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Abstract Developing sustainable and eco-friendly methods for nanoparticle (NP) production in an era of environmental consciousness is crucial. This study introduces a novel approach to synthesizing silver (Ag) NPs using Citrus paradisi leaves extract (CPLE) as a green precursor at optimum conditions of the AgNO3 (2 mM) with CPLE in 1:3 ratio, at pH 14 and 80 °C temperature for reaction time of 4 h. The CP@AgNPs were formed and stabilized by Naringen, a major Citrus paradisi component. CP@AgNPs were thoroughly characterized through ultraviolet−visible (UV−vis) and Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) analysis, and field emission scanning electron microscopy (FE-SEM) imaging techniques. CP@AgNPs demonstrated SPR peak at 450 nm, face cubic crystal structure, the average size of 8 nm, rod-shaped particle adsorbed on quasi-spherical shaped agglomerated NPs, significantly impacting both environmental and biomedical fields. In the catalytic degradation experiment, an application for environment pollutant reducer, CP@AgNPs, achieved an impressive 85% degradation efficiency of the methyl orange (MO) dye, showcasing their potential as a sustainable solution for wastewater treatment. Additionally, CP@AgNPs exhibited potent anti-biofilm properties, with half maximal inhibitory concentration (IC50) values of 0.13 and 0.12 mg/ml against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), respectively, indicating their promise in addressing biofilm-related issues in healthcare and industrial settings. CP@AgNPs also displayed exceptional antioxidant potential with IC50 values of 2.02, 0.07, and 0.035 mg/ml for CPLE, CP@AgNPs, and ascorbic acid, respectively, in scavenging DPPH radical, suggesting their utility in biomedical applications for mitigating oxidative stress. Notably, the bio-activity results of CP@AgNPs surpassed those of CP leaf extract, highlighting the enhanced properties achieved through this green synthesis approach. This study provides a sustainable and environmental remediation to biomedical science.

Abstract Subarachnoid hemorrhage (SAH) is the deadliest form of hemorrhagic stroke; however, effective therapies are still lacking. Perfluorocarbons (PFCs) are lipid emulsion particles with great flexibility and their much smaller size as compared to red blood cells (RBCs) allows them to flow more efficiently within the blood circulation. Due to their ability to carry oxygen, a specific PFC-based emulsion, PFC-Oxygent, has been used as a blood substitute; however, its role in cerebral blood flow regulation is unknown. Adult C57BL/6 wildtype male mice were subjected to an endovascular perforation model of SAH followed by an intravenous (i.v.) injection of 9 ml/kg PFC-Oxygent or no treatment at 5 h after SAH. At 48 h after SAH, functional and anatomical outcomes were assessed. We found that SAH resulted in significant neurologic and motor deficits which were prevented by PFC-Oxygent treatment. We found that SAH-induced vasospasm, reduced RBC deformability, and augmented endothelial dysfunction were also restricted by PFC-Oxygent treatment. Moreover, mitochondrial activity and fusion proteins were also markedly decreased as assessed by oxidative phosphorylation (OXPHOS) after SAH. Interestingly, PFC-Oxygent treatment brought the mitochondrial activity close to the basal level. Moreover, SAH attenuated the level of phosphorylated AMP-activated protein kinase (pAMPK), whereas PFC treatment improved pAMPK levels. These data show the beneficial effects of PFC-Oxygent in limiting the severity of SAH. Further studies are needed to fully understand the mechanism through which PFC-Oxygent exerts its beneficial effects in limiting SAH severity.

To identify a promising alternative to lead-based materials for solar cell application, we investigated the different physical properties of K2ScCoX6 (X = F, Cl) perovskites. Both materials have ferromagnetic ground state. The obtained optimize lattice constants are found to be 8.48 Å and 10.04 Å for K2ScCoF6 and K2ScCoCl6 respectively. Our finding indicate that these materials exhibit excellent structural, mechanical, the thermal stability, as evidenced by their Goldsmith's tolerance factor, elastic parameters, and negative formation energies. The formation energy is found to be -2.4 and -2.1 eV/atom for K2ScCoF6 and K2ScCoCl6 respectively. The electronic properties reveals that both materials have semiconducting nature. Notably, we observed low direct bandgap of 0.93 eV for K2ScCoF6 and 1.22 eV for K2ScCoCl6, which contrast with the typically large bandgap values reported for most halide double perovskite. The calculated values of Poisson's and Pugh's ratios, along with positive Cauchy's pressure, suggest a ductile nature for these compounds. Additionally, the optical properties show high absorption and optical conductivity, coupled with low reflectivity and minimal energy loss in lower energy ranges. These results suggest that the halogen-based double perovskite materials have significant potential as photovoltaic absorber materials in solar cell applications. Furthermore, their higher Seebeck coefficients, power factors and low thermal conductivity at room temperature underscore their potential for thermoelectric applications.

