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1. Efficacy of Kamona strain Deladenus siricidicola nematodes for biological control of Sirex noctilio in North America and hybridisation with invasive conspecifics

Author

Tonya D. Bittner, Nathan Havill, Isis A.L. Caetano, and Ann E. Hajek

Subjects
Biology (General), QH301-705.5
Abstract

Sirex noctilio is an invasive woodwasp that, along with its symbiotic fungus, has killed pine trees (Pinus spp.) in North America and in numerous countries in the Southern Hemisphere. We tested a biological control agent in North America that has successfully controlled S. noctilio in Oceania, South Africa, and South America. Deladenus siricidicola nematodes feed on the symbiotic white rot fungus Amylostereum areolatum and can switch to being parasitic on S. noctilio. When parasitic, the Kamona nematode strain can sterilise the eggs of S. noctilio females. However, in North America, a different strain of D. siricidicola (NA), presumably introduced along with the woodwasp, parasitises but does not sterilise S. noctilio. We tested the sterilising Kamona biological control strain of D. siricidicola against S. noctilio in North America. Interactions between the biological control strain and the NA strain could include competitive exclusion, co-infection within hosts or hybridisation. We reared D. siricidicola Kamona on an A. areolatum strain native to North America (IGS-BE) and another strain (IGS-BDF) used commercially to mass-produce the nematode in Australia. We inoculated Kamona reared on either strain of A. areolatum into logs infested with S. noctilio larvae and compared parasitism rates against control logs. Individual nematodes were isolated from S. noctilio hemocoels and from sterilised eggs and were genotyped with eight microsatellite loci. A high rate of parasitisation of S. noctilio by D. siricidicola NA was found for all treatments and we found evidence of both co-infection and hybridisation. Surprisingly, sterilisation rates were not related to the rates of parasitisation by D. siricidicola Kamona.

Published
2019
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2. Sensitive environmental DNA (eDNA) methods to detect hemlock woolly adelgid and its biological control predators Leucotaraxis silver flies and a Laricobius beetle

Author

Anish Kirtane, Nicholas J. Dietschler, Tonya D. Bittner, Marshall Bigler Lefebvre, Sabrina Celis, Katharine O'Connor, Nathan Havill, and Mark C. Whitmore

Subjects
Adelges tsugae, biological control, biotype‐specific qPCR, eDNA, hemlock woolly adelgid, invasive species, Environmental sciences, GE1-350, Microbial ecology, QR100-130
Abstract

Abstract Environmental DNA (eDNA) analysis can be a powerful tool for the early detection of invasive organisms. However, research on terrestrial eDNA detection from foliage surfaces has been limited. In this study, we developed methods to capture and detect eDNA using qPCR from an invasive forest pest, hemlock woolly adelgid (Adelges tsugae), and three of its biological control predators Leucotaraxis piniperda, Leucotaraxis argenticollis, and Laricobius nigrinus. We designed four highly efficient qPCR assays with a low limit of detection (1–10 copies/reaction). The assay targeting A. tsugae was species‐specific. The assays targeting Le. piniperda, and Le. argenticollis were biotype‐specific in addition to being species‐specific demonstrating applications of eDNA analysis beyond species‐level detection. The La. nigrinus assay also detected DNA from closely related and hybridizing Laricobius rubidus. The eDNA methods were evaluated against traditional detection methods. We collected foliage samples from three strata (bottom, middle, and top) of eastern hemlock trees to detect the presence of A. tsugae. The detection of the biological control predators was evaluated using western hemlock foliage samples collected from the predators' native range in western Washington. The eDNA methods had significantly higher positive detection rates (2.8–4.5 times) than conventional methods of all target species. The strata of sampling were not significant in determining the presence of A. tsugae infestation. The eDNA concentration positively correlated with the observed density for all species. This study demonstrates the efficacy of eDNA analysis as a more sensitive tool for early detection of A. tsugae and to track the establishment of its biological control predators.

