Search

This case describes a rare case of ovarian leiomyoma associated with granulosa cell tumor and polycystic ovary and in a fifteen-year-old mongrel female dog.

Uma chimpanzé de 22 anos de idade foi necropsiada com histórico clínico de anorexia, vômitos freqüentes e desidratação conseqüentes à gastrite iatrogênica. Macroscopicamente, o útero apresentava-se aumentado de volume, com o lúmen totalmente ocluído por nódulos firmes, esbranquiçados e coalescentes que se estendiam para o miométrio. Histologicamente, os nódulos eram constituídos por leiomiócitos bem diferenciados dispostos em várias direções e com coloração característica pelo tricrômio de Gomori e Masson. Pela imunoistoquímica, as células neoplásicas apresentavam marcação forte e difusa de receptores para progesterona e estrógeno, assim como de actina alfa de músculo liso. Algumas células neoplásicas e o estroma apresentavam marcação para vimentina e poucas células neoplásicas foram positivas para MIB-1. Com base nas características mosrfológicas e imunoistoquímicas foi firmado o diagnóstico de leiomioma uterino.

Hemogram and serum biochemistry (aspartate aminotransferase, alkaline phosphatase and gamma glutamiltransferase, total protein, urea, creatinine, calcium and phosphorus) were performed weekly in five crossbreed bovine after consumption of a diet containing citrus pulp pellets (40%), for 43 days. Percutaneous hepatic biopsy and histologic evaluation were performed in each animal before and after consumption of the citrus pulp diet. Hemogram, the enzymes aspartate aminotransferase and gamma glutamiltransferase, urea and creatinine had normal levels at the end of the experiment. No histologic lesions were observed in liver samples before or after citrus pulp consumption. However, there was an increase of serum phosphorus and reduction of serum calcium (p

It was investigated the efficiency of two extracts of plants and one fraction of their properties against the local effects of bothropic envenomation. Bothrops alternatus venom (1.25µg) diluted in 100µl of sterile saline solution was inoculated (intradermally) into the shaved dorsal back skin of 30 New Zealand rabbits. The animals were divided in six groups receiving the following treatments: group I: subcutaneous application of Curcuma longa extract (1.0ml); group II: topic treatment of Curcuma longa hydroalcoholic extract (1.0ml); group III: topic application of ar-turmerone in vaseline (1.0g); group IV: topic application of Curcuma longa methanolic extract (1.0ml); group V: topic application of Calendula officinalis ointment (1.0g); group VI: topic application of saline (1.0ml). These treatments were done at 30 minutes, and at 2, 4, 24 and 72 hours after venom inoculation. Intensity of local edema, hemorrhagic halo and necrosis were evaluated until 168h after that. Additionally, seven days after the Bothrops venom inoculation, blood was collected from heart with and without EDTA (10%) for hemogram and biochemical parameters (total protein, blood urea nitrogen, creatinine, and fibrinogen) and all the animals were anesthetized, sacrificed by ether inhalation and submitted to necropsy. Fragments of tissues were taken for histopathological evaluation. The most efficient treatment for inhibition of edema, necrosis and local hemorrhage after Bothrops alternatus venom was the topic application of ar-turmerone. Investigou-se a eficácia do extrato de plantas no tratamento local do envenenamento botrópico. Veneno de serpentes Bothrops alternatus (1,25µg) diluído em 100µl de solução salina estéril foi inoculado (via intradérmica) entre as escápulas de 30 coelhos. Os animais foram divididos em seis grupos (tratamentos): grupo I: tratamento subcutâneo com extrato de Curcuma longa; grupo II: tratamento tópico com extrato hidroalcoólico de Curcuma longa; grupo III: tratamento tópico com ar-turmerone em vaselina; grupo IV: tratamento tópico com extrato metanólico de Curcuma longa; grupo V: tratamento tópico com pomada de Calendula officinalis e grupo VI: aplicação tópica de solução salina a 0,9% (Controle). Os tratamentos foram realizados 30 minutos, 2h, 4h, 24h e 72h após a inoculação do veneno. Foram avaliados intensidade de edema local (com paquímetro), halo hemorrágico (régua com gabaritos circulares) e presença de necrose. Sete dias após a inoculação do veneno botrópico (168h) foi coletado sangue do coração, com e sem EDTA, para realização de hemograma, dosagem plasmática de fibrinogênio e dosagens séricas de proteína total, uréia e creatinina. Após as coletas de sangue, todos os animais foram anestesiados, sacrificados com inalação pelo éter etílico e necropsiados. Fragmentos de pele foram retirados para avaliação histopatológica. Os resultados obtidos mostraram que o tratamento mais eficaz para inibição da evolução do edema, necrose e hemorragia local após envenenamento com Bothrops alternatus foi a aplicação tópica de ar-turmerone.

