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Abstract Introduction There is a need to assess and strengthen reproductive rights-based family planning and abortion services in Indian medical schools that play a key role in medical education and service delivery. This study presents the findings of baseline assessment across nine schools in two states and one union territory with objective to assess, identify the gaps and improve the status of reproductive rights and evidence-based family planning and abortion services in Indian medical schools. Methods A convergent parallel mixed methods study was conducted in nine medical schools in Rajasthan, Gujarat, and Chandigarh a Union territory in India from October 2018 to June 2019. In-depth interviews with 33 faculty from the Department of Obstetrics and Gynaecology were conducted. The COM-B (Capability, Opportunity, and Motivation) model of behaviours was used to qualitatively identify barriers and facilitators of reproductive rights-based family planning and abortion services. Reproductive health services provided to 104 women for family planning and abortion were observed quantitatively using a pre-tested checklist. Findings Providers’ preference bias in recommending contraceptive methods to specific clients (wherein sterilisation was offered to women with two or more children and IUCD to women with one child) was observed as barrier to reproductive rights. The facilitators of rights based reproductive services included well-informed faculty regarding providing dignified and respectful care. Barriers included infrastructure gaps, high workload, insufficient human resources affecting privacy, and lower awareness and decision-making power of clients. Family planning counselling using the cafeteria approach was offered in 69.4% of cases, 31.6% of women seeking abortion services were offered counselling on both family planning and abortion. Sterilisation or IUD insertion was a pre-condition in 36.8% of women requesting an abortion. Conclusions Right-based reproductive services around family planning counselling and abortion services were delivered partially despite the medical schools’ trained faculty, mainly due to provider bias, high workload, and less autonomy and lower awareness of reproductive rights among women.

Magnetic levitation systems are complex and nonlinear, requiring sophisticated control methods to maintain the stability and position of the levitated object. This research presents an optimized fractional order PID (FOPID) control approach for position control of a freely-suspended ferromagnetic object. The dynamic system model is mathematically modeled in MATLAB using first-principle modeling and the grey box method. The FOPID controller has five degrees of freedom (DOFs) that allow for fine-tuning of the control gains and fractional orders, enabling the system to handle the nonlinearity inherent in the magnetic levitation system. The DOFs of FOPID and integer order PID controllers are optimized using the Artificial Bee Colony (ABC) algorithm and results are compared with state-of-the-art optimization methods. The results showed that the FOPID controller can effectively control the magnetic levitation system with constraints and outperforms other methods by up to 92.14% in terms of settling time with negligible steady-state error.

Background: Venous thrombosis is a common life-threatening disorder and is a significant source of morbidity and mortality in hospitalized patients. This study aims to evaluate soluble P-selectin (sP-Sel) and to correlate with D-dimer and C-reactive protein (CRP) in venous thrombosis patients. Subjects and Methods: This cross-sectional study includes 144 subjects, with cases (100) as duplex scan positive, Control A (22) includes age matched with no signs of inflammation, and Control B (22) with age matched with signs of inflammation and duplex scan negative. The severity of venous thrombosis is assessed by Well’s score. The statistical analysis was performed using Microsoft Excel, GraphPad Prism version 9.4.1 (681), and MedCalC 20.114. Normality for all the variables in controls and cases was checked. The data were expressed as the median (interquartile range). Comparison between groups was made using the Kruskal–Wallis test. Post hoc analysis was conducted using Dunn’s multiple comparison tests. Quantitative correlation of data was done using Spearman rho test. Receiver operating characteristics curves were constructed to assess the diagnostic performance of sP-Sel, and cutoff value was established. Linear and logistic regression analyses were also performed to predict the disease outcome. Results: In the study, controls have more females, but cases turned out to be more in males may be due to additional risk factors such as smoking and alcohol associated with diabetes mellitus and hypertension. The D-dimer (ng/mL) levels were compared in Control A, B, and cases, and the values were 67.5 (36–107), 135 (101–264), and 1309 (750–2390) ng/mL, respectively, and were statistically significant (P < 0.0001). The sP-Sel (ng/mL) were compared in Control A, B, and cases; the values were 7.59 (7.150–10.64), 7.84 (7.0–11.87), and 19.64 (12.95–33.29), respectively, and were statistically significant (P < 0.0001). Based on the Well’s score, the cases were categorized into having low disease probability, moderate probability, and high probability, and the sP-Sel is high in cases with high probability and is statistically significant. Based on CRP (mg/L) positive (>6 mg/L) or negative (14.29 (ng/mL) showed a sensitivity of 70% and specificity of 81.8% positively predicting the diagnosis of venous thrombosis and D-dimer at a cutoff of >367 ng/mL with a sensitivity of 91% and specificity of 95.45% is positively predicting the diagnosis of venous thrombosis. When multilinear regression analysis was performed D-dimer (ng/mL) and Well’s score were positively associated with sP-Sel (ng/mL) in cases of venous thrombosis. Logistic regression analysis is applied sP-Sel (ng/mL), D-dimer (ng/mL), Well’s score, and smoking came out as the positive predictor for the occurrence of venous thrombosis. Conclusion: D-dimer and Well’s scores are positively associated with sP-Sel and help to diagnose venous thrombosis.

Abstract The expansion of VapBC TA systems in M. tuberculosis has been linked with its fitness and survival upon exposure to stress conditions. Here, we have functionally characterized VapBC13 and VapBC26 TA modules of M. tuberculosis. We report that overexpression of VapC13 and VapC26 toxins in M. tuberculosis results in growth inhibition and transcriptional reprogramming. We have also identified various regulatory proteins as hub nodes in the top response network of VapC13 and VapC26 overexpression strains. Further, analysis of RNA protection ratios revealed potential tRNA targets for VapC13 and VapC26. Using in vitro ribonuclease assays, we demonstrate that VapC13 and VapC26 degrade serT and leuW tRNA, respectively. However, no significant changes in rRNA cleavage profiles were observed upon overexpression of VapC13 and VapC26 in M. tuberculosis. In order to delineate the role of these TA systems in M. tuberculosis physiology, various mutant strains were constructed. We show that in comparison to the parental strain, ΔvapBC13 and ΔvapBC26 strains were mildly susceptible to oxidative stress. Surprisingly, the growth patterns of parental and mutant strains were comparable in aerosol-infected guinea pigs. These observations imply that significant functional redundancy exists for some TA systems from M. tuberculosis.

