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This study aimed to evaluate the perception of Brazilian dairy processors (n = 31) concerning food defense. The results showed that respondents consider the implementation of control procedures related to facilities, products, materials, and individuals as important measures in food defense. The higher agreement rates (strongly agreed + slightly agreed) of the companies in relation to the perception of food defense were 84% for external security, followed by personnel security (82%), generalities (81%), and internal security (74%). Thus, protecting facilities and controlling the traffic flow were considered to be the most important actions under the participants' perspectives. Employee satisfaction and identification of end products and raw materials are also considered relevant in the food defense program. Although food defense is not a formal requirement in Brazilian law, the results show that there is adequate awareness of this topic by the Brazilian dairy companies.

Background Thermal processing is the most common decontamination method used in the dairy industry to ensure food safety and extend shelf life. However, due to the increased consumer demand for more natural and healthier products, nonthermal technologies have been intensively studied. Irradiation with shortwave ultraviolet light (UV-C) offers some technological advantages due to its low maintenance and installation costs, minimal energy use, and food preservation without some undesirable effects of heat treatments. Scope and approach: This review aims to describe the theoretical fundamentals of UV-C radiation and approaching the critical processing factors involved. Also, it describes the mechanisms of microbial inactivation and provides an overview of the effects on dairy product quality, considering microbiological, physicochemical and sensory aspects. Key findings and conclusions UV-C radiation can be considered an effective method for inactivating pathogenic and spoilage microorganisms in milk and dairy products by forming lesions in DNA and/or damage in the cellular enzyme activity and cytoplasmatic membrane integrity. The efficiency of the process is dependent on the process parameters (time of exposure, UV dose, wavelength and UV light source), product type (chemical composition, viscosity, turbidity, opacity and roughness), equipment (conformation and geometry), and microorganism characteristics (specie, strain, initial count, growth phase, and recovery conditions). When optimum conditions are applied, there is no impact on the physicochemical, nutritional and sensory aspects. In conclusion, UV-C can be considered an environmental-friendly emergent non-thermal technology for decontamination of dairy products, presenting low costs and efficiency in the maintenance of the quality parameters of dairy products.

Background Postprandial glycemia after food intake is considered the most important parameter to control the risk of diabetes. Although probiotic dairy products may have a regulatory effect on postprandial blood glucose, this topic has been poorly addressed in scientific studies. Scope and approach This review aims to discuss the relationship between the consumption of probiotic dairy products and the maintenance of postprandial glycemia, presenting the mechanisms of action of probiotics and the impact of the dairy matrix, the probiotic strain, and addition of ingredients, among others. A comparison between the effects of dairy products and the synthetic drugs conventionally used was also carried out. Finally, the review deals with strategies for increasing the functionality of probiotic dairy products. Key findings and conclusion Ripened cheeses, fermented dairy products, and whey-based products (milk beverages) containing probiotic strains with higher proteolytic and EPS-forming capacities, and the addition of prebiotics and/or plant-derived products have a greater effect on postprandial glycemic regulation and/or inhibition of α-glucosidase and α-amylase enzymes. The literature has focused on the in vitro inhibitory effect on digestive enzymes, suggesting that clinical studies should be a priority. The inhibitory effect observed for the probiotic dairy products can be quantitatively comparable to that provided by synthetic drugs, depending on the dairy matrix and the probiotic strain, with promising results.

This study aimed to evaluate the effect of the process time (5, 10, and 15 min) and flow rate (10, 20, and 30 mL/min) of cold plasma technology on physio-chemical characteristics (pH), bioactive compounds (DPPD, Total Phenolic Compounds, ACE-inhibitory activity values), fatty acid composition, and volatile compounds profile of chocolate milk drink. The mild (lower flow rate and process time) and more severe (higher flow rate and process time) conditions led to a reduction of the bioactive compounds (total phenolic compounds and ACE-inhibitory activity), changes in fatty acid composition (increased saturated fatty acid and decreased monounsaturated fatty acid and polyunsaturated fatty acid), less favorable health indices (higher atherogenic, thrombogenic and hypercholesterolemic saturated fatty acids and lower desired fatty acids), and lower number of volatile compounds. In contrast, in intermediate cold plasma conditions, an adequate concentration of bioactive compounds, fatty acid composition, and health indices, and increased number of volatile compounds (ketones, esters, and lactones) were observed. Overall, cold plasma technology has proven to be an interesting alternative to chocolate milk drinks, being of paramount importance the study of the cold plasma process parameters.