Sir Syed Ahmad Khan is considered well known intellectual of India and he served as most remarkable educational reformer during Colonial era. Sir Syed considered an important Muslim leader in India who had good collaboration with English Statesmen after 1857. With socio-political contribution, Syed Ahmad Khan, who had profound a political insight presented his religious ideas. Although his religious concepts created different conspiracies among the Muslims of India, especially the religious class opposed his religious theories and declared these stance against the spirit of Islam. This paper is an analytical study about the religious thoughts of Sir Syed which critically explore the religious thoughts of Sir Syed Ahmad Khan.

Preaching of Islam is the most important responsibility of Ummah. For the payment of this duty various missionary organizations were established in the Muslim world. Tablighi Jammat is also a Tablighi movement, which was established by Maulana Muhammad Ilyas. Because the Maio nation was in danger of apostasy, this requires a Tablighi Jammat occurred. Now this movement is active in many countries of the world. The group has set some principles. They speech according to these principles, it's also called six numbers. The first number is Kalima-e-Tayyaba which is the first fundamental principle of Islam. The second is Prayer which is the second fundamental principle of Islam, the third is Knowledge and Ziker, the fourth is honor of Muslim , the fifth is pure believe, the sixth is Dawat-o-Tabligh. These six numbers were not in Muhammad (PBUH)'s life, but act on these principles is also beneficial for Muslims.

Preaching of Islam is the most important responsibility of Ummah. For the payment of this duty various missionary organizations were established in the Muslim world. Tablighi Jammat is also a Tablighi movement, which was established by Maulana Muhammad Ilyas. Because the Maio nation was in danger of apostasy, this requires a Tablighi Jammat occurred. Now this movement is active in many countries of the world. Tablighi Jammat has own ways and timing course for preaching. They patrol in homes and various areas.Besides them going for three days, which is called Sahroza. They are going to preach forty days which is called Chilah. They are also going to seven months and a year. Their annual Jor is also held every year in the Ray Wind. The deputations send to entire country and outside countries for preaching. Besides male parties female parties also send for preaching, but according to Islamic principles. On the other hand they have their own activities of praeching. There are also benefits of time course and activities for preacher. The preachers are become bound by time. He may be escape from large evils like lie, insults, adultry, slander etc.

The Prophet Mohammad (SAW) is acknowledged as the greatest mentor of all times. A lot of research work with blend of devotion and dedication has so far been under taken several research scholars in the area of Seerah writings. It is the miraculous feature of noblest Personality of Prophet Mohammad (SAW) that the followers of different religions, irrespective of religious bindings have produced master piece of writing, in this context every contributor tried to imbibe and disseminate pearls of knowledge, affection and sacred attachment with Prophet of Islam (SAW) according to their capacity and potential. Among the shining stars of Seerah Writing the contribution of Allama Shibli Numani has been acknowledged as the great pioneer Seerah Writer. He collected abundant of data about Seeraat-un-Nabi (SAW) and wrote a book '' Seerat-un-Nabi (SAW) in Urdu. In this article very important discussions of Seerah Writing in'' Seerat-un-Nabi'' were taken into consideration like importance of Seerah Writing, starting of Seerah Writing, discussion on of Seerah Arts, European publications and their joint principles.