Abstract Background Woolly apple aphid (Eriosoma lanigerum Hausmann) (Homoptera: Aphididae) infests the aerial and edaphic portions of apple trees. It is responsible for the serious qualitative and quantitative reduction in apple fruits. This pest is mainly managed by chemical insecticides, so this present study was focused on the isolation of native entomopathogenic fungi (EPF), studying their growth characteristics and virulence against woolly apple aphids. Results Clonostachys rogersoniana, as an EPF from an insect cadaver, was isolated and characterized, using morphological, microscopic, and molecular techniques. To the best of our knowledge, this is the first report of the occurrence of this fungus from India and it is named “C. rogersoniana isolate PUSACR01”. The radial growth and conidial yield of this isolate were excellent on PDAY among ten different culture media, whereas the relative growth rate and conidial germination were good on PDA and CA. The isolated fungus showed 85 and 86.67% mortality rates in adult females of the woolly apple aphid on day 11 at a concentration of 1.0 × 107 and 1.0 × 108 conidia ml−1, respectively. The LC50 values determined by probit regression analysis for days 7, 9, and 11 were 1.12 × 107, 2.52 × 106 , and 4.74 × 105 conidia ml−1 , respectively, while LT50 for concentrations of 1.0 × 105, 1.0 × 106, 1.0 × 107, and 1.0 × 108 conidia ml−1 were 18.73, 9.39, 6.71, and 5.03 days, respectively. Conclusion Based on the present study, C. rogersoniana would be considered a good biological control agent against the woolly apple aphid in apple orchards.

Purpose: The coronavirus disease 2019 (COVID-19) pandemic led to overall interrupted medical care, resulting in disease progression and morbidity. The study aimed to evaluate clinicopathological presentations and severity of eyelid malignancy during the COVID era, comparing the results with patients presented during pre-COVID era and evaluating the reasons for delayed presentation in patients with advanced eyelid malignancy in the COVID-era. Methods: This was a comparative, ambispective observational study. Data regarding demographic, clinical, histopathology, and treatment modalities of confirmed cases of eyelid malignancy admitted during the period from March 2020 to September 2022 (defined as COVID era group) were collected and compared with retrospectively collected data from March 2017 to March 2020 (defined as pre-COVID era group). COVID era patients were further categorized as localized and advanced disease. Patients with advanced eyelid malignancy during COVID era were further subjected to a questionnaire to evaluate the reasons for delayed presentation. Results: In total, 115 patients of eyelid malignancy were studied [COVID era group 40 (35%) and pre-COVID era group 75 (65%)]. A statistically significant increase in the duration of disease (P = 0.0001) and advanced tumor T-stage (P = 0.03) was noticed in the COVID era group. Demographic, histopathology, and ocular morbidity details were similar in both groups. The common reasons that led to delay in presentation and treatment in advanced T-stage patients during the COVID era were fear of acquiring COVID, lockdown, and lack of medical facilities. Conclusion: During the COVID-19 pandemic, both hospital- and patient-related factors contributed to delay in presentation, leading to advanced-stage disease indicating need of triaging of eyelid malignancy in the event of such pandemics in future.

Introduction: Many countries from South-East Asia reported an epidemic of sino-orbital mucormycosis (SOM), otherwise a rare disease, during the coronavirus disease 2019 pandemic. SOM, a potentially fatal disease, is typically treated with orbital exenteration and systemic antifungals after metabolic stabilization. There is no clear evidence of survival benefit of exenteration in the literature, and thus, there have been attempts at globe conserving treatments like orbital infusion after limited debridement and intraorbital injections with Amphotericin B (IOAB). Methods: We conducted a prospective comparative interventional study at a tertiary eye care hospital to evaluate treatment outcomes with the use of adjunctive IOAB in cases of SOM with mild to moderate orbital disease. Results: Thirty-six patients of SOM with mild to moderate orbital disease were recruited in the study. In the intervention group, 23/26 (885%) eyes had stable orbital disease at the end of treatment (4–6 weeks). No deterioration in visual acuity was noted as a result of treatment. In 8/26 (30.77%) patients, inflammation was noted as a side effect of IOAB requiring temporary discontinuation of injections. The mean follow-up for cases was 14.2 months (range 12–15 months). 1/23 (4.35%) patients had relapse of orbital disease at 3 months. Twenty-one patients are alive on last follow-up. Of the patients who refused treatment (controls), 2/9 (22.22%) patients relapsed. One of these patients with relapse underwent exenteration, while the other was managed with IOAB. At a follow-up of 14 months (range 12–15 months), eight patients are alive. On evaluating the ocular parameters in salvaged eyes, improvement in extraocular movements was noted in 75–80% cases. The degree of proptosis and resistance to retropulsion did not change significantly. Conclusion: In the current study, an improvement in the globe salvage rates was noted in cases of SOM with mild to moderate orbital disease treated with adjunctive IOAB as compared to controls at a mean follow-up of 14 months, although it did not achieve statistical significance. The study supports the inclusion of IOAB in routine management of mild to moderate orbital disease.

Purpose: The main factor that causes cataracts is the increased oxidative stress and imbalance of an antioxidant defense mechanism, which leads to significant changes in the lens microarchitecture. Senile cataract is the most common type of acquired cataracts due to aging. Methods: We carried out a case–control study in the biochemistry department to examine the antioxidant status (catalase and total antioxidant capacity [TAC]) and lipid peroxidation marker, that is, malondialdehyde (MDA) in human lens epithelial cells (HLECs) of different grades of senile cortical, nuclear, and posterior subcapsular cataracts. We collected 150 samples from patients aged 50–90 years. These included 50 samples of cortical cataracts, 50 of nuclear cataracts, and 50 samples of posterior subcapsular cataracts. We measured catalase activity by the Beer method, TAC by the Benzie and Strain method, and protein by the Bradford method. We also estimated TAC in the aqueous extract of HLECs by the ferric reducing ability of plasma (FRAP) method and MDA by the thiobarbituric acid assay method. Results: The results of this study showed that the level of catalase enzyme was higher in the first grade of nuclear, posterior subcapsular, and cortical cataracts than in other grades. This suggests that the catalase enzyme activity drops sharply in the second and third grades of these types of cataracts. The same pattern was observed for TAC, which was higher in the first grade of nuclear, posterior subcapsular, and cortical cataracts than in other grades. There were significant differences between catalase and TAC in different grades of cataracts, indicating that as the grading increases, both catalase and TAC decrease. Conclusion: The results of this study showed that the levels of MDA were higher and the levels of catalase and TAC were lower in patients with more severe cataracts compared to the healthy controls.

This was a prospective, interventional, comparative study conducted on patients with congenital simple severe ptosis. A novel method of silicone rod fixation to the tarsus (tarsal tunnel technique, group 1) was done compared with a conventional technique of silicone rod fixation (suture fixation technique, group 2) in frontalis suspension surgery. A total of 30 patients were randomized into two groups of 15 patients each. Postoperatively, MRD1, vertical palpebral aperture, and eyelid fold height were comparable in both groups till the last follow-up with stability in eyelid position. Patient satisfaction scores showed similar results with good-fair satisfaction grading in 13 patients in group 1 and 11 patients in group 2 at 6 months follow-up. No significant complications occurred in either group. Tarsal tunnel fixation of silicone rods is a novel method in sling surgery with optimal cosmetic and function outcomes. Further long-term studies are needed to validate the results of the technique.

