Search

The datasets attached include: Local field potential (LFP) signals and action potential activity (multi-unit spikes) were recorded during the social preference, free interaction, and social fear conditioning from the Anterior Hypothalamic Nucleus (AHN) in behaving adult male mice. The LFP data is sampled at 5kHz and the multi-unit activity data includes the times of the spikes from the beginning of the recording. In addition, for each session, a video analysis file is included. Those are the final analysis results done with our previously published system (TrackRodent, https://github.com/shainetser/TrackRodent). It includes the trajectory of the mice as well as the video frames in which they investigated each of the stimuli. Finally, for each session, a Time-Stamp file is included. It presents the times of the recorded video frames relative to the LFP and spikes data, as well as the stimuli insertion and removal frames. For the optogenetics experiments, the video analysis files are included. Those are the final analysis results done with our previously published system (TrackRodent) for the different groups recorded. &nbsp

Additional file 2: Table S1. Summary of DeepSqueak performance.

Additional file 1: Figure S1. The Graphical User Interface (GUI) of the Matlab-based TrackUSF software. Figure S2. Examples and PSD profiles of various rats USF clusters. Figure S3. Vicinity curves for clusters 4-16, across the three genotypes of Shank3-deficient rats. Figure S4. Repeatability curves of clusters 4-16, for WT, Het and KO dyads.

Repeatability of behavioral results. A PDF file depicting the behavioral dynamics of two experimental groups of male (left) and female (right) mice. Each group (n = number of animals in each group) comprises animals that were tested together in the same time (one to two consecutive days). (PDF 343Â kb)

Behavioral dynamics of male and female miceâ time in compartments. A PDF file showing the behavioral dynamics of male (A, C) and female (B, D) mice in the SP (A, B) and SNP (C, D) tests, as measured from the time they spend in each virtual compartment (half of the arena). (PDF 339Â kb)

Social behaviors are crucial for human connection and belonging, often impacted in conditions like Autism Spectrum Disorder (ASD). The mesoaccumbens pathway (VTA and NAc) plays a pivotal role in social behavior and is implicated in ASD. However, the impact of ASD-related mutations on social reward processing remains insufficiently explored. This study focuses on the Shank3 mutation, associated with a rare genetic condition and linked to ASD, examining its influence on the mesoaccumbens pathway during behavior, using the Shank3 -deficient rat model. Our findings indicate that Shank3 -deficient rats exhibit atypical social interactions and have difficulty adjusting behavior based on reward values, associated with modified neuronal activity of VTA dopaminergic and GABAergic neurons and reduced dopamine release in the NAc. Moreover, we demonstrate that manipulating VTA neuronal activity can normalize this behavior, providing insights into the effects of Shank3 mutations on social reward and behavior, and identify a potential neural pathway for intervention., Competing Interests: Competing interests All authors declare no competing interests.