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Currently, the leatherback turtle population in Indonesia tends to decline. One of the factors that cause turtles' existence is the turtle eggs that fail to hatch because bacteria contaminate them, 80% of the reasons turtle eggs fail to hatch are due to infection by microorganisms found on the eggshell. Turtle eggs have a soft structure and there are pores that function for gas exchange and water absorption. Microorganisms in sand can infect turtle eggs through the pores and cause hatching failure. This study aims to isolate and identify pathogenic bacteria found in leatherback turtle eggshell (Dermochelys coriacea) from Lampuuk Beach, Aceh Besar, based on the 16S rRNA gene analysis. Eight eggshell samples from leatherback turtle eggs that failed and hatched were cultured on Nutrient Agar (NA) medium. Then, the samples were inoculated on a blood agar medium for haemolysis test. Molecular identification of the 16S rRNA gene was also carried out to determine bacterial species. A total of five bacterial colonies from leatherback turtle eggshells were successfully isolated consisting of two Gram-negative bacteria (Penyu Belimbing Aceh (PBA) = PBA-1 and PBA-2 and three Gram-positive bacteria (PBA-3, PBA-4, and PBA-5). Based on the haemolysis test, the five bacterial isolates were unable to haemolyze blood. Bacterial DNA of PBA-1 and PBA-2 were successfully isolated and amplified using polymerase chain reaction (PCR) with a DNA target of ~1500 bp. Analysis using BLASTN and phylogenetic tree construction showed that PBA-1 isolate had 98.64% similarity with Acinetobacter baumannii, while PBA-2 isolate had 97.82% similarity with Enterobacter kobei. Both bacteria are members of the Enterobacteriaceae family. It can be concluded that, there were two pathogenic bacteria can potentially be opportunistic pathogens found in leatherback turtle eggshells that failed or succeeded in hatching, namely A. baumannii and E. Kobei

The Remote Assessment of Disease and Relapse – Alzheimer’s Disease (RADAR-AD) consortium evaluated remote measurement technologies (RMTs) for assessing functional status in AD. The consortium engaged with the European Medicines Agency (EMA) to obtain feedback on identification of meaningful functional domains, selection of RMTs and clinical study design to assess the feasibility of using RMTs in AD clinical studies. We summarized the feedback and the lessons learned to guide future projects.

Portfolio formation is one of the strategies that investors can do to get the best results Portfolio formation can use the Downside Deviation method. The optimal portfolio with this method uses downside deviation and sets the return below the benchmark as a measure of risk. Every optimal portfolio certainly cannot be separated from risk. To measure risk, you can use the Value at Risk (VaR) and Expected Shortfall values. This study aims to form an optimal portfolio using the Downside Deviation method and continued by comparing the possible losses that occur from the formed portfolio using the VaR and Expected Shortfall values. The data used in this study is the daily closing price data of LQ-45 Index stocks in the banking sector in the period February-June 2023. From the stock data, data selection is carried out by selecting stocks that have a positive expected return and are normally distributed. Then, the optimal portfolio formation stage is continued using the Downside Deviation method and comparing the possible risks formed with the VaR and Expected Shortfall values. The results of this study show that the optimal portfolio with the Downside Deviation method consists of four stocks, namely with the stock codes BRIS.JK, BBRI.JK, BBNI.JK, and BBCA.JK. This study uses a case example by investing capital of Rp100,000,000 with a one-day time period and three levels of confidence, namely 90%, 95%, and 99%. Based on the comparison of the risk value of the portfolio formed using VaR and Expected Shortfall, it is shown that the possible risk with the Expected Shortfall method is greater than the VaR value. Therefore, Expected Shortfall is better in estimating the maximum risk.

