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Background: Structural foot disorders can widely contribute to lower limb musculoskeletal conditions. Some researchers consider them the origin of overuse injuries in lower limbs. Although their effects on electrical activities of intrinsic and extrinsic foot muscles are well-established, their impact on other lower limb muscle groups are yet to be clarified. Objectives: This study aims to identify the activation patterns of lower limb muscle groups in various foot structures. Materials and Methods: In this case control study, 45 asymptomatic male and female subjects with different foot structures (pronated, supinated, and normal) will be selected using non-random sampling. The electrical activities of the gluteus medius, vastus lateralis and medialis, biceps femoris, semitendinosus, and lateral and medial gastrocnemius muscles will be examined during a jump-landing task. Voluntary response index, including magnitude and similarity index, will be subsequently calculated. Discussion: While several studies have evaluated the activation of lower limb muscles in different foot structures, they have solely focused on foot muscles. In contrast, the present study will assess activation patterns of the global lower limb muscles using the voluntary response index.

The foot and ankle are a complex set of multiple joints with multiple degrees of freedom that play an important role in static and dynamic activities. The intrinsic and extrinsic muscles of this complex play a role in controlling the deformity of the arch; thus, the aim of this study was to investigate the effect of the intrinsic foot muscle-strengthening exercises on knee kinematic parameters in pronated foot subjects during forward jump landing.This interventional study was performed on students aged 20-30-years old with foot pronation in the School of Rehabilitation. In this study, the kinematic changes of the knee in the frontal, sagittal and transverse plane before and after 6 weeks of strengthening exercises of the intrinsic foot muscles during the forward jump-landing task were examined using a motion analyser. All data analysis was performed offline using a special software program in the MATLAB software environment.Knee angle variables have a normal distribution before and after exercise. The ICC coefficient of all variables was calculated as between 0.44 and 0.71; therefore, the reproducibility of the variables under study in this study was considered moderate to good. The knee angle on the frontal plate was 2.54 ± 2.4 before the exercises and 2.49 ± 2.14 after the exercises, and there was no significant difference between them (Plantar intrinsic foot muscle-strengthening exercises alone have no effect on the kinematic changes of the knee in people with foot pronation, or the duration of strengthening exercises should be increased during the day to see the effect of strengthening the intrinsic muscles among pronated foot subjects.

Pronated foot is one of the most important factors that may lead to musculoskeletal injuries of the lower extremities. Among all treatments for this disorder, intrinsic foot muscles strengthening exercises (IFMSE) have an important role in management of the individuals with pronated foot. Although the effect of the IFMSE is well accepted in this population; however, their impacts on foot kinetic are yet to be clarified. The present study aims to identify the effects of the IFMSE on foot kinetic parameters in pronated foot individuals during forward jump landing.In this quasi-experimental study, 20 asymptomatic male and female subjects (mean age of 22.65 ± 2.51 years) with pronated foot structures were selected by using a simple non-random sampling method. The ground reaction force (GRF), rate of loading (ROL), and the resultant vector of time to stabilization (RVTTS) were examined during a forward jump landing task by using a force plate before and after six weeks of the IFMSE.The findings showed that the following parameters were not significantly change before and after of the IFMSE: GRF (1.97 ± 0.49 vs. 2.03 ± 0.54,In the pronated feet subjects, the IFMSE alone was not capable of changing the kinetic parameters of the foot, or the duration of using these exercises. In this study, six weeks may not be enough to make changes the foot kinetic parameters and therefore these exercises should be used for a longer period of time.

