22 results on '"Neupane J"'
Search Results
2. A systematic analysis of the suitability of preimplantation genetic diagnosis for mitochondrial diseases in a heteroplasmic mitochondrial mouse model
- Author
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Neupane, J., primary, Vandewoestyne, M., additional, Heindryckx, B., additional, Ghimire, S., additional, Lu, Y., additional, Qian, C., additional, Lierman, S., additional, Van Coster, R., additional, Gerris, J., additional, Deroo, T., additional, Deforce, D., additional, and De Sutter, P., additional
- Published
- 2014
- Full Text
- View/download PDF
3. Reproductive (epi)genetics
- Author
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Lynch, C., primary, Tee, N., additional, Rouse, H., additional, Gordon, A., additional, Sati, L., additional, Zeiss, C., additional, Soygur, B., additional, Bassorgun, I., additional, Goksu, E., additional, Demir, R., additional, McGrath, J., additional, Groendahl, M. L., additional, Thuesen, L., additional, Andersen, A. N., additional, Loft, A., additional, Smitz, J., additional, Adriaenssens, T., additional, Vikesa, J., additional, Borup, R., additional, Mersy, E., additional, Kisters, N., additional, Macville, M. V. E., additional, Engelen, J. J. M., additional, Consortium, S.-E. N. N., additional, Menheere, P. P. C. A., additional, Geraedts, J. P., additional, Coumans, A. B. C., additional, Frints, S. G. M., additional, Aledani, T., additional, Assou, S., additional, Traver, S., additional, Ait-ahmed, O., additional, Dechaud, H., additional, Hamamah, S., additional, Mizutani, E., additional, Suzumori, N., additional, Sugiyama, C., additional, Hattori, Y., additional, Sato, T., additional, Ando, H., additional, Ozaki, Y., additional, Sugiura-Ogasawara, M., additional, Wissing, M., additional, Kristensen, S. G., additional, Andersen, C. Y., additional, Mikkelsen, A. L., additional, Hoest, T., additional, Velthut-Meikas, A., additional, Simm, J., additional, Metsis, M., additional, Salumets, A., additional, Palini, S., additional, Galluzzi, L., additional, De Stefani, S., additional, Primiterra, M., additional, Wells, D., additional, Magnani, M., additional, Bulletti, C., additional, Vogt, P. H., additional, Frank-Herrmann, P., additional, Bender, U., additional, Strowitzki, T., additional, Besikoglu, B., additional, Heidemann, P., additional, Wunsch, L., additional, Bettendorf, M., additional, Jelinkova, L., additional, Vilimova, S., additional, Kosarova, M., additional, Sebek, P., additional, Volemanova, E., additional, Kruzelova, M., additional, Civisova, J., additional, Svobodova, L., additional, Sobotka, V., additional, Mardesic, T., additional, van de Werken, C., additional, Santos, M. A., additional, Eleveld, C., additional, Laven, J. S. E., additional, Baart, E. B., additional, Pylyp, L. Y., additional, Spinenko, L. A., additional, Zukin, V. D., additional, Perez-Sanz, J., additional, Matorras, R., additional, Arluzea, J., additional, Bilbao, J., additional, Gonzalez-Santiago, N., additional, Yeh, N., additional, Koff, A., additional, Barlas, A., additional, Romin, Y., additional, Manova-Todorova, K., additional, Hoz, C. D. l., additional, Mauri, A. L., additional, Nascimento, A. M., additional, Vagnini, L. D., additional, Petersen, C. G., additional, Ricci, J., additional, Massaro, F. C., additional, Cavagna, M., additional, Pontes, A., additional, Oliveira, J. B. A., additional, Baruffi, R. L. R., additional, Franco, J. G., additional, Wu, E. X., additional, Ma, S., additional, Parriego, M., additional, Sole, M., additional, Boada, M., additional, Coroleu, B., additional, Veiga, A., additional, Kakourou, G., additional, Poulou, M., additional, Vrettou, C., additional, Destouni, A., additional, Traeger-Synodinos, J., additional, Kanavakis, E., additional, Yatsenko, A. N., additional, Georgiadis, A. P., additional, McGuire, M. M., additional, Zorrilla, M., additional, Bunce, K. D., additional, Peters, D., additional, Rajkovic, A., additional, Olszewska, M., additional, Kurpisz, M., additional, Gilbertson, A. Z. A., additional, Ottolini, C. S., additional, Summers, M. C., additional, Sage, K., additional, Handyside, A. H., additional, Thornhill, A. R., additional, Griffin, D. K., additional, Chung, M. K., additional, Kim, J. W., additional, Lee, J. H., additional, Jeong, H. J., additional, Kim, M. H., additional, Ryu, M. J., additional, Park, S. J., additional, Kang, H. Y., additional, Lee, H. S., additional, Zimmermann, B., additional, Banjevic, M., additional, Hill, M., additional, Lacroute, P., additional, Dodd, M., additional, Sigurjonsson, S., additional, Lau, P., additional, Prosen, D., additional, Chopra, N., additional, Ryan, A., additional, Hall, M., additional, McAdoo, S., additional, Demko, Z., additional, Levy, B., additional, Rabinowitz, M., additional, Vereczeky, A., additional, Kosa, Z. S., additional, Savay, S., additional, Csenki, M., additional, Nanassy, L., additional, Dudas, B., additional, Domotor, Z. S., additional, Debreceni, D., additional, Rossi, A., additional, Alegretti, J. R., additional, Cuzzi, J., additional, Bonavita, M., additional, Tanada, M., additional, Matunaga, P., additional, Fettback, P., additional, Rosa, M. B., additional, Maia, V., additional, Hassun, P., additional, Motta, E. L. A., additional, Piccolomini, M., additional, Gomes, C., additional, Barros, B., additional, Nicoliello, M., additional, Criscuolo, T., additional, Miyadahira, E., additional, Montjean, D., additional, Benkhalifa, M., additional, Berthaut, I., additional, Griveau, J. F., additional, Morcel, K., additional, Bashamboo, A., additional, McElreavey, K., additional, Ravel, C., additional, Rubio, C., additional, Rodrigo, L., additional, Mateu, E., additional, Mercader, A., additional, Peinado, V., additional, Buendia, P., additional, Milan, M., additional, Delgado, A., additional, Al-Asmar, N., additional, Escrich, L., additional, Campos-Galindo, I., additional, Garcia-Herrero, S., additional, Poo, M. E., additional, Mir, P., additional, Simon, C., additional, Reyes-Engel, A., additional, Cortes-Rodriguez, M., additional, Lendinez, A., additional, Perez-Nevot, B., additional, Palomares, A. R., additional, Galdon, M. R., additional, Ruberti, A., additional, Minasi, M. G., additional, Biricik, A., additional, Colasante, A., additional, Zavaglia, D., additional, Iammarrone, E., additional, Fiorentino, F., additional, Greco, E., additional, Demir, N., additional, Ozturk, S., additional, Sozen, B., additional, Morales, R., additional, Lledo, B., additional, Ortiz, J. A., additional, Ten, J., additional, Llacer, J., additional, Bernabeu, R., additional, Nagayoshi, M., additional, Tanaka, A., additional, Tanaka, I., additional, Kusunoki, H., additional, Watanabe, S., additional, Temel, S. G., additional, Beyazyurek, C., additional, Ekmekci, G. C., additional, Aybar, F., additional, Cinar, C., additional, Kahraman, S., additional, Nordqvist, S., additional, Karehed, K., additional, Akerud, H., additional, Gultomruk, M., additional, Tulay, P., additional, Findikli, N., additional, Yagmur, E., additional, Karlikaya, G., additional, Ulug, U., additional, Bahceci, M., additional, Bargallo, M. F., additional, Arevalo, M. R., additional, Salat, M. M., additional, Barbat, I. V., additional, Lopez, J. T., additional, Algam, M. E., additional, Boluda, A. B., additional, de