Today, there is the roar of sustainable material development around the globe. Green nanotechnology is one of the extensions of sustainability. Due to its sustainable approach, the green fabrication of nanoparticles has recently surpassed their classical synthesis in popularity. Among metal nanoparticles, contemporary findings have demonstrated that bimetallic nanoparticles possess more potential for different applications than monometallic nanoparticles due to the synergistic effects of the two metals. So, we are presenting facile, one-vessel, and one-step phyto-fabrication of Ag–Fe BMNPs using the bud extract of Syzygiumaromaticum. The synthesized nanoparticles were characterized by UV-VIS, XRD, EDX, FTIR, and SEM. The synthesized NPs and the extract underwent biological studies. The radical scavenging potential of the NPs and the extract was found to be 64% and 73%, and the insecticidal potential was found to be 80% and 100%, respectively. Similarly, the NPs and the extract both exhibited good antibacterial activity. The zone of inhibition using 100 mg/mL of extract and NPs was found to be 1 cm against all bacterial species, i.e., K. pneumonia, E. coli, and S. aureus. It was 1.5 cm, 1.3 cm, and 1 cm against K. pneumonia, E. coli, and S. aureus, respectively, showing that the antibacterial activity of the extract is higher than that of the NPs. So, this study unlocks the synthesis of Ag–Fe bimetallic nanoparticles using eco-safe, cost-effective, facile, and least-harmful green methodology with potential applications of both NPs and SA extract in medical and agricultural fields, a step towards sustainability.

Traditional microbial diagnostic methods face many obstacles such as sample handling, culture difficulties, misidentification, and delays in determining susceptibility. The advent of artificial intelligence (AI) has markedly transformed microbial diagnostics with rapid and precise analyses. Nonetheless, ethical considerations accompany AI adoption, necessitating measures to uphold patient privacy, mitigate biases, and ensure data integrity. This review examines conventional diagnostic hurdles, stressing the significance of standardized procedures in sample processing. It underscores AI’s significant impact, particularly through machine learning (ML), in microbial diagnostics. Recent progressions in AI, particularly ML methodologies, are explored, showcasing their influence on microbial categorization, comprehension of microorganism interactions, and augmentation of microscopy capabilities. This review furnishes a comprehensive evaluation of AI’s utility in microbial diagnostics, addressing both advantages and challenges. A few case studies including SARS-CoV-2, malaria, and mycobacteria serve to illustrate AI’s potential for swift and precise diagnosis. Utilization of convolutional neural networks (CNNs) in digital pathology, automated bacterial classification, and colony counting further underscores AI’s versatility. Additionally, AI improves antimicrobial susceptibility assessment and contributes to disease surveillance, outbreak forecasting, and real-time monitoring. Despite a few limitations, integration of AI in diagnostic microbiology presents robust solutions, user-friendly algorithms, and comprehensive training, promising paradigm-shifting advancements in healthcare.

Neurodegenerative diseases, such as Alzheimer’s disease (AD), Parkinson’s disease (PD), amyotrophic lateral sclerosis (ALS), Huntington’s disease (HD), stroke, and aneurysms, are characterized by the abnormal accumulation and aggregation of disease-causing proteins in the brain and spinal cord. Recent research suggests that proteins linked to these conditions can be secreted and transferred among cells using exosomes. The transmission of abnormal protein buildup and the gradual degeneration in the brains of impacted individuals might be supported by these exosomes. Furthermore, it has been reported that neuroprotective functions can also be attributed to exosomes in neurodegenerative diseases. The potential neuroprotective functions may play a role in preventing the formation of aggregates and abnormal accumulation of proteins associated with the disease. The present review summarizes the roles of exosomes in neurodegenerative diseases as well as elucidating their therapeutic potential in AD, PD, ALS, HD, stroke, and aneurysms. By elucidating these two aspects of exosomes, valuable insights into potential therapeutic targets for treating neurodegenerative diseases may be provided.

In this article, a detailed overview has been presented about the prefigurement of arrival of Holy Prophet Muhammad (PBUH) in the light of Quran. The study of this article is related to arrival of Holy Prophet Muhammad (PBUH) according to Quranic arguments. Quran says that the arrival of Holy Prophet Muhammad (PBUH) is the result of Hazrat Ibraheem (A.S) Prayer. Besides other reveled Holy books also described the prefigurement of arrival of Holy Prophet Muhammad (PBUH). The methodology of this research is inductive where the data has been collected from original and secondary sources and then has been discussed in a scholastic way.

