Search

Your search keyword '"Nasim, Suhail"' showing total 17 results
Start Over Author "Nasim, Suhail"
17 results on '"Nasim, Suhail"'

4. High incidence of point mutation in K-ras codon 12 in carcinoma of the fallopian tube

Author

Mizuuchi, Hidemitsu, Mori, Yasuhiro, Sato, Kenichiro, Kamiya, Hirohumi, Okamura, Naoki, Nasim, Suhail, Garrett, Carleton T., and Kudo, Ryuichi

Subjects
Uterine tumors -- Genetic aspects, Ras genes -- Abnormalities, Health
Abstract

Background. Adenocarcinoma of the fallopian tube is a rare tumor with a poor prognosis. Whether these carcinomas possess any genetic changes that contribute to their malignant behavior is unknown and to date few studies regarding the molecular pathogenesis of these tumors have been reported. In adenocarcinoma of the endometrium, mutations in the first exon of K-ras, although relatively infrequent, were observed to be an independent risk factor for poor clinical outcome. Methods. Eight patients with adenocarcinoma of the fallopian tube were examined for mutations in the 12th codon of K-ras. DNA was obtained from single sections of paraffin embedded tumor tissue and the first exon of K-ras was amplified by the polymerase chain reaction. Point mutations were assayed using a nonradioactive restriction fragment length polymorphism technique. Results. The eight patients in this study varied in clinical stage from I-IV and were all treated with surgery and chemotherapy. Six of eight of the patients died and one of the surviving patients had metastases in the vertebrae. K-ras point mutations were detected at codon 12 in seven of the eight tumors (87.5%). Conclusions. K-ras mutations occurred with high frequency in this series of eight patients with fallopian tube carcinoma, suggesting that mutations of this protooncogene could play an important role in the molecular pathogenesis of this lesion. Cancer 1995; 76:86-90. Key words: ras, point mutation, carcinoma, fallopian tube.

Published
1995

5. Cardiac myocytes and dendritic cells harbor human immunodeficiency virus in infected patients with and without cardiac dysfunction: detection by multiplex, nested, polymerase chain reaction in individually microdissected cells from right ventricular endomyocardial biopsy tissue

Author

Rodriguez, E. Rene, Nasim, Suhail, Hsia, Judith, Sandin, Ramon L., Ferreira, Andrea, Hilliard, Betty A., Ross, Allan M., and Garrett, Carleton T.

Subjects
HIV (Viruses) -- Identification and classification, Heart cells, Muscle cells, Health
Abstract

Cardiovascular disease occurs in 6.5 to 6.8 percent of patients infected with the human immunodeficiency virus (HIV). In many cases, this cardiovascular disease is the result of some opportunistic infection, which may be viral, bacterial, protozoal, or fungal. However, in some cases the cardiovascular disease seems to be the result of intrinsic changes in the heart muscle itself. Some studies have claimed to find HIV within the heart muscle cells, while other studies have failed to confirm this. The current study used the sensitive polymerase chain reaction to detect the presence of HIV in cells dissected from biopsy specimens of HIV-infected patients. Fifteen patients were enrolled in the study. Five of these patients had symptoms that suggested the presence of a heart abnormality, while the remaining 10 patients did not. Biopsies were taken from the inside of the heart and were dissected to separate the myocytes, or muscle cells, from the dendritic (nerve) cells of the heart muscle. Gene sequences indicative of the presence of HIV could be confirmed in 2 of the 5 myocyte specimens taken from patients with cardiac symptoms and in 6 of 10 patients without symptoms. Similarly, HIV could be detected in the dendritic cells of all 5 symptomatic patients and in 8 of the 10 patients without symptoms. These results suggest that the heart cells themselves are infected with HIV in many HIV-infected patients. However, infection with HIV does not seem to be correlated with heart symptoms, suggesting that some other factor in addition to HIV is necessary for the development of symptomatic heart abnormalities. (Consumer Summary produced by Reliance Medical Information, Inc.)