Crude oil is a liquid characterized by a thick texture and blackish color. It is composed of complex hydrocarbon compounds with various benefits that are spread around the world. Crude oil derived from fossil fuels can be used as primary fuels, such as gasoline, and is the most important of the energy resources. Based on that, crude oil play a crucial role in the global economy movement because can be used as the main sources of energy all over the world. However, one of the benchmarks for crude oil from the USA is West Texas Intermediate (WTI). Known to produce high-quality oil, the price of crude oil of WTI fluctuates. In addition, fluctuations occur because of several factors, such as the availability of oil supplies, the embargo on oil imports, and the COVID-19 pandemic. The research aims to analyze price forecasting that occurs over the next five months and the accuracy level of the method used. The data that exists outliers is usually removed from forecasting that contains outliers, but that can affect the estimation result in the model. So, in this research intervention and outlier factors are added to the research to overcome the constraints In this study, the Multi-Input ARIMA Intervention and Additive Outlier (AO) method are used by modelling ARIMA pre-intervention and then. After that, the procedure is adding intervention factorsand additive outlier with iterative procedures. Multi-Input ARIMA Intervention and Additive Outlier (AO) are used to determine the magnitude of fluctuations that occur. Data shocks causing outlier data can be used by adding AO factors. WTI oil price data was retrieved from investing.com with monthly data from January 2011 to June 2023. Based on the results of Mmulti-Iinput ARIMA intervention with Additive Outlier method, it has been determined that the movement of WTI oil prices in the next five months will increase compared to the last five periods of actual data. Because of incrased price of crude oil, it will impact of the economic growth all over the world. So, the government better controlled the price of crude oil at lower price. . withMulti-Input ARIMA interventions resulting in AIC, MAPE, and RMSE model each 941.490, 6.979%, and 5.913 . So, Multi-Input and AO proven can be used to forecast data with fluctuate that data occur.

Introducción: La realidad virtual como estrategia de distracción es utilizada en los centros de atención en salud, sin embargo, la evidencia no es consistente en demostrar el efecto en el control del dolor durante el proceso de venopunción. Objetivo: Describir el efecto de la realidad virtual en el dolor durante el proceso de venopunción en niños y adolescentes, en una institución privada en la ciudad de Bogotá. Materiales y Métodos: Estudio experimental aleatorizado sin cegamiento. Se realizó en el servicio de urgencias de una institución de IV nivel de atención. La muestra estuvo constituida por 46 niños y adolescente entre los 7 y 14 años. De forma aleatoria 23 estuvieron en el grupo experimental con visor de realidad virtual y 23 en el grupo control. La variable dependiente dolor fue medido antes, durante y después de la venopunción con la escala análoga para el dolor. Resultados: El dolor percibido es diferente antes o después del procedimiento, sin embargo, el uso de gafas de realidad virtual no mostro diferencias estadísticas ni clínicas durante el proceso de venopunción. Discusión: Variables como los signos vitales, tiempo de venopunción y acompañamiento mostraron ser importantes cuando se miden síntomas como el dolor en la venopunción. Conclusión: La realidad virtual puede ser usada por profesionales de enfermería, sin embargo, debe investigarse más para demostrar efecto en el control del dolor durante la venopunción, teniendo en cuenta un mayor poder del estudio, tipo de dolor y variables como acompañamiento de familiares, tiempo de experiencia del enfermero.

Digital devices have revolutionized customer shopping behavior, giving rise to omni-channel retailing. Omni-channel retailing seamlessly integrates physical and digital channels, touchpoints, and supply chain operations. Owing to the rapidly evolving technology landscape, retailers struggle to discern the right investment approach to digitally transform their supply chains. Despite the widespread assumption that investment in supply chain integration (SCI) is vital for omni-channel retailing, empirical studies investigating the underlying mechanisms behind this process are limited. This research seeks to understand how and why investment ability catalyzes omni-channel SCI. Utilizing a two-phase abductive qualitative research design, Phase 1 involved 17 semi-structured interviews with senior managers from nine large South African omni-channel retailers. In Phase 2, content analysis of 40 integrated annual reports (IARs) from omni-channel retailers was conducted. This study identifies two crucial investment trade-off decisions that drive omni-channel SCI: financial and human resources. This demonstrates that strategically aligned investment ability fosters scalability and agility, playing a catalytic role in omni-channel SCI. The findings contribute valuable insights into omni-channel retailing and supply chain literature, providing practical guidance for managers navigating the complexities of investing in omni-channel SCI initiatives. These investment guidelines could accelerate the digital transformation and SCI processes for omni-channel retailers.