Oya, G. C., additional, Tolmacheva, E. N., additional, Kashevarova, A. A., additional, Skryabin, N. A., additional, Lebedev, I. N., additional, Semaco, E., additional, Belo, A., additional, Riboldi, M., additional, Luz, L., additional, Nobrega, N., additional, Mazetto, R., additional, Alegretti, J. A., additional, Bibancos, M., additional, Serafini, P., additional, Neupane, J., additional, Vandewoestyne, M., additional, Heindryckx, B., additional, Deroo, T., additional, Lu, Y., additional, Ghimire, S., additional, Lierman, S., additional, Qian, C., additional, Deforce, D., additional, De Sutter, P., additional, Viloria, T., additional, Martinez-Jabaloyas, J. M., additional, Gil-Salom, M., additional, Capalbo, A., additional, Treff, N., additional, Cimadomo, D., additional, Tao, X., additional, Ferry, K., additional, Ubaldi, F. M., additional, Rienzi, L., additional, Scott, R. T., additional, Katzorke, N., additional, Vogt, H. P., additional, Hehr, A., additional, Gassner, C., additional, Paulmann, B., additional, Kowalzyk, Z., additional, Klatt, M., additional, Krauss, S., additional, Seifert, D., additional, Seifert, B., additional, Hehr, U., additional, Lobascio, M., additional, Varricchio, M. T., additional, Rubino, P., additional, Bono, S., additional, Cotarelo, R. P., additional, Spizzichino, L., additional, Colicchia, A., additional, Giannini, P., additional, Suhorutshenko, M., additional, and Rosenstein-Tamm, K., additional
- Published
- 2013
- Full Text
- View/download PDF
4. Embryology
- Author
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Gandhi, G., primary, Allahbadia, G., additional, Kagalwala, S., additional, Allahbadia, A., additional, Ramesh, S., additional, Patel, K., additional, Hinduja, R., additional, Chipkar, V., additional, Madne, M., additional, Ramani, R., additional, Joo, J. K., additional, Jeung, J. E., additional, Go, K. R., additional, Lee, K. S., additional, Goto, H., additional, Hashimoto, S., additional, Amo, A., additional, Yamochi, T., additional, Iwata, H., additional, Morimoto, Y., additional, Koifman, M., additional, Lahav-Baratz, S., additional, Blais, E., additional, Megnazi-Wiener, Z., additional, Ishai, D., additional, Auslender, R., additional, Dirnfeld, M., additional, Zaletova, V., additional, Zakharova, E., additional, Krivokharchenko, I., additional, Zaletov, S., additional, Zhu, L., additional, Li, Y., additional, Zhang, H., additional, Ai, J., additional, Jin, L., additional, Zhang, X., additional, Rajan, N., additional, Kovacs, A., additional, Foley, C., additional, Flanagan, J., additional, O'Callaghan, J., additional, Waterstone, J., additional, Dineen, T., additional, Dahdouh, E. M., additional, St-Michel, P., additional, Granger, L., additional, Carranza-Mamane, B., additional, Faruqi, F., additional, Kattygnarath, T. V., additional, Gomes, F. L. A. F., additional, Christoforidis, N., additional, Ioakimidou, C., additional, Papas, C., additional, Moisidou, M., additional, Chatziparasidou, A., additional, Klaver, M., additional, Tilleman, K., additional, De Sutter, P., additional, Lammers, J., additional, Freour, T., additional, Splingart, C., additional, Barriere, P., additional, Ikeno, T., additional, Nakajyo, Y., additional, Sato, Y., additional, Hirata, K., additional, Kyoya, T., additional, Kyono, K., additional, Campos, F. B., additional, Meseguer, M., additional, Nogales, M., additional, Martinez, E., additional, Ariza, M., additional, Agudo, D., additional, Rodrigo, L., additional, Garcia-Velasco, J. A., additional, Lopes, A. S., additional, Frederickx, V., additional, Vankerkhoven, G., additional, Serneels, A., additional, Roziers, P., additional, Puttermans, P., additional, Campo, R., additional, Gordts, S., additional, Fragouli, E., additional, Alfarawati, S., additional, Spath, K., additional, Wells, D., additional, Liss, J., additional, Lukaszuk, K., additional, Glowacka, J., additional, Bruszczynska, A., additional, Gallego, S. C., additional, Lopez, L. O., additional, Vila, E. O., additional, Garcia, M. G., additional, Canas, C. L., additional, Segovia, A. G., additional, Ponce, A. G., additional, Calonge, R. N., additional, Peregrin, P. C., additional, Ito, K., additional, Nakaoka, Y., additional, Alcoba, D. D., additional, Valerio, E. G., additional, Conzatti, M., additional, Tornquist, J., additional, Kussler, A. P., additional, Pimentel, A. M., additional, Corleta, H. E., additional, Brum, I. S., additional, Boyer, P., additional, Montjean, D., additional, Tourame, P., additional, Gervoise-Boyer, M., additional, Cohen, J., additional, Lefevre, B., additional, Radio, C. I., additional, Wolf, J. P., additional, Ziyyat, A., additional, De Croo, I., additional, Tolpe, A., additional, Degheselle, S., additional, Van de Velde, A., additional, Van den Abbeel, E., additional, Gandhi, G., additional, Kuwayama, M., additional, Khatoon, A., additional, Alsule, S., additional, Inaba, M., additional, Ohgaki, A., additional, Ohtani, A., additional, Matsumoto, H., additional, Mizuno, S., additional, Mori, R., additional, Fukuda, A., additional, Umekawa, Y., additional, Yoshida, A., additional, Tanigiwa, S., additional, Seida, K., additional, Suzuki, H., additional, Tanaka, M., additional, Vahabi, Z., additional, Yazdi, P. E., additional, Dalman, A., additional, Ebrahimi, B., additional, Mostafaei, F., additional, Niknam, M. R., additional, Watanabe, S., additional, Kamihata, M., additional, Tanaka, T., additional, Matsunaga, R., additional, Yamanaka, N., additional, Kani, C., additional, Ishikawa, T., additional, Wada, T., additional, Morita, H., additional, Miyamura, H., additional, Nishio, E., additional, Ito, M., additional, Kuwahata, A., additional, Ochi, M., additional, Horiuchi, T., additional, Dal Canto, M., additional, Guglielmo, M. C., additional, Fadini, R., additional, Renzini, M. M., additional, Albertini, D. F., additional, Novara, P., additional, Lain, M., additional, Brambillasca, F., additional, Turchi, D., additional, Sottocornola, M., additional, Coticchio, G., additional, Kato, M., additional, Fukunaga, N., additional, Nagai, R., additional, Kitasaka, H., additional, Yoshimura, T., additional, Tamura, F., additional, Hasegawa, N., additional, Nakayama, K., additional, Takeuchi, M., additional, Ohno, H., additional, Aoyagi, N., additional, Kojima, E., additional, Itoi, F., additional, Hashiba, Y., additional, Asada, Y., additional, Kikuchi, H., additional, Iwasa, Y., additional, Kamono, T., additional, Suzuki, A., additional, Yamada, K., additional, Kanno, H., additional, Sasaki, K., additional, Murakawa, H., additional, Matsubara, M., additional, Yoshida, H., additional, Valdespin, C., additional, Elhelaly, M., additional, Chen, P., additional, Pangestu, M., additional, Catt, S., additional, Hojnik, N., additional, Kovacic, B., additional, Roglic, P., additional, Taborin, M., additional, Zafosnik, M., additional, Knez, J., additional, Vlaisavljevic, V., additional, Mori, C., additional, Yabuuchi, A., additional, Ezoe, K., additional, Takayama, Y., additional, Aono, F., additional, Kato, K., additional, Radwan, P., additional, Krasinski, R., additional, Chorobik, K., additional, Radwan, M., additional, Stoppa, M., additional, Maggiulli, R., additional, Capalbo, A., additional, Ievoli, E., additional, Dovere, L., additional, Scarica, C., additional, Albricci, L., additional, Romano, S., additional, Sanges, F., additional, Barnocchi, N., additional, Papini, L., additional, Vivarelli, A., additional, Ubaldi, F. M., additional, Rienzi, L., additional, Bono, S., additional, Spizzichino, L., additional, Rubio, C., additional, Fiorentino, F., additional, Ferris, J., additional, Favetta, L. A., additional, MacLusky, N., additional, King, W. A., additional, Madani, T., additional, Jahangiri, N., additional, Aflatoonian, R., additional, Cater, E., additional, Hulme, D., additional, Berrisford, K., additional, Jenner, L., additional, Campbell, A., additional, Fishel, S., additional, Zhang, X. Y., additional, Yilmaz, A., additional, Hananel, H., additional, Ao, A., additional, Vutyavanich, T., additional, Piromlertamorn, W., additional, Saenganan, U., additional, Samchimchom, S., additional, Wirleitner, B., additional, Lejeune, B., additional, Zech, N. H., additional, Vanderzwalmen, P., additional, Albani, E., additional, Parini, V., additional, Smeraldi, A., additional, Menduni, F., additional, Antonacci, R., additional, Marras, A., additional, Levi, S., additional, Morreale, G., additional, Pisano, B., additional, Di Biase, A., additional, Di Rosa, A., additional, Setti, P. E. L., additional, Puard, V., additional, Cadoret, V., additional, Tranchant, T., additional, Gauthier, C., additional, Reiter, E., additional, Guerif, F., additional, Royere, D., additional, Yoon, S. Y., additional, Eum, J. H., additional, Park, E. A., additional, Kim, T. Y., additional, Yoon, T. K., additional, Lee, D. R., additional, Lee, W. S., additional, Cabal, A. C., additional, Vallejo, B., additional, Campos, P., additional, Sanchez, E., additional, Serrano, J., additional, Remohi, J., additional, Nagornyy, V., additional, Mazur, P., additional, Mykytenko, D., additional, Semeniuk, L., additional, Zukin, V., additional, Guilherme, P., additional, Madaschi, C., additional, Bonetti, T. C. S., additional, Fassolas, G., additional, Izzo, C. R., additional, Santos, M. J. D. L., additional, Beltran, D., additional, Garcia-Laez, V., additional, Escriba, M. J., additional, Grau, N., additional, Escrich, L., additional, Albert, C., additional, Zuzuarregui, J. L., additional, Pellicer, A., additional, LU, Y., additional, Nikiforaki, D., additional, Meerschaut, F. V., additional, Neupane, J., additional, De Vos, W. H., additional, Lierman, S., additional, Deroo, T., additional, Heindryckx, B., additional, Li, J., additional, Chen, X. Y., additional, Lin, G., additional, Huang, G. N., additional, Sun, Z. Y., additional, Zhong, Y., additional, Zhang, B., additional, Li, T., additional, Zhang, S. P., additional, Ye, H., additional, Han, S. B., additional, Liu, S. Y., additional, Zhou, J., additional, Lu, G. X., additional, Zhuang, G. L., additional, Muela, L., additional, Roldan, M., additional, Gadea, B., additional, Martinez, M., additional, Perez, I., additional, Munoz, M., additional, Castello, C., additional, Asensio, M., additional, Fernandez, P., additional, Farreras, A., additional, Rovira, S., additional, Capdevila, J. M., additional, Velilla, E., additional, Lopez-Teijon, M., additional, Kovacs, P., additional, Matyas, S. Z., additional, Forgacs, V., additional, Reichart, A., additional, Rarosi, F., additional, Bernard, A., additional, Torok, A., additional, Kaali, S. G., additional, Sajgo, A., additional, Pribenszky, C. S., additional, Sozen, B., additional, Ozturk, S., additional, Yaba-Ucar, A., additional, Demir, N., additional, Gelo, N., additional, Stanic, P., additional, Hlavati, V., additional, ogoric, S., additional, Pavicic-Baldani, D., additional, prem-Goldtajn, M., additional, Radakovic, B., additional, Kasum, M., additional, Strelec, M., additional, Canic, T., additional, imunic, V., additional, Vrcic, H., additional, Ajina, M., additional, Negra, D., additional, Ben-Ali, H., additional, Jallad, S., additional, Zidi, I., additional, Meddeb, S., additional, Bibi, M., additional, Khairi, H., additional, Saad, A., additional, Gamiz, P., additional, Viloria, T., additional, Lima, E. T., additional, Fernandez, M. P., additional, Prieto, J. A. A., additional, Varela, M. O., additional, Kassa, D., additional, Munoz, E. M., additional, Kani, K., additional, Nor-Ashikin, M. N. K., additional, Norhazlin, J. M. Y., additional, Norita, S., additional, Wan-Hafizah, W. J., additional, Mohd-Fazirul, M., additional, Razif, D., additional, Hoh, B. P., additional, Dale, S., additional, Woodhead, G., additional, Andronikou, S., additional, Francis, G., additional, Tailor, S., additional, Vourliotis, M., additional, Almeida, P. A., additional, Krivega, M., additional, Van de Velde, H., additional, Lee, R. K., additional, Hwu, Y. M., additional, Lu, C. H., additional, Li, S. H., additional, Vaiarelli, A., additional, Desgro, M., additional, Baggiani, A., additional, Zannoni, E., additional, Kermavner, L. B., additional, Klun, I. V., additional, Pinter, B., additional, Vrtacnik-Bokal, E., additional, De Paepe, C., additional, Cauffman, G., additional, Verheyen, G., additional, Stoop, D., additional, Liebaers, I., additional, Stecher, A., additional, Zintz, M., additional, Neyer, A., additional, Bach, M., additional, Baramsai, B., additional, Schwerda, D., additional, Wiener-Megnazi, Z., additional, Fridman, M., additional, Blais, I., additional, Akerud, H., additional, Lindgren, K., additional, Karehed, K., additional, Wanggren, K., additional, Hreinsson, J., additional, Freijomil, B., additional, Weiss, A., additional, Neril, R., additional, Geslevich, J., additional, Beck-Fruchter, R., additional, Lavee, M., additional, Golan, J., additional, Ermoshkin, A., additional, Shalev, E., additional, Shi, W., additional, Zhang, S., additional, Zhao, W., additional, Xue, X. I. A., additional, Wang, M. I. N., additional, Bai, H., additional, Shi, J., additional, Smith, H. L., additional, Shaw, L., additional, Kimber, S., additional, Brison, D., additional, Boumela, I., additional, Assou, S., additional, Haouzi, D., additional, Ahmed, O. A., additional, Dechaud, H., additional, Hamamah, S., additional, Dasiman, R., additional, Nor-Shahida, A. R., additional, Salina, O., additional, Gabriele, R. A. F., additional, Ben-Yosef, D., additional, Shwartz, T., additional, Cohen, T., additional, Carmon, A., additional, Raz, N. M., additional, Malcov, M., additional, Frumkin, T., additional, Almog, B., additional, Vagman, I., additional, Kapustiansky, R., additional, Reches, A., additional, Azem, F., additional, Amit, A., additional, Cetinkaya, M., additional, Pirkevi, C., additional, Yelke, H., additional, Kumtepe, Y., additional, Atayurt, Z., additional, Kahraman, S., additional, Risco, R., additional, Hebles, M., additional, Saa, A. M., additional, Vilches-Ferron, M. A., additional, Sanchez-Martin, P., additional, Lucena, E., additional, Lucena, M., additional, Heras, M. D. L., additional, Agirregoikoa, J. A., additional, Barrenetxea, G., additional, De