Search

Gel electrophoresis (GE) is the most preferred and adapted technique for the separation and identification of biological molecules like proteins/peptides and nucleic acids from diverse types of organisms. All over the world, researchers, educators, and students aspiring to work in biochemistry and molecular biology disciplines use the polyacrylamide gel electrophoresis (PAGE) technique for resolving proteins/nucleic acids for understanding the structure and function of any cell. A simple PAGE technique requires a wide range of chemicals/reagents along with a well-equipped and well-spaced laboratory. We have developed a compact and impeccable mobile electrophoresis kit suitable for any vertically oriented PAGE technique. This comprehensive and portable laboratory set-up provides the complete advantages of safety, cost-efficiency, space management, and utility to the researchers for high-throughput research. All new equipment of the mobile electrophoresis kit is fabricated using inexpensive and off-the-rack components. Overall performance of the mobile kit was verified through a practical exercises executed by high school students with positive outcomes.

Recent advancements in biochemical sciences have helped the researchers to explore the molecular logic of life inclusive of its multifarious expressions and explain many facts about the structure and functions of cellular macromolecules. Due to its simple and cost-effective nature, polyacrylamide gel electrophoresis (PAGE) has become the most favored technique for qualitative and quantitative examination of macromolecules. Major drawbacks of such modifications are the cost and operational complexities faced by naïve students. Many interlinking laboratory equipment are needed in the school laboratories for the conduct of even simple scientific experiment. Some of these costly modern equipment are inaccessible for students of small laboratories, and their alternatives are not easily available. Many of these laboratory equipment required for routine gel electrophoresis technique can be fabricated in their simplest form using off-the-shelf components. A short term biochemistry training program was executed for high school students to provide them "hands-on" training using newly modified equipment, which was proved to be an exciting way of learning biochemical gel separation techniques.

Polyacrylamide gel electrophoresis (PAGE) is the most classical technique favored worldwide for resolution of macromolecules in many biochemistry laboratories due to its incessant advanced developments and wide modifications. These ever-growing advancements in the basic laboratory equipments lead to emergence of many expensive, complex, and tricky laboratory equipments. Practical courses of biochemistry at high school or undergraduate levels are often affected by these complications. Two dimensional gel electrophoresis technique (2D-PAGE) used for resolving thousands of proteins in a gel is a combination of isoelectric focusing (first dimension gel electrophoresis technique) and sodium-dodecylsulphate PAGE (second dimension gel electrophoresis technique or SDS-PAGE). Two different laboratory equipments are needed to carry out effective 2D-PAGE technique, which also invites extra burden to the school laboratory. Here, we describe a low cost, time saving and simple gel cassette for protein 2D-PAGE technique that uses easily fabricated components and routine off-the-shelf materials. The performance of the apparatus was verified in a practical exercise by a group of high school students with positive outcomes.

Reproductive biology of female amphidromous croaker Johnius coitor (Hamilton, 1822) was studied for the first time from various freshwater stretches of Ganga river basin, India in relation to climatic variability. The species showed high spatial variation in reproductive phenology and capable of breeding during pre-monsoon, monsoon, post-monsoon and winter. Water temperature is the most crucial environmental parameter influencing gonadal maturation and breeding. Generalized additive model (GAM) models revealed water temperature near 23–25 °C as optimum and threshold GSI above 3 units necessary for breeding. Pre-spawning fitness (Kspawn50) and size at 50% maturity (LM50) benchmarked through Kaplan-Meier survival fit estimates were in the range 1.27–1.37 units and 19–24.5 cm respectively. First maturity of females was encountered at 11.4 cm within the size range 7.2–28.5 cm. Egg parameters in mature-ripe females ranged between 0.29–0.80 mm (diameter), 0.05–0.19 mg (weight) and 5687–121 849 eggs (absolute fecundity). Mapping of climate preferendum through LOESS smoothing technique hinted water temperatures 32 °C to be detrimental for attainment of pre-spawning fitness while no dependence on rainfall was observed. Based on the climato-hydrological influence on breeding and regional trends of changing climate along river Ganga, we infer minimal climate driven changes in breeding phenology of this amphidromous fish species. Results of this study may serve as baseline information for future studies assessing climate driven changes and evolutionary adaptations in croakers from river Ganga.

