14 results on '"Natalia Nevskaya"'
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2. Crystal Structure of the 23S rRNA Fragment Specific to r-Protein L1 and Designed Model of the Ribosomal L1 Stalk from Haloarcula marismortui
- Author
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Azat Gabdulkhakov, Svetlana Tishchenko, Alisa Mikhaylina, Maria Garber, Natalia Nevskaya, and Stanislav Nikonov
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Haloarcula marismortui ,ribosomes ,archaea ,23S rRNA ,X-ray crystallography ,Crystallography ,QD901-999 - Abstract
The crystal structure of the 92-nucleotide L1-specific fragment of 23S rRNA from Haloarcula marismortui (Hma) has been determined at 3.3 Å resolution. Similar to the corresponding bacterial rRNA fragments, this structure contains joined helix 76-77 topped by an approximately globular structure formed by the residual part of the L1 stalk rRNA. The position of HmaL1 relative to the rRNA was found by its docking to the rRNA fragment using the L1-rRNA complex from Thermus thermophilus as a guide model. In spite of the anomalous negative charge of the halophilic archaeal protein, the conformation of the HmaL1-rRNA interface appeared to be very close to that observed in all known L1-rRNA complexes. The designed structure of the L1 stalk was incorporated into the H. marismortui 50S ribosomal subunit. Comparison of relative positions of L1 stalks in 50S subunits from H. marismortui and T. thermophilus made it possible to reveal the site of inflection of rRNA during the ribosome function.
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- 2017
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3. Weakening of granitoid gouge in hydrothermal ring shear experiments
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Weijia Zhan, Natalia Nevskaya, André Niemeijer, Alfons Berger, Chris Spiers, and Marco Herwegh
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Fault gouges of granitoid composition represent the principal non-cohesive tectonites within fault zones in the continental crust. The spatial distribution and strength of granitoid fault gouges is therefore crucial for understanding how weak the upper continental crust could be due to the formation of fault zones. Although several laboratory investigations reported the mechanical weakening of granitoid gouges in shear experiments, the deformation mechanism responsible for such behavior remains not well understood.To address this issue, we conducted two series of shear experiments on granitoid gouges by using a ring shear apparatus. The starting gouge powders were derived from crushed granitoid mylonite with a median grain size of 45 μm. In a first set of experiments, gouges were sheared at a sliding velocity of 100 μm/s for a displacement of 15 mm. Temperatures explored ranged from 20°C to 650°C in order to determine the temperature dependence of gouge strength. The second set of experiments is identical to the first ones, except that the applied sliding velocity was set at 1 μm/s to study how fault slip rate influences the strength of gouges.We observe that differences in gouges strengths as a function of sliding velocity and temperature: At a sliding velocity of 100 μm/s, the steady-state shear stress (τ) remains relatively constant at τ=76-82 MPa over the entire temperature range. Contrastingly, at a sliding velocity of 1 μm/s the steady-state shear stress remains temperature-insensitive with τ≈75 MPa up to tempertures of 450°C, but decreases then to τ≈50 MPa at 650°C (Fig.1 a). Furthermore, the amount of decrease of shear stress is strain dependent (Fig.1 b). At even slower sliding velocity of 0.1 µm/s, the shear stresses decrease further to τ≈38 MPa.Microstructurally, all gouges deformed at T≦450°C show typical cataclastic features, where angular clasts with grain size of ~10 μm are surrounded by a fine-grained matrix. Intergranular fracture arrays in Riedel- and Y-shears are well developed over the entire cross section, indicating homogeneous bulk deformation. In contrast, gouges sheared at 650°C with τ≈50 MPa show strain localization in a principal slip zone. It is shear plane parallel with widths up to ~50 µm. Inside the principal slip zone, all grains are dramaticly reduced to nm-size and tightly packed. No intergranular fracture arrays are observed. Outside the principal slip zone, rounded grains with size of ~5 μm are loosely packed, with meniscus cement growing in between. The aforementioned strain localization is enhanced at temperature above 450°C and slip rate below 1μm/s, suggesting that viscous creep mechanisms (e.g. pressure solution) control the deformation process at slow sliding velocities, which is not the case in fast rate experiments. Our results show that the activation of viscous creep mechanisms leads to significant fault zone weakening, while contrasts in grain size keep deformation localized.Figure 1 Shear stress plotted as a function of temperature. Shear stress data collected at (a) 15mm displacement in steady-state, and at (b) 5mm displacement.