Pablo, J. L., additional, Lehner, A., additional, Pribenszky, C., additional, Murber, A., additional, Rigo, J., additional, Urbancsek, J., additional, Fancsovits, P., additional, Bano, D. G., additional, Sanchez-Leon, A., additional, Marcos, J., additional, Molla, M., additional, Amorocho, B., additional, Nicolas, M., additional, Fernandez, L., additional, Landeras, J., additional, Adeniyi, O. A., additional, Ehbish, S. M., additional, Brison, D. R., additional, Egashira, A., additional, Murakami, M., additional, Nagafuchi, E., additional, Tanaka, K., additional, Tomohara, A., additional, Mine, C., additional, Otsubo, H., additional, Nakashima, A., additional, Otsuka, M., additional, Yoshioka, N., additional, Kuramoto, T., additional, Choi, D., additional, Yang, H., additional, Park, J. H., additional, Jung, J. H., additional, Hwang, H. G., additional, Lee, J. H., additional, Lee, J. E., additional, Kang, A. S., additional, Yoo, J. H., additional, Kwon, H. C., additional, Lee, S. J., additional, Bang, S., additional, Shin, H., additional, Lim, H. J., additional, Min, S. H., additional, Yeon, J. Y., additional, Koo, D. B., additional, Higo, S., additional, Ruvalcaba, L., additional, Kobayashi, M., additional, Takeuchi, T., additional, Miwa, A., additional, Nagai, Y., additional, Momma, Y., additional, Takahashi, K., additional, Chuko, M., additional, Nagai, A., additional, Otsuki, J., additional, Kim, S. G., additional, Kim, Y. Y., additional, Kim, H. J., additional, Park, I. H., additional, Sun, H. G., additional, Lee, K. H., additional, Song, H. J., additional, Costa-Borges, N., additional, Belles, M., additional, Herreros, J., additional, Teruel, J., additional, Ballesteros, A., additional, Calderon, G., additional, Vossaert, L., additional, Qian, C., additional, Lu, Y., additional, Parys, J. B., additional, Deforce, D., additional, Leybaert, L., additional, Surlan, L., additional, Otasevic, V., additional, Velickovic, K., additional, Golic, I., additional, Vucetic, M., additional, Stankovic, V., additional, Stojnic, J., additional, Radunovic, N., additional, Tulic, I., additional, Korac, B., additional, Korac, A., additional, Elias, R., additional, Neri, Q. V., additional, Fields, T., additional, Schlegel, P. N., additional, Rosenwaks, Z., additional, Palermo, G. D., additional, Gilson, A., additional, Piront, N., additional, Heens, B., additional, Vastersaegher, C., additional, Vansteenbrugge, A., additional, Pauwels, P. C. P., additional, Abdel-Raheem, M. F., additional, Abdel-Rahman, M. Y., additional, Abdel-Gaffar, H. M., additional, Sabry, M., additional, Kasem, H., additional, Rasheed, S. M., additional, Amin, M., additional, Abdelmonem, A., additional, Ait-Allah, A. S., additional, VerMilyea, M., additional, Anthony, J., additional, Bucci, J., additional, Croly, S., additional, Coutifaris, C., additional, Cimadomo, D., additional, Dusi, L., additional, Colamaria, S., additional, Baroni, E., additional, Giuliani, M., additional, Sapienza, F., additional, Buffo, L., additional, Zivi, E., additional, Aizenman, E., additional, Barash, D., additional, Gibson, D., additional, Shufaro, Y., additional, Perez, M., additional, Aguilar, J., additional, Taboas, E., additional, Ojeda, M., additional, Suarez, L., additional, Munoz, E., additional, Casciani, V., additional, Minasi, M. G., additional, Scarselli, F., additional, Terribile, M., additional, Zavaglia, D., additional, Colasante, A., additional, Franco, G., additional, Greco, E., additional, Hickman, C., additional, Cook, C., additional, Gwinnett, D., additional, Trew, G., additional, Carby, A., additional, Lavery, S., additional, Asgari, L., additional, Paouneskou, D., additional, Jayaprakasan, K., additional, Maalouf, W., additional, Campbell, B. K., additional, Rega, E., additional, Alteri, A., additional, Cotarelo, R. P., additional, Rubino, P., additional, Colicchia, A., additional, Giannini, P., additional, Devjak, R., additional, Papler, T. B., additional, Tacer, K. F., additional, Verdenik, I., additional, Iussig, B., additional, Gala, A., additional, Ferrieres, A., additional, Vincens, C., additional, Bringer-Deutsch, S., additional, Brunet, C., additional, Conaghan, J., additional, Tan, L., additional, Gvakharia, M., additional, Ivani, K., additional, Chen, A., additional, Pera, R. R., additional, Bowman, N., additional, Montgomery, S., additional, Best, L., additional, Duffy, S., additional, Hirata, R., additional, Aoi, Y., additional, Habara, T., additional, Hayashi, N., additional, Dinopoulou, V., additional, Partsinevelos, G. A., additional, Bletsa, R., additional, Mavrogianni, D., additional, Anagnostou, E., additional, Stefanidis, K., additional, Drakakis, P., additional, Loutradis, D., additional, Hernandez, J., additional, Leon, C. L., additional, Puopolo, M., additional, Palumbo, A., additional, Atig, F., additional, Kerkeni, A., additional, D'Ommar, G., additional, Herrera, A. K., additional, Lozano, L., additional, Majerfeld, M., additional, Ye, Z., additional, Zaninovic, N., additional, Clarke, R., additional, Bodine, R., additional, Nagorny, V., additional, Zabala, A., additional, Pessino, T., additional, Outeda, S., additional, Blanco, L., additional, Leocata, F., additional, Asch, R., additional, Rajikin, M. H., additional, Nuraliza, A. S., additional, Machac, S., additional, Hubinka, V., additional, Larman, M., additional, Koudelka, M., additional, Budak, T. P., additional, Membrado, O. O., additional, Martinez, E. S., additional, Wilson, P., additional, McClure, A., additional, Nargund, G., additional, Raso, D., additional, Insua, M. F., additional, Lotti, B., additional, Giordana, S., additional, Baldi, C., additional, Barattini, J., additional, Cogorno, M., additional, Peri, N. F., additional, Neuspiller, F., additional, Resta, S., additional, Filannino, A., additional, Maggi, E., additional, Cafueri, G., additional, Ferraretti, A. P., additional, Magli, M. C., additional, Gianaroli, L., additional, Sioga, A., additional, Oikonomou, Z., additional, Chatzimeletiou, K., additional, Oikonomou, L., additional, Kolibianakis, E., additional, Tarlatzis, B. C., additional, Sarkar, M. R., additional, Ray, D., additional, Bhattacharya, J., additional, Alises, J. M., additional, Gumbao, D., additional, Hickman, C. F. L., additional, Fiorentino, I., additional, Gualtieri, R., additional, Barbato, V., additional, Braun, S., additional, Mollo, V., additional, Netti, P., additional, Talevi, R., additional, Bayram, A., additional, Findikli, N., additional, Serdarogullari, M., additional, Sahin, O., additional, Ulug, U., additional, Tosun, S. B., additional, Bahceci, M., additional, Leon, A. S., additional, Cardoso, M. C. A., additional, Aguiar, A. P. S., additional, Sartorio, C., additional, Evangelista, A., additional, Gallo-Sa, P., additional, Erthal-Martins, M. C., additional, Mantikou, E., additional, Jonker, M. J., additional, de Jong, M., additional, Wong, K. M., additional, van Montfoort, A. P. A., additional, Breit, T. M., additional, Repping, S., additional, Mastenbroek, S., additional, Power, E., additional, Jordan, K., additional, Aksoy, T., additional, Gultomruk, M., additional, Aktan, A., additional, Goktas, C., additional, Petracco, R., additional, Okada, L., additional, Azambuja, R., additional, Badalotti, F., additional, Michelon, J., additional, Reig, V., additional, Kvitko, D., additional, Tagliani-Ribeiro, A., additional, Badalotti, M., additional, Petracco, A., additional, Aydin, B., additional, Cepni, I., additional, Rodriguez-Arnedo, D., additional, Ten, J., additional, Guerrero, J., additional, Ochando, I., additional, and Bernabeu, R., additional