The present study aimed to evaluate the effect of cold plasma processing parameters (time and flow rate) on the physical (rheological parameters and particle size), microstructure (optical microscopy) and thermal properties (differential scanning calorimetry) of chocolate milk drinks. The beverages treated by cold plasma presented particles with increased size and had higher consistency and altered melting profile (lower temperature and bound water and higher enthalpy) than the pasteurized product, suggesting denaturation processes and formation of protein aggregates. More severe conditions resulted in beverages with particles of larger surface areas ([D 3,2]) and volume diameters ([D4,3]), resulting in higher consistency. Intermediate processing conditions resulted in products with characteristics more similar to the pasteurized beverages. The results indicate that the beverages submitted to cold plasma and pasteurization have different physical characteristics, melting profile and microstructure. Chocolate milk drinks with different characteristics could be obtained varying the cold plasma process parameters.

Background Thermal pasteurization and sterilization are predominantly used in the dairy industry due to their efficacy in improving the product safety and shelf life. However, heat treatment can cause undesirable protein denaturation, non-enzymatic browning, loss of vitamins and volatile flavor compounds, freezing point depression, and flavour changes. Cold plasma is a non-thermal technology that has gained attention in recent years as a potential alternative method for chemical and thermal disinfection in foods using ambient or moderate temperatures and short treatment times. Scope and approach This review aims to describe the fundamentals, parameters, and technology on cold plasma, discussing the critical processing factors involved in this technology. Also, it describes the mechanisms of microbial inactivation and provides an overview of the effects of non-thermal plasma on the quality of dairy products, considering a physicochemical, sensory and microbiology perspective. Key findings and conclusions Cold plasma uses less aggressive mechanisms of action to the milk matrix when compared to the techniques currently used, and has shown an excellent performance on the elimination of pathogenic and spoilage microorganisms besides maintaining, in many cases, the nutritional, functional, and sensory characteristics of the product.

The aim of this study was to determine the total lipid content in nine different body regions of jau catfish ( Zungaro jahu ), and evaluate the nutritional composition, energy value and lipid oxidation of jau meat during 15 days at 4°C, before (T1) and after freezing (T2) for 105 days at –20°C. The body regions were divided into anterior-dorsal (P1), anterior-medial (P2), anterior-ventral (P3), central-dorsal (P4), central-medial (P5), central-ventral (P6), posterior-dorsal (P7), posterior-medial (P8), and posterior-ventral (P9). Jau catfish contained high protein (20.17%), low lipid (0.60%) and low energy value (88.36 kcal 100g -1 ). The increase of malondialdehyde - MDA (0.2 to 2.5 mg MDA/kg) was more pronounced (P

The consumption of probiotic-enriched dairy products has been associated with many health benefits, including anti-hyperglycemic activity. The effect on health is dependent on the type of probiotic culture used and the dairy product consumed. This study evaluated the effect of different probiotic-enriched dairy matrices (Minas Frescal cheese, Prato cheese, and whey dairy beverage) containing Lactobacillus casei on in vitro and in vivo anti-hyperglycemic activity. For this purpose, in vitro anti-hyperglycemic activity was determined by the inhibition of α-glucosidase and α-amylase activities, and a human study was performed with healthy individuals (n = 15, consumption of bread as a control; bread + Minas Frescal cheese; bread + Prato cheese; bread + dairy beverage) to assess the effects of different probiotic foods on postprandial glycemia. In vitro data showed that Prato cheese presented the highest lipid (36.9 g/100 g) and protein (26.5 g/100 g) contents as well as the highest α-amylase (60.7%) and α-glucosidase (52.6%) inhibition. The consumption of Prato cheese resulted in a lesser increase in blood glucose level (13 mg/dL) compared with the consumption of bread alone (19 mg/dL), Minas Frescal cheese (20 mg/dL), and whey dairy beverage (30 mg/dL), with glycemic indices similar to that observed for the control. The present results demonstrated a good correlation between in vitro and in vivo data, in which the type of dairy matrix affects the anti-hyperglycemic activity. It is concluded that the consumption of probiotic Prato cheese can contribute to the reduction of postprandial glycemia in healthy individuals.

