10 results on '"Nattamai K"'
Search Results
2. Human pluripotent stem cell-derived organoids repair damaged bowel in vivo.
- Author
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Poling HM, Sundaram N, Fisher GW, Singh A, Shiley JR, Nattamai K, Govindarajah V, Cortez AR, Krutko MO, Ménoret S, Anegon I, Kasendra M, Wells JM, Mayhew CN, Takebe T, Mahe MM, and Helmrath MA
- Subjects
- Humans, Animals, Intestine, Small cytology, Mice, Regeneration, Rats, Organoids, Pluripotent Stem Cells cytology, Pluripotent Stem Cells metabolism
- Abstract
The fundamental goal of tissue engineering is to functionally restore or improve damaged tissues or organs. Here we address this in the small bowel using an in vivo xenograft preclinical acute damage model. We investigated the therapeutic capacity of human intestinal organoids (HIOs), which are generated from human pluripotent stem cells (hPSCs), to repair damaged small bowel. We hypothesized that the HIO's cellular complexity would allow it to sustain transmural engraftment. To test this, we developed a rodent injury model where, through luminal delivery, we demonstrated that fragmented HIOs engraft, proliferate, and persist throughout the bowel following repair. Not only was restitution of the mucosal layer observed, but significant incorporation was also observed in the muscularis and vascular endothelium. Further analysis characterized sustained cell type presence within the regenerated regions, retention of proximal regionalization, and the neo-epithelia's function. These findings demonstrate the therapeutic importance of mesenchyme for intestinal injury repair., Competing Interests: Declaration of interests CCHMC has a patent application in process related to the work in this study., (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Published
- 2024
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3. Latexin deletion protects against radiation-induced hematopoietic damages via selective activation of Bcl-2 prosurvival pathway.
- Author
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Zhang C, Cui X, Liu Y, Wang F, Signer R, Nattamai K, Zhou D, Zheng Y, Geiger H, Wan F, and Liang Y
- Subjects
- Humans, Apoptosis
- Published
- 2023
- Full Text
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4. A critical role of RUNX1 in governing megakaryocyte-primed hematopoietic stem cell differentiation.
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Wang C, Tu Z, Cai X, Wang W, Davis AK, Nattamai K, Paranjpe A, Dexheimer P, Wu J, Huang FL, Geiger H, Huang G, and Zheng Y
- Subjects
- Animals, Mice, Hematopoietic Stem Cells metabolism, Hematopoiesis, Cell Differentiation genetics, Megakaryocytes metabolism, Core Binding Factor Alpha 2 Subunit genetics, Core Binding Factor Alpha 2 Subunit metabolism
- Abstract
As a transcription factor in the RUNT domain core-binding factor family, RUNX1 is crucial in multiple stages of hematopoiesis, and its mutation can cause familial platelet disorder with a predisposition to acute myeloid leukemia. Previous work has established that RUNX1 is involved in the maturation of megakaryocytes (MKs) and the production of platelets. Recent studies have shown that there exists a subpopulation of hematopoietic stem cells (HSCs) with relatively high expression of von Willebrand factor and CD41 at the apex of the HSC hierarchy, termed MK-HSCs, which can give rise to MKs without going through the traditional differentiation trajectory from HSC via MPP (multipotent progenitors) and MEP (megakaryocyte-erythroid progenitor). Here, by using Runx1F/FMx1-Cre mouse model, we discovered that the MK-HSC to MK direct differentiation can occur within 1 cell division, and RUNX1 is an important regulator in the process. Runx1 knockout results in a drastic decrease in platelet counts and a severe defect in the differentiation from MK-HSCs to MKs. Single cell RNA sequencing (RNAseq) analysis shows that MK-HSCs have a distinct gene expression signature compared with non-MK-HSCs, and Runx1 deletion alters the platelet and MK-related gene expression in MK-HSCs. Furthermore, bulk RNAseq and Cut&Run analyses show that RUNX1 binds to multiple essential MK or platelet developmental genes, such as Spi1, Selp, and Itga2b and regulates their expressions in MK-HSCs. Thus, by modulating the expression of MK-related genes, RUNX1 governs the direct differentiation from MK-HSCs to MKs and platelets., (© 2023 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.)
