179 results on '"Navascués J"'
Search Results
2. Morphological Features of Pericycle Cells in Relation to Their Topographical Location in Onion Adventitious Roots
- Author
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Casero, P. J., Garcia-Sanchez, C., Lloret, P. G., and Navascues, J.
- Published
- 1989
3. The Effects of Removal of the Root Tip on Lateral Root Distribution in Adventitious Roots of Anion
- Author
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Lloret, P. G., Casero, P. J., Navascues, J., and Pulgarin, A.
- Published
- 1988
4. DIAGNOSTIC ACCURACY IN RESECABLE ESOPHAGEAL CANCER
- Author
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Andres Imaz, A, primary, Martí Gelonch, l, additional, Echeveste Varela, A, additional, Ariño Hervas, I, additional, Eizaguirre Letamendia, E, additional, Asensio Gallego, J I, additional, Murgoitio Lazcano, F J, additional, and Enríquez Navascués, J M, additional
- Published
- 2021
- Full Text
- View/download PDF
5. 4CPS-287 Impact of SARS-CoV-2 infection in acute myeloid leukaemia patients: experience of the Pethema registry
- Author
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Palanques Pastor, T, primary, Megias Vericat, J, additional, Martinez, P, additional, Cornago Navascués, J, additional, López Lorenzo, JL, additional, Rodríguez, G, additional, Cano, I, additional, Arnan Sangerman, M, additional, Poveda Andrés, JL, additional, and Montestinos, P, additional
- Published
- 2021
- Full Text
- View/download PDF
6. PLZF-RAR(alpha), NPM1-RAR(alpha), and other acute promyelocytic leukemia variants: the PETHEMA registry experience and systematic literature review
- Author
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Sobas, M. (Marta), Talarn-Forcadell, M.C. (Maria Carme), Martínez-Cuadron, D. (David), Escoda, L. (Lourdes), García-Pérez, M.J. (María J.), Mariz, J. (José), Mela-Osorio, M.J. (María J.), Fernandez, I. (Isolda), Alonso-Domínguez, J.M. (Juan M.), Cornago-Navascués, J. (Javier), Rodríguez-Macías, G. (Gabriela), Amutio, E. (Elena), Rodríguez-Medina, C. (Carlos), Esteve, J. (Jordi), Sokól, A. (Agniezska), Murciano-Carrillo, T. (Thais), Calasanz-Abinzano, M.J. (Maria Jose), Barrios, M. (M.), Barragán, E. (Eva), Sanz, M.A. (Miguel A.), and Montesinos, P. (Pau)
- Subjects
Characteristics ,Acute promyelocytic leukemia ,Systematic review ,Outcomes ,Variant ,neoplasms - Abstract
It has been suggested that 1-2% of acute promyelocytic leukemia (APL) patients present variant rearrangements of retinoic acid receptor alpha (RAR alpha) fusion gene, with the promyelocytic leukaemia zinc finger (PLZF)/RAR(alpha) being the most frequent. Resistance to all-trans-retinoic acid (ATRA) and arsenic trioxide (ATO) has been suggested in PLZF/RAR(alpha) and other variant APLs. Herein, we analyze the incidence, characteristics, and outcomes of variant APLs reported to the multinational PETHEMA (Programa para el Tratamiento de Hemopatias Malignas) registry, and we perform a systematic review in order to shed light on strategies to improve management of these extremely rare diseases. Of 2895 patients with genetically confirmed APL in the PETHEMA registry, 11 had variant APL (0.4%) (9 PLZF-RAR(alpha) and 2 NPM1-RAR(alpha)), 9 were men, with median age of 44.6 years (3 months to 76 years), median leucocytes (WBC) 16.8 x 10(9)/L, and frequent coagulopathy. Eight patients were treated with ATRA plus chemotherapy-based regimens, and 3 with chemotherapy-based. As compared to previous reports, complete remission and survival was slightly better in our cohort, with 73% complete remission (CR) and 73% survival despite a high relapse rate (43%). After analyzing our series and performing a comprehensive and critical review of the literature, strong recommendations on appropriate management of variant APL are not possible due to the low number and heterogeneity of patients reported so far.
- Published
- 2020
7. MICROGLIAL FUNCTIONS IN THE DEVELOPING RETINA: S14-01
- Author
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Marín-Teva, J. L., Sierra, A., Carrasco, M. D.C., Cuadros, M. A., Martín-Estebané, M., Martín-Oliva, D., Ferrer-Martín, R. M., Calvente, R., and Navascués, J.
- Published
- 2013
8. Proliferation of actively migrating ameboid microglia in the developing quail retina
- Author
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Marín-Teva, José L., Almendros, Antonio, Calvente, Ruth, Cuadros, Miguel A., and Navascués, J.
- Published
- 1999
- Full Text
- View/download PDF
9. An international multicentre prospective audit of elective rectal cancer surgery; operative approach versus outcome, including transanal total mesorectal excision (TaTME)
- Author
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Bhangu, Aneel, Minaya-Bravo, Ana María, Gallo, Gaetano, Glasbey, James C., Kamarajah, Sivesh, Pinkney, Thomas, El-Hussuna, Alaa, Battersby, Nick J., Buchs, Nicolas C., Buskens, Christianne, Chaudri, Sanjay, Frasson, Matteo, Glasbey, James, Morton, Dion, Negoi, Ionut, Nepogodiev, Dmitri, Pata, Francesco, Sánchez-Guillén, Luis, Singh, Baljit, Zmora, Oded, Perry, Rita, Magill, Laura, Altomare, Donato, Bemelman, Willem, Brown, Steven, Denost, Quentin, Knowles, Charles, Laurberg, Søren, Lefevre, Jérémie H., Möeslein, Gabriela, Pinkney, Tom, Vaizey, Carolynne, Bilali, S., Bilali, V., Salomon, M., Cillo, M., Estefania, D., Patron Uriburu, J., Ruiz, H., Farina, P., Carballo, F., Guckenheimer, S., Proud, D., Brouwer, R., Bui, A., Nguyen, B., Smart, P., Warwick, A., Theodore, J. E., Herbst, F., Birsan, T., Dauser, B., Ghaffari, S., Hartig, N., Stift, A., Argeny, S., Unger, L., Strouhal, R., Heuberger, A., Varabei, A., Lahodzich, N., Makhmudov, A., Selniahina, L., Feryn, T., Leupe, T., Maes, L., Reynvoet, E., Van Langenhove, K., Nachtergaele, M., Monami, B., Francart, D., Jehaes, C., Markiewicz, S., Weerts, J., Van Belle, K., Bomans, B., Cavenaile, V., Nijs, Y., Vertruyen, M., Pletinckx, P., Claeys, D., Defoort, B., Muysoms, F., Van Cleven, S., Lange, C., Vindevoghel, K., Wolthuis, A., Todorovic, M., Dabic, S., Kenjic, B., Lovric, S., Vidovic, J., Delibegovic, S., Mehmedovic, Z., Christiano, A., Lombardi, B., Marchiori, M., Tercioti, V., Dardanov, D., Petkov, P., Simonova, L., Yonkov, A., Zhivkov, E., Maslyankov, S., Pavlov, V., Sokolov, M., Todorov, G., Stoyanov, V., Batashki, I., Iarumov, N., Lozev, I., Moshev, B., Slavchev, M., Atanasov, B., Belev, N., Krstev, P., Penkov, R., Šantak, G., Ćosić, J., Previšić, A., Vukušić, L., Zukanović, G., Zelić, M., Kršul, D., Lekić Vitlov, V., Mendrila, D., Orhalmi, J., Dusek, T., Maly, O., Paral, J., Sotona, O., Skrovina, M., Bencurik, V., Machackova, M., Kala, Z., Farkašová, M., Grolich, T., Procházka, V., Hoch, J., Kocian, P., Martinek, L., Antos, F., Pruchova, V., Ceccotti, A., Madsbøll, T., Straarup, D., Uth Ovesen, A., Christensen, P., Bondeven, P., Edling, P., Elfeki, H., Alexandrovich Gameza, V., Michelsen Bach, S., Zheltiakova, I., Krarup, P. M., Krogh, A., Rolff, H. -C., Lykke, J., Juvik, A. F., Lóven, H. H. K., Marckmann, M., Osterkamp, J. T. F., Madsen, A. H., Worsøe, J., Ugianskis, A., Kjær, M. D., Youn Cho Lee, B., Khalid, A., Kristensen, M. H., El Sorogy, M., Elgeidie, A., Elhemaly, M., El Nakeeb, A., Elrefai, M., Shalaby, M., Emile, S., Omar, W., Sakr, A., Thabet, W., Awny, S., Metwally, I., Refky, B., Shams, N., Zuhdy, M., Lepistö, A., Keränen, I., Kivelä, A., Lehtonen, T., Siironen, P., Rautio, T., Ahonen-Siirtola, M., Klintrup, K., Paarnio, K., Takala, H., Hyöty, M., Haukijärvi, E., Kotaluoto, S. -M., Lehto, K., Tomminen, T., Huhtinen, H., Carpelan, A., Karvonen, J., Rantala, A., Varpe, P., Cotte, E., Francois, Y., Glehen, O., Passot, G., D'Alessandro, A., Chouillard, E., Etienne, J. C., Ghilles, E., Vinson-Bonnet, B., Germain, A., Ayav, A., Bresler, L., Chevalier, R., Denost, Q., Didailler, R., Rullier, E., Tiret, E., Chafai, N., Lefevre, J. H., Parc, Y., Sielezneff, I., Mege, D., Lakkis, Z., Barussaud, M., Krones, C., Bock, B., Webler, R., Baral, J., Lang, T., Münch, S., Pullig, F., Schön, M., Hinz, S., Becker, T., Möller, T., Richter, F., Schafmayer, C., Hardt, J., Kienle, P., Crescenti, F., Ahmad, M., Soleiman, Y., Papaconstantinou, I., Gklavas, A., Nastos, K., Theodosopoulos, T., Vezakis, A., Stamou, K., Saridaki, A., Xynos, E., Paraskakis, S., Zervakis, N., Skroubis, G., Amanatidis, T., Germanos, S., Maroulis, I., Papadopoulos, G., Dimitriou, N., Alexandrou, A., Felekouras, E., Griniatsos, J., Karavokyros, I., Papadopoulos, A., Chouliaras, C., Ioannidis, P., Katsounis, D., Kefalou, E., Katsoulis, I. E., Balalis, D., Korkolis, D. P., Manatakis, D., Tzovaras, G., Baloyiannis, I., Mamaloudis, I., Lázár, G., Ábraham, S., Paszt, A., Simonka, Z., Tóth, I., Zaránd, A., Baranyai, Z., Ferreira, G., Harsányi, L., Ónody, P., Banky, B., Burány, Ã., Lakatos, M., Marton, J., Solymosi, A., Besznyák, I., Bursics, A., Papp, G., Saftics, G., Svastics, I., Valsdottir, E., Atladottir, J., Jonsson, T., Moller, P., Sigurdsson, H., Gupta, S. K., Gupta, S., Kaul, N., Mohan, S., Sharma, G., Wani, R., Chowdri, N., Khan, M., Mehraj, A., Parray, F. Q., Coveney, A., Burke, J., Deasy, J., El-Masry, S., Mcnamara, D., Khan, M. F., Cahill, R., Faul, E., Mulsow, J., Shields, C., Kelly, M. E., Bass, G., Martin, S. T., O'Connell, R., Ryan, E., Connelly, T., Ahmad, G., Bukhari, W., Cooke, F., Zmora, O., Gold Deutch, R., Haim, N., Lavy, R., Moscovici, A., Shussman, N., Gefen, R., Marom, G., Pikarsky, A., Weiss, D., Avital, S., Hermann, N., Raguan, B., Slavin, M., White, I., Wasserberg, N., Arieli, H., Gurevich, N., Freund, M. R., Dorot, S., Edden, Y., Halfteck, G., Reissman, P., Pery, R., Tulchinsky, H., Weizman, A., Agresta, F., Curinga, R., Finotti, E., Savino, G., Verza, L. A., Asteria, C. R., Boccia, L., Pascariello, A., Tamini, N., Bugatti, A., Gianotti, L., Totis, M., Vincenti, L., Andriola, V., Giannini, I., Travaglio, E., Balestri, R., Buccianti, P., Roffi, N., Rossi, E., Urbani, L., Mellano, A., Cinquegrana, A., Lauretta, A., Belluco, C., Mistrangelo, M., Allaix, M. E., Arolfo, S., Morino, M., Testa, V., Delrio, P., Pace, U., Rega, D., Scala, D., Gallo, G., Clerico, G., Cornaglia, S., Realis Luc, A., Trompetto, M., Ugolini, G., Antonacci, N., Fabbri, S., Montroni, I., Zattoni, D., D'Urbano, C., Cornelli, A., Viti, M., Inama, M., Bacchion, M., Casaril, A., Impellizzeri, H., Moretto, G., Spinelli, A., Carvello, M., David, G., Di Candido, F., Sacchi, M., Frontali, A., Ceriani, V., Molteni, M., Rosati, R., Aleotti, F., Elmore, U., Lemma, M., Vignali, A., Scabini, S., Casoni Pattacini, G., Luzzi, A., Romairone, E., Marino, F., Lorusso, D., Pezzolla, F., Colombo, F., Baldi, C., Foschi, D., Sampietro, G., Sorrentino, L., Di Saverio, S., Birindelli, A., Segalini, E., Spacca, D., Romano, G. M., Belli, A., Bianco, F., De franciscis, S., Falato, A., Muratore, A., Marsanic, P., Grimaldi, S., Castaldo, N., Ciolli, M. G., Picarella, P., Porfidia, R., Tugnoli, G., Bondurri, A., Cavallo, D., Maffioli, A., Pertusati, A., Pulighe, F., Balestra, F., De Nisco, C., Podda, M., Opocher, E., Longhi, M., Mariani, N. M., Maroni, N., Pisani Ceretti, A., Galleano, R., Aonzo, P., Curletti, G., Reggiani, L., Marconi, M., Del Prete, L., Oldani, M., Pappalardo, R., Zaccone l, S., Scatizzi, M., Baraghini, M., Cantafio, S., Feroci, F., Giani, I., Tutino, R., Cocorullo, G., Gulotta, G., Licari, L., Salamone, G., Sileri, P., Saraceno, F., La Torre, F., Chirletti, P., Coletta, D., De Toma, G., Mingoli, A., Papandrea, M., De Luca, E., Sacco, R., Sammarco, G., Vescio, G., Tonini, V., Bianchini, S., Cervellera, M., Vaccari, S., Cracco, N., Barugola, G., Bertocchi, E., Rossini, R., Ruffo, G., Sartori, A., Clemente, N., De Luca, M., De Luca, A., Scaffidi, G., Lorenzon, L., Balducci, G., Bocchetti, T., Ferri, M., Mercantini, P., Pata, F., Bauce, S., Benevento, A., Bottini, C., Crapa, P. R., Rubbini, M., Anania, G., Carcoforo, P., Cavallesco, G., Feo, C., Yamamoto, T., Sivins, A., Ancans, G., Gerkis, S., Lunis, R., Pcolkins, A., Venskutonis, D., Bradulskis, S., Dainius, E., Subocius, A., Vencius, J., Zeromskas, P., Eismontas, V., Nutautiene, V., Simcikas, D., Tamosiunas, A., Svagzdys, S., Latkauskas, T., Lizdenis, P., Saladzinskas, Z., Tamelis, A., Dulskas, A., Kuliavas, J., Samalavicius, N. E., Poskus, T., Jotautas, V., Mikalauskas, S., Poskus, E., Strupas, K., Zakaria, A. D., Lah, N. N., Wong, M., Zain, W. Z., Zakaria, Z., Mazlan, L., Mohd Azman, Z. A., Sagap, I., Psaila, J., Andrejevic, P., Cini, C., Ellul, S., Pace, K., Ahallat, M., Hamid, M., Hrora, A., Majbar, M. A., Raiss, M., Westerduin, E., Bemelman, W., Buskens, C., Tanis, P., van der Sluis, P. C., Davids, P. H., Pronk, A., Schiphorst, A. H. W., Smakman, N., Zimmerman, D., Koeter, T., Stijns, J., van Loon, Y. -T., Vermaas, M., de Graaf, E., Doornebosch, P., van Hagen, P., van