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3. Cell Therapy for Parkinson’s Disease: A Translational Approach to Assess the Role of Local and Systemic Immunosuppression

Author

Aron Badin, R., Vadori, M., Vanhove, B., Nerriere‐Daguin, V., Naveilhan, P., Neveu, I., Jan, C., Lévèque, X., Venturi, E., Mermillod, P., Van Camp, N., Dollé, F., Guillermier, M., Denaro, L., Manara, R., Citton, V., Simioni, P., Zampieri, P., D’avella, D., Rubello, D., Fante, F., Boldrin, M., De Benedictis, G.M., Cavicchioli, L., Sgarabotto, D., Plebani, M., Stefani, A.L., Brachet, P., Blancho, G., Soulillou, J.P., Hantraye, P., and Cozzi, E.

Published
2016
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9. Enteric glia inhibit intestinal epithelial cell proliferation partly through a TGF-[[beta].sub.1]-dependent pathway

Author

Neunlist, M., Aubert, P., Bonnaud, S., Van Landeghem, L., Coron, E., Wedel, T., Naveilhan, P., Ruhl, A., Lardeux, B., Savidge, T., Paris, F., and Galmiche. J.P.

Subjects
Epithelial cells -- Research, Colon cancer -- Risk factors, Colon cancer -- Research, Neuroglia -- Risk factors, Neuroglia -- Research, Intestine, Small -- Research, Nervous system, Autonomic -- Research, Biological sciences
Abstract

Although recent studies have shown that enteric neurons control intestinal barrier function, the role of enteric glial cells (EGCs) in this control remains unknown. Therefore, our goal was to characterize the role of EGCs in the control of intestinal epithelial cell proliferation using an in vivo transgenic and an in vitro coculture model. Assessment of intestinal epithelial cell proliferation after ablation of EGCs in transgenic mice demonstrated a significant increase in crypt cell hyperplasia. Furthermore, mucosal glial network (assessed by immunohistochemical detection of S-10013) is altered in colon adenocarcinoma compared with control tissue. In an in vitro coculture model of subconfluent Caco-2 cells seeded onto Transwell filters with EGCs, Caco-2 cell density and [[sup.3]H]thymidine incorporation were significantly lower than in control (Caco-2 cultured alone). Flow cytometry analysis showed that EGCs had no effect on Caco-2 cell viability. EGCs induced a significant increase in Caco-2 cell surface area without any sign of cellular hypertrophy. These effects by EGCs were also seen in various transformed or nontransformed intestinal epithelial cell lines. Furthermore, TGF-[[beta].sub.1] mRNA was expressed, and TGF-[[beta].sub.1] was secreted by EGCs. Exogenously added TGF-[[beta].sub.1] reproduced partly the EGC-mediated effects on cell density and surface area. In addition, EGC effects on Caco-2 cell density were significantly reduced by a neutralizing TGF-[[beta].sub.1] anti-body. In conclusion, EGCs have profound antiproliferative effects on intestinal epithelial cells. Functional alterations in EGCs may therefore modify intestinal barrier functions and be involved in pathologies such as cancer or inflammatory bowel diseases. colon cancer; enteric nervous system

Published
2007

10. Transgenic expression of CTLA4-Ig by fetal pig neurons for xenotransplantation

Author

Martin, Caroline, Plat, Martine, Nerrière-Daguin, Véronique, Coulon, Flora, Uzbekova, Svetlana, Venturi, Eric, Condé, Françoise, Hermel, Jean-Michel, Hantraye, Philippe, Tesson, Laurent, Anegon, Ignacio, Melchior, Benoit, Peschanski, Marc, Mauff, Brigitte Le, Boeffard, Françoise, Sergent-Tanguy, Solène, Neveu, Isabelle, Naveilhan, Philippe, Soulillou, Jean-Paul, Terqui, Michel, Brachet, Philippe, and Vanhove, Bernard

Published
2005
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11. Assessing the Potential Clinical Utility of Transplantations of Neural and Mesenchymal Stem Cells for Treating Neurodegenerative Diseases

Author

Lescaudron, Laurent, primary, Boyer, C., additional, Bonnamain, Virginie, additional, Fink, K. D., additional, Lévêque, X., additional, Rossignol, J., additional, Nerrière-Daguin, V., additional, Malouet, A. C., additional, Lelan, F., additional, Dey, N. D., additional, Michel-Monigadon, D., additional, Lu, M., additional, Neveu, I., additional, von Hörsten, S., additional, Naveilhan, P., additional, and Dunbar, G. L., additional

