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15. Evaluation by RT-PCR of the Nasopharyngal Carriage Rate of SARS-CoV-2 Among Travelers Leaving the Country (Senegal)

Author

Diagne R, Niane M, Ndao M, Aminata Sow, Abdoulaye Diop, Fatou Samba, Oumar Johnson, Coura Suzanne Ndione, and Famara Sané

Abstract

Introduction: The new SARS-CoV-2 coronavirus, discovered at the end of 2019, led to a pandemic as early as March 2020, affecting all areas of life, and travelers were not spared. The objective of this work was to estimate the frequency of nasopharyngeal carriage of SARS-CoV-2 by RT-PCR in travelers leaving the country (Senegal) and to determine the factors associated with this carriage. Materials and Methods: This was a cross-sectional study conducted at the National Public Health Laboratory (LNSP) of Thiès from December 2020 to February 2021. It included 500 travelers selected by the convenience non- random sampling method. Demographic and clinical data were collected using a standardized form. The RT-PCR test was performed using nasopharyngeal samples. Results: 480 patients were declared asymptomatic. The RT-PCR positivity rate was 6% overall. The majority of patients came from the Thiès region (80%).The average age of the patients was 39 years old. The majority of patients (60%) were male. 2% of travelers had at least one medical comorbidity (diabetes, asthma and / or obesity). The most frequent clinical signs were cold 10% and cough 3.3%. Conclusion: Our results showed a low proportion of COVID-19 positive travelers. These patients were mainly elderly, male and asymptomatic.

Published
2021
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16. Trypanosoma vivax: a simplified protocol for in vivo growth, isolation and cryopreservation

Author

Ndao M., Magnus E., Büscher P., and Geerts S.

Subjects
Trypanosoma vivax, cyclophosphamide, isolation, cryopreservation, Percoll, rodents, Infectious and parasitic diseases, RC109-216
Abstract

A rodent adapted clone of Trypanosoma vivax was used to infect cyclophosphamide treated mice and rats. Fresh blood containing trypanosomes, was centrifuged in a density gradient of three Percoll solutions, 1.07, 1.06, 1.05 g/ml, respectively, carefully layered on top of each other. The yields of this simple procedure for trypanosome purification were about six times higher than those obtained with the conventional anion-exchange columns. Cryopreservation of trypanosomes using glycerol yielded 90 % viable parasites, whereas using dimethylsulfoxide, a more commonly used cryoprotectant, the viability was only 35 %.

Published
2004
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25. Plant-derived virus-like particle vaccines drive cross-presentation of influenza A hemagglutinin peptides by human monocyte-derived macrophages

Author

Makarkov, A, Golizeh, M, Ruiz-Lancheros, E, Gopal, AA, Costas-Cancelas, IN, Chierzi, S, Pillet, S, Charland, N, Landry, N, Rouiller, I, Wiseman, PW, Ndao, M, Ward, BJ, Makarkov, A, Golizeh, M, Ruiz-Lancheros, E, Gopal, AA, Costas-Cancelas, IN, Chierzi, S, Pillet, S, Charland, N, Landry, N, Rouiller, I, Wiseman, PW, Ndao, M, and Ward, BJ

Abstract

A growing body of evidence supports the importance of T cell responses to protect against severe influenza, promote viral clearance, and ensure long-term immunity. Plant-derived virus-like particle (VLP) vaccines bearing influenza hemagglutinin (HA) have been shown to elicit strong humoral and CD4+ T cell responses in both pre-clinical and clinical studies. To better understand the immunogenicity of these vaccines, we tracked the intracellular fate of a model HA (A/California/07/2009 H1N1) in human monocyte-derived macrophages (MDMs) following delivery either as VLPs (H1-VLP) or in soluble form. Compared to exposure to soluble HA, pulsing with VLPs resulted in ~3-fold greater intracellular accumulation of HA at 15 min that was driven by clathrin-mediated and clathrin-independent endocytosis as well as macropinocytosis/phagocytosis. At 45 min, soluble HA had largely disappeared suggesting its handling primarily by high-degradative endosomal pathways. Although the overall fluorescence intensity/cell had declined 25% at 45 min after H1-VLP exposure, the endosomal distribution pattern and degree of aggregation suggested that HA delivered by VLP had entered both high-degradative late and low-degradative static early and/or recycling endosomal pathways. At 45 min in the cells pulsed with VLPs, HA was strongly co-localized with Rab5, Rab7, Rab11, MHC II, and MHC I. High-resolution tandem mass spectrometry identified 115 HA-derived peptides associated with MHC I in the H1-VLP-treated MDMs. These data suggest that HA delivery to antigen-presenting cells on plant-derived VLPs facilitates antigen uptake, endosomal processing, and cross-presentation. These observations may help to explain the broad and cross-reactive immune responses generated by these vaccines.