Cardiac resonance is a complicated phenomenon involving the coordinated oscillations of numerous circulatory system components, such as electrical activity, contraction and relaxation, and blood flow. It is critical for the normal functioning of the heart and for maintaining blood flow throughout the body. Cardiac resonance is defined as a series of tiny waves produced by the heartbeat and overlaid on flow data and airway pressures. A variety of technologies, including cardiac magnetic resonance (CMR) imaging, can be used to identify these waves. CMR is a strong noninvasive method for seeing and quantifying heart anatomy and function in great detail. CMR can be used to assess cardiac resonance in both healthy and heart disease patients. A regular and coordinated pattern of oscillations characterizes cardiac resonance in healthy persons. In patients with heart illness, however, cardiac resonance can be interrupted, resulting in diminished cardiac function and decreased blood flow. The intricate role of cardiac resonance in cardiac health and disease is continuously being studied by researchers. However, it is obvious that cardiac resonance is an exciting area of research that has the potential to change the way to identify and treat heart illness. Yoga is a mind–body practice that has been demonstrated to have numerous cardiovascular health advantages, such as improved heart function, reduced inflammation, and lower blood pressure. Yoga is hypothesized to promote cardiac resonance by encouraging coordinated oscillations of numerous cardiovascular system components. Various researches have shown buoyant results such as yoga can be helpful in improving heart rate variability, cardiac resonance and reducing arterial stiffness. Stress can disrupt cardiac resonance and increase the risk of heart disease. More research is needed to completely understand the mechanisms that impact cardiac resonance and the long-term advantages of yoga for heart health.

Abstract Ultra-wideband (UWB) technology is extensively used in indoor navigation, medical applications, and Internet of Things devices due to its low power consumption and resilience against multipath fading and losses. This paper examines a multiple-input multiple-output (MIMO), circularly polarized (CP) dielectric resonator antenna for UWB systems. Compact form factor, high gain, wideband response, improved port isolation, and high data rates are the major design goals. This arrangement consists of two identical DRAs with self-decoupled orthogonal orientations eliminating the need for extra decoupling structures while achieving an impressive maximum isolation of 43 dB. The corner-edge feeding mechanism of the extended feedline generates two orthogonal E-fields, facilitating circular polarization. Additionally, a printed hook-shaped stub integrated with the ground plane enhances CP performance across the two operating bands without altering the DR structure. Fabrication and testing exhibit an impressive 133 $$\%$$ % impedance bandwidth (2.5–14 GHz) with high port isolation. For a 3 dB axial ratio reference, the single-element design exhibits axial ratio bandwidths (ARBW) of 1.2 GHz (3.6–4.8 GHz) and 0.8 GHz (9.3–10.1 GHz). Remarkably, the MIMO configuration achieves a single ARBW of 0.5 GHz (3.9–4.4 GHz). Detailed investigations of MIMO performance parameters, including diversity gain, envelope correlation coefficient, channel capacity loss, and total active reflection coefficient, underscore the design’s efficacy, making it a good choice for UWB wireless applications.

Perhaps the most traumatic experience for a parent is watching their child suffer in front of their eyes. Parenting, by its very nature, is a stressful activity; however, the stress of caring for an autistic child along with the myriad challenges it brings, can take a toll on parents’ health. To meet the complex demands of a child with Autism Spectrum Disorder (ASD), families can suffer from emotional, financial, and even physical burdens, which in turn leave them with little time for self-care and can lead to mounting sleep deprivation. In this review, an attempt has been made to understand the various challenges and stresses faced by the parents of a child with ASD. This review specifically focuses on mental health challenges, lack of sufficient time for self-care, problems related to communication, challenges associated with caring for an autistic child, stigmatisation related to mental health disorders, and the financial burdens faced by the parents of autistic children.

Abstract Background Mesenchymal Stromal Cells (MSCs) are the preferred candidates for therapeutics as they possess multi-directional differentiation potential, exhibit potent immunomodulatory activity, are anti-inflammatory, and can function like antimicrobials. These capabilities have therefore encouraged scientists to undertake numerous preclinical as well as a few clinical trials to access the translational potential of MSCs in disease therapeutics. In spite of these efforts, the efficacy of MSCs has not been consistent—as is reflected in the large variation in the values of outcome measures like survival rates. Survival rate is a resultant of complex cascading interactions that not only depends upon upstream experimental factors like dosage, time of infusion, type of transplant, etc.; but is also dictated, post-infusion, by intrinsic host specific attributes like inflammatory microniche including proinflammatory cytokines and alarmins released by the damaged host cells. These complex interdependencies make a researcher’s task of designing MSC transfusion experiments challenging. Methods In order to identify the rules and associated attributes that influence the final outcome (survival rates) of MSC transfusion experiments, we decided to apply machine learning techniques on manually curated data collected from available literature. As sepsis is a multi-faceted condition that involves highly dysregulated immune response, inflammatory environment and microbial invasion, sepsis can be an efficient model to verify the therapeutic effects of MSCs. We therefore decided to implement rule-based classification models on data obtained from studies involving interventions of MSCs in sepsis preclinical models. Results The rules from the generated graph models indicated that survival rates, post-MSC-infusion, are influenced by factors like source, dosage, time of infusion, pre-Interleukin-6 (IL-6)/ Tumour Necrosis Factor- alpha (TNF-α levels, etc. Conclusion This approach provides important information for optimization of MSCs based treatment strategies that may help the researchers design their experiments.

Abstract The study investigates how microfinance interventions and the entrepreneurial engagement of Self-Help Group (SHGs) members impact women’s social and economic empowerment in rural communities. The study employs a mixed-methods approach, combining quantitative data analysis and qualitative insights gathered through surveys, interviews, and case studies. The study’s findings demonstrated a notably positive and moderately significant impact of microfinance and entrepreneurial engagement on the social, economic, and psychological dimensions of women’s empowerment. Furthermore, the inclusion of case studies provided evidence that microfinance activities and entrepreneurship involvement have substantially empowered women across various domains, such as increasing financial independence, enhancing participation in decision-making processes, fostering social networks and support, and boosting their overall self-confidence and well-being. These insights emphasise the transformative potential of microfinance initiatives in advancing gender equality and women’s empowerment in diverse socio-economic contexts.

Introduction: The cornea of the eye is the most vulnerable organ to external insult. Contact lens is no exception. Changes in corneal epithelium, stroma, and loss of hexagonal shape in the endothelium have been taught in contact lens classes. We wanted to understand the cell density of endothelium in prolonged contact lens wear. Methods: It was a prospective, observational, cross-sectional study. The volunteers were divided into two groups: group 1 wore their contact lenses for over 5 years and group 2 never wore contact lenses. Specular microscopy was performed using Topcon SP-3000P. Computerized morphometry was used to evaluate the mean cell density of the corneal endothelial cells. Result: A statistically significant decrease in corneal endothelial cell density was observed in contact lens wearers. Conclusion: The use of contact lenses for a prolonged period significantly decreases corneal endothelial cell density. Suitable intervention may prevent such changes in the cornea.

