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27 results on '"Naumann, Annette"'

9. Downregulation of Cinnamoyl-Coenzyme A Reductase in Poplar: Multiple-Level Phenotyping Reveals Effects on Cell Wall Polymer Metabolism and Structure

Author

Leplé, Jean-Charles, Dauwe, Rebecca, Morreel, Kris, Storme, Véronique, Lapierre, Catherine, Pollet, Brigitte, Naumann, Annette, Kang, Kyu-Young, Kim, Hoon, Ruel, Katia, Lefèbvre, Andrée, Joseleau, Jean-Paul, Grima-Pettenati, Jacqueline, De Rycke, Riet, Andersson-Gunnerås, Sara, Erban, Alexander, Fehrle, Ines, Petit-Conil, Michel, Kopka, Joachim, Polle, Andrea, Messens, Eric, Sundberg, Björn, Mansfield, Shawn D., Ralph, John, Pilate, Gilles, and Boerjan, Wout

Published
2007
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13. MOESM1 of Characterization of essential oil distribution in the root cross-section of Valeriana officinalis L. s.l. by using histological imaging techniques

Author

Penzkofer, Michael, Baron, Andrea, Naumann, Annette, KräHmer, Andrea, Schulz, Hartwig, and Heuberger, Heidi

Subjects
ComputerApplications_COMPUTERSINOTHERSYSTEMS
Abstract

Additional file 1. Analyzation steps and software macros for localization of the oil droplets, root-slice area and edges, as well as the root-slice center.

Published
2018
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14. Ectomycorrhizal identification in environmental samples of tree roots by Fourier-transform infrared (FTIR) spectroscopy

Author

Pena, Rodica, Lang, Christa, Naumann, Annette, and Polle, Andrea

Subjects
deciduous forests, field samples, soilbornefungi, mycorrhiza, deciduousforests, infraredspectroscopy, clusteranalysis, fieldsamples, Plant Science, Original Research Article, soilborne fungi, infrared spectroscopy, cluster analysis
Abstract

Roots of forest trees are associated with various ectomycorrhizal (ECM) fungal species that are involved in nutrient exchange between host plant and the soil compartment. The identification of ECM fungi in small environmental samples is difficult. The present study tested the feasibility of attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy followed by hierarchical cluster analysis (HCA) to discriminate in situ collected ECM fungal species. Root tips colonized by distinct ECM fungal species, i.e., Amanita rubescens, Cenococcum geophilum, Lactarius subdulcis, Russula ochroleuca, and Xerocomus pruinatus were collected in mono-specific beech (Fagus sylvatica) and mixed deciduous forests in different geographic areas to investigate the environmental variability of the ECM FTIR signatures. A clear HCA discrimination was obtained for ECM fungal species independent of individual provenance. Environmental variability neither limited the discrimination between fungal species nor provided sufficient resolution to discern species sub-clusters for different sites. However, the de-convoluted FTIR spectra contained site-related spectral information for fungi with wide nutrient ranges, but not for Lactarius subdulcis, a fungus residing only in the litter layer. Specific markers for distinct ECM were identified in spectral regions associated with carbohydrates (i.e., mannans), lipids, and secondary protein structures. The present results support that FTIR spectroscopy coupled with multivariate analysis is a reliable and fast method to identify ECM fungal species in minute environmental samples. Moreover, our data suggest that the FTIR spectral signatures contain information on physiological and functional traits of ECM fungi. Open-Access-Publikationsfonds 2014 peerReviewed

Published
2014

27. Characterization of essential oil distribution in the root cross-section of <italic>Valeriana officinalis</italic> L. s.l. by using histological imaging techniques.

Author

Penzkofer, Michael, Baron, Andrea, Naumann, Annette, Krähmer, Andrea, Schulz, Hartwig, and Heuberger, Heidi

Subjects
ESSENTIAL oils, VALERIANA officinalis, VEGETABLE oils, PLANT genetics, CULTIVARS
Abstract

Background: The essential oil is an important compound of the root and rhizome of medicinally used valerian (Valeriana officinalis L. s.l.), with a stated minimum content in the European pharmacopoeia. The essential oil is located in droplets, of which the position and distribution in the total root cross-section of different valerian varieties, root thicknesses and root horizons are determined in this study using an adapted fluorescence-microscopy and automatic imaging analysis method. The study was initiated by the following facts: A probable negative correlation between essential oil content and root thickness in selected single plants (elites), observed during the breeding of coarsely rooted valerian with high oil content. Higher essential oil content after careful hand-harvest and processing of the roots. Results: In preliminary tests, the existence of oil containing droplets in the outer and inner regions of the valerian roots was confirmed by histological techniques and light-microscopy, as well as Fourier-transform infrared spectroscopy. Based on this, fluorescence-microscopy followed by image analysis of entire root cross-sections, showed that a large number of oil droplets (on average 43% of total oil droplets) are located close to the root surface. The remaining oil droplets are located in the inner regions (parenchyma) and showed varying density gradients from the inner to the outer regions depending on genotype, root thickness and harvesting depth. Conclusions: Fluorescence-microscopy is suitable to evaluate prevalence and distribution of essential oil droplets of valerian in entire root cross-sections. The oil droplet density gradient varies among genotypes. Genotypes with a linear rather than an exponential increase of oil droplet density from the inner to the outer parenchyma can be chosen for better stability during post-harvest processing. The negative correlation of essential oil content and root thickness as observed in our breeding material can be counteracted through a selection towards generally high oil droplet density levels, and large oil droplet sizes independent of root thickness. [ABSTRACT FROM AUTHOR]

Published
2018
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