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- 2013
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5. Promuclear transfer in mice yields minimal mitochondrial DNA carry-over
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Vandewoestyne, M., primary, Neupane, J., additional, Heindryckx, B., additional, Lierman, S., additional, Deforce, D., additional, and De Sutter, P., additional
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- 2012
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6. STEM CELLS
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Altomare, R., primary, Marino, A., additional, Curcio, P., additional, Volpes, A., additional, Santoro, A., additional, Lo Monte, A. I., additional, Mazzola, S., additional, Allegra, A., additional, Ghimire, S., additional, Van der Jeught, M., additional, Neupane, J., additional, Lierman, S., additional, O'Leary, T., additional, Chuva de Sousa Lopes, S., additional, Heindryckx, B., additional, De Sutter, P., additional, Sudoma, I., additional, Pylyp, L., additional, Goncharova, Y., additional, Zukin, V., additional, Duggal, G., additional, Deforce, D., additional, Cakici, C., additional, Buyrukcu, B., additional, Aksoy, A., additional, Haliloglu, A., additional, Duruksu, G., additional, Uludag, O., additional, Isik, A., additional, Subasi, C., additional, and Karaoz, E., additional
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- 2012
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7. Audit of Clinical Care Received by COVID-19 Patients Treated at a Tertiary Care Hospital of Nepal in 2021.
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Mandal SK, Neupane J, Kumar AMV, Davtyan H, Thekkur P, Jayaram A, Chalise BS, Rawal M, Paudel M, Baral B, Shah RK, Maharjan K, Shrestha S, Bhandari L, K C N, Gautam N, Sunny AK, Thakur N, Subeedee KC, Mandal SK, and Bastola A
- Abstract
Like the world over, Nepal was also hard hit by the second wave of COVID-19. We audited the clinical care provided to COVID-19 patients admitted from April to June 2021 in a tertiary care hospital of Nepal. This was a cohort study using routinely collected hospital data. There were 620 patients, and most (458, 74%) had severe illness. The majority (600, 97%) of the patients were eligible for admission as per national guidelines. Laboratory tests helping to predict the outcome of COVID-19, such as D-dimer and C-reactive protein, were missing in about 25% of patients. Nearly all (>95%) patients with severe disease received corticosteroids, anticoagulants and oxygen. The use of remdesivir was low (22%). About 70% of the patients received antibiotics. Hospital exit outcomes of most (>95%) patients with mild and moderate illness were favorable (alive and discharged). Among patients with severe illness, about 25% died and 4% were critically ill, needing further referral. This is the first study from Nepal to audit and document COVID-19 clinical care provision in a tertiary care hospital, thus filling the evidence gap in this area from resource-limited settings. Adherence to admission guidelines was excellent. Laboratory testing, access to essential drugs and data management needs to be improved.
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- 2022
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8. Effects of irrigation rates on cotton yield as affected by soil physical properties and topography in the southern high plains.
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Neupane J, Guo W, West CP, Zhang F, and Lin Z
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- Texas, Crop Production methods, Farms, Agriculture methods, Agricultural Irrigation methods, Gossypium growth & development, Soil chemistry
- Abstract
Lack of precipitation and groundwater for irrigation limits crop production in semi-arid regions, such as the Southern High Plains (SHP). Advanced technologies, such as variable rate irrigation (VRI), can conserve water and improve water use efficiency for sustainable agriculture. However, the adoption of VRI is hindered by the lack of on-farm research focusing on the feasibility of VRI. The objective of this study was to assess the effect of irrigation rates on cotton yield as affected by soil physical properties and topography in the Southern High Plains. This study was conducted in two fields within a 194-ha commercially managed farm in Hale County, Texas, in 2017. An irrigation treatment with three rates was implemented in a randomized complete block design with two replications as separate blocks in each field. A total of 230 composite soil samples were collected from the farm in spring 2017 and analyzed for texture. Information on apparent soil electrical conductivity (ECa), elevation, and final yield were collected from the fields. A statistical model showed that the effect of irrigation rates on cotton yield depended on its interaction with soil physical properties and topography. For example, areas with slope >2% and sand content >50% had no significant response to higher irrigation rates. This model suggests that applying irrigation amounts based on the yield response can be a basis for VRI. This study provides valuable information for site-specific irrigation to optimize crop production in fields with significant variability in soil physical properties and topography., Competing Interests: This does not alter our adherence to PLOS ONE policies on sharing data and materials. Cotton Incorporated is a not-for-profit organization that supports and funds research projects, including this study (https://www.cottoninc.com/).
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- 2021
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9. Persistent Symptoms in Post-COVID-19 Patients Attending Follow-Up OPD at Sukraraj Tropical and Infectious Disease Hospital (STIDH), Kathmandu, Nepal.
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Bastola A, Nepal R, Shrestha B, Maharjan K, Shrestha S, Chalise BS, and Neupane J
- Abstract
The long-term effects of COVID-19 among survivors is a matter of concern. This research aimed to study persistent symptoms in post-COVID-19 patients attending a follow-up clinic at a tertiary care hospital in Nepal. All patients, presenting to the outpatient clinic during the study duration of six weeks, with history of positive reverse transcriptase- polymerase chain reaction for severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) at least two weeks prior to presentation, were included. The duration of follow-up ranged from 15 till 150 days with the mean duration of 28 days after diagnosis of COVID-19. Of 118 patients, 43 (36.4%) had a history of mild COVID-19, 15 (12.8%) had moderate, and 60 (50.8%) had severe. At the time of presentation, 97 (82.2%) patients reported that they had at least one persistent/new symptom beyond two weeks from the diagnosis of COVID-19. Dyspnea, fatigue, chest heaviness, and cough were the commonest persistent complaints in 48 (40.7%), 39 (33.1%), 33 (28%), and 32 (27.1%) patients, respectively. The findings in our study highlight the need for extended monitoring of post-COVID-19 patients following discharge, in order to understand and mitigate long-term implications of the disease.