Background/Objective: Rotavirus is the main reason of diarrhea with gastroenteritis during childhood. This study aims to identify the demographical and clinical features of rotavirus (RV) suspected cases at Al-Baha Region. Materials and Methods: This is a cross-sectional study, it was conducted on selected 298 suspected cases with RV gathered from three noteworthy hospitals in Al-Baha area (King Fahd Hospital, Prince Meshari Hospital, and Aqiq General Hospital). The selected cases had been included based on case definition of the World Health Organization for rotavirus gastroenteritis. The data were collected from existing data in the selected hospitals' records, it was then analyzed using SPSS software. Results: The findings of this study expressed that male children are somewhat more than females (55% to 45%), the majority of them are under 2 years and most commonly are at infancy period (53.4%) and the greater part of them were admitted to Aqiq General Hospital (56.4%). The study showed that the clinical features of the hospitalized children with suspected rotavirus as 65.5% had stool motions at least twice a day, about 66.5% had no vomiting, while 59.1% with mild dehydration and 30.5% with moderate dehydration, 42.3% with lethargy, 35.2% with irritability, 53.4% with sunken eyes, and 48.3% unable to drink water. The children of the age group (≤2 years old) had significantly severe dehydration and diarrhea and were more febrile compared to other children. Conclusion: The rotavirus is a major reason of diarrhea with gastroenteritis among under 5 years children in Al-Baha region. Rotavirus suspected cases have been featured by severe diarrhea, vomiting, fever, and mild dehydration among suspected case in the governmental hospitals at Al-Baha region.

The concentrations of Cd, Cr, Cu, Fe, Ni, Pb, & Zn were determined in the samples of soil, dismantling dust and rubber collected from the electronic waste dumping site of Shershah market and rubber from the Local market of Karachi city Pakistan. The city e-waste dumping and dismantling sites toxicity were not conducted before using modern techniques. The subsequent data of the heavy metals concentration were obtained using Atomic Absorption Spectrometry (AAS), Scanning Electron Microscopy (SEM), and Wavelength Dispersive- X-Ray Fluorescence Spectrometry (WD-XRF). Cu, Pb, and Zn were found as 133.17, 104.53 & 113.26 in soil, whereas in dismantling dust Pb & Zn were remained 10.56 and 12.65 mg/kg similarly. The China toy particle analysis by SEM was resulting metallic trend as Fe > Zn > Pb > Cd > Ni > Cr. The estimated data were compared with the levels allowed by the Pakistan Environmental Protection Agency (Pak-EPA) and the United States Environmental Protection Agency (USEPA). The maximum allowable limit for Cd is 3; Cr, Cd, and Pb are 100 for Ni is 50 and 300 mg/kg for Zn. The presence of these heavy metals from e-waste dumping would become soon a significant reason to cause serious health problems for the nearby residents and as well as city too. It has been concluded that the dumping of e-waste is the major source of contamination of heavy metals in the studied media. It is recommended that the e-waste must be recycled formally to prevent the soil from being polluted. .

Marriage is a bond that ties two individuals into a conjugal relationship. This relationship is vital in Islam since it makes a family, a family changes over into a clan and ultimately the clan moves in the society. Islam appends extraordinary significance to this bond, so simplicity is liked in this relationship. The aim of this research was to examine the marriage customs in the Peshawar region. The investigation uncovered that the vast majority in the Peshawar region of Khyber Pakhtunkhwa is embracing non-Shariah ceremonies at weddings. The ceremonies of marriage functions in the Peshawar region are received from Hindu traditions. For the assortment of information, the statistical technique was chosen and 200 samples were made, each questionnaire had ten questions. These samples were distributed among the people (males and females) randomly and gathered perspectives were shown through different measurable tables and diagrams. The most perspectives on individuals were that the Peshawar region is embracing non-Shariah customs in wedding rituals. It is an explanation of the ignorant of Islamic teachings. With the progression of time, individuals are moving towards destruction because of less knowledge about Islam. In this manner, the desperate need of it that Islamic teachings ought to be followed and simplicity ought to be embraced in wedding rituals. This exploration paper can be helpful and informative in its particular field for researchers and readers.

BackgroundFOXO3, a member of the FOX transcription factor family, is frequently described as being deregulated in cancer. Additionally, notable role of FOXO3 can be easily recognized in the process of ageing and survival. Even though various studies have been done to acknowledge the tumour-suppressive or oncogenic role of FOXO3 in cancer, still there exist a lack of understanding in terms of cancer prognosis and treatment. Therefore, to provide better insight, our study aims to evaluate the role and function of FOXO3 in breast cancer in Indian female patients. We examined the FOXO3 expression levels in breast cancer samples by analyzing mRNA and protein expression along with its clinicopathological parameters.ResultsA total of 127 cases of breast cancer with equal normal cases (n=127) were assessed with methylation (MS-PCR), Immunohistochemistry (IHC), mRNA expression using Real-time PCR was analysed and 66.14% cases at mRNA level were found to be downregulated, while 81.10% of cases had little or very little protein expression. Our data state, the promoter hypermethylation of the FOXO3 gene and the downregulated protein expression are significantly correlated (p=0.0004). Additionally, we found a significant correlation between the level of FOXO3 mRNA with ER (p=0.04) and status of lymph node (p=0.01) along with this.ConclusionData suggests the prognostic significance and the tumour-suppressive role of FOXO3 in breast cancer cases studied in India. However, there is a need for the extended research targeting FOXO3 to measure its clinical potential and develop well-defined therapeutic strategies.