This review delves into the effect of cardio, aerobic, Zumba, and yoga on physical, mental, and emotional health. Cardiovascular exercises enhance heart strength, aid weight management, boost lung capacity, increase endurance, and positively impact metabolism. Aerobic exercise contributes to heart health, lowers blood pressure, and promotes mental wellness. Zumba a dynamic dance-fitness program offers cardiovascular benefits, calorie burning, muscle toning, enhanced coordination, and stress reduction, fostering both physical and social well-being. Yoga, a holistic practice, improves flexibility, strengthens muscles, enhances balance, supports joint health, and cultivates good posture. Additionally, yoga positively influences mental and emotional health by promoting relaxation, reducing anxiety, enhancing focus, and fostering self-awareness. The combination of scientific insights across these diverse exercise modalities offers a holistic understanding of how physical activity profoundly shapes our overall well-being, making this review paper a valuable resource for individuals and health-care professionals alike.

Abstract This review explores the therapeutic potential of propolis, a natural resin produced by bees, by analyzing recent literature spanning from 2005 to 2023. Various databases including Google Scholar, Web of Science, Science Direct, PubMed, SCOPUS, and WoS were searched to uncover recent findings. Propolis exhibits a diverse array of pharmacological properties, including antimicrobial, anti-inflammatory, antioxidant, immunomodulatory, and wound-healing effects. It shows promise in treating respiratory infections, oral diseases, gastrointestinal disorders, and skin conditions. Additionally, recent research suggests its potential as an anticancer agent. The review also investigates efficacy of propolis in addressing antiviral, antibacterial, antioxidant, immunomodulatory, gastrointestinal diseases, metabolic disorders (diabetes), reproductive disorder (PCOS), cardiovascular diseases, hepatotoxicity, and wound healing properties of propolis. Hence, propolis could emerge as a natural remedy with significant potential for enhancing healthcare outcomes.

Abstract Carrot waste is a rich source of pro-vitamin A, β-carotene, recognized for its immunomodulatory and health-promoting properties. The present study was aimed at formulating an innovative table spread (ITS) enriched with concentrated carotenoid extract derived from carrot pomace. Prior extraction of carotenoids in flaxseed oil was optimized resulting in extract (CE) rich in carotenoids (82.66 µg/g) and alpha-linolenic acid (ALA). The effects of three ingredients i.e. CE, tri-sodium citrate (TC), and common salt (CS) on ITS formulated using 33 factorial experiment (27 treatment combinations) were determined employing three-way ANOVA of Proc GLM method of SAS 9.3 for its textural and sensory acceptability. The optimized formulation contained 15% CE, 1.0% CS and 0.3% TC. ITS displayed medium (≈51% fat) internal phase emulsion (confirmed via fluorescence microscopy) stabilized (-56.867 mV ± 0.850 zeta potential) by whey protein concentrate and possessed high thermal stability (studied through thermogravimetric analysis). The control table spread (CTS) was prepared using all ingredients except CE replaced with flaxseed oil. ITS showed significantly (p 0.05) difference existed for proximate composition and acidity. ITS turned out to be a naturally colored functional product and excellent source of carotenoids (24.276 μg/g ± 0.076) and ALA (13.816 g/ 100 g fat).

Fanconi anemia (FA), dyskeratosis congenita-related telomere biology disorders (DC/TBD), and Diamond–Blackfan anemia (DBA) are inherited bone marrow failure syndromes (IBMFS) with high risks of bone marrow failure, leukemia, and solid tumors. Individuals with FA have reduced fertility. Previously, we showed low levels of anti-Müllerian hormone (AMH), a circulating marker of ovarian reserve, in females with IBMFS. In males, AMH may be a direct marker of Sertoli cell function and an indirect marker of spermatogenesis. In this study, we assessed serum AMH levels in pubertal and postpubertal males with FA, DC/TBD, or DBA and compared this with their unaffected male relatives and unrelated healthy male volunteers. Males with FA had significantly lower levels of AMH (median: 5 ng/mL, range: 1.18–6.75) compared with unaffected male relatives (median: 7.31 ng/mL, range: 3.46–18.82, P = 0.03) or healthy male volunteers (median: 7.66 ng/mL, range: 3.3–14.67, P = 0.008). Males with DC/TBD had lower levels of AMH (median: 3.76 ng/mL, range: 0–8.9) compared with unaffected relatives (median: 5.31 ng/mL, range: 1.2–17.77, P = 0.01) or healthy volunteers (median: 5.995 ng/mL, range: 1.57–14.67, P < 0.001). Males with DBA had similar levels of AMH (median: 3.46 ng/mL, range: 2.32–11.85) as unaffected relatives (median: 4.66 ng/mL, range: 0.09–13.51, P = 0.56) and healthy volunteers (median: 5.81 ng/mL, range: 1.57–14.67, P = 0.10). Our findings suggest a defect in the production of AMH in postpubertal males with FA and DC/TBD, similar to that observed in females. These findings warrant confirmation in larger prospective studies.

Background: Subarachnoid spread of local anesthetics is quite erratic and is determined by several factors. There is a high predictive for the spread of spinal anaesthesia in terms of abdominal girth and vertebral column length. Our study is designed to determine the effect of abdominal girth and vertebral column length with cephalad spread of spinal anaesthesia for a given dose of 0.5% hyperbaric bupivacaine in Nepalese patients. Materials and Methods: In this prospective observational study, a total 100 patients of ASA PS I-II, aged16-65 years and undergoing lower extremity surgery under spinal anesthesia were enrolled. 2.8 mL of 0.5% hyperbaric bupivacaine was injected intrathecally at L3-4 interspace in sitting position. Extent of sensory blockade was assessed for 60 min. Simple linear regression, multiple linear regression and Pearson’s correlation coefficient analysis were performed to determine the correlation between patient variables viz abdominal girth, vertebral column length, height, weight, body mass index and age and maximum sensory block height. Results: Abdominal girth and vertebral column length correlated significantly with the maximal sensory block height after intrathecal administration of a fixed dose of 0.5% hyperbaric bupivacaine by all the three statistical tools used. Conclusion: Abdominal girth and vertebral column lengthcorrelate significantly with the maximal sensory block height in Nepalese patients receiving a fixed dose subarachnoid block with 0.5% hyperbaric bupivacaine

Background: The incidence of meningoencephalitis (ME) in India is poorly understood, and the exact etiological diagnosis is often not possible. This study was planned to elucidate the bacterial and viral etiological diagnosis of ME in children less than 5 years of age. Materials and Methods: The present study was conducted in Virus Research and Diagnostic Laboratory (VRDL), Department of Microbiology, King George’s Medical University, Lucknow, from July 2020 to June 2022. Serum, cerebrospinal fluid (CSF), and nose/throat swabs were collected from all the enrolled cases of meningoencephalitis in children below 5 years of age and tested for various etiological agents by ELISA and/or real-time PCR. Results: Of 130 enrolled cases, 50 (38.5%) cases tested positive for one or more etiological agents. Etiological agents of ME detected were Japanese encephalitis virus (JEV) (8.46%), adenovirus (6.92%), influenza virus (5.38%), dengue virus (3.85%), Parvo B-19 virus (3.08%), Orientia tsutsugamushi (3.08%), Herpes Simplex Virus-1 (HSV-1) (1.54%), measles virus (1.54%), and Varicella-Zoster Virus (VZV) (1.54%). Rubella virus, Chikungunya virus (CHKV), Mumps virus, Enteroviruses, Parecho virus, John Cunningham virus (JC), BK virus, Nipah virus, Kyasanur Forest Disease virus (KFD), Chandipura virus, Herpes Simplex Virus (HSV-2), SARS CoV-2, N. Meningitides, and H. Influenzae were tested but not detected in any of the cases. Conclusion: We identified the etiological agents in 50/130 (38.5%) suspected ME cases in children less than 5 years of age, using molecular and ELISA-based diagnostic methods. The four most common pathogens detected were JEV, adenovirus, influenza virus, and dengue virus.