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- 2023
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4. Experimental evidence for viscous deformation and strain localization in fractured granitoid rocks
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Natalia Nevskaya, Weijia Zhan, Holger Stünitz, Alfons Berger, and Marco Herwegh
- Abstract
According to well-established hypotheses based on field observations of natural faults, viscous deformation may localize following pre-existing brittle fractures. The weak behaviour can be explained by brittle grain size reduction and phase mixing, which may activate grain size sensitive processes in the viscous field. To prove this hypothesis, it is necessary to perform experiments to observe the strain and stress evolution in faulted and non-faulted rocks. Pec et al. (2012) performed experiments on granitic rocks by shearing manually crushed granitic powder between coarse solid granitic forcing blocks. However, in their study, there are unavoidable boundary conditions between the forcing blocks and the gouge, and a comparison to an intact rock without fracture is difficult.In our study, we reduce the boundary conditions to a minimum and can directly compare the stresses and microstructural evolution during deformation of intact and fractured granitic ultramylonites at 650°C, confining pressure of 1.2GPa, and a constant displacement rate of 10-8m/s. We perform these experiments on initially solid cylindrical samples in two experimental sets: In set A, we slowly apply the load and confining pressure, to ensure an intact rock sample is deformed. In set B, we create fractures before the experiment starts but already in the closed system of the experimental setup. Once experimental P/T conditions are reached, both experimental sets are deformed to different finite strains to investigate the associated microstructural evolution. The deformation is disseminated in the set A experiments, but localizes strongly along the fracture in experimental set B. The strain is accommodated by viscous granular flow incorporating an impressive grain size reduction of up to 1000x and dissolution/precipitation processes. In addition, the stress records show that in experiments A, initially a 30% higher yield stress has to be overcome before steady state flow, while in set B steady state flow is reached directly without a strain softening increment. In both sets, steady state stresses range around 300MPa, i.e. far below the confining pressure.Applying microstructural observations and mechanical data of our experiments to deformation of granitoid crust in nature reveals that fractures serve to reach mechanical steady state earlier compared to non-fractured crust. As a matter of strain, however, both settings may yield at the same mechanical strengths of resulting shear zones. It is important to note that polymineralic fine-grained ultramylonites are up to four times weaker than monomineralic quartz, presenting an important behaviour of efficient strain localization and rheological properties substantially below those of the end member minerals. Pec, M., Stünitz, H. and Heilbronner, R., 2012. Semi-brittle deformation of granitoid gouges in shear experiments at elevated pressures and temperatures. Journal of Structural Geology, vol. 38, pp. 200-221. https://doi.org/10.1016/j.jsg.2011.09.
