1. In Vivo and in Silico Trypanocidal Activity Evaluation of (-)-Cubebin Encapsulated in PLGA Microspheres as Potential Treatment in Acute Phase.
- Author
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Neres NBR, Montagnini D, Ferreira DS, Parreira RLT, Orenha RP, Lima TC, Molina EF, Cunha WR, Silva MLA, and Esperandim VR
- Subjects
- Animals, Capsules, Injections, Intraperitoneal, Lignans administration & dosage, Lignans chemistry, Male, Mice, Mice, Inbred BALB C, Molecular Docking Simulation, Parasitic Sensitivity Tests, Trypanocidal Agents administration & dosage, Trypanocidal Agents chemistry, Lignans pharmacology, Microspheres, Trypanocidal Agents pharmacology, Trypanosoma cruzi drug effects
- Abstract
In this study, the trypomastigotes of a Y strain of Trypanosoma cruzi were inoculated intraperitoneally into male BALB/c mice weighing approximately 25 g each, which were divided into groups for evaluation of the trypanocidal activity. For the treatment of experimental groups, encapsulated and unencapsulated (-)-cubebin, Benznidazole, and two groups as negative controls were used. The encapsulated (-)-cubebin showed a 68.1 % encapsulation efficiency. The parasitemia peak of substances remained around the 9
th day after the observed reduction in the number of circulating trypomastigotes. The encapsulated (-)-cubebin and (-)-cubebin unloaded showed a decrease of 61.3 % and 58.5 % in the number of parasites as compared to the negative control, respectively. Moreover, animals treated with encapsulated (-)-cubebin had a higher survival time as compared to the other groups. In conclusion, the results obtained were more promising for encapsulated (-)-cubebin as compared to unloaded particles., (© 2021 Wiley-VHCA AG, Zurich, Switzerland.)- Published
- 2021
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