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- 2023
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5. A Wnt5a-Cdc42 axis controls aging and rejuvenation of hair-follicle stem cells.
- Author
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Tiwari RL, Mishra P, Martin N, George NO, Sakk V, Soller K, Nalapareddy K, Nattamai K, Scharffetter-Kochanek K, Florian MC, and Geiger H
- Subjects
- Animals, Cellular Senescence physiology, Mice, Hair Follicle growth & development, Rejuvenation physiology, Stem Cells metabolism, Wnt Signaling Pathway, Wnt-5a Protein genetics
- Abstract
Normal hair growth occurs in cycles, comprising growth (anagen), cessation (catagen) and rest (telogen). Upon aging, the initiation of anagen is significantly delayed, which results in impaired hair regeneration. Hair regeneration is driven by hair follicle stem cells (HFSCs). We show here that aged HFSCs present with a decrease in canonical Wnt signaling and a shift towards non-canonical Wnt5a driven signaling which antagonizes canonical Wnt signaling. Elevated expression of Wnt5a in HFSCs upon aging results in elevated activity of the small RhoGTPase Cdc42 as well as a change in the spatial distribution of Cdc42 within HFSCs. Treatment of aged HFSC with a specific pharmacological inhibitor of Cdc42 activity termed CASIN to suppress the aging-associated elevated activity of Cdc42 restored canonical Wnt signaling in aged HFSCs. Treatment of aged mice in vivo with CASIN induced anagen onset and increased the percentage of anagen skin areas. Aging-associated functional deficits of HFSCs are at least in part intrinsic to HFSCs and can be restored by rational pharmacological approaches.
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- 2021
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6. FOXO activity adaptation safeguards the hematopoietic stem cell compartment in hyperglycemia.
- Author
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Govindarajah V, Lee JM, Solomon M, Goddard B, Nayak R, Nattamai K, Geiger H, Salomonis N, Cancelas JA, and Reynaud D
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- Animals, DNA Damage, Mice, Oxidative Stress, Signal Transduction, Hematopoietic Stem Cells, Hyperglycemia
- Abstract
Hematopoietic stem cell (HSC) activity is tightly controlled to ensure the integrity of the hematopoietic system during the organism's lifetime. How the HSC compartment maintains its long-term fitness in conditions of chronic stresses associated with systemic metabolic disorders is poorly understood. In this study, we show that obesity functionally affects the long-term function of the most immature engrafting HSC subpopulation. We link this altered regenerative activity to the oxidative stress and the aberrant constitutive activation of the AKT signaling pathway that characterized the obese environment. In contrast, we found minor disruptions of the HSC function in obese mice at steady state, suggesting that active mechanisms could protect the HSC compartment from its disturbed environment. Consistent with this idea, we found that FOXO proteins in HSCs isolated from obese mice become insensitive to their normal upstream regulators such as AKT, even during intense oxidative stress. We established that hyperglycemia, a key condition associated with obesity, is directly responsible for the alteration of the AKT-FOXO axis in HSCs and their abnormal oxidative stress response. As a consequence, we observed that HSCs isolated from a hyperglycemic environment display enhanced resistance to oxidative stress and DNA damage. Altogether, these results indicate that chronic metabolic stresses associated with obesity and/or hyperglycemia affect the wiring of the HSCs and modify their oxidative stress response. These data suggest that the uncoupling of FOXO from its environmental regulators could be a key adaptive strategy that promotes the survival of the HSC compartment in obesity., (© 2020 by The American Society of Hematology.)
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- 2020
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7. Loss of DEK induces radioresistance of murine restricted hematopoietic progenitors.
