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This technical paper introduces a novel organ preservation system based on isochoric (constant volume) supercooling. The system is designed to enhance the stability of the metastable supercooling state, offering potential long-term preservation of large biological organs at subfreezing temperatures without the need for cryoprotectant additives. Detailed technical designs and usage protocols are provided for researchers interested in exploring this field. The paper also presents a control system based on the thermodynamics of isochoric freezing, utilizing pressure monitoring for process control. Sham experiments were performed using whole pig liver sourced from a local food supplier to evaluate the systems ability to sustain supercooling without ice nucleation for extended periods. The results demonstrated sustained supercooling without ice nucleation in pig liver tissue for 24 and 48 h. These findings suggest the potential of this technology for large-volume, cryoprotectant-free organ preservation with real-time control over the preservation process. The simplicity of the isochoric supercooling device and the design details provided in the paper are expected to serve as encouragement for other researchers in the field to pursue further research on isochoric supercooling. However, final evidence that these preserved organs can be successfully transplanted is still lacking.

The research was carried out on a population of Polwarth sheep, intensely inbred belonging to the Research and Development Institute for Sheep and Goats Breeding, Palas - Constanta. Morpho-productive indices, reproduction indices, current number compared to the one acquired in 1995, inbreeding depression, intergenerational depression, inbreeding population, body weight differentiation and quantity of wool in the year of acquisition compared to 2020 were determined. From the presented data it results that the reproduction of the sheep was not affected instead in rams the body weight and the amount of wool decreased. Surprisingly, instead of shrinking and disappearing over time, the herd has increased and continues to grow, contrary to what is known to date from the effect of intense inbreeding on a breed of sheep. [ABSTRACT FROM AUTHOR]