1. Death effects of reveromycin A in normal and disease‐associated cells of the joint
- Author
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Calli Williams, Nathan Granger, Natassja Thomas, Austin Greer, Jaylin Miller, Daniel Jones, Ryan Sabo, Mark A. Lipton, Haley Svrcina, Ellen Steinke, Seth Baker, Patricia Martinez, Lauren Nugent, Morgan Smith, Kelsey Davitt, and Emily Sloane
- Subjects
0301 basic medicine ,Fibroblast-like synoviocyte ,Necrosis ,Mitomycin ,Apoptosis ,Caspase 8 ,Biochemistry ,Chondrocyte ,Cell Line ,Arthritis, Rheumatoid ,Mice ,03 medical and health sciences ,Western blot ,medicine ,Animals ,Humans ,Spiro Compounds ,Molecular Biology ,Pyrans ,biology ,medicine.diagnostic_test ,Cytochrome c ,Synovial Membrane ,Mitomycin C ,Cell Biology ,Fibroblasts ,Hydrogen-Ion Concentration ,Molecular biology ,030104 developmental biology ,medicine.anatomical_structure ,Immunology ,biology.protein ,medicine.symptom - Abstract
Earlier work in our laboratory demonstrated that naturally occurring reveromycin A (Rev A) causes apoptosis in osteoclasts without accompanying necrosis. Rev A death effects in both normal and diseased joint cells were investigated in this study. 10 □M Rev A did not cause apoptosis nor necrosis in monolayer chondrocytes, even at pH 6.8, a pH mimicking that of an inflamed joint. In contrast, at the acidic pH Rev A did induce significant apoptosis (four-fold increase at 48 hours of treatment, p
- Published
- 2018
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