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Serrano-Lopez J, Nattamai K, Pease NA, Shephard MS, Wellendorf AM, Sertorio M, Smith EA, Geiger H, Wells SI, Cancelas JA, and Privette Vinnedge LM
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- Animals, Hematopoietic Stem Cells cytology, Mice, Mice, Knockout, DNA Damage, DNA-Binding Proteins deficiency, Hematopoiesis physiology, Hematopoietic Stem Cells metabolism, Oncogene Proteins deficiency, Poly-ADP-Ribose Binding Proteins deficiency, Radiation Tolerance physiology
- Abstract
Self-renewing hematopoietic stem cells and multipotent progenitor cells are responsible for maintaining hematopoiesis throughout an individual's lifetime. For overall health and survival, it is critical that the genome stability of these cells is maintained and that the cell population is not exhausted. Previous reports have indicated that the DEK protein, a chromatin structural protein that functions in numerous nuclear processes, is required for DNA damage repair in vitro and long-term engraftment of hematopoietic stem cells in vivo. Therefore, we investigated the role of DEK in normal hematopoiesis and response to DNA damaging agents in vivo. Here, we report that hematopoiesis is largely unperturbed in DEK knockout mice compared with wild-type (WT) controls. However, DEK knockout mice have fewer radioprotective units, but increased capacity to survive repeated sublethal doses of radiation exposure compared with WT mice. Furthermore, this increased survival correlated with a sustained quiescent state in which DEK knockout restricted hematopoietic progenitor cells (HPC-1) were nearly three times more likely to be quiescent following irradiation compared with WT cells and were significantly more radioresistant during the early phases of myeloid reconstitution. Together, our studies indicate that DEK functions in the normal hematopoietic stress response to recurrent radiation exposure., (Copyright © 2018 ISEH – Society for Hematology and Stem Cells. Published by Elsevier Inc. All rights reserved.)
- Published
- 2018
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8. The Spindle Assembly Checkpoint Is Required for Hematopoietic Progenitor Cell Engraftment.
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Brown A, Pospiech J, Eiwen K, Baker DJ, Moehrle B, Sakk V, Nattamai K, Vogel M, Grigoryan A, and Geiger H
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- Animals, Antimitotic Agents pharmacology, Apoptosis, Cells, Cultured, Hematopoiesis, Hematopoietic Stem Cells drug effects, Hematopoietic Stem Cells metabolism, Mice, Mice, Inbred C57BL, Tumor Suppressor Protein p53 genetics, Tumor Suppressor Protein p53 metabolism, Hematopoietic Stem Cells cytology, M Phase Cell Cycle Checkpoints
- Abstract
The spindle assembly checkpoint plays a pivotal role in preventing aneuploidy and transformation. Many studies demonstrate impairment of this checkpoint in cancer cells. While leukemia is frequently driven by transformed hematopoietic stem and progenitor cells (HSPCs), the biology of the spindle assembly checkpoint in such primary cells is not very well understood. Here, we reveal that the checkpoint is fully functional in murine progenitor cells and, to a lesser extent, in hematopoietic stem cells. We show that HSPCs arrest at prometaphase and induce p53-dependent apoptosis upon prolonged treatment with anti-mitotic drugs. Moreover, the checkpoint can be chemically and genetically abrogated, leading to premature exit from mitosis, subsequent enforced G1 arrest, and enhanced levels of chromosomal damage. We finally demonstrate that, upon checkpoint abrogation in HSPCs, hematopoiesis is impaired, manifested by loss of differentiation potential and engraftment ability, indicating a critical role of this checkpoint in HSPCs and hematopoiesis., (Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2017
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9. Stem Cell-Specific Mechanisms Ensure Genomic Fidelity within HSCs and upon Aging of HSCs.