Ruler, O., Toorenvliet, B., Nonner, J., van den Berg, I., van Steensel, L., Vles, W., Melenhorst, J., Orsini, R., Visschers, R., Hoff, C., Blom, R., Marsman, H., Mulder, I., Cense, H., de Castro, S., Demirkiran, A., Hunfeld, M., van Geloven, A., de Groof, J., Hendriks, E., Leeuwenburg, M., van Oorschot, N., Wit, F., Rupert, C., Veldman, P., Keijzers, M., Konsten, J., Den Boer, F., Corver, M., Boerma, E. G., Koolen, L., Martens, M., Van Wijck, K., Ignjatovic, D., Breuer, R., Gurpreet, B., Oresland, T., Tetens Moe, T., Nesbakken, A., Flaaten Backe, I., Wik, T. -A., Radiya, K., Dehli, T., Gjessing, P., Norderval, S., Woll, K., Anwer, M., Qureshi, M. S., Qureshi, A. U., Billah, M., Jawad, M. Y., Raza, A., Urooj, N., Wang, X., Li, L., Jajtner, D., Gasinski, B., Kabiesz, W., Walega, P., Romaniszyn, M., Zawadzki, M., Czarnecki, R., Obuszko, Z., Rzaca, M., Sitarska, M., Silva, P., Duarte, A., Gonçalves, D., Morais, M., Rama, N., Nobre, J., Sales, I., Costa Pereira, J., Costa, S., Costa Pereira, C., Insua, C., Romero, I., Figueiredo, N., Cunha, J., Domingos, H., Vieira, P., Cunha, M., Americano, M., Amorim, E., Rachadell, J., Carvas, J. M., Armas, I., Fernandes, P., Pires, C., Reis, R., Martins, R., Dos Santos, M., Henriques, P., Oliveira, O., Duarte, M., Ferreira, L., Miranda, J., Vilela, N., Corte Real, J., Carlos, S., Frois Borges, M., Moniz Pereira, P., Simões, J., Silva-vaz, P., Bettencourt, V., Gouveia, A., Perez, H., Rainho, R., Bintintan, V., Ciuce, C., Dindelegan, G., Scurtu, R., Seicean, R., Cristian, D., Burcos, T., Grama, F., Mandi, D. M., Richiteanu, G., Miron, A., Calu, V., Enciu, O., Nadragea, M., Parvuletu, R., Mogoanta, S. S., Crafciuc, A., Paitici, S., Negoi, I., Beuran, M., Ciubotaru, C., Prodan, A., Vartic, M., Tomulescu, V., Copaescu, C., Yanishev, A., Abelevich, A., Kokobelyan, A., Lebedeva, M., Luzan, R., Pozdnyakov, A., Cherdancev, D., Mahotin, D., Nesytykh, A., Samsonyuk, V., Pravosudov, I., Ivlev, D., Karachun, A., Lebedev, K., Samsonov, D., Aiupov, R., Feoktistov, D., Garipov, M., Suleymanov, N., Tarasov, N., Rasulov, A., Dzhumabaev, H., Mamedli, Z., Bedzhanyan, A., Popov, D., Sednev, A., Klimenko, A., Semenov, A., Vasilyev, S., Khazov, A., Khanevich, M., Khrykov, G., Katorkin, S., Andreev, P., Chernov, A., Davidova, O., Zhuravlev, A., Achkasov, S., Shakhmatov, D., Shelygin, Y., Sushkov, O., Vardanyan, A., Ilkanich, A., Barbashinov, N., Darvin, V., Onishchenko, S., Voronin, Y., Krivokapić, Z., Barišić, G., Dimitrijević, I., Marković, V., Sekulić, A., Stanojevic, G., Brankovic, B., Nestorovic, M., Pecic, V., Petrovic, D., Kostic, I., Aleksic, A., Dabic, D., Maric, B., Perunicic, V., Radovanovic, Z., Djuric, M., Lukic, D., Radovanovic, D., Cuk, V., Juloski, J., Kenic, M., Krdzic, I., Ngu, J. C., Y. Y., Ng, Teo, N., Korcek, J., Lazorisak, A., Rems, M., Ramovš Trampuš, Å ., Tomazic, A., Grosek, J., Kosir, J., Norcic, G., Vigorita, V., Caceres, N., Casal, E., Ruano, A., Trostchansky, I., Golda, T., Galvez Saldaña, A., Kreisler Moreno, E., Lopez Dominguez, J., Vila Tura, M., Labarga, F., Galvez, P., Maderuelo, V., Suero, C., Bargallo, J., Cayetano, L., Lamas, S., Silva, M. C., Bernal-Sprekelsen, J. C., Gómez, R., Jareño, S., Ríos, A., Vercher, D., García-González, J. -M., Cervera-Aldama, J., Ramos-Prada, J., Santamaría-Olabarrieta, M., Borda, N., Enrriquez-Navascués, J. M., Saralegui, Y., Calero-Lillo, A., Aznar-Puig, S., López-Lara, M. A., Muñoz-Collado, S., Valverde-Sintas, J., Menendez, P., Leon, C., Truan, N., Baldonedo, R., Fernández-Martínez, D., Otero, J., Solar-García, L., Turrado-Rodriguez, V., de Lacy Oliver, F., Lacy Fortuny, A. M., Martin Perez, B., Otero Piñeiro, A. M., Paredes, J., Fernandez, F., Ladra, M. J., Paulos, A., Prieto, D., Beltrán de Heredia, J. P., Blanco Antona, F., de Andrés Asenjo, B., Ferreras García, C., Romero de Diego, A., Cordoba Diaz de Laspra, E., Echazarreta Gallego, E., Elia Guedea, M., Escola, D., Martinez, S., Primo Romaguera, V., Parreño, R., Pastor, L., Rosell, E., Lozoya Trujillo, R., Alós Company, R., Ruiz Carmona, M. D., Solana Bueno, A., Salvans Ruiz, S., Alonso Gonçalves, S., Jiménez- Toscano, M., Pascual Damieta, M., Pera Roman, M., Pellicer-Franco, E. M., Garcia-Marin, J. A., Mengual-Ballester, M., Soria-Aledo, V., Valero-Navarro, G., Vicente-Ruiz, M., Garcia-Zamora, C., Gonzalez-Gil, A., Montoya-Tabares, M. J., Paredes-Quiles, M., Die Trill, J., Abadia, P., Moreno, I., Pina, J. D., Ramos Rubio, D., Escartin, J., Blas, J. L., Fernando, J., Ferrer, R., Garcia Egea, J., Pros, I., Martinez, W., Rius, J., Socías, M., Sabia, D., Castellvi Valls, J., Gonzalez Santin, V., Mompart Garcia, S., Viso Pons, L., Julià, D., Codina-Cazador, A., Farrés, R., Gómez, N., Planellas, P., Cuadrado, M., Camps, I., Rufas, M., Escoll, J., Fermiñán, A., Muriel, P., Sierra, E., Alvarez-Laso, C., Lora, P., Padin, H., Garcia-Septiem, J., Bustamante, C., Jimenez, V., Jimenez-Miramon, J., Ramos, J. L., Gallardo, A. B., Benito, P., Colao, L., Galindo, P., Garcia, C., Forero-Torres, A., Alonso Poza, A., Dieguez Fernandez, B., Gilsanz Martin, C., Hernandez Garcia, M., Rojo López, J. A., Gil López, J. M., González Zunzárren, M., Martínez Alegre, J., Zorrilla Matilla, L. P., Navarro-Sánchez, A., Alcalá Serrano, F. J., López-Fernández, J., Montesdeoca Cabrera, D., Alvarez-Gallego, M., Guevara, J., Pascual Miguelañez, I., Rubio-Perez, I., Gomez Ruiz, M., Alonso Martín, J., Cagigas Fernández, C., Castillo Diego, J., Pando, J. A., Maristany, C., Muñoz-Duyos, A., Rada-Palomino, A., Vargas-Pierola, H., Peña Ros, E., Benavides Buleje, J. A., Muñoz Camarena, J. M., Parra Baños, P. A., Ramirez Faraco, M., Arenal, J. J., Citores, M. A., Marcos, J. L., Sánchez, J., Tinoco, C., García Flórez, L. J., Arias Pacheco, R. D., Mínguez Ruiz, G., Gutiérrez Corral, N., Rodríguez Infante, A., Carrillo López, M. J., Carrasco Prats, M. M., Lage Laredo, A., Martínez Manzano, Ã., Rodríguez García, P., Segura-Sampedro, J. J., Alonso-Hernández, N., Fernandez Isart, M., Gamundi Cuesta, M., Ochogavia Segui, A., Ibañez, N., Abrisqueta, J., Lujan, J., Gómez Pérez, R., Corrales Valero, E., Monje Salazar, C., Sanchiz Cardenas, E., Soler Humanes, R., Jimenez-Rodriguez, R. M., De la Portilla, F., Diaz Pavon, J. M., Garcia Cabrera, A. M., Reyes Diaz, M. L., Espin, E., Marinello, F., Martí, M., Sanchez, J. L., Vallribera, F., Orts Mico, F. 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S., Crafciuc, A., Paitici, S., Negoi, I., Beuran, M., Ciubotaru, C., Prodan, A., Vartic, M., Tomulescu, V., Copaescu, C., Yanishev, A., Abelevich, A., Kokobelyan, A., Lebedeva, M., Luzan, R., Pozdnyakov, A., Cherdancev, D., Mahotin, D., Nesytykh, A., Samsonyuk, V., Pravosudov, I., Ivlev, D., Karachun, A., Lebedev, K., Samsonov, D., Aiupov, R., Feoktistov, D., Garipov, M., Suleymanov, N., Tarasov, N., Rasulov, A., Dzhumabaev, H., Mamedli, Z., Bedzhanyan, A., Popov, D., Sednev, A., Klimenko, A., Semenov, A., Vasilyev, S., Khazov, A., Khanevich, M., Khrykov, G., Katorkin, S., Andreev, P., Chernov, A., Davidova, O., Zhuravlev, A., Achkasov, S., Shakhmatov, D., Shelygin, Y., Sushkov, O., Vardanyan, A., Ilkanich, A., Barbashinov, N., Darvin, V., Onishchenko, S., Voronin, Y., Krivokapić, Z., Barišić, G., Dimitrijević, I., Marković, V., Sekulić, A., Stanojevic, G., Brankovic, B., Nestorovic, M., Pecic, V., Petrovic, D., Kostic, I., Aleksic, A., Dabic, D., Maric, B., Perunicic, V., Radovanovic, Z., Djuric, M., Lukic, D., Radovanovic, D., Cuk, V., Juloski, J., Kenic, M., Krdzic, I., Ngu, J. 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P., Blanco Antona, F., de Andrés Asenjo, B., Ferreras García, C., Romero de Diego, A., Cordoba Diaz de Laspra, E., Echazarreta Gallego, E., Elia Guedea, M., Escola, D., Martinez, S., Primo Romaguera, V., Parreño, R., Pastor, L., Rosell, E., Lozoya Trujillo, R., Alós Company, R., Ruiz Carmona, M. D., Solana Bueno, A., Salvans Ruiz, S., Alonso Gonçalves, S., Jiménez- Toscano, M., Pascual Damieta, M., Pera Roman, M., Pellicer-Franco, E. M., Garcia-Marin, J. A., Mengual-Ballester, M., Soria-Aledo, V., Valero-Navarro, G., Vicente-Ruiz, M., Garcia-Zamora, C., Gonzalez-Gil, A., Montoya-Tabares, M. J., Paredes-Quiles, M., Die Trill, J., Abadia, P., Moreno, I., Pina, J. D., Ramos Rubio, D., Escartin, J., Blas, J. L., Fernando, J., Ferrer, R., Garcia Egea, J., Pros, I., Martinez, W., Rius, J., Socías, M., Sabia, D., Castellvi Valls, J., Gonzalez Santin, V., Mompart Garcia, S., Viso Pons, L., Julià, D., Codina-Cazador, A., Farrés, R., Gómez, N., Planellas, P., Cuadrado, M., Camps, I., Rufas, M., Escoll, J., Fermiñán, A., Muriel, P., Sierra, E., Alvarez-Laso, C., Lora, P., Padin, H., Garcia-Septiem, J., Bustamante, C., Jimenez, V., Jimenez-Miramon, J., Ramos, J. L., Gallardo, A. B., Benito, P., Colao, L., Galindo, P., Garcia, C., Forero-Torres, A., Alonso Poza, A., Dieguez Fernandez, B., Gilsanz Martin, C., Hernandez Garcia, M., Rojo López, J. A., Gil López, J. M., González Zunzárren, M., Martínez Alegre, J., Zorrilla Matilla, L. P., Navarro-Sánchez, A., Alcalá Serrano, F. J., López-Fernández, J., Montesdeoca Cabrera, D., Alvarez-Gallego, M., Guevara, J., Pascual Miguelañez, I., Rubio-Perez, I., Gomez Ruiz, M., Alonso Martín, J., Cagigas Fernández, C., Castillo Diego, J., Pando, J. A., Maristany, C., Muñoz-Duyos, A., Rada-Palomino, A., Vargas-Pierola, H., Peña Ros, E., Benavides Buleje, J. A., Muñoz Camarena, J. M., Parra Baños, P. A., Ramirez Faraco, M., Arenal, J. J., Citores, M. A., Marcos, J. L., Sánchez, J., Tinoco, C., García Flórez, L. J., Arias Pacheco, R. D., Mínguez Ruiz, G., Gutiérrez Corral, N., Rodríguez Infante, A., Carrillo López, M. J., Carrasco Prats, M. M., Lage Laredo, A., Martínez Manzano, Ã ., Rodríguez García, P., Segura-Sampedro, J. J., Alonso-Hernández, N., Fernandez Isart, M., Gamundi Cuesta, M., Ochogavia Segui, A., Ibañez, N., Abrisqueta, J., Lujan, J., Gómez Pérez, R., Corrales Valero, E., Monje Salazar, C., Sanchiz Cardenas, E., Soler Humanes, R., Jimenez-Rodriguez, R. M., De la Portilla, F., Diaz Pavon, J. M., Garcia Cabrera, A. M., Reyes Diaz, M. L., Espin, E., Marinello, F., Martí, M., Sanchez, J. L., Vallribera, F., Orts Mico, F. J., Ortin Navarro, M., Perez Climent, M., Serra Diaz, C., Millan, M., Caro, A., Escuder, J., Espina, B., Feliu, F., Climent Aira, A., Estévez Diz, A., Moreno Asencio, M. T., Varela Mato, A., Vázquez Bouzán, R., Minaya-Bravo, A. M., Diez-Alonso, M. M., Villeta-Plaza, R., Guadalajara, H., Alías, D., García Olmo, D., Pastor, C., Valverde, I., Sanchez Romero, A., Gardea, A., Gil Santos, M., Nimmersgern, T., Serrano Paz, P., Romero-Simó, M., Blasco-Segura, T., Caravaca-García, I., Costa-Navarro, D., Zarco-Pleguezuelos, A., Sánchez-Guillén, L., Flor-Lorente, B., Frasson, M., García-Granero, Ã ., García-Granero, E., Arencibia, B., Alonso, J., Febles, G., Nogués, E. M., Roque, C., Segelman, J., Nygren, J., Nestler, G., Abraham-Nordling, M., Egenvall, M., Myrelid, P., Jung, B., Loftås, P., Lydrup, M. -L., Azahr, N., Buchwald, P., Mangell, P., Syk, I., Nikberg, M., Carlander, J., Chabok, A., Smedh, K., Tiselius, C., Haapaniemi, S., Benckert, A., Adamina, M., Freil-Lanter, C., Gingert, C., Müller, P., Schäfli, J., Regusci, L., Brenna, M., Fasolini, F., Misteli, H., Kirchhoff, P., Oertli, D., Hahnloser, D., Clerc, D., Hübner, M., Ris, F., Buchs, N. C., Chevallay, M., Morel, P., Schiltz, B., Wang, J. Y., Su, W. -C., Huang, C. -W., Ma, C. -J., Tsai, H. -L., Bugra, D., Agalar, F., Baloglu, H., Basoglu, I., Okkabaz, N., Binboga, E., Biricik, A., Celik, A., Yavuz, E., Canda, A. E., Agalar, C., Fuzun, M., Sokmen, S., Terzi, C., Isik, A., Karip, B., Bilgili, A. C., Leventoğlu, S., Aytac, B., Küçükdiler, E., Yıldız, A., Yuksel, O., Sinan, H., Hancerliogullari, O., Kaymak, S., Kozak, O., Ozer, M. T., Sarici, I. S., Akca, O., Kalayci, M. U., Kara, Y., Agcaoglu, O., Balik, E., Bayram, O., Özbalcı, G. S., Özkan, B. B., Karabacak, U., Sungurtekin, U., Ozgen, U., Demirbas, S., Öztürk, E., Isik, O., Yilmazlar, T., Colak, E., Karagul, S., Kinas, V., Fearnhead, N., Lord, I., Stewart, P., Zammit, M., Arnold, S., Battersby, N. J., Broadhurst, J., Moran, S., Seretis, F., Shabbir, J., Jones, C., Kynaston, J., Vimalachandran, D., Blower, E., Mcfaul, C., Mcwhirter, D., Pilkington, J., Wilson, T., Chowdhary, M., Stubbs, B., Abdalkoddus, M., Lai, C., Thavanesan, N., Yao, C., Agarwal, T., Dindyal, S., Hill, R. M. C., Reade, S., Slesser, A., Paterson, H., Balfour, A., Boland, M., Geraghty, A., O'Kelly, J., Patel, P., Tezas, S., Yahia, S., Jadhav, V., Marimuthu, K., Narayanan, A., Piramanayagam, B., Bradley, N., Buchanan, F., Paul, K., Singh, J., Thomson, K., Korsgen, S., Bedford, M., Lee, K., Leong, K., Mcarthur, D., Bhangu, A., Malik, S., Mohamed, I., Cunha, P., Pilavas, A., Reddy, A., Ahmed, S., Ahmed, A., Voll, J., Velchuru, V., Lal, R., Mirshekar-Syahkal, B., Kassai, M., Aleem, M., Keogh-Bootland, S., Sarmah, P., Brown, S., Keegan, R., Kelkar, A., Sen, P., Oliveira-Cunha, M., Chaudhri, S., Fares, R., Singh, B., Thomas, W. M., Aslam, M. I., Boyle, K., Hemingway, D., Miller, A., Norwood, M., Gurjar, S., Al-Saeedi, M., Anandan, L., Sudlow, A., Zampitis, N., Malik, K., Bogdan, M., Smart, C., Iqbal, M. R., Bailey, S., Lawes, D., Omar, G., Tamhane, R., Evans, M., Ather, S., Lim, J., Nageswaran, H., Taylor, G., Hunt, L., Nicholls, J., Shaikh, I., Muscara, F., O'Brien, J., Photi, E., Stearns, A., Meylemans, D., Cunningham, C., Hompes, R., Tennakoon, A., Kumarasinghe, N., Rao, M., Upanishad, I., Khan, J., Ahmad, N., Shweejawee, Z., Stefan, S., Smart, N., Daniels, I., Gregoir, T., Longstaff, L., Mcdermott, F., Varcada, M., Drami, I., Gala, T., Moggia, E., Ratnatunga, K., Harries, R., Hayes, J., Williams, G., Raymond, T., Bronder, C., Davies, E., Hawkin, P., Ryska, O., Ayral, K., Beveridge, A., Bhowmik, A., Gill, M., Simpson, R., Schofield, A., Mcardle, K., Parmar, M., Williamson, M., Burton, H., Courtney, E., Grant, C., Saracino, A., Newton, K., Epstein, J., Branagan, G., Bignell, M., Symankewicz, M., Zaman, S., Mankotia, R., Siddiqui, Z., Torrance, A., Artioukh, D., Eggleston, M., Gokul, K., Selwyn, D., Warusavitarne, J., Chandrasinghe, P., Grainger, J., Leo, C. A., Vaizey, C. J., Harris, G., Levy, B., Skull, A., Thaha, M., Garg, A., Patel, H., Ramsanahie, A., Mondragon-Pritchard, M., Cuinas