Backbiting is an unethical disease that is at the forefront of society's propagation of immorality. And it's largely women that are involved. It affected almost every family and individual. The sickness has spread so far that it has developed into a fistula. Islam teaches man how to develop character and avoid moral blunders. Which destroys society while instilling love, compassion, kindness, and mercy in human souls. In this way, society's peace and order are restored. This article was drafted in the background of the fact that women in modern society play a larger role in backbiting than men, and their loss is the only thing that can destroy not just society but also many homes. This research paper was written with Islamic teachings in mind, so that future readers would be able to determine what is the legal status of backbiting in Islam after reading it. So that mankind understands that a backbiter is deserving of punishment.

Objective: To evaluate the effect of different preparations of fluoride gels on the salivary pH of albino rats. Material and Methods: This experimental study consisted of 40 Albino rats randomly divided into four equal groups. Group A was the control group and received no intervention. Experimental group B received a topical application of 0.2% sodium fluoride gel. Experimental group C received topical application of stannous fluoride gel 0.4%. Experimental group D received topical application of APF gel (1.23% acidulated phosphate fluoride gel). The different preparations of the gels were applied once daily for 4 minutes on the occlusal surface of the right maxillary molars for 14 days. Salivary pH values were recorded immediately after the application of gels with the help of pH paper on day 1 and day 14. Results: There was a significant difference in the pH level of groups B, C and D after 14 days of fluoride application (p < 0.05). The non-parametric Kruskal Wallis test was applied for the comparison between the groups. Conclusion: This study concluded that all the fluoride gels after administration caused the acidic pH of saliva with the most acidic effect produced by APF gel.

Marriage is a bond that ties two individuals into a conjugal relationship. This relationship is vital in Islam since it makes a family, a family changes over into a clan and ultimately the clan moves in the society. Islam appends extraordinary significance to this bond, so simplicity is liked in this relationship. The aim of this research was to examine the marriage customs in the Peshawar region. The investigation uncovered that the vast majority in the Peshawar region of Khyber Pakhtunkhwa is embracing non-Shariah ceremonies at weddings. The ceremonies of marriage functions in the Peshawar region are received from Hindu traditions. For the assortment of information, the statistical technique was chosen and 200 samples were made, each questionnaire had ten questions. These samples were distributed among the people (males and females) randomly and gathered perspectives were shown through different measurable tables and diagrams. The most perspectives on individuals were that the Peshawar region is embracing non-Shariah customs in wedding rituals. It is an explanation of the ignorant of Islamic teachings. With the progression of time, individuals are moving towards destruction because of less knowledge about Islam. In this manner, the desperate need of it that Islamic teachings ought to be followed and simplicity ought to be embraced in wedding rituals. This exploration paper can be helpful and informative in its particular field for researchers and readers.

Backbiting is an unethical disease that is at the forefront of society's propagation of immorality. And it's largely women that are involved. It affected almost every family and individual. The sickness has spread so far that it has developed into a fistula. Islam teaches man how to develop character and avoid moral blunders. Which destroys society while instilling love, compassion, kindness, and mercy in human souls. In this way, society's peace and order are restored. This article was drafted in the background of the fact that women in modern society play a larger role in backbiting than men, and their loss is the only thing that can destroy not just society but also many homes. This research paper was written with Islamic teachings in mind, so that future readers would be able to determine what is the legal status of backbiting in Islam after reading it. So that mankind understands that a backbiter is deserving of punishment.