The nutritional quality and lipid stability of Arapaima gigas fillets were investigated. A total of 27.32 kg of A. gigas fillets were obtained and the proximate composition and fatty acid profile immediately determined. In addition, the lipid oxidation parameters were analyzed during 15 and 90 days at 4 °C and –20 °C, respectively. The A. gigas fillets presented high protein (> 15%) and low lipid (< 2%) contents with elevated polyunsaturated fatty acid (PUFAs) contents (43.97%). The nutritional quality indices were the atherogenicity index (0.35), thrombogenicity index (0.28) and the hypocholesterolemic acid/ hypercholesterolemic acid ratio (2.37). Overall, an increase followed by a decrease was observed in the peroxide index (PI) and malondialdehyde content (MDA) results at both storage temperatures ( p < 0.05). The lipid profile exhibited great nutritional quality, however new conservation methods should be investigated for this matrix due to increased lipid oxidation during refrigerated and frozen storage. Resumo O objetivo deste estudo foi investigar a qualidade nutricional bem como a estabilidade lipídica de filés de Arapaima gigas. Um total de 27,32 kg de filés de A. gigas foi obtido e a composição centesimal e o perfil de ácidos graxos foram imediatamente determinados. Além disso, os parâmetros de oxidação lipídica foram analisados durante 15 e 90 dias, a 4 °C e -20 °C, respectivamente. Os filés de A. gigas apresentaram elevado teor de proteína (>15%) e baixa quantidade de lipídeos (

This study aimed to evaluate the impact of cold plasma (0, 5, 10, or 15 min) on the techno-functional and sensory properties of whey dairy beverages added with xylooligosaccharide (XOS, 1.5% p/v). Untreated and pasteurized whey beverages were also evaluated. The products were evaluated for physicochemical characteristics, bioactive compounds, XOS stability, rheological properties, and sensory characteristics. Cold plasma and pasteurized products presented lower color intensity (L*=87.4–87.9, a*=-0.24- −0.60, b*=2.41–5.19), reduced consistency (K = 4.31–42.21 mPa.sn and N = 0.57–0.95), and similar apparent viscosity, XOS chemical stability, and sensory characteristics compared with the untreated product. However, the cold plasma-treated beverages presented lower heat load indicators (hydroxymethylfurfural [HMF] values of 1.91–2.10 µmol/L and whey protein nitrogen index [WPNI] of 6.09–6.66 µmol/L) and a higher concentration of bioactive compounds (antioxidant activity [5.31–9.30%], and inhibition of ACE [14.17–22.53%], α-amylase [18.52–25.67%] and α-glucosidase [22.50–27.50%] activities) than the pasteurized product, being the effects more pronounced for the higher exposure times. Overall, cold plasma has important advantages for the processing of whey beverages added with XOS.

This study aimed to evaluate the consumers' perception (n = 1085) about processing chocolate milk drinks by cold plasma. The consumers were asked about food technology neophobia, familiarity and, willingness to buy the product, sensory attributes, and perceived quality compared to traditional technologies. The consumers considered that new technologies could have a negative impact on health, natural quality of the product, and environment, that the benefits of the new technologies are overstated, and that could be a risk replacing the traditional technologies. However, most of the consumers (72.3%) were willing to buy the chocolate milk drink processed by cold plasma, mainly if the price is similar to that of the conventional product. Chocolate milk drink should have a brown color, chocolate flavor, typical chocolate and milk taste, and high consistency. The willingness to buy the new product increases with the belief that the new technologies would not bring negative health effects, while it decreases with the unfamiliarity with new technologies, doubts about the information provided by the media, or no perceived quality compared to the traditional technology. Therefore, it is important that the food industry provide consumers with trustable, understandable, and clear information so that the lack of confidence could be overcome.