Published
2012
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19. IL-7 pathway controls human T cell homing to the gut and culminates in inflammatory bowel disease mucosa

Author

Belarif, Lyssia, Kermarec, L., Daguin, V., C., Mary, Danger, R., A., Kucik, Macdonald, T., Blancho, G., Naveilhan, P., Vanhove, Bernard, Poirier, Nicolas, Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), OSE Immunotherapeutics [Nantes, France], Institut de transplantation urologie-néphrologie (ITUN), Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), Neuropathies du système nerveux entérique et pathologies digestives, implication des cellules gliales entériques, Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), Barts & The London School of Medicine and Dentistry [London, UK] (Blizard Institute), Queen Mary University of London (QMUL), and Le Bihan, Sylvie

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience

Published
2017

21. Low-dose pesticide mixture induces senescence in normal mesenchymal stem cells (MSC) and promotes tumorigenic phenotype in premalignant MSC

Author

Heymann, D., Hochant, M., Pecqueur, C., April, P., Oliver, L., Denis, J., Brion, R., Amiaud, J., Pineau, A., Naveilhan, P., Vallette, F., and Christophe, O.

Abstract

Humans are chronically exposed to multiple environmental pollutants such as pesticides with no significant evidence about the safety of such poly-exposures. We exposed mesenchymal stem cells (MSC) to very low doses of mixture of seven pesticides frequently detected in food samples for 21 days in vitro. We observed a permanent phenotype modification with a specific induction of an oxidative stress-related senescence. Pesticide mixture also induced a shift in MSC differentiation towards adipogenesis but did not initiate a tumorigenic transformation. In modified MSC in which a premalignant phenotype wasinduced, the exposure to pesticide mixture promoted tumorigenic phenotype both in vitro andin vivo after cell implantation, in all nude mice. Our results suggest that a commoncombination of pesticides can induce a premature ageing of adult MSC, and as such couldaccelerate age-related diseases. Exposure to pesticide mixture may also promote thetumorigenic transformation in a predisposed stromal environment.

Published
2016

22. Glioplasticity in irritable bowel syndrome

Author

Lilli, N. L., primary, Quénéhervé, L., additional, Haddara, S., additional, Brochard, C., additional, Aubert, P., additional, Rolli‐Derkinderen, M., additional, Durand, T., additional, Naveilhan, P., additional, Hardouin, J.‐B., additional, De Giorgio, R., additional, Barbara, G., additional, Bruley des Varannes, S., additional, Coron, E., additional, and Neunlist, M., additional

Published
2017
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23. Effet protecteur de l’administration de membranes du globule gras du lait et L. fermentum CECT 5716 sur les fonctions digestives et sur la réponse inflammatoire dans un modèle de rat nouveau-né

Author

Vanhaecke, T., primary, Grohard, P.-A., additional, Aubert, P., additional, Jaulin, J., additional, Chevalier, J., additional, Durand, T., additional, Boudin, H., additional, Naveilhan, P., additional, Ligneul, A., additional, Fressange-Mazda, C., additional, Le Ruyet, P., additional, and Neunlist, M., additional

Published
2017
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24. L. fermentum CECT 5716prevents stress-induced intestinal barrier dysfunction in newborn rats

Author

Vanhaecke, T., primary, Aubert, P., additional, Grohard, P.‐A., additional, Durand, T., additional, Hulin, P., additional, Paul‐Gilloteaux, P., additional, Fournier, A., additional, Docagne, F., additional, Ligneul, A., additional, Fressange‐Mazda, C., additional, Naveilhan, P., additional, Boudin, H., additional, Le Ruyet, P., additional, and Neunlist, M., additional

Published
2017
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25. Renforcement de la barrière épithéliale intestinale par la souche probiotique Lactobacillus fermentum CECT 5716 chez le raton nouveau-né

Author

Vanhaecke, T., primary, Grohard, P.-A., additional, Aubert, P., additional, Jaulin, J., additional, Chevalier, J., additional, Durand, T., additional, Boudin, H., additional, Naveilhan, P., additional, Ligneul, A., additional, Fressange-Mazda, C., additional, Le Ruyet, P., additional, and Neunlist, M., additional

Published
2017
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27. Glioplasticity in irritable bowel syndrome.

Author

Lilli, N. L., Quénéhervé, L., Haddara, S., Brochard, C., Aubert, P., Rolli‐Derkinderen, M., Durand, T., Naveilhan, P., Hardouin, J.‐B., De Giorgio, R., Barbara, G., Bruley des Varannes, S., Coron, E., and Neunlist, M.