Leon, K., Shukla, A., Brewer, H., Fitzgerald, J., Kho, H., Torkington, J., Tate, S., Wheat, J., Smolarek, S., Platt, E., Rossi, B., Tham, J. C., Knight, J., Richardson, J., Tzivanakis, A., Gregori, M., Ashraf, M. A., Atif, M., Santos, J., Saffaf, N., Canning, L., Chandratreya, N., Bowen, M., Graham, B., Hamad, Y., Kaubrys, M., Chaudhry, Z. U., Bhan, C., Mukhtar, H., Oshowo, A., Wilson, J., Gouvas, N., Nicol, D., Pandey, S., Zilvetti, M., Sharma, A., Fatayer, T., Mothe, S., Rahman, M., Curtis, N., Allison, A., Dalton, R., Francis, N., Ockrim, J., Psaras, G., Dudarovaska, H., Marharint, T., Mostovoy, E., Voloshin, S., Kolesnik, O., Makhmudov, D., Altinel, Y., Iqbal, A., Cunningham, L., Go, K., Tan, S., R. Tutino, G. salamone, L. Licari, G. Cocorullo, G. Gulotta, AGEM - Digestive immunity, AGEM - Re-generation and cancer of the digestive system, and CCA - Cancer Treatment and Quality of Life
- Subjects
Male ,Laparoscopic surgery ,medicine.medical_specialty ,Colorectal cancer ,medicine.medical_treatment ,Operative Time ,Anastomosis ,Malignancy ,rectal cancer ,laparoscopic surgery ,TME ,transanal TME ,TaTME ,robotic surgery ,randomized clinical-trial ,short-term outcomes ,laparoscopic-assisted resection ,pathological outcomes ,anastomotic leakage ,initial-experience ,risk ,NO ,03 medical and health sciences ,Postoperative Complications ,0302 clinical medicine ,Robotic Surgical Procedures ,Humans ,Medicine ,Robotic surgery ,Prospective Studies ,Rectal cancer ,Gastroenterology ,Aged ,Transanal Endoscopic Surgery ,ta3126 ,Medical Audit ,Univariate analysis ,Proctectomy ,Rectal Neoplasms ,business.industry ,Rectum ,Margins of Excision ,Middle Aged ,medicine.disease ,Total mesorectal excision ,Surgery ,Treatment Outcome ,Elective Surgical Procedures ,030220 oncology & carcinogenesis ,Rectal cancer surgery ,Female ,Laparoscopy ,030211 gastroenterology & hepatology ,business - Abstract
Introduction: Transanal total mesorectal excision (TaTME) has rapidly emerged as a novel approach for rectal cancer surgery. Safety profiles are still emerging and more comparative data is urgently needed. This study aimed to compare indications and short-term outcomes of TaTME, open, laparoscopic, and robotic TME internationally. Methods: A pre-planned analysis of the European Society of Coloproctology (ESCP) 2017 audit was performed. Patients undergoing elective total mesorectal excision (TME) for malignancy between 1 January 2017 and 15 March 2017 by any operative approach were included. The primary outcome measure was anastomotic leak. Results: Of 2579 included patients, 76.2% (1966/2579) underwent TME with restorative anastomosis of which 19.9% (312/1966) had a minimally invasive approach (laparoscopic or robotic) which included a transanal component (TaTME). Overall, 9.0% (175/1951, 15 missing outcome data) of patients suffered an anastomotic leak. On univariate analysis both laparoscopic TaTME (OR 1.61, 1.02–2.48, P=0.04) and robotic TaTME (OR 3.05, 1.10–7.34, P=0.02) were associated with a higher risk of anastomotic leak than non-transanal laparoscopic TME. However this association was lost in the mixed-effects model controlling for patient and disease factors (OR 1.23, 0.77–1.97, P=0.39 and OR 2.11, 0.79–5.62, P=0.14 respectively), whilst low rectal anastomosis (OR 2.72, 1.55–4.77, P 
- Published
- 2018
10. Evaluating the incidence of pathological complete response in current international rectal cancer practice: the barriers to widespread safe deferral of surgery
- Author
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Battersby, Nick, Glasbey, James C., Neary, Peter, Negoi, Ionut, Kamarajah, Sivesh, Sgro, Alessandro, Bhangu, Aneel, Pinkney, Thomas, Frasson, Matteo, El-Hussuna, Alaa, Battersby, Nick J., Buchs, Nicolas C., Buskens, Christianne, Chaudri, Sanjay, Gallo, Gaetano, Glasbey, James, Minaya-Bravo, Ana María, Morton, Dion, Nepogodiev, Dmitri, Pata, Francesco, Sánchez-Guillén, Luis, Singh, Baljit, Zmora, Oded, Perry, Rita, Magill, Laura, Altomare, Donato, Bemelman, Willem, Brown, Steven, Denost, Quentin, Knowles, Charles, Laurberg, Søren, Lefevre, Jérémie H., Möeslein, Gabriela, Pinkney, Tom, Vaizey, Carolynne, Bilali, S., Bilali, V., Salomon, M., Cillo, M., Estefania, D., Patron Uriburu, J., Ruiz, H., Farina, P., Carballo, F., Guckenheimer, S., Proud, D., Brouwer, R., Bui, A., Nguyen, B., Smart, P., Warwick, A., Theodore, J. 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Tutino, G. Salamone, L. Licari, G. Cocorullo, and G. Gulotta
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Male ,Colorectal cancer ,deferral of surgery ,neoadjuvant therapy ,pathology ,radiology ,rectal cancer ,Rectal surgery ,surgical oncology ,Gastroenterology ,0302 clinical medicine ,Prospective Studies ,Prospective cohort study ,Complete response ,Medical Audit ,integumentary system ,Incidence (epidemiology) ,Incidence ,Remission Induction ,Middle Aged ,Magnetic Resonance Imaging ,Europe ,Treatment Outcome ,Response Evaluation Criteria in Solid Tumors ,030220 oncology & carcinogenesis ,Preoperative Period ,030211 gastroenterology & hepatology ,Female ,Adult ,medicine.medical_specialty ,NO ,03 medical and health sciences ,medicine ,Humans ,Intensive care medicine ,Deferral ,Pathological ,Aged ,Neoplasm Staging ,ta3126 ,business.industry ,Rectal Neoplasms ,Reproducibility of Results ,Chemoradiotherapy, Adjuvant ,medicine.disease ,Rectal Neoplasms/diagnostic imaging ,Neoplasm Staging/methods ,business - Abstract
INTRODUCTION: The mainstay of management for locally advanced rectal cancer is chemoradiotherapy followed by surgical resection. Following chemoradiotherapy, a complete response may be detected clinically and radiologically (cCR) prior to surgery or pathologically after surgery (pCR). We aim to report the overall complete pathological response (pCR) rate and the reliability of detecting a cCR by conventional pre-operative imaging.METHODS: A pre-planned analysis of the European Society of Coloproctology (ESCP) 2017 audit was performed. Patients treated by elective rectal resection were included. A pCR was defined as a ypT0 N0 EMVI negative primary tumour; a partial response represented any regression from baseline staging following chemoradiotherapy. The primary endpoint was the pCR rate. The secondary endpoint was agreement between post-treatment MRI restaging (yMRI) and final pathological staging.RESULTS: Of 2572 patients undergoing rectal cancer surgery in 277 participating centres across 44 countries, 673 (26.2%) underwent chemoradiotherapy and surgery. The pCR rate was 10.3% (67/649), with a partial response in 35.9% (233/649) patients. Comparison of AJCC stage determined by post-treatment yMRI with final pathology showed understaging in 13% (55/429) and overstaging in 34% (148/429). Agreement between yMRI and final pathology for T-stage, N-stage, or AJCC status were each graded as 'fair' only (n = 429, Kappa 0.25, 0.26 and 0.35 respectively).CONCLUSION: The reported pCR rate of 10% highlights the potential for non-operative management in selected cases. The limited strength of agreement between basic conventional post-chemoradiotherapy imaging assessment techniques and pathology suggest alternative markers of response should be considered, in the context of controlled clinical trials.
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- 2018
11. Intranuclear functions of SUMO-1 in neuronal differentiation and stress response: 81
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Navascués, J., Bengoechea, R., Berciano, M. T., and Lafarga, M.
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- 2009
12. Poly(ADP-Ribose) Polymerase-1 inhibition potentiates cell death and phosphorylation of DNA damage response proteins in oxidative stressed retinal cells
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Martín-Guerrero, S.M., Casado, P., Muñoz-Gámez, J.A., Carrasco, M.C., Navascués, J., Cuadros, Miguel A., López-Giménez, Juan F., Cutillas, P.R., Martín-Oliva, David, Martín-Guerrero, S.M., Casado, P., Muñoz-Gámez, J.A., Carrasco, M.C., Navascués, J., Cuadros, Miguel A., López-Giménez, Juan F., Cutillas, P.R., and Martín-Oliva, David
- Abstract
Oxidative stress (OxS) is involved in the development of cell injures occurring in retinal diseases while Poly(ADP-ribose) Polymerase-1 (PARP-1) is a key protein involved in the repair of the DNA damage caused by OxS. Inhibition of PARP-1 activity with the pharmacological inhibitor PJ34 in mouse retinal explants subjected to HO-induced oxidative damage resulted in an increase of apoptotic cells. Reduction of cell growth was also observed in the mouse cone like cell line 661 W in the presence of PJ34 under OxS conditions. Mass spectrometry-based phosphoproteomics analysis performed in 661 W cells determined that OxS induced significant changes in the phosphorylation in 1807 of the 8131 peptides initially detected. Blockade of PARP-1 activity after the oxidative treatment additionally increased the phosphorylation of multiple proteins, many of them at SQ motifs and related to the DNA-damage response (DDR). These motifs are substrates of the kinases ATM/ATR, which play a central role in DDR. Western blot analysis confirmed that the ATM/ATR activity measured and the phosphorylation at SQ motifs of ATM/ATR substrates was augmented when PARP-1 activity was inhibited under OxS conditions, in 661 W cells. Phosphorylation of ATM/ATR substrates, including the phosphorylation of the histone H2AX were also induced in organotypic cultures of retinal explants subjected to PARP-1 inhibition during exposure to OxS. In conclusion, inhibition of PARP-1 increased the phosphorylation and hence the activation of several proteins involved in the response to DNA damage, like the ATM protein kinase. This finally resulted in an augmented injury in mouse retinal cells suffering from OxS. Therefore, the inhibition of PARP-1 activity may have a negative outcome in the treatment of retinal diseases in which OxS is involved.
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- 2019
13. Macrophage-like cells invading the suboptic necrotic centres of the avian embryo diencephalon originate from haemopoietic precursors
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Martín-Partido, G., Cuadros, M. A., Martin, C., Coltey, P., and Navascués, J.
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- 1991
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14. Morphological changes and transversal growth kinetics along the apical meristem in the pericycle cell types of the onion adventitious root
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García-Sánchez, C., Casero, P. J., Lloret, P. G., and Navascués, J.
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- 1991
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15. Intrapelvic placement of a breast implant to allow deferred ileoanal pouch anastomosis after emergency proctocolectomy
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Enríquez-Navascués, J. M., Alkorta, M., Borda, N., and Placer, C.
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- 2014
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16. Macrophage-like cells in the presumptive optic pathways in the floor of the diencephalon of the chick embryo
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MartÍn-Partido, G. and NavascuÉs, J.