Abstract Ulcerative colitis (UC) is a recurring autoimmune disorder characterized by persistent inflammation in the mucosal lining of the lower part of the large intestine. Conventional treatment options such as salicylates, corticosteroids, and immunosuppressants often come with severe side effects, limited bioavailability, and the development of drug resistance, which hampers their therapeutic effectiveness. Therefore, it is imperative to explore natural strategies as safe and alternative treatments for UC. Currently, around 40% of UC patients find relief through natural constituents, which can help reduce toxic side effects and maintain clinical remission. This review aims to provide a summary of both preclinical and clinical evidence supporting the efficacy of various natural substances in the prophylaxis of UC. These natural options include plant extracts, essential oils, nutraceuticals, and phytochemicals. Furthermore, we will delve into the potential mechanisms that underlie the protective and curative actions of these novel herbal agents. In summary, this review will explore the effectiveness of natural remedies for UC, shedding light on their preclinical and clinical findings and the mechanisms behind their therapeutic actions. These alternatives offer hope for improved treatment outcomes and reduced side effects for individuals suffering from this challenging autoimmune condition. Graphical Abstract

Abstract Background Glioblastoma (GBM) is the most aggressive among the tumors of the central nervous system (CNS), and has a dismal prognosis. Altered metabolism, especially the increased rate of aerobic glycolysis promotes rapid proliferation of GBM cells. Here, we investigated the role of aldehyde dehydrogenase 5 family member A1 (ALDH5A1), a mitochondrial enzyme in the aspect of GBM metabolism. We also studied the regulatory mechanisms of altered ALDH5A1 expression in GBM. Approach and results We show that ALDH5A1 is significantly downregulated in GBM patients in a grade dependent manner as compared to control brain and its low expression is associated with poor prognosis. It is significantly downregulated under hypoxia and is a direct target of the hypoxia induced microRNA: miR-210. Ectopic overexpression of ALDH5A1 in GBM cell lines U-87 MG and T98G markedly reduced their proliferation, 3D spheroid forming ability, and formation of reactive oxygen species (ROS). ALDH5A1 upregulation increased the oxygen consumption rate (OCR), and reduced the extracellular acidification rate (ECAR) of GBM cells while miR-210 overexpression showed the opposite. A significant downregulation in the transcript levels of LDHA, PDK1, and SLC2A1; coupled with lower glucose uptake and lactate production upon ALDH5A1 overexpression reveals that ALDH5A1 significantly reduces the glycolytic capacity of GBM cells. Total ATP generated in 24 h was more when miR-210 was overexpressed, while a slight decrease in ATP formation was observed upon ALDH5A1 upregulation. Interestingly, we also observed that ALDH5A1 expression is elevated and miR-210 levels are downregulated in IDH-mutant glioma as compared to its wild-type form. Conclusion Overall, our findings suggest that miR-210 mediated downregulation of ALDH5A1 plays a critical role in tumor metabolism and helps maintaining a high glycolytic phenotype in GBM.

Abstract Background SARS-CoV-2 infection in pregnant women during the third trimester resulted in overall adverse pregnancy outcomes compared to non-infected controls and a unique humoral and cellular response at delivery. In this study we aimed to assess the impact of SARS-CoV-2 infection on maternal/neonatal peripartum outcomes andimmunological profiles. Method In this study, we recruited 304 SARS-CoV-2 infected pregnant women and 910 SARS-CoV-2 non-infected pregnant women who were admitted for delivery. Peripartum and neonates’ outcomes response to SARS-CoV-2 infection were analyzed. Furthermore, we characterized the antibody and cytokines profile in SARS-CoV-2 infected maternal blood (MB) and cord blood (CB). We also assessed routine laboratory tests and liver function tests in MB before labor. Unpaired T test, Mann-Whitney test and Spearman test were used to analyze the data. Results SARS-CoV-2 infected pregnant women were significantly associated with increased risk of adverse pregnancy outcomes, including preterm labor (13.8% vs. 9.5%, p = 0.033) and meconium-stained amniotic fluid (8.9% vs. 5.5%, p = 0.039). The risk of low birth weight (

Abstract Appropriate characterization of reservoir properties and investigation of the effect of these properties on microbial metabolism and oil recovery under simulated reservoir conditions can aid in development of a sustainable microbial enhanced oil recovery (MEOR) process. Our present study has unveiled the promising potential of the hyperthermophilic archaeon, identified as Thermococcus petroboostus sp. nov. 101C5, to positively influence the microenvironment within simulated oil reservoirs, by producing significant amounts of metabolites, such as biosurfactants, biopolymers, biomass, acids, solvents, gases. These MEOR desired metabolites were found to cause a series of desirable changes in the physicochemical properties of crude oil and reservoir rocks, thereby enhancing oil recovery. Furthermore, our study demonstrated that the microbial activity of 101C5 led to the mobilization of crude oil, consequently resulting in enhanced production rates and increased efficiency in simulated sand pack trials. 101C5 exhibited considerable potential as a versatile microorganism for MEOR applications across diverse reservoir conditions, mediating significant light as well as heavy oil recovery from Berea/carbonaceous nature of rock bearing intergranular/vugular/fracture porosity at extreme reservoir conditions characterized by high temperature (80–101 °C) and high pressure (700–1300 psi). Core flood study, which truly mimicked the reservoir conditions demonstrated 29.5% incremental oil recovery by 101C5 action from Berea sandstone at 900 psi and 96 °C, underscoring the potential of strain 101C5 for application in the depleted high temperature oil wells.

Introduction: The uniqueness of the human dentition serves its wide acceptance in the identification of individual in forensic science. Aim and Objectives: To estimate the accuracy of age by using modified Kvaal’s formula and coronal pulp cavity index (CPCI) and compare the standard error and establish the technique among the Pune population. Materials and Methods: Four hundred cone-beam computed tomography (CBCT) scans were randomly selected with satisfying the established criteria. The second premolar was included in the study and the maximum length of tooth, root, and pulp was measured using modified Kvaal’s method and CPCI by using Planmeca CBCT machine with Romexis-three-dimensional imaging software. Results: The results were analyzed using the Chi-square test and ANOVA. Modified Kvaal’s formula was found to be the better followed by CPCI in the estimation of age among Indians with lesser error in prior. Conclusion: Modified Kvaal’s formula is precise, noninvasive, and most reliable biomarker for age estimation than tooth-coronal index.

