5 results on '"Neng-Dang Jiang"'
Search Results
2. Biodegradation of the pyridinecarboxamide insecticide flonicamid by Microvirga flocculans and characterization of two novel amidases involved
- Author
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Yun-Xiu Zhao, Ling Guo, Li Wang, Neng-Dang Jiang, Ke-Xin Chen, and Yi-Jun Dai
- Subjects
Amidase ,Biodegradation ,Flonicamid ,Microvirga flocculans CGMCC 1.16731 ,Environmental pollution ,TD172-193.5 ,Environmental sciences ,GE1-350 - Abstract
Flonicamid (N-cyanomethyl-4-trifluoromethylnicotinamide, FLO) is a new type of pyridinecarboxamide insecticide that exhibits particularly good efficacy in pest control. However, the extensive use of FLO in agricultural production poses environmental risks. Hence, its environmental behavior and degradation mechanism have received increasing attention. Microvirga flocculans CGMCC 1.16731 rapidly degrades FLO to produce the intermediate N-(4-trifluoromethylnicotinoyl) glycinamide (TFNG-AM) and the end acid metabolite 4-(trifluoromethyl) nicotinol glycine (TFNG). This bioconversion is mediated by the nitrile hydratase/amidase system; however, the amidase that is responsible for the conversion of TFNG-AM to TFNG has not yet been reported. Here, gene cloning, overexpression in Escherichia coli and characterization of pure enzymes showed that two amidases—AmiA and AmiB—hydrolyzed TFNG-AM to TFNG. AmiA and AmiB showed only 20–30% identity to experimentally characterized amidase signature family members, and represent novel amidases. Compared with AmiA, AmiB was more sensitive to silver and copper ions but more resistant to organic solvents. Both enzymes demonstrated good pH tolerance and exhibited broad amide substrate specificity. Homology modeling suggested that residues Asp191 and Ser195 may strongly affect the catalytic activity of AmiA and AmiB, respectively. The present study furthers our understanding of the enzymatic mechanisms of biodegradation of nitrile-containing insecticides and may aid in the development of a bioremediation agent for FLO.
- Published
- 2021
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3. Biodegradation of flonicamid by Ensifer adhaerens CGMCC 6315 and enzymatic characterization of the nitrile hydratases involved
- Author
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Shi-Lei Sun, Li Wang, Yun-Xiu Zhao, Neng-Dang Jiang, Ke-Xin Chen, Feng Ge, and Yi-Jun Dai
- Subjects
Niacinamide ,Insecticides ,Calcium alginate ,Nitrile ,Bioengineering ,010501 environmental sciences ,Microbiology ,01 natural sciences ,Applied Microbiology and Biotechnology ,chemistry.chemical_compound ,Bioremediation ,Bacterial Proteins ,Rhizobiaceae ,Nitriles ,Enzymatic degradation ,Ensifer adhaerens CGMCC 6315 ,Food science ,Solubility ,Hydro-Lyases ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,Flonicamid ,biology ,Chemistry ,Research ,010401 analytical chemistry ,Biodegradation ,QR1-502 ,Enzyme assay ,0104 chemical sciences ,Transformation (genetics) ,Biodegradation, Environmental ,Enzyme ,Cell immobilization ,biology.protein ,Biotechnology - Abstract
Background Flonicamid (N-cyanomethyl-4-trifluoromethylnicotinamide, FLO) is a new type of pyridinamide insecticide that regulates insect growth. Because of its wide application in agricultural production and high solubility in water, it poses potential risks to aquatic environments and food chain. Results In the present study, Ensifer adhaerens CGMCC 6315 was shown to efficiently transform FLO into N-(4-trifluoromethylnicotinoyl) glycinamide (TFNG-AM) via a hydration pathway mediated by two nitrile hydratases, PnhA and CnhA. In pure culture, resting cells of E. adhaerens CGMCC 6315 degraded 92% of 0.87 mmol/L FLO within 24 h at 30 °C (half-life 7.4 h). Both free and immobilized (by gel beads, using calcium alginate as a carrier) E. adhaerens CGMCC 6315 cells effectively degraded FLO in surface water. PnhA has, to our knowledge, the highest reported degradation activity toward FLO, Vmax = 88.7 U/mg (Km = 2.96 mmol/L). Addition of copper ions could increase the enzyme activity of CnhA toward FLO by 4.2-fold. Structural homology modeling indicated that residue β-Glu56 may be important for the observed significant difference in enzyme activity between PnhA and CnhA. Conclusions Application of E. adhaerens may be a good strategy for bioremediation of FLO in surface water. This work furthers our understanding of the enzymatic mechanisms of biodegradation of nitrile-containing insecticides and provides effective transformation strategies for microbial remediation of FLO contamination. Supplementary Information The online version contains supplementary material available at 10.1186/s12934-021-01620-4., Highlights E. adhaerens efficiently degrades the insecticide FLO via a hydration pathway.Both free and immobilized cells effectively degrade FLO in surface water.E. adhaerens nitrile hydratases CnhA and PnhA both hydrolyze FLO to TFNG-AM.PnhA has, to our knowledge, the highest reported degradation activity toward FLO.The key residue (β-Glu56) may cause a significant difference in two NHase activities. Supplementary Information The online version contains supplementary material available at 10.1186/s12934-021-01620-4.