A foliculogênese ovariana foi estudada em ratas adultas Wistar, hipotireóideas na fase de metaestro-diestro. O hipotireoidismo foi induzido pela administração oral e diária de propiltiouracil (1mg/animal). As ratas eutireóideas receberam placebo. Após 120 dias de tratamento, foi colhido o plasma para dosagem de tiroxina livre, progesterona e estradiol após o que foram sacrificadas para colheita dos ovários, tubas e útero, para avaliação histomorfométrica e histoquímica. O hipotireoidismo reduziu significativamente o peso dos ovários e o número de folículos secundários e terciários e de corpos lúteos sem, no entanto, alterar a porcentagem de folículos atrésicos e o número de folículos primários e pré-ovulatórios. As células da granulosa dos folículos secundários das ratas hipotireóideas apresentavam núcleo pequeno com significativa redução do número de regiões organizadoras de nucléolo (NORs). Essas mudanças não alteraram os valores periféricos de estradiol e de progesterona. Houve redução significativa da espessura do endométrio, do número de glândulas endometriais e da altura do epitélio do infundíbulo. Ovarian folicullogenesis was studied in adult Wistar rats. The animals were hypothyroid in the metaestrus/diestrus phase of the estrous cycle. Hypothyroidism was induced by oral treatment with 1mg/animal of propylthiouracil. Euthyroid rats were kept under the same experimental conditions receiving placebo. After 120 days under treatment, all animals were killed and plasma was taken to assessing free thyroxine, progesterone, and estradiol. The ovaries, tubes and uterus were sampled for histomorphometric and histochemistry evaluation. Hypothyroidism significantly reduced the weight of the ovaries and the number of secondary and tertiary follicles, and corpora luteum. The number of atretic follicles as well as primary and pre-ovulatory follicles was not affected. Granulosa cells in the secondary follicles of treated rats had small nuclei with a reduced number of AgNORs. Significant reduction of the endometrium thickness, number of endometrial glands and the infundibulum epithelium height was observed.

Descrevem-se as características anatomopatológicas e histoquímicas de 124 tubas uterinas de porcas (matrizes) abatidas em matadouros, nas fases folicular e luteínica do ciclo estral. As tubas uterinas foram distribuídas em quatro grupos: LSP- fase luteínica sem patologia tubárica (n=45), LCP- fase luteínica com patologia tubárica (n=19), FSP- fase folicular sem patologia tubárica (n=45) e FCP- fase folicular com patologia tubárica (n=15). Para estudo histoquímico de mucossubstâncias foram utilizadas as colorações de periodic acid Schiff (PAS) com e sem digestão prévia pela amilase salivar e Alcian Blue (AB) em pH 2,5 e 0,4. Utilizou-se a coloração de Ninhidrina-Schiff para evidenciação de proteínas ricas em radicais -NH2. Foram observadas alterações em 34 tubas uterinas. Independente da fase do ciclo estral, as patologias mais comumente encontradas foram os cistos de parede e a metaplasia escamosa. Em menor número foram observados divertículo e adenomiose. A ampola e o infundíbulo foram os segmentos mais afetados. Na fase folicular, o infundíbulo apresentou maior intensidade de secreção de mucossubstâncias neutras e AB 2,5-positivas. Na ampola houve maior intensidade de secreção de mucossubstâncias AB 2,5-positivas, sulfomucinas e de proteínas ricas em radicais -NH2. As patologias não reduziram significativamente a secreção de mucossubstâncias nas regiões adjacentes em nenhum dos segmentos tubáricos, tanto na fase luteínica quanto na folicular. Nas áreas de metaplasia escamosa do epitélio tubárico e nos cistos de parede houve redução da secreção de mucossubstâncias neutras e ácidas. The goal of this study was to describe the anatomopathological and histochemical features of uterine tubes from 62 sexually mature sows, in both follicular and luteal phases. The animals were assigned into four groups as follows: the first (LSP) containing sows in the luteal phase without tube pathology (n=45), the second (LCP) with sows in the luteal phase with tube pathology (n=19), the third (FSP) with sows in the follicular phase without tube pathology (n=45) and the fourth (FCP) with sows in the follicular phase with tube pathology (n=15). PAS, Alcian Blue (pH 2.5 and 0.4), and Ninhidrina-Schiff stains were used for the histochemical study. Grossly, 34 uterine tubes presented morphological lesions. Wall cysts and squamous metaplasia were the changes found more frequently in both phases. Ampulla and infundibulum were the segments affected more frequently. In the follicular phase, the infundibulum had a higher secretion intensity of neutral mucosubstances, whereas the ampulla and the infundibulum had a higher secretion intensity of AB 2.5-positive mucosubstances. The ampulla had a higher intensity of sulphomucins and Ninhidrina-Schiff-positive secretions in the follicular phase. The morphologic changes did not correlate with reduction in secretion of mucosubstance in the adjacent areas of the uterine tube in both follicular and luteal phases. There was a reduction in secretion of neutral and acid mucosubstances in areas affected by squamous metaplasia and wall cysts.