Background: Head-and-neck cancer and its treatment can decrease the quality of life (QoL) of patients in consequence of the loss of structural and functional integrity of the oro- and nasopharyngeal region. Maxillofacial prosthetic treatment is advised when anatomical structures of the head and neck are not replaceable by living tissue, when recurrence is likely, when radiotherapy is administered, or when fragment of fractured bones are severely displaced. The aim of the study was to examine whether prosthetic rehabilitation of maxillofacial defects affects the QoL of patients using the University of Washington (UW)-QoL. Materials and Methods: This was the prospective interventional study using pre- and post-design using a convenience sampling method. The prosthesis was fabricated as per the clinical situation using heat-polymerized polymethyl methacrylate, maxillofacial silicone, or a combination of both. Results: The overall mean composite QOL score before rehabilitation was 68.05 ± 16.98 and after rehabilitation, it was 73.22 ± 11.28. Chewing and saliva (dryness of the mouth) had the lowest scores (55 ± 9.2, 62 ± 8.6, respectively) among all domains, whereas pain and anxiety had the highest scores (93 ± 3.7, 95 ± 1.5, respectively). The domains of appearance, speech, swallowing, chewing, saliva, and overall QoL showed statistically significant improvements postprosthetic rehabilitation. Conclusions: In the present study and selected population, the subjective analysis using UW-QOL showed that despite altered anatomy following resection and reconstruction, compromised physiology following chemoradiation, the oral cancer patients coped well and adapted to near-normal oral status after prosthetic rehabilitation. This was evident from their improved QOL after 1 month of prosthetic rehabilitation.

In an era characterized by the ubiquity of the internet, the proliferation of online services, and the increasing frequency of cyber threats, the detection of look-alike domains has become a critical component of cybersecurity. The current paper presents an approach for the detection of look-alike domains, leveraging the power of open-source intelligence (OSINT) tools. It included gathering and analyzing a wide range of publicly available data sources, including permutations, WHOIS records, IP information, website content, Geo IP, similarity percentage, name server, and mail server records, and building a comprehensive profile of domains under investigation. Through the application of online search engines, patterns and features that distinguish legitimate domains from their deceptive counterparts were established. The analysis demonstrated that OSINT tools provided significant information about the sample domains and successfully detected 1598 registered look-alike domains among 10 sample domains using dnstwist, while OpenSquat identified 103 squatting domains, 960 active phishing websites, and 53 domains with suspicious certificates across five sample websites. The research contributes to the enhancement of cybersecurity practices by providing a cost-effective and scalable solution for identifying look-alike domains, which can serve as precursors to various online threats, including phishing attacks, malware distribution, and fraud.

Background: Severe Cognitive Impairment (CI) is a major cause of disability and dependency among the elderly worldwide, and it has a significant impact not only on individuals but also on families, communities, and societies. Early identification and management of CI are critical in geriatric medicine. Prevalence data is often scarce, and this hinders the ground-level implementation of preventive health programs. Objectives: The objective was to find the prevalence of CI and its covariates among the urban geriatric population of Haryana. Materials and Methods: This was a community-based cross-sectional study conducted among 300 geriatric participants residing in an urban area of Rohtak district, Haryana. Hindi Mental State Examination (HMSE) tool was used to assess CI. Data were analyzed using IBM Corp, released 2020, IBM SPSS statistics for windows Ver 20, Armonk, NY. Results: The prevalence of geriatric CI was found to be 27.3% and was found to be significantly associated with age, female gender, scheduled and backward caste (BC), widowhood, and low educational status and is not engaged in any occupation, low socioeconomic status, teetotalism, economic dependency, physical dependency on others, and chronic morbidity. Conclusion: Age, female gender, scheduled and backward caste, widowhood, low educational status, nil employment status, low socioeconomic status, teetotalism, economic dependency, physical dependency on others, and chronic morbidity were found to be important correlates of CI. Further analytical studies can focus on these aspects for an early targeted preventive approach.

Context: The aim of the study was to identify and monitor the circulating strains of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the samples received at our center and update the existing national and international genomic surveillance data. Introduction: SARS-CoV-2 is no exception to the basic nature of the viruses ability to change and evolve. Since its first report in December 2019 from Wuhan, China, multiple variants of the virus have emerged and been reported. Five variants of concern have been recognized and reported by the Centers for Disease Control and Prevention, which are associated with variable degrees of transmissibility and mortality. Materials and Methods: Nasopharyngeal and oropharyngeal swabs received in viral transport medium at the Viral Research Diagnostic Laboratory were processed for reverse transcription-polymerase chain reaction for SARS-CoV-2. Whole genome sequencing (WGS) was performed for selective positive samples using Oxford Nanopore sequencing technology, using MinKNOW software for data acquisition. Statistical Analysis: The clades were assigned using Nextclade v2.4.1 software. The statistical analysis was calculated using OpenEpi version 3, an open-source calculator, and two by two. Results: Variants reported over the study period included Alpha, Kappa, Delta, and Omicron. Delta dominated in the year 2021, while Omicron was the dominant variant in 2022. In both the dominant variants, asymptomatics contributed to around 30–40% of positives. Intensive care unit admissions and mortality were higher in the Delta variant, while vaccination history and travel history were higher in the patients with Omicron variant. Conclusion: The trend tracking of these variants has been important in view of public health, enabling early interventions to control the spread of the disease and foresight in preparation for the situation.

Introduction: Individuals with diabetes have higher risk of developing depression, cognitive impairment, and dementia compared to those who do not have diabetes. The present study aims to assess the level of cognitive functions and the presence of depression in diabetes patients and healthy controls. The study also explores the level of cognition among the normal control, diabetes without depression, and diabetes with depression. Methods: In the present study, the presence of depression and the level of cognitive functions of 59 cases of diabetes mellitus type-2 were compared with an age- and gender-matched control group of 40 individuals. Clinical and demographic details were recorded on a semi-structured performa. Montreal Cognitive Assessment (MoCA) and Patient Health Questionnaire-9 (PHQ-9) were applied to both diabetes patients and healthy controls to assess the level of cognitive functions and the presence of depression, respectively. Results: On applying odds ratio (OR), it was observed in the present study that there were 93.50% more chances [OR 1.935 with 95% confidence interval (CI) being 0.481–7.789] of depression among diabetic cases as compared to the control group. Similarly, the chance of MoCA score being less than 26 was twice among the diabetic group as compared to the control group (OR 2.208 with 95% CI being 0.702–6.946). On application of the Chi-square test, the association of depression was significant with HBA1C level, level of education, and presence of complications. Conclusions: Patients with diabetes had almost double the risk of developing depression and poor cognitive functions as compared to the healthy control. High HbA1C level, level of education, and presence of complication in diabetes had a positive statistical association with depression. Thus, it is advisable to investigate patients with diabetes for the presence of depression and cognitive dysfunction by applying simple tools.

