1. Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements
- Author
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Nayara Nery de Oliveira Cunha, Marina Azevedo Junqueira, Leopoldo Cosme-Silva, Laís da Silveira Terra Santos, George Augusto Veloso de Oliveira, Rafael Tobias Moretti Neto, Denismar Alves Nogueira, Maísa Ribeiro Pereira Lima Brigagão, and Ana Beatriz da Silveira Moretti
- Subjects
Biocompatible Materials ,Dental Pulp ,Dental Pulp Capping ,Pulpotomy ,Peroxidase ,Dentistry ,RK1-715 - Abstract
Objective: To evaluate the pulp tissue of rat molars after pulpotomies with mineral trioxide aggregate (MTA), BiodentineTM (BDT) and calcium hydroxide (CH) mixed with sterile saline solution (24 hours, 72 hours, 7 days and 15 days), through correlating MPO activity with active neutrophils and MMP8 activity with tissue remodeling. Material and Methods: Thirty-eight Wistar rats were randomly distributed into groups (control, I (MTA gray), II (BDT), and III (CH)) and subdivided according to the study period of 24 hours, 72 hours, 7 days or 15 days after pulpotomy. MMP8 activity was assessed through fluorescence technique, and MPO activity was determined using the MPO assay. Results: A gradual decrease of MPO and MM8 activity occurred in the group MTA over the experimental periods (p
- Published
- 2021