1. Natural co-infection of torque teno virus and porcine circovirus 2 in the reproductive apparatus of swine
- Author
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Giseli A. Ritterbusch, Arlei Coldebella, Nelson Morés, Eraldo L. Zanella, Camila A. Sa Rocha, Janice R Ciacci-Zanella, and Neide Lisiane Simon
- Subjects
Circovirus ,Male ,Torque teno virus ,Swine ,animal diseases ,Semen ,Testicle ,Polymerase Chain Reaction ,Andrology ,medicine ,Animals ,Swine Diseases ,General Veterinary ,biology ,virus diseases ,medicine.disease ,biology.organism_classification ,Sperm ,Virology ,DNA Virus Infections ,Porcine circovirus ,medicine.anatomical_structure ,Female ,Endometritis ,Circoviridae ,Lymph ,Genital Diseases, Male ,Genital Diseases, Female - Abstract
This work aimed to detect and study natural co-infection of Circoviridae torque teno virus (TTV) and porcine circovirus 2 (PCV2) in the swine reproductive apparatus. Semen and organs from 17 boars were tested by nested and real-time PCR. PCV2 was amplified from semen (47%), lymph nodes (84.6%) and testicles (35.3%). TTV2 was amplified from 16/17 testis and 13/13 lymph nodes. TTV1 DNA was detected in fewer testicle samples (2/17), which were also TTV2 positive. Analyzed ovaries, follicular fluid and uteri of 83 culled sows showed TTV2, TTV1 and PCV2 from 49.3%, 30.1% and 6.0% of the sows, respectively. Sperm analysis indicated insignificant differences between PCV2 and TTVs positive and negative boars. The most frequent pathologic lesion in sows was endometritis (28.9%), but this was unassociated with PCV2 or TTVs detection. These findings question the importance of PCV2 and TTV2 natural co-infection in the pathology of porcine reproductive failures.
- Published
- 2012