Abstract B cells and T cells collaborate in multiple sclerosis (MS) pathogenesis. IgH[MOG] mice possess a B cell repertoire skewed to recognize myelin oligodendrocyte glycoprotein (MOG). Here, we show that upon immunization with the T cell-obligate autoantigen, MOG[35-55], IgH[MOG] mice develop rapid and exacerbated experimental autoimmune encephalomyelitis (EAE) relative to wildtype (WT) counterparts, characterized by aggregation of T and B cells in the IgH[MOG] meninges and by CD4+ T helper 17 (Th17) cells in the CNS. Production of the Th17 maintenance factor IL-23 is observed from IgH[MOG] CNS-infiltrating and meningeal B cells, and in vivo blockade of IL-23p19 attenuates disease severity in IgH[MOG] mice. In the CNS parenchyma and dura mater of IgH[MOG] mice, we observe an increased frequency of CD4+PD-1+CXCR5- T cells that share numerous characteristics with the recently described T peripheral helper (Tph) cell subset. Further, CNS-infiltrating B and Tph cells from IgH[MOG] mice show increased reactive oxygen species (ROS) production. Meningeal inflammation, Tph-like cell accumulation in the CNS and B/Tph cell production of ROS were all reduced upon p19 blockade. Altogether, MOG-specific B cells promote autoimmune inflammation of the CNS parenchyma and meninges in an IL-23-dependent manner.

Introduction: Salbutamol is a moderately selective beta-2-adrenergic agonist. Various side effects can occur because of beta-1 and beta-2 receptor activation. Due to the large volume of distribution, it is not considered dialyzable. Case Presentation: A patient with salbutamol intoxication, which developed as a result of a medical error in a patient with sepsis, Down syndrome, and liver cirrhosis, is presented. Initial treatment was partially successful and antibiotic adjustments were made. After his respiratory failure worsened, the patient needed non-invasive ventilation, and previously undiagnosed chronic obstructive pulmonary disease was suspected. He was prescribed intravenous methylprednisolone but accidently received 5 mg of salbutamol (albuterol), which led to immediate severe arrhythmic tachycardia with hemodynamic collapse. After unsuccessful cardioversion and treatment with landiolol infusion, salvage hemodialysis was commenced to decrease suspectedly highly elevated serum salbutamol levels. After 30 min, sinus rhythm with normocardia was observed. After the hemodialysis termination, no rebound tachycardia was noted, but due to severe septic shock, the hypotension was ongoing and vasoactive medications were adjusted. However, the measured levels of plasma salbutamol and data from literature do not support the view that hemodialysis was the cause of the described improvement: the total amount of the drug cleared was very small (2.8% of total dose). Conclusion: Our results confirm a large volume of salbutamol distribution; the measured levels are within observed therapeutic levels; and the measured half-life time during hemodialysis (3.1 h) is comparable to observed half-life times in therapeutic settings. The observed favorable clinical benefit associated with dialysis may be fortuitous, highlighting potential bias toward positive clinical outcomes and unproven (“salvage”) therapies.

