1. Role of the nucleocapsid region in HIV-1 Gag assembly as investigated by quantitative fluorescence-based microscopy
- Author
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Pascal Didier, Ludovic Richert, Jean-Luc Darlix, Salah Edin El Meshri, Yves Mély, Hugues de Rocquigny, de Rocquigny, Hugues, Targeting the HIV-1 Nucleocapsid Protein to fight Antiretroviral Drug Resistance - THINPAD - - EC:FP7:HEALTH2013-09-01 - 2016-08-31 - 601969 - VALID, Laboratoire de Biophotonique et Pharmacologie - UMR 7213 (LBP), Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS), Thanks are due to Institut National sur la Santé Et la Reherche Médicale (INSERM), Centre National Recherche Scientifique (CNRS), Agence National Recherche sur le SIDA (ANRS) (2012-14. CSS2), SIDACTION (AI22-1-01963) and Unistra for constant support. Thiswork was also supported by the European Project THINPAD 'Tar-geting the HIV-1 Nucleocapsid Protein to fight Antiretroviral Drug Resistance' (FP7 – grant agreement 601969)., and European Project: 601969,EC:FP7:HEALTH,FP7-HEALTH-2013-INNOVATION-2,THINPAD(2013)
- Subjects
Cancer Research ,viruses ,[SDV]Life Sciences [q-bio] ,Assembly ,Morphogenesis ,Biology ,Matrix (biology) ,gag Gene Products, Human Immunodeficiency Virus ,Virus ,Fluorescence ,Virology ,Quantitative fluorescence ,Hiv 1 gag ,Microscopy ,Animals ,Humans ,FRET-FLIM ,Nucleocapsid ,Gag ,Virus Assembly ,Cell Membrane ,HIV ,Nucleocapsid Proteins ,Rous sarcoma virus ,3. Good health ,Cell biology ,[SDV] Life Sciences [q-bio] ,Protein Transport ,Infectious Diseases ,Microscopy, Fluorescence ,Capsid ,HIV-1 ,Protein Multimerization ,NC ,Protein Binding - Abstract
International audience; The Gag precursor of HIV-1, formed of the four proteic regions matrix (MA), capsid (CA), nucleocapsid (NC) and p6, orchestrates virus morphogenesis. This complex process relies on three major interactions, NC-RNA acting as a scaffold, CA-CA and MA-membrane that targets assembly to the plasma membrane (PM). The characterization of the molecular mechanism of retroviral assembly has extensively benefited from biochemical studies and more recently an important step forward was achieved with the use of fluorescence-based techniques and fluorescently labeled viral proteins. In this review, we summarize the findings obtained with such techniques, notably quantitative-based approaches, which highlight the role of the NC region in Gag assembly.
- Published
- 2014
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