Subjects
IRRITABLE colon, FLOW cytometry, HISTAMINE receptors, GENE expression, ABDOMINAL bloating
Abstract

Abstract: Background: Growing evidence indicates a wide array of cellular remodeling in the mucosal microenvironment during irritable bowel syndrome (IBS), which possibly contributes to pathophysiology and symptom generation. Here, we investigated whether enteric glial cells (EGC) may be altered, and which factors/mechanisms lead to these changes. Methods: Colonic mucosal biopsies of IBS patients (13 IBS‐Constipation [IBS‐C]; 10 IBS‐Diarrhea [IBS‐D]; 11 IBS‐Mixed [IBS‐M]) and 24 healthy controls (HC) were analyzed. Expression of S100β and GFAP was measured. Cultured rat EGC were incubated with supernatants from mucosal biopsies, then proliferation and Ca2+ response to ATP were analyzed using flow cytometry and Ca2+ imaging. Histamine and histamine 1‐receptor (H1R) involvement in the effects of supernatant upon EGC was analyzed. Key Results: Compared to HC, the mucosal area immunoreactive for S100β was significantly reduced in biopsies of IBS patients, independently of the IBS subtype. IBS‐C supernatants reduced EGC proliferation and IBS‐D and IBS‐M supernatants reduced Ca2+ response to ATP in EGC. EGC expressed H1R and the effects of supernatant upon Ca2+ response to ATP in EGC were blocked by pyrilamine and reproduced by histamine via H1R. IBS supernatants reduced mRNA expression of connexin‐43. The S100β‐stained area was negatively correlated with the frequency and intensity of pain and bloating. Conclusion and Inferences: Changes in EGC occur in IBS, involving mucosal soluble factors. Histamine, via activation of H1R‐dependent pathways, partly mediates altered Ca2+ response to ATP in EGC. These changes may contribute to the pathophysiology and the perception of pain and bloating in patients with IBS. [ABSTRACT FROM AUTHOR]

Published
2018
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28. Xenotransplantation of porcine CTLA4-Ig+/+ neural precursors enables recovery of the locomotor activity in parkinsonian primates

Author

Badin, A.R., Denaro, L., Vadori, M., Boldrin, M., Cavicchioli, Lionel, De Benedictis, G.M., Fante, F., Sgarabotto, D., Jan, Charlie, Daguin, V., Naveilhan, P., Vanhove, B., Blancho, G., Soulillou, J.P., Plat, Martine, Venturi, Eric, Botté, Françoise, Padoan, A., Manara, R., Zampieri, P., D'avella, D., Rubello, D., Gianello, P., Hantraye, P., Cozzi, Elena, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre National de la Recherche Scientifique (CNRS), Universita di Padova, Immunointervention dans les allo et xénotransplantations, Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM)-ITUN, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), Ospedale 'Santa Maria della Misericordia' = University Hospital 'Santa Maria della Misericordia', Université Catholique de Louvain = Catholic University of Louvain (UCL), ProdInra, Migration, Università degli Studi di Padova = University of Padua (Unipd), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], XENOGREFFE, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], HISTOLOGIE, [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS, activité leucomotrice
Abstract

331; International audience

Published
2011

29. Distinct Roles of Bcl-2 and Bcl-Xl in the Apoptosis of\ud Human Bone Marrow Mesenchymal Stem Cells during\ud Differentiation

Author

Oliver, L., Hue, E., Rossignol, J., Bougras, G., Hulin, P., Naveilhan, P., Heymann, D., Lescaudron, L., and Vallette, F.M.

Subjects
endocrine system, equipment and supplies
Abstract

Background: Adult mesenchymal stem cells (MSCs) can be maintained over extended periods of time before activation and differentiation. Little is known about the programs that sustain the survival of these cells.\ud Principal Findings: Undifferentiated adult human MSCs (hMSCs) did not undergo apoptosis in response to different cell death inducers. Conversely, the same inducers can readily induce apoptosis when hMSCs are engaged in the early stages of differentiation. The survival of undifferentiated cells is linked to the expression of Bcl-Xl and Bcl-2 in completely opposite ways. Bcl-Xl is expressed at similar levels in undifferentiated and differentiated hMSCs while Bcl-2 is expressed only in differentiated cells. In undifferentiated hMSCs, the down-regulation of Bcl-Xl is associated with an increased sensitivity to apoptosis while the ectopic expression of Bcl-2 induced apoptosis. This apoptosis is linked to the presence of cytoplasmic Nur 77 in undifferentiated hMSCs.\ud Significance: In hMSCs, the expression of Bcl-2 depends on cellular differentiation and can be either pro- or anti-apoptotic. Bcl-Xl, on the other hand, exhibits an anti-apoptotic activity under all conditions.