Background Nonspecific low back pain (NSLBP) is a common problem that may have an effect on the level of functional ability. Imaging techniques indicated the degeneration of multifidus muscles in patients with low back pain. But its relationship with disability in NSLBP is unclear. Objective To assess the relationship between changes in multifidus muscle morphology in MRI as paraclinical data with changes in the level of disability as clinical data in patients with CNLBP, whose MRI studies are normal. Moreover, the relationship between multifidus CSA and its thickness was determined. Design Cross-sectional study. Methods A total of 45 subjects with CNLBP participated in this study. Multifidus muscle thickness and cross-sectional area (CSA) for both sides in L4-L5 and L5-S1 levels were measured with MRI and Image J software. Level of disability was assessed with Roland-Morris disability index. Results There was no significant relationship between multifidus muscle's CSA or thickness variations among the L4-L5 and L5-S1 levels and disability index score. Furthermore, Pearson's test showed significant positive relationship between thickness and CSA of muscles (p˂0.05). Conclusion The relationship between lumbar multifidus Thickness and disability in CNLBP with normal MRI study, is not proven in this study. Multifidus muscle thickness in L4-L5 or L5-S1 level can be representative of its CSA in patients with CNLBP and normal MRI.

Melanotic melanoma has high content of melanin and laser can destroy melanin-containing cells through thermal effect. In this study, the therapeutic effect of 808 nm laser therapy was investigated on B16-F10 melanoma tumor growth and tumor-bearing mice survival time. In addition, as laser can destroy melanin as the main cause of melanoma radioresistance, the effect of laser administration to enhance radiation therapy efficacy at B16-F10 cancer cells was evaluated in vitro and in vivo. Laser therapy (1 W/cm2 × 4 min) could cause significant (P

Cell surface proteins (CSPs) are an important type of protein in different essential cell functions. This study aimed to distinguish overexpressed CSPs in colorectal cancer to investigate their biomarker, prognosis, and drug resistance potential. Raw data of three datasets including 1187 samples was downloaded then normalization and differential expression were performed. By the combination of the cancer genome atlas (TCGA) clinical data, survival analysis was carried out. Information of all CSPs was collected from cell surface protein atlas. The role of each candidate gene expression was investigated in drug resistance by CCEL and GDSC data from PharmacoGX. CRC samples including 30 tumor samples and adjacent normal were used to confirm data by RT-qPCR. Outcomes showed that 66 CSPs overexpressed in three datasets, and 146 CSPs expression associated with poor prognosis features in TCGA data that TIMP1 and QSOX2 can associate with poor patient survival independently. High-risk patients illustrated more fatality than low-risk patients based on the risk score calculated by the expression level of these genes. Receiver operating characteristic curve analysis showed that 39 CSPs as perfect biomarkers for diagnosis in CRC. Furthermore, QSOX2 and TIMP1 expression levels increased in tumor samples compared to adjacent normal samples. The Drug resistance analysis demonstrated ADAM12 and COL1A2 up-regulation among 66 overexpressed CSPs caused resistance to Venetoclax and Cyclophosphamide with a high estimate, respectively. Many CSPs are deregulated in CRC, and can be valuable candidates as biomarkers for diagnosis, prognosis, and drug resistance.

Osteoarthritis is one of the most common rheumatological diseases and is also considered as one of the causes of myofascial trigger points (MtrPs). Dry needling (DN) is one of the methods used for the treatment of the MtrPs. The aim of the current study was to investigate the effects of DN on pain in participants with knee osteoarthritis (KOA).In this before and after preliminary clinical trial study, patients with mild to moderate KOA were enrolled. In one session, after determining the location of trigger points in quadriceps and gastrocnemius muscles, the patients underwent DN. The pain was evaluated at baseline, by passing 4 days and 1 month from the intervention using the Visual Analog Scale (VAS).Sixteen patients with a mean age of 56.5 (4.53) years old have completed the study and follow-up period. According to the ANOVA analysis, VAS values indicated a significant decrease in pain score at the 4The application of one session DN can lead to improvement in pain intensity in participants with mild to moderate KOA.