The aim of this work was to perform a morphometric analysis of the uterine tube epithelium of sexually mature sows, with or without pathological changes. The sows were either in follicular or luteal phases. One hundred and twenty-four uterine tubes of sixty-two sows were obtained from slaughterhouses. Sections of the uterine tube, uterus and ovary were processed for morphometrical evaluation. The uterine tubes were divided into four groups. The first (LSP) group was composed by animals in luteal phase without pathological changes (n=45), the second (LCP) by animals in luteal phase with pathological changes (n=19), the third (FSP) by animals in follicular phase without pathological changes (n=45) and the fourth (FCP) by animals in follicular phase with pathological changes (n=15). In the follicular phase (FSP), the ampulla had a higher epithelium, followed by the infundibulum and the isthmus (P

The goal of this study was to determine whether the quantification of the nucleolar organizer regions (AgNORs) is a useful parameter for evaluation of the proliferative potential of non-neoplastic granulosa cells in ovarian follicles at different stages of development. Seven ovaries from six-month-old rats, all of which in metestrus-diestrus, were studied. The number of AgNORs from 60 granulosa cell nuclei was quantified in secondary follicles with two to three layers of cells and in follicles with more than five layers of granulosa cells. Follicles with two to three layers had larger AgNORs in lower numbers, whereas more developed secondary follicles the AgNORs were predominantly smaller, scattered throughout the nucleus, and in significantly higher numbers. In conclusion, quantification of the nucleolar organizer regions is an appropriate parameter for differentiation of the proliferative potential of non-neoplastic granulosa cells.

The influence of the estrous cycle on the thyroid gland was studied. Twenty one five-to-seven-month-old Wistar female rats were divided according to the stage of the estrous cycle in two groups: metaestrus-diestrus and proestrus-estrus. After gross inspection, the thyroids were weighed, sampled, and processed for staining with hematoxilyn-eosin. Seric concentrations of total T4, free T4, total T3, TSH, progesterone, and estradiol were measured. The values of estradiol were significantly higher in the proestrus-estrus stage. However, no significant differences in the plasmatic concentrations of progesterone, free T4, total T4 and TSH throughout the cycle were found. The results of the morphometric study of the thyroid did not indicate any significant differences between the groups. These findings suggest that there is no thyreotrophic effect of estrogen during the estrous cycle in rats.

The changes of red and white blood cells counts in intact and castrated rats with hyperthyroidism were analysed. A total of 108 five-month-old female Wistar rats, divided into four groups, were used as following: euthyroid castrated, euthyroid intact, hyperthyroid castrated and hyperthyroid intact. Nine animals of each group were killed at 30, 60 and 90 days to evaluate the red and white blood cells counts. The conclusion was that castration or hyperthyroidism did not affect both the red and white blood cells.

This report describes the pathological findings of a leiomyoma in the gall bladder of a female 11-year-old Doberman dog. The animal underwent euthanasia due to the poor prognosis of an osteosarcoma of the skull. Grossly, there was a firm smooth whitish nodule in the gall bladder with 0.5 centimeter in diameter. Histologically the nodule was composed of isomorphic and well differentiated leiomyocytes arranged in bundles. The diagnosis was confirmed by Van Gieson staining.

This report describes the pathological findings of a myxoma in the vulva of a seven-year-old German Shepherd bitch, with history of mass increase in the vulva. After surgery, tipical and microscopic aspects were detected.

O presente trabalho relata um surto de intoxicação pela aflatoxina B1 em 150 bovinos machos mestiços, provenientes de um rebanho de 1774 animais, em sistema de confinamento, ingerindo polpa cítrica peletizada comercial, 5kg por animal. Todos os animais foram avaliados clinicamente, e os 18 animais que morreram foram necropsiados e submetidos a exames histopatológicos. A intoxicação foi confirmada pela presença da aflatoxina B1 (5ppm) em amostras da polpa cítrica peletizada utilizada na propriedade e em fragmentos de fígado dos animais que morreram. The present work reports an outbreak of intoxication by aflatoxin B1 in 150 adult bovine male, males from a herd of 1774 animals, in confinement system, fed on commercial citrus pellet. All animals were evaluated clinicaly, and ones that died were necropsied and tissues submitted to histopathotology. The intoxication was confirmed by aflatoxin B1 presence in samples of the citrus pellet and in fragments of liver of the animals which died.