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- 1990
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17. The impact of conversion on the risk of major complication following laparoscopic colonic surgery: an international, multicentre prospective audit
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van der Pool, Anne, Rawlings, Alexandra, Sánchez-Guillén, Luis, Kuiper, Sara, Negoi, Ionut, Buchs, Nicolas, El-Hussuna, Alaa, Battersby, Nick J., Buchs, Nicolas C., Buskens, Christianne, Chaudri, Sanjay, Frasson, Matteo, Gallo, Gaetano, Minaya-Bravo, Ana María, Morton, Dion, Nepogodiev, Dmitri, Pata, Francesco, Singh, Baljit, Zmora, Oded, Perry, Rita, Magill, Laura, Altomare, Donato, Bemelman, Willem, Brown, Steven, Denost, Christianne Buskens Quentin, Knowles, Charles, Laurberg, Søren, Lefevre, Jérémie H., Möeslein, Gabriela, Pinkney, Tom, Vaizey, Carolynne, Bilali, S., Bilali, V., Salomon, M., Cillo, M., Estefania, D., Patron Uriburu, J., Ruiz, H., Farina, P., Carballo, F., Guckenheimer, S., Proud, D., Brouwer, R., Bui, A., Nguyen, B., Smart, P., Warwick, A., Theodore, J. 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M., Krogh, A., Rolff, H. -C., Lykke, J., Juvik, A. F., Lóven, H. H. K., Marckmann, M., Osterkamp, J. T. F., Madsen, A. H., Worsøe, J., Ugianskis, A., Kjær, M. D., Youn Cho Lee, B., Khalid, A., Kristensen, M. H., El Sorogy, M., Elgeidie, A., Elhemaly, M., El Nakeeb, A., Elrefai, M., Shalaby, M., Emile, S., Omar, W., Sakr, A., Thabet, W., Awny, S., Metwally, I., Refky, B., Shams, N., Zuhdy, M., Lepistö, A., Keränen, I., Kivelä, A., Lehtonen, T., Siironen, P., Rautio, T., Ahonen-Siirtola, M., Klintrup, K., Paarnio, K., Takala, H., Hyöty, M., Haukijärvi, E., Kotaluoto, S. -M., Lehto, K., Tomminen, T., Huhtinen, H., Carpelan, A., Karvonen, J., Rantala, A., Varpe, P., Cotte, E., Francois, Y., Glehen, O., Passot, G., D'Alessandro, A., Chouillard, E., Etienne, J. C., Ghilles, E., Vinson-Bonnet, B., Germain, A., Ayav, A., Bresler, L., Chevalier, R., Denost, Q., Didailler, R., Rullier, E., Tiret, E., Chafai, N., Lefevre, J. 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Simcikas, D, Tamosiunas, A, Svagzdys, S, Latkauskas, T, Lizdenis, P, Saladzinskas, Z, Tamelis, A, Dulskas, A, Kuliavas, J, Samalavicius, N, Poskus, T, Jotautas, V, Mikalauskas, S, Poskus, E, Strupas, K, Zakaria, A, Lah, N, Wong, M, Zain, W, Zakaria, Z, Mazlan, L, Mohd Azman, Z, Sagap, I, Psaila, J, Andrejevic, P, Cini, C, Ellul, S, Pace, K, Ahallat, M, Hamid, M, Hrora, A, Majbar, M, Raiss, M, Westerduin, E, Tanis, P, van der Sluis, P, Davids, P, Pronk, A, Schiphorst, A, Smakman, N, Zimmerman, D, Koeter, T, Stijns, J, van Loon, Y, Vermaas, M, de Graaf, E, Doornebosch, P, van Hagen, P, van Ruler, O, Toorenvliet, B, Nonner, J, van den Berg, I, van Steensel, L, Vles, W, Melenhorst, J, Orsini, R, Visschers, R, Hoff, C, Blom, R, Marsman, H, Mulder, I, Cense, H, de Castro, S, Demirkiran, A, Hunfeld, M, van Geloven, A, de Groof, J, Hendriks, E, Leeuwenburg, M, van Oorschot, N, Wit, F, Rupert, C, Veldman, P, Keijzers, M, Konsten, J, Den Boer, F, Corver, M, Boerma, E, Koolen, L, Martens, M, Van Wijck, K, Ignjatovic, D, Breuer, R, Gurpreet, B, Oresland, T, Tetens Moe, T, Nesbakken, A, Flaaten Backe, I, Wik, T, Radiya, K, Dehli, T, Gjessing, P, Norderval, S, Woll, K, Anwer, M, Qureshi, M, Qureshi, A, Billah, M, Jawad, M, Raza, A, Urooj, N, Wang, X, Li, L, Jajtner, D, Gasinski, B, Kabiesz, W, Walega, P, Romaniszyn, M, Zawadzki, M, Czarnecki, R, Obuszko, Z, Rzaca, M, Sitarska, M, Silva, P, Duarte, A, Goncalves, D, Morais, M, Rama, N, Nobre, J, Sales, I, Costa Pereira, J, Costa, S, Costa Pereira, C, Insua, C, Romero, I, Figueiredo, N, Cunha, J, Domingos, H, Vieira, P, Cunha, M, Americano, M, Amorim, E, Rachadell, J, Carvas, J, Armas, I, Fernandes, P, Pires, C, Reis, R, Martins, R, Dos Santos, M, Henriques, P, Oliveira, O, Duarte, M, Ferreira, L, Miranda, J, Vilela, N, Corte Real, J, Carlos, S, Frois Borges, M, Moniz Pereira, P, Simoes, J, Silva-vaz, P, Bettencourt, V, Gouveia, A, Perez, H, Rainho, R, Bintintan, V, Ciuce, C, Dindelegan, G, Scurtu, R, Seicean, R, Cristian, D, Burcos, T, Grama, F, Mandi, D, Richiteanu, G, Miron, A, Calu, V, Enciu, O, Nadragea, M, Parvuletu, R, Mogoanta, S, Crafciuc, A, Paitici, S, Beuran, M, Ciubotaru, C, Prodan, A, Vartic, M, Tomulescu, V, Copaescu, C, Yanishev, A, Abelevich, A, Kokobelyan, A, Lebedeva, M, Luzan, R, Pozdnyakov, A, Cherdancev, D, Mahotin, D, Nesytykh, A, Samsonyuk, V, Pravosudov, I, Ivlev, D, Karachun, A, Lebedev, K, Samsonov, D, Aiupov, R, Feoktistov, D, Garipov, M, Suleymanov, N, Tarasov, N, Rasulov, A, Dzhumabaev, H, Mamedli, Z, Bedzhanyan, A, Popov, D, Sednev, A, Klimenko, A, Semenov, A, Vasilyev, S, Khazov, A, Khanevich, M, Khrykov, G, Katorkin, S, Andreev, P, Chernov, A, Davidova, O, Zhuravlev, A, Achkasov, S, Shakhmatov, D, Shelygin, Y, Sushkov, O, Vardanyan, A, Ilkanich, A, Barbashinov, N, Darvin, V, Onishchenko, S, Voronin, Y, Krivokapic, Z, Barisic, G, Dimitrijevic, I, Markovic, V, Sekulic, A, Stanojevic, G, Brankovic, B, Nestorovic, M, Pecic, V, Petrovic, D, Kostic, I, Aleksic, A, Dabic, D, Maric, B, Perunicic, V, Radovanovic, Z, Djuric, M, Lukic, D, Radovanovic, D, Cuk, V, Juloski, J, Kenic, M, Krdzic, I, Ngu, J, Ng, Y, Teo, N, Korcek, J, Lazorisak, A, Rems, M, Ramovs Trampus, S, Tomazic, A, Grosek, J, Kosir, J, Norcic, G, Vigorita, V, Caceres, N, Casal, E, Ruano, A, Trostchansky, I, Golda, T, Galvez Saldana, A, Kreisler Moreno, E, Lopez Dominguez, J, Vila Tura, M, Labarga, F, Galvez, P, Maderuelo, V, Suero, C, Bargallo, J, Cayetano, L, Lamas, S, Silva, M, Bernal-Sprekelsen, J, Gomez, R, Jareno, S, Rios, A, Vercher, D, Garcia-Gonzalez, J, Cervera-Aldama, J, Ramos-Prada, J, Santamaria-Olabarrieta, M, Borda, N, Enrriquez-Navascues, J, Saralegui, Y, Calero-Lillo, A, Aznar-Puig, S, Lopez-Lara, M, Munoz-Collado, S, Valverde-Sintas, J, Menendez, P, Leon, C, Truan, N, Baldonedo, R, Fernandez-Martinez, D, Otero, J, Solar-Garcia, L, Turrado-Rodriguez, V, de Lacy Oliver, F, Lacy Fortuny, A, Martin Perez, B, Otero Pineiro, A, Paredes, J, Fernandez, F, Ladra, M, Paulos, A, Prieto, D, de Heredia, J, Blanco Antona, F, de Andres Asenjo, B, Ferreras Garcia, C, de Diego, A, de Laspra, E, Echazarreta Gallego, E, Elia Guedea, M, Escola, D, Martinez, S, Primo Romaguera, V, Parreno, R, Pastor, L, Rosell, E, Lozoya Trujillo, R, Alos Company, R, Ruiz Carmona, M, Solana Bueno, A, Salvans Ruiz, S, Alonso Goncalves, S, Jimenez- Toscano, M, Pascual Damieta, M, Pera Roman, M, Pellicer-Franco, E, Garcia-Marin, J, Mengual-Ballester, M, Soria-Aledo, V, Valero-Navarro, G, Vicente-Ruiz, M, Garcia-Zamora, C, Gonzalez-Gil, A, Montoya-Tabares, M, Paredes-Quiles, M, Die Trill, J, Abadia, P, Moreno, I, Pina, J, Ramos Rubio, D, Escartin, J, Blas, J, Fernando, J, Ferrer, R, Garcia Egea, J, Pros, I, Martinez, W, Rius, J, Socias, M, Sabia, D, Castellvi Valls, J, Gonzalez Santin, V, Mompart Garcia, S, Viso Pons, L, Julia, D, Codina-Cazador, A, Farres, R, Gomez, N, Planellas, P, Cuadrado, M, Camps, I, Rufas, M, Escoll, J, Ferminan, A, Muriel, P, Sierra, E, Alvarez-Laso, C, Lora, P, Padin, H, Garcia-Septiem, J, Bustamante, C, Jimenez, V, Jimenez-Miramon, J, Ramos, J, Gallardo, A, Benito, P, Colao, L, Galindo, P, Garcia, C, Forero-Torres, A, Alonso Poza, A, Dieguez Fernandez, B, Gilsanz Martin, C, Hernandez Garcia, M, Rojo Lopez, J, Gil Lopez, J, Gonzalez Zunzarren, M, Martinez Alegre, J, Zorrilla Matilla, L, Navarro-Sanchez, A, Alcala Serrano, F, Lopez-Fernandez, J, Montesdeoca Cabrera, D, Alvarez-Gallego, M, Guevara, J, Pascual Miguelanez, I, Rubio-Perez, I, Gomez Ruiz, M, Alonso Martin, J, Cagigas Fernandez, C, Castillo Diego, J, Pando, J, Maristany, C, Munoz-Duyos, A, Rada-Palomino, A, Vargas-Pierola, H, Pena Ros, E, Benavides Buleje, J, Munoz Camarena, J, Parra Banos, P, Ramirez Faraco, M, Arenal, J, Citores, M, Marcos, J, Sanchez, J, Tinoco, C, Garcia Florez, L, Arias Pacheco, R, Minguez Ruiz, G, Gutierrez Corral, N, Rodriguez Infante, A, Carrillo Lopez, M, Carrasco Prats, M, Lage Laredo, A, Martinez Manzano, A, Rodriguez Garcia, P, Segura-Sampedro, J, Alonso-Hernandez, N, Fernandez Isart, M, Gamundi Cuesta, M, Ochogavia Segui, A, Ibanez, N, Abrisqueta, J, Lujan, J, Gomez Perez, R, Corrales Valero, E, Monje Salazar, C, Sanchiz Cardenas, E, Soler Humanes, R, Jimenez-Rodriguez, R, De la Portilla, F, Diaz Pavon, J, Garcia Cabrera, A, Reyes Diaz, M, Espin, E, Marinello, F, Marti, M, Vallribera, F, Orts Mico, F, Ortin Navarro, M, Perez Climent, M, Serra Diaz, C, Millan, M, Caro, A, Escuder, J, Espina, B, Feliu, F, Climent Aira, A, Estevez Diz, A, Moreno Asencio, M, Varela Mato, A, Vazquez Bouzan, R, Diez-Alonso, M, Villeta-Plaza, R, Guadalajara, H, Alias, D, Garcia Olmo, D, Pastor, C, Valverde, I, Sanchez Romero, A, Gardea, A, Gil Santos, M, Nimmersgern, T, Serrano Paz, P, Romero-Simo, M, Blasco-Segura, T, Caravaca-Garcia, I, Costa-Navarro, D, Zarco-Pleguezuelos, A, Flor-Lorente, B, Garcia-Granero, A, Garcia-Granero, E, Arencibia, B, Alonso, J, Febles, G, Nogues, E, Roque, C, Segelman, J, Nygren, J, Nestler, G, Abraham-Nordling, M, Egenvall, M, Myrelid, P, Jung, B, Loftas, P, Lydrup, M, Azahr, N, Buchwald, P, Mangell, P, Syk, I, Nikberg, M, Carlander, J, Chabok, A, Smedh, K, Tiselius, C, Haapaniemi, S, Benckert, A, Adamina, M, Freil-Lanter, C, Gingert, C, Muller, P, Schafli, J, Regusci, L, Brenna, M, Fasolini, F, Misteli, H, Kirchhoff, P, Oertli, D, Hahnloser, D, Clerc, D, Hubner, M, Ris, F, Chevallay, M, Morel, P, Schiltz, B, Wang, J, Su, W, Huang, C, Ma, C, Tsai, H, Bugra, D, Agalar, F, Baloglu, H, Basoglu, I, Okkabaz, N, Binboga, E, Biricik, A, Celik, A, Yavuz, E, Canda, A, Agalar, C, Fuzun, M, Sokmen, S, Terzi, C, Isik, A, Karip, B, Bilgili, A, Leventoglu, S, Aytac, B, Kucukdiler, E, Yildiz, A, Yuksel, O, Sinan, H, Hancerliogullari, O, Kaymak, S, Kozak, O, Ozer, M, Sarici, I, Akca, O, Kalayci, M, Kara, Y, Agcaoglu, O, Balik, E, Bayram, O, Ozbalci, G, Ozkan, B, Karabacak, U, Sungurtekin, U, Ozgen, U, Demirbas, S, Ozturk, E, Isik, O, Yilmazlar, T, Colak, E, Karagul, S, Kinas, V, Fearnhead, N, Lord, I, Stewart, P, Zammit, M, Arnold, S, Broadhurst, J, Moran, S, Seretis, F, Shabbir, J, Jones, C, Kynaston, J, Vimalachandran, D, Blower, E, Mcfaul, C, Mcwhirter, D, Pilkington, J, Wilson, T, Chowdhary, M, Stubbs, B, Abdalkoddus, M, Lai, C, Thavanesan, N, Yao, C, Agarwal, T, Dindyal, S, Hill, R, Reade, S, Slesser, A, Paterson, H, Balfour, A, Boland, M, Geraghty, A, O'Kelly, J, Patel, P, Tezas, S, Yahia, S, Jadhav, V, Marimuthu, K, Narayanan, A, Piramanayagam, B, Bradley, N, Buchanan, F, Paul, K, Singh, J, Thomson, K, Korsgen, S, Bedford, M, Lee, K, Leong, K, Mcarthur, D, Malik, S, Mohamed, I, Cunha, P, Pilavas, A, Reddy, A, Ahmed, S, Ahmed, A, Voll, J, Velchuru, V, Lal, R, Mirshekar-Syahkal, B, Kassai, M, Aleem, M, Keogh-Bootland, S, Sarmah, P, Keegan, R, Kelkar, A, Sen, P, Oliveira-Cunha, M, Chaudhri, S, Fares, R, Thomas, W, Aslam, M, Boyle, K, Hemingway, D, Miller, A, Norwood, M, Gurjar, S, Al-Saeedi, M, Anandan, L, Sudlow, A, Zampitis, N, Malik, K, Bogdan, M, Smart, C, Iqbal, M, Bailey, S, Lawes, D, Omar, G, Tamhane, R, Evans, M, Ather, S, Lim, J, Nageswaran, H, Taylor, G, Hunt, L, Nicholls, J, Shaikh, I, Muscara, F, O'Brien, J, Photi, E, Stearns, A, Meylemans, D, Cunningham, C, Hompes, R, Tennakoon, A, Kumarasinghe, N, Rao, M, Upanishad, I, Khan, J, Ahmad, N, Shweejawee, Z, Stefan, S, Smart, N, Daniels, I, Gregoir, T, Longstaff, L, Mcdermott, F, Varcada, M, Drami, I, Gala, T, Moggia, E, Ratnatunga, K, Harries, R, Hayes, J, Williams, G, Raymond, T, Bronder, C, Davies, E, Hawkin, P, Ryska, O, Ayral, K, Beveridge, A, Bhowmik, A, Gill, M, Simpson, R, Schofield, A, Mcardle, K, Parmar, M, Williamson, M, Burton, H, Courtney, E, Grant, C, Saracino, A, Newton, K, Epstein, J, Branagan, G, Bignell, M, Symankewicz, M, Zaman, S, Mankotia, R, Siddiqui, Z, Torrance, A, Artioukh, D, Eggleston, M, Gokul, K, Selwyn, D, Warusavitarne, J, Chandrasinghe, P, Grainger, J, Leo, C, Harris, G, Levy, B, Skull, A, Thaha, M, Garg, A, Patel, H, Ramsanahie, A, Mondragon-Pritchard, M, Cuinas Leon, K, Shukla, A, Brewer, H, Fitzgerald, J, Kho, H, Torkington, J, Tate, S, Wheat, J, Smolarek, S, Platt, E, Rossi, B, Tham, J, Knight, J, Richardson, J, Tzivanakis, A, Gregori, M, Ashraf, M, Atif, M, Santos, J, Saffaf, N, Canning, L, Chandratreya, N, Bowen, M, Graham, B, Hamad, Y, Kaubrys, M, Chaudhry, Z, Bhan, C, Mukhtar, H, Oshowo, A, Wilson, J, Gouvas, N, Nicol, D, Pandey, S, Zilvetti, M, Sharma, A, Fatayer, T, Mothe, S, Rahman, M, Curtis, N, Allison, A, Dalton, R, Francis, N, Ockrim, J, Psaras, G, Dudarovaska, H, Marharint, T, Mostovoy, E, Voloshin, S, Kolesnik, O, Makhmudov, D, Altinel, Y, Iqbal, A, Cunningham, L, Go, K, Tan, S, R. Tutino, G. Salamone, L. Licari, G. Cocorullo, G. Gulotta, AGEM - Digestive immunity, Surgery, AGEM - Re-generation and cancer of the digestive system, CCA - Cancer Treatment and Quality of Life, RS: NUTRIM - R2 - Liver and digestive health, and 2017 and 2015 European Society of Coloproctology (ESCP) collaborating, Group
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Laparoscopic surgery ,Male ,Colorectal cancer ,medicine.medical_treatment ,surgery ,0302 clinical medicine ,Postoperative Complications ,Medicine ,Major complication ,Prospective Studies ,Laparoscopy ,SHORT-TERM OUTCOMES ,Colectomy ,Medical Audit ,medicine.diagnostic_test ,Prospective audit ,Gastroenterology ,Middle Aged ,laparoscopic surgery ,Conversion to Open Surgery ,Colon cancer ,Treatment Outcome ,Elective Surgical Procedures ,030220 oncology & carcinogenesis ,030211 gastroenterology & hepatology ,Female ,Colonic surgery ,Adult ,medicine.medical_specialty ,CLINICAL-TRIAL ,NO ,OPEN COLECTOMY ,03 medical and health sciences ,COLORECTAL SURGERY ,Case mix index ,gastrointestinal surgery ,Humans ,rectal cancer ,RECTAL-CANCER ,Aged ,ta3126 ,business.industry ,Elective resection ,medicine.disease ,RANDOMIZED-TRIAL ,OPEN RESECTION ,Surgery ,business - Abstract
BACKGROUND: Laparoscopy has now been implemented as a standard of care for elective colonic resection around the world. During the adoption period, studies showed that conversion may be detrimental to patients, with poorer outcomes than both laparoscopic completed or planned open surgery. The primary aim of this study was to determine whether laparoscopic conversion was associated with a higher major complication rate than planned open surgery in contemporary, international practice.METHODS: Combined analysis of the European Society of Coloproctology 2017 and 2015 audits. Patients were included if they underwent elective resection of a colonic segment from the caecum to the rectosigmoid junction with primary anastomosis. The primary outcome measure was the 30-day major complication rate, defined as Clavien-Dindo grade III-V.RESULTS: Of 3980 patients, 64% (2561/3980) underwent laparoscopic surgery and a laparoscopic conversion rate of 14% (359/2561). The major complication rate was highest after open surgery (laparoscopic 7.4%, converted 9.7%, open 11.6%, P < 0.001). After case mix adjustment in a multilevel model, only planned open (and not laparoscopic converted) surgery was associated with increased major complications in comparison to laparoscopic surgery (OR 1.64, 1.27-2.11, P < 0.001).CONCLUSIONS: Appropriate laparoscopic conversion should not be considered a treatment failure in modern practice. Conversion does not appear to place patients at increased risk of complications vs planned open surgery, supporting broadening of selection criteria for attempted laparoscopy in elective colonic resection.