Abstract Amidst the challenges posed by climate change, exploring advanced technologies like nanotechnology is crucial for enhancing agricultural productivity and food security. Consequently, this study investigated the impact of nano SiO2 (nSiO2), nano TiO2 (nTiO2) and SiO2/TiO2 nanocomposites (NCs) on 30-day-old Zea mays L. plants and soil health at concentrations of 100 and 200 ppm. Results showed that nSiO2 and nTiO2 at 100 ppm and SiO2/TiO2 NCs at both concentrations, positively influenced plant growth, with the best stimulation observed at 200 ppm of SiO2/TiO2 NCs. Improved plant growth was associated with higher chlorophyll content, photosynthetic rate, transpiration rate, stomatal conductance, rhizospheric N-fixing and phosphate solubilizing bacterial population and plant nutrient uptake. Additionally, treated plants exhibited increased cellulose and starch levels. Malondialdehyde (MDA) content was lower or similar to that of the control, except at 200 ppm of nTiO2-treated shoots. Antioxidant enzyme activities fluctuated, indicating physiological adjustments. Overall, 100 ppm of nTiO2 as well as nSiO2 and 100 and 200 ppm of SiO2/TiO2 NCs improved soil fertility and Z. mays growth, suggesting potential benefits for sustainable agriculture. The findings lay the foundation for more comprehensive investigations into the long-term fate of nanomaterials in soil and their intricate molecular-level interactions with Z. mays.

This research investigates liquid sloshing in a 3D rigid rectangular tank. The impact of rigid baffle on sloshing frequencies has been studied. The mathematical modal has been developed using potential theory. The boundary value problem has an analytical solution in terms of velocity potential with undetermined frequency. We get a system of homogeneous algebraic equations using boundary and free surface conditions. The frequencies are calculated using the non-trivial solution condition. Frequencies of baffled tank are computed for various filling levels. The effects of filling level on frequencies are identified. ANSYS software is used to report the liquid domain and rigid baffle mode forms.

Tremendous increase in anthropogenic activities and natural disasters have created long term negative impacts to the crop productivity as well as on our ecosystem. In the debate regarding the ongoing ecosystem fluctuations, there is a need to explore an efficient, cost-effective, target-oriented and less manpower-based technologies for sustainable development. Microbial engineering provides a better solution for the growth of a healthy environment and higher agricultural productivity over the existing methods and resolved the challenges worldwide related to development of sustainable agriculture and greener ecosystems. In recent years, researchers are working on the development of different advanced microbial engineering strategies such as gene editing, CRISPR/Cas9, and RNAi to enhance the potential of microorganisms towards higher plant productivity and degradation of pollutants. The present review focused on the potential applications of genetically engineered microbial inoculants for sustainable agriculture and greener ecosystem development.

Sexually transmitted infections are a common problem worldwide. A typical presentations may sometimes occur so Chancroid should always be kept in mind as a differential diagnosis while dealing with genital ulcer disease (GUD) patients as Microbiological evidence may not always be available. We present two cases with GUD. Swabs were collected from the base and margins of the penile ulcers and sent to the bacteriology laboratory for Gram staining and aerobic routine culture. Gram-stained smears revealed numerous Gram-negative coccobacilli in a typical railroad track/school of fish appearance suggestive of Haemophilus ducreyi infection. No growth was observed on aerobic routine culture. The patients were started on single-dose azithromycin as per National AIDS Control Organisation (NACO) guidelines. Remarkable clinical improvement was observed in both patients on follow-up.

COVID-19, caused by zoonotic coronavirus SARS-CoV-2, is not a first coronavirus infection, prior to this, two severe coronavirus infections were already faced by the humans at different parts of the world. COVID-19 is found to be more severe than its previous counterparts and cause respiratory syndrome along with some other pathophysiology effects. The main human protein which used by SARS causing coronavirus (SARS-CoV and SARS-CoV-2) is angiotensin-converting enzyme 2 (ACE2), a key member and regulator of RAS. Coronavirus shows a significant affinity with the ACE2, spike protein of the virus participate in this crucial interaction and initiate the infection cycle of the SARS. This ACE2 plays a very significant role in RAS, which directly affect the pathophysiology of humans, mainly of respiratory and cardiovascular diseases. Blockage or down-regulation of ACE2 can easily block the virus entry in the cells, but due to the other important role of the ACE2, the human system cannot afford its suppression or blockage. Due to its importance, it is required to understand the physiology and pathophysiological role of the ACE2, which can help to develop therapy against the SARS. This report provides a detailed account of ACE2, and help to understand about it, which will help to plan a possible way to fight against SARS-CoV-2 and other coronaviruses.

The interaction behavior of mixed surfactant-dye systems, i.e., anionic surfactant sodium dodecyl sulfate (SDS), cationic surfactant cetyl pyridinium chloride (CPC), and dye methyl orange (MO) was investigated through the allocation of micelles using conductivity measurements. Conductivity as a function of surfactant concentration was monitored to calculate the critical micelle concentration (CMC). The conductivity increased sharply as the surfactant content increased, according to the results. Moreover, the conductivity rises with temperature, whereas it falls with increased methanol (CH3OH). CH3OH-H2O mixed solvent media containing 0 %, 10 %, 20 %, and 30 % of CH3OH at 298.15, 308.15, and 318.15 K to work out the basic micelle fixation, gives significant understanding to the arrangement of the mixed surfactants. The CMC and degree of micellar dissociation (α) of SDS with CPC increase in the CH3OH and H2O mixture. The CMC and α values are increased by increasing the temperature. Various thermodynamic parameters, i.e. Standard free energy of micellization (ΔGmo), Standard enthalpy of micellization (ΔHmo), and Standard entropy of micellization (ΔSmo) were computed in SDS-CPC without MO. ΔGmo values for SDS-CPC in the presence of MO results in micellization more probable with MO.

Abstract Recent advancements in single‐molecule methods have not only made it possible to obtain precise measurements for complex biological processes but also to produce simple mathematical models for intricate biochemical mechanisms, which would otherwise be speculative. These developments have strengthened our ability to respond through mathematical modeling to concepts of protein‒protein and protein‒DNA interactions on a nanometer level and address‐related questions. In this article, we examine an intriguing biological phenomenon in which a protein and an enzyme co‐jointly encounter carcinogenic adducts during transcription. We are focusing mainly on the dysregulation of the protein involved and the possible consequences that may arise. By providing a quantum mechanical model, we have demonstrated that the presence of carcinogenic adducts in a transcriptional bubble deregulates the protein that could cause lethal mutations. Next, we present a case study to explore carcinogenesis by suggesting an alternative experimental design. Our quantum mechanical model emphasizes the use of a quantized energies approach for specific mechanisms within the living cells. Radiation‐induced carcinogenicity can be prevented if radiation interacting with tissue is not given the energies that satisfy the quantization conditions.