Honesty is a good attribute and a blessing of Almighty Allah. Almighty Allah gives this attribute to whomever He wills. Honesty and integrity are the best qualities. Islam is the religion of peace and security, so it wants a peaceful society. Islam dislikes dishonesty, betrayal, deception, corruption, lies, and deception. Islam has pointed out the right and wrong paths and now it is the responsibility of a person to choose one of these paths, whether he adopts the good path or the bad path. But it is a fact that the people of a society who become virtuous and honest, spend their lives with prosperity and peace in society. If we talk about contemporary society, it is a fact that we are going to downfall with time. Because we have ignored the teachings of Islam due to which we see everywhere in society unethical values, i.e., dishonesty, robbery, lies, betrayal, kidnapping, murder, violence, corruption, etc. The progress and prosperity of a society depend upon people’s honesty and integrity, when people will be honest then the environment of society will be peaceful. This research work aims to explain that honesty plays an important role in the establishment of a peaceful society.

Introduction: Maternal isoimmunization, also called alloimmunization, occurs when immune system of a pregnant female is sensitized to foreign RBC surface antigen producing immune process. This maternal blood when goes to fetal circulation, causes immune reaction and disease in case of maternal and fetal blood group differences. Aims & Objectives: To find out the frequency and types of red cell alloantibodies among females presenting to antenatal care and recognize risk factors for alloimmunization. Place and duration of study: This cross-sectional descriptive study was carried out at Antenatal Clinic of Shaikh Zayed Hospital, Lahore from 1st January 2013 to 31st August 2013. Material & Methods: Pregnant females with at least one previous pregnancy were typed for ABO and Rh antigens. They were screened and typed for red cell alloantibodies. Detailed history was taken to explore for the risk factors. SPSS version 20.0 was used for data analysis, frequencies of different alloantibodies, blood groups and risk factors reported as percentages, age and gravidity in mean±s.d. Results: Out of 200 enrolled cases, 6(3%) had alloantibodies. Of the positive cases, anti-D was found in 3(50%), anti-C in 2(33.33%) and anti-Kell in 1(16.67%). Commonest risk factors were history of peripartum hemorrhage and gynecological procedures. In Rh-negative cases, disparity of spouse Rh group was also main factor. Conclusion: The most common culprit antibody for alloimmunization was anti-D followed by anti-Kell and anti-C. Commonest risk factor for alloimmunization was pregnancy related bleeding and gynecological procedures. Large population-based studies are required to assess true magnitude of the problem.

Background: CTCF encodes 11-zinc finger protein which is implicated in multiple tumors including the carcinoma of the breast. The Present study investigates the association of CTCF mutations and their expression in breast cancer cases. Methods: A total of 155 breast cancer and an equal number of adjacent normal tissue samples from 155 breast cancer patients were examined for CTCF mutation(s) by PCR-SSCP and automated DNA sequencing. Immunohistochemistry (IHC) method was used to analyze CTCF expression. Molecular findings were statistically analyzed with various clinicopathological features to identify associations of clinical relevance. Results: Of the total, 16.1% (25/155) cases exhibited mutation in the CTCF gene. Missense mutations Gln > His (G > T) in exon 1 and silent mutations Ser > Ser (C > T) in exon 4 of CTCF gene were analyzed. A significant association was observed between CTCF mutations and some clinicopathological parameters namely menopausal status (p = 0.02) tumor stage (p = 0.03) nodal status (p = 0.03) and ER expression (p = 0.04). Protein expression analysis showed 42.58% samples having low or no expression (+), 38.0% with moderate (++) expression and 19.35% having high (+++) expression for CTCF. A significant association was found between CTCF protein expression and clinicopathological parameters include histological grade (p = 0.04), tumor stage (p = 0.04), nodal status (p = 0.03) and ER status (p = 0.04). Conclusions: The data suggest that CTCF mutations leading to its inactivation significantly contribute to the progression of breast cancer.

Background and Objective: Extrapulmonary tuberculosis occurring in breast is a rare disorder in West but is still present in developing nations where tuberculosis is endemic. Tuberculous Mastitis (TM) is difficult to diagnose as it has vague clinical symptoms and insufficient radiological findings. The objective of this study was to determine the frequency of tuberculous mastitis by accessible modalities for early diagnosis.Methods: After the Institutional Ethical Board approvals, Pathology laboratory archives and medical records of 5000 patients from Ibn-e-Sina Hospital Research Institute, Multan and Multi Test Laboratory, were retrieved from the year 2008 to 2018. A retrospective analysis of fine needle aspiration cytology (FNAC) results of patients who presented with breast lumps was carried out. The interpretation of cytological findings and Ziehl Neelsen stained smears was carried out by cytopathologists and microbiologists respectively. Mycobacterium DNA was detected using polymerase chain reaction (PCR) in selected cases.Results: Out of 5000 patients, tuberculosis was diagnosed in 264 (5.28%) cases on FNAC. Acid fast bacilli (AFB) positive smears were found in 79.5%patients while 20.45% cases were AFB negative. Among AFB negative smears, 38 cases were further analyzed by PCR, among which 32 (84.2%) cases showed PCR positive results for TM. An increased frequency of TM among all inflammatory breast lesions from 18% in 2008 to 47.19% in 2018 was observed.Conclusion: Tuberculous Mastitis is an uncommon form of extra-pulmonary tuberculosis. It has non- specific clinical, radiological and varied histological findings that often mimic breast abscess or carcinoma. FNAC is essential to guide the patient to further tests including histochemistry and PCR that can be used as more sensitive and time effective detection modalities.