Published
2011

30. LONG TERM CLINICAL RECOVERY OF PARKINSON'S DISEASE FOLLOWING TRANSPLANTATION OF CTLA4-IG(+) PORCINE EMBRYONIC NEURONAL CELLS IN NON HUMAN PRIMATE

Author

Badin, Ra, Padoan, A, Vadori, M, Boldrin, M, Cavicchioli, Laura, DE BENEDICTIS, GIULIA MARIA, Fante, F, Seveso, M, Sgarabotto, D, Jan, C, Daguin, V, Naveilhan, P, Neveu, I, Soulillou, Jp, Giannello, P, Vanhove, B, Plat, M, Botte, F, Venturi, E, Denaro, Luca, Manara, R, Zampieri, P, D'Avella, Domenico, Rubello, D, Ancona, Ermanno, Hantraye, P, Cozzi, E., Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre National de la Recherche Scientifique (CNRS), Universita di Padova, Consorzio per la Ricerca sul Trapianto di Organi, Partenaires INRAE, Immunointervention dans les allo et xénotransplantations, Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM)-ITUN, Université Catholique de Louvain = Catholic University of Louvain (UCL), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), Ospedale 'Santa Maria della Misericordia' = University Hospital 'Santa Maria della Misericordia', Azienda Ospedaliera di Padova, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Padova = University of Padua (Unipd), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], XENOGREFFE, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], porc transgénique, ComputingMilieux_MISCELLANEOUS
Abstract

Communication orale O-330; International audience

Published
2011

31. Expression of CTLA4-Ig Is Indispensable To Enable Clinical Recovery in Parkinsonian Primate Recipients of Porcine Neural Xenografts

Author

Badin, R.A., Padoan, A., Vadori, M., Boldrin, M., Cavicchioli, Lionel, De Benedictis, G.M., Fante, F., Sgarabotto, D., Jan, Charlie, Daguin, V., Naveilhan, P., Soulillou, J.P., Vanhove, B., Plat, Martine, Venturi, Eric, Botté, Françoise, Denaro, L., Manara, R., Zampieri, P., D'avella, D., Rubello, D., Hantraye, P., Cozzi, Elena, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), ura2210, Centre National de la Recherche Scientifique (CNRS), Neurosciences, Universita degli Studi di Padova, Consorzio per la Ricerca sul Trapianto di Organi, Partenaires INRAE, Universita di Padova, Immunointervention dans les allo et xénotransplantations, Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM)-ITUN, Centre hospitalier universitaire de Nantes (CHU Nantes), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), Ospedale 'Santa Maria della Misericordia' = University Hospital 'Santa Maria della Misericordia', Azienda Ospedaliera di Padova, Università degli Studi di Padova = University of Padua (Unipd), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
XENOGREFFE, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

Poster Board #-Session: P89-III Abstract# 1236; International audience

Published
2011
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32. Porcine embryonic xenografts transgenic for CTLA4-Ig enable longterm recovery in parkinsonian macaques

Author

Badin, Ra, Padoan, A., Vadori, M., Boldrin, M., Giulia Maria De Benedictis, Fante, F., Sgarabotto, D., Jan, C., Daguin, V., Naveilhan, P., Neveu, I., Soullilou, Jp, Vanhove, B., Plat, M., Botte, F., Venturi, F., LUCA DENARO, Seveso, M., Manara, R., Zampieri, P., Avella, Domenico D., Rubello, D., Ancona, E., Hantraye, P., Cozzi, E., Commissariat à l'énergie atomique et aux énergies alternatives (CEA), ura2210, Centre National de la Recherche Scientifique (CNRS), Universita di Padova, Consorzio per la Ricerca sul Trapianto di Organi, Partenaires INRAE, Azienda Ospedaliera di Padova, Immunointervention dans les allo et xénotransplantations, Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM)-ITUN, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), Ospedale 'Santa Maria della Misericordia' = University Hospital 'Santa Maria della Misericordia', Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration, Università degli Studi di Padova = University of Padua (Unipd), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], XENOGREFFE, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], HISTOLOGIE, [INFO] Computer Science [cs], EMBRYON TRANSGENIQUE, MACAQUE, ComputingMilieux_MISCELLANEOUS
Abstract