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- 2021
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10. The unfolding body plan of primate embryos in culture.
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Neupane J, Wong FCK, and Surani MA
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- Animals, Primates, Embryo Culture Techniques, Embryonic Development
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- 2020
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11. Characterization of Leptazolines A-D, Polar Oxazolines from the Cyanobacterium Leptolyngbya sp., Reveals a Glitch with the "Willoughby-Hoye" Scripts for Calculating NMR Chemical Shifts.
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Bhandari Neupane J, Neupane RP, Luo Y, Yoshida WY, Sun R, and Williams PG
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- Magnetic Resonance Spectroscopy standards, Molecular Structure, Oxazoles chemistry, Reference Standards, Stereoisomerism, Cyanobacteria chemistry, Oxazoles chemical synthesis
- Abstract
The bioactivity-guided examination of a Leptolyngbya sp. led to the isolation of leptazolines A-D ( 1 - 4 ), from the culture media, along with two degradation products ( 5 and 6 ). Density functional theory nuclear magnetic resonance calculations established the relative configurations of 1 and 2 and revealed that the calculated shifts depended on the operating system when using the "Willoughby-Hoye" Python scripts to streamline the processing of the output files, a previously unrecognized flaw that could lead to incorrect conclusions.
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- 2019
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12. Culture conditions affect Ca 2+ release in artificially activated mouse and human oocytes.
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Lu Y, Bonte D, Ferrer-Buitrago M, Popovic M, Neupane J, Van der Jeught M, Leybaert L, De Sutter P, and Heindryckx B
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- Adult, Animals, Calcium Ionophores pharmacology, Culture Media, Embryonic Development drug effects, Female, Humans, Ionomycin pharmacology, Mice, Oocytes drug effects, Oocytes metabolism, Young Adult, Calcium metabolism, Embryo Culture Techniques, Embryonic Development physiology, Oocytes cytology
- Abstract
Inconsistent fertilisation and pregnancy rates have been reported by different laboratories after application of ionomycin as a clinical method of assisted oocyte activation (AOA) to overcome fertilisation failure. Using both mouse and human oocytes, in the present study we investigated the effects of ionomycin and Ca2+ concentrations on the pattern of Ca2+ release and embryonic developmental potential. In the mouse, application of 5μM ionomycin in potassium simplex optimisation medium (KSOM) or 10µM ionomycin in Ca2+-free KSOM significantly reduced the Ca2+ flux and resulted in failure of blastocyst formation compared with 10μM ionomycin in KSOM. Increasing the Ca2+ concentration up to three- or sixfold did not benefit mouse embryonic developmental potential. Similarly, 10μM ionomycin-induced rise in Ca2+ in human oocytes increased with increasing total calcium concentrations in the commercial medium. Remarkably, we observed significantly reduced mouse embryo development when performing AOA over a period of 10min in Quinn's AdvantageTM Fertilisation medium (Cooper Surgical) and IVFTM medium (Vitrolife) compared with Sydney IVF COOK cleavage medium (Cook Ireland), using the same sequential culture system from the post-activation stage to blastocyst formation stage in different AOA groups. In conclusion, concentrations of both ionomycin and Ca2+ in culture media used during AOA can have significant effects on Ca2+ release and further embryonic developmental potential.
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- 2018
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13. Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background.
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Ghimire S, Van der Jeught M, Neupane J, Roost MS, Anckaert J, Popovic M, Van Nieuwerburgh F, Mestdagh P, Vandesompele J, Deforce D, Menten B, Chuva de Sousa Lopes S, De Sutter P, and Heindryckx B
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- Animals, Cell Differentiation, Embryo, Mammalian, Fibroblast Growth Factor 5 genetics, Fibroblast Growth Factor 5 metabolism, GATA6 Transcription Factor genetics, GATA6 Transcription Factor metabolism, Gene Ontology, Genetic Background, HMGB Proteins genetics, HMGB Proteins metabolism, Keratin-18 genetics, Keratin-18 metabolism, Mice, Molecular Sequence Annotation, Mouse Embryonic Stem Cells cytology, Nanog Homeobox Protein genetics, Nanog Homeobox Protein metabolism, Octamer Transcription Factor-3 genetics, Octamer Transcription Factor-3 metabolism, Pluripotent Stem Cells cytology, SOXB1 Transcription Factors genetics, SOXB1 Transcription Factors metabolism, SOXF Transcription Factors genetics, SOXF Transcription Factors metabolism, Signal Transduction, Gene Expression Regulation, Developmental, Mouse Embryonic Stem Cells metabolism, Pluripotent Stem Cells metabolism, Transcriptome
- Abstract
Mouse embryonic stem cells (mESCs) exist in a naive, primed and ground state of pluripotency. While comparative analyses of these pluripotency states have been reported, the mESCs utilized originated from various genetic backgrounds and were derived in different laboratories. mESC derivation in conventional LIF + serum culture conditions is strain dependent, with different genetic backgrounds potentially affecting subsequent stem cell characteristics. In the present study, we performed a comprehensive characterization of naive, primed and ground state mESCs originating from the same genetic background within our laboratory, by comparing their transcriptional profiles. We showed unique transcriptional profiles for naive, primed and ground state mESCs. While naive and ground state mESCs have more similar but not identical profiles, primed state mESCs show a very distinct profile. We further demonstrate that the differentiation propensity of mESCs to specific germ layers is highly dependent on their respective state of pluripotency.
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- 2018
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14. Strontium fails to induce Ca 2+ release and activation in human oocytes despite the presence of functional TRPV3 channels.