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- 2023
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5. The Digitalization of Russia-EU trade
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Natalia Nevskaya
- Subjects
Economics and Econometrics ,Political Science and International Relations ,Business - Published
- 2020
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6. Velocity-dependent friction of granitoid gouge under hydrothermal conditions: A contribution to understanding of fault zone seismicity
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Weijia Zhan, André Niemeijer, Natalia Nevskaya, Alfons Berger, Chris Spiers, and Marco Herwegh
- Abstract
Fault gouges of granitoid composition represent the principal non-cohesive tectonites within fault zones in the continental crust. Their velocity-dependent friction is crucial for understanding earthquake nucleation and the depth distribution of fault-related seismicity in granitoid shear zones (Wehrens et al. 2016; Blanpied et al. 1998). In the framework of rate-and-state friction laws (RSF), the friction parameter (a-b) is measured in sliding experiments to describe the velocity dependence of friction in fault gouges (Scholz, 1998). A velocity-strengthening system is frictionally stable, (a-b) >0, whereas a velocity-weakening system can be frictionally unstable, (a-b) To address this issue, we conducted velocity stepping sliding experiments under hydrothermal conditions by using a ring shear apparatus. The powdered starting gouge was derived from a granitoid mylonite collected at the NAGRA Grimsel Test Site (Central Swiss Alps). The applied velocity steps were 1-3-10-30-100 μm/s. Pore fluid pressure and the effective normal stress were 100 MPa. Temperatures explored ranged from 20-650 °C. Values of (a-b) were obtained from RSF model inversions of the evolution of friction coefficients at mechanical steady state conditions. Our experiments showed pronounced changes in (a-b) values with across the full range of temperatures up to 650 °C and velocities investigated. At temperatures below ~100 °C and above ~400 °C, we observed mostly velocity strengthening with positive (a-b). In contrast, velocity weakening with negative (a-b) was observed between ~100 °C and ~400 °C. Samples deformed at a sliding velocity of 100 μm/s deviated slightly from this trend, as (a-b) values were negative between ~200 °C and ~400 °C.The presented experimental study demonstrates a significant influence of temperature and sliding velocity on velocity-dependence during deformation of granitoid gouge. We suggest that the observed transitions in velocity dependence reflect an interplay of interactions. In terms of crustal faulting, our data suggest the existence of a seismogenic window that limits the depth distribution of earthquakes on faults in granitoid shear. REFERENCESWehrens, P. C., Berger, A., Peters, M., Spillmann, T., Herwegh, M. 2016: Deformation at the frictional-viscous transition: Evidence for cycles of fluid-assisted embrittlement and ductile deformation in the granitoid crust, Tectonophysics, 693, 66-84.Blanpied M. L., Tullis T. E., Weeks J. D. 1998: Effects of slip, slip rate, and shear heating on the friction of granite.Scholz, C. H. 1998: Earthquakes and friction laws, Nature, 391, 37-42.
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- 2022
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7. Experimental strain localization in granitoid ultramylonites: Pre-fracturing vs. viscous strain localization
- Author
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Natalia Nevskaya, Weijia Zhan, Holger Stünitz, Alfons Berger, and Marco Herwegh
- Abstract
Rheological models of Earth’s granitoid mid- to upper crust are commonly based on the physico-chemical properties of the most abundant rock forming minerals quartz and feldspar. However, there is increasing field evidence that deformation in these rocks localizes in ultrafine-grained polymineralic shear zones, which are weaker than any of the end member minerals. Especially at the brittle to viscous transition, the localization and deformation mechanisms, i.e. the role of incipient brittle deformation vs. continuous viscous strain localization, is not yet fully understood.To fill this gap in knowledge, ultramylonite samples with granitic composition from the Central Aar Granite (Aar Massif, Central Switzerland) were deformed using a Griggs type apparatus. The foliation of the ultramylonitic starting material was oriented 45° to the compression direction, to investigate the influence of grain size and composition on strain localization in the different mylonite bands. Two types of coaxial experiments were conducted at 650°C, and 1.2 GPa confining pressure: A) Discrete fractures were created before the shear deformation starts; B) No fractures were induced during an early stage of the experiment.All experiments have in common that strain is accommodated in 20-100 µm wide viscous shear zones with elongated grains and minor grain size reduction. In these shear zones, most strain is further localized in 10-20 µm wide zones, showing dramatic grain size reduction down to few tens of nanometres. In the experimentally generated shear zones, both, brittle and viscous processes are active. In terms of overall rock strength, all newly formed ultrafine-grained shear zones are up to three times weaker than comparable experiments on pure quartz or coarser grained granites – which agrees well with field observations. Furthermore, pre-fractured type A) is up to two times weaker than the non-fractured type B), and the orientation and number of shear zones is also fundamentally different between the two experiment types.This study confirms two weakening factors promoting different types of strain localization at the brittle to viscous transition: 1) The existence of fractures and their interconnectivity – facilitating highly-localized grain size reduction; 2) Initial sample heterogeneity by polymineralic composition and ultrafine grain size – generating grain size reduction along strain gradients by activating viscous processes. Further quantitative microstructural analyses will reveal the role of chemistry and the deformation mechanisms on the localization behaviour.