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- 2018
18. José Antonio CARRO CELADA, Jesucristo en la literatura española e hispanoamericana del siglo XX, La Editorial Católica (BAC), Madrid 1997, 142 pp.
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De Navascués, J., primary
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- 2018
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19. The Behaviour of Two Cell Populations in the Pericycle of Allium cepa, Pisum sativum, and Daucus carota During Early Lateral Root Development
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LLORET, P. G., CASERO, P. J., PULGARÍN, A., and NAVASCUÉS, J.
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- 1989
20. The Relationship Between Lateral-Root Distribution and Endodermis and Pericycle Cell Length in Allium cepa L. Adventitious Roots
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LLORET, P. G., VIDAL, M. R., CASERO, P. J., and NAVASCUES, J.
- Published
- 1985
21. Cell Wall Thickening of the Xylem Tracheary Elements in Different Zones of the Adventitious Root of Allium cepa L.
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LLORET, P. G., CASERO, P. J., VIDAL, M. R., ABADIA-FENOLL, F., and NAVASCUES, J.
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- 1986
22. AECP Report, 2017
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Enríquez-Navascués, J. M., primary, Espín-Basany, E., additional, and Jiménez-Escobar, F., additional
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- 2017
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23. Necesidad del empleo de muestras significativas en los recuentos celulares
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Navascués, J., primary, Almendros, A., additional, and Abadía Fenoll, F., additional
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- 2016
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24. Extra-axonal environment and fibre directionality in the early development of the chick embryo optic chiasm: A light and scanning electron microscopic study
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Navascués, J., Rodríguez-Gallardo, L., García-Martínez, V., Alvarez, I. S., and Martín-Partido, G.
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- 1987
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25. Changes in cell length and mitotic index in vascular pattern-related pericycle cell types along the apical meristem and elongation zone of the onion root
- Author
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Casero, P. J., García-Sánchez, C., Lloret, P. G., and Navascués, J.
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- 1989
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26. Glioblast migration in the optic stalk of the chick embryo
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Navascués, J., Martín-Partido, G., Alvarez, I. S., Rodríguez-Gallardo, L., and García-Martínez, V.
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- 1987
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27. Proliferation of glial precursors during the early development of the chick optic nerve
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Navascués, J., Rodriguez-Gallardo, L., Martín-Partido, G., and Alvarez, I. S.
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- 1985
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28. Characterization of variants in the glucosylceramide synthase gene and their association with type 1 Gaucher disease severity
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Alfonso P, Navascués J, Navarro S, Medina P, Bolado-Carrancio A, Andreu V, Irún P, Rodríguez-Rey JC, Pocoví M, España F, and Giraldo P
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Gaucher disease, UGCG, glucosylceramide synthase, phenotype variability - Abstract
The extreme phenotypic variability of patients with Gaucher disease (GD) is not completely explained by glucocerebrosidase gene mutations. It has been proposed that genetic modifiers might influence GD phenotype. We examined seven polymorphisms of the glucosylceramide synthase gene (UGCG) and their correlation with severity of GD. Five UGCG variants were significantly associated with disease severity, according to the DS3 scoring system: c.-295C>T, c.-232_-241ins10, c.98+50A>G, c.98+68A>T, and c.861A>G. Heterozygous [N370S]+[L444P] patients with c.[-232_-241ins10;98+50G] haplotype had a significantly lower DS3 score in relation to patients carrying only one of these polymorphisms. Electrophoretic mobility shift assay analysis showed an increased nuclear protein binding ability for the G allele at the cDNA position c.98+50, as well as an altered pattern for the c.-232_-241ins10 allele. The promoter activity of the haplotypes decreased significantly with respect to wild type activity in HepG2 and COS-7 cells (-14% and -16% for the c.[-232_-241ins10;98+50A] haplotype, -44% and -25% for c.[-222nonins;98+50G] haplotypes, and -64% and -75% for c.[-232_-241ins10;98+50G] haplotype, respectively). These data indicate that the c.-232_-241ins10 and c.98+50A>G variants are modifying factors of GD severity, which can partly explain the variability in severity of the disease.
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- 2013
29. Treatment of anastomotic leaks with metallic stent after esophagectomies
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Eizaguirre, E., primary, Larburu, S., additional, Asensio, J. I., additional, Rodriguez, A., additional, Elorza, J. L., additional, Loyola, F., additional, Urdapilleta, G., additional, and Navascués, J. M. E., additional
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- 2015
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30. Restos de una necrópolis orientalizante en la desembocadura del río Aljucén (Mérida, Badajoz)
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Domínguez de la Concha, Coronada and Enríquez Navascués, J. J.
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Humanidades ,lcsh:C ,lcsh:Archaeology ,lcsh:CC1-960 ,UNESCO::HISTORIA::Ciencias auxiliares de la historia::Arqueología ,Historia ,lcsh:Auxiliary sciences of history ,HISTORIA::Ciencias auxiliares de la historia::Arqueología [UNESCO] - Published
- 1991
31. Intrapelvic placement of a breast implant to allow deferred ileoanal pouch anastomosis after emergency proctocolectomy
- Author
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Enríquez-Navascués, J. M., primary, Alkorta, M., additional, Borda, N., additional, and Placer, C., additional
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- 2013
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32. Antioxidants and reactive oxygen/nitrogen species in legume root nodules
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Becana⁎, M., primary, Matamoros, M., additional, Saiz, A., additional, Navascués, J., additional, Pérez-Rontomé, C., additional, Wienkoop, S., additional, Sainz, M., additional, and Ramos et al, J., additional
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- 2012
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33. Nuclear organization and dynamics of transcription sites in rat sensory ganglia neurons detected by incorporation of 5′-fluorouridine into nascent RNA
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Casafont, I., primary, Navascués, J., additional, Pena, E., additional, Lafarga, M., additional, and Berciano, M.T., additional
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- 2006
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34. Paediatric Trauma in Spain: A Report from the HUGM Trauma Registry
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Navascués, J. A., primary, Matute, J., additional, Soleto, J., additional, García Casillas, M. A., additional, Hernández, E., additional, Sánchez-París, O., additional, Molina, E., additional, De Tomás, E., additional, Cerdá, J., additional, Romero, R., additional, De Agustín, J. C., additional, Aguilar, F., additional, and Vázquez, J., additional
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- 2005
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- View/download PDF
35. Premature Physeal Closure After Tibial Diaphyseal Fractures in Adolescents
- Author
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Navascués, J. A., primary, González-López, J. L., additional, López-Valverde, S., additional, Soleto, J., additional, Rodriguez-Durantez, J. A., additional, and García-Trevijano, J. L., additional
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- 2000
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- View/download PDF
36. Differential staining of dead and dying embryonic cells with a simple new technique.
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Martín-Partido, G., Alvarez, I. S., Rodríguez-Gallardo, L., and Navascués, J.
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- 1986
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37. Association of mechanical bowel preparation with oral antibiotics and anastomotic leak following left sided colorectal resection: an international, multi-centre, prospective audit
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The 2017 European Society of Coloproctology (ESCP) collaborating group, Blanco-Colino, Kelly, Singh, Bhangu, Pinkney, Poskus, El-Hussuna, Battersby, N. J., Buchs, N. C., Buskens, Chaudri, Frasson, Gallo, Minaya-Bravo, A. M., Morton, Negoi, Nepogodiev, Pata, Sánchez-Guillén, Zmora, Perry, Magill, Altomare, Bemelman, Brown, Denost, Knowles, Laurberg, Lefevre, J. H., Möeslein, Vaizey, Bilali, Salomon, Cillo, Estefania, Patron, Uriburu, Ruiz, Farina, Carballo, Guckenheimer, Proud, Brouwer, Bui, Nguyen, Smart, Warwick, Theodore, J. E., Herbst, Birsan, Dauser, Ghaffari, Hartig, Stift, Argeny, Unger, Strouhal, Heuberger, Varabei, Lahodzich, Makhmudov, Selniahina, Feryn, Leupe, Maes, Reynvoet, Van, Langenhove, Nachtergaele, Monami, Francart, Jehaes, Markiewicz, Weerts, Van, Belle, Bomans, Cavenaile, Nijs, Vertruyen, Pletinckx, Claeys, Defoort, Muysoms, Van, Cleven, Lange, Vindevoghel, Wolthuis, Todorovic, Dabic, Kenjic, Lovric, Vidovic, Delibegovic, Mehmedovic, Christiano, Lombardi, Marchiori, J, R., Tercioti, Dardanov, Petkov, Simonova, Yonkov, Zhivkov, Maslyankov, Pavlov, Sokolov, Todorov, Stoyanov, Batashki, Iarumov, Lozev, Moshev, Slavchev, Atanasov, Belev, Krstev, Penkov, Šantak, Ćosić, Previšić, Vukušić, Zukanović, Zelić, Kršul, Lekić, Vitlov, Mendrila, Orhalmi, Dusek, Maly, Paral, Sotona, Skrovina, Bencurik, Machackova, Kala, Farkašová, Grolich, Procházka, Hoch, Kocian, Martinek, Antos, Pruchova, Ceccotti, Madsbøll, Straarup, Uth, Ovesen, Christensen, Bondeven, Edling, Elfeki, Alexandrovich, Gameza, Michelsen, Bach, Zheltiakova, Krarup, P. M., Krogh, Rolff, H. -C., Lykke, Juvik, A. F., Lóven, H. H. K., Marckmann, Osterkamp, J. T. F., Madsen, A. H., Worsøe, Ugianskis, Kjær, M. D., Youn Cho