Background: Natural polysaccharide tara gum (TG) has been investigated for several biological uses. The current study involved the administration of imatinib, an anticancer model medication, via TG. Methods: Imatinib-modified release tablets were developed using a direct compression method with different concentrations of TG and other excipients. Compressed tablets were evaluated for physicochemical properties. Result: All formulations had an in vitro disintegration time ranging from 10–23 min. Among the formulations, F6 exhibited excellent extended-release behaviour with 72% release over 12 h. TG tablets were rich in phytoconstituents, including saponins, tannins, phenolics, flavonoids, carbohydrates, proteins, and amino acids. Conclusion: TG has potential in the drug delivery application of anticancer medications as a rate-retarding polymer.

Bowen's disease is under-recognised, in situ - squamous cell carcinoma usually presenting as a single, well demarcated, slow growing erythematous patch or plaque with a scaly or crusted surface. It has a limited potential of invasion. It mainly occurs over the sun-exposed parts of the body (typical variant). Genitals, back, finger, trunk & thigh are the peculiar sites for pigmented, verrucous, atrophic & hyperkeratotic variants which are considered as atypical variants. A total of 3 patients (2 males and 1 female; average age: 57 years) were noted over a span of one year. Rarity, atypical variants and limited case series on Bowen disease in literature prompt us to report this interesting case series.

Objectives Due to substantial regional variability in available caregiving services and supports, culture and health status among informal caregivers in the USA, the study objective was to explore how rural-urban differences in aspects of caregiving—caregiving intensity, distance to care recipient, caregiver burden, caregiver health and caregiving support—vary by US Census region (Northeast, South, Midwest and West) after accounting for other social determinants of health.Design This study was a secondary analysis of multiwave, cross-sectional study data.Setting The data were collected on a representative sample of informal, unpaid caregivers to older adults.Participants A sample of n=3551 informal caregivers from the National Study of Caregiving identified by older adult care recipients from waves 1 (2011) and 5 (2015) of the National Health and Aging Trends Study.Primary and secondary outcome measures Primary outcome measures were caregiving intensity (provided support for/with the number of activities of daily living (ADLs) and instrumental ADL (IADLs)) caregiver assisted with, hours of caregiving per month), caregiver burden (physical, emotional and financial), support services sought (types and total number), caregivers’ self-reported health and health status (individual comorbidities and a total number of comorbidities). Analyses were stratified by US Census region and rural-urban status, as defined by the US Census Bureau, of census tract of caregiver residence.Results Urban caregivers provided higher levels of ADL support in the Northeast (beta=0.19, 95% CI 0.03, 0.35) and West (beta=0.15, 95% CI 0.05,0.26) regions. Urban caregivers provided significantly higher levels of ADL support (p=0.020), IADL support (p=0.033) and total ADLs plus IADLs (p=0.013) than rural caregivers. Caregivers living in the South had higher amounts of monthly hours spent caregiving, ADL support, IADL support and combined ADLs plus IADLs and were more likely to have obesity, report poor or fair health, have heart conditions and experience emotional difficulty from caregiving (all p

IntroductionLive microfilariae (mf) and mf-derived extracellular vesicles (EVs) have been shown to modulate human antigen presenting cell (APC) function, most notably by suppressing the induction of IL-12 (and other pro-inflammatory cytokines) following activation with LPS and interferon-y. MethodsTo explore further how EVs alter human APC function, we studied the effect of mf and EVs on human elutriated monocyte-derived dendritic cells (DC) following exposure to Mf, mf-derived excretory/secretory (E/S) products, E/S depleted of EVs through ultracentrifugation and purified EVs. After demonstrating that the measurable responses induced by live mf could be recapitulated by EVs and EV-containing E/S, we next performed RNAseq analysis of human DC following exposure to live mf, EVs, E/S, or EV-depleted E/S. ResultsIn our analyses of the data for the DC, using a false discovery rate (FDR)

SGIP1 encodes a protein Src homology 3-domain growth factor receptor-bound 2-like endophilin interacting protein 1. It is involved in the regulation of clathrin-mediated endocytosis along with having a role in energy homeostasis in neuronal systems. We generated an isogenic human induced pluripotent stem cell (iPSC) line with a biallelic frameshift variant in SGIP1. This exon has been shown to be subject to alternative splicing, leading to an isoform lacking 24 amino acids that are present in the longest SGIP isoform. The newly generated iPSC line will be helpful to dissect the differential properties of the two SGIP isoforms.

BACKGROUND: Hemodialysis is the treatment of choice for end-stage renal disease but there is increasing evidence that dialysis patients have a high burden of problems including pain which may affect their quality of life. OBJECTIVES: The objective of this study was to find out the frequency of pain, its severity, and interference in patients receiving hemodialysis. METHODOLOGY: Data was collected from 218 patients from dialysis units of the Institute of Kidney Disease, Rehman Medical Institute, and Northwest General Hospital, Hayatabad Peshawar. A brief pain inventory questionnaire (BPI) was used to find out the frequency, severity, and interference of pain. RESULTS: The frequency of pain was 70.2%. The mean time period of treatment was 22.42 months (standard deviation (SD) 28.56). The pain was most commonly present in the lower limb. The calculated mean severity was 4.3 (SD 1.70) which showed most of the patients had mild pain. The mean interference of pain in general performance was calculated to be 5.6 (SD 2.10) which presented moderate interference. There was a significant correlation between mean pain severity and mean pain interference (P=0.000). Out of those 153 patients who reported pain, 119(77.78%) were taking medications for pain relief. CONCLUSION: Pain is a frequent problem reported by these patients. Out of those who had pain, greater than two third were taking medications for pain. Pain in these patients is the main cause of interference in the performance of general activities. So, it is important to manage the pain, and strategies other than medications should also be considered. KEYWORDS: Dialysis, End Stage Renal Disease, Hemodialysis, Interference, Pain

The design methodology of a Ka-band frequency doubling two cavity gyroklystron has been discussed in this paper. In this study, the amplifier’s design and simulation are accomplished by employing nonlinear analysis and validated using particle-in-cell simulation techniques. The output cavity functions in the TE02 mode at two times the input signal frequency at the second harmonic of the cyclotron frequency, while the input cavity is powered by a 17.5 GHz source and functions in the TE01 mode at the first harmonic of the cyclotron resonance. The device’s performance has been examined both in the presence and in the absence of a beam. A number of factors were taken into account in order to optimize the interaction structure dimension to reach the device’s targeted performance. By adjusting the structural features and electron beam parameters, the device’s RF output power is assessed in an effort to increase efficiency. Simulation results estimate that the device displays a gain of 21.5 dB, an efficiency of 21.6%, a power of 227 kW, and a −3 dB bandwidth of ∼0.3%.