Published
2019

27. Manifestations respiratoires basses associées à la pratique de la teinture artisanale des vêtements à Dakar (Sénégal)

Author

Thiam, K., primary, Ndiaye, E.H.M., additional, Cissé, M.F., additional, Mbaye, F.B.R., additional, Niang, S., additional, Sagne, J.M.A.N., additional, Dia, S., additional, Ndao, M., additional, Ka, W., additional, Ka, T.N., additional, Soumare, P.M., additional, Diatta, A., additional, Dia Kane, Y., additional, Touré, N.O., additional, and Fall, M., additional

Published
2019
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30. Facteurs d’exacerbations aiguës (EA) de broncho-pneumopathie chronique obstructive (BPCO) à la clinique de pneumo-phtisiologie du centre hospitalier national universitaire (CHNU) de Fann, Dakar (Sénégal)

Author

Dia Kane, Y., primary, Thiam, K., additional, Diallo, M., additional, Nidaye, E.H.M., additional, Cissé, M.F., additional, Mbaye, F.B.R., additional, Dia, S., additional, Ndao, M., additional, Ka, T.N., additional, and Touré, N.O., additional

Published
2019
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32. Évaluation de l’impact de l’école de l’asthme chez les asthmatiques suivis au service de pneumo-phtisiologue du CHNU de Fann

Author

Thiam, K., primary, Dia Kane, Y., additional, Diatta, A., additional, Ndiaye, E.H.M., additional, Cissé, M.F., additional, Mbaye, F.B.R., additional, Sagne, J.M.A.N., additional, Dia, S., additional, Ndao, M., additional, Ka, W., additional, Ka, T.N., additional, Soumaré, P.M., additional, and Touré, N.O., additional

Published
2019
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33. Emphysème sous cutané et pneumomédiastin compliquant une adéno-amygdalectomie.

Author

A., Lame C., B., Loum, D., Nakoulima A., B., Diallo T., H., Tall, A., Fall, and D., Ndao M.

Abstract

Copyright of Revue Africaine de Chirurgie et Spécialités is the property of Faculty of Medicine & Biomedical Sciences, University of Yaounde I, Cameroon and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2020

42. Infections opportunistes à localisation pulmonaire chez les patients vivant avec le VIH (PvVIH) au service des maladies infectieuses du CHNU de Fann

Author

Mbaye, F.B.R., primary, Thiam, K., additional, Dia Kane, Y., additional, Touré Badiane, N.O., additional, Diatta, A., additional, Ndiaye, E.H.M., additional, Cissé, M.F., additional, Niang, S., additional, Sagne, J.M.A.N., additional, Dia, S., additional, Ndao, M., additional, and Ka, W., additional

Published
2017
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44. Apport des prélèvements per-fibroscopiques (lavage broncho-alvéolaire (LBA)/aspiration bronchique/biopsies bronchiques) dans le diagnostic des cancers bronchiques primitifs au CHNU de Fann de Dakar

Author

Mbaye, F.-B.-R., primary, Thiam, K., additional, Touré Badiane, N.-O., additional, Dia Kane, Y., additional, Diatta, A., additional, Ndiaye, E.-H.-M., additional, Cissé, M.-F., additional, Niang, S., additional, Sagne, J.-M.-A.-N., additional, Dia, S., additional, Ndao, M., additional, and Ka, W., additional

Published
2017
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46. Performance du GenXpert selon le type de prélèvements pulmonaires