Lee, Khalid, Kristensen, M. H., Sorogy, El, Elgeidie, Elhemaly, Nakeeb, El, Elrefai, Shalaby, Emile, Omar, Sakr, Thabet, Awny, Metwally, Refky, Shams, Zuhdy, Lepistö, Keränen, Kivelä, Lehtonen, Siironen, Rautio, Ahonen-Siirtola, Klintrup, Paarnio, Takala, Hyöty, Haukijärvi, Kotaluoto, S. -M., Lehto, Tomminen, Huhtinen, Carpelan, Karvonen, Rantala, Varpe, Cotte, Francois, Glehen, Passot, D'Alessandro, Chouillard, Etienne, J. C., Ghilles, Vinson-Bonnet, Germain, Ayav, Bresler, Chevalier, Didailler, Rullier, Tiret, Chafai, Parc, Sielezneff, Mege, Lakkis, Barussaud, Krones, Bock, Webler, Baral, Lang, Münch, Pullig, Schön, Hinz, Becker, Möller, Richter, Schafmayer, Hardt, Kienle, Crescenti, Ahmad, Soleiman, Papaconstantinou, Gklavas, Nastos, Theodosopoulos, Vezakis, Stamou, Saridaki, Xynos, Paraskakis, Zervakis, Skroubis, Amanatidis, Germanos, Maroulis, Papadopoulos, Dimitriou, Alexandrou, Felekouras, Griniatsos, Karavokyros, Chouliaras, Ioannidis, Katsounis, Kefalou, Katsoulis, I. E., Balalis, Korkolis, D. P., Manatakis, Tzovaras, Baloyiannis, Mamaloudis, Lázár, Ábraham, Paszt, Simonka, Tóth, Zaránd, Baranyai, Ferreira, Harsányi, Ónody, Banky, Burány, Lakatos, Marton, Solymosi, Besznyák, Bursics, Papp, Saftics, Svastics, Valsdottir, Atladottir, Jonsson, Moller, Sigurdsson, Gupta, S. K., Kaul, Mohan, Sharma, Wani, Chowdri, Khan, Mehraj, Parray, F. Q., Coveney, Burke, Deasy, El-Masry, Mcnamara, Cahill, Faul, Mulsow, Shields, Bass, Martin, S. T., O'Connell, Ryan, Connelly, Bukhari, Cooke, Gold, Deutch, Haim, Lavy, Moscovici, Shussman, Gefen, Marom, Pikarsky, Weiss, Avital, Hermann, Raguan, Slavin, White, Wasserberg, Arieli, Gurevich, Freund, M. R., Dorot, Edden, Halfteck, Reissman, Pery, Tulchinsky, Weizman, Agresta, Curinga, Finotti, Savino, Verza, L. A., Asteria, C. R., Boccia, Pascariello, Tamini, Bugatti, Gianotti, Totis, Vincenti, Andriola, Giannini, Travaglio, Balestri, Buccianti, Roffi, Rossi, Urbani, Mellano, Cinquegrana, Lauretta, Belluco, Mistrangelo, Allaix, M. E., Arolfo, Morino, Testa, Delrio, Pace, Rega, Scala, Clerico, Cornaglia, Realis, Luc, Trompetto, Ugolini, Antonacci, Fabbri, Montroni, Zattoni, D'Urbano, Cornelli, Viti, Inama, Bacchion, Casaril, Impellizzeri, Moretto, Spinelli, Carvello, David, Candido, Di, Sacchi, Frontali, Ceriani, Molteni, Rosati, Aleotti, Elmore, Lemma, Vignali, Scabini, Casoni, Pattacini, Luzzi, Romairone, Marino, Lorusso, Pezzolla, Colombo, Baldi, Foschi, Sampietro, Sorrentino, Saverio, Di, Birindelli, Segalini, Spacca, Romano, G. M., Belli, Bianco, Franciscis, De, Falato, Muratore, Marsanic, Grimaldi, Castaldo, Ciolli, M. G., Picarella, Porfidia, Tugnoli, Bondurri, Cavallo, Maffioli, Pertusati, Pulighe, Balestra, Nisco, De, Podda, Opocher, Longhi, Mariani, N. M., Maroni, Pisani, Ceretti, Galleano, Aonzo, Curletti, Reggiani, Marconi, Del, Prete, Oldani, Pappalardo, Zaccone, L, Scatizzi, Baraghini, Cantafio, Feroci, Giani, Tutino, Cocorullo, Gulotta, Licari, Salamone, Sileri, Saraceno, Torre, La, Chirletti, Coletta, Toma, De, Mingoli, Papandrea, Luca, De, Sacco, Sammarco, Vescio, Tonini, Bianchini, Cervellera, Vaccari, Cracco, Barugola, Bertocchi, Rossini, Ruffo, Sartori, Clemente, Scaffidi, Lorenzon, Balducci, Bocchetti, Ferri, Mercantini, Bauce, Benevento, Bottini, Crapa, P. R., Rubbini, Michele, Anania, Gabriele, Carcoforo, Paolo, Cavallesco, Narciso Giorgio, Feo, Carlo, Yamamoto, Sivins, Ancans, Gerkis, Lunis, Pcolkins, Venskutonis, Bradulskis, Dainius, Subocius, Vencius, Zeromskas, Eismontas, Nutautiene, Simcikas, Tamosiunas, Svagzdys, Latkauskas, Lizdenis, Saladzinskas, Tamelis, Dulskas, Kuliavas, Samalavicius, N. E., Jotautas, Mikalauskas, Strupas, Zakaria, A. D., Lah, N. N., Wong, Zain, W. Z., Mazlan, Mohd, Azman, Z. 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Y., Raza, Urooj, Wang, Jajtner, Gasinski, Kabiesz, Walega, Romaniszyn, Zawadzki, Czarnecki, Obuszko, Rzaca, Sitarska, Silva, Duarte, Gonçalves, Morais, Rama, Nobre, Sales, Costa, Pereira, Costa, Insua, Romero, Figueiredo, Cunha, Domingos, Vieira, Americano, Amorim, Rachadell, Carvas, J. M., Armas, Fernandes, Pires, Reis, Martins, Dos, Santos, Henriques, Oliveira, Miranda, Vilela, Corte, Real, Carlos, Frois, Borges, Moniz, Pereira, Simões, Silva-vaz, Bettencourt, Gouveia, Perez, Rainho, Bintintan, Ciuce, Dindelegan, Scurtu, Seicean, Cristian, Burcos, Grama, Mandi, D. M., Richiteanu, Miron, Calu, Enciu, Nadragea, Parvuletu, Mogoanta, S. S., Crafciuc, Paitici, Beuran, Ciubotaru, Prodan, Vartic, Tomulescu, Copaescu, Yanishev, Abelevich, Kokobelyan, Lebedeva, Luzan, Pozdnyakov, Cherdancev, Mahotin, Nesytykh, Samsonyuk, Pravosudov, Ivlev, Karachun, Lebedev, Samsonov, Aiupov, Feoktistov, Garipov, Suleymanov, Tarasov, Rasulov, Dzhumabaev, Mamedli, Bedzhanyan, Popov, Sednev, Klimenko, Semenov, Vasilyev, Khazov, Khanevich, Khrykov, Katorkin, Andreev, Chernov, Davidova, Zhuravlev, Achkasov, Shakhmatov, Shelygin, Sushkov, Vardanyan, Ilkanich, Barbashinov, Darvin, Onishchenko, Voronin, Krivokapić, Barišić, Dimitrijević, Marković, Sekulić, Stanojevic, Brankovic, Nestorovic, Pecic, Petrovic, Kostic, Aleksic, Maric, Perunicic, Radovanovic, Djuric, Lukic, Cuk, Juloski, Kenic, Krdzic, Ngu, Y. Y., Teo, Korcek, Lazorisak, Rems, Ramovš, Trampuš, Tomazic, Grosek, Kosir, Norcic, Vigorita, Caceres, Casal, Ruano, Trostchansky, Golda, Galvez, Saldaña, Kreisler, Moreno, Lopez, Dominguez, Vila, Tura, Labarga, Galvez, Maderuelo, Suero, Bargallo, Cayetano, Lamas, M. C., Bernal-Sprekelsen, Gómez, Jareño, Ríos, Vercher, García-González, J. -M., Cervera-Aldama, Ramos-Prada, Santamaría-Olabarrieta, Borda, Enrriquez-Navascués, Saralegui, Calero-Lillo, Aznar-Puig, López-Lara, Muñoz-Collado, Valverde-Sintas, Menendez, Leon, Truan, Baldonedo, Fernández-Martínez, Otero, Solar-García, Turrado-Rodriguez, de Lacy Oliver, Lacy, Fortuny, Martin, Perez, Otero, Piñeiro, Paredes, Fernandez, Ladra, M. J., Paulos, Prieto, Beltrán de Heredia, J. P., Blanco, Antona, de Andrés Asenjo, Ferreras, García, Romero de Diego, Cordoba Diaz de Laspra, Echazarreta, Gallego, Elia, Guedea, Escola, Martinez, Primo, Romaguera, Parreño, Pastor, Rosell, Lozoya, Trujillo, Alós, Company, Ruiz, Carmona, Solana, Bueno, Salvans, Ruiz, Alonso, Gonçalves, Jiménez-Toscano, Pascual, Damieta, Pera, Roman, Pellicer-Franco, E. M., Garcia-Marin, J. A., Mengual-Ballester, Soria-Aledo, Valero-Navarro, Vicente-Ruiz, Garcia-Zamora, Gonzalez-Gil, Montoya-Tabares, Paredes-Quiles, Die, Trill, Abadia, Moreno, Pina, J. D., Ramos, Rubio, Escartin, Blas, J. L., Fernando, Ferrer, Garcia, Egea, Pros, Rius, Socías, Sabia, Castellvi, Valls, Gonzalez, Santin, Mompart, Garcia, Viso, Pons, Julià, Codina-Cazador, Farrés, Planellas, Cuadrado, Camps, Rufas, Escoll, Fermiñán, Muriel, Sierra, Alvarez-Laso, Lora, Padin, Garcia-Septiem, Bustamante, Jimenez, Jimenez-Miramon, Ramos, Gallardo, A. 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T., Varela, Mato, Vázquez, Bouzán, Diez-Alonso, Villeta-Plaza, Guadalajara, Alías, García, Olmo, Valverde, Sanchez, Romero, Gardea, Gil, Santos, Nimmersgern, Serrano, Paz, Romero-Simó, Blasco-Segura, Caravaca-García, Costa-Navarro, Zarco-Pleguezuelos, Flor-Lorente, García-Granero, Arencibia, Alonso, Febles, Nogués, Roque, Segelman, Nygren, Nestler, Abraham-Nordling, Egenvall, Myrelid, Jung, Loftås, Lydrup, M. -L., Azahr, Buchwald, Mangell, Syk, Nikberg, Carlander, Chabok, Smedh, Tiselius, Haapaniemi, Benckert, Adamina, Freil-Lanter, Gingert, Müller, Schäfli, Regusci, Brenna, Fasolini, Misteli, Kirchhoff, Oertli, Hahnloser, Clerc, Hübner, Ris, Chevallay, Morel, Schiltz, J. Y., W. -C., Huang, C. -W., C. -J., Tsai, H. -L., Bugra, Agalar, Baloglu, Basoglu, Okkabaz, Binboga, Biricik, Celik, Yavuz, Canda, A. E., Fuzun, Sokmen, Terzi, Isik, Karip, Bilgili, A. C., Leventoğlu, Aytac, Küçükdiler, Yıldız, Yuksel, Sinan, Hancerliogullari, Kaymak, Kozak, Ozer, Sarici, I. S., Akca, Kalayci, M. 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I., Boyle, Hemingway, Miller, Norwood, Gurjar, Al-Saeedi, Anandan, Sudlow, Zampitis, Bogdan, Iqbal, Bailey, Lawes, Tamhane, Evans, Ather, Lim, Nageswaran, Taylor, Hunt, Nicholls, Shaikh, Muscara, O'Brien, Photi, Stearns, Meylemans, Cunningham, Hompes, Tennakoon, Kumarasinghe, Rao, Upanishad, Shweejawee, Stefan, Daniels, Gregoir, Longstaff, Mcdermott, Varcada, Drami, Gala, Moggia, Ratnatunga, Harries, Hayes, Williams, Raymond, Bronder, Davies, Hawkin, Ryska, Ayral, Beveridge, Bhowmik, Gill, Simpson, Schofield, Mcardle, Parmar, Williamson, Burton, Courtney, Grant, Saracino, Newton, Epstein, Branagan, Bignell, Symankewicz, Zaman, Mankotia, Siddiqui, Torrance, Artioukh, Eggleston, Gokul, Selwyn, Warusavitarne, Chandrasinghe, Grainger, Leo, C. A., Harris, Levy, Skull, Thaha, Garg, Ramsanahie, Mondragon-Pritchard, Cuinas, Leon, Shukla, Brewer, Fitzgerald, Kho, Torkington, Tate, Wheat, Smolarek, Platt, Tham, Knight, Richardson, Tzivanakis, Gregori, Ashraf, Atif, Santos, Saffaf, Canning, Chandratreya, Bowen, Graham, Hamad, Kaubrys, Chaudhry, Z. U., Bhan, Mukhtar, Oshowo, Gouvas, Nicol, Pandey, Zilvetti, Fatayer, Mothe, Rahman, Curtis, Allison, Dalton, Francis, Ockrim, Psaras, Dudarovaska, Marharint, Mostovoy, Voloshin, Kolesnik, Altinel, Tan, Surgery, European Society of Coloproctology (ESCP) Cohort Study, Tonini V, Cervellera M, Vaccari S., Tutino R., Salamone G., Licari L., G. Cocorullo, G. 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M., Roque, C., Segelman, J., Nygren, J., Nestler, G., Abraham-Nordling, M., Egenvall, M., Myrelid, P., Jung, B., Loftås, P., Lydrup, M. -L., Azahr, N., Buchwald, P., Mangell, P., Syk, I., Nikberg, M., Carlander, J., Chabok, A., Smedh, K., Tiselius, C., Haapaniemi, S., Benckert, A., Adamina, M., Freil-Lanter, C., Gingert, C., Müller, P., Schäfli, J., Regusci, L., Brenna, M., Fasolini, F., Misteli, H., Kirchhoff, P., Oertli, D., Hahnloser, D., Clerc, D., Hübner, M., Ris, F., Buchs, N. C., Chevallay, M., Morel, P., Schiltz, B., Wang, J. Y., Su, W. -C., Huang, C. -W., Ma, C. -J., Tsai, H. -L., Bugra, D., Agalar, F., Baloglu, H., Basoglu, I., Okkabaz, N., Binboga, E., Biricik, A., Celik, A., Yavuz, E., Canda, A. E., Agalar, C., Fuzun, M., Sokmen, S., Terzi, C., Isik, A., Karip, B., Bilgili, A. C., Leventoğlu, S., Aytac, B., Küçükdiler, E., Yıldız, A., Yuksel, O., Sinan, H., Hancerliogullari, O., Kaymak, S., Kozak, O., Ozer, M. T., Sarici, I. S., Akca, O., Kalayci, M. U., Kara, Y., Agcaoglu, O., Balik, E., Bayram, O., Özbalcı, G. S., Özkan, B. B., Karabacak, U., Sungurtekin, U., Ozgen, U., Demirbas, S., Öztürk, E., Isik, O., Yilmazlar, T., Colak, E., Karagul, S., Kinas, V., Fearnhead, N., Lord, I., Stewart, P., Zammit, M., Arnold, S., Battersby, N. J., Broadhurst, J., Moran, S., Seretis, F., Shabbir, J., Jones, C., Kynaston, J., Vimalachandran, D., Blower, E., Mcfaul, C., Mcwhirter, D., Pilkington, J., Wilson, T., Chowdhary, M., Stubbs, B., Abdalkoddus, M., Lai, C., Thavanesan, N., Yao, C., Agarwal, T., Dindyal, S., Hill, R. M. C., Reade, S., Slesser, A., Paterson, H., Balfour, A., Boland, M., Geraghty, A., O'Kelly, J., Patel, P., Tezas, S., Yahia, S., Jadhav, V., Marimuthu, K., Narayanan, A., Piramanayagam, B., Bradley, N., Buchanan, F., Paul, K., Singh, J., Thomson, K., Korsgen, S., Bedford, M., Lee, K., Leong, K., Mcarthur, D., Bhangu, A., Malik, S., Mohamed, I., Cunha, P., Pilavas, A., Reddy, A., Ahmed, S., Ahmed, A., Voll, J., Velchuru, V., Lal, R., Mirshekar-Syahkal, B., Kassai, M., Aleem, M., Keogh-Bootland, S., Sarmah, P., Brown, S., Keegan, R., Kelkar, A., Sen, P., Oliveira-Cunha, M., Chaudhri, S., Fares, R., Singh, B., Thomas, W. M., Aslam, M. I., Boyle, K., Hemingway, D., Miller, A., Norwood, M., Gurjar, S., Al-Saeedi, M., Anandan, L., Sudlow, A., Zampitis, N., Malik, K., Bogdan, M., Smart, C., Iqbal, M. R., Bailey, S., Lawes, D., Omar, G., Tamhane, R., Evans, M., Ather, S., Lim, J., Nageswaran, H., Taylor, G., Hunt, L., Nicholls, J., Shaikh, I., Muscara, F., O'Brien, J., Photi, E., Stearns, A., Meylemans, D., Cunningham, C., Hompes, R., Tennakoon, A., Kumarasinghe, N., Rao, M., Upanishad, I., Khan, J., Ahmad, N., Shweejawee, Z., Stefan, S., Smart, N., Daniels, I., Gregoir, T., Longstaff, L., Mcdermott, F., Varcada, M., Drami, I., Gala, T., Moggia, E., Ratnatunga, K., Harries, R., Hayes, J., Williams, G., Raymond, T., Bronder, C., Davies, E., Hawkin, P., Ryska, O., Ayral, K., Beveridge, A., Bhowmik, A., Gill, M., Simpson, R., Schofield, A., Mcardle, K., Parmar, M., Williamson, M., Burton, H., Courtney, E., Grant, C., Saracino, A., Newton, K., Epstein, J., Branagan, G., Bignell, M., Symankewicz, M., Zaman, S., Mankotia, R., Siddiqui, Z., Torrance, A., Artioukh, D., Eggleston, M., Gokul, K., Selwyn, D., Warusavitarne, J., Chandrasinghe, P., Grainger, J., Leo, C. A., Vaizey, C. J., Harris, G., Levy, B., Skull, A., Thaha, M., Garg, A., Patel, H., Ramsanahie, A., Mondragon-Pritchard, M., Cuinas Leon, K., Shukla, A., Brewer, H., Fitzgerald, J., Kho, H., Torkington, J., Tate, S., Wheat, J., Smolarek, S., Platt, E., Rossi, B., Tham, J. C., Knight, J., Richardson, J., Tzivanakis, A., Gregori, M., Ashraf, M. A., Atif, M., Santos, J., Saffaf, N., Canning, L., Chandratreya, N., Bowen, M., Graham, B., Hamad, Y., Kaubrys, M., Chaudhry, Z. U., Bhan, C., Mukhtar, H., Oshowo, A., Wilson, J., Gouvas, N., Nicol, D., Pandey, S., Zilvetti, M., Sharma, A., Fatayer, T., Mothe, S., Rahman, M., Curtis, N., Allison, A., Dalton, R., Francis, N., Ockrim, J., Psaras, G., Dudarovaska, H., Marharint, T., Mostovoy, E., Voloshin, S., Kolesnik, O., Makhmudov, D., Altinel, Y., Iqbal, A., Cunningham, L., Go, K., and Tan, S.