Anticancer Potential of Watermelon Seed Extracts Against Lung and Breast Cancer Cell LinesKarpuz Çekirdeği Ekstrelerinin Akciğer ve Meme Kanseri Hücre Hatlarına Karşı Antikanser PotansiyeliRunning Title: Anticancer Potential of Watermelon SeedAbstractObjective: The aim of the study is to evaluate the anticancer potential of watermelon seed extracts against lung and breast cancer cell lines. Methods: A549 lung cancer and MCF-7 breast cancer cell lines were used. The cells were treated with doses ranging from 0.1 to 1000 µg/mL of KI and KD extracts from watermelon seeds, starting when the cell density reached 80%. Viability was assessed using the MTT assay.Results: For the A549 lung cell line, the KI extract demonstrated significant anticancer activity at doses of 10, 100, and 1000 µg/mL, with the 1000 µg/mL dose being the most effective against lung cancer cells. Similarly, the KD extract showed efficacy across all doses tested, with the seed peel being effective at lower doses compared to the seed. In the MCF-7 breast cell line, both KI and KD extracts exhibited dose-dependent anticancer effects, with significant reductions in viability observed at all doses compared to the control group. Conclusion: Interestingly, the seed and seed shell showed selective effectiveness against breast and lung cancer, indicating a dose-dependent and selective anticancer effect. Overall, these findings suggest the potential of watermelon seed extracts as promising anticancer agents with selective efficacy against different cancer types. Keywords: Watermelon, anticancer, A549 lung cancer cell, MCF-7 breast cancer cellÖZAmaç: Çalışmanın amacı, karpuz çekirdeği ekstrelerinin akciğer ve meme kanseri hücre hatlarına karşı antikanser potansiyelini değerlendirmektir.Yöntemler: A549 akciğer kanseri ve MCF-7 meme kanseri hücre hatları kullanılmıştır. Hücreler, yoğunlukları %80'e ulaştığında, karpuz çekirdeğinden hazırlanan KI ve KD ekstreleri ile 0.1 ila 1000 µg/mL doz aralığında muamele edilmiştir. Canlılık, MTT testi kullanılarak değerlendirilmiştir.Bulgular: A549 akciğer hücre hattı için KI ekstresi, 10, 100 ve 1000 µg/mL dozlarında önemli antikanser aktivite göstermiş, 1000 µg/mL dozu akciğer kanseri hücrelerine karşı en etkili olarak bulunmuştur. Benzer şekilde, KD ekstresi, test edilen tüm dozlarda etkili olmuş ve tohum kabuğunun, tohuma göre daha düşük dozlarda etkili olduğu görülmüştür. MCF-7 meme hücre hattında, hem KI hem de KD ekstreleri doza bağlı antikanser etki sergilemiş ve kontrol grubuna kıyasla tüm dozlarda önemli düzeyde canlılık azalması gözlenmiştir.Sonuç: Karpuz çekirdeği ve kabuğu meme ve akciğer kanserine karşı seçici etkinlik göstermiş, bu da doza bağlı ve seçici bir antikanser etkiyi işaret etmektedir. Genel olarak, bu bulgular, karpuz çekirdeği ekstrelerinin farklı kanser türlerine karşı seçici etkili ve umut vaat edici antikanser ajanlar olarak potansiyelini göstermektedir.Anahtar kelimeler: Karpuz, antikanser, A549 akciğer kanseri hücre, MCF-7 meme kanseri hücre

Time-resolved step-scan Fourier transform infrared difference spectroscopy has been used to study cyanobacterial photosystem I photosynthetic reaction centers from Synechocystis sp. PCC 6803 (S6803) with four high-potential, 1,4-naphthoquinones incorporated into the A1 binding site. The high-potential naphthoquinones are 2-chloro-, 2-bromo-, 2,3-dichloro- and 2,3-dibromo-1,4-naphthoquinone. "Foreign minus native" double difference spectra (DDS) were constructed by subtracting difference spectra for native photosystem I (with phylloquinone in the A1 binding site) from corresponding spectra obtained using photosystem I with the different quinones incorporated. To help assess and assign bands in the difference and double difference spectra, density functional theory based vibrational frequency calculations for the different quinones in solvent, or in the presence of a single asymmetric H- bond to either a water molecule or a peptide backbone NH group, were undertaken. Calculated and experimental spectra agree best for the peptide backbone asymmetrically H- bonded system. By comparing multiple sets of double difference spectra, several new bands for the native quinone (phylloquinone) are identified. By comparing calculated and experimental spectra we conclude that the mono-substituted halogenated NQs can occupy the binding site in either of two different orientations, with the chlorine or bromine atom being either ortho or meta to the H- bonded CO group.