Published
1991

16. Overexpression of telomerase-associated chaperone proteins in prostatic intraepithelial neoplasia and carcinomas.

Author

Elmore LW, Forsythe R, Forsythe H, Bright AT, Nasim S, Endo K, and Holt SE

Subjects
Adenocarcinoma metabolism, Adenocarcinoma secondary, Aged, Aged, 80 and over, Cell Nucleus metabolism, Humans, Immunoenzyme Techniques, Male, Middle Aged, Neoplasm Staging, Prognosis, Prostaglandin-E Synthases, Prostate metabolism, Prostate pathology, Prostatic Hyperplasia metabolism, Prostatic Hyperplasia pathology, Prostatic Intraepithelial Neoplasia pathology, Prostatic Neoplasms pathology, HSP90 Heat-Shock Proteins metabolism, Intramolecular Oxidoreductases metabolism, Prostatic Intraepithelial Neoplasia metabolism, Prostatic Neoplasms metabolism, Telomerase metabolism
Abstract

Over 90% of prostate cancers express telomerase activity. In an experimental model, hsp90 and p23, which are necessary for telomerase assembly and function, dramatically increase during tumorigenic conversion. We immunohistochemically analyzed 60 prostate carcinomas, 50 prostatic intraepithelial neoplasias (PIN) and 25 benign prostatic tissues to determine whether hsp90/p23 expression correlates with advancing stage and whether chaperone distribution overlaps with hTERT, the catalytic component of telomerase. Strong expression of hsp90/p23 was detected in approximately 95% of PIN and carcinomas without relationship to Gleason score. While hsp90/p23 immunostaining was predominantly diffuse and cytoplasmic, nuclear immunoreactivity was observed in several moderate-to-high grade carcinomas, and those carcinomas with nuclear chaperone staining exhibited detectable hTERT. Our data suggest enhanced chaperone-mediated telomerase assembly as a mechanism for increased activity in advanced prostate carcinomas, stable association between chaperones and telomerase in vivo, and utility for chaperone immunostaining to identify focal PIN in the context of widespread hyperplasia.

Published
2008

17. Normal, benign, preneoplastic, and malignant prostate cells have distinct protein expression profiles resolved by surface enhanced laser desorption/ionization mass spectrometry.

Author

Cazares LH, Adam BL, Ward MD, Nasim S, Schellhammer PF, Semmes OJ, and Wright GL Jr

Subjects
Adult, Aged, Biomarkers, Tumor metabolism, Humans, Hyperplasia metabolism, Logistic Models, Male, Middle Aged, Prostatic Intraepithelial Neoplasia metabolism, Protein Array Analysis, Proteins analysis, Sensitivity and Specificity, Mass Spectrometry methods, Prostatic Neoplasms metabolism, Prostatic Neoplasms pathology
Abstract

Purpose: The objective of this study was to discover protein biomarkers that differentiate malignant from nonmalignant cell populations, especially early protein alterations that signal the initiation of a developing cancer. We hypothesized that Surface Enhanced Laser Desorption/Ionization-time of flight-mass spectrometry-assisted protein profiling could detect these protein alterations., Experimental Design: Epithelial cell populations [benign prostatic hyperplasia (BPH), prostate intraepithelial neoplasia (PIN), and prostate cancer (PCA)] were procured from nine prostatectomy specimens using laser capture microdissection. Surface Enhanced Laser Desorption/Ionization-time of flight-mass spectrometry analysis was performed on cell lysates, and the relative intensity levels of each protein or peptide in the mass spectra was calculated and compared for each cell type., Results: Several small molecular mass peptides or proteins (3000-5000 Da) were found in greater abundance in PIN and PCA cell lysates. Another peak, with an average mass of 5666 Da, was observed to be up-regulated in 86% of the BPH cell lysates. Higher levels of this same peak were found in only 22% of the PIN lysates and none of the PCA lysates. Expression differences were also found for intracellular levels of prostate-specific antigen, which were reduced in PIN and PCA cells when compared with matched normals. Although no single protein alteration was observed in all PIN/PCA samples, combining two or more of the markers was effective in distinguishing the benign cell types (normal/BPH) from diseased cell types (PIN/PCA). Logistic regression analysis using seven differentially expressed proteins resulted in a predictive equation that correctly distinguished the diseased lysates with a sensitivity and specificity of 93.3 and 93.8%, respectively., Conclusions: We have shown that the protein profiles from prostate cells with different disease states have discriminating differences. These differentially regulated proteins are potential markers for early detection and/or risk factors for development of prostate cancer. Studies are under way to identify these protein/peptides, with the goal of developing a diagnostic test for the early detection of prostate cancer.

Published
2002

Catalog

Books, media, physical & digital resources
See catalog results