- Author
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Moehrle BM, Nattamai K, Brown A, Florian MC, Ryan M, Vogel M, Bliederhaeuser C, Soller K, Prows DR, Abdollahi A, Schleimer D, Walter D, Milsom MD, Stambrook P, Porteus M, and Geiger H
- Subjects
- Amino Acid Sequence, Animals, Apoptosis radiation effects, Bone Marrow Cells cytology, Bone Marrow Transplantation, Cells, Cultured, Checkpoint Kinase 2 genetics, Checkpoint Kinase 2 metabolism, DNA Damage radiation effects, Endodeoxyribonucleases genetics, Endodeoxyribonucleases metabolism, G1 Phase Cell Cycle Checkpoints radiation effects, Gamma Rays, Hematopoietic Stem Cells cytology, Loss of Heterozygosity, Mice, Mice, Inbred C57BL, Mutation, S Phase Cell Cycle Checkpoints radiation effects, Transplantation, Homologous, Whole-Body Irradiation, Aging, Genome, Hematopoietic Stem Cells metabolism
- Abstract
Whether aged hematopoietic stem and progenitor cells (HSPCs) have impaired DNA damage repair is controversial. Using a combination of DNA mutation indicator assays, we observe a 2- to 3-fold increase in the number of DNA mutations in the hematopoietic system upon aging. Young and aged hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) do not show an increase in mutation upon irradiation-induced DNA damage repair, and young and aged HSPCs respond very similarly to DNA damage with respect to cell-cycle checkpoint activation and apoptosis. Both young and aged HSPCs show impaired activation of the DNA-damage-induced G1-S checkpoint. Induction of chronic DNA double-strand breaks by zinc-finger nucleases suggests that HSPCs undergo apoptosis rather than faulty repair. These data reveal a protective mechanism in both the young and aged hematopoietic system against accumulation of mutations in response to DNA damage., (Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2015
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10. Reciprocal relationship between O6-methylguanine-DNA methyltransferase P140K expression level and chemoprotection of hematopoietic stem cells.
- Author
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Milsom MD, Jerabek-Willemsen M, Harris CE, Schambach A, Broun E, Bailey J, Jansen M, Schleimer D, Nattamai K, Wilhelm J, Watson A, Geiger H, Margison GP, Moritz T, Baum C, Thomale J, and Williams DA
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- Animals, Comet Assay, Fluorescent Antibody Technique, Genetic Vectors, Mice, Mice, Inbred C57BL, Retroviridae genetics, Transduction, Genetic, Hematopoietic Stem Cells enzymology, O(6)-Methylguanine-DNA Methyltransferase metabolism
- Abstract
Retroviral-mediated delivery of the P140K mutant O(6)-methylguanine-DNA methyltransferase (MGMT(P140K)) into hematopoietic stem cells (HSC) has been proposed as a means to protect against dose-limiting myelosuppressive toxicity ensuing from chemotherapy combining O(6)-alkylating agents (e.g., temozolomide) with pseudosubstrate inhibitors (such as O(6)-benzylguanine) of endogenous MGMT. Because detoxification of O(6)-alkylguanine adducts by MGMT is stoichiometric, it has been suggested that higher levels of MGMT will afford better protection to gene-modified HSC. However, accomplishing this goal would potentially be in conflict with current efforts in the gene therapy field, which aim to incorporate weaker enhancer elements to avoid insertional mutagenesis. Using a panel of self-inactivating gamma-retroviral vectors that express a range of MGMT(P140K) activity, we show that MGMT(P140K) expression by weaker cellular promoter/enhancers is sufficient for in vivo protection/selection following treatment with O(6)-benzylguanine/temozolomide. Conversely, the highest level of MGMT(P140K) activity did not promote efficient in vivo protection despite mediating detoxification of O(6)-alkylguanine adducts. Moreover, very high expression of MGMT(P140K) was associated with a competitive repopulation defect in HSC. Mechanistically, we show a defect in cellular proliferation associated with elevated expression of MGMT(P140K), but not wild-type MGMT. This proliferation defect correlated with increased localization of MGMT(P140K) to the nucleus/chromatin. These data show that very high expression of MGMT(P140K) has a deleterious effect on cellular proliferation, engraftment, and chemoprotection. These studies have direct translational relevance to ongoing clinical gene therapy studies using MGMT(P140K), whereas the novel mechanistic findings are relevant to the basic understanding of DNA repair by MGMT.
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- 2008
- Full Text
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