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Laparoscopic surgery ,Male ,Leak ,medicine.medical_specialty ,Cathartics/administration & dosage ,medicine.drug_class ,medicine.medical_treatment ,Antibiotics ,anastomotic leak ,bowel preparation ,colonic cancer ,Colorectal surgery ,laparoscopic surgery ,Gastroenterology ,030230 surgery ,Anastomosis ,Preoperative Care/methods ,NO ,03 medical and health sciences ,0302 clinical medicine ,Anti-Bacterial Agents/administration & dosage ,Preoperative Care ,Medicine ,Humans ,risk factors ,Prospective Studies ,Multi centre ,Prospective cohort study ,Colectomy ,Aged ,ta3126 ,Medical Audit ,business.industry ,Cathartics ,digestive, oral, and skin physiology ,Middle Aged ,digestive system diseases ,Surgery ,Anti-Bacterial Agents ,030220 oncology & carcinogenesis ,Bowel preparation ,Drug Therapy, Combination ,Female ,Colectomy/adverse effects ,business ,Anastomotic Leak/etiology - Abstract
INTRODUCTION: The optimal bowel preparation strategy to minimise the risk of anastomotic leak is yet to be determined. This study aimed to determine whether oral antibiotics combined with mechanical bowel preparation (MBP+Abx) was associated with a reduced risk of anastomotic leak when compared to mechanical bowel preparation alone (MBP) or no bowel preparation (NBP).METHODS: A pre-planned analysis of the European Society of Coloproctology (ESCP) 2017 Left Sided Colorectal Resection audit was performed. Patients undergoing elective left sided colonic or rectal resection with primary anastomosis between 1 January 2017 and 15 March 2017 by any operative approach were included. The primary outcome measure was anastomotic leak.RESULTS: Of 3676 patients across 343 centres in 47 countries, 618 (16.8%) received MBP+ABx, 1945 MBP (52.9%) and 1099 patients NBP (29.9%). Patients undergoing MBP+ABx had the lowest overall rate of anastomotic leak (6.1%, 9.2%, 8.7% respectively) in unadjusted analysis. After case-mix adjustment using a mixed-effects multivariable regression model, MBP+Abx was associated with a lower risk of anastomotic leak (OR 0.52, 0.30-0.92, P = 0.02) but MBP was not (OR 0.92, 0.63-1.36, P = 0.69) compared to NBP.CONCLUSION: This non-randomised study adds 'real-world', contemporaneous, and prospective evidence of the beneficial effects of combined mechanical bowel preparation and oral antibiotics in the prevention of anastomotic leak following left sided colorectal resection across diverse settings. We have also demonstrated limited uptake of this strategy in current international colorectal practice.
- Published
- 2018
38. Music Listening in Stem Cell Transplantation and Acute Myeloid Leukemia: A Randomized Clinical Trial.
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Lázaro-García A, Láinez-González D, González-Rodríguez M, Cano Alsua S, Suárez M EU, Solán-Blanco L, Cornago-Navascués J, López-Lorenzo JL, Llamas-Sillero P, and Alonso-Domínguez JM
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- Humans, Male, Female, Middle Aged, Adult, Treatment Outcome, Aged, Leukemia, Myeloid, Acute therapy, Quality of Life, Anxiety therapy, Music Therapy methods, Depression therapy, Stem Cell Transplantation
- Abstract
Context: Music listening (ML) has been shown to have a beneficial effect on patients with cancer. However, novel intervention approaches are needed., Objectives: We aimed to determine whether ML based on the iso-principle, conducted using a mobile application (GloMus), improves symptom burden, quality of life (QoL), anxiety, and depression in patients undergoing stem cell transplantation (SCT) and intensive induction chemotherapy for acute myeloid leukemia (AML)., Methods: In this randomized controlled clinical trial, we assigned 71 patients to the ML or standard care (SC) groups, stratified by the reason for admission (AML, allogeneic-SCT, or inpatient/outpatient autologous-SCT). Upon admission, participants in the ML groups were invited to undergo daily ML sessions designed to change negative moods into positive ones (iso-principle). The intervention consisted of listening to pre-recorded classical music ordered by beats per minute and tonality. Symptom burden (Edmonton Symptom Assessment System-Revised) was assessed in the ML groups before and after each session. Anxiety, depression (Hospital Anxiety and Depression Scale), and QoL (Functional Assessment of Cancer Therapy-Bone Marrow Transplantation/Leukemia) were measured weekly in the ML and SC groups., Results: Symptom burden in both allogeneic- and inpatient autologous-SCT ML groups reduced after the intervention. In all experimental groups, clinically important improvements were observed after ML sessions. No differences were found between the groups (ML vs. SC) at different weeks of admission regarding anxiety, depression, and QoL., Conclusions: ML based on our innovative iso-principle strategy, conducted using GloMus, reduced the symptom burden in patients undergoing allogeneic- and inpatient autologous-SCT (ClinicalTrials.gov number, NCT05696457)., (Copyright © 2024 American Academy of Hospice and Palliative Medicine. Published by Elsevier Inc. All rights reserved.)
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- 2024
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39. Upregulation of the secretory pathway Ca 2+ /Mn 2+ -ATPase isoform 1 in LPS-stimulated microglia and its involvement in Mn 2+ -induced Golgi fragmentation.
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Bhojwani-Cabrera AM, Bautista-García A, Neubrand VE, Membrive-Jiménez FA, Bramini M, Martin-Oliva D, Cuadros MA, Marín-Teva JL, Navascués J, Vangheluwe P, and Sepúlveda MR
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- Lipopolysaccharides toxicity, Microglia metabolism, Calcium-Transporting ATPases genetics, Calcium-Transporting ATPases metabolism, Secretory Pathway, Up-Regulation, Golgi Apparatus metabolism, Golgi Apparatus ultrastructure, Membrane Transport Proteins metabolism, Protein Isoforms metabolism, Calcium metabolism, Adenosine Triphosphatases metabolism, Curcumin metabolism
- Abstract
Microglia play an important protective role in the healthy nervous tissue, being able to react to a variety of stimuli that induce different intracellular cascades for specific tasks. Ca
2+ signaling can modulate these pathways, and we recently reported that microglial functions depend on the endoplasmic reticulum as a Ca2+ store, which involves the Ca2+ transporter SERCA2b. Here, we investigated whether microglial functions may also rely on the Golgi, another intracellular Ca2+ store that depends on the secretory pathway Ca2+ /Mn2+ -transport ATPase isoform 1 (SPCA1). We found upregulation of SPCA1 upon lipopolysaccharide stimulation of microglia BV2 cells and primary microglia, where alterations of the Golgi ribbon were also observed. Silencing and overexpression experiments revealed that SPCA1 affects cell morphology, Golgi apparatus integrity, and phagocytic functions. Since SPCA1 is also an efficient Mn2+ transporter and considering that Mn2+ excess causes manganism in the brain, we addressed the role of microglial SPCA1 in Mn2+ toxicity. Our results revealed a clear effect of Mn2+ excess on the viability and morphology of microglia. Subcellular analysis showed Golgi fragmentation and subsequent alteration of SPCA1 distribution from early stages of toxicity. Removal of Mn2+ by washing improved the culture viability, although it did not effectively reverse Golgi fragmentation. Interestingly, pretreatment with curcumin maintained microglia cultures viable, prevented Mn2+ -induced Golgi fragmentation, and preserved SPCA Ca2+ -dependent activity, suggesting curcumin as a potential protective agent against Mn2+ -induced Golgi alterations in microglia., (© 2024 The Authors. GLIA published by Wiley Periodicals LLC.)- Published
- 2024
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40. Progression of myeloproliferative neoplasm with BCR::JAK2 fusion to acute leukemia of ambiguous lineage.
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Suárez EU, Piris MÁ, Rodríguez-Pinilla SM, García JF, López-Lorenzo JL, Cornago-Navascués J, Salgado-Sánchez R, Castaño-Bonilla T, Mata-Serna R, Alonso-Domínguez JM, and Llamas P
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- Humans, Janus Kinase 2 genetics, Mutation, Oncogene Proteins, Fusion genetics, Proto-Oncogene Proteins c-bcr genetics, Bone Marrow Neoplasms genetics, Leukemia, Myeloproliferative Disorders genetics
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- 2024
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41. Distribution of intracellular Ca 2+ -ATPases in the mouse retina and their involvement in light-induced cone degeneration.
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Martín-Oliva D, Martín-Guerrero SM, Carrasco MC, Neubrand VE, Martín-Estebané M, Marín-Teva JL, Navascués J, Cuadros MA, Vangheluwe P, and Sepúlveda MR
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- Animals, Mice, Adenosine Triphosphatases metabolism, Plasma Membrane Calcium-Transporting ATPases, Retina metabolism, Mammals metabolism, Calcium-Transporting ATPases genetics, Calcium-Transporting ATPases metabolism, Retinal Cone Photoreceptor Cells metabolism
- Abstract
Calcium signalling is involved in many processes in mammalian retina, from development to mature functions and neurodegeneration. Although proteins involved in Ca
2+ entry in retinal cells have been well studied, less is known about Ca2+ -clearance. Among the Ca2+ pumps, plasma membrane Ca2+ -ATPases (PMCAs) have been identified as key proteins extruding Ca2+ across the plasma membrane with specific distribution in developing and adult retina. However, the two main isoforms of intracellular Ca2+ -ATPases in the central nervous system, the sarco(endo)plasmic reticulum (ER) Ca2+ -ATPase 2b (SERCA2b) and the secretory pathway Ca2+ -ATPase 1 (SPCA1), which remove cytosolic Ca2+ into intracellular stores, have been less or not at all analysed, respectively. In this study, we described for the first time the SPCA1 localisation in adult mouse retina and we report differential distributions of SERCA2b and SPCA1 transporters within various classes of retinal neurons and distinct subcellular localisations. In addition, we studied the expression and localisation of both Ca2+ pumps in 661W cells, a cone photoreceptor-derived cell line. Since continuous exposure to high light intensity induces photodegeneration, we analysed the effect of LED light exposure on these cells and SERCA2b and SPCA1 distribution. We found that continuous mild LED-light exposure compromised cell survival and produced stress in the ER and Golgi, the Ca2+ stores where the two pumps are localised. These effects were reversed after halting light exposure and washing. This study demonstrates that Ca2+ signalling may be involved in light-induced photoreceptor cell damage and points to previously unrecognised functions of intracellular Ca2+ -ATPases in retina physiology., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2024
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42. Early outcomes from the Minimally Invasive Right Colectomy Anastomosis study (MIRCAST).
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Gómez Ruiz M, Espin-Basany E, Spinelli A, Cagigas Fernández C, Bollo Rodriguez J, María Enriquez Navascués J, Rautio T, and Tiskus M
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- Humans, Cohort Studies, Prospective Studies, Colectomy methods, Anastomosis, Surgical methods, Surgical Wound Infection epidemiology, Surgical Wound Infection etiology, Treatment Outcome, Retrospective Studies, Laparoscopy methods, Colonic Neoplasms surgery
- Abstract
Background: The impact of method of anastomosis and minimally invasive surgical technique on surgical and clinical outcomes after right hemicolectomy is uncertain. The aim of the MIRCAST study was to compare intracorporeal and extracorporeal anastomosis (ICA and ECA respectively), each using either a laparoscopic approach or robot-assisted surgery during right hemicolectomies for benign or malignant tumours., Methods: This was an international, multicentre, prospective, observational, monitored, non-randomized, parallel, four-cohort study (laparoscopic ECA; laparoscopic ICA; robot-assisted ECA; robot-assisted ICA). High-volume surgeons (at least 30 minimally invasive right colectomy procedures/year) from 59 hospitals across 12 European countries treated patients over a 3-year interval The primary composite endpoint was 30-day success, defined by two measures of efficacy-absence of surgical wound infection and of any major complication within the first 30 days after surgery. Secondary outcomes were: overall complications, conversion rate, duration of operation, and number of lymph nodes harvested. Propensity score analysis was used for comparison of ICA with ECA, and robot-assisted surgery with laparoscopy., Results: Some 1320 patients were included in an intention-to-treat analysis (laparoscopic ECA, 555; laparoscopic ICA, 356; robot-assisted ECA, 88; robot-assisted ICA, 321). No differences in the co-primary endpoint at 30 days after surgery were observed between cohorts (7.2 and 7.6 per cent in ECA and ICA groups respectively; 7.8 and 6.6 per cent in laparoscopic and robot-assisted groups). Lower overall complication rates were observed after ICA, specifically less ileus, and nausea and vomiting after robot-assisted procedures., Conclusion: No difference in the composite outcome of surgical wound infections and severe postoperative complications was found between intracorporeal versus extracorporeal anastomosis or laparoscopy versus robot-assisted surgery., (© The Author(s) 2023. Published by Oxford University Press on behalf of BJS Society Ltd.)
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- 2023
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43. LPS-stimulated microglial cells promote ganglion cell death in organotypic cultures of quail embryo retina.
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Sierra-Martín A, Navascués J, Neubrand VE, Sepúlveda MR, Martín-Oliva D, Cuadros MA, and Marín-Teva JL
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During development microglia colonize the central nervous system (CNS) and play an important role in programmed cell death, not only because of their ability to remove dead cells by phagocytosis, but also because they can promote the death of neuronal and glial cells. To study this process, we used as experimental systems the developing in situ quail embryo retina and organotypic cultures of quail embryo retina explants (QEREs). In both systems, immature microglia show an upregulation of certain inflammatory markers, e.g., inducible NO synthase (iNOS), and nitric oxide (NO) under basal conditions, which can be further enhanced with LPS-treatment. Hence, we investigated in the present study the role of microglia in promoting ganglion cell death during retinal development in QEREs. Results showed that LPS-stimulation of microglia in QEREs increases (i) the percentage of retinal cells with externalized phosphatidylserine, (ii) the frequency of phagocytic contacts between microglial and caspase-3-positive ganglion cells, (iii) cell death in the ganglion cell layer, and (iv) microglial production of reactive oxygen/nitrogen species, such as NO. Furthermore, iNOS inhibition by L-NMMA decreases cell death of ganglion cells and increases the number of ganglion cells in LPS-treated QEREs. These data demonstrate that LPS-stimulated microglia induce ganglion cell death in cultured QEREs by a NO-dependent mechanism. The fact that phagocytic contacts between microglial and caspase-3-positive ganglion cells increase suggests that this cell death might be mediated by microglial engulfment, although a phagocytosis-independent mechanism cannot be excluded., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Sierra-Martín, Navascués, Neubrand, Sepúlveda, Martín-Oliva, Cuadros and Marín-Teva.)
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- 2023
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44. ZBTB16-RARα-Positive Atypical Promyelocytic Leukemia: A Case Report.