This study aimed to evaluate the effect of addition of xylooligosaccharide (XOS) and galactooligosaccharide (GOS) to whey protein (WP) films produced by casting technique and using glycerol as a plasticizer. The films were evaluated for thickness, scanning electron microscopy (SEM), differential scanning calorimetry (DSC), mechanical properties, water vapor permeability (WVP) and contact angle. No effect of the addition of prebiotic components on thickness of the films was observed (0.13–0.14 mm, p > 0.05). XOS and GOS addition resulted in similar cross-sectional SEM images, presenting smaller and retracted laminated structures, with lower tensile strength and higher elongation at break (3.8–1.7 MPa and 144–208%, respectively, p

The perception of Brazilian non-bovine dairy processors (goat, sheep and buffalo, n = 32) located in eight states of Brazil (Rio de Janeiro, Sao Paulo, Minas Gerais, Parana, Goias, Bahia, Rio Grande do Sul, and Ceara), regarding the implementation of traceability was investigated. A questionnaire consisting of 16 statements using a 5-point Likert Scale was applied and the data were evaluated by descriptive statistics and factor analysis. It was agreed that the implementation of a traceability system allows a quick recall of products, and reduces the negative impact, the number of consumer complaints and the loss of products. In addition, it can result in increased supplier control and process safety, protecting consumer's health and increasing their confidence. However, the relationship between the traceability system and the cost savings in the process and/or price of the final product was not a consensus. Although dairy companies agreed that traceability is highly relevant to the company, many are unwilling to invest in implementation of the system. Therefore, non-bovine milk-producing units in Brazil are aware of the impact of implementing traceability, but still have doubts about the costs involved, which restrict investments in the system.

The effect of cold plasma processing time and gas flow on bioactive compounds such as vitamin C, carotenoids and phenolic compounds, DPPH, angiotensin-converting-enzyme (ACE) inhibitory activity, fatty acids profile, and volatile compounds of guava-flavored whey beverage was investigated. For comparative purposes, a pasteurized beverage was also manufactured. Cold plasma increased the concentration of bioactive and volatile compounds, and proportionated changes in the fatty acids profile. The milder conditions like lower flow rate and processing time, resulted in higher vitamin C and volatile compounds levels, and higher antioxidant activity, but with a lower carotenoids content and a less favorable fatty acids profile. More drastic conditions like higher flow rate and processing time resulted in products with lower vitamin C and volatile compounds levels, but with higher carotenoids content and ACE inhibitory activity. It can be concluded that the cold plasma processing can improve the properties of the guava-flavored whey beverages (increased concentration of bioactive and volatile compounds), while the effect on the fatty acid profile and ACE inhibitory activity is dependent on the process parameters (processing time and flow rate).

This study aimed to evaluate the efficacy of time-domain nuclear magnetic resonance (TD-NMR) to determine the formaldehyde in milk samples (from 1.75 to 21% v/v) using chemometric methods (principal component analysis, partial least squares regression, and soft independent modeling of class analogy, PCA, PLS, and SIMCA), when compared with the official colorimetric method. The analysis was performed at different times of refrigerated storage (0 h and 48 h). Increased formaldehyde concentration (17.5 and 21%) led to a decrease in the whiteness index [65.3–72.8] and the lightness values [70.5–85.3] and an increase in the yellowing index [36.31–38.73] compared to the control [77.8–78.7, 85.2–90.3, and 27.40–29.92, respectively]. Regarding the TD-NMR values, higher T2 values were observed, with the formation of T2,1 and T2,2 throughout the refrigerated storage. PCA and PLS proportionated adequate models while SIMCA was able to discriminate the samples regards the level of adulteration with adequate discrimination and predictive index. Overall, TD-NMR has proven to be an effective method for the dairy industry to check the formaldehyde levels in raw milk.

The present study aimed to compare the physicochemical (pH), physical (rheology parameters and particle size), microstructure (optical microscopy) and thermal properties (differential scanning calorimetry) of guava flavored whey-beverages submitted to cold plama technology in different processing time (5, 10, and 15 min) and gas flow (10, 20, and 30 mL min−1) conditions with a conventional pasteurized product. Whey beverages treated by cold plasma presented higher pH values, lower consistency and lower viscosity, and a flow behavior index similar to Newtonian fluids. Milder cold plasma conditions resulted in whey beverages with higher pH, lower viscosity and consistency, and similar particle distribution and microstructure compared to the pasteurized product. In contrast, more severe processing conditions resulted in a higher particle surface area ([D 3,2]) and smaller particles (~10 μM), due to the decrease in the number of larger particles (1000 μM), cell rupture, the formation of cell fragments, and higher viscosity and consistency. The treatments did not affect the thermal properties (enthalpy and bound water) of any sample.