Background: Forkhead box “O” one which is member of Forkhead box family of transcription factors is known to play key role in different physiological processes including cell cycle arrest, autophagy, and apoptosis. FOXO1 is defined to play tumor suppressive role in various malignancies including breast cancer and its Dysregulation is frequently reported. However, the evaluation of FOXO1 promoter methylation and its expression at mRNA and protein level in different stages of breast cancer and its association with different clinical parameters is still not studied. Therefore, for better understanding the role of FOXO1 in breast cancer, in our study we examined the FOXO1 mRNA and protein expression in Breast cancer samples of Indian breast cancer patients.Results: Total 127 breast cancer samples along with adjacent normal tissue (n = 127) were analyzed through methylation specific PCR (MS-PCR), mRNA expression (Real-time PCR) and Immunohistochemistry (IHC). We detected 69.29% cases to be downregulated at the mRNA level, and 77.95% of cases exhibited no or low protein expression. In our data we report a significant association (p = 0.0001) between the downregulated protein expression and promoter hypermethylation of FOXO1 gene. We also found a significant correlation of FOXO1 mRNA level with Age (p = 0.008), age at first live birth (p = 0,003), tumor size (p = 0.05) and lymph node status (p = 0.01).Conclusion: we in our study report the tumor suppressive role of FOXO1 in case of Indian breast cancer patients and our data suggest it to exhibit prognostic importance. However, further research is needed to evaluate FOXO1 significance in diagnostic and therapeutic targeting in breast cancer cases.

Background:FOXP3 gene, known to be a potential tumor suppressor, has been identified to interact with HER2 in mammary cancer. Moreover, the high expression of FOXP3 serves as a good predictor of the survival of patients in breast cancer, prostate cancer, and gastric cancer. The expression and epigenetic alterations were evaluated in female breast cancer patients.Material and Methods: Expression studies at the mRNA level and protein level were conducted in 140 breast cancer cases by real-time PCR and immunohistochemistry, respectively. Epigenetic studies were also conducted by analyzing the methylation status at the promoter region of the gene using MS-PCR.Results:FOXP3 mRNA expression and protein expression were downregulated in breast cancer patients. The absence of FOXP3 protein expression is significantly associated with promoter methylation, where 70 methylated cases exhibited protein loss (70/95, 73.6%). Statistically, we also found a significant correlation between FOXP3 protein expression and TNM stage, promoter methylation, and histological grade. The methylated FOXP3 cases that did not express protein were also significantly associated with positive lymph node metastasis and HER-2 status.Conclusion: The expression profile of FOXP3 may serve as a prognostic factor. In short, FOXP3 may stand in the most crucial list of biomarkers for breast cancer, bringing compelling results in terms of treatment and management of the disease.

The first goal of education in Islam is to nurture the abilities of man completely so that he becomes a good citizen. So that he lives his life according not only as part of society but according to modern times and trends. All this so that society gets betterment through him.To achieves this goal the Development of curriculum of educational institutes has a central role. This is because after the teacher and student the curriculum is the most important pillar of education. Adding to that no step in education is complete without it. Curriculum creation is systematic process where changing needs of society and the individual can be focused upon. Thus curriculum creation is an integral part of an educational system where the curriculum is designed by looking at the curriculum goals.