Background: Cervicogenic headache (CGH) is a secondary headache with a cervical source that radiates pain to the head or face. Accordingly, one reason of CGH is myofascial trigger points. The purpose of this study was to investigate the effect of one session dry needling (DN) of myofascial trigger points of the sternocleidomastoid (SCM) muscle in patients with CGH. Materials and Methods: In this before-and-after clinical trial, 16 females aged 18–60 years with a clinical diagnosis of CGH were enrolled. All of the patients received one session DN into the myofascial trigger points of the SCM muscle. Headache index (HI), headache duration, headache frequency, and headache disability index (HDI) were assessed at 2 weeks before and 2 weeks after the intervention. This study was registered in Clinical Trials as IRCT20181109041599N1. Results: One session DN into myofascial trigger points of the SCM muscle showed a significant improvement in HI (P < 0.001). Duration and frequency of headache as well as HDI significantly reduced after intervention (P < 0.001). Conclusion: One session DN into myofascial trigger points of the SCM muscle was effective on improvement of HI, headache duration, headache frequency, and HDI in patients with CGH.

Food safety is a major problem currently facing the world and food consumption has been identified as the major pathway for human exposure to hazardous pollutants such as heavy metals. These datasets include the concentration of heavy metals like Cd, Pb, Cu, Ba, Co and Sn in selected vegetables in Guilan province and estimate daily intake of metals. The results of this dataset showed that the average concentration of heavy metals including Cd, Pb, Cu, Ba, Co and Sn in total vegetables were 0.55, 1.098, 4.095, 5.98, 0.69, and 0.2 mg/kg, respectively. The mint showed higher levels of Pb, Cu and Co contamination compared to other vegetables. The estimated daily intakes of Cd, Pb, Cu, Ba, Co and Sn for children were 0.311, 0.622, 2.320, 3.388, 0.391, 0.119 µg/day, whereas for adults were 0.182, 0.363, 1.357, 1.98, 0.228, 0.069 mg/kg, respectively. The present data highlights that both adults and children consuming vegetables ingest significant amount of these metals. Keywords: Vegetables, Heavy metals, Daily intake

A high-performance sensing layer based on dual-template molecularly imprinted polymer (DMIP) was fabricated and successfully applied for one-by-one detection of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) as lung cancer biomarkers. The plastic antibodies of AFP and CEA were created into the electropolymerized polypyrrole (PPy) on a fluorine-doped tin oxide (FTO) electrode. Raman spectroscopy, field emission scanning electron microscopy (FE-SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) tests were performed to pursue the formation and characterization of the sensing layer. Methyl orange (MO) increased the conductivity of PPy and induced the formation of MO doped PPy (PPy-MO) rectangular-shaped nanotubes. Using impedimetric detection, the rebinding of the template antigens was evaluated, the charge transfer resistance increased as the concentration of AFP and CEA increased. The linear dynamic ranges of 5-10

A selective detection strategy for salicylic acid (SA) based on the electrosorption/electrodesorption process using an electrochemically controlled solid phase extraction (EC-SPME) configuration is presented. We used conducting polymer polypyrrole (PPy) bearing the anionic dopant nitrate (PPy/nitrate) as a sorbent element for the extraction of SA in plasma and urine samples. The vertically grown nanosheets conducting film was directly synthesized by cyclic voltammetry method on a rod-shaped stainless steel electrode. The prepared electrode was employed for selective electrosorption of SA from sample solutions by applying positive potential and following its successive electrodesorption by the use of negative potential. Several parameters like synthesis conditions, anion dopant effect, potential and time of electrosorption/electrodesorption, and exchange anion type were explored to achieve the optimal conditions. The morphology of nanostructure PPy/nitrate sorbent was characterized by scanning electron microscopy (SEM). The electrosorption of SA was demonstrated by using FT-IR and fluorescence microscopic imaging. The obtained linear dynamic range (LDR), the limit of detection (LOD), and RSD% of SA determination by fluorescence spectrometry were 5–100 nanomol L−1, 1.4 nanomol L−1 and 4.9 (n = 5), respectively. The suggested technique was successfully applied for the quantification and determination of trace amounts of SA in biomedical samples without any additional treatment.

