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181 results on '"Narangajavana, Jarunya"'

36. Integrative omics approaches revealed a crosstalk among phytohormones during tuberous root development in cassava

Author

Utsumi, Yoshinori, primary, Tanaka, Maho, additional, Utsumi, Chikako, additional, Takahashi, Satoshi, additional, Matsui, Akihiro, additional, Fukushima, Atsushi, additional, Kobayashi, Makoto, additional, Sasaki, Ryosuke, additional, Oikawa, Akira, additional, Kusano, Miyako, additional, Saito, Kazuki, additional, Kojima, Mikiko, additional, Sakakibara, Hitoshi, additional, Sojikul, Punchapat, additional, Narangajavana, Jarunya, additional, and Seki, Motoaki, additional

Published
2020
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41. Cryopreservation of Dendrobium cruentum Rchb. f. Seeds by D Cryo-plate and V Cryo-plate Techniques.

Author

PRASONGSOM, Sasikarn, THAMMASIRI, Kanchit, NARANGAJAVANA, Jarunya, THITAMADEE, Siripong, CHUENBOONNGARM, Ngarmnij, and PANVISAVAS, Nathinee

Subjects
DENDROBIUM, CRYOPRESERVATION of organs, tissues, etc., SEEDS, SEED viability, WILDLIFE conservation, SODIUM alginate
Abstract

Dendrobium cruentum Rchb. f. is a native Thai orchid species that has faced extinction because of its attractive characteristics. Consequently, conservation of this species is urgently needed. In this study, cryopreservation technique was applied to D. cruentum Rchb. f. seeds for long-term conservation. A successful protocol for D. cruentum Rchb. f. seed cryopreservation was developed by using D cryo-plate and V cryo-plate techniques. Seed viability was tested by TCC solution and 93.8 % of dyed seeds were shown. For cryo-plate technique, seeds were encapsulated over the cryo-plate by using 2 % (w/v) sodium alginate and polymerized with 100 mM CaCl2. Encapsulated seeds were desiccated by using a laminar airflow and PVS2 solution treatment with the same exposure time (0, 30, 60, 90, and 120 min). After cryopreservation, encapsulated seeds were cultured on modified VW agar medium. From the results, the maximum germination and regrowth percentage were observed; D cryo-plate technique with 60 min of dehydration time gave the highest germination (68.9 %) and regrowth (57.8 %). Thus, the excess of dehydration may cause the reduction of germination and plant regeneration. In conclusion, D cryo-plate technique proved to be appropriate for D. cruentum Rchb. f. seed cryopreservation. [ABSTRACT FROM AUTHOR]

Published
2020

42. Development and mapping of functional expressed sequence tag-derived simple sequence repeat markers in a rubber tree RRIM600 3 PB217 population

Author

Nirapathpongporn, Kanlaya, Kongsawadworakul, Panida, Viboonjun, Unshira, Teerawattanasuk, Kanikar, Chrestin, Hervé, Seguin, Marc, Clément-Demange, André, and Narangajavana, Jarunya

Subjects
Hevea brasiliensis, food and beverages, F30 - Génétique et amélioration des plantes
Abstract

Sets of polymorphic expressed sequence tag–simple sequence repeat (EST-SSR) markers from the rubber tree (Hevea brasiliensis) have been published by many researchers, but none has been specifically developed to study latex and wood yield traits. In this study, a total 10,321 rubber tree EST sequences, generated from suppression subtractive hybridization-cDNA libraries of bark and latex of high- and low-yielding clones, were used as sources for SSR searching. A total of 432 EST-SSR loci were identified and it was possible to design primer pairs for a subset of 298 EST-SSRs. The highest proportion of EST-SSRs was represented by dinucleotide repeats (46.6 %), followed by trinucleotide repeats (44.3 %). Based on BLASTX analysis, 234 ESTs (80 %) showed similarity to genes in NCBI databases and could be divided into 120 putative proteins with known function and 114 unknown proteins. To enhance the resolution of an existing linkage map from previous work on a rubber tree RRIM600 × PB217 population, 69 EST-SSR markers from the above set were tested to be integrated into the reference genetic map. The enriched map of 18 linkage groups spanned 2054.2 cM in length, showed an average genetic distance of 4.3 cM between adjacent markers, and included 63 new EST-SSR markers. The enhanced map from this study provides a basis for comparative mapping using PCR-based markers and identification of expressed genes possibly affecting important traits of interest.

Published
2016

44. The Histone Deacetylase Inhibitor Suberoylanilide Hydroxamic Acid Alleviates Salinity Stress in Cassava

Author

Patanun, Onsaya, primary, Ueda, Minoru, additional, Itouga, Misao, additional, Kato, Yukari, additional, Utsumi, Yoshinori, additional, Matsui, Akihiro, additional, Tanaka, Maho, additional, Utsumi, Chikako, additional, Sakakibara, Hitoshi, additional, Yoshida, Minoru, additional, Narangajavana, Jarunya, additional, and Seki, Motoaki, additional

Published
2017
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48. Functional analysis of promoter sequences from Hevea brasiliensis hevein genes, in rice and rubber tree [S05-32]

Author

Pujade-Renaud, Valérie, Arokiaraj, P., Jones, Heddwyn, Sanier, Christine, Cambillau, Laurence, Tharreau, Didier, Narangajavana, Jarunya, and Chrestin, Hervé

Subjects
Résistance génétique, Séquence nucléotidique, fungi, food and beverages, Transformation génétique, Agrobacterium, Oryza, F30 - Génétique et amélioration des plantes, Hevea brasiliensis, Expression des gènes
Abstract

The 5' upstream region of two highly homologous hevein genes from rubber tree (PHev2.1 and PHev2.3) were isolated. Hevein is a small lectine-like protein pertaining to the group of type IV Pathogenesis-Related proteins. In rubber tree, it is located in latex cells exclusively and participates in the latex coagulation processes. It has antifungal properties in vitro. The 1.8 kb PHev2.1 promoter sequence fused to the GUS reporter gene was introduced in rice by Agrobacterium-mediated transformation. It appeared to be functional both in leaves, roots and anthers. It was activated in response to mechanical wounding in leaves, both directly and systemically, raising the promoter activity level close to that of the 35S promoter. Additionally, a 1.2 to 1.6 fold activation was observed after inoculation with the rice pathogen Magnaporthe grisea. These results are in agreement with the presence on the sequence of potential regulatory elements involving biotic and abiotic stress signal transduction hormones. The 0.9 kb PHev2.3 promoter sequence and derived deleted -fragments, fused to the GUS reporter gene, were introduced in rubber tree callus by Agrobacterium-mediated transformation. All fragments were functional, both in callus and embryoids. Tissue specificity as well as the response to biotic and abiotic stresses will be analyzed in the regenerated transgenic plants. The regulation properties observed confirm the expected role of hevein in defense. Moreover, the functionality of the hevein promoter both in dicots (rubber tree) and monocots (rice) opens the doors for a utilization in various homologous or heterologous systems, in genetic engineering programs aiming to increase the protection against biotic or abiotic stresses. (Texte intégral)

Published
2003

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