- Published
- 2021
4. Biodegradation of the pyridinecarboxamide insecticide flonicamid by Microvirga flocculans and characterization of two novel amidases involved
- Author
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Li Wang, Yi-Jun Dai, Yun-Xiu Zhao, Neng-Dang Jiang, Ling Guo, and Ke-Xin Chen
- Subjects
Niacinamide ,Insecticides ,Bioconversion ,Health, Toxicology and Mutagenesis ,Metabolite ,0211 other engineering and technologies ,02 engineering and technology ,010501 environmental sciences ,medicine.disease_cause ,01 natural sciences ,Environmental pollution ,Gene Expression Regulation, Enzymologic ,Amidase ,Amidohydrolases ,chemistry.chemical_compound ,Bacterial Proteins ,Nitrile hydratase ,medicine ,GE1-350 ,Microvirga flocculans ,Escherichia coli ,Microvirga flocculans CGMCC 1.16731 ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,021110 strategic, defence & security studies ,Flonicamid ,Public Health, Environmental and Occupational Health ,General Medicine ,Gene Expression Regulation, Bacterial ,Biodegradation ,Pollution ,Environmental sciences ,Enzyme ,Biodegradation, Environmental ,TD172-193.5 ,chemistry ,Biochemistry ,Methylobacteriaceae - Abstract
Flonicamid (N-cyanomethyl-4-trifluoromethylnicotinamide, FLO) is a new type of pyridinecarboxamide insecticide that exhibits particularly good efficacy in pest control. However, the extensive use of FLO in agricultural production poses environmental risks. Hence, its environmental behavior and degradation mechanism have received increasing attention. Microvirga flocculans CGMCC 1.16731 rapidly degrades FLO to produce the intermediate N-(4-trifluoromethylnicotinoyl) glycinamide (TFNG-AM) and the end acid metabolite 4-(trifluoromethyl) nicotinol glycine (TFNG). This bioconversion is mediated by the nitrile hydratase/amidase system; however, the amidase that is responsible for the conversion of TFNG-AM to TFNG has not yet been reported. Here, gene cloning, overexpression in Escherichia coli and characterization of pure enzymes showed that two amidases—AmiA and AmiB—hydrolyzed TFNG-AM to TFNG. AmiA and AmiB showed only 20–30% identity to experimentally characterized amidase signature family members, and represent novel amidases. Compared with AmiA, AmiB was more sensitive to silver and copper ions but more resistant to organic solvents. Both enzymes demonstrated good pH tolerance and exhibited broad amide substrate specificity. Homology modeling suggested that residues Asp191 and Ser195 may strongly affect the catalytic activity of AmiA and AmiB, respectively. The present study furthers our understanding of the enzymatic mechanisms of biodegradation of nitrile-containing insecticides and may aid in the development of a bioremediation agent for FLO.
- Published
- 2020
5. Nitroreduction of imidacloprid by the actinomycete Gordonia alkanivorans and the stability and acute toxicity of the nitroso metabolite
- Author
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Yi-Jun Dai, Ke-Xin Chen, Xi Cheng, Li Wang, Huo-Yong Jiang, Zhi-Ling Dai, Yun-Xiu Zhao, and Neng-Dang Jiang
- Subjects
Insecticides ,Environmental Engineering ,biology ,Chemistry ,Health, Toxicology and Mutagenesis ,Metabolite ,Daphnia magna ,Public Health, Environmental and Occupational Health ,General Medicine ,General Chemistry ,Nitroso ,Nitro Compounds ,biology.organism_classification ,Pollution ,Acute toxicity ,Actinobacteria ,Neonicotinoids ,chemistry.chemical_compound ,Biochemistry ,Biotransformation ,Toxicity ,Animals ,Environmental Chemistry ,Ecotoxicity ,EC50 - Abstract
The insecticide imidacloprid (IMI), which is used worldwide, pollutes environments and has significant ecotoxicological effects. Microbial metabolism and photolysis are the major pathways of IMI degradation in natural environments. Several studies have reported that the metabolites of IMI nitroreduction are more toxic to some insects and mammals than IMI itself. Thus, environmental degradation of IMI may enhance the ecotoxicity of IMI and have adverse effects on non-target organisms. Here, we report that an actinomycete—Gordonia alkanivorans CGMCC 21704—transforms IMI to a nitroreduction metabolite, nitroso IMI. Resting cells of G. alkanivorans at OD600 nm = 10 transformed 95.7% of 200 mg L−1 IMI to nitroso IMI in 4 d. Nitroso IMI was stable at pH 4–9. However, it rapidly degraded under sunlight via multiple oxidation, dehalogenation, and oxidative cleavage reactions to form 10 derivatives; the half-life of nitroso IMI in photolysis was 0.41 h, compared with 6.19 h for IMI. Acute toxicity studies showed that the half maximal effective concentration (EC50) values of IMI, nitroso IMI, and its photolytic metabolites toward the planktonic crustacean Daphnia magna for immobilization (exposed to the test compounds for 48 h) were 17.70, 9.38, 8.44 mg L−1, respectively. The half-life of nitroso IMI in various soils was also examined. The present study reveals that microbial nitroreduction accelerates IMI degradation and the nitroso IMI is easily decomposed by sunlight and in soil. However, nitroso IMI and its photolytic products have higher toxicity toward D. magna than the parent compound IMI, and therefore increase the ecotoxicity of IMI.
- Published
- 2022
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