 3. Good health
Abstract

Identification of tryptic fragments derived from Src co- immunoprecipitated with Chk-GFP from the DLD-1-Chk-GFP cell lysate. Proteins immunoprecipitated from lysate of the DLD-1-Chk-GFP cells and that of the DLD-1-GFP cells (Control) were processed and digested with trypsin as described previously by Ang and Nice [56]. The tryptic fragments were analysed by LC-MS/MS as described in the experimental procedures. The SRC peptides identified by LC MS/MS and using the SEQUEST search engine are listed. A total of 4 different peptides were identified with high confidence at 1% FDR. The identify of these peptides were further validated with the Percolator algorithm for discrimination between correct and incorrect spectrum identifications as described by Kall, et al [84]. These fragments were not detected in the anti-GFP immunoprecipitate of the DLD-1-GFP cell lysate (Control lysate), suggesting that Src was specifically bound to Chk-GFP in the DLD-1-Chk-GFP cells. (PDF 93Â kb)

10. Additional file 8: Table S1. of Csk-homologous kinase (Chk) is an efficient inhibitor of Src-family kinases but a poor catalyst of phosphorylation of their C-terminal regulatory tyrosine

Author

Gahana Advani, Lim, Ya, Catimel, Bruno, Lio, Daisy, Ng, Nadia, Anderly ChĂźeh, Tran, Mai, Mohd Anasir, Verkade, Heather, Zhu, Hong-Jian, Turk, Benjamin, Smithgall, Thomas, Ching-Seng Ang, Griffin, Michael, and Heung-Chin Cheng

Subjects
animal structures, fungi, 3. Good health
Abstract

Determination of the molecular binding activity of Chk, Csk and Csk-Chk Chimera to immobilized Hck(2PA-YEEI) by surface plasmon spectroscopy. (PDF 447Â kb)

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