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- 2022
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8. Northern Sea Route and the New Energy Agenda
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Natalia Nevskaya
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- 2022
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9. European Investments Opportunities After COVID-19
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Natalia Nevskaya
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- 2021
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10. Post-COVID Economic Revival: Financial Aspects of Reform
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Natalia Nevskaya
- Published
- 2021
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11. Post-COVID Economic Revival, Volume I
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Natalia Nevskaya
- Published
- 2021
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12. Денежно-кредитное регулирование в России и ЕС
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Vladimir Olenchenko, Anatoliy Bazhan, Natalia Nevskaya, Viktor Iokhin, Viktor Pischik, Alexey Kuznetsov, Kirill Gusev, Victoria Antyushina, and Valentin Fedorov
- Abstract
В брошюре предпринят сравнительный анализ денежно-кредитной политики ЕЦБ и Центрального банка РФ с целью выявить недостатки, устранение которых могло бы способствовать совершенствованию системы регулирования денежного оборота в нашей стране. Авторы работы считают, что одним из главных таких недостатков служит однобокая ориентация российской монетарной политики исключительно на борьбу с инфляцией, что определяет низкий уровень монетизации национальной экономики и тормозит экономический рост. Показано, что в отличие от России, в еврозоне и во многих других странах эта политика направлена на сочетание усилий по стимулированию экономического развития и ограничению инфляционных тенденций. Значительное место в работе отведено исследованию участия центральных банков РФ и ЕС в цифровизации сферы денежного оборота. Несмотря на некоторые различия в позициях, авторы приходят к единому выводу, что осторожное отношение обоих регуляторов к использованию криптовалют основано на правильном понимании природы последних и их роли в современной экономике.
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- 2020
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13. Crypto-assets status in the monetary policy of ECB and bank of RUSSIA
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Natalia Nevskaya
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Computer science ,Monetary policy ,General Medicine ,Monetary economics - Published
- 2019
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14. Domain I of ribosomal protein L1 is sufficient for specific RNA binding
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Natalia Davydova, Natalia Nevskaya, Maria Garber, Vladislav Kljashtorny, Svetlana Tishchenko, Stanislav Nikonov, Olga Kostareva, Victor Streltsov, Ekaterina Nikonova, and Wolfgang Piendl
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Models, Molecular ,Ribosomal Proteins ,EGF-like domain ,Protein domain ,Molecular Sequence Data ,Biology ,Crystallography, X-Ray ,HAMP domain ,03 medical and health sciences ,SeqA protein domain ,Bacterial Proteins ,EVH1 domain ,Genetics ,B3 domain ,Amino Acid Sequence ,RNA, Messenger ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,Binding Sites ,Thermus thermophilus ,030302 biochemistry & molecular biology ,Molecular biology ,Cell biology ,Protein Structure, Tertiary ,RNA, Ribosomal, 23S ,Cyclic nucleotide-binding domain ,Binding domain ,Protein Binding - Abstract
Ribosomal protein L1 has a dual function as a ribosomal protein binding 23S rRNA and as a translational repressor binding its mRNA. L1 is a two-domain protein with N- and C-termini located in domain I. Earlier it was shown that L1 interacts with the same targets on both rRNA and mRNA mainly through domain I. We have suggested that domain I is necessary and sufficient for specific RNA-binding by L1. To test this hypothesis, a truncation mutant of L1 from Thermus thermophilus, representing domain I, was constructed by deletion of the central part of the L1 sequence, which corresponds to domain II. It was shown that the isolated domain I forms stable complexes with specific fragments of both rRNA and mRNA. The crystal structure of the isolated domain I was determined and compared with the structure of this domain within the intact protein L1. This comparison revealed a close similarity of both structures. Our results confirm our suggestion that in protein L1 its domain I alone is sufficient for specific RNA binding, whereas domain II stabilizes the L1-rRNA complex.
- Published
- 2007
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