Published
2022
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3. Permanent Genetic Resources added to Molecular Ecology Resources Database 1 June 2010 - 31 July 2010

Author

Cynthia Riginos, Rasanthi M. Gunasekera, James P. Hereward, Adam E. Vorsino, C. C. Vinson, Philippe Jarne, H. A. Rose, Joël Bried, Kittipath Prapayotin-Riveros, A. M. Risterucci, Angélique Quilichini, Mark G. Wright, Asta Audzijonyte, Hélène Delatte, Rebecca N. Johnson, Ryan A. Thum, Charles R. Vossbrinck, Yan Hong, Chunhong Li, Byeong-Wha Min, Akihiro Kijima, Pierre-Jean G. Malé, P. Charruau, Andrew J. Lowe, Karen-Ann Gray, Gregory J. Bugbee, A. Y. Ciampi, Woo-Jin Kim, Gudbjorg I. Aradottir, Steven J. Hanley, Minghui Liao, Jean-François Martin, Benjamin Fartek, Jae K. Noh, Manami Kanno, Bernard Reynaud, Hyun Chul Kim, K. Silbermayr, Paul J. DeBarro, Thibaut Malausa, D. Petro, Michelle D. Marko, Michael G. Gardner, Ania M. Wieczorek, Qi Jianfei, Chris Walzer, N. Tero, Samuel Nibouche, Kwan S. Kim, Emilie C. Bess, Ann E. Noack, Jeong-Ho Lee, Shotaro Hirase, G. Bourdel, Malvina Andris, Iracilda Sampaio, Mark D. B. Eldridge, Choul-Ji Park, Pamela A. Burger, Jang W. Lee, Nathan Havil, Jason C. White, D. Enkhbileg, Russell H. Messing, Jérôme Orivel, Laurent Costet, Jeong-In Myeong, Karen Miller, G. Arnau, Phillip R. England, Nathan Lo, Francesco Bonadonna, Elizabeth A. LaRue, Marivana Borges Silva, Marie-Pierre Dubois, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Paul-Valéry - Montpellier 3 (UM3), Department of Biomedical Sciences, Institute of Population Genetics, University of Veterinary Medicine, University of Veterinary Medicine, Research Institute of Wildlife Ecology, University of Veterinary Medicine, Department of Epidemiology, Biostatistics and Occupational Health [Montréal], McGill University, Institut de Chimie de Clermont-Ferrand (ICCF), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Sigma CLERMONT (Sigma CLERMONT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Evolution, Génomes et Spéciation (LEGS), Centre National de la Recherche Scientifique (CNRS), School of Physics and Astronomy, University of Birmingham [Birmingham], Division of Cardiovascular Medicine, University of Cambridge [UK] (CAM), Birkbeck College [University of London], Department of Mathematical Sciences, TJ Watson, IBM, Southeast University [Jiangsu], State Key Lab. of Information Engineering in Surveying, Mapping and Remote Sensing, Wuhan University [China], Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Evolution et Diversité Biologique (EDB), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Ecologie des forêts de Guyane (ECOFOG), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université des Antilles et de la Guyane (UAG)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), School of Mechanical and Chemical Engineering, The University of Western Australia (UWA), Institut de recherche agricole pour le développement (IRAD), Ministère de la Recherche et de l'innovation, Department of Physics and Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Paris-Jourdan Sciences Economiques (PSE), Centre National de la Recherche Scientifique (CNRS)-École des Ponts ParisTech (ENPC)-École des hautes études en sciences sociales (EHESS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Paris (ENS Paris), Polymorphismes d'intérêt agronomique (UMR PIA), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Technologie Servier, Institute of Arctic and Alpine Research (INSTAAR), University of Colorado [Boulder], Centre de recherche sur l'espace sonore et l'environnement urbain [1998-2014] (CRESSON [1998-2014]), Ambiances architecturales et urbaines (AAU), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Nantes (ECN)-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Nantes (ECN)-Ministère de la Culture et de la Communication (MCC), Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), McGill University = Université McGill [Montréal, Canada], Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-SIGMA Clermont (SIGMA Clermont)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Sophia Agrobiotech (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Recherche Agronomique (INRA)-École des hautes études en sciences sociales (EHESS)-École des Ponts ParisTech (ENPC)-Centre National de la Recherche Scientifique (CNRS), Institute of Arctic Alpine Research [University of Colorado Boulder] (INSTAAR), Centre de recherche sur l'espace sonore et l'environnement urbain (CRESSON), École Centrale de Nantes (ECN)-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Nantes (ECN)-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, École normale supérieure - Paris (ENS Paris), and Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture et de la Communication (MCC)-École Centrale de Nantes (ECN)-Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture et de la Communication (MCC)-École Centrale de Nantes (ECN)