LB01; International audience

Published
2010

33. Transgenic porcine embryonic xenografts as a treatment for Parkinson's disease in non-human primates

Author

Aron, B.R., Padoan, A., Vadori, M., Boldrin, M., Cavicchioli, Lionel, de Benedictis, G.M., Fante, F., Seveso, M., Sgarabotto, D., Jan, Charlie, Daguin, V., Naveilhan, P., Neveu, I., Soulillou, J.P., Vanhove, B., Plat, Martine, Botté, Françoise, Venturi, Eric, Denaro, L., Manara, R., Zampieri, P., d'Avella, D., Rubello, D., Ancona, E., Hantraye, P., Cozzi, Elena, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), ura2210, Centre National de la Recherche Scientifique (CNRS), Universita di Padova, Consortium for Research in Organ Transplantation (CORIT), Azienda Ospedaliera di Padova, Immunointervention dans les allo et xénotransplantations, Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM)-ITUN, Centre hospitalier universitaire de Nantes (CHU Nantes), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), Università degli Studi di Perugia (UNIPG), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Padova = University of Padua (Unipd), Consorzio per la Ricerca sul Trapianto d'Organ = Consortium for Research in Organ Transplantation (CORIT), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Perugia = University of Perugia (UNIPG), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], XENOGREFFE, EMBRYON PORCIN TRANSGENIQUE, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

Communication orale LB IXA O-1; International audience

Published
2009

34. L. fermentum CECT 5716 prevents stress-induced intestinal barrier dysfunction in newborn rats.

Author

Vanhaecke, T., Aubert, P., Grohard, P.‐A., Durand, T., Hulin, P., Paul‐Gilloteaux, P., Fournier, A., Docagne, F., Ligneul, A., Fressange‐Mazda, C., Naveilhan, P., Boudin, H., Le Ruyet, P., and Neunlist, M.

Subjects
PSYCHOLOGICAL stress, DISEASES, INTESTINAL diseases, LABORATORY rats, FOOD allergy, COLITIS, THERAPEUTIC use of probiotics
Abstract

Background Intestinal epithelial barrier ( IEB) dysfunction plays a critical role in various intestinal disorders affecting infants and children, including the development of food allergies and colitis. Recent studies highlighted the role of probiotics in regulating IEB functions and behavior in adults, but their effects in the newborn remain largely unknown. We therefore characterized in rat pups, the impact of Lactobacillus fermentum CECT 5716 (L. fermentum) on stress-induced IEB dysfunction, systemic immune response and exploratory behavior. Methods Newborn rats received daily by gavage either L. fermentum or water. Intestinal permeability to fluorescein sulfonic acid ( FSA) and horseradish peroxidase ( HRP) was measured following maternal separation ( MS) and water avoidance stress ( WAS). Immunohistochemical, transcriptomic, and Western blot analysis of zonula occludens-1 ( ZO-1) distribution and expression were performed. Anxiety-like and exploratory behavior was assessed using the elevated plus maze test. Cytokine secretion of activated splenocytes was also evaluated. Key Results L. fermentum prevented MS and WAS-induced IEB dysfunction in vivo. L. fermentum reduced permeability to both FSA and HRP in the small intestine but not in the colon. L. fermentum increased expression of ZO-1 and prevented WAS-induced ZO-1 disorganization in ileal epithelial cells. L. fermentum also significantly reduced stress-induced increase in plasma corticosteronemia. In activated splenocytes, L. fermentum enhanced IFNγ secretion while it prevented IL-4 secretion. Finally, L. fermentum increased exploratory behavior. Conclusions & Inferences These results suggest that L. fermentum could provide a novel tool for the prevention and/or treatment of gastrointestinal disorders associated with altered IEB functions in the newborn. [ABSTRACT FROM AUTHOR]

Published
2017
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36. Low‐Dose Pesticide Mixture Induces Senescence in Normal Mesenchymal Stem Cells (MSC) and Promotes Tumorigenic Phenotype in Premalignant MSC

Author

Hochane, Mazene, Trichet, Valerie, Pecqueur, Claire, Avril, Pierre, Oliver, Lisa, Denis, Jerome, Brion, Regis, Amiaud, Jerome, Pineau, Alain, Naveilhan, Philippe, Heymann, Dominique, Vallette, François M., and Olivier, Christophe