The association of interleukin-6 (IL-6)-174G > C (rs1800795) single nucleotide polymorphism (SNP) with the risk of acquiring rheumatoid arthritis (RA) is a relevant issue because of conflicting and consensus lacking reports published in literature. We investigated IL-6-174G > C promoter polymorphism in 34 RA patients, attending a tertiary care hospital in north India. We also performed a meta-analysis, of the previously published studies reporting this genetic relationship, in overall population, and independently in Asian and Caucasian ethnicities to further elucidate this association. A total of 13 studies, including the current one, involving 3291 RA cases and 3812 controls were analyzed. Out of the 13 studies, 6 were from Asian, 6 from Caucasian and 1 from a mixed population. Our case-control study showed significant association of IL-6-174G > C SNP with increased RA risk: allelic (OR = 3.750, 95% CI = 1.800–7.813, p C SNP in overall population: allelic (OR = 1.650, 95% CI = 1.169–2.329, p = 0.004); homozygous (OR = 1.380, 95% CI = 0.906–2.101, p = 0.133); heterozygous (OR = 1.559, 95% CI = 1.001–2.428, p = 0.049); dominant (OR = 1.663, 95% CI = 1.078–2.567, p = 0.022); and recessive (OR = 1.366, 95% CI = 0.964–1.935, p = 0.079). Subgroup analysis also showed this polymorphism to be associated with increased RA risk in Asian population: allelic (OR = 3.724, 95% CI = 1.361–10.190, p = 0.010); dominant (OR = 3.823, 95% CI = 1.320–11.074, p = 0.013); and recessive (OR = 4.357, 95% CI = 1.634–11.623, p = 0.003), but not in Caucasian population. This meta-analysis shows that IL-6-174G > C SNP is significantly associated with increased RA risk in overall, and specifically in Asian population.

Antimicrobial resistance happens when microorganisms mutates in manners that render the drugs like antibacterial, antiviral, antiparasitic and antifungal, ineffective. The normal mutation process is encouraged by the improper use of antibiotics. Mutations leading to quinolone resistance occur in a highly conserved region of the quinolone resistance-determining region (QRDR) of DNA gyrAse and topoisomerase IV gene. We analyzed antibiotic resistant genes and single nucleotide polymorphism (SNP) in gyrA and parC genes in QRDR in 120 E. coli isolates (both diarrheagenic and non-pathogenic) recovered from fresh stool samples collected from children aged less than 5 years from Delhi, India. Antibiotic susceptibility testing was performed according to standard clinical and laboratory standards institute (CLSI) guidelines. Phylogenetic analysis showed the clonal diversity and phylogenetic relationships among the E. coli isolates. The SNP analysis depicted mutations in gyrA and parC genes in QRDR. The sul1 gene, responsible for sulfonamide resistance, was present in almost half (47.5%) of the isolates across the diseased and healthy samples. The presence of antibiotic resistance genes in E. coli isolates from healthy children indicate the development, dissemination and carriage of antibiotic resistance in their gut. Our observations suggest the implementation of active surveillance and stewardship programs to promote appropriate antibiotic use and minimizing further danger.

Angiotensin-converting enzyme (ACE) gene is indispensable for endothelial control and vascular tone regulatory systems, usually affected in Systemic Lupus Erythematosus (SLE). ACE insertion/deletion (I/D) polymorphism may influence the progress of SLE. Earlier studies have investigated this association without any consistency in results. We performed this meta-analysis to evaluate the precise association between ACE I/D polymorphism and SLE susceptibility. The relevant studies were searched until December, 2017 using Medline (PubMed), Google-Scholar and EMBASE search engines. Twenty-five published studies involving 3,308 cases and 4,235 controls were included in this meta-analysis. Statistically significant increased risk was found for allelic (D vs. I: p = 0.007; OR = 1.202, 95% CI = 1.052–1.374), homozygous (DD vs. II: p = 0.025; OR = 1.347, 95% CI = 1.038–1.748), dominant (DD+ID vs. II: p = 0.002; OR = 1.195, 95% CI = 1.070–1.334), and recessive (DD vs. ID+II: p = 0.023; OR = 1.338, 95% CI = 1.042–1.718) genetic models. Subgroup analysis stratified by Asian ethnicity revealed significant risk of SLE in allelic (D vs. I: p = 0.045; OR = 1.238, 95% CI = 1.005–1.525) and marginal risk in dominant (DD+ID vs. II: p = 0.056; OR = 1.192, 95% CI = 0.995–1.428) models; whereas, no association was observed for Caucasian and African population. Publication bias was absent. In conclusion, ACE I/D polymorphism has significant role in overall SLE risk and it can be exploited as a prognostic marker for early SLE predisposition.