Subjects
0106 biological sciences, Identification, [SDV]Life Sciences [q-bio], Banque de gènes, computer.software_genre, 01 natural sciences, F30 - Génétique et amélioration des plantes, GENBANK, Marqueur génétique, biology, Database, CATALOGUE, Dioscorea rotundata, Dioscorea trifida, Dioscorea, GENETIQUE DES POPULATIONS, Banque de données, ECOLOGIE, Biotechnology, Écologie, PHYLOGENY, Zoology, MOLECULAR MARKERS, Licania, ECOLOGY, 010603 evolutionary biology, Dioscorea transversa, Ressource génétique végétale, REFERENCEMENT, POPULATION GENETICS, Genetics, Ressource génétique animale, Dioscorea pentaphylla, Ecology, Evolution, Behavior and Systematics, Ressource génétique, Biologie moléculaire, TAXONOMY, biology.organism_classification, L10 - Génétique et amélioration des animaux, Tuberolachnus salignus, Hirtella, computer, 010606 plant biology & botany
Abstract

Correspondance: Molecular Ecology Resources Primer Development Consortium, E-mail: editorial.office@molecol.com; International audience; This article documents the addition of 512 microsatellite marker loci and nine pairs of Single Nucleotide Polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Alcippe morrisonia morrisonia, Bashania fangiana, Bashania fargesii, Chaetodon vagabundus, Colletes floralis, Coluber constrictor flaviventris, Coptotermes gestroi, Crotophaga major, Cyprinella lutrensis, Danaus plexippus, Fagus grandifolia, Falco tinnunculus, Fletcherimyia fletcheri, Hydrilla verticillata, Laterallus jamaicensis coturniculus, Leavenworthia alabamica, Marmosops incanus, Miichthys miiuy, Nasua nasua, Noturus exilis, Odontesthes bonariensis, Quadrula fragosa, Pinctada maxima, Pseudaletia separata, Pseudoperonospora cubensis, Podocarpus elatus, Portunus trituberculatus, Rhagoletis cerasi, Rhinella schneideri, Sarracenia alata, Skeletonema marinoi, Sminthurus viridis, Syngnathus abaster, Uroteuthis (Photololigo) chinensis, Verticillium dahliae, Wasmannia auropunctata, and Zygochlamys patagonica. These loci were cross-tested on the following species: Chaetodon baronessa, Falco columbarius, Falco eleonorae, Falco naumanni, Falco peregrinus, Falco subbuteo, Didelphis aurita, Gracilinanus microtarsus, Marmosops paulensis, Monodelphis Americana, Odontesthes hatcheri, Podocarpus grayi, Podocarpus lawrencei, Podocarpus smithii, Portunus pelagicus, Syngnathus acus, Syngnathus typhle,Uroteuthis (Photololigo) edulis, Uroteuthis (Photololigo) duvauceli and Verticillium albo-atrum. This article also documents the addition of nine sequencing primer pairs and sixteen allele specific primers or probes for Oncorhynchus mykiss and Oncorhynchus tshawytscha; these primers and assays were cross-tested in both species.

Published
2011
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