Author

Thiam, K., primary, Mbaye, F.B.R., additional, Touré Badiane, N.O., additional, Dia Kane, Y., additional, Diatta, A., additional, Ndiaye, E.H.M., additional, Cissé, M.F., additional, Niang, S., additional, Sagne, J.M.A.N., additional, Dia, S., additional, Ndao, M., additional, and Ka, W., additional

Published
2017
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47. Evaluation of the immune response and protective efficacy of Schistosoma mansoni Cathepsin B in mice using CpG dinucleotides as adjuvant

Author

Ricciardi, A, Dalton, JP, and Ndao, M

Subjects
Vaccines, Synthetic, Antibodies, Helminth, Schistosoma mansoni, Schistosomiasis mansoni, Recombinant Proteins, Cathepsin B, Mice, Inbred C57BL, Mice, Interferon-gamma, Oligodeoxyribonucleotides, Adjuvants, Immunologic, Virology, Immunoglobulin G, Antigens, Helminth, Toll-Like Receptor 9, Animals, Cytokines, Prospective Studies, Parasite Egg Count, Chemokine CCL5, Immunization Schedule
Abstract

© 2014 Elsevier Ltd. Schistosomiasis is the most important human helminth infection due to its impact on public health. Worldwide, schistosomiasis is estimated to infect at least 200 million individuals while 700 million are at risk. The clinical manifestations are chronic and significantly decrease an individual's quality of life. Infected individuals suffer from long-term organ pathologies including fibrosis which eventually leads to organ failure. The development of a vaccine against this parasitic disease would contribute to a long-lasting decrease in disease spectrum and transmission. Our group has chosen to target Schistosoma mansoni Cathepsin B as a prospective vaccine candidate. The recombinant protein was tested in the presence of synthetic oligodeoxynucleotides containing unmethylated CpG dinucleotides, which are Toll-like receptor 9 agonists known to stimulate a Th1 response. This formulation conferred a 59% decrease in worm burden as well as a reduction in egg burden. Hepatic egg burden and intestinal egg burden were decreased by 56% and 54% respectively. Immunizations with the formulation elicited robust production of Sm-Cathepsin B specific antibodies, both IgG1 and IgG2c but with the latter predominating. Furthermore, splenocytes isolated from the immunized animals, compared to control animals, had increased secretion levels of key Th1 cytokines, IFN-γ and TNF-α, as well as the chemokine CCL5 when stimulated with recombinant Sm-Cathepsin B. These results highlight the potential of Sm-Cathepsin B/CpG as a vaccine candidate against schistosomiasis.

Published
2014

50. First far-infrared high-resolution analysis of the ν2 and ν4 bands of phosgene 35Cl2CO and 35Cl37ClCO.

Author

Flaud, J.-M., Kwabia Tchana, F., Lafferty, W. J., Perrin, A., Manceron, L., and Ndao, M.

Subjects
SYNCHROTRON radiation, CARBON monoxide, PHOSGENE, NUCLEAR vibrational states, HIGH resolution spectroscopy
Abstract

A Fourier transform spectrum of phosgene (Cl2CO) has been recorded in the 17.3-μm spectral region at a temperature of 180 K and at a resolution of 0.00102 cm−1 using a Bruker IFS125HR spectrometer coupled to synchrotron radiation, leading to the observation of the ν2 and ν4 vibrational bands of the two isotopologues 35Cl2CO and 35Cl37ClCO. The corresponding upper-state ro-vibrational levels were fit using a Hamiltonian model accounting for the A-type Coriolis interaction linking the rotational levels of the 21 and 41 vibrational states. In this way, it was possible to reproduce the upper-state ro-vibrational levels to within the experimental uncertainty, i.e. ∼0.30 × 10−3 cm−1. Very accurate rotational and centrifugal distortion and interaction constants were derived from the fit, together with the following band centres: ν0(ν2,35Cl2CO) = 572.526299(30) cm−1, ν0(ν4,35Cl2CO) = 582.089026(30) cm−1, ν0(ν2,35Cl37ClCO) = 568.951791(35) cm−1 and ν0(ν4, 35Cl37ClCO) = 581.758279(35) cm−1. [ABSTRACT FROM AUTHOR]

Published
2018
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