Abstract

Humans are chronically exposed to multiple environmental pollutants such as pesticides with no significant evidence about the safety of such poly‐exposures. We exposed mesenchymal stem cells (MSC) to very low doses of mixture of seven pesticides frequently detected in food samples for 21 days in vitro. We observed a permanent phenotype modification with a specific induction of an oxidative stress‐related senescence. Pesticide mixture also induced a shift in MSC differentiation towards adipogenesis but did not initiate a tumorigenic transformation. In modified MSC in which a premalignant phenotype was induced, the exposure to pesticide mixture promoted tumorigenic phenotype both in vitro and in vivo after cell implantation, in all nude mice. Our results suggest that a common combination of pesticides can induce a premature ageing of adult MSC, and as such could accelerate age‐related diseases. Exposure to pesticide mixture may also promote the tumorigenic transformation in a predisposed stromal environment. Abstract Video Link: https://youtu.be/mfSVPTol-GkStemCells2017;35:800–811 Pesticide mixture at low doses induces progressive stress‐related senescence in normal mesenchymal stem cells (MSC), without modifying their death, in addition it favours their adipogenic differentiation rather than osteogenesis. In premalignant MSC, pesticide mixture promotes a tumorigenic phenotype in vitroand in vivo.

Published
2017
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37. Decreased choline acetyltransferase activity in nerve growth factor-transgenic mice during brain development

Author

Jacques Epelbaum, Brigitte Onteniente, Isabelle Neveu, Vantini G, Annie Faivre-Bauman, Naveilhan P, and Catherine Loudes

Subjects
Genetically modified mouse, medicine.medical_specialty, Aging, Central nervous system, Hypothalamus, Hippocampus, Gestational Age, Mice, Transgenic, Biology, Choline O-Acetyltransferase, chemistry.chemical_compound, Embryonic and Fetal Development, Mice, Prosencephalon, Reference Values, Internal medicine, medicine, Choline, Animals, Nerve Growth Factors, Cholinergic neuron, Cerebral Cortex, General Neuroscience, Embryo, Mammalian, Choline acetyltransferase, Nerve growth factor, medicine.anatomical_structure, Endocrinology, chemistry, Animals, Newborn, Acetylcholine, medicine.drug
Abstract

Activity of the synthetic enzyme for acetylcholine, choline acetyltransferase was investigated during development and in adult nerve growth factortransgenic mice. A conspicuous reduction of choline acetyltransferase activity was observed in the anterior brain of nerve growth factor-transgenic embryos from embryonic days 13 to 16 (E13 to E16). Choline acetyl-transferase activity levels subsequently resumed to normal levels, with the exception of a 15% increase in the adult hippocampus. Nerve growth factor contents followed a similar time-course and regional distribution in normal and nerve growth factor-transgenic animals and displayed significantly higher values from E 14 to the early postnatal period. Nerve growth factor contents were normal in the adult brain. In vitro experiments confirmed the involvement of nerve growth factor in the decrease of choline acetyltransferase activity levels observed in transgenic neurons during development. These results suggest a role for nerve growth factor in the initial phase of the phenotypic differentiation of cholinergic neurons. They show that nerve growth factor may, under specific developmental conditions, lead to a paradoxical down-regulation of choline acetyltransferase activity.

Published
1994

38. Synthesis of 1,25-dihydroxyvitamin D 3 by rat brain macrophages in vitro

Author

Neveu, I., Naveilhan, P., Menaa, C., Wion, Didier, Brachet, P., Garabédian, M., UMR643, Institut National de la Santé et de la Recherche Médicale (INSERM), Neuropathies du système nerveux entérique et pathologies digestives, implication des cellules gliales entériques, Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), wion, didier, and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes (UN)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1994
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39. LONGTERM CLINICAL RECOVERY IN PARKINSONIAN MONKEY RECIPIENTS OF CTLA4-IG TRANSGENIC PORCINE NEURAL PRECURSORS

Author

badin, Aron R., primary, Padoan, A., additional, Vadori, M., additional, Boldrin, M., additional, Cavicchioli, L., additional, De benedictis, G. M., additional, Fante, F., additional, Seveso, M., additional, Sgarabotto, D., additional, Jan, C., additional, Daguin, V., additional, Naveilhan, P., additional, Neveu, I., additional, Soulillou, J., additional, Vanhove, B., additional, Plat, M., additional, Bottè, F., additional, Eric, V., additional, Denaro, L., additional, Manara, R., additional, Zampieri, P., additional, Dʼavella, D., additional, Rubello, D., additional, Ancona, E., additional, Hantraye, P., additional, and Cozzi, E., additional

Published
2010
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