Floodplain wetlands are among the most threatened ecosystems. It is highly vulnerable to climate change, affecting the wetland ecosystem and its associated fisheries. In the face of data deficiency, this article presents an alternative approach to assess the vulnerability of wetland fisheries to climate change. A total of 15 wetlands from a wetland-fisheries-rich area of West Bengal, India, were selected to illustrate the methodology. The proposed method mainly relies on stakeholders' perceptions of the wetland fisheries vulnerability. Elicitation stakeholders' response iteratively screened the indicators of fisheries vulnerability. The hybrid method of index-based vulnerability comprises two types of indicators: climate indicators with long-term quantitative data and stakeholder perceived vulnerability indicators specific to wetland fisheries. The high level of consensus (92%) among respondents provided strong evidence of climate change in last 15 years, which has also been validated through long-term data analysis. The Principal Component Analysis extracted five synthesized vulnerability indicators, explaining 83.35% variability of the original 14 indicators. The wetlands were further grouped according to differential vulnerability for prioritizing the wetlands for strategic planning. Climate change, reduction in species richness and adaptive capacity were the key components responsible for differential vulnerability. The study also revealed some indigenous climate-smart mitigation strategies of wetland fisheries to climate change. [ABSTRACT FROM AUTHOR]

India is bestowed with rich inland water resources -- viz. ponds, lakes & reservoirs, and rivers among others. Inland fish production -- especially from reservoirs - over the years has grown steadily contributing much to the nutritional security of the people, poverty alleviation, community development and women empowerment. Recent advancements in modeling offer tremendous scope to determine population harvest from open waters. In reservoirs, fish harvest levels are of paramount importance because they should ensure sustainability of the fisheries and economic viability. Since stocking is resorted to enhance fish production in reservoirs these days, in this paper, a modeling approach has been attempted for sustainable fishery exploitation in stocked reservoirs. Accordingly, a modified Verhulst-Schaefer model that takes into account the impact of stocking of fingerlings in reservoirs on fish population growth -- was applied in one stocked reservoir. Catch/effort and stocking data from Bargi reservoir (Madhya Pradesh, India) was used in model validation and in the estimation of Maximum Sustainable Yield (MSY). The present study not only be helpful to estimate impact, but also to understand how different stocking regimes impact the fish harvest potential at varying levels of efforts for effective fisheries management of reservoirs. [ABSTRACT FROM AUTHOR]

Temperature and seasonal rainfall along with other environmental variables are important in regulating the reproductive cycles in teleost fishes. Certain environmental variables may act as cues for reproduction and changes in these may affect seasonality and success of reproduction, as fishes are known to integrate their physiological functions with environmental cycles. Wetlands are sensitive to climate change due to their shallow and confined nature. Since wetlands are important spawning and nursery grounds for many fishes, changes in the environmental variables may have direct consequences for the spawning and survival of fish. In the present study, we have assessed climatic and water chemistry variables capable of influencing seasonality in environmental variables as well as gonadal maturation of spotted snakehead Channa punctata, to predict threshold values of Gonado Somatic Index in females and a favourable range of identified climatic and water chemistry variables for breeding success. Among the climatic and water chemistry variables studied, seasonal variation in rainfall was found to have the most profound effect on gonadal maturation and breeding in C. punctata, followed by water temperature. The favourable range of rainfall obtained varied between 800 mm to 1400 mm, corresponding to the water temperature range between 29 °C and 31 °C. An overall significant warming trend with a reduction in total rainfall has been observed with changes in seasonal trends in temperature and rainfall in the study area. The rainfall being the major climatic factors influencing water chemistry in the wetlands during the spawning season, changes in rainfall pattern may influence breeding periodicity of C. punctata in wetlands in climate change scenario. [ABSTRACT FROM AUTHOR]

It is envisaged that the influence of river hydrology on the commercial catch of anadromous species in river–estuary systems is very pronounced. We delineate a general framework to quantify the effect of changing river hydrology on the catch of a very unique anadromous species, the HilsaTenualosa ilisha, a shad species that is distributed across the Indo-Pacific region. The Narmada River–estuary system was studied as a representative for the region. Two synthesized factors—migratory habitat availability (factor 1) and migratory habitat quality potential (factor 2)—were derived from five hydrological variables to explain 96% of the variability in the hydrological regime of the Narmada River–estuary system. Based on the proposed autoregressive integrated moving average with explanatory variables (ARIMAX) modeling framework, we found that both factors had significant positive effects. Model-based forecasts of Hilsa catch revealed a decreasing trend in the future if a similar hydrological regime prevails. In a simulated future scenario, a 15% increase in Hilsa catch was predicted to occur in response to a 2% increase in factor 1, considering the last 5 years’ average catch as a benchmark. Some management strategies focused on controlling the hydrological regime are suggested to enhance Hilsa fisheries. Received February 15, 2017; accepted July 1, 2017 Published online October 11, 2017 [ABSTRACT FROM PUBLISHER]