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Pardo Gambarte L, Franganillo Suárez A, Cornago Navascués J, Soto de Ozaeta C, Blas López C, Atance Pasarisas M, Salgado Sánchez RN, Serrano Del Castillo C, Mata Serna R, Velasco Rodríguez D, López-Lorenzo JL, Llamas-Sillero P, and Solán Blanco L
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- Bone Marrow, Humans, Oncogene Proteins, Fusion genetics, Promyelocytic Leukemia Zinc Finger Protein genetics, Translocation, Genetic genetics, Tretinoin therapeutic use, Leukemia, Promyelocytic, Acute drug therapy, Leukemia, Promyelocytic, Acute genetics
- Abstract
Background: The majority of patients with acute promyelocytic leukemia (APL) manifest a specific chromosomal translocation t(15;17)(q22;q21), characterized by the fusion of RARA and PML genes. However, a proportion of APL cases are due to variant translocations, being t(11;17) (q23;q21) the most common amongst them. With the major exception of ZBTB16-RARA t(11;17) APL, these variant APL cases present similar morphological features as classic APL and are characterized by a lack of differentiation response to retinoids., Case Summary: We describe the case of variant APL with the ZBTB16-RARA fusion gene, showing a distinct morphology of classical APL, characterized by crystalline intracytoplasmic inclusions in both peripheral blood (PB) and bone marrow (BM) patients' blasts. Our patient was treated with two courses of intensive chemotherapy, initiating maintenance treatment with all-trans retinoic acid (ATRA) on day twenty-eight of the second course. Our patient achieved complete remission (CR) once the intensive chemotherapy was combined with ATRA., Conclusions: This is the second case described of APL with t(11;17) that showed crystalline intracytoplasmic inclusions. The finding of these morphological features may suggest the presence of a variant translocation with RARA, being that both cases described are related to the presence of t(11;17). Despite induction treatment with intensive chemotherapy that included a seven-day continuous treatment with cytarabine (200 mg/m
2 ), plus daily idarubicin (12 mg/m2 ) during the first three days, our patient did not achieve complete remission (CR) until scheduled 3 + 7 regimen combined with ATRA treatment was established. This observation suggests that ATRA may be partially effective in some ZBTB16-RARA APLs.- Published
- 2022
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45. Characteristics, clinical outcomes, and risk factors of SARS-COV-2 infection in adult acute myeloid leukemia patients: experience of the PETHEMA group.
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Palanques-Pastor T, Megías-Vericat JE, Martínez P, López Lorenzo JL, Cornago Navascués J, Rodriguez Macias G, Cano I, Arnan Sangerman M, Vidriales Vicente MB, Algarra Algarra JL, Foncillas MÁ, Herrera P, Botella Prieto C, Vives S, Figuera Álvarez Á, Cuevas Palomares L, Sobas M, Contento Gonzalo A, Cuello García R, Amutio Diez ME, De Miguel Llorente D, Navas Elorza B, Bergua Burgues JM, Bernal Del Castillo T, Mateos Rodríguez MC, de Cabo López E, Franco Villegas AC, García Boyero R, Escolano Escobar C, Seri Merino C, Cervero C, Roldán Pérez A, Hermosín Ramos L, Cervera Calvo M, Olave MT, Villafuerte Gutiérrez P, de Laiglesiai A, Serrano J, Najera Irazu MJ, Piñana JL, Sanz MÁ, Martínez-López J, and Montesinos P
- Subjects
- Adult, Humans, Lopinavir, Risk Factors, SARS-CoV-2, COVID-19, Leukemia, Myeloid, Acute epidemiology, Leukemia, Myeloid, Acute therapy
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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection produces higher morbidity and mortality in hematological malignancies, but evidence in acute myeloid leukemia (AML) is scarce. A multicenter observational study was conducted to determine the clinical outcomes and assess the impact of therapeutic approaches in adult AML patients with SARS-CoV-2 infection in the first wave (March-May 2020). Overall, 108 patients were included: 51.9% with active leukemia and 70.4% under therapeutic schedules for AML. Signs and symptoms of SARS-CoV-2 were present in 96.3% of patients and 82.4% received specific treatment for SARS-CoV-2. The mortality rate was 43.5% and was correlated with age, gender, active leukemia, dyspnea, severe SARS-CoV-2, intensive care measures, neutrophil count, and D-dimer levels. A protective effect was found with azithromycin, lopinavir/ritonavir, and normal liver enzyme levels. During the SARS-CoV-2 first wave, our findings suggested an increased mortality in AML in a short period. SARS-CoV-2 management could be guided by risk factors in AML patients.
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- 2021
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46. The endoplasmic reticulum Ca 2+ -ATPase SERCA2b is upregulated in activated microglia and its inhibition causes opposite effects on migration and phagocytosis.
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Morales-Ropero JM, Arroyo-Urea S, Neubrand VE, Martín-Oliva D, Marín-Teva JL, Cuadros MA, Vangheluwe P, Navascués J, Mata AM, and Sepúlveda MR
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- Animals, Calcium metabolism, Endoplasmic Reticulum metabolism, Lipopolysaccharides toxicity, Mice, Phagocytosis, Thapsigargin pharmacology, Microglia metabolism, Sarcoplasmic Reticulum Calcium-Transporting ATPases genetics, Sarcoplasmic Reticulum Calcium-Transporting ATPases metabolism
- Abstract
Activation of microglia is an early immune response to damage in the brain. Although a key role for Ca
2+ as trigger of microglial activation has been considered, little is known about the molecular scenario for regulating Ca2+ homeostasis in these cells. Taking into account the importance of the endoplasmic reticulum as a cellular Ca2+ store, the sarco(endo)plasmic reticulum Ca2+ -ATPase (SERCA2b) is an interesting target to modulate intracellular Ca2+ dynamics. We found upregulation of SERCA2b in activated microglia of human brain with Alzheimer's disease and we further studied the participation of SERCA2b in microglial functions by using the BV2 murine microglial cell line and primary microglia isolated from mouse brain. To trigger microglia activation, we used the bacterial lipopolysaccharide (LPS), which is known to induce an increase of cytosolic Ca2+ . Our results showed an upregulated expression of SERCA2b in LPS-induced activated microglia likely associated to an attempt to restore the increased cytosolic Ca2+ concentration. We analyzed SERCA2b contribution in microglial migration by using the specific SERCA inhibitor thapsigargin in scratch assays. Microglial migration was strongly stimulated with thapsigargin, even more than with LPS-induction, but delayed in time. However, phagocytic capacity of microglia was blocked in the presence of the SERCA inhibitor, indicating the importance of a tight control of cytosolic Ca2+ in these processes. All together, these results provide for the first time compelling evidence for SERCA2b as a major player regulating microglial functions, affecting migration and phagocytosis in an opposite manner., (© 2020 Wiley Periodicals LLC.)- Published
- 2021
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47. PARP-1 activation after oxidative insult promotes energy stress-dependent phosphorylation of YAP1 and reduces cell viability.
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Martín-Guerrero SM, Casado P, Hijazi M, Rajeeve V, Plaza-Díaz J, Abadía-Molina F, Navascués J, Cuadros MA, Cutillas PR, and Martín-Oliva D
- Subjects
- AMP-Activated Protein Kinases genetics, AMP-Activated Protein Kinases metabolism, Adaptor Proteins, Signal Transducing genetics, Cell Survival drug effects, Cell Survival genetics, HeLa Cells, Humans, Hydrogen Peroxide pharmacology, NAD genetics, NAD metabolism, Phosphorylation drug effects, Phosphorylation genetics, Poly (ADP-Ribose) Polymerase-1 genetics, Transcription Factors genetics, YAP-Signaling Proteins, Adaptor Proteins, Signal Transducing metabolism, Oxidative Stress, Poly (ADP-Ribose) Polymerase-1 metabolism, Transcription Factors metabolism
- Abstract
Poly(ADP-ribose) polymerase 1 (PARP-1) is a nuclear enzyme that catalyze the transfer of ADP-ribose units from NAD+ to several target proteins involved in cellular stress responses. Using WRL68 (HeLa derivate) cells, we previously showed that PARP-1 activation induced by oxidative stress after H2O2 treatment lead to depletion of cellular NAD+ and ATP, which promoted cell death. In this work, LC-MS/MS-based phosphoproteomics in WRL68 cells showed that the oxidative damage induced by H2O2 increased the phosphorylation of YAP1, a transcriptional co-activator involved in cell survival, and modified the phosphorylation of other proteins involved in transcription. Genetic or pharmacological inhibition of PARP-1 in H2O2-treated cells reduced YAP1 phosphorylation and degradation and increased cell viability. YAP1 silencing abrogated the protective effect of PARP-1 inhibition, indicating that YAP1 is important for the survival of WRL68 cells exposed to oxidative damage. Supplementation of NAD+ also reduced YAP1 phosphorylation, suggesting that the loss of cellular NAD+ caused by PARP-1 activation after oxidative treatment is responsible for the phosphorylation of YAP1. Finally, PARP-1 silencing after oxidative treatment diminished the activation of the metabolic sensor AMPK. Since NAD+ supplementation reduced the phosphorylation of some AMPK substrates, we hypothesized that the loss of cellular NAD+ after PARP-1 activation may induce an energy stress that activates AMPK. In summary, we showed a new crucial role of PARP-1 in the response to oxidative stress in which PARP-1 activation reduced cell viability by promoting the phosphorylation and degradation of YAP1 through a mechanism that involves the depletion of NAD+., (© 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.)
- Published
- 2020
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48. PLZF-RAR α , NPM1-RAR α , and Other Acute Promyelocytic Leukemia Variants: The PETHEMA Registry Experience and Systematic Literature Review.
- Author
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Sobas M, Talarn-Forcadell MC, Martínez-Cuadrón D, Escoda L, García-Pérez MJ, Mariz J, Mela-Osorio MJ, Fernández I, Alonso-Domínguez JM, Cornago-Navascués J, Rodríguez-Macias G, Amutio ME, Rodríguez-Medina C, Esteve J, Sokół A, Murciano-Carrillo T, Calasanz MJ, Barrios M, Barragán E, Sanz MA, and Montesinos P
- Abstract
It has been suggested that 1-2% of acute promyelocytic leukemia (APL) patients present variant rearrangements of retinoic acid receptor alpha (RARα) fusion gene, with the promyelocytic leukaemia zinc finger (PLZF)/RAR
α being the most frequent. Resistance to all-trans-retinoic acid (ATRA) and arsenic trioxide (ATO) has been suggested in PLZF/RARα and other variant APLs. Herein, we analyze the incidence, characteristics, and outcomes of variant APLs reported to the multinational PETHEMA (Programa para el Tratamiento de Hemopatias Malignas) registry, and we perform a systematic review in order to shed light on strategies to improve management of these extremely rare diseases. Of 2895 patients with genetically confirmed APL in the PETHEMA registry, 11 had variant APL (0.4%) (9 PLZF-RARα and 2 NPM1-RARα ), 9 were men, with median age of 44.6 years (3 months to 76 years), median leucocytes (WBC) 16.8 × 109 /L, and frequent coagulopathy. Eight patients were treated with ATRA plus chemotherapy-based regimens, and 3 with chemotherapy-based. As compared to previous reports, complete remission and survival was slightly better in our cohort, with 73% complete remission (CR) and 73% survival despite a high relapse rate (43%). After analyzing our series and performing a comprehensive and critical review of the literature, strong recommendations on appropriate management of variant APL are not possible due to the low number and heterogeneity of patients reported so far.- Published
- 2020
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49. Poly(ADP-Ribose) Polymerase-1 inhibition potentiates cell death and phosphorylation of DNA damage response proteins in oxidative stressed retinal cells.
- Author
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Martín-Guerrero SM, Casado P, Muñoz-Gámez JA, Carrasco MC, Navascués J, Cuadros MA, López-Giménez JF, Cutillas PR, and Martín-Oliva D
- Subjects
- Animals, Ataxia Telangiectasia Mutated Proteins metabolism, Blotting, Western, Caspase 3 metabolism, Cell Death, Cell Line, DNA-Binding Proteins, Electrophoresis, Polyacrylamide Gel, Histones metabolism, Hydrogen Peroxide toxicity, In Situ Nick-End Labeling, Mice, Mice, Inbred C57BL, Oxidants toxicity, Phenanthrenes pharmacology, Phosphoproteins metabolism, Phosphorylation, Poly (ADP-Ribose) Polymerase-1 metabolism, Poly(ADP-ribose) Polymerase Inhibitors pharmacology, Retina metabolism, DNA Damage, Eye Proteins metabolism, Oxidative Stress, Poly (ADP-Ribose) Polymerase-1 antagonists & inhibitors, Retina pathology
- Abstract
Oxidative stress (OxS) is involved in the development of cell injures occurring in retinal diseases while Poly(ADP-ribose) Polymerase-1 (PARP-1) is a key protein involved in the repair of the DNA damage caused by OxS. Inhibition of PARP-1 activity with the pharmacological inhibitor PJ34 in mouse retinal explants subjected to H
2 O2 -induced oxidative damage resulted in an increase of apoptotic cells. Reduction of cell growth was also observed in the mouse cone like cell line 661 W in the presence of PJ34 under OxS conditions. Mass spectrometry-based phosphoproteomics analysis performed in 661 W cells determined that OxS induced significant changes in the phosphorylation in 1807 of the 8131 peptides initially detected. Blockade of PARP-1 activity after the oxidative treatment additionally increased the phosphorylation of multiple proteins, many of them at SQ motifs and related to the DNA-damage response (DDR). These motifs are substrates of the kinases ATM/ATR, which play a central role in DDR. Western blot analysis confirmed that the ATM/ATR activity measured and the phosphorylation at SQ motifs of ATM/ATR substrates was augmented when PARP-1 activity was inhibited under OxS conditions, in 661 W cells. Phosphorylation of ATM/ATR substrates, including the phosphorylation of the histone H2AX were also induced in organotypic cultures of retinal explants subjected to PARP-1 inhibition during exposure to OxS. In conclusion, inhibition of PARP-1 increased the phosphorylation and hence the activation of several proteins involved in the response to DNA damage, like the ATM protein kinase. This finally resulted in an augmented injury in mouse retinal cells suffering from OxS. Therefore, the inhibition of PARP-1 activity may have a negative outcome in the treatment of retinal diseases in which OxS is involved., (Copyright © 2019 Elsevier Ltd. All rights reserved.)- Published
- 2019
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50. Poly(ADP-ribose)polymerases inhibitors prevent early mitochondrial fragmentation and hepatocyte cell death induced by H2O2.
- Author
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Martín-Guerrero SM, Muñoz-Gámez JA, Carrasco MC, Salmerón J, Martín-Estebané M, Cuadros MA, Navascués J, and Martín-Oliva D
- Subjects
- Cell Line, Hepatocytes cytology, Humans, Reactive Oxygen Species metabolism, Hepatocytes drug effects, Hydrogen Peroxide pharmacology, Mitochondria drug effects, Poly(ADP-ribose) Polymerase Inhibitors pharmacology
- Abstract
Poly(ADP-ribose)polymerases (PARPs) are a family of NAD+ consuming enzymes that play a crucial role in many cellular processes, most clearly in maintaining genome integrity. Here, we present an extensive analysis of the alteration of mitochondrial morphology and the relationship to PARPs activity after oxidative stress using an in vitro model of human hepatic cells. The following outcomes were observed: reactive oxygen species (ROS) induced by oxidative treatment quickly stimulated PARPs activation, promoted changes in mitochondrial morphology associated with early mitochondrial fragmentation and energy dysfunction and finally triggered apoptotic cell death. Pharmacological treatment with specific PARP-1 (the major NAD+ consuming poly(ADP-ribose)polymerases) and PARP-1/PARP-2 inhibitors after the oxidant insult recovered normal mitochondrial morphology and, hence, increased the viability of human hepatic cells. As the PARP-1 and PARP-1/PARP-2 inhibitors achieved similar outcomes, we conclude that most of the PARPs effects were due to PARP-1 activation. NAD+ supplementation had similar effects to those of the PARPs inhibitors. Therefore, PARPs activation and the subsequent NAD+ depletion are crucial events in decreased cell survival (and increased apoptosis) in hepatic cells subjected to oxidative stress. These results suggest that the alterations in mitochondrial morphology and function seem to be related to NAD+ depletion, and show for the first time that PARPs inhibition abrogates mitochondrial fragmentation. In conclusion, the inhibition of PARPs may be a valuable therapeutic approach for treating liver diseases, by reducing the cell death associated with oxidative stress.
- Published
- 2017
- Full Text
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