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Aim: To determine the relationship between Oral Health-related Quality of Life (OHRQoL) with Dentition Status and Treatment need among 12 year old school children of Dilsukhnagar, Hyderabad., Patients and Methods: A cross sectional study was carried out among 990 private school children aged 12 years old in Dilsukhnagar, Hyderabad city. OHRQoL was assessed by 16-item Child Perceptions Questionnaire (CPQ 11-14 ), and Oral hygiene was evaluated using Simplified Oral Hygiene Index (OHI-S), and Dentition status and Treatment need. The data was analyzed using Statistical Package for Social Sciences (SPSS) version 21.0. Study population proportions and mean scores were compared using Chi-square test, Student t- test, and Analysis of Variance (ANOVA). Spearman's analysis was done to determine correlation between CPQ 11-14 and its domain scores with OHI-S and Dentition status and Treatment Need based on gender. P < 0.05 was considered statistically significant., Results: In the present study, all domains of CPQ 11-14 i.e., Oral Symptoms (OS) (3.27 ± 2.5; P = 0.0001), Functional Limitation (FL) (1.48 ± 2.1; P = 0.02), Emotional Wellbeing (EW) (1.83 ± 2.5; P = 0.02) and Social Wellbeing (SW) (1.25 ± 2.02; P = 0.0002) showed significant gender difference, with males having higher mean score compared to females. The majority of the study population was caries free 789 (79.7%). In addition, the dental caries experience among males and females was around 20% (p = 0.92). When total mean DMFT and DT scores were compared based on gender, no statistically significant difference was noted though females showed higher mean score., Conclusion: The study results indicate that, there is a correlation between oral hygiene status and dentition status with quality of life., Competing Interests: None

Introduction: Mishri is one of the form of smokeless tobacco, which is a roasted, powdered preparation made by baking tobacco on a hot metal plate until it is uniformly black, after which it is powdered. It is noted that mishri use is more commonly used by the women of low socioeconomic status, hence the need was felt to conduct this study among women mishri users of slums. Also, the consequences of mishri use are little known, hence an effort is made to find out its ill-effect on oral health., Objective: To assess knowledge, attitude, and practice (KAP) among women using mishri regarding its effects on their oral and general health., Materials and Methods: A 6-month KAP study was conducted among 100 women who were using mishri. Snowball sampling was used. Oral examination of the participants was also done for oral potentially malignant disorders, such as leukoplakia, erythroplakia, oral submucous fibrosis, and hyperkeratinized pouch., Results: About 61% of the population used mishri for cleaning the teeth and others used it as quid; 0.85% of the total participants knew that the use of mishri may lead to precancerous lesions/conditions. Only 17% knew that mishri use can cause gum disease; 84% of the population was willing to quit the habit of using mishri., Conclusion: It is concluded that all the participants had poor knowledge. Attitude toward quitting mishri use was found to be good. About 4% of the participants reported about quitting the habit., Clinical Significance: There is need to create awareness regarding harmful effects of mishri usage in this particular area to improve oral health status.

The aim of this study was to develop and optimize silverleaf bioassay, esterase analysis and PCR-based techniques to distinguish quickly and reliably biotype B of the whitefly, Bemisia tabaci (Gennadius), from Indian indigenous biotypes. Zucchini and squash readily develop silverleaf symptoms upon feeding by the B biotype, but they are not readily available in Indian markets. A local pumpkin variety 'Big' was, therefore, used in silverleaf assay, which developed symptoms similar to those on zucchini and squash and can be used reliably to detect B biotype. Analysis of non-specific esterases of B and the indigenous biotypes indicated both quantitative and qualitative differences in esterase patterns. Two high molecular weight bands were unique to B biotype and they occurred in abundance. These esterases were used to develop quick and field-based novel detection methods for differentiating B from the indigenous biotypes. Development of these simple and cost-effective protocols has wider application as they can be potentially used to identify other agricultural pests. Mitochondrial cytochrome oxidase I gene sequences and randomly amplified polymorphic DNA (RAPD) polymorphisms, generated using the primer OpB11, were also found useful for detecting B. tabaci biotypes. A B biotype-specific RAPD band of 800 bp was sequenced, which was used to a develop sequence characterized amplified region (SCAR) marker. The SCAR marker involved the development of B biotype-specific primers that amplified 550 bp PCR products only from B biotype genomic DNA. Silverleaf assay, esterases, RAPDs or a SCAR marker were used in combination to analyse whitefly samples collected from selected locations in India, and it was found that any of these techniques can be used singly or in combination to detect B biotype reliably. The B biotype was found in southern parts of India but not in the north in 2004-06.