Abstract Background and Aims The purpose of this study was to compare the knowledge and practices of specialist and experienced nonspecialist physical therapists in performing patient education about physical activity with patients with heart failure (HF). Methods Responses on a nationwide anonymous online survey were used to compare specialist and experienced nonspecialist physical therapists on knowledge and frequency of providing physical activity related education to patients hospitalized with acutely decompensated HF. Responses to survey items were scored on 5‐point scales ranging from “Strongly agree” to “Strongly disagree” or “Always” to “Never.” Mann−Whitney U statistics were used to compare specialist and experienced nonspecialist responses and Wilcoxon signed‐ranks tests were used to examine the gap between knowledge and practice. Results Twenty‐seven specialists and 43 experienced nonspecialists completed the survey. Both groups were similar in age, and experience treating patients hospitalized with acutely decompensated HF. Both groups “strongly agree” that they had the required knowledge and skills to educate patients with HF on the physical activity topics. However, specialists more often than experienced nonspecialists provided education on topics such as how to monitor vital signs during physical activity (“most of the time” vs. “about half of the time”) that promoted patient confidence and safety during exercise. Specialists demonstrated a smaller gap between knowledge and frequency of providing patient education than experienced nonspecialists on three of the four patient education topics. Conclusion Specialist physical therapists treating patients with HF in the inpatient hospital setting provided patient education on physical activity at a level more closely matching their skills and the clinical practice guideline than did experienced nonspecialists. Physical therapy clinical specialists practicing in the inpatient hospital setting may improve patient outcomes and lower costs to the health care system by improving physical activity adherence and thereby may reduce avoidable hospital readmissions.

Abstract Background Papillary carcinoma is the most frequent type of thyroid carcinoma, while primary thyroid lymphoma is uncommon disease. The coexistence of these entities has already been described, and the common risk factor is considered Hashimoto thyroiditis. The two most frequent histotypes of primary thyroid lymphoma are diffuse large B-cell and mucosa-associated lymphoid tissue lymphoma, but the coexistence of both with papillary carcinoma is rarely reported. Methods We present a case of a previously healthy 57-years old male with rapidly growing lump on the right side of the neck. Ultrasonography revealed nodules in both thyroid lobes. Fine needle aspiration cytology and pertechnetate scintigraphy were performed. Due to the Bethesda T-5 in the “cold” nodule of the right lobe, surgery with histopathological and immunohistochemistry analysis was indicated. Results Histopathological and immunohistochemistry methods confirmed concomitant malignancies in the thyroid gland: diffuse large B-cell lymphoma and papillary carcinoma in the right, and mucosa-associated lymphoid tissue lymphoma in the left lobe with Hashimoto thyroiditis in the remaining tissue. Patient underwent therapy procedures and was without signs of local recurrence or metastatic spread on subsequent follow-up. Conclusions Sudden appearance of the neck mass in patients with Hashimoto thyroiditis should raise suspicion on primary thyroid lymphoma and be promptly taken in the diagnostic workup, including fine needle aspiration cytology. Pathology with immunohistochemistry is crucial for further clinical decision making. Since the standardized protocol in management of these complex patients is missing, personal approach and close collaboration between cytologist, pathologist, surgeon, haematologist and nuclear medicine specialist is essential.

Indonesia's export activities are dominated by non-oil and gas exports consisting of four sectors, namely the processing industry, agriculture, mining, and others. The government must pay attention to non-oil and gas exports for each province because exports can play an essential role in a country's economic growth. This study was conducted to cluster provinces in Indonesia using time series clustering in the non-oil and gas export sector based on data patterns concerning Dynamic Time Warping (DTW) distance. The sectors used in this study are the manufacturing industry sector and the agricultural sector in 34 Indonesian provinces in the period 2017 - 2021. Time series clustering analysis uses the average linkage method with DTW distance and the selection of the optimum number of clusters using the silhouette coefficient method. The results of the analysis in the processing industry sector resulted in 3 optimum clusters, namely cluster 1 consisting of 1 province that has high processing industry exports, cluster 2 consisting of 8 provinces that have medium processing industry exports, and cluster 3 consisting of 25 provinces that have low processing industry exports. As for the agricultural sector, it produces 2 optimum clusters, namely cluster 1 consisting of 5 provinces that have high agricultural industry exports, and cluster 2 consisting of 29 provinces that have low agricultural industry exports. The clustering results in the processing industry sector and the agricultural sectors have a silhouette coefficient value of 0.778 and 0.798, so it is said to have a strong cluster structure.