Type 2 diabetes is characterized by poor glycemic control due to decreased insulin sensitivity. Physical activity plays an important role in the management of diabetes and reduces blood glucose level. The aim of this study was to evaluate the effectiveness of passive stretching (PS) on the blood glucose level (BGL) of diabetic patients.In this randomized clinical trial, fifty patients with type 2 diabetes and mean age of 50.7 ± 4.8 years were randomly and equally allocated into control and intervention groups. Patients in the intervention and control groups underwent 20 min of passive stretching (PS) and passive movement (PM), respectively. BGL was measured before and immediately after, 20 min after and 1 h after PS/PM in the two study groups. BGL at the mentioned times was compared between and within the groups.The findings showed that when compared with before the PS (195.7 ± 30.1), BGL significantly reduced (p 0.001) immediately after (178.9 ± 29.7), 20 min after (183.2 ± 29.1), and 1 h after (187.8 ± 29.6) the PS. However, BGL after PM (immediately, 20 min and 1 h after PM) did not significantly change (p 0.05).The findings of this study indicated that PS has a significant effect on the reduction of the immediate BGL in type 2 diabetic patients. The trend reduced even though the effect remained for 1 h after PS. It is therefore suggested that the effectiveness of these types of activities should be evaluated over a longer duration of study.

Titanium(IV) tetraisopropoxide was employed as a metal oxide sol-gel precursor to prepare ceramic composite nanofibers by the electrospinning system. To facilitate this process and obtain the desired nanofibers with higher aspect ratios and surface area, poly(vinylpyrrolidone) was added to the sol of titania. Four ceramic nanofibers sheets based on titania were prepared while each sheet contained different transition metals such as Fe-Mn, Fe-Ni, Fe-Co, and Fe-Mn-Co-Ni. The scanning electron microscope images showed good homogeneity for all the prepared ceramic composites with a diameter range of 100-250 nm. The sorption efficiency was investigated by a micro-solid-phase extraction setup in online combination with high-performance liquid chromatography for the determination of naproxen and clobetasol. All the prepared composites exhibited comparable efficiencies for the desired analytes and the type of metal showed insignificant effect. For the selected composite with Fe-Mn, the linearity of the analytes was in the range of 1-1000 μg/L and the limit of detection values were found to be 2 and 0.3 μg/L for naproxen and clobetasol, respectively. The developed method was extended to the analysis of urine and blood plasma samples and acceptable relative standard deviations were obtained at two concentration levels.

Pronated of the foot is one of the important factors contributing to musculoskeletal problems affecting the lower extremities. It is known that in a pronated foot, excessive mechanical load is applied to the lower limb structures which may result in altered biomechanics and muscle activation patterns. The aim of this study was to determine changes in the muscle activation pattern of the lower extremities in individuals with pronated, compared to normal, feet, using the voluntary response index (VRI).In this cross sectional study, 15 asymptomatic pronated foot individuals (mean age 23.27 ± 3.28 years) and 15 normal subjects (mean age 23.40 ± 3.11 years) were recruited by simple non-random sampling. Electrical activities of gluteus medius (GM), vastus lateralis (VL), vastus medialis (VM), biceps femoris, semitendinosus (ST), and medial gastrocnemius (MG) muscles were recorded during a forward jump landing task. Voluntary response index (VRI) variables, included similarity index (SI) and magnitude (Mag) were also evaluated.Muscle activity of VM (p 0.001) and ST (p = 0.010) were significantly higher but VL (p = 0.039) and MG (p = 0.001) were significantly lower in pronated foot, compared to normal subjects. Similarity index was found to be different (p 0.001) between pronated foot and healthy individuals. No significant difference was found in terms of Mag between the two groups (p = 0.576).The altered pattern of lower limb muscle activation identified in the pronated foot during landing may be attributed to the different activation involving VL, VM, MG and ST muscles. Adaptations to the biomechanical effects, due to the pronated foot causing altered activation of VL, VM, MG, and ST muscles, results in an altered pattern of muscle activation. This change in activation pattern may harm the effectiveness of movement control processes; and might also predispose individuals with pronated feet, to injuries. It seems that an altered motor strategy with the aim of minimizing biomechanical changes, predisposes individuals to injuries. However, further large scale studies are needed to support the findings of the present study.