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Lu Y, Reddy R, Ferrer Buitrago M, Vander Jeught M, Neupane J, De Vos WH, Van den Abbeel E, Lierman S, De Sutter P, and Heindryckx B
- Abstract
Study Question: Are the transient receptor potential cation channels vanilloid 3 (TRPV3) present and able to mediate strontium (Sr
2+ ) induced artificial activation in human oocytes?, Summary Answer: Sr2+ did not induce Ca2+ rises or provoke activation in human oocytes, however, mRNA for the TRPV3 channel was present in metaphase II (MII) human oocytes after IVM and TRPV3 agonists induced Ca2+ rises and oocyte activation, demonstrating the channels were functional., What Is Known Already: Selective activation of TRPV3 by agonists induces Ca2+ entry and promotes mouse oocyte activation, and the absence of TRPV3 channels in mouse oocytes prevents Sr2+ mediated artificial activation. Sr2+ is sometimes used to overcome fertilization failure after ICSI in the clinic, but its efficiency is still controversial and the mechanism(s) of how it mediates the Ca2+ flux has not been studied yet in human., Study Design Size Duration: The protein distribution ( n = 10) and mRNA expression level ( n = 19) of the TRPV3 channels was investigated in human MII oocytes after IVM. The Sr2+ (10 mM) and TRPV3 agonists (200 μM 2-aminoethoxydiphenyl borate [2-APB] and 200 μM carvacrol)-induced Ca2+ response was analyzed in human ( n = 15, n = 16 and n = 16, respectively) and mouse oocytes ( n = 15, n = 19 and n = 26, respectively). The subsequent embryonic developmental potential following the parthenogenetic activation using these three agents was recorded in human ( n = 10, n = 9 and n = 9, respectively) and mouse ( n = 20 per agent) oocytes, by determining pronucleus, or 2-cell and blastocyst formation rates., Participants/materials Setting Methods: MII oocytes from B6D2F1 mice (6-10 weeks old) as well as human IVM oocytes and IVO oocytes (from patients aged 25-38 years old) with aggregates of smooth endoplasmic reticulum clusters were used. The expression of TRPV3 channels was determined by immunofluorescence staining with confocal microscopy and RT-PCR, and the temporal evolution of intracellular Ca2+ concentration was measured by time-lapse imaging after exposure to Sr2+ and TRPV3 agonists (2-APB and carvacrol). Artificial activation efficiency was assessed using these agents., Main Results and the Role of Chance: Sr2+ did not promote Ca2+ oscillations or provoke activation in human oocytes. Transcripts of TRPV3 channels were present in IVM MII human oocytes. TRPV3 protein was expressed and distributed throughout the ooplasm of human oocytes, rather than particularly concentrated in plasma membrane as observed in mouse MII oocytes. Both agonists of TRPV3 (2-APB and carvacrol), promoted a single Ca2+ transient and activated a comparable percentage of more than half of the exposed human oocytes ( P > 0.05). The agonist 2-APB was also efficient in activating mouse oocytes, however, significantly fewer mouse oocytes responded to carvacrol than 2-APB in both the Ca2+ analysis and activation test ( P < 0.001)., Limitations Reasons for Caution: The availability of fresh IVO matured oocytes in human was limited. Data from TRPV3 knockout model are not included., Wider Implications of the Findings: The benefit of clinical application using Sr2+ to overcome fertilization failure after ICSI requires further validation., Study Funding/competing Interests: This study was supported by FWO-Vlaanderen, China Scholarship Council and Special Research Fund from Ghent University (Bijzonder Onderzoeksfonds, BOF). No competing interests are declared.- Published
- 2018
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15. Patients with a high proportion of immature and meiotically resistant oocytes experience defective nuclear oocyte maturation patterns and impaired pregnancy outcomes.
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Lu Y, Ferrer-Buitrago M, Popovic M, Neupane J, De Vos WH, Lierman S, Van den Abbeel E, Van der Jeught M, Nikiforaki D, De Sutter P, and Heindryckx B
- Subjects
- Adult, Animals, Calcium metabolism, Female, Humans, Mice, Oocyte Retrieval, Oocytes metabolism, Pregnancy, Pregnancy Outcome, Treatment Failure, In Vitro Oocyte Maturation Techniques, Meiosis physiology, Oocytes cytology, Ovulation Induction
- Abstract
Patients presenting with abnormally high numbers of immature oocytes at retrieval are more likely to exhibit maturation resistant oocytes. However, the clinical relevance of such events remains unknown. We investigated nuclear maturation competence of immature oocytes from patients showing >40% of collected immature oocytes (Study group) and Controls, in which a normal number of mature oocytes (≥60%) was retrieved. Following in-vitro culture, oocytes were classified as maturation resistant or in-vitro matured (IVM). Treatment outcomes were evaluated in Study and Control groups based on presence of maturation resistant oocytes. Overall, similarly high spindle and chromosome abnormality rates were observed in maturation resistant oocytes from both Study and Control groups. IVM oocytes from the Study group revealed significantly higher percentages of misaligned chromosomes compared with Controls (P < 0.05). Remarkably, Study group patients with at least one maturation resistant oocyte showed significantly reduced cumulative pregnancy and live birth rates compared with Control group maturation resistant patients (P < 0.05). When further investigating the aetiology, a maturation resistant mouse model revealed defective Ca
2+ signalling of maturation resistant oocytes at germinal vesicular breakdown and parthenogenetic activation. In conclusion, appropriate treatment strategies, including clinical utilization of IVM oocytes from Study group patients, warrant further investigation., (Copyright © 2018. Published by Elsevier Ltd.)- Published
- 2018
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16. Cellular Heterogeneity in the Level of mtDNA Heteroplasmy in Mouse Embryonic Stem Cells.
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Neupane J, Ghimire S, Vandewoestyne M, Lu Y, Gerris J, Van Coster R, Deroo T, Deforce D, Vansteelandt S, De Sutter P, and Heindryckx B
- Subjects
- Animals, Cell Division, Cells, Cultured, Genetic Heterogeneity, Haplotypes, Mice, DNA, Mitochondrial genetics, Mouse Embryonic Stem Cells physiology
- Abstract
Variation in the level of mtDNA heteroplasmy in adult tissues is commonly seen in patients with a mixture of wild-type and mutant mtDNA. A mixture of different mtDNA variants may influence such variation and cause mtDNA segregation bias. We analyzed cellular heterogeneity in embryonic stem cells (ESCs) derived from a polymorphic mouse model containing NZB and BALB mtDNA genotypes. In ESCs, inter-colony heterogeneity varied up to 61%, whereas intra-colony heterogeneity varied up to 100%. Three out of five cell lines displayed nearly homoplasmic BALB and NZB mtDNA haplotypes in differentiated single cells. The proportion of NZB mtDNA genotype increased with progressive passaging (0.39%; p = 0.002). These results demonstrate the bimodal segregation of mtDNA haplotypes, indicating the occurrence of tissues with variable levels of heteroplasmies in individuals with mtDNA mutations. Furthermore, proliferation of one mtDNA genotype over another may pose the risk of accumulating mutant mtDNAs during subsequent cell divisions., (Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2015
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17. Inhibition of transforming growth factor β signaling promotes epiblast formation in mouse embryos.
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Ghimire S, Heindryckx B, Van der Jeught M, Neupane J, O'Leary T, Lierman S, De Vos WH, Chuva de Sousa Lopes S, Deroo T, and De Sutter P
- Subjects
- Activating Transcription Factor 3 metabolism, Animals, Bone Morphogenetic Protein 4 metabolism, Cell Proliferation, Cyclic AMP-Dependent Protein Kinases metabolism, Embryonic Stem Cells cytology, Embryonic Stem Cells metabolism, Embryonic Stem Cells physiology, Germ Layers embryology, Germ Layers metabolism, Glycogen Synthase Kinase 3 metabolism, Janus Kinases metabolism, Mice, Cell Lineage, Germ Layers cytology, Signal Transduction, Transforming Growth Factor beta metabolism
- Abstract
Early lineage segregation in preimplantation embryos and maintenance of pluripotency in embryonic stem cells (ESCs) are both regulated by specific signaling pathways. Small molecules have been shown to modulate these signaling pathways. We examined the influence of several small molecules and growth factors on second-lineage segregation of the inner cell mass toward hypoblast and epiblast lineage during mouse embryonic preimplantation development. We found that the second-lineage segregation is influenced by activation or inhibition of the transforming growth factor (TGF)β pathway. Inhibition of the TGFβ pathway from the two-cell, four-cell, and morula stages onward up to the blastocyst stage significantly increased the epiblast cell proliferation. The epiblast formed in the embryos in which TGFβ signaling was inhibited was fully functional as demonstrated by the potential of these epiblast cells to give rise to pluripotent ESCs. Conversely, activating the TGFβ pathway reduced epiblast formation. Inhibition of the glycogen synthase kinase (GSK)3 pathway and activation of bone morphogenetic protein 4 signaling reduced the formation of both epiblast and hypoblast cells. Activation of the protein kinase A pathway and of the Janus kinase/signal transducer and activator of transcription 3 pathway did not influence the second-lineage segregation in mouse embryos. The simultaneous inhibition of three pathways--TGFβ, GSK3β, and the fibroblast growth factor (FGF)/extracellular signal-regulated kinases (Erk)--significantly enhanced the proliferation of epiblast cells than that caused by inhibition of either TGFβ pathway alone or by combined inhibition of the GSK3β and FGF/Erk pathways only.