Za razliku od klasičnih računalnih programa koji funkcioniraju na temelju eksplicitno definiranih instrukcija koje im programer daje, umjetna inteligencija (engl. artificial intelligence – AI) temeljena na strojnom učenju (engl. machine learning) i korištenju neuralnih mreža (engl. neural networking) može samostalno poboljšavati svoje performanse kako dobiva više podataka, odnosno ima sposobnost učenja. Takvi sustavi u radiologiji trenirani su na ogromnim skupovima podataka kako bi prepoznali patološke promjene na radiološkim snimkama uključujući tumore, frakture i druge abnormalnosti. AI sustavi u medicini dramatično mijenjaju način kako se dijagnosticiraju i liječe bolesti. Strojno učenje omogućava naprednu analizu medicinskih slika, točnije identificirajući patologiju. Ovi sustavi, nadmašujući tradicionalne metode, ubrzavaju dijagnostički proces i unapređuju kvalitetu slika, što dovodi do bržih i preciznijih dijagnoza. Primjena AI-a omogućuje i bolje praćenje pacijenata te predviđanje tijeka bolesti, doprinoseći personaliziranom pristupu liječenju. Međutim, izazovi poput integracije u zdravstvene sustave, obrade privatnosti i podataka, te etičkih pitanja predstavljaju prepreke za širu primjenu. Postoji potreba za rigoroznom validacijom AI algoritama, kao i za obukom medicinskog osoblja u korištenju ovih tehnologija. Unatoč ovim izazovima, budućnost AI-a u radiologiji izgleda obećavajuće. Očekuje se daljnji razvoj preciznijih i efikasnijih AI algoritama te njihova integracija s drugim medicinskim inovacijama. To će voditi ka boljem praćenju pacijenata i pružanju personalizirane skrbi. AI u radiologiji ima potencijal ne samo za poboljšanje kvalitete zdravstvene skrbi, već i za transformaciju načina na koji se skrb pruža, s naglaskom na pažljivu regulaciju i kontinuiranu edukaciju kako bi se maksimizirale koristi i minimizirali rizici.

Salmonella Infantis poses a significant challenge in poultry production due to its persistence and resistance to disinfectants. This study investigated the survival of the S. Infantis strain on different surfaces and evaluated the efficacy of disinfectants in both preventing and treating biofilms. The survival of the tested S. Infantis strain was assessed on plastic and stainless steel surfaces after 24 and 48 h. The minimum inhibitory concentrations (MICs) of five disinfectants were determined, and their antiadhesion effectiveness was evaluated using crystal violet. The efficacy of biofilm treatment was evaluated by cell culturability. The results showed that the adhesion of S. Infantis was significantly higher on the plastic surface. The disinfectants were effective at reducing biofilm formation only within the first 24 h. Fresh solutions of disinfectants based on quaternary ammonium compounds exhibited the highest antimicrobial efficacy, while chlorocresol was the most effective for both the prevention and treatment of biofilms. The study results suggest that the presence of plastic surfaces may contribute to the dissemination of Salmonella. Additionally, the effectiveness of disinfectants varied based on storage conditions and contact time, while biofilms demonstrated reduced susceptibility compared to planktonic cells. However, given the laboratory scale of this study, further validation on a commercial scale is necessary to confirm these findings.

Children and adolescents (C&As) with congenital heart defects (CHDs) have decreased functional capacity and executive functioning (EF) due to brain abnormalities and decreased cerebral perfusion. Exercise may improve EF via increased cognitive demands and cerebral blood supply. The purpose of this review was to identify evidence describing the impact of physical activity (PA) interventions on EF in C&As with CHDs. The following databases were searched from 2000 to 2024: MEDLINE, EMBASE, CINAHL, Scopus, CENTRAL, and PsycInfo. The inclusion criteria consisted of participants aged from birth to 18 years with CHD, interventions related to PA, and EF as an outcome measure. Articles were excluded if adults were included, translation to English was impossible, and full access was unavailable. Of 613 initial articles, 3 were analyzed, with only 1 meeting all inclusion criteria. The included study found significant improvements in self-reported cognitive functioning and parent-reported social functioning after 12 weeks of aerobic exercise in children aged 10–15 years with CHDs. Common themes among the reviewed articles indicated that EF remains impaired throughout the lifespan, children have unique interventional and developmental needs, and research remains limited despite theoretical benefits. Further investigation of the effect of PA on EF in C&As with CHDs is needed.