Background Myofascial pain syndrome (MPS) is a common non-articular musculoskeletal disorder. It is characterized by local and referred pain due to the presence of myofascial trigger points (MTrPs). MTrPs most commonly involve the upper trapezius muscle and can be visualized using ultrasound imaging. This study was designed to determine the inter-rater reliability of some new ultrasonographic indices of the upper trapezius muscle and the sensitivity and specificity of 2D ultrasound imaging in the diagnosis of MPS. Materials and methods This semi-experimental study enrolled 15 participants of both genders (mean age: 40.60 ± 5.74 years) with suspected symptoms of MPS. In the first step of the study, the sensitivity and specificity of ultrasonography for diagnosis of MPS was determined in a double blind manner. In the second step, some ultrasonographic measurements, such as muscle thickness, area of MTrPs in longitudinal view, echogenicity of MTrPs in longitudinal view, echogenicity of muscle with MTrPs in longitudinal and transverse views, and the pennation angle of the upper trapezius muscle were measured twice and their reliability was determined using the value of the mean of the two measurements. Results The sensitivity and specificity of ultrasonographic diagnosis were 91% and 75%, respectively. The inter-rater reliability of upper trapezius muscle thickness, pennation angle, area of MTrPs, echogenicity of active MTrPs in longitudinal view, echogenicity of muscle with MTrPs in transverse view and echogenicity of muscle with MTrPs in longitudinal view were 0.91, 0.96, 0.93, 0.83, 0.93, and 0.91, respectively. Conclusions 1. Our findings indicated that ultrasonography is a useful method for the diagnosis of MPS owing to its high sensitivity. 2. Appropriate reliability of the quantitative ultrasonographic indices of interest, especially the area of MTrPs and their echogenicity, could be useful for long-term monitoring and designing interventional studies for better management of the syndrome.

Summary Introduction Myofascial pain syndrome (MPS) is a neuromuscular dysfunction consisting of both motor and sensory abnormalities. Considering the high prevalence of MPS and its related disabilities and costs, this study was designed to determine the reliability of new ultrasonographic indexes of the upper trapezius muscle as well as the sensitivity and specificity of 2D ultrasound imaging for diagnostic purposes. Furthermore, we sought to evaluate the effectiveness of dry needling (DN) on studied ultrasonographic indexes. Materials and methods This study will be performed in two steps with two different designs. The first is a pilot study and was designed as a semi-experimental study to determine the sensitivity and specificity of ultrasonography for the diagnosis of MPS and the reliability of ultrasonographic measurements like muscle thickness, area of myofascial trigger points (MTrPs) in longitudinal view, echogenicity of MTrPs in longitudinal view, echogenicity of muscle with MTrPs in longitudinal and transverse views, and pennation angle of upper trapezius muscle. The second study is an interventional study which was designed to investigate the effectiveness of DN on ultrasonographic measurements, for which the reliability was determined in the first study. Conclusion we will quantify the effectiveness of DN on MTrPs and muscle tissue by using novel ultrasonographic indexes. The results of the current study will provide baseline information to design more interventional studies to improve the evaluation of other treatments of MPS.

Titanium(IV) tetraisopropoxide was employed as a metal oxide sol-gel precursor to prepare ceramic composite nanofibers by the electrospinning system. To facilitate this process and obtain the desired nanofibers with higher aspect ratios and surface area, poly(vinylpyrrolidone) was added to the sol of titania. Four ceramic nanofibers sheets based on titania were prepared while each sheet contained different transition metals such as Fe-Mn, Fe-Ni, Fe-Co, and Fe-Mn-Co-Ni. The scanning electron microscope images showed good homogeneity for all the prepared ceramic composites with a diameter range of 100-250 nm. The sorption efficiency was investigated by a micro-solid-phase extraction setup in online combination with high-performance liquid chromatography for the determination of naproxen and clobetasol. All the prepared composites exhibited comparable efficiencies for the desired analytes and the type of metal showed insignificant effect. For the selected composite with Fe-Mn, the linearity of the analytes was in the range of 1-1000 μg/L and the limit of detection values were found to be 2 and 0.3 μg/L for naproxen and clobetasol, respectively. The developed method was extended to the analysis of urine and blood plasma samples and acceptable relative standard deviations were obtained at two concentration levels.