- Published
- 2015
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18. Assessment of nuclear transfer techniques to prevent the transmission of heritable mitochondrial disorders without compromising embryonic development competence in mice.
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Neupane J, Vandewoestyne M, Ghimire S, Lu Y, Qian C, Van Coster R, Gerris J, Deroo T, Deforce D, De Sutter P, and Heindryckx B
- Subjects
- Animals, Blastocyst physiology, Blastomeres chemistry, DNA, Mitochondrial analysis, Female, Male, Mice, Pregnancy, Embryonic Development, Mitochondrial Diseases prevention & control, Nuclear Transfer Techniques adverse effects
- Abstract
To evaluate and compare mitochondrial DNA (mtDNA) carry-over and embryonic development potential between different nuclear transfer techniques we performed germinal vesicle nuclear transfer (GV NT), metaphase-II spindle-chromosome-complex (MII-SCC) transfer and pronuclear transfer (PNT) in mice. No detectable mtDNA carry-over was seen in most of the reconstructed oocytes and embryos. No significant differences were seen in mtDNA carry-over rate between GV NT (n=20), MII-SCC transfer (0.29 ± 0.63; n=21) and PNT (0.29 ± 0.75; n=25). Blastocyst formation was not compromised after either PNT (88%; n=18) or MII-SCC transfer (86%; n=27). Further analysis of blastomeres from cleaving embryos (n=8) demonstrated undetectable mtDNA carry-over in all but one blastomere. We show that NT in the germ line is potent to prevent transmission of heritable mtDNA disorders with the applicability for patients attempting reproduction., (Copyright © 2014 Elsevier B.V. and Mitochondria Research Society. All rights reserved.)
- Published
- 2014
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19. Mutation-free baby born from a mitochondrial encephalopathy, lactic acidosis and stroke-like syndrome carrier after blastocyst trophectoderm preimplantation genetic diagnosis.
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Heindryckx B, Neupane J, Vandewoestyne M, Christodoulou C, Jackers Y, Gerris J, Van den Abbeel E, Van Coster R, Deforce D, and De Sutter P
- Subjects
- Adult, Biopsy, Embryo Transfer, Female, Humans, Infant, Newborn, Male, Pregnancy, MELAS Syndrome diagnosis, MELAS Syndrome prevention & control, Pregnancy Complications, Preimplantation Diagnosis
- Abstract
To investigate the applicability of preimplantation genetic diagnosis (PGD), we used trophectoderm (TE) biopsy to determine the mutation load in a 35-year-old female with mitochondrial encephalopathy, lactic acidosis and stroke-like syndrome (MELAS). Transfer of a mutation-free blastocyst gave birth to a healthy boy with undetectable mutation in any of the analyzed tissues. We found strong correlation among TE cells (r=0.90) within blastocysts and also between cytoplasmic fragments and TE (r=0.95). This is the first case of mutation-free baby born from a MELAS patient after TE biopsy and supports the applicability of blastocyst PGD for patients with mtDNA disorders to establish healthy offspring., (Copyright © 2014 Elsevier B.V. and Mitochondria Research Society. All rights reserved.)
- Published
- 2014
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20. Poor correlation between polar bodies and blastomere mutation load in a patient with m.3243A>G tRNALeu(UUR) point mutation.
- Author
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Vandewoestyne M, Heindryckx B, De Gheselle S, Lepez T, Neupane J, Gerris J, Van Coster R, De Sutter P, and Deforce D
- Subjects
- Humans, Blastomeres cytology, DNA, Mitochondrial genetics, Point Mutation, Polar Bodies cytology, RNA, Transfer, Leu genetics
- Published
- 2012
- Full Text
- View/download PDF
21. The incidence of thyroid carcinoma in multinodular goiter: prospective study.
- Author
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Pradhan GB, Shrestha R, Shrestha S, Neupane J, and Bhattachan CL
- Subjects
- Adult, Carcinoma pathology, Carcinoma surgery, Female, Goiter, Nodular surgery, Humans, Incidence, Male, Middle Aged, Nepal, Prospective Studies, Thyroid Neoplasms pathology, Thyroid Neoplasms surgery, Carcinoma epidemiology, Goiter, Nodular pathology, Thyroid Neoplasms epidemiology
- Abstract
Thyroid carcinoma (TC) is a relatively rare tumour, but it represents the most frequent form of cancer of the endocrine glands. Epidemiologically ascertained risk factors are ionising radiation, the presence of thyroid adenoma and multinodular goiter (MNG). Multinodularity of goiter should no longer be considered an indicator of probable benign disease. A prospective analysis was performed on patients operated for multinodular Goiter at Nepal Medical College from January 2009 to November 2011, in order to establish the incidence of carcinoma. The results of this study, demonstrate that in 13.63 % of the patients operated for goiter, the presence of a carcinoma was noticed in the definitive histopathologic examination. Such incidence percentage of MNG is in accordance with the data reported in published reports. Thus, we conclude that the risk of malignancy in MNG has not to be underestimated, and that a dominant nodule in MNG should be valued as if it were a solitary nodule in an otherwise normal gland.
- Published
- 2011
22. Effect of light emitting diodes in the photodynamic therapy of rheumatoid arthritis.
- Author
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Neupane J, Ghimire S, Shakya S, Chaudhary L, and Shrivastava VP
- Subjects
- Arthritis, Rheumatoid pathology, Cells, Cultured, Equipment Design, Equipment Failure Analysis, Humans, Lymphocytes pathology, Photosensitizing Agents administration & dosage, Semiconductors, Arthritis, Rheumatoid drug therapy, Lighting instrumentation, Lymphocytes drug effects, Lymphocytes radiation effects, Methotrexate administration & dosage, Photochemotherapy instrumentation
- Abstract
Background: Complex and painful surgical removal of synovium was replaced by arthroscopic synovectomy as an early treatment of rheumatoid arthritis (RA), which being limited to bigger joints, was replaced by laser synovectomy. Having been more time consuming, laser photodynamic therapy (PDT) replaced this method. Due to thermal side effects of laser PDT, an alternative source of light has been sought. Therefore, to make RA treatment cheaper, less hazardous and suitable according to anatomical geometry, light emitting diodes (LEDs) were used in this study as a potential source of light., Methods: Red, white, yellow and infra-red (IR) LEDs were tested to measure the optical penetration for soft tissue and their scattering. In vitro study of the cellular response of normal and inflamed lymphocytes from healthy and RA patients was conducted respectively. Methotrexate was injected as photosensitizer to achieve cell-specific precision., Results: IR LEDs showed the maximum penetration and least scattering of all LEDs used. Specimen with drug administration and with subsequent exposure to IR LEDs exhibited massive suppression of inflamed activated lymphocytes in comparison to other controls., Conclusion: The properly selected wavelength and intensity of light beam were incident with great precision so that they would not affect unwanted cells, but inflamed activated cells were suppressed due to intense light energy following Methotrexate injection. Without invasion, IR LED PDT showed an effective and cheaper treatment solution for RA., (2009 Elsevier B.V. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
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