Background and objectivesIt is difficult to predict post-stroke outcome for people with severe motor impairment, as both clinical tests and corticospinal tract (CST) microstructure may not reliably indicate severe motor impairment. Here, we test whether imaging biomarkers beyond the CST relate to severe upper limb impairment post-stroke by evaluating white matter microstructure in the corpus callosum (CC). In an international, multisite hypothesis-generating observational study we determined if: a) CST asymmetry index can differentiate between individuals with mild-moderate and severe upper limb impairment; and b) CC biomarkers relate to upper limb impairment within individuals with severe impairment post-stroke. We hypothesised that CST asymmetry index would differentiate between mild-moderate and severe impairment, but CC microstructure would relate to motor outcome for individuals with severe upper limb impairment.MethodsSeven cohorts with individual diffusion imaging and motor impairment (Fugl Meyer-Upper Limb) data were pooled. Hand-drawn regions-of-interest were used to seed probabilistic tractography for CST (ipsilesional/contralesional) and CC (prefrontal/premotor/motor/sensory/posterior) tracts. Our main imaging measure was mean fractional anisotropy. Linear mixed-effect regression explored relationships between candidate biomarkers and motor impairment, controlling for observations nested within cohorts, as well as age, sex, time post-stroke and lesion volume.ResultsData from 110 individuals (30 mild-moderate, 80 with severe motor impairment) were included. In the full sample, greater CST asymmetry index (i.e., lower fractional anisotropy in the ipsilesional hemisphere, p

Interventions focused on the home language environment have been shown to improve a number of child language outcomes in the first years of life. However, data on the longer-term effects of the intervention are still somewhat limited. The current study examines child vocabulary and complex speech outcomes (N = 59) during the year following completion of a parent-coaching intervention, which was previously found to increase the quantity of parent-child conversational turns and to improve child language outcomes through 18 months of age. Measures of parental language input, child speech output, and parent-child conversational turn-taking were manually coded from naturalistic home recordings (Language Environment Analysis System, LENA) at regular 4-month intervals when children were 6- to 24-months old. Child language skills were assessed using the MacArthur-Bates Communicative Development Inventory (CDI) at four time-points following the final intervention session (at 18, 24, 27, and 30 months). Vocabulary size and growth from 18 to 30 months was greater in the intervention group, even after accounting for differences in child language ability during the intervention period. The intervention group also scored higher on measures of speech length and grammatical complexity, and these effects were mediated by 18-month vocabulary. Intervention was associated with increased parent-child conversational turn-taking in home recordings at 14 months, and mediation analysis suggested that 14-month conversational turn-taking accounted for intervention-related differences in subsequent vocabulary. Together, the results suggest enduring, positive effects of parental language intervention and underscore the importance of interactive, conversational language experience during the first 2 years of life.

This study engages readers in a dialogue on redesigning retention strategies from reactive to proactive, a conceptualization of interdependencies of retention intervention metrics and student behaviors, and identifies the actions needed to achieve them in practice through real-time collaborative campus partnerships. Best practices for data collection and analysis, including anticipated impacts on specific workflows and student support services, are discussed through a multi-pronged and multi-phased approach to allow for predictive modeling. While this study explores model development for Kean University, a four-year public university in New Jersey, it has implications for replication at other institutions to increase persistence, retention, and timely graduation.

TDP-43 (TAR DNA-binding protein 43) aggregation and redistribution are recognised as a hallmark of amyotrophic lateral sclerosis and frontotemporal dementia. As TDP-43 inclusions have recently been described in the muscle of inclusion body myositis patients, this highlights the need to understand the role of TDP-43 beyond the central nervous system. Using RNA-seq, we directly compare TDP-43-mediated RNA processing in muscle (C2C12) and neuronal (NSC34) mouse cells. TDP-43 displays a cell-type-characteristic behaviour targeting unique transcripts in each cell-type, which is due to characteristic expression of RNA-binding proteins, that influence TDP-43's performance and define cell-type specific splicing. Among splicing events commonly dysregulated in both cell lines, we identify some that are TDP-43-dependent also in human cells. Inclusion levels of these alternative exons are altered in tissues of patients suffering from FTLD and IBM. We therefore propose that TDP-43 dysfunction contributes to disease development either in a common or a tissue-specific manner.