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Your search keyword '"Negro-Demontel, María Luciana"' showing total 12 results
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12 results on '"Negro-Demontel, María Luciana"'

1. CD300f immune receptor contributes to healthy aging by regulating inflammaging, metabolism, and cognitive decline

Author

Evans, Frances, Alí-Ruiz, Daniela, Rego, Natalia, Negro-Demontel, María Luciana, Lago, Natalia, Cawen, Fabio Andrés, Pannunzio, Bruno, Sanchez-Molina, Paula, Reyes, Laura, Paolino, Andrea, Rodríguez-Duarte, Jorge, Pérez-Torrado, Valentina, Chicote-González, Almudena, Quijano, Celia, Marmisolle, Inés, Mulet, Ana Paula, Schlapp, Geraldine, Meikle, María Noel, Bresque, Mariana, Crispo, Martina, Savio, Eduardo, Malagelada, Cristina, Escande, Carlos, and Peluffo, Hugo

Published
2023
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2. CD300f immunoreceptor is associated with major depressive disorder and decreased microglial metabolic fitness

Author

Lago, Natalia, Kaufmann, Fernanda N., Negro-Demontel, María Luciana, Alí-Ruiz, Daniela, Ghisleni, Gabriele, Rego, Natalia, Arcas-García, Andrea, Vitureira, Nathalia, Jansen, Karen, Souza, Luciano M., Silva, Ricardo A., Lara, Diogo R., Pannunzio, Bruno, Abin-Carriquiry, Juan Andrés, Amo-Aparicio, Jesús, Martin-Otal, Celia, Naya, Hugo, McGavern, Dorian B., Sayós, Joan, López-Vales, Rubèn, Kaster, Manuella P., and Peluffo, Hugo

Published
2020

5. S104. Impact of CD300f Immunoreceptors in Microglia Phenotype and Behavioural Alterations Relevant to Major Depressive Disorder

Author

Lago, Natalia, primary, Kaufmann, Fernanda, additional, Negro-Demontel, María Luciana, additional, Alí-Ruiz, Daniela, additional, Vitureira, Nathalia, additional, Ghisleni, Gabriele, additional, Rego, Natalia, additional, Jansen, Karen, additional, Souza, Luciano, additional, Silva, Ricardo, additional, Lara, Diogo, additional, Pannunzio, Bruno, additional, Abin, Juan Andrés, additional, Naya, Hugo, additional, Sayós, Joan, additional, McGavern, Dorian, additional, López-Vales, Rubèn, additional, Kaster, Manuella, additional, and Peluffo, Hugo, additional

Published
2019
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8. Effect of Specific Mutations in Cd300 Complexes Formation; Potential Implication of Cd300f in Multiple Sclerosis

Author

Martínez-Barriocanal, Águeda, Arcas-García, Andrea, Magallon-Lorenz, Miriam, Ejarque-Ortíz, Aroa, Negro-Demontel, María Luciana, Comas-Casellas, Emma, Schwartz, Simo, Malhotra, Sunny, Montalban, Xavier, Peluffo, Hugo, Martín, Margarita, Comabella, Manuel, Sayós, Joan, Universitat Autònoma de Barcelona, Martínez-Barriocanal, Águeda, Arcas-García, Andrea, Magallon-Lorenz, Miriam, Ejarque-Ortíz, Aroa, Negro-Demontel, María Luciana, Comas-Casellas, Emma, Schwartz, Simo, Malhotra, Sunny, Montalban, Xavier, Peluffo, Hugo, Martín, Margarita, Comabella, Manuel, Sayós, Joan, and Universitat Autònoma de Barcelona

Abstract

Herein, we have used bioinformatics tools to predict five clusters defining ligand-binding sites on the extracellular domain of human CD300b receptor, presumably involved in the formation of both homodimers and heterodimers with other CD300 family members. Site-directed mutagenesis revealed residues glutamic acid 28 and glutamine 29 in cluster 5 to be necessary for the formation of CD300b complexes. Surprisingly, the disruption of cluster 2 and 4 reconstituted the binding capability lost by the mutation of residues glutamic acid 28 to alanine, glutamine 29 to alanine (E28A-Q29G). We identified a missense mutation arginine 33 to glutamine (R33Q) in CD300f by direct sequencing of exon 2 in peripheral blood samples from 50 patients with multiple sclerosis (MS). Levels of expression of CD300f were almost undetectable on monocytes from the patient bearing the R33Q mutation compared with healthy individuals. Whereas R33Q mutation had no effect in the formation of CD300f complexes, the inhibition of protein synthesis with cycloheximide indicated that CD300f R33Q is less stable than native CD300f. Finally, we report that the levels of expression of CD300f on the surface of classical and intermediate monocytes from MS patients are significantly lower when compared to the same cell populations in healthy individuals

Published
2017

9. Effect of Specific Mutations in Cd300 Complexes Formation; Potential Implication of Cd300f in Multiple Sclerosis

Author

Martínez-Barriocanal, Águeda, primary, Arcas-García, Andrea, additional, Magallon-Lorenz, Miriam, additional, Ejarque-Ortíz, Aroa, additional, Negro-Demontel, María Luciana, additional, Comas-Casellas, Emma, additional, Schwartz, Simo, additional, Malhotra, Sunny, additional, Montalban, Xavier, additional, Peluffo, Hugo, additional, Martín, Margarita, additional, Comabella, Manuel, additional, and Sayós, Joan, additional

Published
2017
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12. Comparative analysis of lentiviral vectors and modular protein nanovectors for traumatic brain injury gene therapy.

Author

Negro-Demontel ML, Saccardo P, Giacomini C, Yáñez-Muñoz RJ, Ferrer-Miralles N, Vazquez E, Villaverde A, and Peluffo H

Abstract

Traumatic brain injury (TBI) remains as one of the leading causes of mortality and morbidity worldwide and there are no effective treatments currently available. Gene therapy applications have emerged as important alternatives for the treatment of diverse nervous system injuries. New strategies are evolving with the notion that each particular pathological condition may require a specific vector. Moreover, the lack of detailed comparative studies between different vectors under similar conditions hampers the selection of an ideal vector for a given pathological condition. The potential use of lentiviral vectors versus several modular protein-based nanovectors was compared using a controlled cortical impact model of TBI under the same gene therapy conditions. We show that variables such as protein/DNA ratio, incubation volume, and presence of serum or chloroquine in the transfection medium impact on both nanovector formation and transfection efficiency in vitro. While lentiviral vectors showed GFP protein 1 day after TBI and increased expression at 14 days, nanovectors showed stable and lower GFP transgene expression from 1 to 14 days. No toxicity after TBI by any of the vectors was observed as determined by resulting levels of IL-1β or using neurological sticky tape test. In fact, both vector types induced functional improvement per se.

Published
2014
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